A temperature-controlled microscopic system for epidemiological analysis and its operation method

Abstract

The embodiments of this specification disclose a temperature-controlled microscopic system for epidemiological analysis and its operation method, which at least include: an inverted microscope and a precision temperature-controlled slide; the precision temperature-controlled slide includes: a slide substrate, a temperature sensing element, a patterned heating layer, a protective layer, and a transparent protective cover; during the experiment, the temperature is flexibly and accurately controlled based on the patterned heating layer to observe the reaction of microorganisms in the sample liquid at different temperatures, and the sample liquid is protected by a transparent protective cover to prevent the sample liquid from being contaminated, and to avoid the spread of microorganisms in the sample liquid during the experiment and infection of the staff; in view of the presence of the transparent protective cover, the microorganisms in the sample liquid can be observed at close range based on an inverted microscope to achieve better observation effects. Furthermore, there is no need to repeatedly perform temperature treatment and sample preparation, and precise temperature control is performed during the observation process. The experimental means are flexible and diversified, and the experimental results are more accurate, avoiding the risk of pathogenic bacteria infection caused by repeated sample preparation.

Description

Technical Field

[0001] The present specification relates to the field of medical devices, and in particular to a temperature-controlled microscopy system for epidemiological analysis and an operating method thereof. Background Art

[0002] Epidemic refers to infectious diseases that can infect a large population. Infectious diseases that can spread widely in a short period of time, such as influenza, meningitis, cholera, etc. Microscopes are often used in epidemiological analysis to observe pathogenic microorganisms such as viruses and bacteria.

[0003] At present, in the field of pathological analysis, microscopes are generally used for testing experiments. For example, the reproduction process of microorganisms such as viruses and bacteria is observed through an inverted microscope to conduct treatment research.

[0004] However, the reproduction and cultivation of microorganisms requires a suitable environment, and currently, it can only be observed by using a microscope after culturing outside, which does not have the function of controlling the temperature of the culture solution, and thus cannot observe the reactions of microorganisms such as viruses and bacteria at different temperatures. In the art, there is an experimental method for observing the reproduction process of bacteria or viruses using an inverted microscope, but this method and its device are also unable to observe the reactions of pathogenic microorganisms at different temperatures, and due to the lack of effective isolation and protection, cases of scientific researchers being infected during the experiment are not uncommon. Summary of the invention

[0005] The purpose of the embodiments of this specification is to provide a temperature-controlled microscope system for epidemiological analysis and an operating method thereof, so as to effectively improve the accuracy, safety and flexibility of microbial observation experiments.

[0006] To solve the above technical problems, the embodiments of this specification are implemented as follows:

[0007] A temperature-controlled microscopy system for epidemiological analysis comprises at least: an inverted microscope and a precision temperature-controlled slide used with the inverted microscope; wherein:

[0008] The precise temperature-controlled glass slide comprises: a glass slide base, a temperature sensing element and a patterned heating layer located on a surface of the glass slide base and arranged in a sample detection area, a protective layer at least covering the patterned heating layer and the temperature sensing element, and a transparent protective cover located above the protective layer;

[0009] The surface of the glass slide base is also provided with a groove surrounding the sample detection area, the lower opening edge of the transparent protective cover is placed in the groove to at least cover the sample detection area therein, and disinfectant is dripped into the groove during sample detection;

[0010] The patterned heating layer extends through the groove to extend two first contacts at one end of the surface, outside the sample detection area. At the same time, the temperature sensing element also extends two second contacts at one end of the surface. The two first contacts are used to connect to the output end of the temperature control unit, and the two second contacts are used to connect to the input end of the temperature control unit, so as to control the sample liquid dripped in the sample detection area to reach a preset temperature according to user settings.

[0011] Optionally, it also includes: an electrical connection pressing block;

[0012] The lower surface of the electrical connection pressing block is provided with electrical connection bumps corresponding to the first contact and the second contact respectively, and the side surface of the electrical connection pressing block is provided with a microscope interface connected to the inverted microscope;

[0013] After the electrical connection pressing block is placed correspondingly on the glass slide substrate, the electrical connection bumps are in contact with the first contact and the second contact respectively; the patterned heating layer and the temperature sensing element are respectively connected to the temperature control unit integrated in the inverted microscope through the microscope interface.

