Chromatographic device and working method thereof

Abstract

The present invention provides a chromatography device and an operating method thereof. The chromatography device includes a base, an upper cover, a heating component, and a temperature measuring component; two fixing units, each fixed to the base and respectively securing two ends of a chromatography column by compression; and a accommodating unit, comprising: a first blocking portion fixed to the base and extending vertically; a second blocking portion disposed on the base peripherally of the first blocking portion and extending vertically; and a third blocking portion disposed on an upper portion of the base and extending from the second blocking portion toward the first blocking portion. The fixing unit and the accommodating unit are located within a space enclosed by the base and the upper cover. The present invention has advantages such as uniform heating, simple operation, and good detection parallelism.

Description

Technical Field

[0001] The present invention relates to chromatography, in particular to a chromatography device with a heated chromatography column and a working method thereof. Background Art

[0002] Nano ESI column heating can significantly improve the sensitivity and signal-to-noise ratio of organic analysis, and has wide application and scientific research value.

[0003] Existing chromatographic columns are all coated quartz capillaries with excellent toughness, allowing them to be freely wound like electrical wires. However, their heat tracing devices cannot guarantee the consistency of the operating environment in terms of thermal insulation, and the heating structure cannot provide constant and uniform heat. This requires high operability, resulting in high technical requirements and expensive replacement costs. For example, the existing chromatographic column heating system (also called chromatographic column oven) of the thermoelectric Nano ESI requires the original standard Nano ESI to be completely removed and replaced with a new Nano ESI with heating. Furthermore, researchers in research institutes and companies are accustomed to custom-made, open-type Nano ESIs, which facilitate the adjustment and better control of Nano ESI. This makes the existing devices unable to meet technical requirements, especially for proteomics experts. Summary of the Invention

[0004] In order to solve the deficiencies in the above-mentioned prior art solutions, the present invention provides a chromatography device with uniform heating and simple operation.

[0005] The purpose of the present invention is achieved through the following technical solutions:

[0006] A chromatographic device, comprising a base, an upper cover, a heating component, and a temperature measuring component; the chromatographic device further comprises:

[0007] Two fixing units, the fixing units are fixed on the base and respectively fix the two ends of the chromatographic column by pressing;

[0008] A receiving unit, comprising:

[0009] a first blocking portion, which is fixed to the base and extends in a vertical direction;

[0010] a second blocking portion, the second blocking portion being arranged on a base outside the first blocking portion and extending in a vertical direction;

[0011] a third blocking portion, the third blocking portion being disposed on an upper portion of the base and extending from the second blocking portion toward the first blocking portion;

[0012] The fixing unit and the accommodating unit are located in a space surrounded by the base and the upper cover.

[0013] The present invention also aims to provide a method for operating the above-mentioned chromatographic device that is easy to operate, and this object is achieved through the following technical solutions:

[0014] According to the above-mentioned working method of the chromatographic device, the working method of the chromatographic device comprises the following steps:

[0015] (A1) fixing one end of the chromatography column using the fixing unit;

[0016] (A2) Pulling the connecting portion upward, the protrusion moves relatively in the hole, the second blocking portion moves upward, and the distance between the bottom end of the second blocking portion and the base is greater than zero;

[0017] (A3) wrapping the chromatographic column around the periphery of the first barrier portion at the lower side of the bottom end of the second barrier portion, wherein the diameter of the chromatographic column around the first barrier portion is no larger than the diameter of the area enclosed by the second barrier portion;

[0018] (A4) pushing the connecting portion downward, the connecting member fixes the connecting portion and the first blocking portion, and the chromatographic column surrounding the first blocking portion is stuck between the first blocking portion and the second blocking portion, with the upper portion blocked by the protrusion;

[0019] (A5) Fix the other end of the chromatography column using another fixing unit.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. Can be used on the standard nano ESI, greatly reducing customer costs;

[0022] 2. Universal chromatographic columns can be used, and can adapt to chromatographic columns of various lengths and sizes, greatly reducing customer use costs;

[0023] 3. Simple structure, wide application range and high degree of personalization;

[0024] 4. Good temperature control effect and wide range. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The disclosure of the present invention will become more easily understood with reference to the accompanying drawings. Those skilled in the art will readily appreciate that these drawings are merely used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention.

