Structure of the reagent disc plate for drug susceptibility testing

Abstract

To provide a structure of a reagent disk plate in a drug susceptibility test that simplifies the procedure for advance preparation of the test, distributes specimens uniformly and appropriately, prevents cross-infection of specimens, and prevents damage and contamination of the medium due to excessive gas. The device comprises a test medium 1, at least two support restriction sections 112, a central injection section 2, multiple microchannels 3, multiple reaction channels 4, multiple preset reagents 41 specifically for AST, multiple overflow channels 5, multiple overflow channels 6, multiple converging sections 51, and multiple exhaust holes 7. Before the test medium is sealed, freeze-dried preset reagents are placed in the reaction channels. When the user injects a sample into the central injection section, the test medium is placed in the testing device. Upon activation, the sample flows uniformly from the microchannels to the reaction channels under the action of centrifugal force. If the sample content in the reaction channel exceeds its capacity, the sample flows into the overflow channel due to the converging section of the overflow channel, and gas generated during the reaction is discharged through the exhaust hole.

Description

[Technical Field]

[0001] A reagent disc plate structure for drug susceptibility testing that simplifies the pre-test preparation procedures, allows multiple drug susceptibility tests to be performed simultaneously, distributes samples evenly and appropriately, has space and exhaust holes for samples to react independently, is easy to operate, has uniform concentration, sufficient bacterial quantity, prevents cross-infection of samples, and reduces indeterminacy due to artificial operating methods. [Background technology]

[0002] Antimicrobial susceptibility testing (AST), also known as antimicrobial susceptibility testing, aims to assess the susceptibility of bacteria, fungi, or other microorganisms to antimicrobial drugs (e.g., antibiotics) so that the most effective treatment can be selected.

[0003] In the traditional AST process, bacteria are first cultured and diluted to a usable concentration, and then a culture dish is prepared. The diluted bacterial solution is then applied dropwise to the culture dish, and a paper disk containing a predetermined concentration of antibiotic is placed on the culture dish and allowed to grow and react for a certain period of time at an appropriate temperature. Finally, the size of the inhibition zone is measured, and the effectiveness of the antibiotic against the bacterial species is recorded and determined visually.

[0004] Currently, a test device and its disc plate for the rapid AST test are commercially available, and the procedure is as follows: first, a sample is collected and diluted, then an indicator is dropped into the bacterial solution and mixed, and then the sample is poured into the disc plate. Finally, the sample is placed in the test device to react, and the reaction is judged by the color of the reagent when it is complete.

[0005] However, when using the above-mentioned AST test device and its disc plate, the following problems and drawbacks exist, and improvements are desired.

[0006] First, although this procedure is faster than the conventional process, it is prone to problems due to individual operating methods, and uneven concentration and staining of the indicator can make it difficult to interpret the results. In addition, the staff who actually operate the system must follow four to five steps, which increases the risk of contamination and infection.

[0007] Second, the volume of each reaction groove in the disc plate is less than 20 μl, so the amount of bacteria during the reaction is too small, preventing the pathogenic bacteria from growing normally, or the antibiotics have a strong effect on the bacteria, preventing them from growing normally, making the test results of the disc plate indeterminable.

[0008] Third, because the reaction grooves in the disc plate are interconnected, cross-infection is likely to occur. Furthermore, natural diffusion from the first reaction groove to the last reaction groove can lead to uneven concentrations of bacterial liquid in the reaction grooves, differences in the amount of bacteria, and mutual influence between the indicator and sample in each reaction groove, making it impossible to determine the results.

[0009] Fourth, the disc plate is not designed with an exhaust hole. Bacteria generate gas during the reaction process, and because the space inside the disc plate is sealed, if the gas is not exhausted in a timely manner, the more bacteria there are, the more gas there will be, which will push up the membrane on the disc plate, damaging the disc plate and causing leakage and even contamination.