[0014] Optionally, the patterned heating layer is made of ITO and / or metal.

[0015] Optionally, the patterned heating layer has a thickness ranging from 20 to 200 nm.

[0016] Optionally, the thickness of the temperature sensing element is equal to the thickness of the patterned heating layer.

[0017] Optionally, the material of the protective layer is an insulating material, and the thickness of the protective layer is in the range of 20-1000 nm.

[0018] Optionally, the electrical connection pressing block is filled with a weight material for maintaining balance.

[0019] Optionally, the microscope interface of the electrical connection block is a USB interface.

[0020] Through the technical solution of this specification, the temperature can be flexibly and accurately controlled based on the patterned heating layer set in the precise temperature-controlled slide to observe the reaction of microorganisms in the sample liquid at different temperatures, and the sample liquid can be protected by a transparent protective cover to prevent the sample liquid from being contaminated. At the same time, the spread of microorganisms in the sample liquid during the experiment is avoided to infect the staff; because the sample observation in epidemiological analysis may take several days, the failure of the disinfectant will put the scientific researchers at risk, and the setting of the reservoir makes it possible to replace the disinfectant regularly. The disinfectant in the reservoir is sucked out at a certain interval and replenished with new disinfectant. The new disinfectant in the reservoir will enter the groove again and continue to provide a protective belt to isolate viruses and bacteria; and, in view of the presence of the transparent protective cover, the microorganisms in the sample liquid can be observed at close range based on an inverted microscope to achieve better observation effects. Furthermore, from an overall analysis, there is no need to repeatedly perform temperature treatment and sample preparation, and precise temperature control can be performed during the observation process, making the experimental means more diverse, the experimental results more accurate, and avoiding the risk of pathogenic bacteria infection caused by repeated sample preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of this specification or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this specification. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0022] Figure 1 It is a scene schematic diagram of a temperature-controlled microscopy system for epidemiological analysis provided in an embodiment of this specification.

[0023] Figure 2 It is one of the structural schematic diagrams of the precise temperature-controlled slide provided in the embodiments of this specification.

[0024] Figure 3 This is the second structural schematic diagram of the precise temperature-controlled slide provided in the embodiments of this specification.

[0025] Figure 4 This is one of the structural schematic diagrams of the electrical connection pressing block provided in the embodiments of this specification.

[0026] Figure 5 This is the second structural schematic diagram of the electrical connection pressing block provided in the embodiment of this specification.

[0027] Figure 6 It is a schematic diagram of the structure of the temperature sensor element provided in the embodiment of this specification. DETAILED DESCRIPTION

[0028] In order to enable those skilled in the art to better understand the technical solutions in this specification, the technical solutions in the embodiments of this specification will be clearly and completely described below in conjunction with the drawings in the embodiments of this specification. Obviously, the described embodiments are only part of the embodiments of this specification, not all of the embodiments. Based on the embodiments in this specification, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of this specification.

[0029] Reference Figure 1 , which is a schematic diagram of a temperature-controlled microscope system for epidemiological analysis provided in an embodiment of the present specification, and the temperature-controlled microscope system at least includes: an inverted microscope 11, and a precision temperature-controlled glass slide 12 used in conjunction with the inverted microscope 11. It should be understood that a signal connection can be established between the inverted microscope 11 and the precision temperature-controlled glass slide 12, and the precision temperature-controlled glass slide 12 generally needs to be placed on the stage 111 of the inverted microscope 11 for use.

[0030] Reference Figure 2 and Figure 3 As shown, the precise temperature-controlled glass slide 12 further includes: a glass slide base 121 , a temperature sensing element 122 , a patterned heating layer 123 , a protective layer 124 and a transparent protective cover 125 .

[0031] in:

[0032] The material of the glass slide substrate 121 is generally a transparent substrate, for example, polymethyl methacrylate PMM, commonly known as organic glass; polystyrene PS; polycarbonate PC, etc.

[0033] The patterned heating layer 123 is located on any surface of the glass slide substrate 121 and is arranged in the sample detection area. The specific pattern thereof can be set according to the process capability and the experimental requirements. For example, it can be set as Figure 2 The wiring structure shown, wherein the gap between adjacent wires can be set to be the same as the wire width, or larger than the wire width, so as to avoid adjacent wires from contacting each other and causing a short circuit. In the embodiment of this specification, the material of the patterned heating layer 123 can be ITO and / or metal. In other words, the wires in the patterned heating layer 123 can all be transparent conductive ITO materials, or all be metal materials, or one section can be ITO materials and the remaining section can be metal materials.

