Magnetic field switching rotating parts, magnetic field switching devices, light detection devices, heat preservation devices

Abstract

The present invention relates to the field of detection technology, and specifically to a magnetic field switching rotating part, a magnetic field switching device, a light detection device, and a heat preservation device. The magnetic field switching rotating part proposed by the present invention includes a magnetic member magnetic ring for placing a microfluidic chip, and the magnetic member magnetic ring includes at least one magnetic member and at least one abutment portion. The magnetic field switching rotating part provided by the present invention does not require an external electromagnet coil setting, and meets the needs of a fixed magnet device; the magnetic field switching device can be used to easily manipulate magnetic particles, greatly reducing the complexity of a centrifugal microfluidic system, and can also cooperate with multiple working areas on the microfluidic chip to simultaneously perform cleaning, incubation and mixing steps; the light detection device and the magnetic field switching device are arranged in the same module, which reduces the error of light detection; the heat preservation device including the magnetic field switching device can provide a suitable reaction temperature for the sample in the microfluidic chip.

Description

Technical Field

[0001] The present invention relates to the field of biochemistry, immunity and molecular detection technology, and in particular to a magnetic field switching rotating part, a magnetic field switching device, a light detection device and a heat preservation device. Background Art

[0002] Since magnetic particles have a large specific surface area and paramagnetic properties, they can move quickly (aggregate, disperse) under the action of a magnetic field to improve reaction efficiency and detection sensitivity. They are often used in immune and molecular detection reactions: antibodies / antigens are bound to the surface of magnetic particles, and magnetic particles are manipulated to be cultured, washed, eluted, reacted, and other steps with samples. However, the application of magnetic particles in centrifugal microfluidic systems is hindered by the driving force of the system itself (centrifugal force, Coriolis force), and it is not easy to manipulate magnetic particles in the microfluidic reaction tank to achieve the steps of cultivation, washing, and reaction. The existing method often uses an external (fixed) permanent magnet or (switching) electromagnet to manipulate magnetic particles. The placement of the fixed magnet and the setting of the coil of the switching electromagnet can easily increase the complexity of the centrifugal microfluidic system. The existing magnetic field switching device can often only support one working area of ​​the microfluidic chip, and the detection efficiency is low. In addition, the light detection device of the existing chemiluminescence detector is often set separately from the reaction module of the microfluidic chip. The process is affected by environmental factors and operations, which often leads to large errors in the detection results. Summary of the invention

[0003] In order to overcome the shortcomings of the prior art, the present invention proposes a magnetic field switching rotating part, a magnetic field switching device, a light detection device, and a heat preservation device, and adopts a magnetic field switching rotating part to replace a complex fixed magnet; the magnetic field switching device proposed in the present invention utilizes a simple magnetic field switching device to manipulate magnetic particles, and simultaneously completes the cleaning and incubation mixing steps of multiple working areas; the light detection device proposed in the present invention utilizes a light detection device arranged on one side of the magnetic field switching rotating part to realize rapid detection of samples in a microfluidic chip; the heat preservation device proposed in the present invention can provide a suitable reaction temperature according to the actual needs of the sample reaction.

[0004] The present invention provides a magnetic field switching rotating member, comprising a magnetic member magnetic ring for placing a microfluidic chip, wherein the magnetic member magnetic ring comprises at least one magnetic member and at least one abutment; wherein the magnetic member is used to manipulate magnetic particles in a magnetic adsorption zone of the microfluidic chip; the abutment limits the movement of the magnetic field switching rotating member under the action of an external force, so that the magnetic field switching rotating member can move relative to the magnetic adsorption zone of the microfluidic chip, so as to switch the position of the magnetic member relative to the magnetic adsorption zone of the microfluidic chip, thereby changing the magnetic force of the magnetic member on the magnetic particles in the magnetic adsorption zone.

[0005] Preferably, the interference portion is a boss.

[0006] Preferably, the interference portion is a recessed portion or a cylinder.

[0007] Preferably, the interference portion is distributed on the outer circumference of the magnetic ring of the magnetic member.

[0008] Preferably, the plurality of magnetic members are arranged on a circle with the same radius on the magnetic ring of the magnetic member.

[0009] Preferably, the plurality of magnetic members are arranged on circles with different radii on the magnetic ring of the magnetic member.

[0010] Preferably, the magnetic ring of the magnetic member is made of transparent material.

[0011] Preferably, the magnetic ring of the magnetic member includes at least one luminous detection area.

[0012] Preferably, the luminescence detection area is a through hole.

[0013] Preferably, the luminescent detection area is made of glass, plastic or film.

[0014] Preferably, the luminescent detection area and the magnetic member are arranged in pairs, and each pair of the luminescent detection area and the magnetic member corresponds to a working area of ​​the microfluidic chip.

[0015] The present invention also relates to a magnetic field switching device, including the magnetic field switching rotating member, a disk support and a magnetic member lever module. The magnetic field switching rotating member rotates in the disk support, and the magnetic member lever module contacts the resistance part of the magnetic ring of the magnetic member to hinder the rotation of the magnetic field switching rotating member to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip.

[0016] Preferably, the disk holder comprises a side wall, and a limiting area is arranged on the side wall, and the limiting area limits the position range of the magnetic member moving relative to the magnetic adsorption area of ​​the microfluidic chip.

