A constant temperature fully automated cell slice preparation integrated device and preparation method

Abstract

The present invention discloses a constant-temperature fully-automated integrated cell preparation device, which comprises: a rotating sedimentation plate, which comprises a sedimentation plate seat and a turntable body connected to the sedimentation plate body by a fixed shaft; a slide adding mechanism arranged at a slide entrance position close to the rotating sedimentation plate, which pushes the slide to be loaded from the slide entrance position into the rotating sedimentation plate; a cleaning and loading integrated device, wherein the cleaning and liquid adding integrated device is arranged beside the rotating sedimentation plate, the cleaning and liquid adding integrated device comprises a cleaning liquid component and a loading component, and the cleaning liquid component and the loading component respectively add liquid into two different sedimentation cups simultaneously with the intermittent rotation of the turntable body; and a slide discharging mechanism arranged at a slide exit position close to the rotating sedimentation plate, wherein the slide discharging mechanism moves the loaded slide out from the slide exit position.

Description

Technical Field

[0001] The present invention relates to the technical field of instruments for pathological cells, diagnostics, and pathological biomolecular diagnostics, and in particular to a cell sheet device, and in particular to a fully automatic liquid-based thin layer cell sheet making device and a sheet making method. Background Art

[0002] In medical testing and biological research, it is often necessary to prepare and stain cell samples for better observation. Traditional preparation and staining processes are all done manually. However, manual smears contain a large amount of cell accumulation and interfering factors such as red blood cells, mucus, and impurities, which affect the correct diagnosis. There will be a false negative rate of 2% to 50%, and it is easy to cause eye fatigue for the diagnostic staff. It is time-consuming, labor-intensive, and inefficient. In recent years, the application of liquid-based cytology technology has made new breakthroughs in cell preparation and staining technology. Liquid-based cytology technology is a method of preserving exfoliated cells in cell preservation fluid. It can effectively remove interfering diagnostic factors such as red blood cells, mucus, and impurities, and disperse and dissociate cell clusters, significantly improving the quality of pathological cytology preparation. The cell structure and background are clear, which is conducive to differential diagnosis and significantly reduces the false negative rate.

[0003] Through the search of literature and patents, patents and documents related to cell preparation, such as the Chinese patent document with publication number CN106018033A and publication date October 12, 2016, entitled "Fully Automatic Liquid-Based Thin-Layer Cell Slide Preparation and Staining Device," disclose a fully automatic liquid-based thin-layer cell slide preparation and staining device. In the technical solution disclosed in this patent document, the device can fully automatically implement the mechanisms of setting out, pipetting, slide preparation, and staining. Although it can automatically prepare slides to a certain extent and partially replace manual operations, the slide preparation and staining device will result in different staining depths at different temperatures and lacks effective quality control. At the same time, it can only achieve setting out and staining, but cannot achieve automation of the entire process such as cleaning and collecting slides. Therefore, manual participation is still required, which increases labor costs and production time, which is not conducive to reducing the cost of the entire process. Summary of the Invention

[0004] In view of the defects in the prior art, one of the objectives of the present invention is to provide a constant temperature fully automated cell preparation integrated device and a method for using the same.

[0005] In view of this, the present invention proposes a constant temperature fully automated cell preparation integrated device, which comprises:

[0006] A rotary sedimentation plate, comprising a sedimentation plate base and a turntable body connected to the sedimentation plate body via a fixed shaft, wherein a temperature control assembly is provided at the bottom of the sedimentation plate base, and a slide inlet, a slide outlet, and a cleaning and sample loading inlet are sequentially arranged on the sidewall of the sedimentation plate base; at least two sedimentation cup accommodating grooves are evenly arranged along the circumferential edge of the turntable body to accommodate the sedimentation cups; the turntable body intermittently rotates around the fixed shaft, and at least four slide slots are formed between the turntable body and the rotary sedimentation plate base, wherein slides to be loaded or loaded are adaptively received in the slide slots;

[0007] A slide loading mechanism, which is located near the slide entrance of the rotary sedimentation tray and pushes the slide to be loaded into the rotary sedimentation tray from the slide entrance;

[0008] The cleaning and adding integrated device is arranged beside the rotating sedimentation plate, and includes a cleaning liquid component and a adding component. The cleaning liquid component and the adding component respectively add liquid into two different sedimentation cups simultaneously with the intermittent rotation of the turntable body;

[0009] The slide discharging mechanism is arranged at a slide exit position close to the rotary sedimentation plate, and the slide discharging mechanism moves the sample-loaded slide out from the slide exit position.