[0034] The temperature sensing element 122 is also located on the same surface of the glass slide substrate 121 as the patterned heating layer 123, and the temperature sensing element 122 is arranged in the sample detection area. Specifically, the temperature sensing element 122 can be arranged in the pattern gap of the patterned heating layer 123, preferably, Figure 2 In this way, there is no mutual interference between the temperature sensing element 122 and the patterned heating layer 123, and the two can be led out independently without crossing. The temperature sensing element 122 is used to detect the temperature of the sample liquid; the temperature sensing element 122 can be an existing thin film temperature sensor, for example, refer to Figure 6 As shown, the thin film temperature sensing element at least includes: substrate 122-1, positive thermocouple film 122-2, negative thermocouple film 122-3, thermocouple junction 122-4, pad film 122-5, insulating transition film 122-6 and protective film 122-7, which has the effects of fast response time, small volume and accurate detection. In addition, other existing thin film temperature sensing elements can also be referred to.

[0035] The protection layer 124 at least covers the patterned heating layer 123 and the temperature sensing element 122 , thereby playing a role of isolation and insulation.

[0036] At the same time, the upper surface of the glass slide substrate 121 (i.e. Figure 2 The surface of the precision temperature-controlled glass slide 124 (where the patterned heating layer 123 and the temperature sensing element 122 are arranged) is also provided with a groove 126 surrounding the sample detection area, and a transparent protective cover 125 is arranged above the protective layer 124, and its lower opening edge is placed in the groove 126, so as to at least cover the sample detection area therein, that is, to cover the patterned heating layer 123 and the temperature sensing element 122 in the transparent protective cover 125. In this way, when using the precision temperature-controlled glass slide 12 for experimental observation, it is not necessary to open the transparent protective cover 125, and the sample liquid dripped on the precision temperature-controlled glass slide 12 can be observed using an inverted microscope. Thus, the transparent protective cover 125 can prevent the sample liquid from being contaminated and protect the purity of the sample liquid; at the same time, it can also prevent the spread of viruses or bacteria in the sample liquid and cause infection to scientific researchers or testers, especially when the sample liquid contains epidemic pathogens, it has a better protective effect. Preferably, during sample testing, disinfectant, for example, disinfectant 84 is dripped into the groove, so that the sample liquid can be fully isolated in the transparent protective cover 125 through the groove.

[0037] In a preferred embodiment, a liquid reservoir 127 is provided on the upper surface of the glass slide substrate 121, and a liquid conducting groove 129 connects the liquid reservoir 127 and the groove 126. The upper openings of the liquid reservoir 127 and the liquid conducting groove 129 are semi-enclosed by a flange 128 to allow the upper limit of the liquid level in the liquid reservoir 127 and / or the liquid conducting groove 129 to be slightly higher than the upper surface of the glass slide substrate 121; it should be understood that due to the influence of the surface tension of the liquid, the liquid surface in the liquid reservoir 127 and / or the liquid conducting groove 129 may not be a plane, and in the case where the liquid surface is convex, at least a portion of the liquid surface is slightly higher than the upper surface of the glass slide substrate 121.

[0038] It should be understood that when dropping the sample liquid, the sample liquid drop should be placed in the gap between the patterned heating layer 123 and the temperature sensing element 122, for example, in the central area of ​​the pattern formed by the patterned heating layer 123 and the temperature sensing element 122 (such as the area framed by the dotted line). The patterned heating layer 123 and the temperature sensing element 122 are not arranged in this area, and there will be no obstruction. Therefore, the strains in the sample liquid can be observed through the objective lens of the inverted microscope.

[0039] The reason for using an inverted microscope is that the objective lens of an ordinary microscope is at the top. Due to the presence of the transparent protective cover 125, the objective lens of an ordinary microscope cannot be close enough to the sample liquid, and it is also impossible to achieve a high magnification for accurate observation. The objective lens of an inverted microscope is at the bottom, and it can approach the sample liquid from a close distance from the bottom. At the same time, considering that the bacteria or viruses to be observed in the sample liquid are usually gathered at the bottom. Therefore, from an overall consideration, using an inverted microscope with the objective lens at the bottom can observe bacteria or viruses based on the closest distance, and can achieve a better observation effect.

