A method for increasing the content of γ-aminobutyric acid in germinated peanuts

Abstract

A method for increasing the content of γ-aminobutyric acid in germinated peanuts. Select well-grown peanuts, wash away the dust with clear water, then wash with distilled water, and drain; soak and disinfect with sodium hypochlorite solution, wash with distilled water, and place in a safe place. Under light conditions, soak in water; after the soaked peanuts are pulsed with strong light, wash them with distilled water, add water of the same quality as the peanuts, soak them in dark conditions to make them germinate; spray distilled water, and wait for the peanuts to germinate The sprouts grow to 10 mm to 25 mm to obtain germinated peanuts, and the enzymes are inactivated; they are vacuum freeze-dried and vacuum-packed, and stored in a cold storage below 10°C. Using pulsed strong light physical treatment, easy to operate, can increase the activity of enzymes, activate protease and glutamic acid decarboxylase in peanuts, accelerate protein decomposition, enhance the growth and stress resistance of peanut seeds, and the GABA content of germinated peanuts can reach More than 280mg / 100g.

Description

technical field

[0001] The invention relates to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts, in particular to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts by using pulsed strong light. Background technique

[0002] When plant seeds are exposed to adversities such as drought and flood, low temperature, hypoxia, heat shock, cold shock, and machinery, they often suffer from different degrees of damage, which leads to changes in plant physiological and biochemical processes, and then affects the production of nutrients. Under adversity conditions, the plant body will be harmed, such as the reduction of water absorption capacity of the plant body, the lack of water in the body, and the increase of growth inhibitory substances.

[0003] The metabolic intensity in plant seeds is closely related to their stress resistance. Appropriate intensity and physical energy can accelerate the metabolic process an...

Examples

Embodiment 1

[0020] Example 1

[0021] 1.1. Pre-treatment of peanuts

[0022] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 0.8% sodium hypochlorite solution for 10 minutes for disinfection Afterwards, wash with distilled water for 3 times, put it under the condition of 28°C and avoid light, soak in water for 8h;

[0023] 1.2. Peanut pulsed strong light treatment

[0024] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument will start stably. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 300J, the pulse distance is 11cm, the frequency is 1 time / s, and the time is 30s. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatme...

Embodiment 2

[0035] 1.1. Pre-treatment of peanuts

[0036] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 1.2% sodium hypochlorite solution for 15 minutes for disinfection Finally, wash it with distilled water for 5 times, place it in the dark at 32°C, and soak it in water for 12 hours;

[0037] 1.2. Peanut pulsed strong light treatment

[0038] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting steadily. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 100J, the pulse distance is 8cm, the frequency is 4 times / s, and the time is 3min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0039] 1.3 Germ...

Embodiment 3

[0044] 1.1. Pre-treatment of peanuts

[0045] Remove impurities, moldy grains and poorly mature peanuts from peanuts, select well-grown peanuts, wash off the dust with tap water, then wash with distilled water, and drain; soak in 1% sodium hypochlorite solution for 12 minutes for disinfection Finally, wash it with distilled water for 4 times, place it in the dark at 30°C, and soak it in water for 10 minutes;

[0046] 1.2. Peanut pulsed strong light treatment

[0047]Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting stably. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 500J, the pulse distance is 13cm, the frequency is 2 times / s, and the time is 1min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0048] 1.3 Germin...

technical field

[0001] The invention relates to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts, in particular to a method for increasing the content of gamma-aminobutyric acid in germinated peanuts by using pulsed strong light. Background technique

[0002] When plant seeds are exposed to adversities such as drought and flood, low temperature, hypoxia, heat shock, cold shock, and machinery, they often suffer from different degrees of damage, which leads to changes in plant physiological and biochemical processes, and then affects the production of nutrients. Under adversity conditions, the plant body will be harmed, such as the reduction of water absorption capacity of the plant body, the lack of water in the body, and the increase of growth inhibitory substances.

[0003] The metabolic intensity in plant seeds is closely related to their stress resistance. Appropriate intensity and physical energy can accelerate the metabolic process an...

Examples

Embodiment 1

[0020] Example 1

[0021] 1.1. Pre-treatment of peanuts

[0022] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 0.8% sodium hypochlorite solution for 10 minutes for disinfection Afterwards, wash with distilled water for 3 times, put it under the condition of 28°C and avoid light, soak in water for 8h;

[0023] 1.2. Peanut pulsed strong light treatment

[0024] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument will start stably. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 300J, the pulse distance is 11cm, the frequency is 1 time / s, and the time is 30s. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatme...

Embodiment 2

[0035] 1.1. Pre-treatment of peanuts

[0036] Remove impurities, moldy grains and peanuts with poor maturity in peanuts, select well-grown peanuts, wash away the dust with tap water, then wash with distilled water, drain; soak in 1.2% sodium hypochlorite solution for 15 minutes for disinfection Finally, wash it with distilled water for 5 times, place it in the dark at 32°C, and soak it in water for 12 hours;

[0037] 1.2. Peanut pulsed strong light treatment

[0038] Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting steadily. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 100J, the pulse distance is 8cm, the frequency is 4 times / s, and the time is 3min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0039] 1.3 Germ...

Embodiment 3

[0044] 1.1. Pre-treatment of peanuts

[0045] Remove impurities, moldy grains and poorly mature peanuts from peanuts, select well-grown peanuts, wash off the dust with tap water, then wash with distilled water, and drain; soak in 1% sodium hypochlorite solution for 12 minutes for disinfection Finally, wash it with distilled water for 4 times, place it in the dark at 30°C, and soak it in water for 10 minutes;

[0046] 1.2. Peanut pulsed strong light treatment

[0047]Open the pulsed strong light test cabinet, and after running for 10 minutes, the instrument keeps starting stably. Set the pulse parameters according to the experimental conditions. The pulse flash energy is 500J, the pulse distance is 13cm, the frequency is 2 times / s, and the time is 1min. Evenly distribute in the center of the sterile quartz plate directly under the lamp tube in the pulsed strong light experimental cabinet, close the cabinet door, and start the pulsed strong light treatment;

[0048] 1.3 Germin...