Insect pest control container and insect pest control method using the same

Abstract

To provide an insect pest control container which is low cost without an electric power source, is easy to be handled, and can be used at ease.SOLUTION: An insect pest control container 10 includes a container body 1 which can contain water and has insect pest induction color, and an insect pest control material 3 which is disposed to contact with the water within the container body 1 in use, and includes titanium oxide particles and silver particles.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a pest control container (kit) and a pest control method using the same. [Background technology]

[0002] It is estimated that approximately 720 million people in the world, from tropical to temperate regions, are still infected with mosquito-borne infectious diseases each year, of which 2 to 4 million die. Effective vaccines and antiviral drugs for many of these infectious diseases are still in development. Under these circumstances, the only way to suppress infectious diseases is to develop a method to control mosquitoes that transmit malaria. However, even now, more than 100 years after it was discovered that malaria is transmitted by mosquitoes, no method effective against mosquitoes has been found.

[0003] As a method for exterminating mosquitoes, for example, a method disclosed in Patent Document 1 is known in which adult mosquitoes are exposed to an insect growth regulator such as a juvenile hormone-like active compound, and the adult mosquitoes lay eggs at the water's edge, thereby exterminating the mosquito larvae. However, there are concerns about the impact of the juvenile hormone-like active compound used in this method on the ecosystem.

[0004] Also, Patent Document 2 is known as a method for preventing the breeding of mosquitoes. This method relates to a mosquito breeding prevention device that is equipped with a water storage section whose top is open to the atmosphere and has a drain outlet at the bottom, a drain valve that opens and closes the drain outlet, and a drive control means for the drain valve, and is characterized in that on the day that water is stored in the water storage section, the drain valve is driven after a certain time has elapsed to drain the water from the water storage section. However, this method requires a drive device and has a complicated configuration, and is not practical in areas where mosquitoes breed widely. In addition, spraying insecticides or applying insect repellents to the skin is also common, but these methods are only temporary, and the continuous use of organic compounds, which are the active ingredients in many cases, is not desirable from the perspective of health or the environment. Furthermore, there is a concern that chemical methods of killing insects may lead to the development of drug-resistant mosquitoes.

[0005] Patent Document 3, filed by the present applicant, is an example of a method for preventing the breeding of mosquitoes that has little impact on the environment and is easy to handle. This method relates to effectively killing mosquitoes and suppressing the breeding of mosquitoes by bringing composite particles containing titanium oxide particles, metal particles, and calcium phosphate particles into contact with or in close proximity to mosquitoes or mosquito eggs. This method has solved the problems of Patent Documents 1 and 2. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Application Publication No. 5-320001 [Patent Document 2] JP 2003-144031 A [Patent Document 3] International Publication No. 2019-225051 Summary of the Invention [Problem to be solved by the invention]

[0007] In the course of conducting more specific studies on the implementation of the method according to Patent Document 3, the present inventors noticed that there were further problems to be solved. Mosquito-borne infectious diseases are more prevalent in tropical and subtropical regions, and the incidence rate tends to be high among economically poor people. Therefore, there has been a demand for a mosquito control container (kit) that can be used even in places where there is no power source, does not require a power source such as a battery, is low-cost, and is easy to handle. In addition, since mosquitoes often hide in human living spaces, there has been a demand for a mosquito control container (kit) that has almost no insecticide odor, has little impact on health, and is harmless even if accidentally ingested by infants and young children, for example, in living spaces such as living rooms and bedrooms. Furthermore, since mosquitoes also suck the blood of animals, there has been a demand for a mosquito control container (kit) that is harmless even if accidentally ingested by animals and can be handled safely and with peace of mind in pet shops and livestock farms such as pig farms. In view of the above, an object of the present invention is to provide a pest control container (kit) that does not require a power source, is low-cost, easy to handle, and can be used safely. [Means for solving the problem]

[0008] <1> The pest control container comprises a container body having a pest-attracting color capable of holding water, and a pest control material containing titanium oxide particles and silver particles, which is placed inside the container body so as to come into contact with water during use. <2> The pest control container according to item 1, wherein the pest control material has a surface active function that reduces the surface energy of the pest and reduces the water repellency of the pest, thereby submerging the pest in water. <3> The pest control container according to claim 1 or 2, wherein the color of the container body is black or blue. <4> The pest control container according to item (1) or (2), further comprising an adhesive for capturing pests, the adhesive being disposed on at least a part of the inner wall of the container body. <5> A pest control container as described in <1> or <2>, wherein the pest is a vector for transmitting a zoonotic disease. <6> The pest control container according to <5>, wherein the pest is a mosquito. <7> Hydro silver titanium sheets (10.5g / m) cut into 6-8cm x 9-12cm pieces were used as pest control materials. 2 ) The pest control container according to <1> or <2>, comprising 2 to 6 sheets of the pest control container. <8> The pest control container according to item (1) or (2), further comprising a lattice-shaped lid having a plurality of through holes formed in an opening of the container body through which pests can enter. <9> 3. The pest control container according to claim 1, wherein the pest control material is in sheet form. <10> The pest control container according to <9>, wherein the sheet-like pest control material is colored black or blue. <11> The pest control method includes the steps of preparing a container body having a pest-attracting color, filling the inside of the container body with water, arranging a pest control material containing titanium oxide particles and metal particles inside the container body so that it comes into contact with the water, and submerging the pest by lowering the surface energy of the pest and lowering the water-repellent function of the pest. <12> The pest control method according to item 11, wherein the container body is black or blue in color to attract the pests to the container body. <13> The pest control method according to claim 11 or 12, further comprising a step of arranging an adhesive on a part of the inner wall of the container body so as to be capable of trapping pests. <14> The method for controlling insect pests according to claim 11 or 12, wherein the insect pests are insect pests that transmit zoonotic diseases. <15> The method for controlling pests according to claim 14, wherein the pests are mosquitoes. <16> The pest control method according to item 11 or 12, further comprising a step of placing the container body in an indoor or outdoor location without a power source. <17> The pest control method according to <11> or <12>, further comprising the steps of introducing an organic matter into the container body and reacting the pest control material with the organic matter to generate carbon dioxide. <18> As a pest control material, a hydrosilver titanium sheet (10.5g / m2) cut to 6-8cm x 9-12cm was added to 100cc of water. 2 The pest control method according to item <11> or <12>, further comprising adding 2 to 6 sheets of the pesticide. Effect of the Invention

