A centrifugal microfluidic chip

Abstract

The present application relates to a centrifugal microfluidic chip. Specifically, the present application provides a centrifugal microfluidic chip, characterized in that the centrifugal microfluidic chip comprises a channel disc; the channel disc is provided with a plurality of liquid storage cavities, a circular reagent cavity is arranged in the central region of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity, each liquid storage cavity is connected with a main microchannel, the distal end of the main microchannel away from the liquid storage cavity is communicated with a first exhaust cavity through a main hole, the proximal end of the main microchannel close to the main hole is provided with a left microchannel and a right microchannel, and the distal ends of the left microchannel and the right microchannel are communicated with a second exhaust cavity and a third exhaust cavity through a left hole and a right hole respectively; the channel disc is provided with a liquid inlet, the liquid inlet is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with exhaust holes. The centrifugal microfluidic chip can realize one-time filling of multi-channel gel, thereby improving the efficiency of gel electrophoresis experiment.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic chip, in particular, the present application relates to a centrifugal microfluidic chip. BACKGROUND

[0002] In the prior art, gel electrophoresis experiment is completely completed by manual operation experiment, the main steps are installation of electrophoresis tank, preparation of gel, pouring of gel into electrophoresis tank, after the gel poured into the electrophoresis tank is solidified, manual addition of S buffer, sample and other steps are still needed, especially in the preparation process of gel electrophoresis experiment gel, the preparation efficiency of gel channel in the prior art is very low, for the gel electrophoresis experiment which needs a large number of gel channels, the preparation of gel channel in the prior art consumes a lot of manpower, material resources and time cost, which greatly reduces the efficiency of gel electrophoresis experiment, therefore, how to improve the efficiency in the process of gel electrophoresis experiment becomes a difficulty.

[0003] Therefore, there is a need in the art to develop a device for improving the preparation efficiency of gel channel and improving the efficiency of gel electrophoresis experiment. SUMMARY

[0004] The first purpose of the present application is to improve the preparation efficiency of gel channel and the efficiency of gel electrophoresis experiment.

[0005] Another purpose of the present application is to provide a clamping device of centrifugal microfluidic chip which is convenient to install, increases the length and volume of the channel of centrifugal microfluidic chip and ensures that the centrifugal microfluidic chip will not fall off in the centrifugal process.

[0006] The first aspect of the present application provides a centrifugal microfluidic chip, the centrifugal microfluidic chip comprises a channel disc 2.1;

[0007] The channel disc is provided with a plurality of liquid storage cavities 2.2, the center area of the channel disc is provided with a circular reagent cavity, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main microchannel 2.4, the end of the main microchannel away from the liquid storage cavity is communicated with a first exhaust cavity through a main hole 2.5, the end of the main microchannel close to the main hole is provided with a left microchannel 2.6 and a right microchannel 2.7, the ends of the left microchannel and the right microchannel are communicated with a second exhaust cavity and a third exhaust cavity through a left hole 2.8 and a right hole 2.9 respectively;

[0008] The channel disc is provided with a liquid inlet 2.10, the liquid inlet is communicated with the liquid storage cavity or the circular reagent cavity, the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with exhaust holes 2.11.

[0009] In another preferred embodiment, the liquid storage chamber, the circular reagent chamber, the main microchannel, the left microchannel, the right microchannel, the main hole, the left hole and / or the right hole are electrically conductive.

[0010] In another preferred embodiment, each of the liquid storage chambers is independent of each other.

[0011] In another preferred embodiment, the channel disc is a circular disc.

[0012] In another preferred embodiment, the end of the left microchannel and the right microchannel refers to the end away from the main microchannel.

[0013] In another preferred embodiment, the liquid inlet is provided with a glue film 2.18.

[0014] In another preferred embodiment, the number of liquid inlets is one or more.

[0015] In another preferred embodiment, the number of liquid inlets is 2-6, preferably 2-3.

[0016] In another preferred embodiment, the first exhaust chamber includes a first exhaust channel 2.12 and a first excess reagent chamber 2.13.

[0017] In another preferred embodiment, the second exhaust chamber includes a second exhaust channel 2.14 and a second excess reagent chamber 15.

[0018] In another preferred embodiment, the third exhaust chamber includes a third exhaust channel 2.16 and a third excess reagent chamber 2.17.

[0019] In another preferred embodiment, the exhaust hole is provided on the first excess reagent chamber.

[0020] In another preferred embodiment, the exhaust hole is provided on the second excess reagent chamber.

[0021] In another preferred embodiment, the exhaust hole is provided on the third excess reagent chamber.

[0022] In another preferred embodiment, the main hole is communicated with the first excess reagent chamber through the first exhaust channel.

[0023] In another preferred embodiment, the left hole is communicated with the second excess reagent chamber through the second exhaust channel.

[0024] In another preferred embodiment, the right hole is communicated with the third excess reagent chamber through the third exhaust channel.

[0025] In another preferred embodiment, the liquid storage chamber, the circular reagent chamber, the main microchannel, the left microchannel and / or the right microchannel are arranged near the lower surface side of the channel disc.

[0026] In another preferred embodiment, the first exhaust chamber, the second exhaust chamber and / or the third exhaust chamber is disposed near the upper surface side of the channel disc.

[0027] In another preferred embodiment, the sum of the volumes of the circular reagent chamber and each reservoir chamber is greater than the sum of the volumes of each main microchannel, each left microchannel, each right microchannel, each main orifice, each main orifice and each main orifice.

[0028] In another preferred embodiment, the hydrophilicity of the main microchannel, the left microchannel and the right microchannel is the same.

[0029] In another preferred embodiment, the number of reservoir chambers is 2-60, preferably 6-40, more preferably 8-30, and most preferably 10-30.

[0030] In another preferred embodiment, the hydrophilicity of the main microchannel is less than the hydrophilicity of the reservoir chamber.

[0031] In another preferred embodiment, the hydrophilicity of each main microchannel is less than the hydrophilicity of the reservoir chamber connected thereto.

[0032] In another preferred embodiment, the reservoir chamber is a hydrophilic material or the surface thereof is subjected to hydrophilic treatment.

[0033] In another preferred embodiment, the main microchannel is a hydrophobic material or the surface thereof is subjected to hydrophobic treatment.

[0034] In another preferred embodiment, each reservoir chamber is uniformly arranged around the circular reagent chamber; and / or

[0035] The center of the channel disc coincides with the center of the circular reagent chamber.