[0017] Preferably, the number of the magnetic members is n, and the limiting area limits the position range of the magnetic members relative to the magnetic adsorption area of ​​the microfluidic chip to be between -(360 / 2n)° and +(360 / 2n)°.

[0018] Preferably, the limiting area is provided with a detection device to detect the position of the interference portion in the limiting area.

[0019] Preferably, the magnetic member lever module comprises: a lever, an actuator, a magnetic member lever module fixing frame, and a limit switch; the magnetic member lever module fixing frame is used to fix the magnetic member lever module, and the actuator triggers the limit switch to generate a signal, and the signal triggers to drive the lever to contact and hinder the rotation of the magnetic field switching rotating member to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip.

[0020] The present invention also relates to a light detection device, comprising a magnetic field switching device and a light detection probe, wherein the light detection device is arranged on one side of the magnetic field switching device to detect the luminous intensity of a light beam passing through a luminous detection area, and the light detection probe is connected to a photomultiplier tube.

[0021] Preferably, when the magnetic member lever module does not contact the magnetic member magnetic ring resistance part, the light detection probe is located directly below or above the luminous detection area; when the magnetic member lever module contacts the resistance part and hinders the rotation of the magnetic field switching rotating member, the light detection probe is located below or above the luminous detection area.

[0022] Preferably, the magnetic ring of the magnetic component is made of transparent material, and when the magnetic component lever module does not contact the resistance part of the magnetic ring of the magnetic component, the light detection probe is located below or above the deviated magnetic component; when the magnetic component lever module contacts the resistance part and hinders the rotation of the magnetic field switching rotating component, the light detection probe is located directly below or directly above the magnetic component.

[0023] The present invention also relates to a heat preservation device, comprising a warming body for warming the microfluidic chip, wherein the warming body comprises the magnetic field switching device.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention provides a magnetic field switching rotating member, a magnetic field switching device, a light detection device and a heat preservation device. The magnetic field switching rotating member includes a magnetic member magnetic ring for placing a microfluidic chip. The magnetic member magnetic ring is provided with magnetic members and a resistance part. The resistance part limits the rotation of the magnetic field switching rotating member under the action of an external force. The magnetic field switching rotating member proposed by the present invention does not require an external electromagnet coil setting, and can greatly reduce the complexity of the centrifugal microfluidic system.

[0026] The magnetic field switching device provided by the present invention includes a magnetic field switching return transmission member, a disk support seat and a magnetic member lever module. The magnetic field switching rotating member rotates in the disk support seat, and the magnetic member lever module contacts the magnetic ring contact portion of the magnetic member and hinders the movement of the magnetic field switching rotating member, so that the magnetic field switching rotating member can move relative to the magnetic adsorption area of ​​the microfluidic chip to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip. The magnetic field switching device proposed by the present invention can easily manipulate magnetic particles, and can also cooperate with multiple working areas on the microfluidic chip to perform cleaning and incubation mixing operations at the same time.

[0027] The light detection device provided by the present invention comprises a magnetic field switching device and a light detection probe, wherein the light detection device is arranged on one side of the magnetic field switching device to detect the luminous intensity of a light beam passing through a luminous detection area, and the light detection probe is connected to a photomultiplier tube. The light detection device and the magnetic field switching device provided by the present invention are arranged in the same module, thereby reducing the error of light detection.

[0028] The heat preservation device provided by the present invention comprises a heating body for heating a microfluidic chip and a magnetic field switching device. The heat preservation device provided by the present invention can provide a suitable reaction temperature according to the particularity of the sample.

[0029] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. The specific implementation of the present invention is given in detail by the following embodiments and their accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0031] Figure 1 It is a schematic diagram of the structure of the present invention;

[0032] Figure 2 It is a structural diagram of the microfluidic chip holder of the present invention;

[0033] Figure 3 It is a schematic diagram of the structure of the magnetic member lever of the present invention;

[0034] Figure 4 is an illustration of the disk-type microfluidic chip of the present invention;

[0035] Figure 5 It is a schematic diagram of a magnetic field switching rotating member of the present invention;

[0036] Figure 6 It is a structural schematic diagram of a disk support and a magnetic field switching rotating member in the magnetic switching device of the present invention;

[0037] Figure 7 It is another structural schematic diagram of the disk support and the magnetic field switching rotating member in the magnetic switching device of the present invention;

[0038] Figure 8 It is a schematic diagram of the optical detection device and the magnetic ring of the magnetic member in the optical detection device of the present invention;

[0039] Fig. 9 It is a schematic structural diagram of the heat preservation device of the present invention.