[0009] According to the present invention, there is provided a pest control container (kit) that does not require a power source, is low-cost, easy to handle, and can be used safely. [Brief description of the drawings]

[0010] [Figure 1] FIG. 1 is a diagram showing a pest control container. [Diagram 2] FIG. 2 is a partially cutaway view of the container body of the pest control container. [Diagram 3] Fig. 3a shows the survival rates of larvae, pupae and adults when Aedes aegypti early stage larvae are placed in the container of the Test Example, Reference Example or Comparative Example, and Fig. 3b shows the survival rates of pupae and adults when Aedes aegypti late stage larvae are placed in the container of the Test Example, Reference Example or Comparative Example. [Figure 4] Fig. 4a shows the survival rates of larvae, pupae and adults when early stage larvae of Anopheles stephensi are placed in the container of the Test Example, Reference Example or Comparative Example, and Fig. 4b shows the survival rates of pupae and adults when late stage larvae of Anopheles stephensi are placed in the container of the Test Example, Reference Example or Comparative Example. [Diagram 5] Fig. 5a shows the submersion rate of female Aedes aegypti and Anopheles mosquitoes in the test example, reference example, and comparative example plots, and Fig. 5b shows the hatching rate of eggs laid by female Aedes aegypti and Anopheles mosquitoes in the test example, reference example, and comparative example plots. [Figure 6] FIG. 6 is a diagram showing the growth time of early and late stage larvae of Aedes aegypti and Anopheles stephensi until they become adults when placed in the containers of the Test Example, Reference Example, and Comparative Example. [Figure 7] 7a and 7b are diagrams showing the results of an experiment in which mosquitoes were submerged in a petri dish according to a test example. [Figure 8] FIG. 8 shows the results of contacting adult mosquitoes with the test liquid. [Figure 9] 9a and 9b are diagrams showing the results of an experiment in which mosquitoes were submerged in a petri dish according to a comparative example. [Figure 10] FIG. 10 shows the results of contacting adult mosquitoes with the comparative solution. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] The present invention will be described below with reference to embodiments, but the present invention is not limited to the following embodiments. In the drawings, parts having the same or similar functions are given the same or similar reference numerals and their description is omitted. However, the drawings are schematic. Therefore, specific dimensions, etc. should be determined in light of the following description. In addition, the drawings naturally include parts with different dimensional relationships and ratios.

[0012] [Pest control container] Fig. 1 is a diagram showing a pest control container (kit) 10. As shown in Fig. 1, the pest control container 10 comprises a container body 1 that is colored with a pest-attracting color and capable of holding water, and a pest control material 3 that is placed inside the container body so as to come into contact with the water during use. The pest control container 10 does not require a power source, is low-cost, easy to handle, and can be used safely.

[0013] The pest control material 3 preferably has a surface active function that reduces the surface energy of the pest and reduces the water repellency of the pest, thereby drowning the pest. This is because when pests approach waterside areas to drink water or lay eggs, and come into contact with the water containing the pest control material, the pests can be instantly submerged.

[0014] As the pest control material 3, various materials can be used without any particular limitation as long as they have the above-mentioned functions. For example, agents containing titanium oxide particles and silver particles, baking soda, etc. can be mentioned, but agents containing titanium oxide particles and silver particles are preferred. Specifically, a nonwoven fabric sheet (hereinafter also referred to as "hydrosilver titanium sheet") (manufactured by DR.C Pharmaceutical Co., Ltd.) containing a complex of hydroxyapatite powder, silver particles, and titanium oxide powder, an antifoaming agent, and phthalocyanine blue (pigment) can be used. In this case, a hydrosilver titanium sheet (10.5 g / m2) manufactured according to Manufacturing Examples 1 and 2 described below and cut into a size of about 6 to 8 cm x 9 to 12 cm, preferably 7 cm x 10 to 11 cm, is added to 100 cc of water. 2It is preferable to add 2 to 6 sheets of the mosquito net. If the amount is less than the lower limit, the mosquito net will not be submerged, and if the amount is more than the upper limit, the submersion effect will not change significantly.

[0015] The form of the pest control material 3 is not particularly limited as long as it exhibits the surface activity of the water contained in the container body 1, and for example, a sheet or bead-like material can be used, with the sheet-like material being preferred. It is more preferred that the pest control material is given a pest-attracting color, such as black or blue, because this increases the rate of pest extermination.

[0016] There are no particular limitations on the shape of the container body 1 as long as it can contain the pest control material 3 and water inside, but the dimensions in the X, Y, and Z directions of the water containing section of the container body 1 in Figure 1 can be X x Y x Z = 10-12 cm x 6-8 cm x 10-12 cm.

[0017] It is preferable that the container body 1 is colored in a way that attracts pests to the container body, because this increases the rate of pest extermination. There are no particular limitations on the color of the container body 1, so long as it is a color that attracts pests to the container body, but black or blue is preferable.