[0036] In another preferred embodiment, the main microchannel is distributed along the center of the channel disc toward the outer periphery of the channel disc.

[0037] In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm 2 ≤ S1 ≤ 0.005 mm 2 ; and / or

[0038] The area S2 of the longitudinal section of the left microchannel is 0.003 mm 2 ≤ S2 ≤ 0.007 mm 2 ; and / or

[0039] The area S3 of the longitudinal section of the right microchannel is 0.003 mm 2 ≤ S3 ≤ 0.007 mm 2 .

[0040] In another preferred embodiment, the longitudinal section of the left microchannel is rectangular, with a length of 0.10-0.14 mm and a width of 0.03-0.05 mm.

[0041] In another preferred embodiment, the longitudinal section of the main microchannel is rectangular, with a length of 0.06-0.10 mm and a width of 0.03-0.05 mm.

[0042] In another preferred embodiment, the longitudinal section of the right microchannel is rectangular, with a length of 0.10-0.14 mm and a width of 0.03-0.05 mm.

[0043] In another preferred embodiment, the main microchannel, the left microchannel and / or the right microchannel is a straight channel.

[0044] In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and / or the right microchannel is circular, rectangular or square.

[0045] In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and / or the right microchannel is rectangular.

[0046] In another preferred embodiment, the main microchannel, the left microchannel and / or the right microchannel is a straight channel.

[0047] In another preferred embodiment, the longitudinal sections of the left microchannel and the right microchannel are equal in area.

[0048] In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8-2 times, preferably 1.0-2.0 times, more preferably 1.2-1.8 times, more preferably 1.3-1.7 times, and most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.

[0049] In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8-2 times, preferably 1.0-2.0 times, more preferably 1.2-1.8 times, more preferably 1.3-1.7 times, and most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.

[0050] In another preferred embodiment, the angle a1 between the left microchannel and the main microchannel is 56±0.5°; and / or

[0051] the angle a2 between the right microchannel and the main microchannel is 56±0.5°.

[0052] In another preferred embodiment, the angle a1 between the left microchannel and the main microchannel is equal to the angle a2 between the right microchannel and the main microchannel.

[0053] In another preferred embodiment, each reservoir is a long-hole reservoir with a size tolerance of 0.015-0.025 mm and a depth of 4.8-5.2 mm.

[0054] In another preferred embodiment, the centrifugal microfluidic chip is made of transparent material.

[0055] In another preferred embodiment, the circular reagent reservoir is raised on the channel disc.

[0056] In another preferred embodiment, the channel disc is further provided with positioning hole 2.19.

[0057] In a second aspect of the present application, a centrifugal microfluidic chip clamping device is provided, which comprises a centrifugal microfluidic chip positioning part and a transmission part.

[0058] The centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal pressure hand shaft 1.2 and a centrifugal pressure hand 1.3, wherein the centrifugal pressure hand is provided with a through hole 1.4.

[0059] The centrifugal microfluidic chip positioning tray is provided with a suspension through hole 1.5 at the periphery, the centrifugal pressure hand shaft passes through the through hole of the centrifugal pressure hand, and the two ends of the centrifugal pressure hand shaft pass through the suspension through hole, and the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft.

[0060] In the non-centrifugal state, the centrifugal pressure hand is in a suspended state, and in the centrifugal state, the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft with the lower end outward and upward and the upper end inward and downward.

[0061] The centrifugal microfluidic chip positioning tray is connected to the transmission part, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate.

[0062] In another preferred embodiment, the chip centrifugal pressure hand shaft is a cylindrical shaft.

[0063] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is a disc.

[0064] In another preferred embodiment, the number of suspension through holes is 2-8, more preferably 3-6.

[0065] In another preferred embodiment, the cross-sectional area of the through hole is greater than or equal to the cross-sectional area of the centrifugal pressure hand shaft.

[0066] In another preferred embodiment, in the non-centrifugal state, there is a gap between the through hole of the centrifugal pressure hand and the centrifugal pressure hand shaft.

[0067] In another preferred embodiment, the gap has a height of 1-4 mm in the non-centrifugal state.

[0068] In another preferred embodiment, the center of gravity of the centrifugal pressure hand is located below the perforation in the non-centrifugal state.

[0069] In another preferred embodiment, the perforation is a waist-shaped through hole.

[0070] In another preferred embodiment, the centrifugal pressure hand is a "Z"-shaped centrifugal pressure hand, which includes a pressure hand head 1.6, a pressure hand body 1.7, and a pressure hand tail 1.8, the pressure hand head forms an angle of 50-110° with the pressure hand body, the pressure hand tail forms an angle of 70-110° with the pressure hand body, and the angle formed by the pressure hand head and the pressure hand body is in the opposite direction of the angle formed by the pressure hand tail and the pressure hand body.

[0071] In another preferred embodiment, the angle formed by the pressure hand head and the pressure hand body is directed towards the microfluidic chip positioning tray.

[0072] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a pressing hole 1.9 for pressing the centrifugal pressure hand shaft.

[0073] In another preferred embodiment, the pressing hole is a pressing threaded hole.

[0074] In another preferred embodiment, a screw is screwed on the pressing threaded hole for pressing the chip centrifugal pressure hand shaft.

[0075] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a mounting groove, and the centrifugal pressure hand shaft is arranged on the mounting groove.

[0076] In another preferred embodiment, the transmission part includes a motor 1.10 and a rotating shaft 1.11.

[0077] In another preferred embodiment, the rotating shaft is connected to the motor, and the microfluidic chip positioning tray is connected to the rotating shaft.

[0078] In another preferred embodiment, the motor is provided with a motor shaft, and the rotating shaft is connected to the motor shaft of the motor through a diaphragm coupling.

[0079] In another preferred embodiment, the motor is a servo motor.

[0080] In another preferred embodiment, the bottom center of the centrifugal microfluidic chip positioning tray is connected to the transmission part.

[0081] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with a positioning pin 1.12.

[0082] In another preferred embodiment, the number of positioning pins is 2-6.

[0083] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with groove positions for placing microfluidic chips on the upper surface of the tray.

[0084] In another preferred embodiment, the groove positions are circular groove positions.

[0085] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is provided with a plurality of long strip-shaped holes.

[0086] In a third aspect of the present application, a gel electrophoresis device is provided, which comprises the centrifugal microfluidic chip according to the first aspect of the present application and the centrifugal microfluidic chip clamping device according to the second aspect of the present application.

[0087] In another preferred embodiment, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.