[0040] As shown in the figure: 101, rotating module: 11, servo motor fixing seat, 12, disc support fixing frame, 13, disc support, 14, magnetic ring of magnetic part, 15, pressure plate, 16, servo motor, 17, magnetic part, 18, luminous detection area, 19, resistance part, 20, side wall, 21, limiting area, 22, one side of the side wall, 23, the other side of the side wall;

[0041] 102. Microfluidic chip holder module;

[0042] 103. Magnetic lever module: 31. Cylinder, 32. Dual-axis electromagnetic actuator, 33. Limit switch signal, 34. Magnetic lever module fixing bracket;

[0043] 104. Disc microfluidic chip: 41. positioning hole, 42. sample slot, 43. first microfluidic valve, 44. magnetic microsphere storage slot, 45. second microfluidic valve, 46. first reagent storage slot, 47. third microfluidic valve, 48. second reagent storage slot, 49. fourth microfluidic valve, 50. third reagent storage slot, 51. fifth microfluidic valve, 52. fourth reagent storage slot, 53. sixth microfluidic valve, 54. fifth reagent storage slot, 55. seventh microfluidic valve, 56. sixth reagent storage slot;

[0044] 105. Light detection device: 61. Light detection probe, 62. Photomultiplier tube;

[0045] 106. Heat preservation device. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0047] like Figure 1-Figure 4 As shown, Figure 1 It is a structural schematic diagram of the present invention, Figure 2 is a structural diagram of the microfluidic chip holder of the present invention, Figure 3 is a schematic diagram of the magnetic lever structure of the present invention, Figure 4 It is an explanatory diagram of the disk-type microfluidic chip of the present invention.

[0048] Next, the present invention will be further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0049] Embodiment 1

[0050] like Figure 1-2 As shown, a magnetic field switching device described in the present invention includes a rotating module 101 that can provide driving force to drive magnetic particles to move in a microfluidic reaction tank and a microfluidic chip 104 placed in a microfluidic chip support module 102. The rotating module 101 provides control power for the magnetic member lever module 103.

[0051] The rotating module 101 includes: a servo motor fixing seat 11, a disk support fixing frame 12, a disk support 13, a magnetic ring 14, a pressure plate 15, a servo motor 16, and a magnetic member 17; the servo motor 16 is fixed to the rotating module 101 by the servo motor fixing seat 11, the disk support 13 above the microfluidic chip support module 102 is covered with a magnetic ring 14, and a pressure plate 15 is covered above the magnetic ring 14, the disk support 13 is installed in the disk support fixing frame 12 and fixed above the servo motor 16, and the magnetic member 17 is a permanent magnet.

[0052] like Figure 3 As shown, the magnetic lever module 103 includes: a cylinder 31, a dual-axis electromagnetic actuator 32, a limit switch signal 33, and a magnetic lever module fixing frame 34; the microfluidic chip support module 102 and the magnetic lever module 103 control the movement of magnetic particles in the centrifugal microfluidic reaction tank to achieve the steps of cultivation, cleaning, elution, reaction, etc.

[0053] like Figure 4 As shown, the disc-type microfluidic chip 104 comprises: a positioning hole 41, a sample slot 42, a first microfluidic valve 43, a magnetic microsphere storage slot 44 (magnetic particles are bound to antibodies / antigens on their surfaces, i.e., magnetic microspheres, which are pre-packaged in the magnetic microsphere storage slot 44), a second microfluidic valve 45, a first reagent storage slot 46 (a first reagent is pre-packaged in the first reagent storage slot), a third microfluidic valve 47, a second reagent storage slot 48 (a second reagent is pre-packaged in the second reagent storage slot), a fourth microfluidic valve 49, a third reagent storage slot 50 (a third reagent is pre-packaged in the third reagent storage slot), a fifth microfluidic valve 51, a fourth reagent storage slot 52 (a fourth reagent is pre-packaged in the fourth reagent storage slot), a sixth microfluidic valve 53, a fifth reagent storage slot 54 (a fifth reagent is pre-packaged in the fifth reagent storage slot), a seventh microfluidic valve 55, and a sixth reagent storage slot 56 (a sixth reagent is pre-packaged in the sixth reagent storage slot). The disc-type microfluidic chip 104 is placed in the microfluidic chip holder module 102.

[0054] Embodiment 2

[0055] Procalcitonin PCT test

[0056] like Figure 4 As shown, 20 microliters of serum is injected into the sample tank 42, and the disk chip 104 is rotated at 3,000 RPM (acceleration 5,000 RPM / s). The sample is driven by centrifugal force to break through the first microfluidic valve 43 and is transferred to the magnetic microsphere storage tank 44. The disk chip is rotated clockwise and counterclockwise at 5 Hz and 135 degrees to mix the sample and the magnetic microspheres to achieve the incubation / reaction effect.

[0057] Furthermore, the magnetic ring 14 is controlled to rotate 135°, so that the reacted magnetic microspheres break through the second microfluidic valve 45 from the magnetic microsphere storage tank 44 and are transferred to the first reagent storage tank 46. When the cylinder returns to the original position, the disk chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 55° to mix the reacted magnetic microspheres with 15 microliters of enzyme label 1 to achieve the incubation / reaction effect.

[0058] Furthermore, the magnetic ring 14 is controlled to rotate 45°, so that the reacted magnetic microspheres break through the third microfluidic valve 47 from the first reagent storage tank 46 and are transferred to the second reagent storage tank 48. When the cylinder 31 returns to the original position, the disk chip is controlled to rotate clockwise and counterclockwise at 3 Hz and 105° to mix the reacted magnetic microspheres and 15 microliters of enzyme label 2 to achieve the incubation / reaction effect.

