Immunoassay Test Kits

Abstract

The present application provides an immunoassay reagent card, including a housing, a test strip, a sealing plug and a reagent kit structure, wherein the test strip is fixed in a closed cavity of the housing, a sample addition port and a visual window are provided on the housing, and the reagent kit structure includes a sleeve fixed at one end on the housing and a reagent cup slidably mounted in the sleeve, and a puncture piece for puncturing the closed end of the reagent cup is provided in the cavity. The immunoassay reagent card provided by the present application, when performing an immunoassay, the sample is released onto the test strip through the sample addition port, and the sample addition port is sealed with a sealing plug to avoid aerosol exchange, which can effectively prevent infection to the operator while improving the accuracy of the detection and analysis, and will not cause pollution to the environment. When releasing the reagent onto the test strip, it is only necessary to use an external force to press the reagent cup in the sleeve toward the cavity of the housing, and the puncture piece can puncture the closed end of the reagent cup, and the reagent in the reagent cup can be quickly released onto the test strip, which is easy to operate and has high detection efficiency.

Description

Technical Field

[0001] The present application belongs to the field of immunochromatography technology, and more specifically, relates to an immunoassay reagent card. Background Art

[0002] Immunochromatography technology has the advantages of fast detection speed, simple operation and low cost, and has become a commonly used technical means in food safety, medical and other detection. When the current immunoassay reagent card performs biochemical immunoassay on the sample, it usually uses a pipette gun or other pipetting instruments to pipette and release the sample and reagent into the sample injection port respectively. After the sample and reagent in the sample injection port are mixed, the chromatography reaction is carried out through the chromatography paper strip.

[0003] However, since the open sample port is directly connected to the external environment, especially in the process of high-sensitivity biochemical immunoassay, not only is aerosol exchange prone to occur, causing external pollutants to interfere with the detection and analysis, resulting in inaccurate test results; but also the reagents (such as biochemical reagents that are prone to biochemical hazards) contain volatile harmful substances that will pollute the external environment. At the same time, if the sample contains substances that can be transmitted through aerosols (for example, highly contagious pathogens, viruses, etc.), there is also a risk of infecting the operator while waiting for chromatography and during the transportation of the reagent card after use. Summary of the invention

[0004] The purpose of the embodiments of the present application is to provide an immunoassay reagent card to solve the problem that the immunoassay reagent card in the prior art is not only prone to aerosol exchange during biochemical immunoassay, resulting in inaccurate test results, but also has the risk of infecting the operator and polluting the environment.

[0005] To achieve the above purpose, the technical solution adopted in this application is: to provide an immunoassay reagent card, including:

[0006] A shell body, provided with a sealed cavity and a sample adding port communicated with the cavity;

[0007] A test strip is placed and fixed in the cavity;

[0008] A sealing plug, used to seal the sample addition port; and

[0009] A reagent kit structure, used for pre-sealing and packaging reagents, the reagent kit structure comprising a sleeve with one end fixed to the housing and a reagent cup slidably mounted in the sleeve;

[0010] The shell is provided with a visual window for observing the test status of the test strip, and the shell is also provided with an opening for the closed end of the reagent cup to pass through, the opening is connected to the cavity, and a piercing member for piercing the closed end of the reagent cup is correspondingly provided in the cavity.

[0011] Optionally, a storage pool for accommodating reagents is provided at a position in the cavity corresponding to the opening, the opening is communicated with the storage pool, and one end of the test strip is placed in the storage pool.

[0012] Optionally, a slot for holding the test strip is provided on a side wall of the storage tank, and one end of the test strip passes through the slot and extends into the storage tank.

[0013] Optionally, a positioning groove for accommodating and positioning the test strip is provided at the bottom of the storage tank.

[0014] Optionally, the piercing member is a column with one end fixed in the cavity, and the other end of the column extends into the opening.

[0015] Optionally, the diameter of the column gradually decreases from an end away from the reagent cup to an end close to the reagent cup.