[0010] Therefore, how to solve the above-mentioned problems and drawbacks of the conventional technology is a topic that the applicant of the present invention and related companies in this industry are eager to research and improve. Summary of the Invention [Problem to be solved by the invention]

[0011] Therefore, taking into account the above-mentioned shortcomings, the applicant of the present invention collected relevant materials, conducted evaluations and studies from various angles, and, through many years of experience accumulated in this industry and continuous prototyping and improvement, developed a reagent disc plate structure for drug susceptibility testing that simplifies the pre-test preparation procedures, allows multiple drug susceptibility tests to be performed simultaneously, distributes samples evenly and appropriately, and has space and exhaust holes for samples to react independently, is easy to operate, has uniform concentration, sufficient bacterial quantity, prevents cross-infection of samples, and reduces indeterminacy due to artificial operating methods.

[0012] The present invention aims to provide a reagent disc plate structure for drug susceptibility testing that utilizes the independence of the radially spaced arrangement of microchannels, allowing reaction tests to be performed in a testing device simply by injecting a sample from a single central injection port, thereby greatly simplifying the operating procedure and uniformly distributing the sample volume.

[0013] Another objective of the present invention is to provide a reagent disc plate structure for drug susceptibility testing that can eliminate the need for manual reagent addition by placing the same amount of preset reagent in each 20 μl to 80 μl reaction well, ensuring uniformity in the amount and concentration of reagent in each reaction well, and matching the amount of bacterial solution in each reaction well, thereby improving the completeness and accuracy of AST test results.

[0014] The present invention further aims to provide a reagent disc plate structure for drug susceptibility testing that utilizes the design of an overflow channel and overflow groove, allowing the sample volume to flow into the overflow groove when it is greater than the volume of the reaction groove, thereby effectively controlling the amount of bacteria, improving the accuracy of the experiment, and preventing cross-infection due to backflow of the bacterial liquid.

[0015] Another object of the present invention is to provide a reagent disc plate structure for drug susceptibility testing that utilizes a vent design to allow gas generated during the reaction process to be discharged, thereby preventing damage to the test medium due to excess gas and indirectly preventing infection due to liquid leakage. [Means for solving the problem]

[0016] To achieve the above-mentioned objectives, the structure of the present invention comprises a test medium, at least two support and limiting sections, a central injection section for injecting a sample, a plurality of radially arranged and independent microchannels, a plurality of reaction channels, a plurality of preset reagents specifically for AST, a plurality of overflow channels, a plurality of overflow channels, a plurality of tapered sections, and a plurality of exhaust holes. The central injection section is disposed in the test medium, and the plurality of support and limiting sections are formed within the test medium. The microchannels are arranged radially spaced apart on the sides of the central injection section, and each microchannel communicates with the central injection section. The reaction channels are respectively disposed on each microchannel, and the preset reagents are disposed in each reaction channel. The overflow channels are respectively formed on one side of each reaction channel, away from the microchannels, and communicate with each reaction channel. The overflow channels are respectively formed on one side of each overflow channel, and communicate with each overflow channel. The plurality of tapered portions are formed on the respective overflow channels, and the plurality of exhaust holes are provided on one side of the overflow grooves and communicate with the overflow grooves.

[0017] When a user performs a drug susceptibility test using this device, the test medium's reaction channel is filled with pre-filled lyophilized reagents. The user simply injects the sample through the central injection port, placing the test medium into the test device and ready to start. Furthermore, the support and limiting portion controls the insertion depth of the injection tool during injection, preventing damage to the sealing film while ensuring complete and reliable injection of the sample. This greatly simplifies the pre-injection procedure and essentially eliminates problems caused by manual operation. As the test medium rotates, centrifugal force causes the sample to flow through the radially spaced microchannels into each reaction channel, each with a volume ranging from 20 μl to 80 μl. After the sample has completely flowed out of the central injection port, if the sample content in each reaction channel exceeds the volume of the reaction channel, the overflow sample flows into the corresponding overflow channel through the corresponding overflow channel. The tapered portion design reduces the maximum inflow rate into the overflow channel. The sample then reacts with the preset reagents in each reaction well, and the gas generated during the reaction is exhausted from the test medium through the exhaust port. In this way, the sample can be evenly distributed to each microchannel, and the amount of sample in each reaction well can be stably controlled. Furthermore, the pre-arrangement of the freeze-dried preset reagents in the reaction wells makes it easy to control all conditions, improving the completeness and accuracy of the experiment. The independent channel and exhaust port design also effectively prevents cross-infection. [Effects of the Invention]