[0018] It is preferable that the open part of the container body 1 further includes a lattice-like lid 5 having a plurality of through holes 51 through which pests can enter, because this can prevent small children or pets from accidentally drinking the water placed in the container body.

[0019] Fig. 2 is a partially cutaway view of the container body 1. As shown in Fig. 2, at least a part of the inner wall of the container body 1 may further be provided with an adhesive material 11 for capturing pests. Even if mosquito larvae hatch and become adults inside the container, the mosquitoes have a tendency to fly out of the water by climbing up the wall, so by providing the adhesive material 11 on a part of the inner wall, the mosquitoes can be captured.

[0020] In the process of proceeding with the study towards the implementation of Patent Document 3, the inventor discovered that when a mosquito approaches for drinking water or laying eggs in water impregnated with hydro silver titanium sheet, it instantly submerges when it touches the water. After that, as a result of scientifically analyzing this phenomenon, it was elucidated that when a mosquito touches water impregnated with hydro silver titanium sheet, it is covered by the water and submerges. The present invention is based on the above discovery and analysis. The invention of Patent Document 3 focuses on the inhibition of hatching of mosquito eggs and the killing of mosquito larvae, but does not specifically suggest killing adult mosquitoes. It also does not suggest that mosquitoes should be submerged in water impregnated with a hydrosilver titanium sheet. This invention is truly surprising.

[0021] [Pests to be controlled] Pests to be controlled include those that transmit zoonotic diseases, such as mosquitoes. Specific examples of mosquitoes, the diseases they transmit, and their habitats are listed below: Asian tiger mosquito (dengue fever, chikungunya fever, Zika virus infection; temperate (Japan, etc.), subtropical, and tropical regions), Aedes aegypti (yellow fever, dengue fever, chikungunya fever, Zika virus infection; subtropical and tropical regions), Anopheles mosquitoes (malaria; temperate, subtropical and tropical regions), Culex mosquitoes (Japanese encephalitis, West Nile fever; temperate, subtropical and tropical regions).

[0022] [Pest control method] The pest control method will be described below, taking mosquitoes as an example of pests, in order to explain the action and effect of the pest control container. (a) First, a pest control container 10 having a pest-attracting color as shown in FIG. 1 is prepared. (B) Then, the pest control container 10 of FIG. 1 is placed in a place where mosquito control is required. The pest control container 10 does not require a power source, so it can be placed in any desired place, whether indoors or outdoors. The pest control container 10 does not use chemicals such as pesticides that are of concern for their effects on the human body, so it has almost no odor and has little effect on health. Therefore, it is harmless even if an infant or small child accidentally ingests it, so it can be used safely in the living room of the home. In addition, it is harmless even if an animal accidentally ingests it, so it can be used safely in pet shops and livestock farms such as pig farms. In the past, when pest control methods used pesticides, there was a concern about the emergence of resistant insects, but since the pest control container 10 does not use pesticides, it can be used safely without worrying about the emergence of resistant insects.

[0023] The pest control container 10 is preferably placed at night because mosquitoes have the habit of drinking water and being active at night. It is said that mosquitoes start sucking blood when the temperature is around 15°C, and become less active when the temperature exceeds 30°C. Considering the activity of mosquitoes, it is preferable to place the pest control container 10 in a space with a temperature of 25°C to 30°C, where mosquitoes are most active.

[0024] Depending on the control target, it may be preferable to select the placement position of the pest control container. For example, when the control target is Aedes albopictus, Aedes aegypti, or Culex mosquito, it is preferable to place the container body indoors. This is because Aedes albopictus and the like mainly breed indoors, so placing it indoors improves the capture rate. In that case, it is preferable to place it in a corner of the room. This is because mosquitoes have the tendency to hide in the shadows, so placing it in a corner of the room is expected to improve the capture rate. When the control target is Anopheles gambiae, it is preferable to place the container body outdoors. This is because Anopheles gambiae mainly breed outdoors, mainly in ponds and puddles, so placing it outdoors near the water's edge improves the capture rate.

[0025] (c) Next, water is poured into the container body. There are no particular limitations on the water, and tap water, well water, etc. can be used. The amount of water added may be enough to cover the pest control material 3, but considering that the water will evaporate, a larger amount is preferable. The specific amount of water added may be about 100 cc, assuming that the dimensions of the water storage section of the container body 1 in Fig. 1 in the X, Y, and Z directions are X x Y x Z = 11 cm x 7.5 cm x 11 cm. (iv) After that, a pest control material containing titanium oxide particles and metal particles is placed inside the container body so that it comes into contact with the water. When the pest control material dissolves in water, the surface activity effect of the water (super water repellency effect) is improved, and the mosquito submersion effect is improved. Hydrosilver titanium sheet (10.5 g / m2) cut to about 6-8 cm x 9-12 cm is placed in 100 cc of water. 2 ) is added.

[0026] (e) The mosquito is brought into contact with the water placed inside the container. When the mosquito comes into contact with the water, the surface energy of the mosquito decreases, and the water-repellent function of the mosquito decreases, so the mosquito can be submerged in water. As a result, the mosquito can be captured by submerging it in water. (F) After about two weeks, use water or a hydro silver titanium sheet (10.5g / m 2 ) is preferable. When the hydrosilver titanium sheet is added to water under the above-mentioned (ii) conditions, the pest control effect is about 2 weeks. Even if the larvae hatch in the water, their growth is suppressed more than if they were grown in normal water (see Test Example 3 in the Examples section below), so by changing the water etc. every 2 weeks, the larvae can be discarded before they become adults, and the emergence of adult mosquitoes can be suppressed. For convenience of explanation, each step is explained, but the order of steps (B), (C), and (D) is not particularly limited. For example, a pest control container containing water and a pest control agent in advance may be placed in a desired location, or water may be added after placing the pest control agent in the pest control container.

