Portable plant photosynthetic rate detector and method thereof

Abstract

The present invention discloses a portable plant photosynthetic rate detector and a method thereof, and relates to the field of plant physiological function detection. The detector comprises a leaf (00), and is provided with a leaf chamber (10), a differential gas path unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50); the leaf (00) is placed in the leaf chamber (10), the leaf chamber (10) and the differential gas path unit (20) are connected, a sensor array (40) is arranged in a measuring chamber (23) of the differential gas path unit (20), and the sensor array (40) and the micro-control platform (50) are connected; the power supply (30) provides energy to the sensor array (40) and the micro-control platform (50). Compared with the prior art, the present invention has the following advantages and positive effects: ① the photosynthetic rate of plants under different CO2 concentrations, light intensities and temperature and humidity environmental conditions can be measured quickly, accurately and in real time; ② the structure is light, easy to carry and has a wide range of applications; ③ it supports outdoor use.

Description

Technical Field

[0001] The invention relates to the field of plant physiological function detection, and in particular to a portable plant photosynthetic rate detector and a method thereof. Background Art

[0002] Photosynthesis is an important characteristic of the growth and development of green plants. Measuring the photosynthetic rate of plants is an important indicator for judging the physiological characteristics of plants. The photosynthetic rate of plants can be used as a data to judge the suitable living environment of plants.

[0003] When plants perform photosynthesis, they absorb CO2 and water from the environment, generate carbohydrates and release oxygen. When plants perform photosynthesis, the light intensity and CO2 concentration in the environment are the main factors affecting the photosynthetic rate of plants. The temperature and humidity of the environment also affect the photosynthetic rate of plants.

[0004] Traditional methods for measuring the photosynthetic rate of plants are:

[0005] 1. Improved half-leaf method: This method requires the leaves to be separated from the plants before measurement. The operation is complicated, the experimental error is large, and it is impossible to measure the photosynthetic rate of plants in real time.

[0006] 2. Gas volume change method: This method can only measure some small plants and has limited scope of application.

[0007] 3. Infrared CO2 analysis method: This method is affected by the measurement site and cannot measure the photosynthetic rate of outdoor plants. Summary of the invention

[0008] The purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art and to provide a portable plant photosynthetic rate detector and method thereof, which can quickly, accurately and in real time measure the photosynthetic rate of plants under different CO2 concentrations, light intensities and temperature and humidity environmental conditions.

[0009] The object of the present invention is achieved in that:

[0010] The detector comprises a blade, and is provided with a blade chamber, a differential gas path unit, a power supply, a sensor array and a microcontroller platform;

[0011] The blade to be measured is placed in a blade chamber, the blade chamber is connected to a differential gas path unit, a sensor array is arranged in the measuring chamber of the differential gas path unit, and the sensor array is connected to a microcontroller platform;

[0012] The power supply provides energy to the sensor array and the microcontroller platform.

[0013] Compared with the prior art, the present invention has the following advantages and positive effects:

[0014] ① It can quickly, accurately and in real time measure the photosynthetic rate of plants under different CO2 concentrations, light intensities, temperature and humidity conditions;

[0015] ②Lightweight structure, easy to carry and widely applicable;

[0016] ③Support outdoor use. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the detector;

[0018] Figure 2 It is a schematic diagram of the structure of the leaf chamber.

[0019] In the figure:

[0020] 00—leaf;

[0021] 10 - leaf chamber,

[0022] 1A—upper blade chamber, 1B—lower blade chamber, 1C—screw,

[0023] 11—air inlet, 12—air outlet;

[0024] 20—Differential gas path unit,

[0025] 21—the first three-way solenoid valve, 22—the second three-way solenoid valve, 23—the measuring chamber, 24—the air pump;

[0026] 30—power supply;

[0027] 40—sensor array,

[0028] 41—CO2 sensor, 42—temperature and humidity sensor, 43—gas flow rate sensor,

[0029] 44—gas pressure sensor;

[0030] 50—Microcontroller platform,

[0031] 51—ADC sampling module, 52—pump speed control board, 53—wireless transmission module. DETAILED DESCRIPTION

[0032] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0033] 1. Detector

[0034] 1. Overall

[0035] like Figure 1 , the present invention includes a measured object - a blade 00, provided with a blade chamber 10, a differential gas path unit 20, a power supply 30, a sensor array 40 and a micro-control platform 50;

[0036] The blade 00 to be measured is placed in the blade chamber 10, the blade chamber 10 is connected to the differential gas path unit 20, a sensor array 40 is arranged in the measuring chamber 23 of the differential gas path unit 20, and the sensor array 40 is connected to the micro-control platform 50;

[0037] The power supply 30 provides energy to the sensor array 40 and the microcontroller platform 50 .