[0018] The above technology solves the problems of conventional AST testing devices and their disc plates, such as complicated preparatory procedures, manual operation methods, uneven bacterial concentration, insufficient bacterial quantity, inability to discharge excess gas, insufficient test accuracy, and a tendency for cross-infection, thereby achieving the practical inventiveness of the above advantages. [Brief explanation of the drawings]

[0019] [Figure 1] FIG. 1 is a perspective view of a first preferred embodiment of the present invention. [Figure 2] FIG. 2 is a diagram showing the state in which the reagents are pre-arranged in the preferred embodiment 1 of the present invention. [Figure 3] FIG. 2 is a diagram illustrating the injection of a sample in the first preferred embodiment of the present invention. [Figure 4] FIG. 2 is a diagram showing the split flow of the sample in the first preferred embodiment of the present invention. [Figure 5] FIG. 2 is a view showing the state in which the specimen has overflowed in the preferred embodiment 1 of the present invention. [Figure 6] FIG. 1 shows the reaction in preferred embodiment 1 of the present invention. [Figure 7] FIG. 2 is an exploded view of the second preferred embodiment of the present invention. [Figure 8] FIG. 10 is an exploded view of the third preferred embodiment of the present invention. [Figure 9] FIG. 10 is a perspective cross-sectional view of a fourth preferred embodiment of the present invention. [Figure 10] FIG. 10 is a perspective view of a fifth preferred embodiment of the present invention. [Figure 11] FIG. 10 is a perspective cross-sectional view of the fifth preferred embodiment of the present invention viewed from another angle. [Figure 12] FIG. 10 is a plan view of a sixth preferred embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0020] Please refer to Figures 1 to 6. Figures 1 to 6 are perspective views showing the reaction in a preferred embodiment 1 of the present invention. As can be seen from the figures, the present invention comprises a test medium 1, at least two support and limiting portions 112, a central injection portion 2, a plurality of microchannels 3, a plurality of reaction grooves 4, a plurality of preset reagents 41, a plurality of overflow channels 5, a plurality of overflow grooves 6, a plurality of tapered portions 51, and a plurality of exhaust holes 7.

[0021] At least two support restraints 112 are formed in the test medium 1 and are used to support the test medium 1 .

[0022] The central injection section 2 is provided between the support restriction sections 112 and is used to inject a sample, and the support restriction sections 112 limit the depth to which an injection tool can be inserted.

[0023] The multiple microchannels 3 are arranged radially at intervals on the sides of the central injection section 2, and each is connected to the central injection section 2, so that when the test medium 1 rotates, the sample flows uniformly into each of the microchannels 3 due to centrifugal force.

[0024] A plurality of reaction grooves 4 are provided in each of the microchannels 3, and the volume of each reaction groove 4 is 20 μl to 80 μl.

[0025] A plurality of preset reagents 41 are placed in each of the reaction wells 4, and each of the preset reagents 41 is a reagent dedicated to an antimicrobial susceptibility test (AST).

[0026] A plurality of overflow channels 5 are formed on one side of each reaction channel 4 away from each microchannel 3, and communicate with each reaction channel 4.

[0027] A plurality of overflow grooves 6 are formed on one side of each of the overflow channels 5 and communicate with each of the overflow channels 5 .

[0028] A plurality of tapered portions 51 are formed in each of the overflow channels 5 , and the openings on one side communicating with each of the reaction channels 4 are larger than the openings on one side communicating with each of the overflow channels 6 .

[0029] A plurality of exhaust holes 7 are provided on one side of the overflow groove 6, and communicate with the overflow groove 6 one after another.