[0026] Figure 1 is a simplified structural diagram of a chromatographic device according to Example 1 of the present invention;

[0027] Figure 2 is a simplified structural diagram of a fixing unit according to an embodiment of the present invention;

[0028] Figure 3is a simplified structural diagram of a receiving unit according to embodiment 1 of the present invention;

[0029] Figure 4 is a simplified structural diagram of a receiving unit according to embodiment 2 of the present invention;

[0030] Figure 5 2 is a simplified cross-sectional view of a housing unit according to embodiment 2 of the present invention. DETAILED DESCRIPTION

[0031] Figure 1-5 The following description describes alternative embodiments of the present invention to teach those skilled in the art how to implement and reproduce the present invention. In order to teach the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will understand that variations or substitutions derived from these embodiments will be within the scope of the present invention. Those skilled in the art will understand that the following features can be combined in various ways to form multiple variations of the present invention. Thus, the present invention is not limited to the following alternative embodiments, but is limited only by the claims and their equivalents.

[0032] Example 1:

[0033] Figure 1 The structure diagram of the chromatographic device of Example 1 of the present invention is schematically shown as follows: Figure 1 As shown, the chromatographic device comprises:

[0034] Base 2, upper cover 1, cross-connection 7, heating component and temperature measuring component; the upper cover 1 is fixed to the base 2, thereby enclosing a space; the base 2 has a blind hole 21, and the heating component and temperature measuring component are arranged in the blind hole 21. Through the operation of the heating component and the temperature measuring component, the air in the space is maintained at a set temperature;

[0035] Figure 2 A schematic structural diagram of a fixing unit according to an embodiment of the present invention is shown as follows: Figure 2 As shown, there are two fixing units, the fixing unit 3 is fixed on the base 2, and the two ends of the chromatographic column are fixed by pressing; the specific structure is:

[0036] A base 31 having two oppositely disposed grooves 312, such as V-shaped grooves;

[0037] A rotating arm 34, one end of which is disposed on the base 31 via a rotating shaft 33; when the rotating arm 34 rotates, it is stuck between the two grooves 312;

[0038] A first connecting member, the first connecting member is used to connect the other end of the rotating arm and the base, such as using magnets 311 and 341 to fix the rotating arm 34 and the base 31;

[0039] Figure 3 A schematic structural diagram of the accommodation unit according to an embodiment of the present invention is shown as follows: Figure 3 As shown, the accommodating unit 4 includes:

[0040] A first blocking portion 41 is fixed to the base 2 and extends in the vertical direction. In this embodiment, the first blocking portion 41 is the only cylindrical component suitable for surrounding the chromatographic column.

[0041] A second blocking portion 42 is provided on the base 2 outside the first blocking portion 41 and extends in the vertical direction. The second blocking portion 42 of this embodiment is composed of multiple components extending in the vertical direction and provided outside the first blocking portion 41. Each component has a through hole 44 extending in the vertical direction.

[0042] The third blocking portion 43 is provided on the upper portion of the base 2 and extends from the second blocking portion 42 toward the first blocking portion 41; thereby constraining the chromatographic column within the space enclosed by the first blocking portion 41, the second blocking portion 42, and the third blocking portion 43, preventing the chromatographic column from spreading freely; the third blocking portion 43 in this embodiment is a plurality of protrusions provided on the outer edge of the first blocking portion 41, and the protrusions are located in the through holes 44 of the second blocking portion, allowing the second blocking portion to move up and down;

[0043] The fixing unit 3 and the accommodating unit 4 are located in the space enclosed by the base and the upper cover;

[0044] A connecting portion 45 , wherein the connecting portion 45 connects the second blocking portions 42 , such as a circular structure;

[0045] The second connecting member 46 is used to connect and fix the first blocking part 41 and the connecting part 45; this embodiment uses magnets, and the first magnet and the second magnet are respectively arranged on the connecting part and the first blocking part, and the connecting part is fixed to the first blocking part by utilizing the attraction between opposite poles of the magnets.