[0038] 2. Functional components

[0039] 0) Blade 00

[0040] 1) Leaf chamber 10

[0041] like Figure 2 The leaf chamber 10 includes an upper leaf chamber 1A, a lower leaf chamber 1B and a screw 1C connected in sequence. The upper leaf chamber 1A is provided with an air inlet 11 and an air outlet 12. A uniform grid is drawn on the glass sheet of the upper leaf chamber 1A to measure the leaf area when the leaves of the tested plant are too small to fill the entire leaf chamber. The area of ​​each small grid is 1mm 2 , the total area of ​​the grid is 900mm 2 The lower leaf chamber 1B is connected to the screw 1C, and the screw 1C is rotated to control the up and down movement of the lower leaf chamber 1A. Rubber pads are attached to the contact parts of the upper and lower leaf chambers 1A and 1B to optimize the air tightness of the leaf chamber 10 and protect the leaves of the tested plant 00 from being damaged when clamped.

[0042] The leaf chamber 10 is also provided with a control handle, and the opening and closing of the leaf chamber 10 is controlled by the control handle.

[0043] 2) Differential gas path unit 20

[0044] like Figure 1 , the differential air path unit 20 includes a first three-way solenoid valve 21, a second three-way solenoid valve 22, a measuring chamber 23 and an air pump 24;

[0045] The first three-way solenoid valve 21 and the second three-way solenoid valve 22 are connected to each other and are respectively connected to the air inlet 11 and the air outlet 12 of the leaf chamber 10. The second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 are connected in sequence.

[0046] A sensor array 40 is arranged in the measuring chamber 23 .

[0047] 3) Power supply 30

[0048] like Figure 1 The power source 30 is a large-capacity lithium-ion rechargeable battery with an input voltage of 12.6 VDC, an output voltage of 12.6-10.8 VDC, and a capacity of 6800 mAh.

[0049] 4) Sensor array 40

[0050] like Figure 1 The sensor array 40 includes a CO2 sensor 41, a temperature and humidity sensor 42, a gas flow rate sensor 43 and a gas pressure sensor 44.

[0051] (1) CO2 sensor 41

[0052] It is a universal sensor that measures the concentration of CO2.

[0053] (2) Temperature and humidity sensor 42

[0054] It is a universal sensor that measures temperature and humidity.

[0055] (3) Gas flow rate sensor 43

[0056] It is a universal sensor to measure gas flow rate.

[0057] (4) Gas pressure sensor 44

[0058] It is a universal sensor that measures gas pressure.

[0059] 5) Microcontroller platform 50

[0060] like Figure 1 The microcontroller platform 50 uses MSP430F149 as the CPU to process data and control other modules; it is embedded with an ADC sampling module 51, a pump speed control board 52 and a wireless transmission module 53.

[0061] The digital signal processed by the ADC sampling module 51 is received, the pump speed control board 52 is controlled to make the air pump 24 work, and the data is transmitted to the wireless transmission module 53 .

[0062] 3. Working mechanism

[0063] The CO2 sensor, gas flow rate sensor, atmospheric pressure sensor and temperature and humidity sensor in the sensor array are connected to the ADC sampling module to detect the CO2 concentration, air flow rate, atmospheric pressure and temperature and humidity in the leaf chamber and convert them into electrical signals. Then, through AD analog-to-digital conversion, the electrical signals are converted into digital signals recognizable by the microcontroller platform. The microcontroller platform analyzes and processes the digital signals to control the operation of the air pump and uses the Bluetooth wireless transmission module to send the data to the smart mobile device to generate a plant photosynthetic rate curve.

[0064] 2. Detection Method

[0065] ① Initialize the detection system:

[0066] Ⅰ. Turn on the power 30 to allow each module to work normally;

[0067] II. Connecting ports a and b of the first three-way solenoid valve 21 and ports e and d of the second three-way solenoid valve 22, so that the airflow flows in the air path in the direction of the dotted arrow, that is, the airflow flows in the direction in which the first three-way solenoid valve 21, the second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 are connected in sequence;

[0068] III. Make the sensor array 40 work normally, and transmit the detection data of each sensor to the micro-control platform 40. The micro-control platform 40 adjusts the operation of the air pump 24 according to the data detected by the atmospheric pressure sensor 43 and the gas flow rate sensor 42, and controls the gas flow rate in the differential gas path unit 20;

[0069] IV. The data measured by the CO2 sensor 41 is processed by Kalman algorithm filtering and converted by the ADC sampling module 51, and then uploaded to the smart mobile device through the Bluetooth wireless transmission module 53. If the measured CO2 concentration remains basically unchanged over time, the system initialization is completed;

[0070] ②After the system is initialized, it starts to detect the photosynthetic rate of the plant under test, including the following steps:

[0071] ⅰ. Press the button of the opening and closing control handle of the leaf chamber 10 to clamp the leaf 00. If the contact area of ​​the leaf 00 is too small to fill the entire leaf chamber 10, count the number of small grids on the glass sheet of the upper leaf chamber 1A occupied by the leaves in the leaf chamber 10. The area of ​​each small grid is 1mm 2 , estimate the area of ​​blade 00. If blade 00 can fill the entire leaf chamber 10, the area of ​​blade 00 can be regarded as the area of ​​the entire leaf chamber 1600mm 2 ;

[0072] ii. Ports a and c of the first three-way solenoid valve 21 are connected, port b is closed, ports d and f of the second three-way solenoid valve 22 are connected, and port e is closed, so that the airflow flows in the air path in the direction of the solid arrow, that is, the airflow flows in the direction in which the first three-way solenoid valve 21, the leaf chamber 10, the second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 are connected in sequence;

[0073] ⅲ. Keep measuring for a while, turn on the Bluetooth function of the smart mobile device, receive the data sent by the detector via Bluetooth, and collect data for a period of time to generate a plant photosynthetic rate curve.

