A pulling device for electrophoresis comb

By designing an electrophoresis comb pulling device consisting of a glass plate, a U-shaped side clamp and a lifting device, the problem of lane damage when the electrophoresis comb is pulled out is solved, the electrophoresis comb can be pulled out smoothly and vertically, and the accuracy of the experimental results is ensured.

CN111044594BActive Publication Date: 2025-09-16THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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Patent Information

Application Number
CN202010021272.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2020-01-09
Publication Date
2025-09-16
Estimated Expiration
2040-01-09

AI Technical Summary

Technical Problem

In the prior art, when the electrophoresis comb is pulled out, the swimming lane is easily damaged due to uneven force applied by both hands.

Method used

A pulling device consisting of two glass plates, a U-shaped side clamp and a lifting device was designed. A circular rod was inserted into the circular hole of the electrophoresis comb. Combined with the clamping part, the connecting part and the sliding part, the electrophoresis comb can be pulled out vertically to avoid damaging the swimming lane.

Benefits of technology

The electrophoresis comb can be pulled out smoothly and vertically, thus avoiding damage to the swimming lane caused by uneven force and improving the accuracy of the experimental results.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a pulling-out device for an electrophoresis comb. The key points of the technical solution are that the device comprises two glass plates and U-shaped side clamps arranged on the outside of the two glass plates, the two glass plates are arranged to form a holding cavity for accommodating gel, an electrophoresis comb is arranged in the holding cavity, and the electrophoresis comb can be pulled up, electrical connection plates are arranged on both sides of the holding cavity, an electrical connection head is arranged on the electrical connection plate, an electrode core is connected to the electrical connection head, the electrophoresis comb comprises a plurality of comb teeth and a connecting piece for connecting the plurality of comb teeth, a plurality of circular holes are provided on the connecting piece, and the circular holes are arranged and extended along the length direction of the connecting piece, a lifting device is further provided on the side of the electrical connection plate, a plurality of circular rods are provided inside the lifting device, the circular rods can be inserted into the circular holes to lift the electrophoresis comb vertically upward, and the pulling-out device can pull out the electrophoresis comb vertically upward, thereby avoiding damage to the swimming lane caused by uneven pulling out of the two sides of the electrophoresis comb.
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Description

Technical Field

[0001] The invention relates to a pulling device for an electrophoresis comb. Background Art

[0002] Electrophoresis technology utilizes the different migration rates of charged molecules in the sample to be separated, due to differences in their charge properties, size, and properties, under the action of an electric field, to separate, identify, or purify the sample. Most laboratories configure polyacrylamide gels based on actual needs, and the inherently shaped mold, the "electrophoresis comb," plays a key role in preparing polyacrylamide gels. In existing techniques, after the polyacrylamide gel is formed, the electrophoresis comb needs to be removed. This removal process is typically done manually by the experimenter. During this process, uneven force applied by the experimenter's hands often results in uneven removal of the comb, damaging the lanes and affecting the experimental results. Summary of the Invention

[0003] In view of the shortcomings of the prior art, the present invention aims to provide a pulling device for an electrophoresis comb, which can pull out the electrophoresis comb vertically upward to avoid damaging the swimming lane.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a pulling-up device for an electrophoresis comb, comprising two glass plates and U-shaped side clamps arranged on the outside of the two glass plates, the two glass plates forming a holding cavity for accommodating gel, an electrophoresis comb being arranged in the holding cavity, the electrophoresis comb being capable of being pulled up, electrical connection plates being arranged on both sides of the holding cavity, an electrical connection head being arranged on the electrical connection plate, an electrode core being connected to the electrical connection head, the electrophoresis comb comprising a plurality of comb teeth and a connecting piece for connecting the plurality of comb teeth, a plurality of circular holes being arranged on the connecting piece, the circular holes being arranged and extending along the length direction of the connecting piece, a lifting device being further provided on the side of the electrical connection plate, a plurality of circular rods being provided inside the lifting device, the circular rods being capable of being inserted into the circular holes to lift the electrophoresis comb upward.