[0010] Preferably, the outer wall and bottom of the sedimentation plate seat are provided with an insulation layer, the inner layer of the insulation layer close to the turntable body is provided with insulation cotton, and the outer layer away from the turntable body is provided with a metal cladding.

[0011] Preferably, the temperature control assembly includes a temperature control element and a heating device connected to the temperature control element, the heating device is arranged at a position of the sedimentation tray seat corresponding to the sedimentation cup accommodating groove, and the temperature control element includes a resistance regulator.

[0012] Preferably, the temperature control component controls the temperature of the sedimentation cup in the sedimentation cup accommodating tank to be 20-60°C.

[0013] Preferably, the slide adding mechanism includes a slide box with an opening and a pushing device, and the pushing device pushes the slides stored in the slide box through the opening to the slide entrance position, and then the slides enter the slide slots of the rotating sedimentation tray from the slide entrance.

[0014] Preferably, the cleaning and sample loading integrated device further comprises a robotic arm, one end of which is connected to the Z-axis adjustment mechanism, and the other end of which is connected to the fluid of the cleaning liquid assembly and the sample loading assembly;

[0015] The Z-direction adjustment mechanism reciprocates in the vertical direction to adjust the relative height of the robotic arm with respect to the turntable body.

[0016] Preferably, the robotic arm has an arc-shaped portion.

[0017] Preferably, the sheet discharging mechanism includes a sheet discharging fork and a driving mechanism connected to the sheet discharging fork.

[0018] Preferably, the sedimentation cup accommodating groove and the slide card slot are arranged at intervals. When the turntable body rotates intermittently, the space between the slide inlet, the slide outlet and the slide card slot at the cleaning and sample addition inlet is connected to the sedimentation cup accommodating groove.

[0019] Accordingly, the present invention also proposes a method for using the above-mentioned constant temperature fully automated cell preparation integrated device, comprising the following steps:

[0020] Step S1: The slide to be loaded enters the rotating sedimentation tray through the slide inlet and rotates intermittently along with the turntable body;

[0021] Step S2: The cleaning liquid component and the sample loading component in the cleaning and loading integrated device add liquid to two different sedimentation cups respectively along with the intermittent rotation of the turntable body. At this time, the sample loading component adds sample to the glass slide to be loaded through the sedimentation cup, while the cleaning component cleans the sedimentation cup that has been loaded with sample, and the loaded glass slide is removed from the slide outlet.

[0022] Compared with the prior art, the constant temperature fully automated cell preparation integrated device and its use method of the present invention have the following advantages and beneficial effects:

[0023] ⑴Under different temperatures, the depth of dye coloring varies, resulting in different film production effects. By adopting a temperature control system, a stable external environment can be provided for the dyeing reaction under different seasonal and regional conditions, thereby achieving stable film production effects.

[0024] ⑵ By adopting an original sedimentation cup, equipped with a cleaning mechanism and cleaning reagents, the sedimentation cup can be used multiple times, avoiding the impact of repeated filling of the sedimentation cup on efficiency, saving consumables and reducing costs.

[0025] ⑶ By adopting integrated reagent needles and integrated cleaning needles that work together, the sedimentation cup can be cleaned at the same time as Papanicolaou staining, which optimizes the mechanical structure, reduces sample waiting time, and greatly improves production efficiency.

[0026] ⑷ Through the large-capacity slide box and automatic slide pushing structure, the number of manual interventions can be reduced, the number of times consumables are loaded is greatly reduced, and the automation level of the entire production process is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Other features, objects and advantages of the present invention will become more apparent upon reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0028] Figure 1 The constant temperature fully automated cell preparation integrated device of the present invention;

[0029] Figure 2 for Figure 1 Schematic diagram of the decomposed structure of the rotating sedimentation plate in the constant temperature fully automated cell preparation integrated device.

