A kind of flavonoid ta31b and its preparation method and use

Abstract

The invention belongs to the field of medicine, and particularly relates to a flavonoid compound TA31b and a preparation method and application thereof. The structural formula of the compound is shown as a formula 1, the compound is named as 5-methoxy-6,4'-dyhydroxy flavonoid7-C-pyran glucosyl-4'-O-(4'''-pyran glucosyl)glucopyranoside and is yellow powder, and the chemical formula is C34H42O20. The preparation method of the compound is characterized by comprising the steps that pennycress seeds are taken as raw materials and then processed through extraction with extract, extraction with organic solvent, chromatography with a reverse-phase silica gel column and separation and purification with high performance liquid chromatography. The compound has the specific antioxidant activity, antineoplastic activity and immunological enhancement activity and the PC12 cell protection effect, and a new choice is supplied to clinical application.

Description

technical field

[0001] The invention belongs to the field of medicine, and in particular relates to a flavonoid compound TA31b and its preparation method and application. Background technique

[0002] Grucodon is a commonly used drug in Tibetan medicine, which belongs to the dried seeds of Grucolum, a plant of the genus Thlaspi arvense L. in the family Brassicaceae. According to the records of traditional medicine classics "Shen Nong's Materia Medica" and "Chinese Materia Medica", the seeds of scorpion are pungent and slightly warm. Mainly improving eyesight, tearing out of eye pain, benefiting eyesight, nourishing the five internal organs, eliminating numbness, dispelling rheumatism, mainly treating conjunctival congestion, swelling and pain, wind-flowing tears, rheumatic arthralgia, etc. The classic Tibetan medicine book "Jingzhu Materia Medica" records that the seeds of 菥蓂子 have the effect of clearing kidney heat and lung heat, and treating various kidney diseases. It is...

Examples

Embodiment Construction

[0071] A flavonoid compound of the present invention is obtained by extracting, separating and purifying from Fructus Grucoides by the following method:

[0072]Take 10kg of scorpion seeds, crush them to 20-40 mesh, add 10 times the volume of 95% ethanol to reflux and extract 3 times, each time for 60min, combine the extracts, filter, and concentrate under reduced pressure to obtain 1.57kg of extract, which is mixed with distilled water Add an equal volume of petroleum ether (60°C-90°C) to the separatory funnel to extract, shake fully, and collect the aqueous phase solution after the mixed liquid is separated. After repeated operations for 5 times, add an equal volume of acetic acid to the aqueous phase solution Ethyl ester extraction, fully oscillated and mixed, after the mixed solution was clarified and stratified, collected the aqueous phase solution, after repeated operations 5 times, the aqueous phase solution was extracted by adding an equal volume of n-butanol, fully osc...

technical field

[0001] The invention belongs to the field of medicine, and in particular relates to a flavonoid compound TA31b and its preparation method and application. Background technique

[0002] Grucodon is a commonly used drug in Tibetan medicine, which belongs to the dried seeds of Grucolum, a plant of the genus Thlaspi arvense L. in the family Brassicaceae. According to the records of traditional medicine classics "Shen Nong's Materia Medica" and "Chinese Materia Medica", the seeds of scorpion are pungent and slightly warm. Mainly improving eyesight, tearing out of eye pain, benefiting eyesight, nourishing the five internal organs, eliminating numbness, dispelling rheumatism, mainly treating conjunctival congestion, swelling and pain, wind-flowing tears, rheumatic arthralgia, etc. The classic Tibetan medicine book "Jingzhu Materia Medica" records that the seeds of 菥蓂子 have the effect of clearing kidney heat and lung heat, and treating various kidney diseases. It is...

Examples

Embodiment Construction

[0071] A flavonoid compound of the present invention is obtained by extracting, separating and purifying from Fructus Grucoides by the following method:

[0072]Take 10kg of scorpion seeds, crush them to 20-40 mesh, add 10 times the volume of 95% ethanol to reflux and extract 3 times, each time for 60min, combine the extracts, filter, and concentrate under reduced pressure to obtain 1.57kg of extract, which is mixed with distilled water Add an equal volume of petroleum ether (60°C-90°C) to the separatory funnel to extract, shake fully, and collect the aqueous phase solution after the mixed liquid is separated. After repeated operations for 5 times, add an equal volume of acetic acid to the aqueous phase solution Ethyl ester extraction, fully oscillated and mixed, after the mixed solution was clarified and stratified, collected the aqueous phase solution, after repeated operations 5 times, the aqueous phase solution was extracted by adding an equal volume of n-butanol, fully osc...

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