[0016] Optionally, the reagent kit structure also includes a sliding structure for guiding the reagent cup to move in the sleeve, the sliding structure includes a slider and a slide groove slidably matched with the slider, the slider is convexly arranged on the outer wall of the reagent cup, and the slide groove is concavely arranged on the inner wall of the sleeve.

[0017] Optionally, the shell body includes a lower shell and an upper cover detachably connected to the lower shell, the sample loading port, the visual window and the opening are respectively arranged on the upper cover, one end of the sleeve is arranged around the opening and fixed on the upper cover, and the upper cover is sealed and connected to the lower shell to form the closed cavity.

[0018] Optionally, the reagent kit structure also includes a piston structure for pushing the reagent cup toward the cavity, the piston structure is slidably installed in the sleeve, the end of the reagent cup facing away from the closed end is an open end, and the end of the piston structure is sealed on the open end of the reagent cup.

[0019] Optionally, the piston structure includes a piston body and a push rod connected to the piston body, the end of the piston body away from the push rod is sealed on the open end of the reagent cup, and the end of the push rod away from the piston body protrudes from the port at the other end of the sleeve.

[0020] Compared with the prior art, the above one or more technical solutions in the embodiments of the present application have at least one of the following technical effects:

[0021] The immunoassay reagent card of the present application, in the process of biochemical immunoassay, releases the sample to the test strip in the closed cavity of the shell through the sample adding port, and uses the sealing plug to seal the sample adding port, so as to avoid the sample adding port being directly connected to the external environment and aerosol exchange, thereby avoiding the interference of external pollutants on the detection and analysis, improving the accuracy of the detection and analysis, and preventing the highly infectious bacteria, viruses, etc. contained in the sample from being transmitted through aerosols, which can effectively prevent the operator from being infected, and has high safety and reliability. When releasing the reagent on the test strip in the closed cavity of the shell, it is only necessary to press the reagent cup in the sleeve toward the cavity of the shell, and the puncture piece in the cavity can puncture the closed end of the reagent cup, and the reagent encapsulated in the reagent cup can be quickly released to the test strip, which is simple and convenient to operate and improves the detection efficiency. In addition, the test kit structure is a closed space, which can prevent the volatile harmful substances in the reagent from polluting the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0023] Figure 1 A schematic diagram of the three-dimensional structure of an immunoassay reagent card provided in an embodiment of the present application;

[0024] Figure 2 A schematic diagram of the top view of the immunoassay reagent card provided in an embodiment of the present application;

[0025] Figure 3 for Figure 2 Schematic diagram of the cross-sectional structure along the AA line;

[0026] Figure 4 for Figure 3 A schematic diagram of a partially enlarged structure in FIG.

[0027] Figure 5 An exploded view of the immunoassay reagent card provided in an embodiment of the present application;

[0028] Figure 6 for Figure 5 A schematic diagram of a partially enlarged structure in FIG.

[0029] Figure 7 A schematic diagram of the three-dimensional structure of the sleeve assembled on the upper cover provided in the embodiment of the present application;

[0030] Figure 8 for Figure 7 A schematic diagram of a partially enlarged structure in FIG.

[0031] Fig. 9 A schematic diagram of the three-dimensional structure of a reagent cup provided in an embodiment of the present application;

[0032] Fig.10 A schematic diagram of the three-dimensional structure of the piston structure provided in an embodiment of the present application.

[0033] Among them, the reference numerals in the figure are:

[0034] 1-Test strips;

[0035] 2-shell; 21-lower shell; 211-insertion rod; 22-upper cover; 221-sample adding port; 222-visible window; 223-opening; 224-insertion hole; 23-cavity;

[0036] 3- Sealing plug;

[0037] 4-reagent box structure; 41-sleeve; 411-slideway; 42-reagent cup; 421-open end; 422-closed end; 423-slider; 43-piston structure; 431-piston body; 432-push rod; 433-ring groove;

[0038] 5-Piercing member;

[0039] 6- Sealing ring;

[0040] 7-card position structure; 71-plywood; 72-card position;

[0041] 8-pressure column;