[0027] (Other embodiments) As described above, the present invention has been described by the embodiment, but the description and drawings forming a part of this disclosure should not be understood as limiting the present invention. Various alternative embodiments, examples and operating techniques will become apparent to those skilled in the art from this disclosure.

[0028] Although it has been described that water and a pest control agent are placed in the storage section inside the container body, the present invention is not limited thereto. For example, an organic matter that reacts with the pest control material to generate carbon dioxide may be further added to the water. This is because mosquitoes have a tendency to like the smell of carbon dioxide, and it is expected that the mosquito capture rate can be increased by attracting mosquitoes with the smell.

[0029] Although the embodiment in which the container body is provided with a pest-attracting color has been described, the present invention is not limited thereto. For example, the container body may be transparent or translucent, and a light-emitting body (e.g., an LED bulb or the like) may be disposed in a part of or near the container body to emit a pest-attracting color. Alternatively, the container body may be colored (pest-attracting color) and translucent, and a light-emitting body may be disposed in a part of or near the container body to emit a pest-attracting color. Thus, the present invention naturally includes various embodiments not described herein. Therefore, the technical scope of the present invention is defined only by the invention-specifying matters related to the scope of the claims appropriate from the above description. [Industrial Applicability]

[0030] The pest control container and the pest control method using the same do not require a power source, and therefore can be placed anywhere desired, whether indoors or outdoors. The pest control container and the pest control method using the same do not use chemicals such as pesticides that are of concern for their effects on the human body, so they have almost no odor and have little impact on health. Therefore, they are harmless even if infants accidentally ingest them, so they can be used safely in the living room of your home. They are also harmless even if animals accidentally ingest them, so they can be used safely in pet shops and livestock farms such as pig farms. In the past, in the case of pest control methods using pesticides, there was a concern about the emergence of resistant insects, but since the pest control container and the pest control method using the same do not use pesticides, they can be used safely without worrying about the emergence of resistant insects. EXAMPLES

[0031] The present invention will be described in more detail below based on test examples, although the scope of the present invention is not limited to these test examples.

[0032] [Production Example 1]: Production of composite particles In this production example, titanium oxide powder, silver powder and hydroxyapatite powder were used as raw material powders to produce composite particles M2 containing one or more titanium oxide particles, one or more silver particles and one or more hydroxyapatite particles.

[0033] The total amount of titanium oxide powder, silver powder, and hydroxyapatite powder was adjusted to 35 parts by mass relative to 65 parts by mass of water. The amount of polycarboxylic acid-based dispersant was adjusted to 0.5 parts by mass relative to 35 parts by mass of the total amount of titanium oxide powder, silver powder, and hydroxyapatite powder. The amount of titanium oxide powder was adjusted to about 30 parts by mass (29 to 31 parts by mass) relative to 1 part by mass of silver powder, and the amount of hydroxyapatite powder was adjusted to about 3 parts by mass (2.5 to 3.5 parts by mass) relative to 1 part by mass of silver powder.

[0034] Composite particles M2 were produced by drying the suspension (slurry) of the composite particles. The particle size of the composite particles M2 measured by the laser diffraction method was 200 to 500 nm. The median diameter (d50) of the composite particles M2 measured on a volume basis by the laser diffraction method was about 300 nm. The particle size was measured by the laser diffraction method using a commercially available particle size distribution measuring device, specifically, a laser diffraction / scattering type particle size distribution measuring device Partica LA-960V2 series (manufactured by HORIBA).

[0035] [Production Example 2]: Production of nonwoven fabric with composite particles A binder resin was added to the suspension (slurry) of composite particles M1 obtained in Production Example 1 to prepare a mixed liquid, and then a polyester spunbond nonwoven fabric was immersed in the mixed liquid to impregnate the nonwoven fabric with the mixed liquid. After immersion, the nonwoven fabric was removed from the mixed liquid and pressed with a roller to squeeze out excess mixed liquid. After pressing, the nonwoven fabric was dried at about 130°C for about 1 minute to produce composite particle-attached nonwoven fabric A1. A urethane-based resin (C3H7NO2 / NH2COOC2H5) was used as the binder resin.

[0036] By adjusting the concentrations of the composite particles M2 and the binder resin in the mixed solution, the total adhesion amount (total fixed amount) of the composite particles M2 and the binder resin per unit area of ​​the composite particle-attached nonwoven fabric A2 was increased to 10.5 g / m 2 was adjusted to. 10.5g / m 2 The breakdown is titanium dioxide 6.525g / m 2 , hydroxyapatite 0.750g / m 2 , silver 0.225g / m 2 , binder resin 3.00g / m 2 It was.