[0046] According to the working method of the chromatographic device in an embodiment of the present invention, the working method of the chromatographic device includes the following steps:

[0047] (A1) fixing one end of the chromatographic column using the fixing unit 3, specifically:

[0048] (B1) The chromatographic column is placed in two oppositely disposed grooves 312:

[0049] (B2) The rotating arm 34 rotates around the rotating shaft 33 to compress the chromatographic column between the two grooves 31234;

[0050] (B3) fixing the rotating arm 34 to the base 31, such as by magnet attraction, with the fixing point, the groove, and the rotating shaft arranged in sequence;

[0051] (A2) Pull the connecting portion 45 upward, the first magnet and the second magnet separate, the protrusion moves relatively in the hole 44, the second blocking portion 42 moves upward, and the distance between the bottom end of the second blocking portion 42 and the base 2 is greater than zero;

[0052] (A3) wrapping the chromatographic column around the periphery of the first barrier portion 41 at the lower side of the bottom end of the second barrier portion 42, wherein the diameter of the chromatographic column around the first barrier portion 41 is no larger than the diameter of the area enclosed by the second barrier portion 42;

[0053] (A4) Pushing the connecting portion 45 downward, the second connecting member fixes the connecting portion 45 and the first blocking portion 41. The chromatographic column surrounding the first blocking portion 41 is stuck between the first blocking portion 41 and the second blocking portion 42, and the upper portion is blocked by the protrusion. That is, the surrounding chromatographic column is blocked in the space enclosed by the first blocking portion, the second blocking portion, and the third blocking portion.

[0054] (A5) Using another fixing unit 3 to fix the other end of the chromatographic column, the end of the chromatographic column is connected to the cross-connection 7; the specific method of fixing is:

[0055] (B1) The chromatographic column is placed in two oppositely disposed grooves 312:

[0056] (B2) The rotating arm 34 rotates around the rotating shaft 33 to compress the chromatographic column between the two grooves 31234;

[0057] (B3) Fix the rotating arm 34 to the base 31, such as by magnet attraction, with the fixing point, groove and rotating shaft arranged in sequence.

[0058] Example 2:

[0059] The chromatographic device of embodiment 2 of the present invention comprises:

[0060] A base, an upper cover, a heating component, and a temperature measuring component; the upper cover is fixed to the base, thereby enclosing a space; the base has a blind hole, the heating component and the temperature measuring component are arranged in the blind hole, and the operation of the heating component and the temperature measuring component maintains the air in the space at a set temperature;

[0061] Figure 2 A schematic structural diagram of a fixing unit according to an embodiment of the present invention is shown as follows: Figure 2 As shown, there are two fixing units, the fixing unit 3 is fixed on the base 2, and the two ends of the chromatographic column are fixed by pressing; the specific structure is:

[0062] A base 31 having two oppositely disposed grooves 312, such as V-shaped grooves;

[0063] A rotating arm 34, one end of which is disposed on the base 31 via a rotating shaft 33; when the rotating arm 34 rotates, it is stuck between the two grooves 312;

[0064] A first connecting member, the first connecting member is used to connect the other end of the rotating arm and the base, such as using magnets 311 and 341 to fix the rotating arm 34 and the base 31;

[0065] Figure 4-5 A schematic structural diagram of the accommodation unit according to an embodiment of the present invention is shown as follows: Figure 4-5 As shown, the accommodating unit includes:

[0066] A first blocking portion 41 is fixed to the base 2 and extends in the vertical direction. In this embodiment, the first blocking portion 41 is a sheet-like component that encloses four cylindrical spaces and is suitable for surrounding the chromatographic column.

[0067] The second blocking portion 42 is provided on the base 2 outside the first blocking portion 41 and extends in the vertical direction. The second blocking portion of this embodiment is a plurality of sheet-like components extending in the vertical direction and is provided outside the first blocking portion.

[0068] A third blocking portion is provided on the upper portion of the base and extends from the second blocking portion toward the first blocking portion; thereby constraining the chromatographic column within the space enclosed by the first blocking portion, the second blocking portion, and the third blocking portion to prevent the chromatographic column from spreading out at will; the number of the multiple third blocking portions in this embodiment is the same as that of the second blocking portion, and the third blocking portion includes a horizontal portion 431 and a vertical portion 432, one end of the horizontal portion 431 is connected to the second blocking portion 42, and the other end is connected to the vertical portion 432, and the vertical portion 432 is provided between the first blocking portion 41 and the second blocking portion 42; the area enclosed by the projection of the first blocking portion 41 on the base 2 and the area enclosed by the projection of the third blocking portion on the base do not intersect;

[0069] The fixing unit and the accommodating unit are located in a space surrounded by the base and the upper cover.

