One-time collection device for isolating Bursaphelenchus xylophilus

Abstract

The present invention is a disposable device for collecting and separating Bursaphelenchus xylophilus, which is provided with a collection bottle, a bottle cap, funnel A, filter paper and funnel B; the collection bottle mouth of the collection bottle is internally provided with funnel A, and filter paper and funnel B are sequentially arranged in funnel A; a bottle cap convex ring is arranged inside the bottle cap, and the bottle cap and the collection bottle mouth of the collection bottle are provided with a threaded fit connection. A number of 1-3 circles of filter holes are evenly arranged on the funnel wall at the funnel mouth end of funnel A, the funnel wall and the funnel bottom are integrated, and the funnel mouth of funnel A is set as a vertical convex edge or a flat convex edge, the vertical convex edge is matched with the inner diameter of the collection bottle mouth, and the outer diameter of the flat convex edge is matched with the outer diameter of the collection bottle mouth. The filtering and separation effect of this separation device for collecting Bursaphelenchus xylophilus is good, the operation is convenient, the volume is small, and it is easy to carry. When working in the wild, it can quickly separate Bursaphelenchus xylophilus to ensure the reliability of the results, and it is suitable for medium and small forest pest control stations, etc.

Description

Technical Field

[0001] The present invention relates to biological control technology and the technical field of isolation and collection of Bursaphelenchus xylophilus, and specifically relates to a disposable Bursaphelenchus xylophilus isolation device for filtering, separating and collecting Bursaphelenchus xylophilus samples. Background Art

[0002] Bursaphelenchus xylophilus is the pathogen that causes the epidemic of pine wilt disease in pine trees. Diseased pine trees will die rapidly, with high transmissibility, and will cause a large number of pine trees to wither and die in a short time, which is called "the cancer of pine trees". Therefore, rapid quarantine of Bursaphelenchus xylophilus is an important preventive measure. At present, relevant quarantine departments generally adopt indoor on-site inspection, and the Bellman funnel method is used to separate Bursaphelenchus xylophilus in the laboratory. When conducting batch detection, a large number of funnel devices and special sites are required, and due to the complex structure, problems will be encountered during operation.

[0003] The existing separation devices cannot operate in the wild, and the operation is complex. Each individual filtering device needs to be specially cleaned. Incomplete cleaning will cause cross-infection of samples. In view of the above situation, the purpose of the present invention is to provide a portable disposable Bursaphelenchus xylophilus isolation device. Summary of the Invention

[0004] The purpose of the present invention is to provide a disposable Bursaphelenchus xylophilus isolation device that is convenient, practical, economical, can separate nematodes by sampling anywhere in the wild, and can prevent the possibility of cross-contamination of samples.

[0005] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0006] A disposable Bursaphelenchus xylophilus isolation device is provided with a collection bottle, a collection bottle mouth, a bottle cap, funnel A, a filter paper and funnel B; the collection bottle mouth of the collection bottle is internally provided with funnel A, and a filter paper and funnel B are sequentially arranged in funnel A; a bottle cap convex ring is arranged inside the bottle cap, and the bottle cap and the collection bottle mouth of the collection bottle are provided with a threaded fit connection.

[0007] Further, the funnel wall at the funnel mouth end of funnel A is evenly provided with several 1-3 circles of filter holes, the funnel wall and the funnel bottom are integrally formed, the funnel mouth of funnel A is provided with a funnel convex edge, and the funnel convex edge is set as a vertical convex edge or a flat convex edge. The vertical convex edge of funnel A with a vertical convex edge matches the inner diameter of the collection bottle mouth, and the outer diameter of the flat convex edge of funnel A with a flat convex edge matches the outer diameter of the collection bottle mouth.

[0008] Further, the outer diameter of the collection bottle mouth is 25-30 millimeters.

[0009] Further, the diameter of the filter hole is 1-2 millimeters.

[0010] Further, the funnel A is injection-molded or formed by pressing a mold using a degradable composite material.

[0011] Further, the depth of the funnel A is 7 - 10 millimeters.