[0059] Furthermore, the magnetic ring 14 is controlled to rotate 45°, so that the reacted magnetic microspheres break through the fourth microfluidic valve 49 from the second reagent storage tank 48 and are transferred to the third reagent storage tank 50. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 35° to mix the reacted magnetic microspheres with 30 microliters of cleaning solution to achieve the effect of the first cleaning.

[0060] Furthermore, the magnetic ring 14 is controlled to rotate 45°, so that the reacted magnetic microspheres break through the fifth microfluidic valve 51 from the third reagent storage tank 50 and are transferred to the fourth reagent storage tank 52. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 35° to mix the reacted magnetic microspheres with 30 microliters of cleaning solution to achieve the effect of the second cleaning.

[0061] Furthermore, the magnetic ring 14 is controlled to rotate 45°, so that the reacted magnetic microspheres break through the sixth microfluidic valve 53 from the fourth reagent storage tank 52 and are transferred to the fifth reagent storage tank 54. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 35° to mix the reacted magnetic microspheres with 30 microliters of cleaning solution to achieve the effect of the third cleaning.

[0062] Furthermore, the magnetic ring 14 is controlled to rotate 45°, so that the reacted magnetic microspheres break through the seventh microfluidic valve 55 from the fifth reagent storage tank 54 and are transferred to the sixth reagent storage tank 56. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 6Hz and 90° to mix the reacted magnetic microspheres and 30 microliters of substrate to achieve the effect of incubation / reaction. After the reaction is completed for 300 seconds, chemiluminescence detection can be performed.

[0063] Embodiment 3

[0064] Extraction and purification of free DNA in peripheral blood circulation

[0065] like Figure 4 As shown, 100 microliters of serum is injected into the sample tank, and the disc chip is rotated at 2,500 RPM (acceleration 4,000 RPM / s). The sample is driven by centrifugal force to break through the first microfluidic valve 43 and is transferred to the magnetic microsphere storage tank 44. The disc chip is rotated clockwise and counterclockwise at 10 Hz and 75 degrees to mix the sample and the magnetic microspheres to achieve the effect of incubation / reaction.

[0066] Furthermore, the magnetic ring 14 is controlled to rotate 120°, so that the reacted magnetic microspheres break through the second microfluidic valve 45 from the magnetic microsphere storage tank 44 and are transferred to the first reagent storage tank 46. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 5 Hz and 120° to mix the reacted magnetic microspheres with 80 microliters of lysis solution to achieve the first lysis effect.

[0067] Furthermore, the magnetic ring 14 is controlled to rotate 30°, so that the reacted magnetic microspheres break through the third microfluidic valve 47 from the first reagent storage tank 46 and are transferred to the second reagent storage tank 48. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 5 Hz and 80° to mix the reacted magnetic microspheres with 60 microliters of lysis solution to achieve the effect of the second lysis.

[0068] Furthermore, the magnetic ring 14 is controlled to rotate 30°, so that the reacted magnetic microspheres break through the fourth microfluidic valve 49 from the second reagent storage tank 48 and are transferred to the third reagent storage tank 50. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 35° to mix the reacted magnetic microspheres with 100 microliters of alcohol to achieve the effect of the first cleaning.

[0069] Furthermore, the magnetic ring 14 is controlled to rotate 30°, so that the reacted magnetic microspheres break through the fifth microfluidic valve 51 from the third reagent storage tank 50 and are transferred to the fourth reagent storage tank 52. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 8 Hz and 35° to mix the reacted magnetic microspheres with 80 microliters of alcohol to achieve the effect of the second cleaning.

[0070] Furthermore, the magnetic ring 14 is controlled to rotate 30°, so that the reacted magnetic microspheres break through the sixth microfluidic valve 53 from the fourth reagent storage tank 52 and are transferred to the fifth reagent storage tank 54. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 6 Hz and 30° to mix the reacted magnetic microspheres with 60 microliters of alcohol to achieve the third cleaning effect.

[0071] Furthermore, the magnetic ring 14 is controlled to rotate 30°, so that the reacted magnetic microspheres break through the seventh microfluidic valve 55 from the fifth reagent storage tank 54 and are transferred to the sixth reagent storage tank 56. When the cylinder 31 returns to the original position, the disc chip is controlled to rotate clockwise and counterclockwise at 10 Hz and 30° to mix the reacted magnetic microspheres with 60 microliters of deionized water to achieve the effect of eluting nucleic acid molecules, and the purification reaction is completed.

[0072] like Figure 5-Figure 9 As shown, Figure 5 It is a schematic diagram of the magnetic field switching rotating member of the present invention. Figure 6 It is a structural schematic diagram of a disk support and a magnetic field switching rotating member in the magnetic switching device of the present invention. Figure 7 It is another structural schematic diagram of the disk support and the magnetic field switching rotating member in the magnetic switching device of the present invention. Figure 8 is a schematic diagram of the optical detection device and the magnetic ring of the magnetic member in the optical detection device of the present invention, Fig. 9 It is a schematic structural diagram of the heat preservation device of the present invention.