Claims

1. A portable plant photosynthetic rate detector, comprising a leaf (00), characterized in that: A leaf chamber (10), a differential gas path unit (20), a power source (30), a sensor array (40) and a micro-control platform (50) are provided; The blade (00) is placed in a blade chamber (10), the blade chamber (10) is connected to a differential gas path unit (20), a sensor array (40) is arranged in a measuring chamber (23) of the differential gas path unit (20), and the sensor array (40) is connected to a micro-control platform (50); The power supply (30) provides energy to the sensor array (40) and the microcontroller platform (50); The leaf chamber (10) comprises an upper leaf chamber (1A), a lower leaf chamber (1B) and a screw (1C). The upper leaf chamber (1A) is provided with an air inlet (11) and an air outlet (12). A uniform grid is drawn on the glass sheet of the upper leaf chamber (1A) for measuring the leaf area of ​​the plant under test when the leaf is too small to fill the entire leaf chamber. The area of ​​each small grid is 1 mm2, and the total area of ​​the grid is 900 mm2. The lower leaf chamber (1B) is connected to the screw (1C). The lower leaf chamber (1B) is controlled to move up and down by rotating the screw (1C). A rubber pad is attached to the contact part between the upper and lower leaf chambers (1A, 1B) to optimize the air tightness of the leaf chamber and protect the leaf (00) of the plant under test from being damaged when being clamped. The leaf chamber (10) is also provided with a control handle, and the opening and closing of the leaf chamber (10) is controlled by the control handle; The differential air path unit (20) comprises a first three-way solenoid valve (21), a second three-way solenoid valve (22), a measuring chamber (23) and an air pump (24); A first three-way solenoid valve (21) and a second three-way solenoid valve (22) are connected to each other and are respectively connected to the air inlet (11) and the air outlet (12) of the leaf chamber (10); the second three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) are connected in sequence; A sensor array (40) is arranged in the measurement chamber (23); The sensor array (40) comprises a CO2 sensor (41), a temperature and humidity sensor (42), a gas flow rate sensor (43) and a gas pressure sensor (44); The microcontroller platform (50) uses MSP430F149 as a CPU to process data and control other modules; it is embedded with an ADC sampling module (51), a pump speed control board (52) and a wireless transmission module (53). Receiving a digital signal processed by an ADC sampling module (51), controlling a pump speed control board (52) to operate the air pump (24), and transmitting the data to a wireless transmission module (53); The detection method of the portable plant photosynthetic rate detector: ① Initialize the detection system: Ⅰ. Turn on the power and let each module work normally; II. Connecting ports a and b of the first three-way solenoid valve (21) and ports e and d of the second three-way solenoid valve (22) so that the airflow flows in the direction in which the first three-way solenoid valve (21), the second three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) are connected in sequence; III. The sensor array (40) operates normally and transmits the detection data of each sensor to the micro-control platform (50). The micro-control platform (50) regulates the operation of the air pump (24) and controls the gas flow rate in the differential gas path unit (20) according to the data detected by the gas flow rate sensor (43) and the atmospheric pressure sensor (44); IV. The data measured by the CO2 sensor (41) is processed by Kalman algorithm filtering and converted by the ADC sampling module (51) to digital-to-analog conversion, and then uploaded to the smart mobile device via the Bluetooth wireless transmission module (53). If the measured CO2 concentration remains basically unchanged over time, the system initialization is completed; ②After the system is initialized, it starts to detect the photosynthetic rate of the plant under test, including the following steps: i. Press the button of the opening and closing control handle of the leaf chamber (10) to clamp the leaf (00). If the contact area of ​​the leaf (00) is too small to fill the entire leaf chamber (10), count the number of small grids on the upper leaf chamber glass sheet occupied by the leaf in the leaf chamber (10). The area of ​​each small grid is 1 mm2. Estimate the area of ​​the leaf. If the leaf fills the entire leaf chamber (10), the area of ​​the leaf (00) is regarded as the area of ​​the entire leaf chamber 1600 mm2. ii. connecting ports a and c of the first three-way solenoid valve (21) and closing port b, connecting ports d and f of the second three-way solenoid valve (22) and closing port e, so that the air flow in the air path flows in the direction in which the first three-way solenoid valve (21), the leaf chamber (10), the second three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) are connected in sequence; ⅲ. Keep measuring for a while, turn on the Bluetooth function of the smart mobile device, receive the data sent by the detector via Bluetooth, and generate a plant photosynthetic rate curve.

2. The portable plant photosynthetic rate detector according to claim 1, characterized in that: The power source (30) is a large-capacity lithium-ion rechargeable battery with an input voltage of 12.6 VDC, an output voltage of 12.6-10.8 VDC, and a capacity of 6800 mAh.

Images