[0005] Furthermore, the lifting device includes a clamping member, a connecting member and a sliding member. The sliding member is arranged on both sides of the connecting member, and the three form a "["-shaped structure. The circular rod is fixed on the connecting member, and the clamping member is sleeved on the electrical connection plate. The clamping member is clamped with the electrical connection plate. A through groove is provided on the clamping member, and the sliding member is slidably connected to the through groove.

[0006] Furthermore, the sliding member includes a first rod and a second rod, the second rod is sleeved in the first rod, one end of the first rod is fixedly connected to the connecting member, and the other end is slidably connected to the second rod, and the end of the second rod facing away from the first rod is slidably connected to the clamping member.

[0007] Furthermore, a connecting rod is provided on one end of the second rod away from the first rod, a ball is provided on the connecting rod, a spring is provided at the connection between the ball and the connecting rod, and a slot for the connecting rod to be inserted is provided on the clamping member, and the slot is connected to the through slot.

[0008] Furthermore, the through slot extends upward toward one end facing away from the electrical connection plate and conducts electricity to the electrical connection plate.

[0009] Beneficial effects of the present invention:

[0010] 1. The connection piece and the round rod are arranged to pull the electrophoresis comb upward vertically and smoothly, so as to prevent the operator from damaging the swimming lane due to uneven pulling speed of both sides of the electrophoresis comb due to uneven force of both hands.

[0011] 2. By setting the first rod and the second rod, the circular rod can be effectively inserted into the circular hole.

[0012] 3. Through the action of the connecting rod and the ball, the contact area between the second rod and the clamping member can be reduced, thereby reducing the friction force of the second rod during the sliding process. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the front structure of the electrophoresis comb pulling device;

[0014] Figure 2 A schematic diagram of the three-dimensional structure of the electrophoresis comb pulling device;

[0015] Figure 3 Schematic diagram of the connection structure between the connector and the circular rod;

[0016] Figure 4 Schematic diagram of the structure of the electrophoresis comb and the connecting plate;

[0017] Figure 5 Schematic diagram of the connection structure between the second rod and the clamping member.

[0018] Figure numerals: 1. accommodating chamber; 11. glass plate; 2. U-shaped side clamp; 3. electrophoresis comb; 31. comb teeth; 32. connecting piece; 33. circular hole; 4. electrical connection plate; 41. electrical connection head; 5. lifting device; 51. clamping member; 52. connecting member; 53. sliding member; 531. first rod; 532. second rod; 533. connecting rod; 534. ball; 535. spring; 536. slot; 6. circular rod; 7. through slot. DETAILED DESCRIPTION

[0019] The present invention will be described in further detail below with reference to the accompanying drawings and embodiments. Identical components are denoted by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "upper," and "lower" used in the following description refer to directions in the accompanying drawings, and the terms "bottom," "top," "inner," and "outer" refer to directions toward or away from the geometric center of a particular component, respectively.

[0020] Reference Figures 1 to 5 As shown, a pulling device for an electrophoresis comb in this embodiment includes two glass plates 11 and a U-shaped side clamp 2 arranged on the outside of the two glass plates 11, the two glass plates 11 are arranged to form a accommodating cavity 1 for accommodating a glue-making liquid, an electrophoresis comb 3 is arranged in the accommodating cavity 1, and the electrophoresis comb 3 can be pulled upwards, electrical connection plates 4 are provided on both sides of the accommodating cavity 1, an electrical connection head 41 is provided on the electrical connection plate 4, an electrode core is connected to the electrical connection head 41, the electrophoresis comb 3 includes a plurality of comb teeth 31 and a connecting piece 32 for connecting a plurality of comb teeth 31, a plurality of circular holes 33 are provided on the connecting piece 32, and the circular holes 33 are arranged and extended along the length direction of the connecting piece 32, a lifting device 5 is also provided on the side of the electrical connection plate 4, a plurality of circular rods 6 are provided inside the lifting device 5, and the circular rods 6 can be inserted into the circular holes 33 to lift the electrophoresis comb 3 upwards.

