Biochemical and Immunological Integrated Analyzer and Detection Method

Abstract

The present invention belongs to the technical field of biochemical and immunological integrated analyzers, and provides a biochemical and immunological integrated analyzer. By arranging a first incubation tank module including a plurality of first operation positions along a straight line, driving a first chip cartridge with a disposable test reagent sealed inside and a side wall that can block light from entering the cartridge body to move along the first operation positions through the first incubation tank module for chemiluminescence detection, arranging a second incubation tank module including a plurality of second operation positions along a straight line, driving a second chip cartridge with a disposable test reagent sealed inside and a side wall that can transmit light to move along the second operation positions through the second incubation tank module for biochemical detection, and then arranging an operation mechanism beside the first operation positions and the second operation positions to perform corresponding operations, thereby effectively avoiding the problem of the expiration date after opening the bottle and the operation of the "calibration" process, so that the detection process of the biochemical and immunological integrated analyzer with the above-mentioned incubation tank module is instant and simple, and the detection accuracy is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biochemical and immunological integrated analyzers, and particularly relates to a biochemical and immunological integrated analyzer and a detection method. Background Art

[0002] Currently, an annular incubation tank is often used in a biochemical and immunological integrated analyzer to place reagent bottles and reaction cups. Among them, the reagent bottles are filled with reagent amounts for multiple persons to be used for detecting multiple samples. During the detection of a specific sample, the reagent bottle is opened, and then a reagent suction device is used to suck the reagent and mix it with the sample in the reaction cup for detection. Summary of the Invention

[0003] Although the annular incubation tank of the existing biochemical and immunological integrated analyzer can realize sample detection, however, since the market positioning of the existing biochemical and immunological integrated analyzer with an annular incubation tank is usually for large medical institutions, the annular incubation tank needs to be able to place multiple reaction cups and reagent bottles filled with a large amount of reagent measurement to meet the requirements of multiple-person testing. However, it is found in practice that the reagent in the reagent bottle usually cannot be used up after opening, thus causing the problem of the opening validity period, that is, when the remaining reagent after opening is used again after a certain period of time, it may cause a reduction in the detection accuracy of the sample. In addition, since the reaction cups and reagent bottles in the annular incubation tank exist independently, it is usually an important reason for the need to go through a complex "calibration" process during sample detection. "Calibration" requires professional operation and the operation process is complex, so that it is difficult to popularize the biochemical and immunological integrated analyzer with an annular incubation tank in small medical institutions such as community hospitals.

[0004] In summary, the annular incubation tank of the existing biochemical and immunological integrated analyzer has defects such as causing complex detection procedures of the biochemical and immunological integrated analyzer and being prone to reducing the detection accuracy.

[0005] The present invention provides a biochemical and immunological integrated analyzer to solve the above defects of the annular incubation tank of the existing analyzer. The biochemical and immunological integrated analyzer includes:

[0006] A first incubation tank module, including a plurality of first operation positions arranged in a straight line, for driving a first chip box sealed with a disposable test reagent to move along the first operation positions for chemiluminescence detection;

[0007] A second incubation tank module, including a plurality of second operation positions arranged in a straight line, for driving a second chip box sealed with a disposable test reagent to move along the second operation positions for biochemical detection;

[0008] An operating mechanism, arranged beside the first operation positions and the second operation positions for corresponding operations.

[0009] Specifically, the first incubation tank module includes a first incubation tank having a first linear channel inside; above the first incubation tank, a first operation position communicating with the first linear channel is arranged along the direction of the first linear channel; on one side of the first linear channel, a first driving mechanism capable of driving the first chip cartridge to pass through is arranged.

[0010] Specifically, the second incubation tank module includes a second incubation tank having a second linear channel inside; above the second incubation tank, a second operation position communicating with the second linear channel is arranged along the direction of the second linear channel; on one side of the second linear channel, a second driving mechanism capable of driving the second chip cartridge to pass through is arranged.

[0011] Specifically, the first operation position includes a first detection position, a first sample addition position, a first puncture position, and a magnetic separation and cleaning position that are successively farther away from the entrance of the first chip cartridge.

[0012] Specifically, the second operation position includes a second sample addition position, a second puncture position, a second detection position, and a second chip cartridge identification position that are successively farther away from the entrance of the second chip cartridge.

[0013] Specifically, the operating mechanism includes a sample injection mechanism arranged beside the first incubation tank module or the second incubation tank module; the sample injection mechanism includes a sample driving device for transporting the sample tube to move linearly, an identification device for identifying the identity label of the sample tube on one side of the sample driving device, and a monitoring device for detecting the presence or absence of the sample tube on one side of the sample driving device.

[0014] Specifically, the operating mechanism includes a sampling mechanism arranged above the first incubation tank module or the second incubation tank module; the sampling mechanism includes a lateral driving device installed on a vertical plate and a vertical sampling and sample placing device that can be driven by the lateral driving device to translate.

[0015] Specifically, the operating mechanism includes a first puncture mechanism arranged above the first puncture position; the first puncture mechanism includes a first puncture driving device installed on a first cross beam and a first puncture needle that can be driven by the first puncture driving device to puncture downward.

[0016] Specifically, the operating mechanism includes a second puncture mechanism arranged above the second puncture position; the second puncture mechanism includes a second puncture driving device installed on a second cross beam and a second puncture needle that can be driven by the second puncture driving device to puncture downward.

[0017] The present invention also provides a detection method, including:

[0018] Arranging a first incubation tank module including a plurality of first operation positions along a straight line;

[0019] Through the first incubation tank module, drive the first chip cartridge containing a disposable test reagent along the first operation position to perform chemiluminescence detection;

[0020] A second incubation tank module including a plurality of second operation positions is arranged in a straight line;

[0021] Through the second incubation tank module, drive the second chip cartridge containing a disposable test reagent along the second operation position to perform biochemical detection;

[0022] Layout an operating mechanism beside the first operation position and the second operation position to perform corresponding operations.