[0042] 9-storage tank; 91-bayonet; 92-positioning slot. DETAILED DESCRIPTION

[0043] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0044] It should be noted that when an element is referred to as being "connected to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0045] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0046] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0047] Please also read Figures 1 to 3 and Figure 4 , the immunoassay reagent card provided in the embodiment of the present application is now described. The immunoassay reagent card provided in the embodiment of the present application can be used for testing harmful substances such as highly infectious pathogens, viruses and highly volatile chemical reagents, and can avoid harm to the human body during the transportation and waste disposal of the reagent card. The immunoassay reagent card provided in the embodiment of the present application includes a test strip 1, a shell 2, a sealing plug 3 and a reagent kit structure 4, and a closed cavity 23 is provided inside the shell 2, and the test strip 1 is placed and fixed in the cavity 23. For further reference Figure 4 The housing 2 is provided with a sample adding port 221 communicating with the cavity 23, and the sealing plug 3 is used to seal the sample adding port 221 to prevent aerosol exchange from occurring during the biochemical immunoassay. Figure 2 The housing 2 is provided with a closed visual window 222, which is convenient for the user to observe the test status of the test strip 1 through the visual window 222. The reagent kit structure 4 is used to pre-seal and package the reagents, reduce the contact between the internal system of the immunoassay reagent card and the external environment, and prevent the volatile harmful substances in the reagents from polluting the external environment. Figure 4 The reagent kit structure 4 includes a sleeve 41 fixed at one end on the housing 2 and a reagent cup 42 slidably mounted in the sleeve 41. The housing 2 is also provided with an opening 223 for the closed end 422 of the reagent cup 42 to pass through. The opening 223 is connected to the cavity 23 so that the piston structure 43 can push the closed end 422 of the reagent cup 42 into the cavity 23. For further reference, Figure 4 and Figure 6, a piercing member 5 for piercing the closed end 422 of the reagent cup 42 is correspondingly provided in the cavity 23. When in use, an external force is used to push the reagent cup 42 toward the cavity 23 of the housing 2, so that the closed end 422 of the reagent cup 42 moves and is placed in the cavity 23, and the closed end 422 of the reagent cup 42 is pierced by the piercing member 5 in the cavity 23, so that the reagent sealed in the reagent cup 42 flows into the cavity 23.

[0048] Compared with the prior art, the immunoassay reagent card provided by the present application, in the process of biochemical immunoassay, releases the sample to the test strip 1 in the closed cavity 23 of the housing 2 through the sample adding port 221, and uses the sealing plug 3 to seal the sample adding port 221, so as to avoid the sample adding port 221 from being directly connected to the external environment and causing aerosol exchange, thereby avoiding the interference of external pollutants on the detection and analysis, improving the accuracy of the detection and analysis, and preventing the highly infectious bacteria and viruses contained in the sample from being transmitted through aerosols, which can effectively prevent the operator from being infected, and has high safety and reliability. When releasing the reagent to the test strip 1 in the closed cavity 23 of the housing 2, it is only necessary to press the reagent cup 42 in the sleeve 41 toward the cavity of the housing, and the piercing member 5 in the cavity 23 can pierce the closed end 422 of the reagent cup 42, and the reagent encapsulated in the reagent cup 42 can be quickly released to the test strip 1, which is simple and convenient to operate and improves the detection efficiency. Moreover, the reagent box structure 4 is a closed space, which can prevent the volatile harmful substances in the reagent from polluting the environment and affecting human health. In addition, after the test is completed, the sample and the reagent are sealed and stored in the closed cavity 23 of the shell 2, which reduces the difficulty of collecting and processing the waste of subsequent tests, and avoids the biological infection hazard to the operator during the collection, transportation and processing of the waste.