[0030] Figure 3 The structure of the rotating sedimentation plate in some embodiments of the constant temperature fully automated cell preparation integrated device of the present invention is schematically shown from another perspective;

[0031] Figure 4 The diagram illustrates the sample loading operation on the rotary sedimentation plate in the constant temperature fully automated integrated cell preparation device of the present invention;

[0032] Figure 5 The figure illustrates the cleaning operation of the rotary sedimentation plate in the constant temperature fully automated cell preparation integrated device of the present invention;

[0033] Figure 6 It is the fork structure in the constant temperature fully automated cell preparation integrated device of the present invention;

[0034] Figure 7 The diagram illustrates the slide pushing mechanism in the constant temperature fully automated cell slide preparation integrated device of the present invention;

[0035] Figure 8 The figure illustrates the operation of the shift fork for discharging the slices by the slice pushing device in the constant temperature fully automated integrated cell slice preparation device of the present invention.

[0036] The reference numerals are as follows:

[0037] DETAILED DESCRIPTION

[0038] The present invention will be described in detail below with reference to specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those skilled in the art, several changes and improvements can be made without departing from the scope of the present invention. These all fall within the scope of protection of the present invention.

[0039] Example 1

[0040] Figure 1 This is the constant temperature fully automated cell preparation integrated device described in the present invention.

[0041] like Figure 1 shown, and when necessary, combined Figures 2 to 8 The fully automatic liquid-based thin-layer cell slide preparation device includes a rotating sedimentation tray 12, a slide storage and push mechanism 25, a sample injection needle 01, a pipetting mechanism 26, and a cleaning assembly 29. The sedimentation tray 12 includes a constant-temperature turntable 17 with a plurality of spaced slide slots 23 arranged along its periphery, a heat-insulating housing 16, and a sedimentation preparation assembly 30. The slide storage and push mechanism 25 includes a slide cassette 02, a slide entry fork 08, and a slide exit fork 13. The pipetting mechanism 26 includes a reagent arm Z-axis 10, a transverse reagent arm R-axis 09, and a reagent needle assembly 27 located at the end of the transverse reagent arm R-axis 09. The reagent needle assembly 27 is arc-shaped and positioned directly above the sedimentation cup 05.

[0042] Furthermore, a temperature control system is integrated within the thermostatic turntable 17, and the heat-insulating outer shell 16 encloses the periphery and lower portion of the sedimentation tray 12. The sedimentation preparation assembly 30 includes a slide holder 23, a lifting mechanism 07, and a sedimentation cup 05. The lifting mechanism 07 controls the vertical movement of the sedimentation cup 05 along the central axis of the thermostatic turntable 17.

[0043] The lifting mechanism 07 controls the sedimentation cup 05 to lift up, and the fork motor 18 drives the slide fork 08 to push the slide in the slide box 02 into the slide slot 23 from the slide entrance 24. The lifting mechanism 07 controls the sedimentation cup 05 to descend, so that the bottom of the sedimentation cup 05 is tightly connected with the slide.

[0044] The reagent needle assembly 27 includes a vertically arranged arc-shaped bracket 28, an integrated reagent needle 04, and an integrated cleaning needle 03. The integrated reagent needle 04 and the integrated cleaning needle 03 are connected to the external container via an internal suction pipe. The lifting drive motor 19 drives the reagent arm Z axis 10 up and down to complete the reagent filling and waste liquid extraction. The cleaning assembly 29 includes a cleaning plate and a cleaning plate pushing mechanism 11.

[0045] Each time the constant temperature turntable 17 completes a Pap staining or sedimentation cup cleaning, the constant temperature turntable 17 rotates at least once to transfer the next sedimentation cup 05 to be operated to the bottom of the reagent needle assembly 27.

[0046] The integrated reagent needle 04 and the integrated cleaning needle 03 work together. When the sample in one sedimentation cup is subjected to Pap staining, the other sedimentation cup in a washable state can be cleaned.

[0047] More specifically, the temperature control system includes a heating belt 14 and a temperature control assembly 15. The temperature can be controlled to be within the range of 20°C to 45°C, and the outer heat-insulating shell 16 provides a stable external environment for the dyeing reaction.