[0088] It should be understood that, within the scope of the present application, the above-mentioned technical features of the present application and the technical features specifically described in the following (such as the examples) can be combined with each other to form new or preferred technical solutions. Due to the limited space, they will not be listed one by one here. BRIEF DESCRIPTION OF DRAWINGS

[0089] The drawings are only used for illustrative description and should not be understood as limiting the patent; in order to better illustrate the embodiments, some components in the drawings may be omitted, enlarged or reduced, and do not represent the actual product size; it is understandable for those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings; the same or similar reference numerals correspond to the same or similar components; the positional relationship described in the drawings is only used for illustrative description and should not be understood as limiting the patent.

[0090] Figure 1 It is a schematic diagram of the whole channel disc of the centrifugal microfluidic chip.

[0091] Figure 2 It is a schematic diagram of the cross-section structure of the channel disc, in which A is the upper cross-section, B is the middle cross-section, and C is the lower cross-section.

[0092] Figure 3 It is a schematic diagram of the lower surface structure of the middle cross-section of the channel disc.

[0093] Figure 4 It is a schematic diagram of the D area of the lower surface of the middle cross-section of the channel disc.

[0094] Figure 5 It is a schematic diagram of the long hole type liquid storage cavity structure.

[0095] Figure 6 The structure diagram of the centrifugal micro-fluidic chip positioning tray.

[0096] Figure 7 The structure diagram of the centrifugal pressure hand.

[0097] Figure 8 The overall structure diagram of the centrifugal micro-fluidic chip clamping device with the centrifugal micro-fluidic chip installed.

[0098] Figure 9 The side sectional view of the centrifugal micro-fluidic chip clamping device with the centrifugal micro-fluidic chip installed.

[0099] Figure 10 The state of the centrifugal pressure hand in the non-centrifugal state.

[0100] Figure 11 The state of the centrifugal pressure hand in the centrifugal state.

[0101] In the drawings, the numbers represent:

[0102] 1.1 is the centrifugal micro-fluidic chip positioning tray, 1.2 is the centrifugal pressure hand shaft, 1.3 is the centrifugal pressure hand, 1.4 is the through hole, 1.5 is the suspension through hole, 1.6 is the pressure hand head, 1.7 is the pressure hand body, 1.8 is the pressure hand tail, 1.9 is the pressure hole, 1.10 is the motor, 1.11 is the rotating shaft, and 1.12 is the positioning pin;

[0103] 2.1 is the channel disc, 2.2 is the liquid storage cavity, 2.3 is the circular reagent cavity, 2.4 is the main micro-channel, 2.5 is the main hole, 2.6 is the left micro-channel, 2.7 is the right micro-channel, 2.8 is the left hole, 2.9 is the right hole, 2.10 is the liquid inlet, 2.11 is the exhaust hole, 2.12 is the first exhaust channel, 2.13 is the first excess reagent cavity, 2.14 is the second exhaust channel, 2.15 is the second excess reagent cavity, 2.16 is the third exhaust channel, 2.17 is the third excess reagent cavity, 2.18 is the adhesive film, and 2.19 is the positioning hole; DETAILED DESCRIPTION

[0104] The inventor has developed a centrifugal micro-fluidic chip clamping device and a centrifugal micro-fluidic chip for the first time through extensive and in-depth research.

[0105] In the centrifugal microfluidic chip, the microfluidic chip comprises a channel disc, a proper amount of gel is filled into the centrifugal microfluidic chip through the feeding port of the channel disc, the centrifugal microfluidic chip is placed in an existing centrifugal device, the gel is filled into the corresponding main microchannel, left microchannel and right microchannel through centrifugation, and the filling of the gel is completed when the gel appears in the first exhaust cavity, the second exhaust cavity and the third exhaust cavity.

[0106] In the centrifugal microfluidic chip clamping device, the outer periphery of the centrifugal microfluidic chip positioning tray is provided with a hanging through hole, the centrifugal pressure hand shaft penetrates the through hole of the centrifugal pressure hand, and the two ends of the centrifugal pressure hand shaft penetrate the hanging through hole, the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft, in the non-centrifugal state, the centrifugal pressure hand is in a hanging state, and in the centrifugal state, the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft, with the lower end rotating outward and upward, and the upper end rotating inward and downward. The greater the centrifugal force of the centrifugal microfluidic chip clamping device, the greater the clamping force of the centrifugal pressure hand on the microfluidic chip, so that the centrifugal microfluidic chip cannot fall off during centrifugation.

[0107] On this basis, the inventor has completed the present application.

[0108] Terms

[0109] As used herein, the terms "comprising", "including", "containing", "have" and "including" are used interchangeably and include not only the closed definition but also the semi-closed and open definition. In other words, the terms include "consisting of", "consisting essentially of".

[0110] As used herein, the terms "up", "down", "horizontal", "left", "right", etc. indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0111] As used herein, the term "connection" should be understood broadly, which can be fixed connection, detachable connection or integral connection.

[0112] As used herein, the term "a plurality of" means 2 or more.

[0113] As used herein, the term "non-centrifugal state" is also referred to as horizontal static state, which refers to the state that the centrifugal microfluidic chip clamping device is statically located on the horizontal plane, and the microfluidic chip positioning tray is not rotated.

[0114] As used herein, the term "centrifugal state" is also referred to as working state, which refers to the state that the transmission part drives the microfluidic chip positioning tray to rotate.

[0115] Centrifugal microfluidic chip

[0116] In order to overcome the shortcomings that gel needs to be manually poured into the electrophoresis tank one by one and only one electrophoresis tank can be filled at a time in the existing gel electrophoresis experiment, the present application develops a centrifugal microfluidic chip capable of automatically filling multiple gel channels at one time by centrifugation.

[0117] The centrifugal microfluidic chip of the present application will be described below in conjunction with the accompanying drawings Figures 1-5 It should be understood that the accompanying drawings are only used for illustrative description and cannot be understood as a limitation of the present patent.

[0118] Typically, a centrifugal microfluidic chip comprises a channel disc 2.1;

[0119] The channel disc is provided with a plurality of liquid storage cavities 2.2, and a circular reagent cavity is arranged in the central region of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity 2.3, each liquid storage cavity is connected with a main microchannel 2.4, the end of the main microchannel away from the liquid storage cavity is communicated with a first exhaust cavity through a main hole 2.5, and the end of the main microchannel close to the main hole is provided with a left microchannel 2.6 and a right microchannel 2.7, and the ends of the left microchannel and the right microchannel are communicated with a second exhaust cavity and a third exhaust cavity through a left hole 2.8 and a right hole 2.9 respectively.