[0023] The biochemical and immuno integrated analyzer provided by the present invention drives the first chip cartridge containing a disposable test reagent and with side walls capable of blocking light from entering the cartridge body along the first operation position through the first incubation tank module including a plurality of first operation positions arranged in a straight line, drives the second chip cartridge containing a disposable test reagent and with side walls capable of transmitting light along the second operation position through the second incubation tank module including a plurality of second operation positions arranged in a straight line, and then layouts an operating mechanism beside the first operation position and the second operation position to perform corresponding operations, thereby effectively avoiding the problems of the opening validity period and the operation of the "calibration" process, and making the detection process of the biochemical and immuno integrated analyzer with the above-mentioned incubation tank module instant and simple and the detection accuracy improved. Description of the Drawings

[0024] Figure 1 It is a schematic structural diagram of a biochemical and immuno integrated analyzer provided by an embodiment;

[0025] Figure 2 It is a schematic structural diagram of the first incubation tank module provided by an embodiment;

[0026] Figure 3 It is a schematic structural diagram of a clamping mechanism provided by an embodiment;

[0027] Figure 4 It is a schematic structural diagram of the second incubation tank module provided by an embodiment;

[0028] Figure 5 It is a schematic structural diagram of a sample injection mechanism provided by an embodiment;

[0029] Figure 6 It is a schematic structural diagram of a sampling mechanism provided by an embodiment;

[0030] Figure 7 It is a schematic structural diagram of a puncture mechanism provided by an embodiment;

[0031] Figure 8 Schematic structural diagram of a puncture mechanism provided for an embodiment;

[0032] Figure 9 Schematic structural diagram of a light-emitting detection mechanism provided for an embodiment;

[0033] Figure 10 Schematic structural diagram of an optical detection mechanism provided for an embodiment;

[0034] Figure 11 Schematic structural diagram of a liquid suction and cleaning mechanism provided for an embodiment;

[0035] Figure 12 Schematic structural diagram of a liquid-electric unit provided for an embodiment;

[0036] Figure 13 Schematic flow diagram of a detection method provided for an embodiment. Detailed implementation manners

[0037] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0038] In addition, in the description of the present invention, unless otherwise clearly defined and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can also be the communication inside two elements. It can be a wireless connection or a wired connection. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0039] In addition, the technical features involved in different embodiments of the present invention described hereinafter can be combined with each other as long as they do not conflict with each other.

[0040] Next, the present invention proposes some preferred embodiments to teach those skilled in the art to implement.

[0041] Figure 1Schematic diagram of the structure of a biochemical and immuno integrated analyzer provided for an embodiment, showing a biochemical and immuno integrated analyzer, which includes: a first incubation tank module b, a second incubation tank module c, and an operating mechanism arranged on the bottom plate a. The first incubation tank module b and the second incubation tank module c are arranged side by side, and the operating mechanism is arranged beside the first operating position and the second operating position for corresponding operations.

[0042] On the one hand, the first incubation tank module b includes a plurality of first operating positions arranged in a straight line, which are used to drive the first chip cartridge containing a disposable test reagent and having side walls that can block light from entering the cartridge body to move along the first operating positions. Among them, the side walls of the chip cartridge being able to block light is an implementation method of chemiluminescence detection. Chemiluminescence detection is a specific detection method, that is, a method that requires blocking light from passing through the side walls of the chip cartridge to irradiate the sample for detection. Therefore, the part of the side wall of the first chip cartridge opposite to the second detection position needs to have light-shielding properties so that light cannot pass through and irradiate the sample to be detected.

[0043] On the other hand, the second incubation tank module c includes a plurality of second operating positions arranged in a straight line, which are used to drive the second chip cartridge containing a disposable test reagent and having side walls that are light-transmissive to move along the second operating positions. Among them, the side walls of the chip cartridge being light-transmissive is an implementation method of biochemical detection. Biochemical detection is a specific detection method, that is, a method that irradiates the sample by means of light passing through the side walls of the chip cartridge for detection. Therefore, the part of the side wall of the second chip cartridge opposite to the second detection position needs to be light-transmissive so that light can pass through and irradiate the sample to be detected.

[0044] On the other hand, the operating mechanism includes a sample injection mechanism 7, a sampling mechanism 5, a first puncture mechanism b3, a second puncture mechanism 4, a liquid suction and cleaning mechanism b4, a motion pump mechanism 13, a luminescence detection mechanism b2, an optical detection mechanism 3, etc.

[0045] Among them, the sample injection mechanism 7 can be arranged side by side on one side of the first incubation tank module b, or can be arranged side by side on one side of the second incubation tank module c, or can also be arranged side by side between the first incubation tank module b and the second incubation tank module c.

[0046] It should be noted that since both the first incubation tank module b and the second incubation tank module c are in a straight-line structure, and the sample injection mechanism 7 is arranged side by side with the first incubation tank module b and the second incubation tank module c, the effect of one sample injection mechanism feeding samples for two incubation tank modules can be achieved, which not only saves space, reduces the overall volume of the biochemical and immuno integrated analyzer, but also saves the sample injection cost and improves the sample injection efficiency.

[0047] In addition, the sampling mechanism 5 can be arranged above the first incubation tank module b and the second incubation tank module c, and through two-dimensional movement in the horizontal and vertical directions, sampling of the first incubation tank module b and the second incubation tank module c can be completed. For example, sucking reagents.

[0048] It should be noted that since one sampling mechanism can sample the first incubation tank module b and the second incubation tank module c, it not only saves space, reduces the overall volume of the biochemical immune integrated analyzer, but also saves the sampling cost and improves the sampling efficiency.

[0049] In addition, the liquid suction and cleaning mechanism b4 is arranged above the first incubation tank module b, and can perform relevant liquid suction and cleaning on the first incubation tank module b.

[0050] In addition, the motion pump mechanism 13 is arranged above the first incubation tank module b, and can provide power for the liquid flow in the liquid path.

[0051] On the other hand, a cleaning pool device f can be arranged between the first incubation tank module b and the second incubation tank module c for cleaning the operating mechanisms that need to be cleaned. For example, cleaning the first puncture mechanism b3, the second puncture mechanism 4 or the sampling mechanism 5.

