A composition for improving the ability of coccidia oocysts to penetrate cells and its preparation method

Abstract

The invention relates to a composition capable of improving the cell-penetrating capacity of coccidian oocysts and a preparation method of the composition. The composition is prepared from the following components in parts by weight: 5-30 parts of herba houttuyniae extract, 5-30 parts of rheum officinale extract, 0.1-0.5 part of guar gum hydroxypropyl trimethyl ammonium chloride, 5-30 parts of lysozyme, and 10-90 parts of sterile water. For the composition, the prescription is scientific and reasonable, the process is simple, the use is convenient, and the cost is low; for the composition, the functions of breaking the structure of the cell walls, increasing the force for penetrating the cell membranes, and reducing the resistance of cell sap are utilized for increasing the number of sporozoites in the oocyst which enter the intestinal epithelial cells, so that the sporozoites can be rapidly fixed and breed in the intestinal cells.

Description

technical field

[0001] The invention belongs to the field of veterinary biological products, in particular to a composition for improving the ability of coccidia oocysts to penetrate cells and a preparation method thereof. Background technique

[0002] At present, domestic breeding tends to be intensive and large-scale. According to national food safety regulations, some antibiotics and anti-parasitic drugs are prohibited from being added to feed, and drug residues are also strictly controlled. The coccidiosis basically relies on the coccidia vaccine to prevent and control the parasite. According to the clinical results, the immune effect of the common coccidia vaccine is not ideal. The coccidia oocysts rupture the oocyst wall and release Sporozoites, these sporozoites need to breathe, and the carbohydrates stored in the body will drop in a short time, and if they cannot quickly enter the cells, they will lose their vitality, especially in the hot summer when the coccidia ar...

Examples

Embodiment 1

[0022] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protection solution are: 5 kg of Houttuynia cordata extract, 5 kg of rhubarb extract, and hydroxypropyl trimethyl chloride of guar gum Ammonium 0.1kg, lysozyme 5kg, sterilized water 10kg.

[0023] A composition for improving the ability of coccidia oocysts to penetrate cells and a preparation method thereof, the steps of the preparation method are:

[0024] (1) Weigh Houttuynia cordata extract, rhubarb extract, guar gum hydroxypropyltrimethylammonium chloride, and lysozyme in sequence according to the weight ratio, and then add sterilized water until submerged After soaking the above-mentioned ingredients at room temperature for 1 hour, continue to add the remaining sterilized water and stir evenly until the ingredients are completely dissolved, then filter with a 0.45um polyethersulfone filter element to obtain solution A, and store it in the liquid mixing ...

Embodiment 2

[0029] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protective solution are: Houttuynia cordata extract 30kg, rhubarb extract 30kg, guar gum hydroxypropyl trimethyl chloride Ammonium 0.5kg, lysozyme 30kg, sterilized water 90kg.

[0030] The steps of its preparation method are the same as in Example 1.

Embodiment 3

[0032] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protective solution are: Houttuynia cordata extract 15kg, rhubarb extract 15kg, guar gum hydroxypropyl trimethyl chloride Ammonium 0.3kg, lysozyme 15kg, sterilized water 65kg.

[0033] The steps of its preparation method are the same as in Example 1.

[0034] Clinical application

[0035] Test description:

[0036] In March 2015, a clinical comparative test was carried out in Qinhuangdao Zhengda Group Breeding Company. 300 7-day-old chickens were taken for the test, with 100 chickens in each group. They were the blank group, the vaccine immunization group sold on the market, and the group using the present invention. Three groups All are normally fed with sterilized water and non-antibiotic feed respectively; the immunization vaccine uses the Guangdong Foshan canon coccidiosis vaccine, 1000 feathers / bottle, 400 ± 10 parts / feather, and the chickens are used...

technical field

[0001] The invention belongs to the field of veterinary biological products, in particular to a composition for improving the ability of coccidia oocysts to penetrate cells and a preparation method thereof. Background technique

[0002] At present, domestic breeding tends to be intensive and large-scale. According to national food safety regulations, some antibiotics and anti-parasitic drugs are prohibited from being added to feed, and drug residues are also strictly controlled. The coccidiosis basically relies on the coccidia vaccine to prevent and control the parasite. According to the clinical results, the immune effect of the common coccidia vaccine is not ideal. The coccidia oocysts rupture the oocyst wall and release Sporozoites, these sporozoites need to breathe, and the carbohydrates stored in the body will drop in a short time, and if they cannot quickly enter the cells, they will lose their vitality, especially in the hot summer when the coccidia ar...

Examples

Embodiment 1

[0022] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protection solution are: 5 kg of Houttuynia cordata extract, 5 kg of rhubarb extract, and hydroxypropyl trimethyl chloride of guar gum Ammonium 0.1kg, lysozyme 5kg, sterilized water 10kg.

[0023] A composition for improving the ability of coccidia oocysts to penetrate cells and a preparation method thereof, the steps of the preparation method are:

[0024] (1) Weigh Houttuynia cordata extract, rhubarb extract, guar gum hydroxypropyltrimethylammonium chloride, and lysozyme in sequence according to the weight ratio, and then add sterilized water until submerged After soaking the above-mentioned ingredients at room temperature for 1 hour, continue to add the remaining sterilized water and stir evenly until the ingredients are completely dissolved, then filter with a 0.45um polyethersulfone filter element to obtain solution A, and store it in the liquid mixing ...

Embodiment 2

[0029] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protective solution are: Houttuynia cordata extract 30kg, rhubarb extract 30kg, guar gum hydroxypropyl trimethyl chloride Ammonium 0.5kg, lysozyme 30kg, sterilized water 90kg.

[0030] The steps of its preparation method are the same as in Example 1.

Embodiment 3

[0032] A composition for improving the ability of coccidia oocysts to penetrate cells. The components and weights of the protective solution are: Houttuynia cordata extract 15kg, rhubarb extract 15kg, guar gum hydroxypropyl trimethyl chloride Ammonium 0.3kg, lysozyme 15kg, sterilized water 65kg.

[0033] The steps of its preparation method are the same as in Example 1.

[0034] Clinical application

[0035] Test description:

[0036] In March 2015, a clinical comparative test was carried out in Qinhuangdao Zhengda Group Breeding Company. 300 7-day-old chickens were taken for the test, with 100 chickens in each group. They were the blank group, the vaccine immunization group sold on the market, and the group using the present invention. Three groups All are normally fed with sterilized water and non-antibiotic feed respectively; the immunization vaccine uses the Guangdong Foshan canon coccidiosis vaccine, 1000 feathers / bottle, 400 ± 10 parts / feather, and the chickens are used...

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