[0012] Further, the collection bottle and the bottle cap are either commercially available small mineral water bottles.

[0013] Further, the setting of the funnel B is the same as that of the funnel A. The difference in the setting of the filter paper from that of the funnels A and B is that it has no filter holes.

[0014] Further, the bottle cap convex ring is either a bottle cap convex platform, and the height of the convex ring and the convex platform is 3 - 5 millimeters.

[0015] The present invention has at least the following beneficial effects:

[0016] 1. Each disposable pinewood nematode separation device is an independent device, which is formed by connecting a bottle cap, a funnel, a filter paper sheet, and a funnel to a collection bottle from bottom to top. There are mutually fitting screw threads on the inner side of the bottle cap and the edge of the collection bottle mouth, and the space of the bottle cap collects the sample extraction liquid.

[0017] 2. The disposable pinewood nematode separation device utilizes the fluidity of the liquid for soaking the sample. With the mouth of the separation device facing downwards, by covering the filter paper sheet on the surface of the funnel, the extraction liquid is collected inside the bottle cap. Then, with the mouth of the separation device facing upwards, the extraction liquid falls to the bottom of the funnel, achieving the effects of quickly removing other impurities in the sample, having good filtering, separating, and collecting effects on pinewood nematodes, and being convenient to operate. It can achieve batch separation according to the number of samples, avoiding waste of resources.

[0018] 3. The disposable pinewood nematode separation device is small in volume and easy to carry. When working in the wild, it can timely extract samples and quickly separate pinewood nematodes, ensuring the reliability of the results.

[0019] 4. The disposable pinewood nematode separation device is more convenient for emergency forest epidemic situations, facilitating the quick filtering and separation of pinewood nematodes, and is suitable for small and medium-sized forest pest control stations, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 : It is a schematic diagram of the local cross-section structure of the built-in funnel of this device.

[0021] Figure 2 : It is a schematic diagram of the local cross-section structure of the pressing bottle mouth funnel of this device.

[0022] Figure 3 : It is a schematic diagram of the cross-section structure of the funnel with an arc bottom.

[0023] Figure 4 : It is a schematic diagram of the cross-section structure of the funnel with a flat bottom.

[0024] Figure 5 : It is the top view of the funnel.

[0025] Identifications in the figure: 1 - collection bottle, 11 - collection bottle mouth, 2 - bottle cap, 3 - funnel A, 31 - filtering holes, 32 - funnel wall, 33 - funnel bottom, 34 - funnel mouth, 35 - funnel convex edge, 36 - bottle cap convex ring, 4 - filter paper, 5 - funnel B. Specific embodiments

[0026] The following further describes in detail the specific embodiments of the present invention with reference to the accompanying drawings. The following embodiments are only one of the more preferred specific ways of the present invention and are not intended to limit the scope of the present invention. Any ordinary variations and substitutions made by technicians in the field within the scope of this technical solution shall fall within the scope of protection of the present invention.

[0027] Embodiment 1

[0028] As shown in Figure 1 and Figure 3 , a disposable device for collecting pine wood nematodes is provided, which includes a collection bottle 1, a collection bottle mouth 11, a bottle cap 2, a funnel A 3, a filter paper 4 and a funnel B 5; the collection bottle mouth 11 of the collection bottle 1 is internally provided with a funnel A 3, and a filter paper 4 and a funnel B 5 are sequentially arranged in the funnel A 3; a bottle cap convex ring 36 is arranged inside the bottle cap 2, and the bottle cap 2 and the collection bottle mouth 11 of the collection bottle 1 are provided with a threaded fit connection.

[0029] Furthermore, the funnel wall 32 at one end of the funnel mouth 34 of the funnel A 3 is evenly provided with several filtering holes 31 in 2 circles, the funnel wall 32 and the funnel bottom 33 are integrally formed, the funnel mouth 34 of the funnel A 3 is provided with a funnel convex edge 35, and the funnel convex edge 35 is set as a chamfered vertical convex edge, and the vertical convex edge of the funnel A 3 is matched with the inner diameter of the collection bottle mouth 11.