[0040] It should be understood that in the present embodiment, the sample detection area may be the middle area of ​​the glass slide substrate 121. Specifically, its range may be approximately Figure 3 The area surrounded by the middle groove 126.

[0041] The patterned heating layer 123 passes through the groove 126 and extends two first contacts at one end of the surface and outside the sample detection area, for example Figure 3 As shown in FIG. 1 , first contacts ① and ② are extended from the right end of the surface of the glass slide substrate 121 , and at the same time, the temperature sensing element 122 also extends two second contacts from one end of the surface, for example Figure 3 As shown in the figure, second contacts ③ and ④ are extended from the same end of the glass slide substrate 121; wherein, the two first contacts ① and ② are used to connect to the output end of the temperature control unit (not shown), and the two second contacts ③ and ④ are used to connect to the input end of the temperature control unit, so as to control the sample liquid dripped in the sample detection area to reach a preset temperature according to the user's setting. The leads of the second contacts ③ and ④ of the temperature sensing element 122 can be metal or ITO, which is not limited in this specification. Accordingly, the first contacts ① and ②, and the second contacts ③ and ④ can be metal contacts or ITO contacts.

[0042] It should be understood that in this specification, the temperature control unit is not shown. It can be independently set and independently control the patterned heating layer 123, or it can be integrated in an inverted microscope, for example, to control the heating of the patterned heating layer 123 through the human-computer interaction interface of the inverted microscope.

[0043] Through the temperature-controlled microscopic system for epidemiological analysis provided above, the temperature can be flexibly and accurately controlled based on the patterned heating layer set in the precise temperature-controlled slide to observe the reaction of microorganisms in the sample liquid at different temperatures, and the sample liquid can be protected by a transparent protective cover to prevent the sample liquid from being contaminated. At the same time, the spread of microorganisms in the sample liquid during the experiment and infection of the staff can be avoided; and, in view of the presence of the transparent protective cover, the microorganisms in the sample liquid can be observed at close range based on an inverted microscope to achieve better observation effects. Furthermore, from an overall analysis, there is no need for repeated temperature treatment and sample preparation, and precise temperature control can be performed during the observation process, making the experimental methods more diverse and the experimental results more accurate. At the same time, the risk of pathogenic bacteria infection caused by repeated sample preparation is avoided.

[0044] In the embodiments of this specification, refer to Figure 4 and Figure 5 As shown, the temperature-controlled microscope system may also include: an electrical connection block 13. The lower surface of the electrical connection block 13 is provided with electrical connection bumps 131 corresponding to the two first contacts ①② and the two second contacts ③④, respectively. Four electrical connection bumps 131 are shown in the figure, and each electrical connection bump 131 can be electrically connected to the internal bus through a wire or a spring, wherein the electrical connection bump 131 can be a conductive column nested in the small hole groove and can be freely extended and retracted; before the electrical connection block 13 is not placed on the precise temperature-controlled slide 12, the electrical connection bump 131 protrudes a part of the surface of the electrical connection block 13, and after the electrical connection block 13 is placed on the precise temperature-controlled slide 12, the electrical connection bump 131 is respectively in contact with the first contact and the second contact, and is compressed back to a part of the small hole groove.

[0045] Meanwhile, a microscope interface 132 for connecting the inverted microscope 11 is provided on the side surface of the electrical connection block 13; if the temperature control unit is integrated inside the inverted microscope 11, the patterned heating layer 123 and the temperature sensing element 122 can be connected to the temperature control unit integrated in the inverted microscope 11 through the microscope interface 132, respectively. Preferably, the microscope interface 132 of the electrical connection block 13 can be a USB interface, and the inverted microscope or the temperature control unit is connected through a USB interface data cable. .

[0046] Optionally, in the embodiment of this specification, the thickness of the patterned heating layer 123 may be in the range of 20-200 nm.