[0048] More specifically, the slide box 02 loaded with slides fastens the slide box buckle 22 and inserts it into the slide box slot 21 to complete the fixation. The lifting mechanism 07 controls the sedimentation cup 05 to rise, and the slide fork 08 pushes the slide out, and through the rotation of the rotary sedimentation plate 12, it enters the corresponding slide slot 23 from the slide entrance 24. The lifting mechanism 07 lowers the sedimentation cup 05, and the bottom of the sedimentation cup 05 is closed with the slide. The sample injection needle 01 injects the sample into the sedimentation cup 05, and the constant temperature turntable rotates the sedimentation cup to the bottom of the integrated reagent needle. The lifting drive motor 19 drives the reagent arm Z axis 10 to descend and perform the Pap staining process. After the Pap staining is completed, the slide slot rotates to the slide discharge position, the slide discharge fork 13 first descends, and enters the constant temperature turntable from the slide outlet 32. The slide discharge fork 13 rises to the bottom of the slide, and the slide discharge fork 13 withdraws to pull the slide out. The slide card slot rotates to the cleaning position, the lifting mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism 11 to push the cleaning plate from the cleaning plate inlet 33 into the slide card slot 23, the lifting mechanism controls the sedimentation cup 05 to descend, the bottom of the sedimentation cup 05 is closed with the cleaning plate 31 to form a closed structure, the integrated cleaning needle 03 passes the cleaning reagent into the sedimentation cup 05, after the cleaning is completed, the integrated cleaning needle 03 extracts the waste liquid, the lifting mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism to pull out the cleaning plate, and the lifting mechanism controls the sedimentation cup to descend.

[0049] Reagents and stains are transferred via an integrated reagent needle, sedimentation cup cleaning fluid is transferred via an integrated cleaning needle, and the slides include cationic high-adsorption slides.

[0050] A fully automated liquid-based thin-layer cell slide preparation device includes a rotating sedimentation tray 12, a slide storage and push mechanism 25, a sample injection needle 01, a pipetting mechanism 26, and a cleaning assembly 29. The sedimentation tray 12 comprises a thermostatic turntable 17 with a plurality of spaced slide slots 23 arranged along its periphery, a heat-insulating housing 16, and a sedimentation preparation assembly 30. The slide storage and push mechanism 25 includes a slide cassette 02, a slide entry fork 08, and a slide exit fork 13. The pipetting mechanism 26 includes a reagent arm Z-axis 10, a transverse reagent arm R-axis 09, and a reagent needle assembly 27 located at the end of the transverse reagent arm R-axis 09. The reagent needle assembly 27 is arc-shaped and positioned directly above the sedimentation cup 05.

[0051] Furthermore, a temperature control system is integrated within the thermostatic turntable 17, and the heat-insulating outer shell 16 encloses the periphery and lower portion of the sedimentation tray 12. The sedimentation preparation assembly 30 includes a slide holder 23, a lifting mechanism 07, and a sedimentation cup 05. The lifting mechanism 07 controls the vertical movement of the sedimentation cup 05 along the central axis of the thermostatic turntable 17.

[0052] The lifting mechanism 07 controls the sedimentation cup 05 to lift up, and the fork motor 18 drives the slide fork 08 to push the slide in the slide box 02 into the slide slot 23 from the slide entrance 24. The lifting mechanism 07 controls the sedimentation cup 05 to descend, so that the bottom of the sedimentation cup 05 is tightly connected with the slide.

[0053] The reagent needle assembly 27 includes a vertically arranged arc-shaped bracket 28, an integrated reagent needle 04, and an integrated cleaning needle 03. The integrated reagent needle 04 and the integrated cleaning needle 03 are connected to the external container via an internal suction pipe. The lifting drive motor 19 drives the reagent arm Z axis 10 up and down to complete the reagent filling and waste liquid extraction. The cleaning assembly 29 includes a cleaning plate and a cleaning plate pushing mechanism 11.

[0054] Each time the constant temperature turntable 17 completes a Pap staining or sedimentation cup cleaning, the constant temperature turntable 17 rotates at least once to transfer the next sedimentation cup 05 to be operated to the bottom of the reagent needle assembly 27.