[0073] Next, the present invention will be further described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0074] Embodiment 4

[0075] A magnetic field switching rotating member, such as Figure 1 As shown, it includes a magnetic ring 14 for placing a microfluidic chip, and the magnetic ring 14 includes at least one magnetic member 17 and at least one abutment 19. When the magnetic field switching rotating member rotates, the position of the magnetic member is driven to change. The magnetic member is preferably a permanent magnet, and the abutment 19 limits the movement of the magnetic field switching rotating member under the action of an external force, that is, the driving magnetic field switching rotating member can move relative to the magnetic adsorption area of ​​the microfluidic chip to switch the position of the magnetic member 17 relative to the magnetic adsorption area of ​​the microfluidic chip, thereby changing the magnetic force of the magnetic member 17 on the magnetic particles in the magnetic adsorption area. The magnetic adsorption area of ​​a microfluidic chip refers to an area that has been or is about to be loaded with magnetic particles.

[0076] In order to facilitate the manipulation of the magnetic ring 14, preferably, two abutment portions 19 are provided on the magnetic ring, and the two abutment portions 19 are distributed on the outer circumference of the magnetic ring 14. When an external force contacts the abutment portion 19, the movement of the magnetic ring 14 is restricted. The abutment portion 19 can be a component of any shape, preferably a boss, a depression or a cylinder.

[0077] The magnetic member 17 is arranged on the circumference of the same radius on the magnetic member magnetic ring 14, and the magnetic adsorption area of ​​the microfluidic chip is also arranged on the circumference of the same radius, and the center of the magnetic member magnetic ring and the center of the microfluidic chip are on the same vertical line (perpendicular to the horizontal plane). When the number of magnetic members is different from the number of magnetic adsorption areas of the microfluidic chip, such as there is only one magnetic member 17 and there are multiple magnetic adsorption areas of the microfluidic chip, the abutment 19 limits the movement of the magnetic member magnetic ring 14 under the action of external force, that is, the magnetic member magnetic ring 14 moves relative to the magnetic adsorption area of ​​the microfluidic chip 104, and can drive the magnetic particles to move at any angle in the microfluidic chip, such as 360° movement, to complete the steps of mixing, cultivating, and cleaning the magnetic particles with samples and reagents.

[0078] When the number of magnetic parts is the same as the number of magnetic adsorption areas of the microfluidic chip, for example, the number of magnetic parts 17 on the magnetic ring of the magnetic part is 8, which are distributed on the circumference of the magnetic ring 14 of the magnetic part with the same radius, for example, the radius is R1. The microfluidic chip can be circular, and 8 magnetic adsorption areas are arranged on it, which are distributed on the circumference of the microfluidic chip 104 with the same radius, for example, the radius is R2. The magnetic parts 17 correspond to the magnetic adsorption areas on the microfluidic chip one by one, so that different magnetic parts 17 can independently control a magnetic adsorption area. Here, the one-to-one correspondence means that the magnetic parts and the magnetic adsorption areas of the microfluidic chip are at the same radius of different circles on the same vertical line (perpendicular to the horizontal plane). That is, the center of the magnetic ring 14 of the magnetic part and the center of the microfluidic chip are on the same vertical line (perpendicular to the horizontal plane) and R1=R2. The resistance part 19 limits the movement of the magnetic member magnetic ring 14 under the action of external force, that is, drives the magnetic member magnetic ring 14 to move relative to the magnetic adsorption area of ​​the microfluidic chip 104, switches the position of the magnetic member 17 relative to the magnetic adsorption area of ​​the microfluidic chip 104, and manipulates the 8 magnetic members 17 to respectively adsorb the magnetic particles in the magnetic adsorption areas of the 8 microfluidic chips to fix the magnetic particles, thereby facilitating the cleaning of the magnetic particles.

[0079] In one embodiment, the magnetic member 17 can be arranged on the circumference of different radii on the magnetic member magnetic ring 14. The magnetic member magnetic ring 14 is provided with a plurality of magnetic members 17 on the circumference of different radii. When the number of magnetic members 17 is different from the number of magnetic adsorption areas on the microfluidic chip 104, such as there is only one magnetic member 17 on the circumferences of different radii, and there are multiple magnetic adsorption areas on the circumferences of different radii of the microfluidic chip, the abutment 19 limits the movement of the magnetic member magnetic ring 14 under the action of external force, that is, the magnetic member magnetic ring 14 moves relative to the magnetic adsorption area of ​​the microfluidic chip 104, and drives the magnetic particles on the circumferences of different radii to move at any angle in the microfluidic chip, such as 360° movement, so as to facilitate the completion of the steps of mixing, cultivating, and cleaning of multiple magnetic particles with samples and reagents.

[0080] When the number of magnetic parts is the same as the number of magnetic adsorption areas of the microfluidic chip, Figure 5 As shown, 4 magnetic members are provided on the magnetic ring of the magnetic member at the radius R3, and 8 magnetic members 17 are provided at the radius R4, wherein R3 is not equal to R4. The microfluidic chip 104 is provided with 4 magnetic adsorption areas on the circumference of the radius R5, and 8 magnetic adsorption areas on the circumference of the radius R6, wherein R5 is not equal to R6. The magnetic members 17 correspond one-to-one with the magnetic adsorption areas on the microfluidic chip 104, so that different magnetic members 17 can independently control a magnetic adsorption area, that is, the center of the magnetic ring 14 of the magnetic member and the center of the microfluidic chip 104 are on the same vertical line (perpendicular to the horizontal plane) and R3=R5, R4=R6. The resistance part 19 limits the movement of the magnetic member magnetic ring 14 under the action of external force, and the magnetic members 17 at different positions on the magnetic member magnetic ring 14 move relative to the magnetic adsorption areas at different positions of the microfluidic chip 104, switching the position of the magnetic member 17 relative to the magnetic adsorption area of ​​the microfluidic chip 104, and manipulating the magnetic members 17 at different positions to respectively adsorb the magnetic particles in the corresponding magnetic adsorption areas of the microfluidic chip 104 to fix the magnetic particles, thereby facilitating the cleaning of the magnetic particles.