[0047] Accordingly, in order to ensure the surface flatness of the precise temperature-controlled glass slide 12 and simplify the process, the thickness of the temperature sensing element 122 is equal to the thickness of the patterned heating layer 123. It should be understood that Figure 2The thickness of the patterned heating layer 123 and the thickness of the protective layer 124 are not consistent with the actual proportion. For the convenience of display, the proportion relationship is exaggerated. The actual film thickness is much thinner than shown in the figure.

[0048] Optionally, the material of the protective layer 124 is an insulating material, such as silicon oxide, etc., preferably silicon dioxide SiO2 can be used; the thickness of the protective layer 124 can range from 20-1000 nm.

[0049] Optionally, the electrical connection pressing block 13 is filled with a weight material for maintaining balance. Figure 4 As shown, the upper part of the electrical connection pressing block 13 is filled with a weight 133 , and the material of the weight 133 can be metal or other high-density materials.

[0050] The temperature-controlled microscope system for epidemiological analysis mentioned above is described in detail below based on existing usage scenarios, specifically taking the observation of the response of Yersinia pestis to temperature as an example.

[0051] After Yersinia pestis is cultured in a petri dish for 48 hours, a portion of the culture fluid is collected as a sample and dripped onto a precision temperature-controlled slide. Specifically, it should be dripped into the sample detection area covered by a transparent protective cover. Figure 1 As shown, after being covered with a cover glass, the precision temperature-controlled slide 12 is placed on the stage 111 of the inverted microscope 11, and the initial state is observed through the objective lens 112, and finally emitted to the component 113 through the light path composed of multiple optical elements 114, wherein the component 113 can be an eyepiece to facilitate the staff to observe the plague bacteria in the sample liquid, or the component 113 can also be a camera for capturing the reaction image of the plague bacteria in the sample liquid.

[0052] It should be understood that the above-mentioned step of covering the cover glass is an optional step. It is also possible not to cover the cover glass. The sample liquid can be directly dripped onto the precision temperature-controlled slide and then protected by a transparent protective cover to achieve the purpose of safe observation.

[0053] The sample liquid was heated by the patterned heating layer on the precisely controlled glass slide, and the temperature was increased to 37 degrees Celsius, maintained for 12 hours, and the reproduction was observed and images were taken; then the temperature was raised to 70 degrees Celsius and maintained for 1 hour, and then cooled to 37 degrees Celsius, maintained for 12 hours, and the reproduction was observed and images were taken. This can provide scientific research data on the ability of plague bacteria to resist high temperatures.

[0054] After the experiment, the temperature can be raised to 130 degrees Celsius and maintained for a certain period of time (the duration is selected according to the type of microorganisms in the experiment, and 10 minutes is preferred) to completely inactivate viruses, bacteria, etc. in the sample liquid. In this way, the precision temperature-controlled slide can be reused as a medical item, or it can be discarded as a disposable medical item.

[0055] This embodiment provides an operating method of a temperature-controlled microscopy system, comprising the following steps:

[0056] The first step is to drop a sample liquid into the sample detection area on the precise temperature-controlled slide, wherein the sample liquid contains viruses and / or bacteria;

[0057] The second step is to cover the transparent protective cover on the precise temperature-controlled slide, with the lower edge of the transparent protective cover placed in the groove;

[0058] The third step is to drip a sufficient amount of disinfectant into the liquid storage tank so that the groove is filled with the disinfectant;

[0059] Step 4: placing the precise temperature-controlled slide on an inverted microscope for observation, and the user controls the temperature control unit to keep the sample liquid at different temperatures;

[0060] Step 5: 3-12 hours after the execution of step 3, the disinfectant in the reservoir is sucked out and new disinfectant is injected; this step is repeated every 3-12 hours;

[0061] Step 6: Control the temperature control unit to raise the temperature of the sample solution to 130 degrees Celsius and maintain it for 10 minutes.

[0062] Compared with the prior art, when using the temperature-controlled microscope system in the embodiment of this specification to conduct experimental observations of viruses, bacteria and other microorganisms, there is no need to repeatedly perform temperature treatment and sample preparation. Accurate temperature control can be performed during the observation process, the experimental means are flexible and diverse, and the experimental results are more accurate. In addition, the risk of pathogenic bacteria infecting experimental operators due to repeated sample preparation is avoided. At the same time, a transparent protective cover is used to protect the sample liquid to prevent the sample liquid from being contaminated and spreading to infect experimental operators.

