Clonal tissue culture breeding method of Liquidambar formosana hance

Abstract

The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.

Description

technical field

[0001] The invention relates to a method for tissue culture and breeding, in particular to a method for tissue culture and breeding of sweetgum cedar clones, and belongs to the technical field of tree tissue culture and breeding. Background technique

[0002] Liquidambar ( Liquidambar formosana Hance) is a tree species of the genus Liquidambar in the family Hamamelis family. Large deciduous tree, up to 40m, with a maximum DBH of more than lm. Trunk straight; much branched, crown conical. Originating in my country, it is centered in the south of the Yangtze River Basin, reaching Taiwan in the east, Yunnan in the west, Hainan in the south, and Henan in the north. Wuzhishan is the most common in Hainan. Liquidambar is very suitable for building scenic forests in low mountains and hilly areas in southern my country, and towering trees are very magnificent in moist and fertile soil. It can also be planted as a shade tree in the garden. After the temperat...

Examples

Embodiment 1

[0043] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0044](1) Disinfection of explant materials: Take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1-2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 20 minutes, wash 6 times with sterile water, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 3 minutes, wash 6 times with sterile water, and then set aside;

[0045] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium, and after 6 days of cultivation, the axillary buds will start to germinate and new shoots will grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.05mg;

[0046] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, they were cut off, cultured on the subculture medium, and cultured on t...

Embodiment 2

[0051] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0052] (1) Disinfection of explant materials: Take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1-2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 30 minutes, wash 6 times with sterile water, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 5 minutes, wash 6 times with sterile water, and set aside;

[0053] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium, and after 15 days of cultivation, the axillary buds will start to germinate and new shoots will grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.5mg;

[0054] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, they were cut off, cultured on the subculture medium, and cultured on the p...

Embodiment 3

[0059] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0060] (1) Preparation of explant materials: take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1~2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 25 minutes, wash with sterile water 6 times, then use 0.1% mercuric chloride, 2-5 drops of Tween 80, treat for 4 minutes, wash with sterile water for 6 times and set aside;

[0061] (2) Induction of buds: inoculate the sterilized stem segments in step (1) on the bud induction medium, and after 10 days of cultivation, germination begins at the axillary buds and new shoots grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.25mg;

[0062] (3) Proliferation of buds: after culturing the new shoots grown in step (2) for 30 days, cut them off, then culture them on the subculture proliferation medium...

technical field

[0001] The invention relates to a method for tissue culture and breeding, in particular to a method for tissue culture and breeding of sweetgum cedar clones, and belongs to the technical field of tree tissue culture and breeding. Background technique

[0002] Liquidambar ( Liquidambar formosana Hance) is a tree species of the genus Liquidambar in the family Hamamelis family. Large deciduous tree, up to 40m, with a maximum DBH of more than lm. Trunk straight; much branched, crown conical. Originating in my country, it is centered in the south of the Yangtze River Basin, reaching Taiwan in the east, Yunnan in the west, Hainan in the south, and Henan in the north. Wuzhishan is the most common in Hainan. Liquidambar is very suitable for building scenic forests in low mountains and hilly areas in southern my country, and towering trees are very magnificent in moist and fertile soil. It can also be planted as a shade tree in the garden. After the temperat...

Examples

Embodiment 1

[0043] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0044](1) Disinfection of explant materials: Take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1-2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 20 minutes, wash 6 times with sterile water, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 3 minutes, wash 6 times with sterile water, and then set aside;

[0045] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium, and after 6 days of cultivation, the axillary buds will start to germinate and new shoots will grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.05mg;

[0046] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, they were cut off, cultured on the subculture medium, and cultured on t...

Embodiment 2

[0051] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0052] (1) Disinfection of explant materials: Take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1-2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 30 minutes, wash 6 times with sterile water, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 5 minutes, wash 6 times with sterile water, and set aside;

[0053] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium, and after 15 days of cultivation, the axillary buds will start to germinate and new shoots will grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.5mg;

[0054] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, they were cut off, cultured on the subculture medium, and cultured on the p...

Embodiment 3

[0059] Liquidambar clonal tissue culture breeding method is characterized in that: comprises the steps:

[0060] (1) Preparation of explant materials: take the young shoots of the year, remove the leaves, cut into sections, so that each section is a stem section with 1~2 axillary buds, and the stem sections are first sterilized with 0.2% and cleaned in an ultrasonic cleaner Treat for 25 minutes, wash with sterile water 6 times, then use 0.1% mercuric chloride, 2-5 drops of Tween 80, treat for 4 minutes, wash with sterile water for 6 times and set aside;

[0061] (2) Induction of buds: inoculate the sterilized stem segments in step (1) on the bud induction medium, and after 10 days of cultivation, germination begins at the axillary buds and new shoots grow. The induction medium is DCR 改 +6-BA 0.5mg+NAA 0.25mg;

[0062] (3) Proliferation of buds: after culturing the new shoots grown in step (2) for 30 days, cut them off, then culture them on the subculture proliferation medium...

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