[0049] It can be understood that in one embodiment, the closed end 422 of the reagent cup 42 can be a plastic part that is integrally formed with the reagent cup 42 and is easily pierced or punctured by the puncture member 5. In another embodiment, the closed end 422 of the reagent cup 42 can also be a sealing sheet or sealing film such as aluminum foil or plastic film that is closed on the corresponding port of the reagent cup 42, and the sealing sheet or sealing film is easily pierced or punctured by the puncture member 5. In another embodiment, the closed end 422 of the reagent cup 42 can also be a soft plastic part made of materials such as silicone or rubber and easily pierced or punctured by the puncture member 5. In addition, considering that a thick soft plastic part is generally difficult to be quickly punctured by the puncture member 5, so that the reagent in the reagent cup 42 cannot be quickly released into the cavity 23 of the housing 2, it is also possible to provide a liquid outlet on the end face of the closed end 422 of the reagent cup 42, and set a sealing sheet that can be punctured by the puncture member 5 in the liquid outlet. Correspondingly, the puncture member 5 can be a column or rod with a pointed tip at one end, or a puncture strip with a knife-shaped edge at one end, etc. The specific structure of the puncture member 5 is not limited here and can be selected and set according to actual needs.

[0050] In another embodiment of the present application, please refer to Figure 5 , Figures 7 and 8 The shell body 2 includes a lower shell 21 and an upper cover 22 detachably connected to the lower shell 21. The sample loading port 221, the visual window 222 and the opening 223 are respectively arranged on the upper cover 22. One end of the sleeve 41 is arranged around the opening 223 and fixed on the upper cover 22. The upper cover 22 is sealed and connected to the lower shell 21 to form a closed cavity 23.

[0051] In this embodiment, the housing 2 is configured as a lower shell 21 and an upper cover 22 detachably connected to the lower shell 21, wherein the upper cover 22 and the lower shell 21 are combined together in a sealed bonding form, so that the upper cover 22 and the lower shell 21 are sealed and connected to form a closed cavity 23, which is convenient for installing the test strip 1 and inserting and fixing it in the closed cavity 23. In addition, the sample injection port 221, the visual window 222 and the opening 223 are respectively arranged on the upper cover 22, so that one end of the sleeve 41 is arranged around the opening 223 and fixed on the upper cover 22, which is conducive to the processing and manufacturing of the immunoassay reagent card and reduces the production cost.

[0052] Specifically, in another embodiment of the present application, please refer to Figure 5 The visual window 222 is a perspective sheet sealed and installed in the observation window of the upper cover 22. The perspective sheet can be a transparent glass sheet, a transparent film or a transparent film, so that the cavity 23 of the housing 2 forms a closed space environment. Among them, the transparent glass sheet, the transparent film or the transparent film can be integrally formed with the upper cover 22 to facilitate processing and improve sealing performance. Of course, the transparent glass sheet, the transparent film or the transparent film can also be sealed and combined with the observation window of the upper cover 22 by bonding.

[0053] In another embodiment of the present application, please refer to Figure 1 , Figure 4 and Figure 5 The reagent kit structure 4 also includes a piston structure 43 for pushing the reagent cup 42 toward the cavity 23. The piston structure 43 is slidably installed in the sleeve 41. The end of the reagent cup 42 facing away from the closed end 422 is an open end 421, and the end of the piston structure 43 is sealed on the open end 421 of the reagent cup 42.

[0054] In this embodiment, the reagent box structure 4 also includes a piston structure 43 for pushing the reagent cup 42 toward the direction of the cavity 23. When releasing the reagent onto the test strip 1 in the closed cavity 23 of the housing 2, it is only necessary to press the piston structure 43 in the sleeve 41 to push the reagent cup 42 to move and place it into the cavity 23 of the housing 2. The piercing member 5 in the cavity 23 can pierce the closed end 422 of the reagent cup 42, and the reagent encapsulated in the reagent cup 42 can be quickly released onto the test strip 1. The operation is simple and convenient, and the detection efficiency is improved. In addition, the end of the reagent cup 42 facing away from the closed end 422 is set as an open end 421, and the end of the piston structure 43 is sealed on the open end 421 of the reagent cup 42, so that the reagent cup 42 forms a closed space, which can prevent the volatile harmful substances in the reagent from polluting the environment and affecting human health. In addition, after the test is completed, due to the sealing cooperation between the piston structure 43 and the sleeve 41, the samples and reagents are sealed and stored in the closed cavity 23 of the shell 2, which reduces the difficulty of collecting and processing the subsequent test waste and avoids biological infection hazards to the operator during the collection, transportation and processing of the waste.