[0120] The channel disc is provided with a liquid adding port 2.10, the liquid adding port is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with exhaust holes 2.11.

[0121] In a preferred embodiment, the channel disc is a circular disc.

[0122] In another preferred embodiment, the number of liquid adding ports is one or more, preferably 2-6, and more preferably 2-3.

[0123] When there are multiple liquid adding ports, gel can be added to the liquid storage cavity and / or the circular reagent cavity from one of the liquid adding ports, and when the gel flows out from another liquid adding port, it indicates that the liquid storage cavity and / or the circular reagent cavity is full of gel.

[0124] In another preferred embodiment, the liquid inlet is provided with a glue film 18. The glue film can avoid the pollution of the cavity and the channel of the channel disc by the outside world.

[0125] In the centrifugal microfluidic chip, the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with exhaust holes, which can smoothly exhaust the air in the main microchannel, the left microchannel and the right microchannel during the gel filling process, and can avoid the gel filling in the main microchannel, the left microchannel and the right microchannel from being contaminated by air bubbles, thereby ensuring the quality of the gel filling.

[0126] In the present application, the positions of the liquid storage cavity, the circular reagent cavity, the main microchannel, the left microchannel, the right microchannel, the main hole channel, the left hole channel, the right hole channel, the first exhaust cavity, the second exhaust cavity and / or the third exhaust cavity in the channel disc are not particularly limited, as long as the purpose of the present application is met.

[0127] In a preferred embodiment of the present application, the liquid storage cavity, the circular reagent cavity, the main microchannel, the left microchannel and / or the right microchannel are arranged close to the lower surface side of the channel disc.

[0128] In another preferred embodiment, the first exhaust cavity, the second exhaust cavity and / or the third exhaust cavity are arranged close to the upper surface side of the channel disc.

[0129] In the present application, the number of the liquid storage cavities is not particularly limited and can be designed according to the purpose of the experiment. Preferably, the number of the liquid storage cavities is 2-60, preferably 6-40, more preferably 8-30, and most preferably 10-30.

[0130] In a preferred embodiment of the present application, the hydrophilicity of the main microchannel is smaller than that of the liquid storage cavity. Typically, the hydrophilicity of each main microchannel is smaller than that of the liquid storage cavity connected thereto. The hydrophilicity of the main microchannel being smaller than that of the liquid storage cavity ensures that the gel can simultaneously fill the main microchannel, the left microchannel and the right microchannel during centrifugation, and the gel simultaneously enters the first exhaust cavity, the second exhaust cavity and the third exhaust cavity from the main hole channel, the left hole channel and the right hole channel. Experimental research has found that if the hydrophilicity of part of the main microchannels is equal to or greater than that of the liquid storage cavity connected thereto, it cannot be ensured that the gel can simultaneously fill all the main microchannels, the left microchannels and the right microchannels during centrifugation. These part of the main microchannels will enter the first exhaust cavity, the second exhaust cavity and the third exhaust cavity in advance before or during centrifugation, and even pollute the instrument by being exhausted from the exhaust holes in advance, resulting in the failure of the gel filling,

[0131] In another preferred embodiment, the liquid storage cavity is a hydrophilic material or the surface thereof is subjected to hydrophilic treatment. In another preferred embodiment, the main microchannel is a hydrophobic material or the surface thereof is subjected to hydrophobic treatment.

[0132] In a preferred embodiment of the present application, the individual reservoirs are arranged uniformly around the circular reagent chamber. In another preferred embodiment, the center (center of the circle) of the channel disc coincides with the center (center of the circle) of the circular reagent chamber. In the present application, it is to be understood that the center (center of the circle) coincides also means that the channel disc and the circular reagent chamber form a concentric circle.

[0133] In another preferred embodiment, the main microchannel, the left microchannel and / or the right microchannel is a straight channel.

[0134] In another preferred embodiment, the longitudinal section of the main microchannel, the left microchannel and / or the right microchannel is circular, rectangular or square.

[0135] In a preferred embodiment of the present application, the main microchannel is distributed (extends) along the center of the channel disc to the outer periphery of the channel disc.

[0136] In another preferred embodiment, the area S1 of the longitudinal section of the main microchannel is 0.002 mm 2 ≤ S1 ≤ 0.005 mm 2 .

[0137] In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.003 mm 2 ≤ S2 ≤ 0.007 mm 2 .

[0138] In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.003 mm 2 ≤ S3 ≤ 0.007 mm 2 .

[0139] In another preferred embodiment, the longitudinal section of the left microchannel is rectangular, with a length of 0.10-0.14 mm and a width of 0.03-0.05 mm.

[0140] In another preferred embodiment, the longitudinal section of the main microchannel is rectangular, with a length of 0.06-0.10 mm and a width of 0.03-0.05 mm.

[0141] In another preferred embodiment, the longitudinal section of the right microchannel is rectangular, with a length of 0.10-0.14 mm and a width of 0.03-0.05 mm.

[0142] In another preferred embodiment, the area S2 of the longitudinal section of the left microchannel is 0.8-2 times, preferably 1.0-2 times, more preferably 1.2-1.8 times, more preferably 1.3-1.5 times, most preferably 1.4-1.6 times the area S1 of the section of the main microchannel.

[0143] In another preferred embodiment, the area S3 of the longitudinal section of the right microchannel is 0.8-2 times, preferably 1.0-2 times, more preferably 1.2-1.8 times, more preferably 1.3-1.5 times, and most preferably 1.4-1.6 times the area S1 of the longitudinal section of the main microchannel.

[0144] Preferably, the angle a1 between the left microchannel and the main microchannel is equal to the angle a2 between the right microchannel and the main microchannel.

[0145] The inventors have found through extensive experimental research that when the angle a1 between the left microchannel and the main microchannel and the angle a2 between the right microchannel and the main microchannel are not within the range of 56±0.5°, some of the first, second, and third vent cavities fail to discharge gel during centrifugal gel filling, while the remaining first, second, and third vent cavities do not contain gel, resulting in the main microchannel, the left microchannel, and the right microchannel being incompletely filled with gel, thus failing to meet the requirement of filling all the main microchannels, left microchannels, and right microchannels with gel.