[0081] The magnetic member 17 on the magnetic ring 14 can move at any angle relative to the magnetic adsorption area on the microfluidic chip 104. In one embodiment, the magnetic member 17 on the magnetic ring 14 moves 360° relative to the magnetic adsorption area at different positions of the microfluidic chip 104. Figure 4As shown, when the magnetic member 17 adsorbs the magnetic particles in the magnetic microsphere storage tank 44 (i.e., the magnetic adsorption area of ​​the microfluidic chip), under the action of the driving force, the magnetic member magnetic ring 14 is manipulated to move relative to the magnetic adsorption area of ​​the microfluidic chip, so that the magnetic member 17 moves from below the magnetic microsphere storage tank 44 to below the first reagent storage tank 46, and the reacted magnetic particles break through the second microfluidic valve 45 from the magnetic microsphere storage tank 44 and are transferred to the first reagent storage tank 46 (because the first reagent storage tank is loaded with magnetic particles at this time, it can be used as the magnetic adsorption area of ​​the microfluidic chip). When the magnetic member magnetic ring 14 is continued to be manipulated to move relative to the magnetic adsorption area of ​​the microfluidic chip, the magnetic member 17 will drive the magnetic particles to continue to move, and the magnetic particles are sequentially mixed with the reagents in the second reagent storage tank 48, the third reagent storage tank 50, the fourth reagent storage tank 52, the fifth reagent storage tank 54, and the sixth reagent storage tank 56, completing the cleaning and incubation mixing steps of the magnetic particles.

[0082] In another embodiment (not shown), the range of motion of the magnetic member 17 on the magnetic ring 14 relative to the magnetic adsorption area on the microfluidic chip 104 is related to the number of magnetic members. When the number of magnetic members is n, and the number of magnetic adsorption areas of the microfluidic chip is n, the range of motion is between (-360 / 2n)° and (360 / 2n)°. That is, the magnetic particles only move in a limited angle in a magnetic adsorption area of ​​the microfluidic chip, which facilitates the cleaning of the magnetic particles.

[0083] The magnetic ring 14 may be a magnetic ring made of a transparent material, on which at least one magnetic member 17 is distributed. In one embodiment (not shown), the magnetic ring 14 may be made of an opaque material, on which a luminescent detection area 18 is provided. The luminescent detection area 18 is preferably a through hole, which is convenient for light to pass directly through, and the through hole may be of any shape, such as: circular or square. In other embodiments, the luminescent detection area 18 may be an area formed by a material with good light transmittance, and preferably, the material with good light transmittance is glass, plastic or film. The luminescent detection area 18 is arranged in pairs with the magnetic member 17, and each pair of luminescent detection areas 18 and magnetic members 17 corresponds to a working area of ​​the microfluidic chip, and the working area of ​​the microfluidic chip refers to the area of ​​the microfluidic chip that can complete the steps of cultivation, cleaning, and reaction.

[0084] Embodiment 5

[0085] like Figure 6 As shown, a magnetic field switching device includes a magnetic field switching rotor and a disc holder 13. The magnetic field switching rotor is arranged in the disc holder 13. The magnetic field switching rotor can move at any angle in the disc holder 13 under the action of a driving force, such as 360° movement.

[0086] like Figure 7As shown, the disk support 13 has a side wall 20, and a limiting area 21 is provided on the side wall 20, for example, the limiting area 21 can be a notch on the side wall 20. Due to the existence of the magnetic ring abutment 19 of the magnetic member, the limiting area 21 limits the motion range of the magnetic field switching rotating body, for example, the motion range of the magnetic ring 14 of the magnetic member is the range corresponding to a notch on the side wall 20 of the disk support. The range corresponding to the notch is related to the number of working areas on the microfluidic chip. The number of working areas of the microfluidic chip is n, and the range corresponding to the notch is between (-360 / 2n)° and (360 / 2n)°. When the number of working areas of the microfluidic chip is 8, the range corresponding to the notch is between -22.5° and +22.5°.

[0087] like Figure 1 As shown, the magnetic field switching device further includes a magnetic member lever module 103, and the magnetic member lever module 103 is arranged to match the position of the resistance part 19, such as the resistance part 19 is arranged at the bottom of the magnetic member magnetic ring 14, and the magnetic member lever module 103 is arranged at the bottom of the magnetic member magnetic ring 14. In one embodiment, the resistance part 19 is arranged on the circumference of the outer ring of the magnetic member magnetic ring 14, and the magnetic member lever module 103 is arranged relative to the resistance part 19 to limit the resistance part when receiving instructions.