[0037] [Test Example 1] Mosquito larvae pupation and emergence inhibition test using hydrosilver titanium sheet In this test, a nonwoven fabric treated with hydro silver titanium and mosquito larvae were placed in a container filled with water, and compared with untreated nonwoven fabric to examine the effect of hydro silver titanium on the survival of mosquito larvae, pupae, and adults. In addition, the process of insect larvae becoming pupae is called "pupation", and the process of insects growing and becoming adults from pupae or larvae is called "emergence". 1. Test Method 1-1.Materials (1) Sample (i) Sample 1 As sample 1, a nonwoven fabric sheet (hereinafter referred to as "hydrosilver titanium sheet") (length 1m x width 1m) (manufactured by DR.C Pharmaceutical Co., Ltd.) containing 13.5 g of a composite of hydroxyapatite powder, silver particles, and titanium oxide powder, an antifoaming agent, and phthalocyanine blue (pigment), manufactured by the same method as in Manufacturing Examples 1 and 2, was used. Sample 1 corresponds to this embodiment. (ii) Sample 2 A blue nonwoven fabric sheet containing an antifoaming agent and phthalocyanine blue (pigment) was used as sample 2. Sample 2 corresponds to a reference example and was used to verify the effect of the sheet color. (iii) Sample 3 A white nonwoven fabric sheet containing no defoaming agent and no phthalocyanine blue (pigment) was used as sample 3. Sample 3 corresponds to a comparative example.

[0038] (2) Container (i) Test Example 100 mL of RO water was added to a 250 mL plastic container (diameter 7.6 cm, height 5.5 cm), and sample 1 cut to a size of 11 cm length x 11 cm width was placed in the container and immersed in the RO water. Sample 1 cut to a size of 11 cm length x 11 cm width contained 0.1635 g of hydroxyapatite powder, silver particles, and titanium oxide powder. The container containing sample 1 was designated the "test example." (ii) Reference Example A reference example was prepared in the same manner as sample 1, except that sample 2 cut to a size of 11 cm length x 11 cm width was placed in a 250 mL plastic container. The container containing sample 2 was designated the "reference example." (iii) Comparative example A comparative example was prepared in the same manner as sample 1, except that sample 3 cut to a size of 11 cm length x 11 cm width was placed in a 250 mL plastic container. The container containing sample 3 was designated the "comparative example." Hereinafter, in the figures, test examples are abbreviated as "HATs", reference examples are abbreviated as "BC", and comparative examples are abbreviated as "WC".

[0039] (3) Mosquitoes (i) Adult mosquitoes Laboratory-passaged Aedes aegypti (Bora Bora strain) and Anopheles dirus (Khao Mai Kaew strain) mosquitoes were used. Eggs of these mosquitoes were incubated in plastic trays (20 cm long × 30 cm wide × 5 cm high). After hatching, 200 larvae were reared in plastic trays containing 1500 mL of dechlorinated water and fed daily with fish food (Optimum Hi Pro Growth and Color, Perfect Companion Group, Bangkok, Thailand). Pupae were transferred to plastic containers and reared in cages (20 cm long × 20 cm wide × 30 cm high) until emergence. Adult mosquitoes were fed 5% sugar water on absorbent cotton. They were reared in an insect rearing room (in the Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University) at a temperature of 25±2°C and a relative humidity of 65±10%. Aedes aegypti mosquitoes live in subtropical and tropical regions, and transmit infectious diseases such as yellow fever, dengue fever, chikungunya fever, Zika virus infection, and filariasis. Anopheles mosquitoes live in temperate, subtropical, and tropical regions, and transmit infectious diseases such as malaria and filariasis. (ii) Early stage mosquito larvae 100 mL of RO water was added to a 250 mL plastic container, sample 1, sample 2, or sample 3 was placed in the container, and the container was submerged in water. Mosquito eggs were then placed in the container and soaked in water for 1 to 2 days. The hatched larvae (from the first to second instar larvae) were used as "prophase larvae." (iii) Late stage mosquito larvae Larvae hatched from the eggs in 100 mL of RO water were fed fish food (Optimum Hi Pro Growth and Color) daily and reared at a temperature of 25 ± 2°C until they reached the third to fourth instar larval stages. The third to fourth instar larvae were used as “late stage larvae”.

[0040] 1-2. Test procedure (1) Test conditions Twenty early or late stage mosquito larvae were placed in the containers of the test example, reference example, or comparative example. This treatment was carried out three times, with five containers for each condition. (2) Rearing conditions The larvae were fed 0.0046 ± 0.0008 g of fish food (Optimum Hi Pro Growth and Color) daily and reared at a temperature of 25 ± 2°C until they reached adulthood. (3) Counting the number of larvae, pupae, and adults and calculating survival rates The number of larvae, pupae, and adults in the containers was recorded daily, and the survival rate was calculated using the following formula: Survival rate = (number of larvae, pupae or adults) / (number of larvae tested) x 100

[0041] 2. Test Results Figure 3a shows the survival rates of larvae, pupae and adults when Aedes aegypti mosquito protozoa were placed in the container of the test example, the reference example or the comparative example. When Aedes aegypti protozoa were placed in the container of the test example, the survival rate of the protozoa, i.e., the rate at which they fully grew to adulthood, was 0.34%. In contrast, when early stage larvae of Aedes aegypti were placed in the reference container, the survival rate of the late stage larvae was 18%, the survival rate of the pupae was 16%, and the survival rate of the adults was 14%, with the survival rate gradually decreasing. When Aedes aegypti protolarvae were placed in the comparative container, the survival rate of the protolarvae was 93%, and these larvae grew to adulthood. That is, in the comparative container, the survival rate of Aedes aegypti adults was 93%.

[0042] FIG. 3b shows the survival rates of pupae and adults when late stage larvae of Aedes aegypti were placed in the containers of the test example, reference example, or comparative example. When late stage larvae of Aedes aegypti were placed in the test containers, the pupal survival rate was 9% and the adult survival rate was 9%. In contrast, in the case of the reference container, the survival rate of pupae was 52% and that of adults was 50%. In the comparative container, the survival rate of pupae was 96%, and the survival rate of adults was 96%.

