Shiitake mother culture medium preparation method and shiitake mother culture medium

Abstract

The invention relates to a shiitake mother culture medium preparation method and a shiitake mother culture medium, and belongs to the technical field of culture medium preparation. The shiitake mother culture medium is obtained through adding a filter juice obtained after cooking fine wood chip and wheat bran and a cellulose natural culture medium suitable for the growth of shiitake mycelia to a conventional PDA culture medium, and has the advantages of effective improvement of the cellulose and lignin decomposition ability of the shiitake mycelia, effective prevention of the degeneration of shiitake mother culture and many branches of the tips of the mycelia, certain rejuvenation effect on the shiitake mother culture, and facilitation of the keeping of the original mother culture characteristics of the shiitake. When shiitake mother culture medium is used to make the mother culture, the mycelia grow vigorously, are white and dense, and have the advantages of fast growth speed, strong cellulose decomposition ability and high disease resistance after inoculation. The success rate of mother culture inoculation reaches 99% or more, so the preparation method and the shiitake mother culture medium are suitable for being promoted.

Description

technical field

[0001] The invention relates to a method for preparing a culture medium for female shiitake mushrooms and a culture medium for female mushrooms, belonging to the technical field of culture medium preparation. Background technique

[0002] The slope preservation method is a commonly used strain preservation method at present. This method is simple and easy, does not require special equipment, and can observe the mycelium growth of the preserved strain in real time. The mother species preserved by this method are generally preserved by conventional PDA medium.

[0003] However, the PDA medium belongs to the semi-synthetic agar medium. In this medium, although the mushroom hyphae can carry out normal vegetative growth, they cannot be used for fruiting. Over time, the inherent reproductive growth factors of shiitake mushrooms cannot be activated. At the same time, many extracellular enzymes in shiitake mushrooms, such as cellulolytic enzymes, hemicellulolytic en...

Examples

Embodiment 1

[0026] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0027] 1) A, take 95g of broad-leaved forest fine sawdust and 5g of bran, wrap them with four layers of gauze respectively, place them in 500mL of water and boil for 30min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0028] B. Take 160g of peeled potatoes, put them in 500mL of water and boil for 30 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 2

[0032] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0033] 1) A, take 90g of broad-leaved forest fine sawdust and 7g of bran, wrap them with four layers of gauze respectively, place them in 450mL water and boil for 20min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0034] B. Take 150g of peeled potatoes, put them in 450mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 8 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 3

[0038] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0039] 1) A, take 100g of broad-leaved forest fine sawdust and 3g of bran, wrap them with four layers of gauze respectively, place them in 450mL of water and boil for 25min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0040] B. Take 170g of peeled potatoes, put them in 450mL of water and boil for 25min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 12g of glucose and 17g of agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45min.

[0042] The medium of mushroom female species in this example was prepared by the above method.

technical field

[0001] The invention relates to a method for preparing a culture medium for female shiitake mushrooms and a culture medium for female mushrooms, belonging to the technical field of culture medium preparation. Background technique

[0002] The slope preservation method is a commonly used strain preservation method at present. This method is simple and easy, does not require special equipment, and can observe the mycelium growth of the preserved strain in real time. The mother species preserved by this method are generally preserved by conventional PDA medium.

[0003] However, the PDA medium belongs to the semi-synthetic agar medium. In this medium, although the mushroom hyphae can carry out normal vegetative growth, they cannot be used for fruiting. Over time, the inherent reproductive growth factors of shiitake mushrooms cannot be activated. At the same time, many extracellular enzymes in shiitake mushrooms, such as cellulolytic enzymes, hemicellulolytic en...

Examples

Embodiment 1

[0026] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0027] 1) A, take 95g of broad-leaved forest fine sawdust and 5g of bran, wrap them with four layers of gauze respectively, place them in 500mL of water and boil for 30min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0028] B. Take 160g of peeled potatoes, put them in 500mL of water and boil for 30 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 2

[0032] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0033] 1) A, take 90g of broad-leaved forest fine sawdust and 7g of bran, wrap them with four layers of gauze respectively, place them in 450mL water and boil for 20min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0034] B. Take 150g of peeled potatoes, put them in 450mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 8 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 3

[0038] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0039] 1) A, take 100g of broad-leaved forest fine sawdust and 3g of bran, wrap them with four layers of gauze respectively, place them in 450mL of water and boil for 25min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0040] B. Take 170g of peeled potatoes, put them in 450mL of water and boil for 25min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 12g of glucose and 17g of agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45min.

[0042] The medium of mushroom female species in this example was prepared by the above method.

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