[0055] The integrated reagent needle 04 and the integrated cleaning needle 03 work together. When the sample in one sedimentation cup is subjected to Pap staining, the other sedimentation cup in a washable state can be cleaned.

[0056] More specifically, the temperature control system includes a heating belt 14 and a temperature control assembly 15. The temperature can be controlled to be within the range of 20°C to 45°C, and the outer heat-insulating shell 16 provides a stable external environment for the dyeing reaction.

[0057] More specifically, the slide box 02 loaded with slides fastens the slide box buckle 22 and inserts it into the slide box slot 21 to complete the fixation. The lifting mechanism 07 controls the sedimentation cup 05 to rise, and the slide fork 08 pushes the slide out, and through the rotation of the rotary sedimentation plate 12, it enters the corresponding slide slot 23 from the slide entrance 24. The lifting mechanism 07 lowers the sedimentation cup 05, and the bottom of the sedimentation cup 05 is closed with the slide. The sample injection needle 01 injects the sample into the sedimentation cup 05, and the constant temperature turntable rotates the sedimentation cup to the bottom of the integrated reagent needle. The lifting drive motor 19 drives the reagent arm Z axis 10 to descend and perform the Pap staining process. After the Pap staining is completed, the slide slot rotates to the slide discharge position, the slide discharge fork 13 first descends, and enters the constant temperature turntable from the slide outlet 32. The slide discharge fork 13 rises to the bottom of the slide, and the slide discharge fork 13 withdraws to pull the slide out. The slide card slot rotates to the cleaning position, the lifting mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism 11 to push the cleaning plate from the cleaning plate inlet 33 into the slide card slot 23, the lifting mechanism controls the sedimentation cup 05 to descend, the bottom of the sedimentation cup 05 is closed with the cleaning plate 31 to form a closed structure, the integrated cleaning needle 03 passes the cleaning reagent into the sedimentation cup 05, after the cleaning is completed, the integrated cleaning needle 03 extracts the waste liquid, the lifting mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism to pull out the cleaning plate, and the lifting mechanism controls the sedimentation cup to descend.

[0058] In the above process, the operation flow of the constant temperature fully automated cell preparation device of the present invention is as follows:

[0059] The glass slide is automatically loaded onto the machine and sealed with the sedimentation cup;

[0060] The treated sample is added to the sedimentation cup and allowed to settle at a constant temperature;

[0061] The sedimented cells were stained with Papanicolaou stain;

[0062] Transfer the Papanicolaou-stained slides out of the slide slot and clean the sedimentation cup;

[0063] The treated sample is added to the sedimentation cup through the sample needle;

[0064] Among them, the temperature of constant temperature static sedimentation is controlled between 20℃-60℃.

[0065] When performing Papanicolaou staining, the operation steps are as follows: let the sample settle for 3-15 minutes, remove the supernatant, add 0.5-2 ml of anhydrous ethanol or isopropanol, and remove it after 8-12 seconds; then add 0.5-2 ml of pH 7.7 tris buffer, and remove it after 3-8 seconds; add 0.5-2 ml of hematoxylin stain, and remove it after 1-5 minutes; add pH 7.7 Add 0.5-2 ml of tris buffer and aspirate it after 0.5-1 minute; add 0.5-2 ml of anhydrous ethanol or isopropanol and aspirate it after 3-8 seconds; add 0.5-2 ml of anhydrous ethanol or isopropanol and aspirate it after 3-8 seconds; add 0.5-2 ml of EA-OG mixed dye and aspirate it after 1-5 minutes; add 0.5-2 ml of anhydrous ethanol or isopropanol and aspirate it after 5-10 seconds; add 0.5-2 ml of anhydrous ethanol or isopropanol and aspirate it after 5-10 seconds; after the slide fork transfers the slide out of the slide slot, move the cleaning plate to the slide slot and clean the sedimentation cup.

[0066] Example 2

[0067] The constant temperature fully automated integrated cell preparation device in this embodiment also includes a sealing device, which is used to seal the glass slide after the glass slide is transferred out of the glass slide slot. The sealing device includes a frame, a fork, a glue adding arm, a sealing glue reagent needle, a curing cup, a curing box, an ultraviolet lamp, a first control motor 7 and a second control motor.