[0043] FIG. 4a shows the results of the survival rates of larvae, pupae and adults when Anopheles mosquito early larvae were placed in the container of the test example, the reference example or the comparative example. When Anopheles mosquito early larvae were placed in the container of the test example, no pupae or adults survived, and the survival rates of pupae and adults were 0%. In contrast, when Anopheles mosquito early larvae were placed in the container of the reference example, the survival rate of pupae was 14% and the survival rate of adults was 14%. When Anopheles mosquito early larvae were placed in the container of the comparative example, the survival rate of late larvae was about 92%, the survival rate of pupae was about 87%, and the survival rate of adults was about 85%, which were significantly higher than those of the test example and the reference example.

[0044] Figure 4 4B shows the survival rates of pupae and adults when late stage larvae of Anopheles stephensi were placed in the container of the test example, the reference example, or the comparative example. When late stage larvae of Anopheles stephensi were placed in the container of the test example, no surviving pupae or adults were found, and the survival rates of pupae and adults were 0%. When late stage larvae of Anopheles stephensi were placed in the container of the reference example, a few surviving pupae were found, but no surviving adults were found. When late stage larvae of Anopheles stephensi were placed in the container of the comparative example, the survival rates of pupae and adults were approximately 90%, which was quite high. From the above, it was found that the container containing the hydro-silver-titanium sheet has an insecticidal effect against mosquito larvae regardless of the type of mosquito or the stage of the mosquito larvae, and can significantly suppress the occurrence of mosquitoes.

[0045] [Test Example 2] Hydro silver titanium sheet for mosquito submersion and egg hatching inhibition test In this test, a container containing nonwoven fabric treated with hydrosilver titanium and water was placed in a cage, and female mosquitoes that had been fertilized and sucked blood were placed in it.Then, by comparing with the case of untreated nonwoven fabric, the effect of nonwoven fabric treated with hydrosilver titanium on the submersion of mosquitoes, the number of eggs laid, and the hatching of laid eggs was examined. 1. Test Method 1-1.Materials (1) Sample (i) Sample 1 A hydrosilver titanium sheet (manufactured by DR.C Medical Co., Ltd.) was used as sample 1. Sample 1 corresponds to this embodiment. (ii) Sample 2 A blue nonwoven fabric sheet containing an antifoaming agent and phthalocyanine blue (pigment) was used as sample 2. Sample 2 corresponds to a reference example and was used to verify the effect of the sheet color. (iii) Sample 3 A white nonwoven fabric sheet containing no defoaming agent and no phthalocyanine blue (pigment) was used as sample 3. Sample 3 corresponds to a comparative example.

[0046] (2) Egg-laying container (i) Test Example For Aedes aegypti, 80 mL of RO water was added to a 250 mL plastic container, and one piece of sample 1 cut to a size of 11 cm in length x 11 cm in width and two pieces of sample 1 cut to a size of 5.5 cm in length x 5.5 cm in width were placed in the container and immersed in the RO water. For Anopheles gambiae, 80 mL of RO water was added to a 250 mL plastic container, and four pieces of Sample 1 cut to a size of 5.5 cm in length and 5.5 cm in width were placed in the container and immersed in the RO water. (ii) Reference Example The same procedure as in the Test Example was carried out as the Reference Example, except that Sample 2 was placed in a 250 mL plastic container. The container containing Sample 2 was designated the "Reference Example." (ii) Comparative Example A comparative example was prepared in the same manner as in the test example, except that sample 3 was placed in a 250 mL plastic container. The container containing sample 3 was designated the "comparative example."

[0047] (3) Mosquitoes (i) Adult mosquitoes Laboratory-passaged Aedes aegypti (Bora Bora strain) and Anopheles dirus (Khao Mai Kaew strain) mosquitoes were used. Eggs of these mosquitoes were incubated in plastic trays (20 cm long × 30 cm wide × 5 cm high). After hatching, 200 larvae were reared in plastic trays containing 1500 mL of dechlorinated water and fed daily with fish food (Optimum Hi Pro Growth and Color, Perfect Companion Group, Bangkok, Thailand). Pupae were transferred to plastic containers and reared in cages (20 cm long × 20 cm wide × 30 cm high) until they emerged. Adult mosquitoes were fed 5% sugar water on absorbent cotton. They were reared in an insectary (Insectary at the Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University) at 25 ± 2 °C and 65 ± 10% relative humidity.

[0048] 1-2. Test procedure (1) Test conditions (i) Submersion of female mosquitoes Thirty fully blood-fed inseminated female mosquitoes were allowed to suck blood for four days, and then the female mosquitoes were placed in a cage (length 30 cm x width 30 cm x height 40 cm). Next, a container of the test example, reference example, or comparative example was placed in the cage (the cage containing the container of the test example was designated the "test example area", the cage containing the container of the reference example was designated the "reference example area", and the cage containing the container of the comparative example was designated the "comparative example area"). After three days, the number of female mosquitoes submerged in the container and the number of eggs laid were recorded. This was repeated three times. (ii) Egg hatching The eggs of Anopheles mosquitoes laid on the nonwoven fabric in the containers of the Test Example, Reference Example, or Comparative Example were left in the same container until they hatched. The eggs of Aedes aegypti mosquitoes were dried at 27°C to 29°C for three days and then immersed in water in the container. The number of hatched larvae was counted and recorded for one week.

[0049] 2. Test Results Figure 5a shows the results of submersion of female mosquitoes of Aedes aegypti and Anopheles in the test, reference and comparative examples. For Aedes aegypti, the ratio of female mosquitoes submerged in the egg-laying container was 0.0667 in the test and 0.0556 in the reference, and no submerged mosquitoes were observed in the comparative example. For Anopheles, the ratio of female mosquitoes submerged in the egg-laying container was 0.5889 in the test, about 0.42 in the reference and 0.0111 in the comparative example.

