A Method for Quickly Identifying Drought Resistance of Wheat Using Leaf Regreening Ratio

Abstract

The invention discloses a method for quickly identifying the drought resistance of wheat by using the green recovering rate of leaves. Drought stress treatment is performed in a three-leaf period of wheat, the time from dryness to green recovering of the leaves and the ratio of the leaves which recover green are detected after rehydration, and the drought resistance of the wheat can be identifiedquickly through comparison and rating; the result of drought resistance identification is in good accordance with the result of a method for identifying drought resistance indexes. The method has theadvantages of rapidity, high efficiency, low cost, easy operation and the like, field water management of farmers can be guided during production, and through the cooperation with other drought resistance identifying methods in scientific researches, excellent drought-resistant genetic resources can be selected quickly in a batch of materials.

Description

technical field

[0001] The invention relates to the field of drought resistance identification of wheat, in particular to a method for rapidly identifying drought resistance of wheat by using the leaf regreening ratio. Background technique

[0002] Wheat is an important food crop. In northern my country, most of wheat is under drought stress throughout its life. Therefore, the research on wheat drought resistance identification is of great significance to ensure national food security and promote sustainable agricultural development. In scientific research and practice, the breeding of drought-resistant wheat varieties is a key research direction. The mechanism of plants adapting to drought can be divided into three categories: drought avoidance, drought resistance and drought tolerance, among which drought resistance and drought tolerance are collectively referred to as drought resistance. The identification results of drought resistance of wheat are different in academic ...

Examples

Embodiment 1

[0026] Example 1: Identify the drought resistance of 12 wheat varieties in Shanxi Province

[0027] The test materials were 12 wheat germplasms with different drought resistance indices provided by Guzi, Shanxi Academy of Agricultural Sciences. They were carried out in the greenhouse of the Institute of Genetics, Hebei Academy of Agriculture and Forestry Sciences. Xifeng 20 was used as a control variety, which is a local variety with better drought resistance.

[0028] The loam and vermiculite were evenly mixed at a ratio of 2:1 and placed in a pot with a soil depth of 15 cm. After that, 30 well-selected germinated seeds were evenly planted in the culture medium to a depth of 4 cm, and then sprayed with Haogland nutrient solution to adjust the field relative water holding capacity of the soil to 75%. The space is kept ventilated and the relative humidity of the air is 50%. Afterwards, spray 3-times diluted Haogland nutrient solution 3-6 times a day to keep the field relative ...

Embodiment 2

[0030] Example 2: Identification of drought resistance of 23 wheat varieties in Hebei Province

[0031] The test materials were 23 wheat varieties with different drought resistance indices provided by the Hebei Academy of Agricultural Sciences Dry Farming Institute. The experiment was carried out in the rainproof shed (outdoor) of the Hebei Academy of Agricultural Sciences, and no control varieties were set.

[0032] The loam and vermiculite were evenly mixed at a ratio of 2:1 and placed in a pot with a soil depth of 20 cm. Afterwards, 30 well-selected germinated seeds were evenly planted in the culture medium to a depth of 4 cm, and then the Haogland nutrient solution was used to adjust the field relative water holding capacity of the soil to 75%. Afterwards, tap water is sprayed 4-6 times every day to keep the relative water holding capacity of the soil at about 80%. When the leaf age is 2.2, gradually control the field relative water holding capacity of the soil to about 6...

technical field

[0001] The invention relates to the field of drought resistance identification of wheat, in particular to a method for rapidly identifying drought resistance of wheat by using the leaf regreening ratio. Background technique

[0002] Wheat is an important food crop. In northern my country, most of wheat is under drought stress throughout its life. Therefore, the research on wheat drought resistance identification is of great significance to ensure national food security and promote sustainable agricultural development. In scientific research and practice, the breeding of drought-resistant wheat varieties is a key research direction. The mechanism of plants adapting to drought can be divided into three categories: drought avoidance, drought resistance and drought tolerance, among which drought resistance and drought tolerance are collectively referred to as drought resistance. The identification results of drought resistance of wheat are different in academic ...

Examples

Embodiment 1

[0026] Example 1: Identify the drought resistance of 12 wheat varieties in Shanxi Province

[0027] The test materials were 12 wheat germplasms with different drought resistance indices provided by Guzi, Shanxi Academy of Agricultural Sciences. They were carried out in the greenhouse of the Institute of Genetics, Hebei Academy of Agriculture and Forestry Sciences. Xifeng 20 was used as a control variety, which is a local variety with better drought resistance.

[0028] The loam and vermiculite were evenly mixed at a ratio of 2:1 and placed in a pot with a soil depth of 15 cm. After that, 30 well-selected germinated seeds were evenly planted in the culture medium to a depth of 4 cm, and then sprayed with Haogland nutrient solution to adjust the field relative water holding capacity of the soil to 75%. The space is kept ventilated and the relative humidity of the air is 50%. Afterwards, spray 3-times diluted Haogland nutrient solution 3-6 times a day to keep the field relative ...

Embodiment 2

[0030] Example 2: Identification of drought resistance of 23 wheat varieties in Hebei Province

[0031] The test materials were 23 wheat varieties with different drought resistance indices provided by the Hebei Academy of Agricultural Sciences Dry Farming Institute. The experiment was carried out in the rainproof shed (outdoor) of the Hebei Academy of Agricultural Sciences, and no control varieties were set.

[0032] The loam and vermiculite were evenly mixed at a ratio of 2:1 and placed in a pot with a soil depth of 20 cm. Afterwards, 30 well-selected germinated seeds were evenly planted in the culture medium to a depth of 4 cm, and then the Haogland nutrient solution was used to adjust the field relative water holding capacity of the soil to 75%. Afterwards, tap water is sprayed 4-6 times every day to keep the relative water holding capacity of the soil at about 80%. When the leaf age is 2.2, gradually control the field relative water holding capacity of the soil to about 6...

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