Solid-phase competition ELISA reagent kit of antibody for O-typed foot and mouth disease virus based on specific monoclonal antibody

A foot-and-mouth disease virus and solid-phase competition technology, which is applied in the field of O-type foot-and-mouth disease virus antibody solid-phase competition ELISA kit, can solve the problems of not being very precise, tedious and time-consuming, and multi-step procedures, so as to shorten the detection time and simplify ELISA Method steps, effect of improving stability

CN106405092AInactive Publication Date: 2017-02-15LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a solid-phase competition ELISA reagent kit of an antibody for an O-typed foot and mouth disease virus based on a specific monoclonal antibody, which comprises an O-typed foot and mouth disease specific monoclonal antibody marked by HRP (hypothalamic regulatory peptides). The reagent kit of the invention can be used for monitoring a level of the antibody for the O-typed foot and mouth disease virus. By replacing a rabbit antibody, guinea pig antiserum and guinea pig enzyme mark antibody in existing ELISA, the invention simplifies the steps of the ELISA method, improves stability, and greatly shorten detection time from 24 h to 1.5 h or less, thereby breaking through reaction processes for a primary antibody and a second antibody required by original ELISA.
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Description

technical field

[0001] The invention relates to the field of biological kits, in particular to a type O foot-and-mouth disease virus antibody solid-phase competition ELISA kit based on a type-specific monoclonal antibody. Background technique

[0002] Foot-and-mouth disease is an acute, febrile and contagious infectious disease common to cloven-hoofed animals caused by foot-and-mouth disease virus, which will cause huge economic losses to animal husbandry production and import and export trade. Human health poses a great risk. As a large cattle and sheep breeding country in China, foot-and-mouth disease is an extremely important disease that endangers cattle and sheep breeding. Strengthening the research and development of new technologies for the diagnosis and detection of foot-and-mouth disease and breaking through the key technologies and processes for the industrialization of foot-and-mouth disease diagnostic reagents is a solution to the rapid development of animal husb...

Examples

Embodiment 1

[0028] 1.1 Materials

[0029] 1.1.1 Experimental animals, antigens and cells

[0030] 8-week-old SPF BALB / c female mice were purchased from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences; SP / 20 and BHK-21 cells were purchased from the National Collection Center of Wuhan Institute of Virology, Chinese Academy of Sciences. Type O FMD virus inactivated antigen, A type FMD virus inactivated antigen, and Asia1 type FMD virus inactivated antigen were purchased from Zhongnong Weite Biotechnology Co., Ltd.

[0031] 1.1.2 Main reagents

[0032] Liquid paraffin, goat anti-mouse IgG-HRP was purchased from Sigma Company, 96-well microtiter plate was purchased from Costar Company, 2mol / L H2SO4 4 Provided by the detection team of Lanzhou Veterinary Research Institute, fetal bovine serum was purchased from GIBICO Company, TMB was purchased from SurModics Company, improved RPMI-1640 was purchased from Hyclone Company, carbonate buffer capsules were purchased...