[0063] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0064] The above is only an embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A temperature-controlled microscopy system for epidemiological analysis, characterized in that: At least: An inverted microscope and a precision temperature-controlled slide for use with the inverted microscope; wherein: The precise temperature-controlled glass slide comprises: a glass slide base, a temperature sensing element and a patterned heating layer located on a surface of the glass slide base and arranged in a sample detection area, a protective layer at least covering the patterned heating layer and the temperature sensing element, and a transparent protective cover located above the protective layer; the glass slide is made of polymethyl methacrylate PMMA, polystyrene PS or polycarbonate PC; The surface of the glass slide base is also provided with a groove surrounding the sample detection area, the lower opening edge of the transparent protective cover is placed in the groove to at least cover the sample detection area therein, and disinfectant is dripped into the groove during sample detection; The patterned heating layer extends through the groove to extend two first contacts at one end of the surface, outside the sample detection area. At the same time, the temperature sensing element also extends two second contacts at one end of the surface. The two first contacts are used to connect to the output end of the temperature control unit, and the two second contacts are used to connect to the input end of the temperature control unit, so as to control the sample liquid dripped in the sample detection area to reach a preset temperature according to user settings.

2. The temperature-controlled microscopy system for epidemiological analysis according to claim 1, characterized in that: Also includes: Electrical connection block; The lower surface of the electrical connection pressing block is provided with electrical connection bumps corresponding to the first contact and the second contact respectively, and the side surface of the electrical connection pressing block is provided with a microscope interface connected to the inverted microscope; After the electrical connection pressing block is placed correspondingly on the glass slide substrate, the electrical connection bumps are in contact with the first contact and the second contact respectively; the patterned heating layer and the temperature sensing element are respectively connected to the temperature control unit integrated in the inverted microscope through the microscope interface.

3. The temperature-controlled microscopy system for epidemiological analysis according to claim 2, characterized in that: A liquid storage tank is provided on the upper surface of the glass slide base, and a liquid conducting groove connects the liquid storage tank and the groove; a flange is provided on the upper opening of the liquid storage tank and the liquid conducting groove to allow at least a part of the liquid level in the liquid storage tank (127) and / or the liquid conducting groove (129) to be slightly higher than the upper surface of the glass slide base (121).

4. The temperature-controlled microscopy system for epidemiological analysis according to any one of claims 1 to 3, characterized in that: The material of the patterned heating layer is ITO and / or metal.

5. The temperature-controlled microscopy system for epidemiological analysis according to any one of claims 1 to 3, characterized in that: The thickness of the patterned heating layer is in the range of 20-200 nm.

6. The temperature-controlled microscopy system for epidemiological analysis according to claim 5, characterized in that: The thickness of the temperature sensing element is equal to the thickness of the patterned heating layer.

7. The temperature-controlled microscopy system for epidemiological analysis according to any one of claims 1 to 3, characterized in that: The material of the protective layer is insulating material, and the thickness of the protective layer ranges from 20 to 1000 nm.

8. The temperature-controlled microscopy system for epidemiological analysis according to claim 2, characterized in that: The electrical connection pressing block is filled with a weight material for maintaining balance.

9. The temperature-controlled microscopy system for epidemiological analysis according to claim 2 or 8, characterized in that: The microscope interface of the electrical connection pressing block is a USB interface.

10. A method for operating a temperature-controlled microscopy system as claimed in claim 3, comprising the following steps: The first step is to drop a sample liquid into the sample detection area on the precise temperature-controlled slide, wherein the sample liquid contains viruses and / or bacteria; The second step is to cover the transparent protective cover on the precise temperature-controlled slide, with the lower edge of the transparent protective cover placed in the groove; The third step is to drip a sufficient amount of disinfectant into the liquid storage tank so that the groove is filled with the disinfectant; Step 4: placing the precise temperature-controlled slide on an inverted microscope for observation, and the user controls the temperature control unit to keep the sample liquid at different temperatures; Step 5: 3-12 hours after the execution of step 3, the disinfectant in the reservoir is sucked out and new disinfectant is injected; this step is repeated every 3-12 hours; Step 6: Control the temperature control unit to raise the temperature of the sample solution to 130 degrees Celsius and maintain it for 10 minutes.

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