[0052] It should be noted that since one cleaning pool device can clean multiple mechanisms that need liquid exchange or cleaning, it not only saves space, reduces the overall volume of the biochemical immune integrated analyzer, but also saves the sampling cost and improves the cleaning efficiency.

[0053] In this embodiment, by arranging a first incubation tank module including a plurality of first operating positions in a straight line, driving a first chip cartridge with a disposable test reagent sealed inside and a side wall that can block light from entering the cartridge body to move along the first operating position through the first incubation tank module, by arranging a second incubation tank module including a plurality of second operating positions in a straight line, driving a second chip cartridge with a disposable test reagent sealed inside and a side wall that can transmit light to move along the second operating position through the second incubation tank module, and then arranging operating mechanisms beside the first operating position and the second operating position to perform corresponding operations, thereby effectively avoiding the generation of the open bottle expiration date problem and the operation of the "calibration" process, so that the detection process of the biochemical immune integrated analyzer with the above-mentioned incubation tank module is instant and simple and the detection accuracy is improved.

[0054] It should be noted that in the prior art, a chemiluminescence immunoassay device with a circular incubation tank structure has multiple different operation positions. For example, a puncture position, a sample addition position, a second detection position, etc. However, these workstations are all arranged around the periphery of the disc. Correspondingly, the operating mechanisms for completing the puncture operation, the sample addition operation, and the detection operation are correspondingly arranged around multiple different operation positions. The reason why the prior chemiluminescence immunoassay device sets its incubation tank assembly into a circular structure is to perform sample analysis and detection of multiple samples and multiple specimens. Although the prior chemiluminescence immunoassay device can complete the sample analysis and detection of multiple samples and multiple specimens, it has defects such as complex analysis and detection operations of the device and reduced detection accuracy. The biochemical and immuno integrated analyzer provided in this embodiment brings about a fundamental change in the overall structure of the instrument based on the improvement of the incubation tank module, making the detection process of the biochemical and immuno integrated analyzer with this incubation tank module instantaneously simple and the detection accuracy improved, so that the instrument can truly meet the needs of small medical institutions such as community hospitals for instant, simple, and single-sample detection.

[0055] It should also be noted that since the sample detection is completed by different operation positions arranged linearly on the incubation tank module, the operating mechanisms such as the sample injection mechanism, the sampling mechanism, the puncture mechanism, and the luminescence detection mechanism on the incubation tank module can be arranged along the straight line where the different operation positions are located, so that the overall structure of the instrument is concise and compact.

[0056] It should further be noted that the biochemical and immuno integrated analyzer provided with the first incubation tank module and the second incubation tank module in this embodiment does not mean that the present invention only includes two incubation tank modules. According to the demand for instant single-sample detection, multiple incubation tank modules can also be set up as needed.

[0057] Figure 2 It is a schematic structural diagram of the first incubation tank module provided in an embodiment, showing an incubation tank module.

[0058] See Figure 1-2 , the first incubation tank module b includes a first incubation tank b100 with a linear channel inside; along the direction of the linear channel on the upper surface of the first incubation tank b100, a plurality of different first operation positions communicating with the linear channel are provided; on one side of the linear channel, a first driving mechanism 103 for driving a first chip box internally sealed with a disposable test reagent to pass is arranged; the side wall of the first chip box can block light from entering the box body.

[0059] Among them, the first incubation tank b100 can be in a strip structure. The operation positions can include a first puncture position b108, a first sample addition position b110, a magnetic separation and cleaning position b106, a first detection position b111, etc. The setting order of the operation positions such as the first puncture position b108, the first sample addition position b110, the magnetic separation and cleaning position b106, and the first detection position b111 on the first incubation tank b100 can be set according to specific needs. Preferably, the distances of the first detection position b111, the first sample addition position b110, the first puncture position b108, and the magnetic separation and cleaning position b106 from the chip cassette entrance gradually become farther.

[0060] Improved, a first in-place sensor b112 for sensing the position information of the chip cassette is further provided on the upper surface of the first incubation tank b100; the first in-place sensor b112 is arranged near the first chip cassette entrance b116 of the linear channel. Preferably, the distances of the first in-place sensor b112, the first detection position b111, the first sample addition position b110, the first puncture position b108, and the magnetic separation and cleaning position b106 from the first chip cassette entrance b116 gradually become farther.

[0061] Improved, a first chip cassette identification position b113 is provided between the first in-place sensor b112 and the first chip cassette entrance b116; the first chip cassette identification position b113 communicates with the linear channel. Among them, the first chip cassette identification position b113 can identify the identification information (such as a two-dimensional code) of the first chip cassette, and can confirm the identity information of the first chip cassette, thereby avoiding errors in the detection object. For example, the identification information of the first chip cassette can be scanned and obtained by supporting a scanner b114 above the first incubation tank b100 through a scanner support b115 for comparison with the background.

[0062] Improved, a puncture fixation position 109 is provided beside the first puncture position b108, and a liquid suction fixation position 107 is provided beside the magnetic separation and cleaning position b106. Among them, the puncture fixation position 109 and the liquid suction fixation position 107 can respectively play the roles of puncture fixation and magnetic attraction fixation.

[0063] In this embodiment, by arranging a linear channel inside the first incubation tank b100, arranging different operation positions communicating with the linear channel along the direction of the linear channel on the upper surface of the first incubation tank b100, arranging a first driving mechanism 103 on one side of the linear channel, and then by arranging a first chip cassette with side walls that can block light from entering the box body, with a disposable test reagent sealed in the first chip cassette, and then driving the first chip cassette to travel along the linear channel by the first driving mechanism 103, the generation of the open bottle expiration date problem and the operation of the "calibration" process are effectively avoided, so that the detection process of the biochemical analyzer equipped with the first incubation tank assembly b is simple and immediate, and the detection accuracy is improved.