[0146] In another preferred embodiment, each reservoir cavity is a long-hole type reservoir cavity, and the size tolerance of the long-hole type reservoir cavity is 0.015-0.025 mm, and the depth is 4.8-5.2 mm. A typical structure of a long-hole type reservoir cavity is shown in Figure 5 .

[0147] The gel in the long-hole type reservoir cavity is concentrated at the force point, i.e., the main microchannel opening, which can receive a greater centrifugal force, and can more effectively cause the gel to enter the main microchannel during centrifugation.

[0148] In another preferred embodiment, the circular reagent cavity is on the channel disc protrusion.

[0149] In another preferred embodiment, the channel disc is further provided with a positioning hole 2.19. The positioning hole can be combined with the positioning pin on the centrifugal microfluidic chip clamping device to prevent confusion of the positional relationship of each channel of the centrifugal microfluidic chip.

[0150] Centrifugal microfluidic chip preparation method

[0151] The preparation method of the centrifugal microfluidic chip is not particularly limited and can be prepared by using the methods commonly used in the prior art, such as mechanical cutting, film lamination, etc. For example, an upper film, a middle piece (the middle piece is provided with a plurality of cavity grooves and channel grooves, etc.), and a lower film are prepared, the upper film and the lower film are laminated with the middle piece, and the liquid inlet and the vent hole are punched to prepare the centrifugal microfluidic chip.

[0152] Centrifugal microfluidic chip clamping device

[0153] In order to overcome the drawbacks of the existing centrifugal microfluidic chip assembly device, the application provides a centrifugal microfluidic chip clamping device.

[0154] The centrifugal microfluidic chip clamping device of the application will be described below in conjunction with the accompanying drawings Figures 6-11 It should be understood that the accompanying drawings are only used for illustrative description and cannot be understood as a limitation of the patent.

[0155] Typically, the microfluidic chip clamping device comprises a centrifugal microfluidic chip positioning part and a transmission part.

[0156] The centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal pressure hand shaft 1.2 and a centrifugal pressure hand 1.3, wherein the centrifugal pressure hand is provided with a through hole 1.4.

[0157] The centrifugal microfluidic chip positioning tray is provided with a hanging through hole 1.5 on the outer periphery, the centrifugal pressure hand shaft passes through the through hole of the centrifugal pressure hand, and the two ends of the centrifugal pressure hand shaft pass through the hanging through hole, and the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft.

[0158] In the non-centrifugal state, the centrifugal pressure hand is in a hanging state, and in the centrifugal state, the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft with the lower end outward and upward and the upper end inward and downward.

[0159] The centrifugal microfluidic chip positioning tray is connected with the transmission part, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate.

[0160] In the application, it should be understood that the rotation of the centrifugal pressure hand around the centrifugal pressure hand shaft with the lower end outward and upward and the upper end inward and downward means that the upper end of the centrifugal pressure hand rotates towards the centrifugal microfluidic chip positioning tray, and the lower end rotates away from the centrifugal microfluidic chip positioning tray.

[0161] In the centrifugal microfluidic chip clamping device, the number of the hanging through holes is preferably multiple, for example, 2-8 or 3-6.

[0162] In a preferred embodiment of the application, in the non-centrifugal state, the center of gravity of the centrifugal pressure hand is located below the through hole.

[0163] In another preferred embodiment, the cross-sectional area of the perforation of the centrifugal pressure hand is greater than or equal to the cross-sectional area of the shaft of the centrifugal pressure hand. More preferably, in the un-centrifuged state, there is a gap between the perforation of the centrifugal pressure hand and the shaft of the centrifugal pressure hand. Typically, in the un-centrifuged state, the height of the gap is 1-4 mm.

[0164] In another preferred embodiment of the present application, the centrifugal pressure hand is a "Z" type centrifugal pressure hand, which comprises a pressure hand head, a pressure hand body and a pressure hand tail, the pressure hand head forms an angle of 50-110° with the pressure hand body, the pressure hand tail forms an angle of 70-110° with the pressure hand body, and the angle formed by the pressure hand head and the pressure hand body is in the opposite direction to the angle formed by the pressure hand tail and the pressure hand body.

[0165] In another preferred embodiment, the angle formed by the pressure hand head and the pressure hand body is directed towards the microfluidic chip positioning tray.

[0166] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a pressure tightening hole for tightening the shaft of the centrifugal pressure hand.

[0167] In another preferred embodiment, the pressure tightening hole is a pressure tightening threaded hole.

[0168] In another preferred embodiment, a screw is screwed onto the pressure tightening threaded hole for tightening the shaft of the chip centrifugal pressure hand.

[0169] In another preferred embodiment of the present application, the centrifugal microfluidic chip positioning tray is provided with positioning pins. The positioning pins are used for positioning the centrifugal microfluidic chip and preventing confusion. The number of positioning pins is preferably 2-6.

[0170] In another preferred embodiment of the present application, the centrifugal microfluidic chip positioning tray is provided with a plurality of long strip-shaped holes. The detection equipment can detect the centrifugal microfluidic chip through the long strip-shaped holes of the centrifugal microfluidic chip positioning tray.

[0171] In another preferred embodiment of the present application, the transmission part comprises a motor and a rotating shaft. Typically, the rotating shaft is connected to the motor, and the centrifugal microfluidic chip positioning tray is connected to the rotating shaft.

[0172] In another preferred embodiment, the shaft of the chip centrifugal pressure hand is cylindrical.

[0173] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is disc-shaped.

[0174] In another preferred embodiment, the centrifugal microfluidic chip positioning tray is further provided with a mounting groove, and the shaft of the centrifugal pressure hand is arranged on the mounting groove.

[0175] Gel electrophoresis device

[0176] The application also provides a gel electrophoresis device, which comprises the centrifugal microfluidic chip clamping device and the centrifugal microfluidic chip.

[0177] In another preferred embodiment, the centrifugal microfluidic chip is mounted on the centrifugal microfluidic chip positioning tray.

[0178] In the gel electrophoresis device, the centrifugal microfluidic chip clamping device and the centrifugal microfluidic chip are as described above.

[0179] The main advantages of the application include:

[0180] 1. In the centrifugal microfluidic chip of the application, a suitable amount of gel is manually filled into the centrifugal microfluidic chip through the filling port of the channel disc by using a pipette, and then the centrifugal microfluidic chip is placed in an existing centrifugal device, and the gel is filled into the corresponding main microchannel, left microchannel and right microchannel by centrifugation. When the first exhaust cavity, the second exhaust cavity and the third exhaust cavity appear, the gel filling is completed. Through a series of automatic operations, the gel electrophoresis experiment is finally completed. Therefore, the centrifugal microfluidic chip of the application can quickly and accurately realize the filling of multiple channel gels and improve the experimental (electrophoresis) efficiency.