[0030] Furthermore, the outer diameter of the collection bottle mouth 11 is 25 mm.

[0031] Furthermore, the diameter of the filtering holes 31 is 1 mm.

[0032] Furthermore, the funnel A 3 is injection molded.

[0033] Furthermore, the depth of the funnel A 3 is 7 mm.

[0034] Furthermore, the setting of the funnel B 5 is the same as that of the funnel A 3, and the difference between the setting of the filter paper 4 and the settings of the funnel A 3 and the funnel B 5 is that there are no filtering holes 31.

[0035] Furthermore, the height of the bottle cap convex ring 36 is 3 mm.

[0036] Embodiment 2

[0037] As Figure 1 and Figure 3 shown, a disposable pine wood nematode separation and collection device is provided with a collection bottle 1, a collection bottle mouth 11, a bottle cap 2, a funnel A 3, a filter paper 4 and a funnel B 5; the funnel A 3 is disposed inside the collection bottle mouth 11 of the collection bottle 1, and the filter paper 4 and the funnel B 5 are sequentially disposed inside the funnel A 3; a bottle cap convex ring 36 is disposed inside the bottle cap 2, and the bottle cap 2 and the collection bottle mouth 11 of the collection bottle 1 are provided with a threaded engagement connection.

[0038] Further, a plurality of filter holes 31 are evenly disposed in 3 circles on the funnel wall 32 at one end of the funnel mouth 34 of the funnel A 3, the funnel wall 32 and the funnel bottom 33 are integrally formed, a funnel convex edge 35 is disposed at the funnel mouth 34 of the funnel A 3, and the funnel convex edge 35 is disposed as a vertical convex edge, and the vertical convex edge of the funnel A 3 with the vertical convex edge matches the inner diameter of the collection bottle mouth 11.

[0039] Further, the outer diameter of the collection bottle mouth 11 is 30 millimeters.

[0040] Further, the diameter of the filter hole 31 is 2 millimeters.

[0041] Further, the funnel A 3 is formed by pressing a degradable composite material.

[0042] Further, the depth of the funnel A 3 is 10 millimeters.

[0043] Further, the setting of the funnel B 5 is the same as the setting of the funnel A 3, and the difference between the setting of the filter paper 4 and the settings of the funnel A 3 and the funnel B 5 is that there are no filter holes 31.

[0044] Further, the height of the bottle cap convex ring 36 is 4 millimeters.

[0045] Example 3

[0046] As Figure 2 and Figure 4 shown, a disposable pine wood nematode separation and collection device is provided with a collection bottle 1, a collection bottle mouth 11, a bottle cap 2, a funnel A 3, a filter paper 4 and a funnel B 5; the funnel A 3 is disposed inside the collection bottle mouth 11 of the collection bottle 1, and the filter paper 4 and the funnel B 5 are sequentially disposed inside the funnel A 3; a bottle cap convex ring 36 is disposed inside the bottle cap 2, and the bottle cap 2 and the collection bottle mouth 11 of the collection bottle 1 are provided with a threaded engagement connection.

[0047] Further, a number of filter holes 31 are evenly arranged in two circles on the funnel wall 32 at one end of the funnel mouth 34 of the funnel A3. The funnel wall 32 and the funnel bottom 33 are integrally formed. A funnel convex edge 35 is provided at the funnel mouth 34 of the funnel A3. The funnel convex edge 35 is set as a flat convex edge, and the outer diameter of the flat convex edge matches the outer diameter of the collection bottle mouth 11.

[0048] Further, the outer diameter of the collection bottle mouth 11 is 30 millimeters.

[0049] Further, the diameter of the filter hole 31 is 1.5 millimeters.

[0050] Further, the funnel A3 is formed by pressing a degradable composite material.

[0051] Further, the depth of the funnel A3 is 10 millimeters.

[0052] Further, the setting of the funnel B5 is the same as that of the funnel A3. The difference between the setting of the filter paper 4 and the settings of the funnel A3 and the funnel B5 is that there are no filter holes 31.

