Specific marker linked with stripe rust resistance gene of Yilong Tuo wheat, and application of specific marker

Abstract

The invention relates to the agricultural biotechnology engineering and the field of disease-resistant genetic breeding of wheat, and in particular relates to a specific marker linked with a stripe rust resistance gene of Yilong Tuo wheat, a preparation method and application of the specific marker. The preparation method substantially comprises the following steps: adopting Taichang 29 / F2 group of the Yilong Tuo wheat as the mapping population according to the linkage separation rules; and acquiring an SSR (Simple Sequence Repeat) marker Xgpw 7342-350bp linked with the stripe rust resistance gene of the wheat according to the identification result of the stripe rust resistance in field and the linkage analysis of SSR marker data (forward primer: 5'-GCGGTGGCAACAACCTAC-3'), and reverse primer: 5'-GTGCATAAAGAGGGCAGAGC-3'). The marker can be used for assisting the selective breeding of the stripe rust resistance of the wheat, the shortcomings that in the conventional breeding, the stripe rust resistance identification of the wheat is easily influenced by the environment, the stability and the repeatability are poor, the workload is large, the time and manpower are wasted, and the breeding period is long, can be overcome, so that the selection method is simplified, the breeding efficiency is improved, and the breeding progress of the wheat stripe rust resistance variety is improved.

Description

technical field

[0001] The invention relates to the fields of agricultural biotechnology engineering and wheat disease-resistant genetic breeding, in particular to specific molecular markers linked with stripe rust-resistant genes of Yilongtuo wheat and the application thereof. Background technique

[0002] Wheat stripe rust caused by Puccinia striiformis Westend.f.sp.tritici (Pst) is one of the most severe typical airborne diseases of wheat. It exists, and occurs to varying degrees in more than 60 countries on all continents except Antarctica. China is the world's largest and relatively independent wheat stripe rust endemic area. Wheat stripe rust has always been one of the most important wheat diseases threatening the winter wheat regions and northwest spring wheat regions in Northwest, Southwest, Huanghuaihai and other places in my country. In recent years, the emergence and prevalence of the new toxic races Tiaozhong 32 and Tiaozhong 33 have exacerbated the trend of st...

Examples

Embodiment 1

[0050] 1. Test materials

[0051] Plant materials: The susceptible variety Taichang 29 was used as the female parent, and the resistant Sichuan local wheat Yilongtuomai was used as the male parent to construct F 2 Genetic Mapping Populations. In the main season of 2010, F 2 152 individual plants of the population were planted in the disease identification nursery of Wenjiang Experimental Base, Sichuan Agricultural University, Chengdu Campus, for resistance identification. Some leaves of each individual plant were taken at the seedling stage for SSR analysis. When ripe, harvest F 2:3 The seeds of the family were dried and preserved, and used for resistance identification in 2011.

[0052] Mixed Physiological Races of Stripe Rust: For F 2 group and F 2:3 The mixed physiological races of stripe rust identified by family resistance consisted of equal mixtures of three races 31, 32 and 33 in the strip.

[0053] 2. Identification of resistance

[0054] Field experiment desig...

Embodiment 2

[0080] 1. Test materials

[0081] Plant materials: the susceptible variety Taichang 29, the resistant variety Yilongtuomai, a local wheat variety from Sichuan, and the advanced generation (F 5 ) 4 wheat lines (L1-L4), 4 Sichuan local wheat varieties different from the disease-resistant variety Yilongtuomai, including: Bazhongtuomai, Kaijiang Baimai, Yibin Zhuermai, Hejiang reprinted wheat , 10 wheat breeding varieties that have no genetic relationship with the disease-resistant variety Yilongtuomai, including: Neimai 8, Neimai 9, Chuanmai 47, Kechengmai 2, Chuanmai 42, Chuannong 16, Chuannong Fu 5, Miannong 3, Mianyang 26, and Chuanmai 22. In the first season of 2011, the above materials were planted in the disease identification nursery of Wenjiang Experimental Base of Sichuan Agricultural University Chengdu Campus for resistance identification. Some leaves of each individual plant were taken at the seedling stage for SSR analysis.

[0082] Mixed physiological race of strip...

technical field

[0001] The invention relates to the fields of agricultural biotechnology engineering and wheat disease-resistant genetic breeding, in particular to specific molecular markers linked with stripe rust-resistant genes of Yilongtuo wheat and the application thereof. Background technique

[0002] Wheat stripe rust caused by Puccinia striiformis Westend.f.sp.tritici (Pst) is one of the most severe typical airborne diseases of wheat. It exists, and occurs to varying degrees in more than 60 countries on all continents except Antarctica. China is the world's largest and relatively independent wheat stripe rust endemic area. Wheat stripe rust has always been one of the most important wheat diseases threatening the winter wheat regions and northwest spring wheat regions in Northwest, Southwest, Huanghuaihai and other places in my country. In recent years, the emergence and prevalence of the new toxic races Tiaozhong 32 and Tiaozhong 33 have exacerbated the trend of st...

Examples

Embodiment 1

[0050] 1. Test materials

[0051] Plant materials: The susceptible variety Taichang 29 was used as the female parent, and the resistant Sichuan local wheat Yilongtuomai was used as the male parent to construct F 2 Genetic Mapping Populations. In the main season of 2010, F 2 152 individual plants of the population were planted in the disease identification nursery of Wenjiang Experimental Base, Sichuan Agricultural University, Chengdu Campus, for resistance identification. Some leaves of each individual plant were taken at the seedling stage for SSR analysis. When ripe, harvest F 2:3 The seeds of the family were dried and preserved, and used for resistance identification in 2011.

[0052] Mixed Physiological Races of Stripe Rust: For F 2 group and F 2:3 The mixed physiological races of stripe rust identified by family resistance consisted of equal mixtures of three races 31, 32 and 33 in the strip.

[0053] 2. Identification of resistance

[0054] Field experiment desig...

Embodiment 2

[0080] 1. Test materials

[0081] Plant materials: the susceptible variety Taichang 29, the resistant variety Yilongtuomai, a local wheat variety from Sichuan, and the advanced generation (F 5 ) 4 wheat lines (L1-L4), 4 Sichuan local wheat varieties different from the disease-resistant variety Yilongtuomai, including: Bazhongtuomai, Kaijiang Baimai, Yibin Zhuermai, Hejiang reprinted wheat , 10 wheat breeding varieties that have no genetic relationship with the disease-resistant variety Yilongtuomai, including: Neimai 8, Neimai 9, Chuanmai 47, Kechengmai 2, Chuanmai 42, Chuannong 16, Chuannong Fu 5, Miannong 3, Mianyang 26, and Chuanmai 22. In the first season of 2011, the above materials were planted in the disease identification nursery of Wenjiang Experimental Base of Sichuan Agricultural University Chengdu Campus for resistance identification. Some leaves of each individual plant were taken at the seedling stage for SSR analysis.

[0082] Mixed physiological race of strip...

Images