[0021] The above improvements are specifically as follows: the glass panel in this embodiment includes an inner thick glass plate and a thin glass plate, the thick glass plate is arranged on the outside, and the thin glass plate is arranged on the inside. The thin glass plate and the thick glass plate are collectively referred to as glass panels below. This structure is a prior art and will not be described in detail. The accommodating cavity 1 formed by the glass panel is used to accommodate the glue making liquid. The electrophoresis comb 3 is inserted, and the glue making liquid can form a swimming lane after being formed. U-shaped side clamps 2 are provided on the outside of the two glass plates 11. Through the setting of the U-shaped side clamps 2, the glass panels on both sides can be effectively tightened to ensure their sealing. Through the setting of the electrical connector 41 and the electrical connection core, electricity can be supplied to perform electrophoresis. Through the setting of the electrophoresis comb 3, the glue making liquid can be formed after being formed. The electrophoresis comb 3 is formed into a swimming lane, and a connecting piece 32 is mounted on the electrophoresis comb 3. The connecting piece 32 and the electrophoresis comb 3 are integrally formed, and a circular hole 33 is opened on the connecting piece 32. A lifting device 5 is provided on the side of the electrical connection plate 4. The function of the lifting device 5 is to lift the electrophoresis comb 3 upward steadily without damaging the swimming lane. A plurality of circular rods 6 are provided on the lifting device 5, and the circular rods 6 can be inserted into the circular holes 33. One circular rod 6 corresponds to one circular hole 33. When the electrophoresis comb 3 needs to be pulled out of the accommodating chamber 1, the lifting device 5 drives the circular rods 6 to be inserted into the circular holes 33, and then the electrophoresis comb 3 is lifted steadily. This is superior to the existing technology and can lift the electrophoresis comb 3 upward without damaging the swimming lane.

[0022] On the basis of the above embodiment, the lifting device 5 includes a clamping member 51, a connecting member 52 and a sliding member 53. The sliding member 53 is arranged on both sides of the connecting member 52, and the three form a "["-shaped structure. The circular rod 6 is fixed on the connecting member 52, and the clamping member 51 is sleeved on the electrical connection plate 4. The clamping member 51 is clamped with the electrical connection plate 4. A through groove 7 is provided on the clamping member 51, and the sliding member 53 is slidably connected to the through groove 7.

[0023] The above-mentioned improvements are specifically as follows: the lifting device 5 includes a clamping part 51, a connecting part 52 and a sliding part 53. The clamping part 51 is clamped on the electrical connection plate 4, and multiple round rods 6 are fixed on the connecting part 52. The sliding part 53 is fixed at both ends of the connecting part 52. A through groove 7 is opened on the clamping part 51, and the sliding part 53 can slide back and forth in the through groove 7. Through the coordinated arrangement of the clamping part 51, the connecting part 52 and the sliding part 53, the electrophoresis comb 3 can be effectively taken out of the glue making liquid without destroying the swimming lane formed by the electrophoresis comb 3.

[0024] Based on the above embodiment, the sliding member 53 includes a first rod 531 and a second rod 532. The second rod 532 is sleeved in the first rod 531. One end of the first rod 531 is fixedly connected to the connecting member 52, and the other end is slidingly connected to the second rod 532. The end of the second rod 532 facing away from the first rod 531 is slidingly connected to the clamping member 51.

[0025] The above-mentioned improvement is specifically as follows: the second rod 532 is sleeved in the first rod 531, and the first rod 531 and the second rod 532 are slidingly connected. When the electrophoresis comb 3 needs to be taken out of the glue-making liquid, the second rod 532 needs to be inserted into the through groove 7 first, and then the second rod 532 is pulled out from the first rod 531 so that the circular rod 6 can be inserted into the circular groove. Then, hold the connecting piece 32 with both hands and lift the electrophoresis comb 3 upward.