[0088] like Figure 7 As shown, the disk holder 13 includes a side wall 20, and a limiting area 21 is provided on the side wall 20, and a detection device is provided in the limiting area 21. For example, the limiting area 21 can be a gap on the side wall 20. Detection devices are provided on both sides of the gap, and the detection devices can be sensors. When the magnetic member lever module 103 does not contact the interference portion 19, the interference portion 19 is located on one side 22 of the side wall, and the sensor at the side 22 of the side wall detects and stores the position of the interference portion 19, and allows the sample and magnetic particles in the microfluidic chip 104 to react, cultivate, and mix. When the magnetic member lever module 103 contacts the interference portion 19 and hinders the rotation of the magnetic field switching rotating body, the magnetic field switching rotating body moves relative to the disk holder 13, and the interference portion 19 of the magnetic member magnetic ring 14 moves from one side 22 of the side wall to the other side 23 of the side wall. The sensor at the other side 23 of the side wall detects and stores the position of the interference portion, and allows the microfluidic chip 104 to perform a cleaning operation. This embodiment can be used to adjust the initial position of the magnetic field switching rotor after placing the microfluidic chip 104 on the disk holder 13 to ensure the normal operation of the magnetic field switching device.

[0089] The magnetic lever module includes: a lever, an actuator, a limit switch (the limit switch generates a signal after the actuator is triggered), and a magnetic lever module fixing frame; the lever can be any element that can limit the interference part, and the shape can match the shape of the interference part. In one embodiment, when the interference part is a boss, the lever is a cylinder. The actuator is used to provide a driving force. In addition to being a dual-axis electromagnetic actuator, the driving force can also be provided by a hydraulic brake, a transmission device, etc.

[0090] like Figure 1 and Figure 3 As shown, the magnetic member lever module fixing frame 34 is used to fix the magnetic member lever module 103, and the dual-axis electromagnetic actuator 32 triggers the limit switch to generate a signal 33, which is triggered to drive the cylinder 31 to contact the abutment portion 19 of the magnetic ring 14 of the magnetic member to limit the rotation of the magnetic field switching rotating body. The microfluidic chip disposed in the disk holder 13 can rotate relative to the magnetic field switching rotating body to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip, thereby changing the magnetic force of the magnetic member on the magnetic particles in the magnetic adsorption area.

[0091] Embodiment 6

[0092] The light detection device is used to detect the luminous intensity of the light beam passing through the luminous detection area, and can be independently set in two separate modules with the magnetic switching device, or can be set in the same module. The light beam passing through the luminous detection area can be absorbed light, fluorescence, chemiluminescence, etc. The light detection device includes a magnetic field switching device and a light detection probe. The light detection device is set on one side of the magnetic field switching device to detect the luminous intensity of the light beam passing through the luminous detection area, and the light detection probe is connected to the photomultiplier tube.

[0093] In one embodiment (not shown), the magnetic ring 14 is made of a transparent material, and a magnetic member 17 is arranged on the magnetic ring. When the magnetic lever module 103 does not contact the abutment portion 19, the light detection probe 61 is located below the transparent portion of the magnetic ring 14, that is, any area except directly below the magnetic member, and the luminous intensity of the light beam passing through the luminous detection area can be detected. When the magnetic lever module 103 contacts the abutment portion 19 and prevents the magnetic field switching rotor from rotating, the light detection probe 61 is located directly below the magnetic member 17 and cannot detect the luminous intensity of the light beam passing through the luminous detection area.

[0094] In one embodiment, if Figure 1 and Figure 8As shown, the luminous detection area 18 on the magnetic ring 14 of the magnetic member is a through hole. When the magnetic member lever module 103 does not contact the abutment portion 19, the light detection probe 61 is located directly below the through hole of the magnetic member magnetic ring 14, and the luminous intensity of the light beam passing through the luminous detection area 18 can be detected. When the magnetic member lever module 103 contacts the abutment portion 19 and hinders the rotation of the magnetic field switching rotating body, the light detection probe 61 is located below the deviation from the through hole, and the luminous intensity of the light beam passing through the luminous detection area cannot be detected. The experimenter can judge whether the magnetic field switching of the magnetic field switching device is completed by the relative position of the luminous detection area and the light detection probe. When the luminous detection area and the light detection probe are in the same vertical position, the magnetic field switching is not completed, and the sample and magnetic particles in the microfluidic chip are in the process of reaction, cultivation, and mixing. When the luminous detection area and the light detection probe are in different vertical positions, the magnetic field switching is completed, and the microfluidic chip is undergoing a cleaning operation.

[0095] The light detection probe 61 is connected to a signal conversion device. The signal conversion device is a photomultiplier tube 62. When the light detection probe receives an instruction, the light detection probe detects the luminous intensity of the light beam passing through the luminous detection area, and the photomultiplier tube converts the light signal into an electrical signal and transmits the data to the controller.

[0096] Embodiment 7

[0097] like Fig. 9 As shown, a heat preservation device 106 includes a heat preservation body for heating the microfluidic chip, the heat preservation body includes a magnetic field switching device, and the heat preservation device provides a reaction environment of a certain temperature for the working area of ​​the microfluidic chip.