[0053] Further, the height of the trapezoidal convex ring of the bottle cap convex ring 36 is 5 millimeters.

[0054] Application method of the separation device of the present invention:

[0055] During use, first moisten the filter paper with the extraction solution and adsorb it in the funnel A. Put all the samples and the extraction solution into the connection bottle, snap the funnel A with the adsorbed filter paper onto the connection bottle mouth, then place the funnel B, rotate the connection bottle cap, use the bottle cap convex ring to push the funnel 1 millimeter into the connection bottle mouth, invert the separation device with the bottle mouth downwards, release the filter pine wood nematode extraction solution to store the bottle cap, after standing for 6 hours, place the separation device with the bottle mouth upwards, let the sample extraction solution flow back into the funnel, open the connection bottle cap, and collect the sample extraction solution in the funnel for the next detection.

Claims

1. An application method of a disposable device for collecting Bursaphelenchus xylophilus, characterized in that: The separation device it adopts is provided with a collection bottle (1), a bottle cap (2), a funnel A (3), a filter paper (4) and a funnel B (5); the collection bottle mouth (11) of the collection bottle (1) is internally provided with the funnel A (3), and the filter paper (4) and the funnel B (5) are sequentially arranged in the funnel A (3); a bottle cap convex ring (36) is arranged inside the bottle cap (2), and the bottle cap (2) and the collection bottle mouth (11) of the collection bottle (1) are arranged in a threaded fit connection; the funnel wall (32) at one end of the funnel mouth (34) of the funnel A (3) is evenly provided with several 1-3 circles of filter holes (31), the funnel wall (32) and the funnel bottom (33) are integrally formed, a funnel convex edge (35) is arranged at the funnel mouth (34) of the funnel A (3), and the funnel convex edge (35) is set as a vertical convex edge or a flat convex edge. The vertical convex edge of the funnel A (3) with the vertical convex edge is matched with the inner diameter of the collection bottle mouth (11), and the outer diameter of the flat convex edge of the funnel A (3) with the flat convex edge is matched with the outer diameter of the collection bottle mouth (11); the setting of the funnel B (5) is the same as that of the funnel A (3), and the difference between the setting of the filter paper (4) and the settings of the funnel A (3) and the funnel B (5) is that there are no filter holes (31); When in use, first moisten the filter paper sheet with the extraction solution and adsorb it in the funnel A, then put all the samples and the extraction solution into the collection bottle, insert the funnel A with the adsorbed filter paper sheet into the connecting bottle mouth, then put in the funnel B, rotate the connecting bottle cap, and use the bottle cap convex ring to push the funnel 1 mm into the connecting bottle mouth. Invert the bottle mouth of the separation device downward, release the filtered pine wood nematode extraction solution and store it in the bottle cap. After standing for 6 hours, place the bottle mouth of the separation device upward, let the sample extraction solution flow back into the funnel, open the connecting bottle cap, and collect the sample extraction solution in the funnel for the next detection.

2. The application method of the disposable device for collecting Bursaphelenchus xylophilus isolates according to claim 1, characterized in that: The outer diameter of the collection bottle mouth (11) is 25-30 mm.

3. The application method of the disposable pine wood nematode isolation device according to claim 2, characterized in that: The diameter of the filter hole (31) is 1-2 mm.

4. The application method of the disposable pine wood nematode isolation device according to claim 1 or 2, characterized in that: The funnel A (3) is injection molded or formed by pressing with a degradable composite material.

5. The application method of the disposable collection device for pine wood nematode isolation according to claim 1 or 2, characterized in that: The depth of the funnel A (3) is 7-10 mm.

6. The application method of the disposable pine wood nematode isolation device according to claim 1, characterized in that: The collection bottle (1) and the bottle cap (2) are commercially available small mineral water bottles.

7. The application method of the disposable pine wood nematode isolation device according to claim 1, characterized in that: The bottle cap convex ring (36) is a bottle cap convex platform, and the height of the convex ring and the convex platform is 3-5 mm.

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