[0055] In another embodiment of the present application, please refer to Figure 4 and Fig.10 The piston structure 43 includes a piston body 431 movably mounted in the sleeve 41 and a push rod 432 connected to the piston body 431. The end of the piston body 431 is sealed on the open end 421 of the reagent cup 42, and one end of the push rod 432 away from the piston body 431 protrudes from the port at the other end of the sleeve 41.

[0056] In this embodiment, the piston structure 43 includes a piston body 431 and a push rod 432 connected to the piston body 431, and an end of the push rod 432 away from the piston body 431 is protruded from the port at the other end of the sleeve 41, so that the user can press the push rod 432 to quickly push the reagent cup 42 toward the closed cavity 23 of the shell 2, thereby facilitating the column to quickly puncture the closed end 422 of the reagent cup 42 and improve the detection efficiency.

[0057] In another embodiment of the present application, in order to prevent leakage or volatilization of the reagent in the reagent cup 42, after adding the reagent, the open end 421 of the reagent cup 42 is sealed with aluminum foil or plastic film, and the end of the piston body 431 is pressed against the open end 421 of the reagent cup 42 to enhance the sealing of the reagent cup 42 by the aluminum foil or plastic film, thereby preventing volatile harmful substances in the reagent from polluting the environment.

[0058] In another embodiment of the present application, please refer to Figure 4 and Fig.10 An annular groove 433 is formed at the connection between the push rod 432 and the piston body 431 , and a sealing ring 6 is installed in the annular groove 433 .

[0059] In this embodiment, an annular groove 433 is provided at the connection between the push rod 432 and the piston body 431, and a sealing ring 6 is installed in the annular groove 433 to enhance the sealing between the piston structure 43 and the inner wall of the sleeve 41, thereby preventing the leakage of reagents during transportation or detection, and further preventing the volatile harmful substances in the reagents from polluting the environment and affecting human health. In addition, after the detection is completed, the sample and the reagent are sealed and stored in the closed cavity 23 of the housing 2, which reduces the difficulty of collecting and processing the waste of subsequent detection, and avoids the biological infection hazard to the operator during the collection, transportation and processing of the waste.

[0060] In another embodiment of the present application, please refer to Figure 3 and Figure 5 The lower shell 21 is provided with a plurality of insertion rods 211 , and the upper cover 22 is provided with corresponding insertion holes 224 for the insertion of the insertion rods 211 .

[0061] In this embodiment, by providing a plurality of plug rods 211 on the shell, and correspondingly providing plug holes 224 for the plug rods 211 to be inserted on the upper cover 22, the upper cover 22 can be quickly connected to the lower shell 21 by only inserting the plug rods 211 into the corresponding plug holes 224, which is simple to operate and convenient to disassemble and assemble. Of course, the upper cover 22 and the lower shell 21 can also be connected by a snap-fit ​​structure known to those skilled in the art, and the specific structural form of the snap-fit ​​structure can be selected and set according to actual needs, which will not be described in detail here.

[0062] In another embodiment of the present application, please refer to Figure 5 A locking structure 7 for positioning the end of the test strip 1 away from the storage tank 9 is provided on the inner bottom surface of the lower shell 21 .

[0063] In this embodiment, a locking structure 7 is provided on the inner bottom surface of the lower shell 21, and the end of the test strip 1 away from the storage tank 9 is placed in the locking structure 7 to position the end of the test strip 1 away from the storage tank 9. This can improve the stability of the absorbent pad of the test strip 1, facilitate the smooth flow of reagents or samples with higher viscosity along the length direction of the test strip 1, and improve the chromatographic detection effect.