[0064] It should be noted that since the test reagent sealed in the first chip cartridge is a single-use reagent for single-person use, the test reagent is pre-sealed in the first chip cartridge by a film with a single-use measurement. At the same time, the pre-sealed reagent has been calibrated through a special "calibration" process. When using the test reagent, there is no need to rely on a reagent "calibration" mechanism to perform complex "calibration" operations, thus making the overall volume of the biochemical analyzer equipped with the first incubation tank assembly b small and the detection process immediate and simple. Among them, because the test reagent is a sealed single-use reagent for single-person use, it can not only effectively avoid the problem of the expiration date after opening the bottle, and prevent the test accuracy from being reduced due to the reuse of the remaining reagent, but also avoid setting an additional reagent aspiration device to aspirate the reagent into the reaction cup, thus effectively reducing a reagent loading and unloading process and improving the detection efficiency.

[0065] In addition, when specifically using the first incubation tank assembly b of the biochemical immunoassay analyzer in this embodiment to detect and analyze a sample, the first chip cartridge is placed into the first chip cartridge inlet b116 of the linear channel, and then the first chip cartridge is driven to travel in the linear channel by the first driving mechanism 103, and the first puncture position b108 punctures the film of the first chip cartridge, and the first sample addition position b110 adds the sample to the punctured first chip cartridge, and then the first detection position b111 detects the chemiluminescence value of the reactant after mixing the sample and the reagent, so that an immediate, efficient and simple sample test can be realized.

[0066] In addition, compared with the biochemical immunoassay analyzer in the prior art that uses an annular incubation tank to place reagent bottles and reaction cups, and the reagent bottles are filled with reagent amounts for multiple-person use to detect multiple samples, the biochemical immunoassay analyzer with the first incubation tank assembly b provided in this embodiment has the advantages of immediate and simple detection process and relatively high detection accuracy.

[0067] It should also be noted that the long-strip-shaped first incubation tank b100 is conducive to setting a linear channel, and is conducive to setting the first puncture position b108, the first sample addition position b110, the magnetic separation and cleaning position b106, and the first detection position b111 along the linear channel, so that other mechanisms of the biochemical immunoassay analyzer can be arranged along the long-strip-shaped first incubation tank b100, thereby achieving the effect of concentrating the mechanism positions to reduce the overall volume of the biochemical immunoassay analyzer.

[0068] It should also be noted that when detecting the chemiluminescence value of the reactant, it is necessary to avoid natural light from irradiating the reactant in the first chip cartridge. Therefore, the side wall of the first chip cartridge needs to be able to block light from entering the cartridge body. Specifically, the first chip cartridge can be made of light-impermeable materials.

[0069] It should also be noted that the first incubation tank b100 has the functions of storing reactants at a constant temperature and providing a suitable reaction temperature, which can be specifically composed of a heating device, a temperature sensor, a temperature regulating device, etc.

[0070] Figure 3 FIG. is a schematic structural diagram of a clamping mechanism provided for an embodiment, showing a clamping mechanism in the first incubation tank assembly b.

[0071] See Figure 1-3 , the first driving mechanism 103 drives the first chip cassette to pass by driving a first clamping and limiting mechanism to move. The first clamping and limiting mechanism includes: a first slider 101 connected to the first driving mechanism 103, a first clamping mechanism 104 that clamps the first chip cassette on one side of the first slider 101, and a first limiting device 102 that limits the movement of the first chip cassette on one side of the first driving mechanism 103.

[0072] Among them, the first clamping mechanism 104 includes a first clamping plate cover 1041, a first middle partition 1042, a first rotary hook extraction pin 1043, a first fixed hook 1044, a first side partition 1045, and a first bottom cover 1046. The first middle partition 1042, the first rotary hook extraction pin 1043, the first fixed hook 1044, and the first side partition 1045 are located between the first clamping plate cover 1041 and the first bottom cover 1046. The first rotary hook extraction pin 1043 and the first fixed hook 1044 are located between the first middle partition 1042 and the first bottom cover 1046, and can cooperate to clamp and release the first chip cassette.

[0073] It should be noted that the limiting clamping mechanism and the clamping mechanism in the second incubation tank assembly c are the same as those in the first incubation tank assembly b. To avoid repeated description, they will not be elaborated in this article.

[0074] Figure 4 FIG. is a schematic structural diagram of a second incubation tank module provided for an embodiment, showing an incubation tank module.

[0075] See Figure 1 and Figure 4 , the second incubation tank module c includes a second incubation tank 205 with a linear channel inside; along the direction of the linear channel on the upper surface of the second incubation tank 205, a second puncture position 2052 and a second sample addition position 2051 communicating with the linear channel are arranged at intervals; on the side surface of the second incubation tank 205, second detection positions (2054, 2055) communicating with the linear channel are provided; on one side of the linear channel, a second driving mechanism 203 that can drive a second chip cassette 204 sealed with a disposable test reagent to pass is arranged.

[0076] Specifically, the second driving mechanism 203 drives the second chip cassette 204 to pass by driving the clamping and limiting mechanism. The clamping and limiting mechanism includes a limiting block 2020 and front and rear sliders (2021, 2023) connected by springs; the limiting block 2020 limits the second chip cassette 204; on one side of the front and rear sliders (2021, 2023), there is a rotating hook that can rotate to clamp the second chip cassette 204, and one side is connected to the second driving mechanism 203.

[0077] It should be noted that the second driving mechanism 203 drives the second chip cassette 204 to pass by driving the clamping and limiting mechanism, which can realize clamping the second chip cassette 204 for two-way transportation and precise positioning at the required position.

[0078] In addition, the second driving mechanism 203 can select linear second driving mechanisms 203 such as a conveyor belt module and a lead screw motor module to achieve the purpose of driving the second chip cassette 204 to pass back and forth along the linear channel.

[0079] It should also be noted that the structure of the limiting and clamping mechanism in the second incubation tank assembly c is the same as that in the first incubation tank assembly b. To avoid repeated description, it will not be elaborated in this article.

[0080] Improved, on one side of the second incubation tank 205, there is also a second in-place sensor 206 for sensing the position information of the second chip cassette 204. The second in-place sensor 206 can be arranged near the entrance of the second chip cassette 204 in the linear channel. On one side of the second incubation tank 205, there is also a second chip cassette identification position 2053, and the second chip cassette identification position 2053 communicates with the linear channel. Among them, the second in-place sensor 206 and the second chip cassette identification position 2053 can be arranged on the same side of the second incubation tank 205 as the second puncture position 2052 and the second sample addition position 2051, or can be arranged on different sides of the second incubation tank 205 from the second puncture position 2052 and the second sample addition position 2051.