[0026] On the basis of the above embodiment, a connecting rod 533 is provided on the end of the second rod 532 away from the first rod 531, and a ball 534 is provided on the connecting rod 533. A spring 535 is provided at the connection between the ball 534 and the connecting rod 533, and a slot 536 for the connecting rod 533 to be inserted is provided on the clamping member 51, and the slot 536 is communicated with the through slot 7.

[0027] The above-mentioned improvements are specifically as follows: in order to reduce the sliding friction between the second rod 532 and the through slot 7, a connecting rod 533 is opened at the connection between the second rod 532 and the through slot 7, a ball 534 is provided on the connecting rod 533, and a slot 536 adapted to the connecting rod 533 is opened on the clamping member 51, so that the connecting rod 533 can be clamped into the slot 536. Through the arrangement of the connecting rod 533 and the slot 536, the contact area between the second rod 532 and the clamping member 51 can be effectively reduced, and the friction of the second rod 532 during movement can be effectively reduced.

[0028] On the basis of the above embodiment, the through slot 7 extends upward toward the end facing away from the electrical connection board 4 and conducts electricity to the electrical connection board 4 .

[0029] The above improvement is specifically as follows: by extending the through slot 7 upward toward the end facing away from the electrical connection plate 4 and conducting the electrical connection plate 4, the lifting device 5 can be removed to avoid it taking up space when it is not needed.

[0030] The above description is merely a preferred embodiment of the present invention. The scope of protection of the present invention is not limited to the above embodiment. All technical solutions based on the concept of the present invention are within the scope of protection of the present invention. It should be noted that for those skilled in the art, various improvements and modifications that do not depart from the principles of the present invention should also be considered within the scope of protection of the present invention.

Claims

1. A device for pulling out an electrophoresis comb, comprising two glass plates (11) and U-shaped side clamps (2) arranged on the outside of the two glass plates (11), wherein the two glass plates (11) enclose a receiving cavity (1) for receiving a gel, wherein an electrophoresis comb (3) is arranged in the receiving cavity (1), and the electrophoresis comb (3) can be pulled up, wherein electrical connection plates (4) are arranged on both sides of the receiving cavity (1), wherein electrical connection heads (41) are arranged on the electrical connection plates (4), and wherein an electrode core is connected to the electrical connection heads (41), wherein the device is characterized in that: The electrophoresis comb (3) comprises a plurality of comb teeth (31) and a connecting piece (32) for connecting the plurality of comb teeth (31); the connecting piece (32) is provided with a plurality of circular holes (33); the circular holes (33) are arranged along the length direction of the connecting piece (32); a lifting device (5) is further provided on the side of the electrical connection plate (4); a plurality of circular rods (6) are provided inside the lifting device (5); the circular rods (6) can be inserted into the circular holes (33) to lift the electrophoresis comb (3) vertically upward; The lifting device (5) includes a clamping member (51), a connecting member (52) and a sliding member (53), wherein the sliding member (53) is arranged on both sides of the connecting member (52), and the three members form a "["-shaped structure, wherein the circular rod (6) is fixed on the connecting member (52), the clamping member (51) is sleeved on the electrical connection plate (4), the clamping member (51) is clamped with the electrical connection plate (4), a through slot (7) is provided on the clamping member (51), and the sliding member (53) is slidably connected to the through slot (7); The sliding member (53) includes a first rod (531) and a second rod (532), wherein the second rod (532) is sleeved in the first rod (531), one end of the first rod (531) is fixedly connected to the connecting member (52), and the other end is slidably connected to the second rod (532), and the end of the second rod (532) facing away from the first rod (531) is slidably connected to the clamping member (51); The through slot (7) extends upwards towards one end facing away from the electrical connection plate (4) and conducts electricity to the electrical connection plate (4).

Citation Information

Patent Citations

  • SDS supplementary instrument of pulling out of PAGE gel application of sample hole comb

    CN206411060U

  • Pulling device of electrophoretic comb

    CN211905196U