[0064] Specifically, in another embodiment of the present application, please refer to Figure 5 The clamping structure 7 includes two parallel and spaced clamping plates 71, and a clamping position 72 is formed between the two clamping plates 71 to accommodate and position the end of the test strip 1 away from the storage tank 9. Of course, the clamping structure 7 can also be a clamping slot directly opened on the fixed block fixed in the lower shell 21, and the end of the test strip 1 away from the storage tank 9 is placed in the clamping slot, which can play a positioning role for the end of the test strip 1 away from the storage tank 9. Furthermore, reinforcing ribs supporting the test strip 1 are also provided on the inner bottom surface of the lower shell 21 to enhance the stability of the absorbent pad of the test strip 1, which is conducive to the smooth flow of reagents or samples with higher viscosity along the length direction of the test strip 1, thereby improving the chromatographic detection effect.

[0065] In another embodiment of the present application, please refer to Figure 3 and Figure 7 A plurality of pressure columns 8 for supporting the test strip 1 are provided at intervals on the upper cover 22. The test strip 1 can be pressed and fixed in the lower shell 21 by the pressure columns 8. Since the contact area between the pressure columns 8 and the test strip 1 is small, the sample reagent or product with higher viscosity can flow more smoothly along the length direction of the test strip 1, thereby improving the detection efficiency.

[0066] In this embodiment, a plurality of pressure posts 8 for supporting the test strip 1 are provided at intervals on the upper cover 22. The test strip 1 is pressed and fixed in the lower shell 21 by the pressure posts 8. Since the contact area between each pressure post 8 and the test strip 1 is small, the reagent and sample are prevented from being laterally blocked and flowing in multiple directions, so that the reagent and sample can flow smoothly along the length direction of the test strip 1.

[0067] In another embodiment of the present application, please refer to Figure 3 and Figure 7 Each pressure column 8 is a cylindrical structure, and the diameter of each pressure column 8 is smaller than the width of the test strip 1.

[0068] In this embodiment, each pressing column 8 is set to a cylindrical structure to enhance the force of each pressing column 8 to press the test strip 1, so that the test strip 1 is firmly clamped between the upper cover 22 and the lower shell 21, and the test strip 1 is not easy to move. In addition, the diameter of each pressing column 8 is set to be smaller than the width of the test strip 1 to reduce the contact area between each pressing column 8 and the test strip 1, so as to avoid the phenomenon of multi-directional overflow caused by the reagent and the sample being squeezed and blocked in the longitudinal direction (parallel to the thickness direction of the test strip 1), thereby facilitating the smooth flow of the reagent and the sample along the length direction of the test strip 1 and improving the detection efficiency.

[0069] In another embodiment of the present application, please refer to Figure 3 and Figure 7 A plurality of pressure columns 8 are spaced apart along the length direction of the test strip 1 , the axial direction of each pressure column 8 is parallel to the thickness direction of the test strip 1 , and one end of each pressure column 8 away from the upper cover 22 is pressed against the test strip 1 .

[0070] In the present embodiment, a plurality of pressure columns 8 are distributed at intervals along the length direction of the test strip 1, and the axial direction of each pressure column 8 is parallel to the thickness direction of the test strip 1, and one end of each pressure column 8 away from the upper cover 22 is pressed tightly against the test strip 1, so that the test strip 1 is subjected to a balanced pressure force, which enhances the stability of the test strip 1 and facilitates the smooth flow of reagents and samples along the length direction of the test strip 1 to a certain extent, thereby facilitating the improvement of detection efficiency.

[0071] In another embodiment of the present application, please refer to Figure 3 and Figure 6 A storage tank 9 for accommodating reagents is provided at a position corresponding to the opening 223 in the cavity 23 , the opening 223 is connected to the storage tank 9 , and one end of the test strip 1 is placed in the storage tank 9 .

[0072] In this embodiment, by arranging a storage tank 9 in the cavity 23, and the position of the storage tank 9 corresponds to the position of the opening 223, after the closed end 422 of the reagent cup 42 is punctured, the reagent released from the reagent cup 42 can be stored in the storage tank 9, thereby preventing the reagent from overflowing in the cavity 23 of the housing 2. In addition, by placing one end of the test strip 1 in the storage tank 9, the reagent in the storage tank 9 can be evenly mixed with the sample in the form of lateral flow (flowing along the length direction of the test strip 1) through the test strip 1, which is conducive to improving the accuracy of the detection.