[0081] Specifically, the distance between the second chip cassette identification bit 2053 and the entrance of the second chip cassette 204 in the linear channel can be farther than the distance between the second puncture bit 2052 and the entrance of the second chip cassette 204 in the linear channel, or can be closer than the distance between the second puncture bit 2052 and the entrance of the second chip cassette 204 in the linear channel. The distance between the second puncture bit 2052 and the entrance of the second chip cassette 204 in the linear channel can be farther than the distance between the second sample addition bit 2051 and the entrance of the second chip cassette 204 in the linear channel, or can be closer than the distance between the second sample addition bit 2051 and the entrance of the second chip cassette 204 in the linear channel. Between the second chip cassette identification bit 2053 and the second puncture bit 2052, a second detection bit (2054, 2055) can be set. The second detection bit (2054, 2055) includes a transmission detection bit 2054 and / or a scattering detection bit 2055, and the transmission detection bit 2054 and / or the scattering detection bit 2055 can be arranged side by side.

[0082] Preferably, with reference to the entrance of the second chip cassette 204 in the linear channel, the distances of the second in-place sensor 206, the second sample addition bit 2051, the second puncture bit 2052, the second detection bit (2054, 2055), and the second chip cassette identification bit 2053 from the entrance of the second chip cassette 204 in the linear channel become farther in sequence.

[0083] It should be noted that setting the second in-place sensor 206 near the entrance of the second chip cassette 204 in the linear channel can detect whether the second chip cassette 204 is in place in the first time, thereby triggering the progress of further processes. Setting the transmission detection bit 2054 and / or the scattering detection bit 2055 between the second chip cassette identification bit 2053 and the second puncture bit 2052 can establish an optical path to realize biochemical analysis of the sample. Setting the second chip cassette identification bit 2053 on one side of the second incubation tank 205 can identify the identification information (such as a two-dimensional code) of the second chip cassette 204, and further confirm the identity information of the second chip cassette 204, thereby avoiding errors in the detection object.

[0084] In this embodiment, by arranging the second in-place sensor 206, the second puncture bit 2052, the second sample addition bit 2051, and the second chip cassette identification bit 2053 communicating with the linear channel at intervals along the direction of the linear channel on the upper surface of the second incubation tank 205 having a linear channel inside, and arranging the second detection bit (2054, 2055) communicating with the linear channel on the side surface of the second incubation tank 205, and arranging the second driving mechanism 203 capable of driving the second chip cassette 204 containing a disposable test reagent to pass on one side of the linear channel, the problem of the open bottle expiration date and the operation of the "calibration" process are effectively avoided, so that the detection process of the biochemical immune integrated analyzer equipped with the second incubation tank 205 assembly is simple and immediate, and the detection accuracy is improved.

[0085] It should be noted that since the test reagent sealed in the second chip cartridge 204 is a single-use reagent for single-person use, the test reagent is pre-sealed in the second chip cartridge 204 through a film with a single-use measurement. At the same time, the pre-sealed reagent has been calibrated through a special "calibration" process. When using the test reagent, there is no need to rely on a reagent "calibration" mechanism to perform complex "calibration" operations, thereby making the whole biochemical immunoassay analyzer with the second incubation tank 205 assembly small in volume and simple in detection process. Among them, because the test reagent is a sealed single-use reagent for single-person use, it can not only effectively avoid the problem of the expiration date after opening the bottle, and prevent the test accuracy from being reduced due to the reuse of the remaining reagent, but also avoid setting an additional reagent aspiration device to aspirate the reagent into the reaction cup, thereby effectively reducing a reagent loading and unloading process and effectively reducing the volume of the biochemical immunoassay analyzer.

[0086] In addition, when specifically using the biochemical immunoassay analyzer in this embodiment to detect and analyze a sample, the second chip cartridge 204 is placed into the second chip cartridge 204 entrance of the linear channel, and then the second chip cartridge 204 is driven by the second driving mechanism 203 to pass through the linear channel, and the second puncture position 2052 punctures the film of the second chip cartridge 204, and the second sampling position 2051 adds the sample to the punctured second chip cartridge 204, and then the second detection positions (2054, 2055) analyze the biochemical reaction value of the reactant after mixing the sample and the reagent, so as to achieve an instant, efficient and simple sample test.

[0087] In addition, compared with the existing biochemical immunoassay analyzer that uses an annular incubation tank to place reagent bottles and reaction cups, and the reagent bottles are filled with reagent amounts for multiple-person use to detect multiple samples, the biochemical immunoassay analyzer with the incubation tank assembly provided in this embodiment has the advantages of simple and instant detection process and relatively high detection accuracy.

[0088] Specifically, the second incubation tank 205 can be set to a long strip structure. The second incubation tank 205 with a long strip structure is conducive to setting a linear channel, and is conducive to setting the second puncture position 2052, the second sampling position 2051 and the second detection positions (2054, 2055) along the linear channel, so that other mechanisms of the biochemical immunoassay analyzer can be set along the second incubation tank 205 with a long strip structure, thereby achieving the effect of concentrating the mechanism positions to reduce the overall volume of the biochemical immunoassay analyzer.

[0089] It should also be noted that since the second incubation tank 205 is provided with second detection positions (2054, 2055) communicating with the linear channel on its side, an optical path can be established to perform biochemical analysis on the sample. At the same time, the side wall of the second chip cassette 204 is irradiated with light through the optical path, and the light reacts with the sample after passing through the light-transmissive side wall, and then the light is detected by transmission turbidimetry and / or scattering turbidimetry, so as to obtain the sample detection result.

[0090] It should also be noted that the second incubation tank 205 has the functions of storing reactants at a constant temperature and providing a suitable reaction temperature, which can be specifically composed of a heating device, a temperature sensor, a temperature regulating device, etc.

[0091] Figure 5 The structural schematic diagram of the sampling mechanism provided for an embodiment shows a sampling mechanism.