[0030] The test medium 1 is a transparent circular disc plate, and the material is not limited. For example, it may be a transparent disc made of PC (polycarbonate) and comprise an upper disc plate 12 and a lower disc plate 11. The upper disc plate 12 and the lower disc plate 11 are supported by a plurality of support and limiting members 112, two of which are shown in this embodiment. The bottom center of the lower disc plate 11 is provided with a positioning member 111 that pivots to the testing device 8. The positioning member 111 is, for example, a recessed groove with an opening facing downward. The central injection portion 2 is formed in the central area between the upper disc plate 12 and the lower disc plate 11. The central injection portion 2 includes a temporary specimen storage area 21 between the upper disc plate 12 and the lower disc plate 11, and an injection port 22 formed in the center of the upper disc plate 12 and communicating with the temporary specimen storage area 21. Furthermore, the injection port 22 is formed between the support and limiting members 112, thereby controlling the depth at which an injection device is inserted. In this embodiment, the injection device is a dropper. For example, the plurality of microchannels 3 are 36 microchannels arranged at equal intervals. Therefore, the number of reaction channels 4, overflow channels 5, and overflow channels 6 is 36, and in this embodiment, they are all integrally molded and extend upward from the upper surface of the lower disc plate 11. For example, the exhaust holes 7 are 36 communicating overflow channels 6 and the passages on the surface of the upper disc plate 12. The preset reagent 41 is a combination of an acid-base indicator, an excipient, an antifreeze, and an antibiotic. For example, the indicator in this embodiment is an AST indicator such as resazurin or 2,3,5-triphenyltetrazolium chloride. The excipient is used to shape the preset reagent 41 and fix it in a concentrated manner within the reaction groove 4, thereby preventing the powdery structure of the preset reagent 41 from scattering to other places or adhering to the walls, and preventing uneven concentration in each reaction groove 4. The antifreeze agent is used in the freeze-drying process of the preset reagent 41, thereby keeping the preset reagent 41 in a low-temperature, dry state and not frozen. Antibiotics are the subject of AST experiments.The plurality of tapered portions 51 may be stepped or sloped, and in this embodiment, the stepped shape is used as an example. However, the corresponding shapes of the above-mentioned components are merely examples of preferred embodiments, and any shape having equivalent functions falls within the scope of the present invention and is not limited to the above examples.

[0031] The above description allows for a better understanding of the structure of this technology. This matching and combination structure further simplifies the pre-test preparation procedures, enables multiple drug susceptibility tests to be performed simultaneously, distributes samples evenly and appropriately, and provides space for independent sample reactions and an exhaust vent 7, resulting in advantages such as simple operation, uniform concentration, sufficient bacterial load, prevention of sample cross-infection, and reduction of indeterminacy due to manual operation. As can be seen from the diagram, the reaction well 4 contains pre-disposed preset reagents 41. Because the preset reagents 41 are pre-disposed before the test medium 1 is sealed, the user does not need to inspect the drug amount and concentration. Furthermore, because the preset reagents 41 are freeze-dried, the drug amount and concentration are more consistent. The preset reagents 41 also have high stability during antibiotic storage, effectively extending the shelf life and facilitating transportation. Thus, when performing an AST test, the user only needs to inject the sample through the central injection section 2, place the test medium 1 in the test device 8, and prepare for startup. This greatly simplifies the preparatory steps and essentially eliminates problems caused by manual operation. Furthermore, while the sample injection is typically performed using a dropper, the test medium 1 of the present invention includes a ring-shaped support limiter 112. The space surrounded by the support limiter 112 forms the injection port 22. Therefore, the user can simply connect the dropper to the injection port 22 and use the support limiter 112 to reduce the diameter of the central injection section 2. In this way, the dropper abuts against the support limiter 112, preventing it from touching the sealing film at the bottom. By controlling the insertion depth of the injection tool, it is possible to prevent the sealing film at the bottom from being dislodged or damaged by excessive insertion. Furthermore, by ensuring that the sample is injected reliably and completely into the test medium 1, side leakage can be reduced, improving the accuracy of the sample volume.

[0032] As the test medium 1 rotates, centrifugal force causes the sample to flow from multiple radially spaced microchannels 3 into each reaction groove 4. This design creates independent channels between each microchannel 3, ensuring that the sample flowing into any one microchannel 3 does not come into contact with the sample in another microchannel 3. Furthermore, because centrifugal force is used to drive the sample into the reaction groove 4 rather than natural diffusion, the sample in the central injection section 2 is uniformly dispersed into each microchannel 3, resulting in a uniform amount of drug.

