Clonal tissue culture breeding method for camphor tree

Abstract

The invention relates to a clonal tissue culture breeding method for a camphor tree and belongs to the technical field of forest tree tissue culture. The clonal tissue culture breeding method comprises the steps of explant material sterilization, bud induction, bud propagation, rooting induction, rooted-seedling hardening, and transplantation and management of tissue-cultured seedling, and particularly comprises the following steps of: removing the leaves of an annual shoot; cutting the shoot into stems which are respectively provided with 1-2 axillary buds; sterilizing the stems; carrying out induced culture by an inducing medium; carrying out enrichment culture and rooting induction on the obtained new bud; hardening and naturalizing the obtained rooted seedling; and finally transplanting the naturalized seedling into a matrix which is sterilized, and managing the transplanted seedling. Seedling raising by the clonal tissue culture breeding method for the camphor tree, disclosed by the invention, is not affected by natural factors such as season and weather, and besides, production cost is low, land resources are saved, and seedling raising efficiency is improved; meanwhile, with adoption of the clonal tissue culture breeding method disclosed by the invention, the phenomenon of browning can be effectively prevented, the effective reproduction rate is high, the rooted seedling is grown regularly, the culture period is short, the survival rate for the transplanting of the tissue-cultured seedling is high, and the seedling stage culture period is short; and the clonal tissue culture breeding method is significant.

Description

technical field

[0001] The invention relates to a method for tissue culture and breeding, in particular to a method for tissue culture and breeding of camphor tree clones, and belongs to the technical field of forest tree tissue culture and breeding. Background technique

[0002] Camphor tree (Cinnamomum camphora (L.) Presl), belonging to the genus Cinnamomum camphora, is an evergreen tree and is a national second-class protected tree species. In South China, camphor tree is a well-known multi-purpose local precious tree species and economic tree species. It has a wide distribution range, a high degree of development and utilization, and is well-known to everyone. It has always been highly valued by people. In addition to being a precious material, it can also be used to extract borneol, camphor oil, etc. Its deep-processed products are widely used in medicine, optics and industry, and are closely related to people's production and life. The resources available for wood and...

Examples

Embodiment 1

[0052] Camphor tree clone tissue culture breeding method, comprises the steps:

[0053] (1) Disinfection of explant materials: remove the leaves from the young shoots of the current year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 3 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0054] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture, after 6-15 days of cultivation, germination begins at the axillary buds, and new shoots grow. The induction medium is DCR 改 +6-BA 0.3mg+NAA 0.05mg;

[0055] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, the new shoots are cut off, cultured on the subculture medium, and cultured on the proliferation medium for 6 days, clustered shoots will be formed. Proliferation ...

Embodiment 2

[0060] Camphor tree clone tissue culture breeding method, comprises the steps:

[0061] (1) Disinfection of explant materials: remove the leaves from the young shoots of the current year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 5 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0062] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture, after 6-15 days of cultivation, germination begins at the axillary buds, and new shoots grow. The induction medium is DCR 改 +6-BA0.3mg+NAA 0.5mg;

[0063] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, cut off the new shoots, and then culture them on the subculture proliferation medium. After culturing on the proliferation medium for 15 days, clustered buds will for...

Embodiment 3

[0068] Camphor tree clone tissue culture breeding method, comprises the steps:

[0069] (1) Disinfection of explant materials: remove the leaves from the young shoots of the year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 4 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0070] (2) Bud induction: Inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture. After 10 days of cultivation, germination begins at the axillary bud and new buds grow. The induction DCR 改 +6-BA 0.3mg+NAA 0.3mg;

[0071] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, the new shoots are cut off, cultured on the subculture medium, and cultured on the proliferation medium for 10 days, clustered shoots will be formed. Proliferation medium for DCR 改 +6-BA ...

technical field

[0001] The invention relates to a method for tissue culture and breeding, in particular to a method for tissue culture and breeding of camphor tree clones, and belongs to the technical field of forest tree tissue culture and breeding. Background technique

[0002] Camphor tree (Cinnamomum camphora (L.) Presl), belonging to the genus Cinnamomum camphora, is an evergreen tree and is a national second-class protected tree species. In South China, camphor tree is a well-known multi-purpose local precious tree species and economic tree species. It has a wide distribution range, a high degree of development and utilization, and is well-known to everyone. It has always been highly valued by people. In addition to being a precious material, it can also be used to extract borneol, camphor oil, etc. Its deep-processed products are widely used in medicine, optics and industry, and are closely related to people's production and life. The resources available for wood and...

Examples

Embodiment 1

[0052] Camphor tree clone tissue culture breeding method, comprises the steps:

[0053] (1) Disinfection of explant materials: remove the leaves from the young shoots of the current year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 3 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0054] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture, after 6-15 days of cultivation, germination begins at the axillary buds, and new shoots grow. The induction medium is DCR 改 +6-BA 0.3mg+NAA 0.05mg;

[0055] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, the new shoots are cut off, cultured on the subculture medium, and cultured on the proliferation medium for 6 days, clustered shoots will be formed. Proliferation ...

Embodiment 2

[0060] Camphor tree clone tissue culture breeding method, comprises the steps:

[0061] (1) Disinfection of explant materials: remove the leaves from the young shoots of the current year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 5 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0062] (2) Bud induction: inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture, after 6-15 days of cultivation, germination begins at the axillary buds, and new shoots grow. The induction medium is DCR 改 +6-BA0.3mg+NAA 0.5mg;

[0063] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, cut off the new shoots, and then culture them on the subculture proliferation medium. After culturing on the proliferation medium for 15 days, clustered buds will for...

Embodiment 3

[0068] Camphor tree clone tissue culture breeding method, comprises the steps:

[0069] (1) Disinfection of explant materials: remove the leaves from the young shoots of the year, and cut them into sections so that each section becomes a stem section with 1 to 2 axillary buds. Wash with water for 6 times, then use 0.1% mercury liter, 2-5 drops of Tween 80, treat for 4 minutes, wash with sterile water for 6 times, sterilize and set aside;

[0070] (2) Bud induction: Inoculate the sterilized stem section in step (1) on the bud induction medium for induction culture. After 10 days of cultivation, germination begins at the axillary bud and new buds grow. The induction DCR 改 +6-BA 0.3mg+NAA 0.3mg;

[0071] (3) Proliferation of buds: After culturing the new shoots grown in step (2) for 30 days, the new shoots are cut off, cultured on the subculture medium, and cultured on the proliferation medium for 10 days, clustered shoots will be formed. Proliferation medium for DCR 改 +6-BA ...

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