[0092] See Figure 1 Figure 5 The operating mechanism includes a sampling mechanism 7 provided on one side of the first incubation tank assembly b or one side of the second incubation tank assembly c; the sampling mechanism 7 includes a sample driving device 701 for transporting the sample tube 7014 to the sampling station, an identification device 702 for identifying the identity label of the sample tube 7014 on one side of the sample driving device 701, and a monitoring device 703 for detecting the presence or absence of the sample tube 7014 on one side of the sample driving device 701.

[0093] Specifically, the sample driving device 701 includes: an injection driving motor conveyor belt assembly 7011 provided on the injection driving device bracket 7012 and a sample bearing support 7013 fixed on the injection driving motor conveyor belt assembly 7011. The sample tube 7014 is carried in the slot with an opening on one side of the sample bearing support 7013. The identification device 702 includes a sample information scanner 7021 fixed on the scanner bracket 7022. The monitoring device 703 includes a sample monitoring sensor 7031 fixed on the sample monitoring bearing bracket 7032.

[0094] It should be noted that the identity label of the sample tube 7014 can be used to record the sample information required for each sample detection, and specifically, two-dimensional codes or barcodes can be used. The sample information scanner 7021 scans the sample information and checks it with the background to avoid incorrect sample delivery.

[0095] Figure 6 The structural schematic diagram of the sampling mechanism provided for an embodiment shows a sampling mechanism.

[0096] See Figure 1 and Figure 6, the operating mechanism includes a sampling mechanism 5 disposed beside the first sample addition position b110; the sampling mechanism 5 includes a lateral driving device 503 installed on a vertical plate 501 and a vertical sample taking and placing device 502 that can be driven by the lateral driving device 503 to translate.

[0097] Specifically, the lateral driving device includes a lateral motor 5031 and a lateral belt assembly that can be driven by the lateral motor 5031. The vertical sample taking and placing device is connected to the lateral belt assembly and includes a shaker mixing device 5021, a preheating tank device 5022 behind the shaker mixing device 5021, a vertical driving motor 5024 on the left side of the shaker mixing device 5021, a liquid level detection device 5025 in front of the shaker mixing device 5021, a sampling needle anti-collision device 5023 between the shaker mixing device 5021 and the liquid level detection device 5025, and a sampling needle 5026 connected to the lower end of the liquid level detection device 5025.

[0098] It should be noted that the lateral driving device can drive the vertical sample taking and placing device to move horizontally above the first sample addition position b110, and then the vertical driving motor 5024 can drive the sampling needle 5026 to descend to the first sample addition position b110 for sample addition.

[0099] Figure 7 It is a schematic structural diagram of a puncture mechanism provided for an embodiment, showing a puncture mechanism that can perform puncture operations corresponding to the first incubation tank assembly b.

[0100] See Figure 1 and Figure 7 , the operating mechanism includes a first puncture mechanism b3 disposed beside the first puncture position b108; the first puncture mechanism b3 includes a puncture driving device installed on a first cross beam b307 and a first puncture needle b301 that can be driven by the puncture driving device to puncture downward.

[0101] Specifically, the first puncture needle b301 is fixed on a first puncture needle plate b302. The puncture driving device includes a first puncture slide rail b306 provided on the right side of the first cross beam b307. A first puncture needle connecting plate b303 is slidably disposed on the first puncture slide rail b306. The upper end of the first puncture needle connecting plate b303 is connected to a first puncture motor b305 through a first puncture motor fixing plate b304, and the lower end of the first puncture needle connecting plate b303 is fixed to the first puncture needle plate b302.

[0102] It should be noted that the first puncture motor b305 can drive the first puncture needle connecting plate b303 to move up and down on the first puncture slide rail b306, thereby driving the first puncture needle b301 fixed on the first puncture needle plate b302 to perform puncture at the first puncture position b108.

[0103] Figure 8Schematic diagram of the puncture mechanism provided for an embodiment, showing a puncture mechanism that can perform puncture operations corresponding to the second incubation tank assembly c.

[0104] See Figure 1 and Figure 8 , the operating mechanism includes a puncture mechanism 4 provided beside the second puncture position 2052; the puncture mechanism 4 includes a puncture driving device installed on the puncture mechanism body 405 and a puncture needle 401 that can be driven by the puncture driving device to puncture downward.

[0105] Specifically, the puncture needle 401 is fixed on the puncture needle fixing plate 402. The puncture driving device includes a linear guide pair 403 provided on the right side of the puncture mechanism body 405, an eccentric wheel 407 located on one side of the linear guide pair 403, a reset sensor 406 located on the left side of the eccentric wheel 407, and a puncture driving motor 404 located behind the eccentric wheel 407.

[0106] It should be noted that the puncture driving motor 404 can drive the puncture needle fixing plate 402 to move up and down on the linear guide pair 403, thereby driving the puncture needle 401 fixed on the puncture needle fixing plate 402 to perform puncture at the second puncture position 2052.

[0107] Figure 9 Schematic diagram of the luminescence detection mechanism provided for an embodiment, showing a luminescence detection mechanism that can perform luminescence detection corresponding to the first incubation tank assembly b.

[0108] See Figure 1 and Figure 9 , the operating mechanism includes a luminescence detection mechanism b2 provided above the first detection position b111; the luminescence detection mechanism b2 includes a slide rail b205 installed on one side of the support plate, a body driving motor b203 installed at the top of the support plate, and a single photon counter b201 with a light shielding structure connected to the body driving motor b203 through a single photon counting mounting block b202. The support plate includes a plate body b206 and a driving motor mounting block b204 and a base b207 provided at the upper and lower ends of the plate body b206.

[0109] It should be noted that the single photon counter 201 is used to detect the luminescence of the reactants in the chip cassette. Therefore, it is necessary not only to ensure that no stray light passes through the side wall of the chip cassette to irradiate the reactants, but also to set a light shielding structure at the part where the single photon counter 201 is docked with the chip cassette to block the stray light from entering the single photon counter 201.

[0110] Figure 10 Schematic diagram of the optical detection mechanism provided for an embodiment, showing an optical detection mechanism that can perform luminescence detection corresponding to the second incubation tank assembly c.