[0073] In another embodiment of the present application, please refer to Figure 6 A slot 91 for holding the test strip 1 is provided on the side wall of the storage tank 9 , and one end of the test strip 1 extends through the slot 91 into the storage tank 9 .

[0074] In this embodiment, a bayonet 91 is provided on the side wall of the storage tank 9, and the bayonet 91 is utilized to hold and position the portion of the test strip 1 placed in the storage tank 9, thereby enhancing the stability of the test strip 1, facilitating the smooth flow of reagents along the length direction of the test strip 1, and improving the detection efficiency and detection effect.

[0075] In another embodiment of the present application, please refer to Figure 6 A positioning groove 92 for accommodating and positioning the test strip 1 is provided at the bottom of the storage tank 9 .

[0076] In this embodiment, a positioning groove 92 is provided at the bottom of the storage tank 9, and one end of the test strip 1 is placed in the storage tank 9 to be accommodated in the positioning groove 92, so that the test strip 1 has a larger contact area with the reagent, thereby enhancing the stability of the test strip 1, facilitating the smooth flow of the reagent along the length direction of the test strip 1, and improving the detection efficiency and detection effect.

[0077] In another embodiment of the present application, please refer to Figure 4 The test strip 1 includes a liquid absorbing pad and a bottom plate supporting the liquid absorbing pad, and the bottom plate is supported on the inner bottom surface of the lower shell 21.

[0078] In this embodiment, the absorbent pad is supported in the lower shell 21 by the bottom plate, which can improve the stability of the absorbent pad and facilitate the smooth flow of samples with high viscosity along the length direction of the test strip 1. By providing a positioning groove 92 at the bottom of the storage tank 9 and accommodating the bottom plate in the positioning groove 92, the bottom plate can be prevented from contacting the reagent in the storage tank 9, and only the absorbent pad supported on the bottom plate is directly in contact with the reagent in the storage tank 9, so that the test strip 1 has a larger contact area with the reagent, which is conducive to the uniform mixing of the reagent and the sample, and improves the detection efficiency.

[0079] In another embodiment of the present application, please refer to Figure 4 and Figure 6 The piercing member 5 is a column with one end fixed in the cavity 23 , and the other end of the column extends into the opening 223 .

[0080] In this embodiment, by setting the puncture member 5 as a column, one end of the column is fixed in the cavity 23, and the other end of the column extends to the opening 223, when the piston structure 43 pushes the reagent cup 42 to move toward the cavity 23 to the opening 223, the column can quickly puncture the closed end 422 of the reagent cup 42, so that the reagent encapsulated in the reagent cup 42 is quickly released onto the test strip 1, which is simple and convenient to operate and improves the detection efficiency.

[0081] In another embodiment of the present application, please refer to Figure 6 The diameter of the column gradually decreases from the end away from the reagent cup 42 to the end close to the reagent cup 42.

[0082] In this embodiment, by gradually reducing the diameter of the column from the end away from the reagent cup 42 to the end close to the reagent cup 42, the end of the column close to the reagent cup 42 is pointed, reducing the contact area between the column and the reagent cup 42, which is conducive to the column quickly piercing the closed end 422 of the reagent cup 42, so that the reagent encapsulated in the reagent cup 42 is quickly released onto the test strip 1, which is simple and convenient to operate and improves the detection efficiency.

[0083] In another embodiment of the present application, please refer to Figure 4 and Figure 6 The column is located in the storage tank 9, and the axial direction of the column is parallel to the axial direction of the sleeve 41. While ensuring that the column can pierce the closed end 422 of the reagent cup 42, the column can also be used to stop the end of the test strip 1 placed in the storage tank 9, that is, the length of the test strip 1 extending into the storage tank 9 can be limited to achieve the purpose of rapid chromatographic detection, and the test strip 1 can be prevented from moving along its length direction, which can improve the stability of the test strip 1, facilitate the smooth flow of reagents in the storage tank 9 along the length direction of the test strip 1, and improve the chromatographic detection effect.