[0050] The results of the number of eggs laid by female Aedes aegypti and Anopheles mosquitoes in the test example, reference example, and comparative example are shown in Table 1. The number of eggs laid by 90 female Aedes aegypti mosquitoes in the egg-laying container was 661 in the test example, 2905 in the reference example, and 6143 in the comparative example, with the test example being the lowest. The number of eggs laid by 90 female Anopheles mosquitoes in the egg-laying container was 1936 in the test example, 3311 in the reference example, and 8222 in the comparative example, with the test example being the lowest.

[0051] Figure 5b shows the hatching rate of eggs laid by female mosquitoes of Aedes aegypti and Anopheles in the test, reference and comparative examples. For Aedes aegypti, the hatching rate of eggs laid was 0.4311 in the test, 0.5232 in the reference and 0.7935 in the comparative examples. For Anopheles, the hatching rate of eggs laid was 0.2959 in the test, 0.68 in the reference and 0.7341 in the comparative examples. From the above, it has been found that a container containing the hydrosilver titanium sheet of the present invention can submerge mosquitoes and significantly suppress mosquito egg-laying and hatching rates, regardless of the type of mosquito.

[0052] [Table 1]

[0053] [Test Example 3] Mosquito larvae growth inhibition test using hydrosilver titanium sheet In this test, a nonwoven fabric treated with hydrosilver titanium and a mosquito pre-larva were placed in a container containing water, and the number of days it took for the surviving mosquito larvae to become adults was compared with that of untreated nonwoven fabric to examine the effect of the nonwoven fabric treated with hydrosilver titanium on the time it takes for the mosquito larvae to become adults. 1. Test Method 1-1.Materials (1) Sample The samples used were Sample 1, Sample 2, and Sample 3 described in Test Example 1. (2) Container As in Test Example 1, the containers used were those of the Test Example, Reference Example, and Comparative Example. (3) Mosquitoes As for the early and late stage larvae, as described in Test Example 1, early and late stage larvae of Aedes Aegypti (Bora Bora strain) and Anopheles dirus (Khao Mai Kaew strain) were used.

[0054] 1-2. Test procedure (1) Test conditions Twenty early or late stage mosquito larvae were placed in the containers of the test example, reference example, and comparative example. This treatment was carried out three times, with five containers for each condition. (2) Rearing conditions Larvae were fed 0.0046 ± 0.0008 g of fish food (Optimum Hi Pro Growth and Color) daily and reared at a temperature of 25 ± 2°C until they reached adulthood. (3) Counting the number of larvae, pupae, and adults and calculating survival rates The number of larvae, pupae and adults in the containers was recorded daily.

[0055] 2. Test Results FIG. 6 shows the results of the growth time from early and late stage larvae of Aedes aegypti and Anopheles stephensi to adulthood when they were placed in the containers of the Test Example, Reference Example, and Comparative Example. For the early stage larvae of Aedes aegypti, the number of early stage larvae that survived in the container was one in the container of the test example, nine in the container of the reference example, and many survived in the container of the comparative example (Figure 6a). Therefore, the survival rate of the early stage larvae of Aedes aegypti placed in the container of the test example was significantly lower than that of the reference example and the comparative example. For the late stage larvae of Aedes aegypti, the number of late stage larvae that survived in the container of the test example was three, and many survived in the containers of the reference example and the comparative example (Figure 6b). Therefore, the survival rate of the late stage larvae of Aedes aegypti placed in the container of the test example was significantly lower than that of the reference example and the comparative example. The growth time of the late stage larvae of Aedes aegypti mosquitoes that survived in the container until they became adults was about 5 to 7 days in the container of the test example, the median growth time was about 4.5 days in the container of the reference example, and the median growth time was 5 days in the container of the comparative example (Figure 6b). Therefore, the growth time of the late stage larvae of Aedes aegypti mosquitoes placed in the container of the test example was longer than those placed in the containers of the reference example and the comparative example.

[0056] When Anopheles mosquito protolarvae were placed in the container of the test example, no protolarvae survived in the container. In contrast, six larvae survived in the container of the reference example, and many larvae survived in the container of the comparative example (Figure 6c). The growth time from Anopheles mosquito protolarvae to adulthood could not be calculated because no larvae survived in the container of the test example, but in the container of the reference example, it was about 9 to 14 days, and in the container of the comparative example, the median growth time was about 10 days. When late stage larvae of Anopheles stephensi were placed in the container of the test example or reference example, no late stage larvae survived in the container. In contrast, many larvae survived in the container of the comparative example (Figure 6d). The growth time of late stage larvae of Anopheles stephensi until they became adults could not be calculated because no larvae survived in the containers of the test example and reference example. From the above, it has been found that the container containing the hydrosilver titanium sheet of the present invention has an insecticidal effect against mosquito larvae and has the effect of lengthening the growth time of mosquito larvae until they become adults, so that the occurrence of mosquitoes can be significantly suppressed.

[0057] [Test Example 4] Mosquito submersion test using Hydro Silver Titanium Sheet In this test, mosquitoes were exposed to water collected from a container containing water and nonwoven fabric treated with hydrosilver titanium, and the effect of the nonwoven fabric treated with hydrosilver titanium on the submersion of mosquitoes and their familiarity with water was examined by comparing the results with those of contact with untreated water. 1. Test Method 1-1.Materials (1) Sample A hydrosilver titanium sheet (manufactured by DR.C Pharmaceuticals Co., Ltd.) cut to a length of 4 cm and a width of 4 cm was used. (2) Container (i) Test Example 4 mL of ultrapure water (MilliQ water) was added to a 3 cm diameter dish, and the finely cut sample was placed in the dish and immersed in the MilliQ water. The water collected from the container in the test example was used as the "test liquid." (ii) Comparative Example 4 mL of MilliQ water was added to a 3 cm diameter petri dish. The water collected from the comparative example container was designated as the "comparison liquid."