[0111] See Figure 1 andFigure 10 , the operating mechanism includes an optical detection mechanism 3 provided beside the second detection position; the optical detection mechanism 3 includes a light source device, a photoelectric conversion module, and a heat dissipation device for dissipating heat from the light source device, which are installed on the base.

[0112] Specifically, the base includes a scattering method optical module base 304 and a transmission method optical module base 305. The light source device includes a scattering laser light source 303 installed on the scattering method optical module base 304 and a transmission turbidimetry light source 301 installed on the transmission method optical module base 305. A transmission method receiving plate shield 306 is provided on one side of the transmission method optical module base 305, and a transmission method heat dissipation air duct 302 communicating with a heat dissipation fan 307 is provided below the transmission turbidimetry light source 301.

[0113] It should be noted that since the second detection positions (2054, 2055) communicating with the linear channel are provided on the side of the incubation tank 205, an optical path can be established to realize biochemical analysis of the sample. At the same time, the side wall of the chip cassette c204 is irradiated with light through the optical path, and after the light passes through the light-transmitting side wall, a photochemical reaction occurs with the sample, and then the light is detected by transmission turbidimetry or scattering turbidimetry through the transmission turbidimetry light source 301 and / or the scattering laser light source 303, so as to obtain the sample detection result.

[0114] Figure 11 It is a schematic structural diagram of a liquid suction and cleaning mechanism provided for an embodiment, showing a liquid suction and cleaning mechanism.

[0115] See Figure 1 and Figure 11 , the liquid suction and cleaning mechanism b4 includes a cleaning part and a driving part. The driving part drives the cleaning part to clean the inside of the first chip cassette. The driving part includes a liquid suction guide rail mounting plate b401 on which a liquid suction guide rail slider b405 is installed, and a guide rail connecting block b404 is installed on the liquid suction guide rail slider b405; a liquid suction driving motor b408 is installed at the top of the liquid suction guide rail mounting plate b401, and the liquid suction driving motor b408 is connected to the guide rail connecting block b404; a liquid suction pipe fixing block b406 is installed on the back of the liquid suction guide rail mounting plate b401.

[0116] The cleaning part includes a needle fixing seat b403 provided on one side of the guide rail connecting block b404. A guard coil fixing sheet metal b409 is provided at the upper end of the needle fixing seat b403. A cleaning needle b410 penetrates through the needle fixing seat b403. A cleaning needle distal positioning member base b412 provided with a cleaning needle distal positioning member b411 penetrates through the surface of the cleaning needle b410. One side of the cleaning needle distal positioning member base b412 is connected to a base support plate b402, and the base support plate b402 is slidably connected to the guide rail of the liquid suction guide rail slider b405.

[0117] Figure 12Schematic structural diagram of a liquid-electric unit provided for an embodiment, showing a liquid-electric unit.

[0118] See Figure 12 , the liquid-electric unit includes a liquid path valve 601, a liquid pump 602, a PCB board unit 603, a chassis cooling fan 604, a cooling fan air duct 605, a plunger pump 607, and a pressure detection device 608 mounted on a liquid-electric unit bracket 606.

[0119] It should be noted that the PCB board unit 603 and other components such as the liquid path valve 601 are respectively arranged on two opposite plate surfaces of the liquid-electric unit bracket 606, so as to achieve liquid-electric separation. The chassis cooling fan 604 and the cooling fan air duct 605 are connected and relatively arranged at the edge of the liquid-electric unit bracket 606, so as to achieve a good heat dissipation effect.

[0120] Figure 13 Schematic flow diagram of a detection method provided for an embodiment, showing a detection method.

[0121] See Figure 13 , the detection method includes:

[0122] S0. Set a first incubation tank module including a plurality of first operation positions along a straight line;

[0123] S1. Drive a first chip cartridge containing a disposable test reagent to move along the first operation position through the first incubation tank module for chemiluminescence detection;

[0124] S2. Set a second incubation tank module including a plurality of second operation positions along a straight line;

[0125] S3. Drive a second chip cartridge containing a disposable test reagent to move along the second operation position through the second incubation tank module for biochemical detection;

[0126] S4. Layout an operating mechanism beside the first operation position and the second operation position for corresponding operations.

[0127] The biochemical and immuno integrated analyzer provided by the present invention includes a first incubation tank module with a plurality of first operation positions arranged in a straight line. The first incubation tank module drives a first chip cartridge containing a disposable test reagent to move along the first operation positions for chemiluminescence detection. It also includes a second incubation tank module with a plurality of second operation positions arranged in a straight line. The second incubation tank module drives a second chip cartridge containing a disposable test reagent to move along the second operation positions for biochemical detection. Then, by arranging operation mechanisms beside the first operation positions and the second operation positions to perform corresponding operations, it effectively avoids the problem of the expiration date after opening the bottle and the operation of the "calibration" process, thereby making the detection process of the biochemical and immuno integrated analyzer with the above-mentioned incubation tank module simple and immediate and improving the detection accuracy.

[0128] See Figure 1-13 , in an embodiment, the corresponding action processes of the first incubation tank module and related operation mechanisms in the biochemical and immuno integrated analyzer are as follows:

[0129] First, place the sample tube 7014 into the sample bearing support (7013) of the sample injection mechanism 7, and at the same time select the first chip cartridge of the corresponding reagent package;

[0130] Second, insert the first chip cartridge into the first chip cartridge inlet b116 of the first incubation tank b100, and detect whether there is a first chip cartridge in the incubation tank through the first in-place sensor b112;

[0131] Third, after detecting the first chip cartridge, the sample information scanner 7021 scans and identifies the first chip cartridge;

[0132] Fourth, the first chip cartridge after being scanned and identified is transported to the first puncture position b108 by the first clamping mechanism 104;

[0133] Fifth, the first puncture needle b301 is activated to pierce the corresponding needle hole position on the first chip cartridge;

[0134] Sixth, the punctured first chip cartridge is transported to the first sample addition position b110 by the first clamping mechanism 104;

[0135] Seventh, the sample drive device 701 is activated to transport the sample tube 7014 to the end sampling mechanism 5 for sampling;