[0084] In another embodiment of the present application, please refer to Figure 4 and Fig. 9 The reagent kit structure 4 also includes a sliding structure for guiding the reagent cup 42 to move in the sleeve 41, the sliding structure includes a slider 423 and a slide groove 411 slidably matched with the slider 423, the slider 423 is convexly arranged on the outer wall of the reagent cup 42, and the slide groove 411 is concavely arranged on the inner wall of the sleeve 41.

[0085] In this embodiment, a sliding structure is provided to guide the reagent cup 42 to move in the sleeve 41, and the sliding structure includes a slider 423 and a slide groove 411 that slidably cooperates with the slider 423. The slider 423 is convexly provided on the outer wall of the reagent cup 42, and the slide groove 411 is concavely provided on the inner wall of the sleeve 41. In the process that the piston structure 43 pushes the reagent cup 42 to move toward the cavity 23 of the shell 2, under the action of the slide groove 411 and the sliding cooperation with the slider 423, not only the stability and reliability of the movement of the reagent cup 42 can be enhanced, but also the friction between the outer wall of the reagent cup 42 and the inner wall of the sleeve 41 can be reduced, so as to facilitate the rapid movement of the reagent cup 42, thereby facilitating the rapid puncture of the closed end 422 of the reagent cup 42 by the column, thereby improving the detection efficiency.

[0086] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. An immunoassay reagent card, characterized in that: include: A shell body, provided with a sealed cavity and a sample adding port communicated with the cavity; A test strip is placed and fixed in the cavity; A sealing plug, used for sealing the sample addition port; and A reagent kit structure, used for pre-sealing and packaging reagents, the reagent kit structure comprising a sleeve with one end fixed to the housing and a reagent cup slidably mounted in the sleeve; The shell is provided with a visual window for observing the test status of the test strip, and the shell is also provided with an opening for the closed end of the reagent cup to pass through, the opening is connected to the cavity, and a piercing member for piercing the closed end of the reagent cup is correspondingly provided in the cavity; A storage tank for accommodating reagents is provided at a position in the cavity corresponding to the opening, the opening is connected to the storage tank, and one end of the test strip is placed in the storage tank; The piercing member is a column with one end fixed in the cavity, and the other end of the column extends into the opening.

2. The immunoassay reagent card according to claim 1, characterized in that: A snap-in opening for holding the test strip is provided on the side wall of the storage tank, and one end of the test strip passes through the snap-in opening and extends into the storage tank.

3. The immunoassay reagent card according to claim 2, characterized in that: The bottom of the storage tank is provided with a positioning groove for accommodating and positioning the test strip.

4. The immunoassay reagent card according to claim 1, characterized in that: The diameter of the column gradually decreases from an end away from the reagent cup to an end close to the reagent cup.

5. The immunoassay reagent card according to claim 1, characterized in that: The reagent kit structure also includes a sliding structure for guiding the reagent cup to move in the sleeve, the sliding structure includes a slider and a slide groove slidably matched with the slider, the slider is convexly arranged on the outer wall of the reagent cup, and the slide groove is concavely arranged on the inner wall of the sleeve.

6. The immunoassay reagent card according to claim 1, characterized in that: The shell body comprises a lower shell and an upper cover detachably connected to the lower shell, the sample loading port, the visual window and the opening are respectively arranged on the upper cover, one end of the sleeve is arranged around the opening and fixed on the upper cover, and the upper cover is sealed and connected to the lower shell to form the closed cavity.

7. The immunoassay reagent card according to any one of claims 1 to 6, characterized in that: The reagent kit structure also includes a piston structure for pushing the reagent cup toward the cavity, the piston structure is slidably installed in the sleeve, the end of the reagent cup facing away from the closed end is an open end, and the end of the piston structure is sealed on the open end of the reagent cup.

8. The immunoassay reagent card according to claim 7, characterized in that: The piston structure includes a piston body and a push rod connected to the piston body, the end of the piston body away from the push rod is sealed on the open end of the reagent cup, and the end of the push rod away from the piston body protrudes from the port at the other end of the sleeve.

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