[0058] 1-2. Test procedure (1) Test conditions (i) One adult mosquito was placed in the petri dish of the test example or comparative example, and it was observed whether it was submerged in water. (ii) The test liquid or comparative liquid collected from the test examples or comparative examples was dropped onto a glass surface with a dropper, and adult mosquitoes were brought into contact with the dropped test liquid or comparative liquid to observe whether the mosquitoes were repelled by the test liquid or comparative liquid.

[0059] 2. Test Results 7 and 9 show the results of mosquitoes being submerged in petri dishes in the test examples and comparative examples. As shown in Figure 7, the mosquitoes submerged in the water in the petri dish of the test example, whereas as shown in Figure 9, the mosquitoes did not submerge in the water in the petri dish of the comparative example. Figures 8 and 10 show the results of bringing adult mosquitoes into contact with the test solution or the comparison solution. As shown in Figure 8, when mosquitoes were exposed to the test liquid, they were not repelled by the test liquid and were able to adapt to it. On the other hand, as shown in Figure 10, when the mosquitoes were exposed to the comparison liquid, they were repelled by the comparison water and did not adapt to the water well.

[0060] [Test Example 5] Safety test of the water quality of the container containing the Hydro Silver Titanium sheet In this test, the water quality of the container containing nonwoven fabric treated with hydrosilver titanium was examined to verify its safety. 1. Test Method 1-1.Materials (1) Sample A hydro-silver-titanium sheet (manufactured by DR.C Pharmaceuticals Co., Ltd.) was used.

[0061] 1-2. Test procedure (1) Test conditions Pure water was added to the container, the sample was placed in the water and immersed, and the sample was left to stand for 12 hours, 14 hours and 72 hours before conducting a water quality test. (2) Test location Nagano Prefectural Industrial Technology Center

[0062] 2. Test Results The amount of detected elements and ion species after 72 hours was compared with the water quality standards for drinking water (when drinking water is supplied entirely or partially from water sources other than tap water or groundwater) and the results are shown in Table 2. The nitrate nitrogen and nitrite nitrogen of the pure water poured into the container containing sample 1 were 0.038 mg / l, copper and its compounds were 0.0004 mg / l, sodium and its compounds were 1.656 mg / l, and calcium, magnesium, etc. (hardness) were 1.607 mg / l, which were within the water quality standards for drinking water. Therefore, it was found that the pure water poured into the container containing the hydrosilver titanium sheet does not impair safety.

[0063] [Table 2] [Explanation of symbols]

[0064] 1 Container body 3. Pest control materials 5 Lattice lid 8 water 10 Pest control containers

Claims

1. A container body capable of storing water and having a pest-attracting color; A pest control container comprising: a pest control material containing titanium oxide particles and silver particles, the pest control material being placed inside the container body so as to come into contact with water during use.

2. 2. The pest control container according to claim 1, wherein the pest control material has a surface active function that reduces the surface energy of the pest and reduces the water repellency of the pest, thereby submerging the pest in water.

3. 3. The pest control container according to claim 1, wherein the container body is black or blue in color.

4. 3. The pest control container according to claim 1, further comprising an adhesive material for capturing pests, the adhesive material being disposed on at least a portion of the inner wall of the container body.

5. 3. The pest control container according to claim 1, wherein the pest is a vector for a zoonotic disease.

6. 6. The pest control container according to claim 5, wherein the pest is a mosquito.

7. As the pest control material, a hydrosilver titanium sheet (10.5 g / m) cut into 6-8 cm x 9-12 cm was used. ２ 3. The pest control container according to claim 1 or 2, comprising 2 to 6 sheets of the pest control container.

8. 3. The pest control container according to claim 1, further comprising a lattice-shaped lid having a plurality of through holes formed in the open portion of the container body through which pests can enter.

9. 3. The pest control container according to claim 1, wherein the pest control material is in the form of a sheet.

10. 10. The pest control container according to claim 9, wherein the sheet-like pest control material is colored black or blue.

11. Prepare a container body with a pest-attracting color, Fill the inside of the container body with water, A pest control method comprising the steps of: placing a pest control material containing titanium oxide particles and metal particles inside the container body so as to be in contact with the water; and submerging the pest by reducing the surface energy of the pest and reducing the water-repellent function of the pest.

12. The pest control method according to claim 11, wherein the container body is black or blue in color to attract the pests to the container body.

13. The pest control method according to claim 11 or 12, further comprising the step of arranging an adhesive material on a part of the inner wall of the container body so as to be capable of trapping the pest.

14. The method for controlling insects according to claim 11 or 12, wherein the insect pest is an insect pest that transmits a zoonotic disease.

15. The method for controlling insects according to claim 14, wherein the insect pest is a mosquito.

16. The pest control method according to claim 11 or 12, further comprising a step of placing the container body in an indoor or outdoor location where there is no power source.

17. 13. The pest control method according to claim 11 or 12, further comprising the step of introducing an organic matter into the inside of the container body and reacting the pest control material with the organic matter to generate carbon dioxide.

18. As the pest control material, a hydrosilver titanium sheet (10.5 g / m) cut into 6-8 cm x 9-12 cm pieces was mixed with 100 cc of water. ２ The pest control method according to claim 11 or 12, wherein 2 to 6 sheets of the pesticide are added.

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