[0033] Next, the sample flows from each microchannel 3 to the reaction well 4, which follows the same path. The volume of the reaction well 4 is 20 μl to 80 μl, which is sufficient compared to the conventional bacterial volume. If the bacterial volume is too low, the antibiotic will have a strong effect on the bacteria, competing with them and making the results indeterminable. However, if the bacterial volume is too high, the antibiotic will compete with the bacteria, making the test results worthless. Therefore, in this invention, by designing the reaction well 4 with a volume of 20 μl to 80 μl, an optimal bacterial volume can be provided, which is advantageous for conducting complete and accurate AST tests. Furthermore, in this embodiment, the optimal volume of the reaction well 4 is 50 μl. Naturally, if the sample content in each reaction well 4 exceeds the volume after all the sample has flowed out of the central injection port 2, the overflow portion will flow into the overflow well 6 via the overflow channel 5 and be simply isolated. Therefore, this invention can tolerate a small amount of bacterial solution overflowing from the reaction well 4, allowing for stable control of the sample volume in each reaction well 4. In addition, the freeze-dried preset reagents 41 allow all conditions to be easily controlled, enhancing the completeness and accuracy of the experiment.

[0034] Furthermore, in this embodiment, the extension direction of each microchannel 3 and each overflow channel 5 faces one side of the central injection section 2, and in the overflow channel 5 and microchannel 3 in the same reaction channel 4, the overflow channel 5 is located on one side of the microchannel 3 away from the rotation direction of the test medium 1. In other words, due to centrifugal force, the bacterial solution in the reaction channel 4 concentrates on one side of the reaction channel 4 away from the central injection section 2, but the opening of the overflow channel 5 communicating with the reaction channel 4 is located on one side of the reaction channel 4 closer to the central injection section 2. Therefore, the bacterial solution does not normally enter the overflow channel 5, and when the test medium 1 rotates clockwise, the overflow channel 5 is located on one side of the microchannel 3 in the counterclockwise direction. In this way, even if bacterial solution is present in the overflow channel 6, it is difficult for the bacterial solution to return to the reaction channel 4 via the overflow channel 5 during rotation, thereby preventing backflow. In particular, in this invention, the overflow channel 5 is provided with a tapered section 51, and the opening on one side of the tapered section 51 communicating with each of the reaction channels 4 is larger than the opening on one side of the overflow channel 6. That is, the size of this tapered inlet is used to reduce the maximum inflow volume into the overflow channel 6, or the width of the overflow channel 5 is designed to be narrower than the width of the microchannel 3, thereby slowing down the inflow speed into the overflow channel 5 and ensuring that the bacterial solution is thoroughly mixed with the preset reagent 41 before entering the overflow channel 6, thereby preventing a large amount of bacterial solution from flowing forward. This also ensures that the sample is thoroughly mixed with the preset reagent 41, and makes it difficult for the bacterial solution that has entered the overflow channel 6 to flow back into the microchannel 3, thereby effectively avoiding the problem of cross-infection.

[0035] The specimen then reacts with the preset reagents 41 in each reaction groove 4, and gas generated during the reaction process is exhausted from the test medium 1 through the exhaust holes 7, preventing excess gas from compressing and damaging the test medium 1 within the sealed disc plate. This indirectly prevents damage to the test medium 1 or the bacterial solution from spilling into other microchannels 3, thereby preventing contamination of the bacterial solution. Through the above operations, the AST test can be easily completed, and the remaining step is to determine the effectiveness of the antibiotic based on the color display of the indicator on the test medium 1.

[0036] Please also refer to FIG. 7, which is an exploded view of a second preferred embodiment of the present invention. As can be seen from the figure, this embodiment is essentially the same as the above-described embodiments, except that the test medium 1 includes at least one fixing portion 13 for fixing to the test device 8, and the test medium 1 includes an anti-misoperation portion 14 for confirming the installation direction of the test medium 1. The fixing portion 13 prevents the test medium 1 from coming off the test device 8 when rotated, and the anti-misoperation portion 14 confirms the installation direction of the test medium 1 and prevents the sample from spilling if it is installed in the opposite direction. In this embodiment, the fixing portion 13 is exemplified by a positioning rail on the side wall of the positioning portion 111. For example, it is an L-shaped rail, and the test device 8 is exemplified by a solid fixing part 82 connected to the fixing portion 13 at its axis 81. In this way, the test medium 1 can be effectively prevented from coming off. The misoperation prevention part 14 is, for example, an annular recessed groove at the bottom of the test medium 1. Therefore, the test device 8 is provided with a misoperation prevention indicator part 83 in the form of an annular convex rib that corresponds to the misoperation prevention part 14. If the user accidentally places the test medium 1 upside down, the misoperation prevention part 14 will be facing upward, and the misoperation prevention indicator part 83 will not be able to be inserted into the test medium 1, and the test medium 1 will not be able to be fixed in place. This warns the user that the test medium has been placed upside down.