[0181] 2. When the centrifugal microfluidic chip of the application fills the gel by centrifugation, the air in the main microchannel, the left microchannel and the right microchannel is discharged through the exhaust hole of the exhaust cavity under the action of centrifugation, which can avoid the gel filling in the main microchannel, the left microchannel and the right microchannel from being contaminated by air bubbles, and ensure the quality of gel filling.

[0182] 3. The centrifugal microfluidic chip of the application does not need an additional rotating shaft mounting hole (through hole) in the center position for centrifugal use, so that compared with the existing microfluidic chip hole with a rotating shaft mounting hole in the center, for the same size of centrifugal microfluidic chip, the centrifugal microfluidic chip of the application has a longer channel and a wider range of adaptation, that is, the diameter of the microfluidic chip can be greatly reduced, and there is a sufficient length of separation channel in DNA and protein gel electrophoresis.

[0183] 4. The centrifugal microfluidic chip clamping device of the application clamps the centrifugal microfluidic chip by centrifugal clamping. The greater the centrifugal force of the centrifugal microfluidic chip clamping device, the greater the clamping force of the centrifugal microfluidic chip clamping device on the centrifugal microfluidic chip, which can ensure that the centrifugal microfluidic chip does not fall off during centrifugation.

[0184] 5. The centrifugal microfluidic chip clamping device, in which the centrifugal pressure hand is in a suspended state in the non-centrifugal state, so that the centrifugal microfluidic chip can be conveniently placed and taken out, and the phenomenon that it is difficult to place and take out the centrifugal microfluidic chip in the microfluidic chip positioning tray is avoided.

[0185] 6. The centrifugal microfluidic chip clamping device, in which the centrifugal microfluidic chip can be directly placed in the microfluidic chip positioning tray for centrifugation, and no additional rotating shaft mounting hole is needed to be opened in the center of the centrifugal microfluidic chip, so that compared with the existing microfluidic chip hole with a rotating shaft mounting hole in the center, the centrifugal microfluidic chip clamping device of the application is applicable to the centrifugal microfluidic chip which is reduced in size while ensuring that the channel length of the centrifugal microfluidic chip will not be too small.

[0186] The application will be further described in conjunction with specific examples. It should be understood that these examples are only used to illustrate the application and not used to limit the scope of the application. The experimental methods in the following examples are not specified, and the general conditions or the conditions recommended by the manufacturer are usually used. Unless otherwise specified, percentages and parts are calculated by weight.

[0187] Example 1

[0188] Example 1 provides a centrifugal microfluidic chip, and a structural schematic diagram of the centrifugal microfluidic chip is shown in Figures 1-4 The centrifugal microfluidic chip includes a circular channel disc 2.1, and the channel disc is provided with a plurality of liquid storage cavities 2.2.

[0189] The center area of the channel disc is provided with a circular reagent cavity, each liquid storage cavity is in communication with the circular reagent cavity 2.3, each liquid storage cavity is uniformly arranged around the circular reagent cavity, the circular reagent cavity is protruded on the channel disc, the center of the channel disc is coincided with the center of the circular reagent cavity, each liquid storage cavity is connected with a main microchannel 2.4, the main microchannel is distributed along the center of the channel disc to the outer periphery of the channel disc, the distal end of the main microchannel away from the liquid storage cavity is communicated with a first exhaust cavity through a main hole 2.5, and one end of the main microchannel close to the main hole is provided with a left microchannel 2.6 and a right microchannel 2.7, the distal end of the left microchannel and the right microchannel is communicated with a second exhaust cavity and a third exhaust cavity through a left hole 2.8 and a right hole 2.9 respectively.

[0190] The channel disc is provided with two liquid adding ports 2.10, the liquid adding port is communicated with the liquid storage cavity, and the liquid adding port is provided with a glue film 2.18.

[0191] The first exhaust cavity comprises a first exhaust passage 2.12 and a first excess reagent cavity 2.13, the second exhaust cavity comprises a second exhaust passage 2.14 and a second excess reagent cavity 15, and the third exhaust cavity comprises a third exhaust passage 2.16 and a third excess reagent cavity 2.17, and the first excess reagent cavity, the second excess reagent cavity and the third excess reagent cavity are respectively provided with an exhaust hole 2.11; the main channel communicates with the first excess reagent cavity through the first exhaust passage, the left channel communicates with the second excess reagent cavity through the second exhaust passage, and the right channel communicates with the third excess reagent cavity through the third exhaust passage;

[0192] The hydrophilicity of each main microchannel is less than that of the liquid storage cavity connected thereto, and the hydrophilicity of the main microchannel, the left microchannel and the right microchannel is the same;

[0193] The main microchannel, the left microchannel and / or the right microchannel are straight channels, the longitudinal section of the left microchannel is a rectangle with a length of 0.12 mm and a width of 0.04 mm, the longitudinal section of the main microchannel is a rectangle with a length of 0.08 mm and a width of 0.4 mm, and the longitudinal section of the right microchannel is a rectangle with a length of 0.12 mm and a width of 0.04 mm;

[0194] The included angle α1 between the left microchannel and the main microchannel is 56°, and the included angle α2 between the right microchannel and the main microchannel is 56°;

[0195] The channel disc is further provided with a positioning hole 2.19.

[0196] Example 1: Gel filling process of centrifugal microfluidic chip

[0197] Gel is added through the liquid inlet of the channel disc, and after the gel fills each liquid storage cavity and the circular reagent cavity, the centrifugal microfluidic chip is placed in a centrifugal device, centrifuged, and the gel in the liquid storage cavity and the circular reagent cavity is filled into the corresponding main microchannel, left microchannel and right microchannel. After the gel fills the main microchannel, the left microchannel and the right microchannel, the gel almost simultaneously enters the first exhaust cavity, the second exhaust cavity and the third exhaust cavity through the main channel, the left channel and the right channel, that is, when the first exhaust cavity, the second exhaust cavity and the third exhaust cavity appear gel, it indicates that the main microchannel, the left microchannel and the right microchannel are filled with gel, and the centrifugation is stopped. The microfluidic chip with the main microchannel, the left microchannel and the right microchannel filled with gel is obtained, thereby realizing one-time feeding and one-time centrifugation, i.e. fast and accurate filling of multiple channels of gel, and the gel filled in the main microchannel, the left microchannel and the right microchannel is free of bubbles, ensuring the quality of gel filling.