[0136] Eighth, the sampling mechanism 5 distributes the sample in the sample tube 7014 to the reaction cups of the corresponding first chip cartridge as needed through the sampling needle 5026, and at the same time distributes the reagent encapsulated in the first chip cartridge to the reaction cups of the first chip cartridge itself as needed. The movement trajectory of the sampling needle 5026 is in a straight line with the sample identification position, the needle cleaning pool position, and the second sample addition position 2051;

[0137] Ninth, the shaker mixing device 5021 on the sampling mechanism 5 performs corresponding mixing actions on the solution in the first chip cassette. After mixing, the first incubation tank b100 incubates the mixed solution for a period of time;

[0138] Tenth, after incubation, the first clamping mechanism 104 transports the first chip cassette to the first sample addition position b110, and the mixed solution receives the cleaning solution;

[0139] Eleventh, after the cleaning solution is added, the first clamping mechanism 104 sends the first chip cassette to the magnetic separation cleaning position b106;

[0140] Twelfth, the motion pump mechanism 13 operates, and through the linkage of the pump and in cooperation with the cleaning pool device f and the liquid suction cleaning mechanism 4, the waste liquid is pumped out, and the incubated solution is cleaned repeatedly for multiple times;

[0141] Thirteenth, after the cleaning is completed, the first clamping mechanism 104 sends the first chip cassette to the first sample addition position b110;

[0142] Fourteenth, the sampling needle 5026 drops the reagent into the reaction cup and incubates for a period of time;

[0143] Fifteenth, the first clamping mechanism 104 transfers the first chip cassette to the first detection position b111 for detection;

[0144] Sixteenth, after the detection is completed, the first clamping mechanism 104 transfers the first chip cassette to the first chip cassette inlet b116 of the first incubation tank b100.

[0145] See Figure 1-13 , in an embodiment, the corresponding action process of the first incubation tank module c and related operating mechanisms in the biochemical immune integrated analyzer is as follows:

[0146] First, insert the chip cassette c204 into the incubation tank 205, and detect the in-place situation of the chip cassette c204 through the second in-place sensor 206.

[0147] Second, drive the chip cassette c204 to move to the end of the incubation tank 205 according to the in-place instruction;

[0148] Third, scan the information of the chip cassette c204 and write it into the background;

[0149] Fourth, the sample driving device 701 starts to transport the sample tube 7014 to the end for the sampling mechanism 5 to sample;

[0150] Fifth, puncture the chip cassette c204;

[0151] Sixth, the sampling mechanism 5 takes the sample and distributes the encapsulated reagent to be mixed in the reaction cup of the chip cassette c204;

[0152] Seventh, shake the mixture well;

[0153] Eighth, incubate and detect the mixture;

[0154] Ninth, preheat the cleaning solution to clean the sampling mechanism 5.

[0155] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A biochemical and immunological integrated analyzer, characterized in that, Comprising: A first incubation tank module, including a plurality of first operation positions arranged in a straight line, for driving a first chip cartridge containing a disposable test reagent to move along the first operation positions for chemiluminescence detection; A second incubation tank module, including a plurality of second operation positions arranged in a straight line, for driving a second chip cartridge containing a disposable test reagent to move along the second operation positions for biochemical detection; An operating mechanism, arranged beside the first operation positions and the second operation positions for corresponding operations; The first incubation tank module includes a first incubation tank having a first linear channel inside; Above the first incubation tank, along the direction of the first linear channel, the first operation positions communicating with the first linear channel are arranged. The first operation positions include a first detection position, a first sample addition position, a first puncture position, and a magnetic separation and washing position, the distances from which to the entrance of the first chip cartridge increase successively; On one side of the first linear channel, a first driving mechanism for driving the first chip cartridge to pass through is arranged; The second incubation tank module includes a second incubation tank having a second linear channel inside; Above the second incubation tank, along the direction of the second linear channel, the second operation positions communicating with the second linear channel are arranged. The second operation positions include a second sample addition position, a second puncture position, a second detection position, and a second chip cartridge identification position, the distances from which to the entrance of the second chip cartridge increase successively; On one side of the second linear channel, a second driving mechanism for driving the second chip cartridge to pass through is arranged.

2. The biochemical and immunological integrated analyzer according to claim 1, characterized in that, The operating mechanism includes a sample addition mechanism arranged beside the first incubation tank module or the second incubation tank module; The sample addition mechanism includes a sample driving device for transporting a sample tube to move in a straight line, an identification device for identifying the identity label of the sample tube on one side of the sample driving device, and a monitoring device for detecting the presence or absence of the sample tube on one side of the sample driving device.

3. The biochemical and immunological integrated analyzer according to claim 1, characterized in that, The operating mechanism includes a sampling mechanism arranged above the first incubation tank module or the second incubation tank module; The sampling mechanism includes a lateral driving device installed on a vertical plate and a vertical sample taking and placing device that can be driven by the lateral driving device to translate.

4. The biochemical and immunological integrated analyzer according to claim 1, characterized in that, The operating mechanism includes a first puncture mechanism arranged above the first puncture position; The first puncture mechanism includes a first puncture driving device installed on a first cross beam and a first puncture needle that can be driven by the first puncture driving device to puncture downward.

5. The biochemical and immunological integrated analyzer according to claim 1, characterized in that, The operating mechanism includes a second puncture mechanism arranged above the second puncture position; The second puncture mechanism includes a second puncture driving device installed on a second cross beam and a second puncture needle that can be driven by the second puncture driving device to puncture downward.

6. A detection method applied to the biochemical and immunological integrated analyzer according to any one of claims 1-5, characterized in that, Comprising: A first incubation tank module including a plurality of first operation positions arranged in a straight line; Through the first incubation tank module, driving a first chip cartridge containing a disposable test reagent to move along the first operation positions for chemiluminescence detection; A second incubation tank module including a plurality of second operation positions arranged in a straight line; Through the second incubation tank module, driving a second chip cartridge containing a disposable test reagent to move along the second operation positions for biochemical detection; Layout an operating mechanism beside the first operating position and the second operating position to perform corresponding operations.

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