[0037] Please also refer to Figure 8, which is an exploded view of a third preferred embodiment of the present invention. As can be seen from the figure, this embodiment is essentially the same as the above-described embodiments, except for the rotation method of the test medium 1 and the corresponding installation methods of the anti-misoperation device 14 and the fixing device 13. In this embodiment, the bottom of the test medium 1 does not have a positioning device used as an axis, and the test device 8 also does not have an axis pivotally attached to the test medium 1. Instead, the test medium 1 is positioned on the test device 8 by a circular fixing device 82 driven by a motorized roller from its outer edge to rotate. Therefore, the test medium 1 is placed inside the fixing device 82 and simultaneously driven to rotate by the fixing device 13, thereby preventing it from falling off during rotation. The anti-misoperation device 14 is, for example, a slit formed on the side edge of the lower disc plate 11. A convex anti-misoperation indicator 83 is formed protruding inward from the lower edge of the inner wall of the fixing device 82. As a result, since the upper disc plate 12 does not have an erroneous operation prevention part 14, if the user accidentally places the test medium 1 upside down, it will hit the operation prevention indicator part 83 and will not be able to be inserted, thereby achieving the purpose of preventing erroneous operation.

[0038] Please also refer to Figure 9, which is a perspective cross-sectional view of a fourth preferred embodiment of the present invention. As can be seen from the figure, this embodiment is similar to the above-mentioned embodiments, except that one side of the outer edge of the central injection section 2, which communicates with the plurality of microchannels 3, is provided with a flow-restricting groove 23. The flow-restricting groove 23 is a flat annular slit whose height is lower than the microchannels 3, and specifically, it is a drainage port recessed from the bottom edge of the wall of the central injection section 2 toward the inside of the wall. In this way, regardless of the amount of sample flowing through the micro-channel 3 at any given moment, the design of the flow-restricting groove 23 limits the amount of sample flowing into the micro-channel 3. Furthermore, since the flow-restricting groove 23 is connected to the inlets of each micro-channel 3 in a circular manner, even if a large amount of sample concentrates at the inlet of one of the micro-channels 3, the flow-restricting groove 23 not only immediately blocks it, but also guides the excess sample to both sides, allowing the sample in the central injection section 2 to collect in the flow-restricting groove 23 before slowly flowing into each micro-channel 3. This allows the sample in each reaction groove 4 to be distributed more evenly while also reducing the amount of sample remaining in the central injection section 2.

[0039] Please refer to Figures 10 and 11 at the same time. Figures 10 and 11 are a perspective view and a perspective cross-sectional view from a different angle of a preferred embodiment 5 of the present invention. As can be seen from the figures, this embodiment is almost the same as the above-mentioned embodiments, except that the cross-sectional area of ​​each microchannel 3 is a square with a size of 100 μm to 300 μm. Thus, the microchannel 3 is merely a shallow groove formed on the surface of the test medium 1. When connecting to a test device, the test medium 1 is placed upside down so that the microchannel 3 and the sealing film piece (upper disc plate 12) are positioned downward. The injection port 22 of the central injection unit 2 is located at the bottom of the test medium 1 and is exposed upward when placed upside down. Furthermore, by increasing the number of the support limiters 112 to four, the volume of the microchannel 3 is significantly reduced. In this embodiment, the cross-sectional area of ​​the microchannel 3 is 200 x 200 μm. 2For example, this can further improve the effects of slowing the flow of the bacterial solution into the overflow channel 5, mixing the bacterial solution with the preset reagent 41, preventing a large amount of bacterial solution from flowing forward, preventing the bacterial solution in the overflow channel 6 from flowing back into the microchannel 3, and preventing cross-infection. When operated in the reverse orientation, the reaction channel 4 and the overflow channel 6 are connected at the bottom, and due to the centrifugal force described above and the design of the overflow channel 5 being located on one side of the microchannel 3 away from the rotation direction of the test medium 1, the problem of the bacterial solution flowing into the overflow channel 6 basically does not occur.