[0070] According to the working method of the chromatographic device in an embodiment of the present invention, the working method of the chromatographic device includes the following steps:

[0071] (A1) fixing one end of the chromatographic column using the fixing unit, specifically:

[0072] (B1) The chromatographic column is placed in two opposite grooves:

[0073] (B2) The rotating arm rotates around the axis to compress the chromatographic column between the two grooves;

[0074] (B3) fixing the rotating arm to the base, such as by magnet attraction, with the fixing point, the groove, and the rotating shaft arranged in sequence;

[0075] (A2) The chromatographic column is wrapped around the periphery of the first barrier 41 and is within the area enclosed by the third barrier (horizontal portion 431), that is, the diameter of the chromatographic column wrapped around the first barrier is no larger than the diameter of the area enclosed by the second barrier;

[0076] (A3) The chromatographic columns wrapped around the first blocking portion are pushed downward, causing them to spread out, i.e., their surrounding radius increases, but they are blocked by the second blocking portion 42 and their upper portion is blocked by the third blocking portion, i.e., the surrounding chromatographic columns are blocked within the space enclosed by the first, second, and third blocking portions;

[0077] (A5) Using another fixing unit to fix the other end of the chromatographic column; the specific fixing method is the same as step (A1).

Claims

1. A chromatographic device comprising a base, an upper cover, a heating component, and a temperature measuring component; characterized in that: The chromatographic device also includes: Two fixing units, the fixing units are fixed on the base and respectively fix the two ends of the chromatographic column by pressing; A receiving unit, comprising: a first blocking portion, which is fixed to the base and extends in a vertical direction; a second blocking portion, the second blocking portion being arranged on a base outside the first blocking portion and extending in a vertical direction; a third blocking portion, the third blocking portion being disposed on an upper portion of the base and extending from the second blocking portion toward the first blocking portion; The fixing unit and the accommodating unit are located in the space enclosed by the base and the upper cover; The third blocking portion is a plurality of protrusions arranged on the outer edge of the first blocking portion; The second blocking portion includes a plurality of components extending in a vertical direction, each component having a hole extending in a vertical direction; the protrusion is located in the hole and moves vertically relative to the components; a connecting portion, the connecting portion being used to connect to the second blocking portion; A connecting member is used to connect the connecting portion and the first blocking portion.

2. The chromatographic device according to claim 1, characterized in that: The first blocking portion includes only one cylindrical component, or includes a plurality of independent components enclosing a cylindrical space.

3. The chromatographic device according to claim 1, wherein: The connecting piece includes: a first magnet, the first magnet being disposed on top of the first blocking portion; A second magnet is provided on the connecting portion, and the first magnet and the second magnet attract each other.

4. The chromatographic device according to claim 1, wherein: The fixing unit includes: A base having two oppositely arranged grooves; A rotating arm, one end of which is disposed on the base via a rotating shaft; when the rotating arm rotates, it is stuck between the two grooves; A connecting piece is used to connect the other end of the rotating arm and the base.

5. The operating method of the chromatographic device according to claim 1, comprising the following steps: (A1) fixing one end of the chromatographic column using the fixing unit; (A2) Pulling the connecting portion upward, the protrusion moves relatively in the hole, the second blocking portion moves upward, and the distance between the bottom end of the second blocking portion and the base is greater than zero; (A3) wrapping the chromatographic column around the periphery of the first barrier portion at the lower side of the bottom end of the second barrier portion, wherein the diameter of the chromatographic column around the first barrier portion is no greater than the diameter of the area enclosed by the second barrier portion; (A4) Pushing the connecting portion downward, the connecting member fixes the connecting portion and the first blocking portion, and the chromatographic column surrounding the first blocking portion is stuck between the first blocking portion and the second blocking portion, with the upper portion blocked by the protrusion; (A5) Fix the other end of the chromatography column using another fixing unit.

6. The operating method of the chromatography device according to claim 5, characterized in that: The working method of the fixed unit includes the following steps: (B1) The chromatographic column is placed in two opposite grooves: (B2) The rotating arm rotates around the axis to compress the chromatographic column between the two grooves; (B3) Fix the rotating arm to the base, with the fixing point, groove and rotating shaft arranged in sequence.

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