[0198] Example 1: DNA electrophoresis experiment of centrifugal microfluidic chip

[0199] When the main microchannel, the left microchannel and the right microchannel of the centrifugal microfluidic chip of Example 1 are filled with gel, the electrodes of the instrument simultaneously puncture the left channel, the main channel and the right channel; then the instrument automatically adds the DNA sample to be tested into the left channel, and then the electrodes of the instrument are inserted into the left channel and the right channel, the left channel is connected to a negative electrode, and the right channel is connected to a positive electrode. Since the DNA sample is negatively charged, the sample will flow through the intersection of the main microchannel and the left microchannel and the right microchannel under the action of the Lorentz force. The electrodes are disconnected, and then the electrodes are inserted into the main channel and the liquid inlet, the liquid inlet is connected to a positive electrode, and the main channel is connected to a negative electrode. Under the action of the Lorentz force, the sample at the intersection of the left channel, the main channel and the right channel flows into the main microchannel. The sample combines with the pre-coated reagent in the main microchannel, and under the irradiation of a specific wavelength of laser, a specific wavelength is generated. The wavelength is received by the optical sensor, and under the processing of the software, a corresponding graph is produced. The tester can effectively determine the sample according to the graph.

[0200] Comparative Example 1

[0201] Comparative Example 1 provides a centrifugal microfluidic chip. The centrifugal microfluidic chip of Comparative Example 1 is similar in structure to the centrifugal microfluidic chip of Example 1. The centrifugal microfluidic chip of Comparative Example 1 is different from the centrifugal microfluidic chip of Example 1 in that in the centrifugal microfluidic chip of Comparative Example 1, the hydrophilicity of part of the main microchannels is equal to or greater than the hydrophilicity of the liquid storage cavities connected thereto.

[0202] During the gel filling process of the centrifugal microfluidic chip of Comparative Example 1, it cannot be ensured that the gel can simultaneously fill all the main microchannels, left microchannels and right microchannels during centrifugation. These part of the main microchannels (the hydrophilicity is equal to or greater than the hydrophilicity of the liquid storage cavities connected thereto) will enter the first exhaust cavity, the second exhaust cavity and the third exhaust cavity in advance before or during centrifugation, and even be discharged from the exhaust hole in advance to pollute the instrument, resulting in failure of gel filling.

[0203] Comparative Examples 2 and 3

[0204] Comparative Example 2 provides a centrifugal microfluidic chip. The centrifugal microfluidic chip of Comparative Example 2 is similar in structure to the centrifugal microfluidic chip of Example 1. The centrifugal microfluidic chip of Comparative Example 2 is different from the centrifugal microfluidic chip of Example 1 in that in the centrifugal microfluidic chip of Comparative Example 2, the included angle α1 between the left microchannel and the main microchannel is 54°, and the included angle α2 between the right microchannel and the main microchannel is 54°.

[0205] The comparative example 3 provides a centrifugal microfluidic chip. The centrifugal microfluidic chip of the comparative example 3 is similar in structure to the centrifugal microfluidic chip of the example 1. The centrifugal microfluidic chip of the comparative example 3 is different from the centrifugal microfluidic chip of the example 1 in that the angle a1 between the left microchannel and the main microchannel is 58°, and the angle a2 between the right microchannel and the main microchannel is 58°.

[0206] During the gel filling process of the centrifugal microfluidic chips of the comparative example 2 and the comparative example 3, the gel cannot enter the first exhaust cavity, the second exhaust cavity and the third exhaust cavity at the same time. When the exhaust holes of some of the first exhaust cavities, the second exhaust cavities and the third exhaust cavities are discharged (gel discharge from the exhaust holes will pollute the centrifugal microfluidic chip and the centrifugal device, and stop centrifugation), while the remaining other first exhaust cavities, second exhaust cavities and third exhaust cavities do not appear gel, resulting in the main microchannel, the left microchannel and the right microchannel appear not filled with gel phenomenon, so as to not meet the requirement of filling all the main microchannel, the left microchannel and the right microchannel, and the filling of the gel fails.

[0207] Example 2

[0208] The example 2 provides a centrifugal microfluidic chip clamping device. The device comprises a centrifugal microfluidic chip positioning part and a transmission part.

[0209] The centrifugal microfluidic chip positioning part comprises a centrifugal microfluidic chip positioning tray 1.1, a centrifugal pressure hand shaft 1.2 and a centrifugal pressure hand 1.3. The centrifugal pressure hand is provided with a through hole 1.4. The centrifugal pressure hand is a "Z" type centrifugal pressure hand. The "Z" type centrifugal pressure hand comprises a pressure hand head 1.6, a pressure hand body 1.7 and a pressure hand tail 1.8. The pressure hand head and the pressure hand body form an angle of 105°. The pressure hand tail and the pressure hand body form an angle of 95°. The angle formed by the pressure hand head and the pressure hand body is opposite to the angle formed by the pressure hand tail and the pressure hand body. The angle formed by the pressure hand head and the pressure hand body is towards the microfluidic chip positioning tray.

[0210] The outer periphery of the centrifugal microfluidic chip positioning tray is provided with 3 hanging through holes 1.5 and 2 positioning pins. The centrifugal pressure hand shaft passes through the through hole of the centrifugal pressure hand. The two ends of the centrifugal pressure hand shaft pass through the hanging through holes. The centrifugal pressure hand can rotate around the centrifugal pressure hand shaft.

[0211] In the non-centrifugal state, the centrifugal pressure hand is in a hanging state. The center of gravity of the centrifugal pressure hand is below the through hole.

[0212] In the centrifugal state, the centrifugal pressure hand can rotate around the centrifugal pressure hand shaft with the lower end outward and upward, and the upper end inward and downward.

[0213] The transmission part comprises a motor 1.10 and a rotating shaft 1.11, the rotating shaft is connected with the motor, the microfluidic chip positioning tray is connected with the rotating shaft, and the transmission part can drive the centrifugal microfluidic chip positioning tray to rotate;

[0214] The centrifugal microfluidic chip positioning tray is further provided with a pressing hole 1.9, a long strip-shaped hole and a mounting groove, the pressing hole is used for pressing the centrifugal pressing shaft, the centrifugal pressing shaft is arranged on the mounting groove, and the detection equipment can detect the centrifugal microfluidic chip through the long strip-shaped hole of the centrifugal microfluidic chip positioning tray.