[0040] Please also refer to Figure 12. Figure 12 is a plan view of a sixth preferred embodiment of the present invention. As can be seen from the figure, this embodiment is similar to the above-mentioned embodiments, except that the side of each reaction channel 4 that communicates with each overflow channel 5 is equipped with a collection groove 42. The collection groove 42 has a slightly protruding shape, forming a droplet shape when connected to the reaction channel 4. The tapered portion 51 is connected to one side of the collection groove 4. In this way, when the amount of bacterial solution in the reaction channel 4 exceeds the volume, the solution preferentially flows into the collection groove 42, quickly directing excess sample to flow into the overflow groove 6. This strikes a balance between limiting the unexpected inflow of sample into the overflow groove 6 and preventing excessive sample inflow into the overflow groove 6. The design of the collection groove 42 allows excess sample to quickly flow into the overflow groove 6 when it is present, and, combined with the tapered portion 51, prevents sample from accidentally flowing into the overflow groove 6 under normal conditions. This makes it possible to utilize the concentrating effect of the flow collecting grooves 42 to push out the gas bubbles from the rear of the overflow grooves 6 toward the exhaust holes 7, thereby improving the exhaust effect. [Explanation of symbols]

[0041] 1. Test medium 11 Lower disc plate 111 Positioning part 112 Support Restriction Section 12 Upper disc plate 13 Fixed part 14 Misoperation prevention section 2 Central injection part 21 Temporary specimen storage area 22 Inlet 23 Flow restriction groove 3 Microchannel 4 Reaction groove 41 Preset Reagents 42 Collecting groove 5 Overflow channel 51 Reduction section 6 Overflow groove 7 Exhaust vent 8 Test Equipment 81 Axial center 82 Fixing parts 83 Misoperation prevention indicator

Claims

1. A structure of a reagent disc plate in a drug susceptibility test, comprising a test medium, at least two support restriction parts, a central injection part, a plurality of microchannels, a plurality of reaction grooves, a plurality of preset reagents, a plurality of overflow channels, a plurality of overflow grooves, a plurality of tapered parts, and a plurality of exhaust holes, the at least two support restraints are formed within the test medium and are used to support the test medium; the central injection section is provided between the support restriction sections and is used for injecting a sample, and each of the support restriction sections limits the depth to which an injection device can be inserted; the plurality of microchannels are arranged radially at intervals on the sides of the central injection section, and each microchannel is in communication with the side of the central injection section, so that when the test medium rotates, the specimen flows uniformly into each microchannel by centrifugal force; the plurality of reaction grooves are provided in each of the microchannels, and the volume of each reaction groove is 20 μl to 80 μl; the plurality of preset reagents are disposed in the respective reaction wells, and each of the preset reagents is a reagent dedicated to an antimicrobial susceptibility test (AST); The overflow channels are formed on one side of each of the reaction channels, away from the microchannels, and communicate with each of the reaction channels. The plurality of overflow grooves are formed on one side of each of the overflow channels and communicate with each of the overflow channels, the plurality of tapered portions are formed in the overflow channels, and the openings on one side communicating with the reaction channels are larger than the openings on one side communicating with the overflow channels; The structure of the reagent disc plate for drug susceptibility testing, characterized in that the plurality of exhaust holes are respectively provided on one side of the overflow groove and communicate with the overflow groove.

2. The structure of a reagent disc plate in a drug susceptibility test described in claim 1, characterized in that the test medium has at least one fixing portion used to fix it to a test device, and the test medium has an anti-misoperation portion used to confirm the installation direction of the test medium.

3. 2. The structure of the reagent disc plate for drug susceptibility testing according to claim 1, wherein each of the reaction wells has a collecting groove on the side communicating with each of the overflow channels.

4. 2. The structure of the reagent disc plate in drug sensitivity testing according to claim 1, wherein the cross-sectional area of ​​each of the microchannels is a square of 100 μm to 300 μm.

5. 2. The structure of the reagent disc plate for drug sensitivity testing according to claim 1, wherein one side of the outer edge of the central injection part, which is connected to the plurality of microchannels, is provided with a flow restriction groove.

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