[0098] In one embodiment, the heat preservation device 106 is a closed container, including an incubation body for heating the microfluidic chip. The heating of the microfluidic chip is provided by a heating module, and the heating module can be set at any position of the magnetic field switching device, such as above or below the magnetic field switching device. In one embodiment, the heating module is arranged below the magnetic field switching device, and the shape of the heating module matches the shape of the microfluidic chip to supply uniform heat to the microfluidic chip. For example, the microfluidic chip is disc-type, and the heating module is circular or annular. In one embodiment, the heating module can be a circular heating plate. The sample is moved into the microfluidic chip of the heat preservation device. The heat preservation device controls the heating of the heating plate and transfers the heat to the microfluidic chip according to the particularity of the sample reaction, so as to provide the required reaction temperature for different reagents.

[0099] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A magnetic field switching rotating member, comprising a magnetic ring for placing a microfluidic chip, characterized in that: The magnetic ring of the magnetic member includes at least one magnetic member and at least one abutment, and several of the magnetic members are arranged on the circumference of the same radius on the magnetic ring of the magnetic member or several of the magnetic members are arranged on the circumference of the magnetic ring of the magnetic member with different radii; the abutment is distributed on the outer circumference of the magnetic ring of the magnetic member; wherein, The magnetic parts are used to manipulate the magnetic particles in the magnetic adsorption area of ​​the microfluidic chip; The resistance part limits the movement of the magnetic field switching rotating member under the action of external force, so that the magnetic field switching rotating member rotates relative to the magnetic adsorption area of ​​the microfluidic chip to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip, thereby changing the magnetic force of the magnetic member on the magnetic particles in the magnetic adsorption area.

2. The magnetic field switching rotating member according to claim 1, characterized in that: The abutment portion is a boss.

3. The magnetic field switching rotating member according to claim 1, characterized in that: The interference portion is a recessed portion or a cylinder.

4. The magnetic field switching rotating member according to claim 1, characterized in that: The magnetic ring of the magnetic member is made of transparent material.

5. The magnetic field switching rotating member according to claim 1, characterized in that: The magnetic ring of the magnetic member includes at least one luminous detection area.

6. The magnetic field switching rotating member according to claim 5, characterized in that: The luminescence detection area is a through hole.

7. The magnetic field switching rotating member according to claim 5, characterized in that: The luminescent detection area is made of glass, plastic or film.

8. The magnetic field switching rotating member according to claim 5, characterized in that: The luminescent detection area and the magnetic member are arranged in pairs, and each pair of the luminescent detection area and the magnetic member corresponds to a working area of ​​the microfluidic chip.

9. A magnetic field switching device, characterized in that: It includes the magnetic field switching rotating member, the disk support and the magnetic member lever module as described in claim 1, the magnetic field switching rotating member rotates in the disk support, the magnetic member lever module contacts the resistance part of the magnetic ring of the magnetic member, hinders the rotation of the magnetic field switching rotating member, so as to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip.

10. The magnetic field switching device according to claim 9, characterized in that: The disk support seat comprises a side wall, and a limiting area is arranged on the side wall, and the limiting area limits the position range of the magnetic member moving relative to the magnetic adsorption area of ​​the microfluidic chip.

11. The magnetic field switching device according to claim 10, characterized in that: The number of the magnetic members is n, and the limiting area limits the position range of the magnetic members relative to the magnetic adsorption area of ​​the microfluidic chip to be between -(360 / 2n)° and +(360 / 2n)°.

12. The magnetic field switching device according to claim 10, characterized in that: The limiting area is provided with a detection device to detect the position of the interference part in the limiting area.

13. The magnetic field switching device according to claim 9, characterized in that: The magnetic lever module includes: a lever, an actuator, a magnetic lever module fixing frame, and a limit switch; the magnetic lever module fixing frame is used to fix the magnetic lever module, and the actuator triggers the limit switch to generate a signal, which is triggered to drive the lever to contact and hinder the rotation of the magnetic field switching rotating member to switch the position of the magnetic member relative to the magnetic adsorption area of ​​the microfluidic chip.

14. A light detection device, characterized in that: It comprises a magnetic field switching device and a light detection probe as described in claim 12, wherein the light detection device is arranged on a side of the magnetic field switching device to detect the luminous intensity of a light beam passing through a luminous detection area, and the light detection probe is connected to a photomultiplier tube.

15. The light detection device according to claim 14, characterized in that When the magnetic member lever module does not contact the magnetic member magnetic ring resistance part, the light detection probe is located directly below or above the luminous detection area; when the magnetic member lever module contacts the resistance part and hinders the rotation of the magnetic field switching rotating member, the light detection probe is located below or above the luminous detection area.

16. The light detection device according to claim 14, characterized in that The magnetic ring of the magnetic member is made of a transparent material, and when the magnetic member lever module does not contact the magnetic ring abutment portion of the magnetic member, the light detection probe is located below or above the deviated magnetic member; The magnetic member lever module contacts the abutment portion and hinders the magnetic field switching rotating member from rotating. The optical detection probe is located directly below or directly above the magnetic member.

17. A heat preservation device, comprising a heating body for heating a microfluidic chip, characterized in that: The incubation body includes the magnetic field switching device as described in claim 9.

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