[0215] Figure 6 and Figure 7 respectively are structural schematic diagrams of the centrifugal microfluidic chip positioning tray and the centrifugal pressing hand, Figure 8 and Figure 9 are structural schematic diagrams of the centrifugal microfluidic chip clamping device provided with the centrifugal microfluidic chip, Figure 10 and Figure 11 respectively are states of the centrifugal pressing hand in non-centrifugal and centrifugal states.

[0216] The working principle of the microfluidic chip clamping device in Example 2 mainly comprises:

[0217] In the non-centrifugal state, the centrifugal pressing hand is in a suspended state (as shown in Figure 10 ), and the centrifugal microfluidic chip is conveniently placed on the centrifugal microfluidic chip positioning tray, the positioning pin can be combined with the positioning hole of the centrifugal microfluidic chip positioning tray, and is used for positioning the centrifugal microfluidic chip to prevent confusion; when the motor drives the centrifugal microfluidic chip positioning tray to rotate at high speed through the rotating shaft, under the action of centrifugal force, the centrifugal pressing hand can rotate from the lower end outward and upward to the upper end inward (as shown in Figure 11 ), so that the centrifugal pressing hand clamps the centrifugal microfluidic chip and generates pressure on the centrifugal microfluidic chip, preventing the centrifugal microfluidic chip from falling off from the microfluidic chip positioning tray during centrifugation. The greater the rotation speed of the centrifugal microfluidic chip positioning tray, the greater the centrifugal force, the stronger the rotation of the centrifugal pressing hand, the greater the pressure generated on the centrifugal microfluidic chip, and the centrifugal microfluidic chip is prevented from falling off due to high-speed centrifugation.

[0218] Example 3

[0219] Example 3 provides a gel electrophoresis device, which is prepared from the centrifugal microfluidic chip prepared in Example 1 and the centrifugal microfluidic chip clamping device prepared in Example 2.

[0220] The positioning pin on the centrifugal micro-fluidic chip positioning tray is combined with the positioning hole of the centrifugal micro-fluidic chip, so that the centrifugal micro-fluidic chip is installed on the centrifugal micro-fluidic chip positioning tray (as shown in Figure 8 or Figure 9 After the gel is added through the liquid inlet of the channel disc, when the motor drives the centrifugal micro-fluidic chip positioning tray to rotate at high speed through the rotating shaft, the main micro-channel, the left micro-channel and the right micro-channel on the centrifugal micro-fluidic chip are filled with the gel at one time under the action of centrifugal force, and the DNA electrophoresis experiment is carried out.

[0221] All the documents mentioned in the present application are cited as references in the present application, as if each document is cited as a reference individually. In addition, it should be understood that, after reading the above teaching of the present application, those skilled in the art can make various modifications or amendments to the present application, and these equivalent forms also fall within the scope defined by the claims attached to the present application.

Claims

1. A centrifugal microfluidic chip, characterized by, The centrifugal microfluidic chip comprises a channel disc (2.1); The channel disc is provided with a plurality of liquid storage cavities (2.2), a circular reagent cavity is arranged in the central region of the channel disc, each liquid storage cavity is communicated with the circular reagent cavity (2.3), each liquid storage cavity is connected with a main microchannel (2.4), the end of the main microchannel away from the liquid storage cavity is communicated with a first exhaust cavity through a main hole (2.5), one end of the main microchannel close to the main hole is provided with a left microchannel (2.6) and a right microchannel (2.7), and the ends of the left microchannel and the right microchannel are communicated with a second exhaust cavity and a third exhaust cavity through a left hole (2.8) and a right hole (2.9) respectively; The channel disc is provided with a liquid adding port (2.10), the liquid adding port is communicated with the liquid storage cavity or the circular reagent cavity, and the first exhaust cavity, the second exhaust cavity and the third exhaust cavity are respectively provided with exhaust holes (2.11); The liquid storage cavity, the circular reagent cavity, the main microchannel, the left microchannel and / or the right microchannel are arranged on the lower surface side of the channel disc; The first exhaust cavity, the second exhaust cavity and / or the third exhaust cavity are arranged on the upper surface side of the channel disc; The hydrophilicity of the main microchannel is smaller than that of the liquid storage cavity; The channel disc is further provided with a positioning hole (2.19), and the positioning hole is used in combination with a positioning pin on a centrifugal microfluidic chip clamping device; The included angle α1 between the left microchannel and the main microchannel is 56±0.5°; and / or The included angle α2 between the right microchannel and the main microchannel is 56±0.5°.

2. The centrifugal microfluidic chip of claim 1 wherein, The number of the liquid storage cavities is 2-60.

3. The centrifugal microfluidic chip of claim 1 wherein, The main microchannel is made of a hydrophobic material or is subjected to hydrophobic treatment on the surface.

4. The centrifugal microfluidic chip of claim 1 wherein, Each liquid storage cavity is uniformly arranged around the circular reagent cavity; and / or The center of the channel disc coincides with the center of the circular reagent cavity.

5. The centrifugal microfluidic chip of claim 1 wherein, The main microchannel is distributed along the center of the channel disc to the outer periphery of the channel disc.

6. The centrifugal microfluidic chip of claim 1 wherein, The area S1 of the longitudinal section of the main microchannel is 0.002 mm 2 ≤ S1 ≤ 0.005 mm 2 ; and / or The area S2 of the longitudinal section of the left microchannel is 0.003 mm 2 ≤ S2 ≤ 0.007 mm 2 ; and / or The area S3 of the longitudinal section of the right microchannel is 0.003 mm 2 ≤ S3 ≤ 0.007 mm 2 .

7. The centrifugal microfluidic chip of claim 1 wherein, The area S2 of the longitudinal section of the left microchannel is 0.8-2 times the area S1 of the longitudinal section of the main microchannel; and / or The area S3 of the longitudinal section of the right microchannel is 0.8-2 times the area S1 of the longitudinal section of the main microchannel.

8. The centrifugal microfluidic chip of claim 1 wherein, The included angle α1 between the left microchannel and the main microchannel is equal to the included angle α2 between the right microchannel and the main microchannel.

9. The centrifugal microfluidic chip of claim 1 wherein, Each liquid storage cavity is a long-hole type liquid storage cavity, the dimensional tolerance of the long-hole type liquid storage cavity is 0.015-0.025 mm, and the depth is 4.8-5.2 mm.

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