[0068] In which, the rack is provided with a sample slide rail, the fork is provided at one end of the sample slide rail and can slide in the sample slide rail, a curing box and an ultraviolet lamp are provided above the sample slide rail in sequence from one side where the fork is provided to the other side, a curing cup is provided on the curing box, a glue adding arm is provided above the curing cup, and a sealing glue reagent needle is provided on the glue adding arm; the first control motor and the second control motor are provided on the rack, the first control motor is connected to the glue adding arm and can drive the glue adding arm to move in the vertical direction, and the second control motor is connected to the glue adding arm and can drive the glue adding arm to move in the horizontal direction; the slide is placed in the sample slide rail and can slide in the sample slide rail under the action of the fork.

[0069] In addition, the first control motor is connected to the gluing arm through a gear or belt transmission mechanism, and can drive the gluing arm to rotate in the horizontal direction; the second control motor is connected to the gluing arm through a gear rack mechanism or a worm gear mechanism, and can drive the gluing arm to move up and down.

[0070] The curing box has a receiving slot at the bottom, which can accommodate a glass slide. A through-hole is located in the center of the curing box, which accommodates the curing cup and communicates with the receiving slot. A guide spring post is located at the bottom of the curing box, and the bottom of the curing cup is hollowed out. Limiting slots are provided around the curing cup's circumference, and protruding blocks are provided around the curing cup, which mate with the limiting slots.

[0071] In addition, the width of the receiving groove at the bottom of the curing box matches the size of the sample slide. When the glue-adding arm presses the curing cup, the receiving groove at the bottom of the curing box clamps the sample slide to prevent the sample slide from moving. The size of the through-hole matches the size of the curing cup. The blocks arranged on the circumference of the curing cup match and connect with the limiting slots on the curing box, thereby limiting the curing cup to be firmly installed on the curing box without moving or rotating, thereby not affecting the subsequent glue-dropping process and keeping the glue-dropping position fixed. The guide spring column at the bottom of the curing box is in an energy storage state when the glue-adding arm presses the curing cup against the surface of the sample slide. When the glue-adding arm is lifted, the guide spring column releases the stored energy and automatically lifts the curing cup, thereby separating the curing cup from the sample slide. The bottom of the curing cup is hollowed out into a circle, so that the sealing glue forms a circular solidified layer at a fixed position on the sample slide.

[0072] Preferably, in some embodiments, a lampshade may be provided outside the UV lamp, and the lampshade is located above the sample slide, and the sample slide after the glue is dispensed is cured by the UV lamp in the lampshade.

[0073] The glue-dispensing arm is equipped with a sealing glue container, an injection system, a suction system, and a waste glue container. The reagent needle includes a glue-dispensing needle and a glue-sucking needle. The sealing glue container is connected to the injection system, which is connected to the glue-dispensing needle. The glue-sucking needle is connected to the suction system, which is connected to the waste glue container. There are one or more glue-sucking needles, evenly arranged around the glue-dispensing needle. The injection system is equipped with a metering device.

[0074] The injection system can drip the sealing glue in the sealing glue holding device onto the surface of the sample slide through the glue adding needle. The volume of glue injected by the injection system is measured by the metering device, and the volume of the glue dripping is controlled by the metering device; the suction system can suck the excess sealing glue into the waste glue holding device through the glue suction needle. In one embodiment, four glue suction needles are evenly arranged around the glue adding needle, namely, a first glue suction needle, a second glue suction needle, a third glue suction needle and a fourth glue suction needle.

[0075] Example 3

[0076] In this embodiment, the constant temperature fully automated integrated cell slide preparation device further includes a multi-well plate filter device, which is used to perform positive pressure blowing on the slide.

[0077] The porous plate filtration device includes a porous plate, a clamp, a movable assembly, a guide block, an air positive pressure block, and a frame; the movable assembly, guide block, and air positive pressure block are all mounted on the frame; the clamp is connected to the movable assembly and can move under the action of the movable assembly; the clamp is capable of grasping the porous plate; the porous plate is mounted on the guide block, which is located below the air positive pressure block. The movable assembly includes an X-axis slide and a Y-axis slide; the Y-axis slide is mounted on the X-axis slide and can move on the X-axis slide; the clamp is mounted on the Y-axis slide and can move on the Y-axis slide. A stacking assembly is also included, which includes multiple layers of placement positions, each of which can accommodate the porous plate.

