Method for Measuring Discharge Amount of Sub-Dispenser
The method of dissolving a dye in a volatile liquid, drying, and using optical measurement to create calibration curves accurately measures volatile liquid discharge amounts, addressing inaccuracies caused by volatilization in conventional methods.
JP7715214B2Active Publication Date: 2025-07-30SHIMADZU SEISAKUSHO LTD
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Patent Information
- Application Number
- JP2023573849
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2022-01-14
- Filing Date
- 2022-10-25
- Publication Date
- 2025-07-30
- Estimated Expiration
- 2042-10-25
AI Technical Summary
Technical Problem
Conventional methods for measuring the discharge amount of volatile liquids using dispensers are inaccurate due to volatilization effects, leading to significant errors in dispensed amounts, especially in the sub-microliter to microliter range.
Method used
A method involving dissolving a dye in a volatile liquid, drying the discharged liquid, adding a low-volatile liquid to dissolve the dye, and performing optical measurement to determine the discharge amount based on calibration curves.
Benefits of technology
Accurately measures the dispensed amount of volatile liquids by minimizing volatilization effects, allowing precise calibration without time-consuming channel-by-channel measurements.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention involves: dissolving a dye in a volatile liquid to prepare a dye-containing volatile liquid that contains the dye at a predetermined concentration (step 11); suctioning the dye-containing volatile liquid by a dispenser and discharging the same into a measurement vessel (step 12); drying the dye-containing volatile liquid discharged into the measurement vessel (step 13); adding, after the drying, a predetermined amount of a low-volatile liquid having a volatility lower than that of the volatile liquid to the measurement vessel to dissolve the dye and prepare an assessment liquid (step 14); conducting an optical measurement on the assessment liquid (step 15); and determining a discharged amount that is the volume of the dye-containing volatile liquid discharged into the measurement vessel, on the basis of a measured value of the assessment liquid obtained by the optical measurement, the added amount of the low-volatile liquid, and the concentration of the dye in the dye-containing volatile liquid (step 16). As a result, the amount of a volatile liquid dispensed by a dispenser such as a micropipette can be accurately measured.
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