Subculture breeding method of thorn-free zanthoxylum bungeanum maxim tissue culture seedlings

Abstract

The invention discloses a subculture breeding method of thorn-free zanthoxylum bungeanum maxim tissue culture seedlings, and belongs to the technical field of vegetative propagation of commercial crops. The subculture breeding method specifically comprises the following steps: primary culture, cutting and subculture multiplication culture, wherein the subculture multiplication culture is finisheduntil PPM use amount in a culture medium is reduced to 0, and the number of generations of the subculture multiplication culture is not less than 4. According to the subculture breeding method of thethorn-free zanthoxylum bungeanum maxim tissue culture seedlings, a technical system of subculture of the thorn-free zanthoxylum bungeanum maxim tissue culture seedlings is established through optimizing culture conditions, properly adjusting the composition of components of the culture medium according to the number of times of the subculture and comprehensively researching factors including a special cutting process and the like, so that subculture multiplication of stem segments of primary culture of the thorn-free zanthoxylum bungeanum maxim is rapidly and stably realized, and furthermore,large-scale breeding is realized in a short time; and the subculture breeding method has great practical value in breeding production of thorn-free zanthoxylum bungeanum maxim.

Description

technical field

[0001] The invention belongs to the technical field of asexual propagation of economic crops, and in particular relates to a method for subculture of prickly prickly ash tissue culture seedlings. Background technique

[0002] Zanthoxylum is a spice plant of Rutaceae Zanthoxylum Linn. It has a long history of cultivation in my country. It is not only a spice plant but also a traditional medicinal plant. Cultivated Zanthoxylum has high economic value and soil and water conservation benefits. Zanthoxylum bungeanum is distributed in the provinces of the Yellow River, Yangtze River, and Pearl River basins, as well as Yunnan, Guizhou, and Tibet in China. According to the type of commodity, it can be divided into northern red pepper, southern green pepper and fragrant pepper produced in Shandong.

[0003] my country has planted about 2 million hectares of Zanthoxylum bungeanum 2 , with an annual output value of about 60 billion yuan, Zanthoxylum bungeanum has becom...

Examples

Embodiment 1

[0035] A method for subculture of prickly prickly ash tissue culture seedlings, comprising the following steps:

[0036] S1. Primary culture: put the sprouted stems of prickly prickly ash in the culture medium, and culture them in a dark environment for 8-9 days, then place them under the condition of light intensity of 1000lux for 7-8 days, and then increase the light intensity to 2000lux Cultivate for 13 to 14 days until the sprouted stems of prickly prickly ash grow to a height of 3 to 4 cm, with 3 to 4 internodes and no less than 2 axillary buds. figure 1 shown;

[0037] S2. Cutting: place the sprouted stems of the prickly prickly ash through the primary culture in the aseptic inoculation tray on the ultra-clean workbench, and use sterilized tools to immerse the base of the sprouted stems of the prickly prickly ash into one end of the culture medium Remove the tissue to expose the fresh tissue, then cut the terminal buds into 1-2cm long stems with 2-4 leaves, and cut the re...

technical field

[0001] The invention belongs to the technical field of asexual propagation of economic crops, and in particular relates to a method for subculture of prickly prickly ash tissue culture seedlings. Background technique

[0002] Zanthoxylum is a spice plant of Rutaceae Zanthoxylum Linn. It has a long history of cultivation in my country. It is not only a spice plant but also a traditional medicinal plant. Cultivated Zanthoxylum has high economic value and soil and water conservation benefits. Zanthoxylum bungeanum is distributed in the provinces of the Yellow River, Yangtze River, and Pearl River basins, as well as Yunnan, Guizhou, and Tibet in China. According to the type of commodity, it can be divided into northern red pepper, southern green pepper and fragrant pepper produced in Shandong.

[0003] my country has planted about 2 million hectares of Zanthoxylum bungeanum 2 , with an annual output value of about 60 billion yuan, Zanthoxylum bungeanum has becom...

Examples

Embodiment 1

[0035] A method for subculture of prickly prickly ash tissue culture seedlings, comprising the following steps:

[0036] S1. Primary culture: put the sprouted stems of prickly prickly ash in the culture medium, and culture them in a dark environment for 8-9 days, then place them under the condition of light intensity of 1000lux for 7-8 days, and then increase the light intensity to 2000lux Cultivate for 13 to 14 days until the sprouted stems of prickly prickly ash grow to a height of 3 to 4 cm, with 3 to 4 internodes and no less than 2 axillary buds. figure 1 shown;

[0037] S2. Cutting: place the sprouted stems of the prickly prickly ash through the primary culture in the aseptic inoculation tray on the ultra-clean workbench, and use sterilized tools to immerse the base of the sprouted stems of the prickly prickly ash into one end of the culture medium Remove the tissue to expose the fresh tissue, then cut the terminal buds into 1-2cm long stems with 2-4 leaves, and cut the re...

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