[0078] The guide block is movably connected to the frame, and the guide block can move horizontally and rotate below the air positive pressure block. The air positive pressure block is movably connected to the frame, and the air positive pressure block can move horizontally and / or rotate above the guide block; the air positive pressure block can be connected to external air through a pipeline.

[0079] The porous plate includes a grid, a filter membrane, and a filtrate tray. The filter membrane is connected to the bottom of the grid, and the filtrate tray is detachably connected below the filter membrane. The edges of the porous plate are turned outward to form a clamping position. One or more clips are provided at the bottom of the edge of the porous plate, and one or more slots are provided on the clamping jaws. When the clamping jaws are clamped in the clamping position of the porous plate, the clips and slots are matched and connected in a one-to-one correspondence; a cut corner is provided on one edge of the porous plate to form a placement mark. There are multiple grids, and the multiple grids are arranged in multiple rows and columns; the grids are arch-shaped, T-shaped, or triangular. The filtration pore diameter of the filter membrane is 1-50 microns.

[0080] Preferably, in some embodiments, the porous plate includes 24 gate arch-shaped holes, arranged in 6 rows and 4 columns, the filter membrane uses a screen, the screen is welded to the bottom of the hole by a heat welding process, the diameter of the arc portion of the gate arch hole is φ4-50 mm, the width of the straight edge of the gate arch hole is 4-50 mm, the depth of the gate arch hole is 5-40 mm, the liquid filling volume of the porous plate is 20-3000 microliters, and the material of the porous plate is PES (polyethersulfone resin), PE (polyethylene) or PET (polyethylene terephthalate).

[0081] It should be noted that the prior art within the scope of protection of the present invention is not limited to the embodiments given in this application document. All prior art that does not contradict the solutions of the present invention, including but not limited to prior patent documents, prior publications, prior public uses, etc., can be included in the scope of protection of the present invention.

[0082] In addition, the combination of the various technical features in this case is not limited to the combination described in the claims of this case or the combination described in the specific embodiments. All technical features recorded in this case can be freely combined or combined in any way unless there is a contradiction between them.

[0083] It should also be noted that the embodiments listed above are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and similar changes or modifications made therefrom that can be directly derived from or easily conceived by those skilled in the art based on the disclosure of the present invention are intended to fall within the scope of protection of the present invention.

[0084] The above describes specific embodiments of the present invention. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art may make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. The embodiments of this application and the features in the embodiments may be combined with each other in any manner unless there is a conflict.

Claims

1. A constant temperature fully automated cell preparation integrated device, characterized in that: It includes: A rotary sedimentation plate, comprising a sedimentation plate base and a turntable body connected to the sedimentation plate body via a fixed shaft, wherein a temperature control assembly is provided at the bottom of the sedimentation plate base, and a slide inlet, a slide outlet, and a cleaning and sample loading inlet are sequentially arranged on the sidewall of the sedimentation plate base; at least two sedimentation cup accommodating grooves are evenly arranged along the circumferential edge of the turntable body to accommodate the sedimentation cups; the turntable body intermittently rotates around the fixed shaft, and at least four slide slots are formed between the turntable body and the rotary sedimentation plate base, wherein slides to be loaded or loaded are adaptively received in the slide slots; A slide loading mechanism, which is located near the slide entrance of the rotary sedimentation tray and pushes the slide to be loaded into the rotary sedimentation tray from the slide entrance; A cleaning and sample-adding integrated device is provided beside the rotating sedimentation plate. The cleaning and sample-adding integrated device includes a cleaning liquid component and a sample-adding component. The cleaning liquid component and the sample-adding component respectively add liquid to two different sedimentation cups simultaneously with the intermittent rotation of the turntable body. A slide discharging mechanism is provided near a slide exit position of the rotary sedimentation plate, and moves the loaded slide out from the slide exit position; The temperature control assembly includes a temperature control element and a heating device connected to the temperature control element, the heating device is arranged at a position of the sedimentation plate seat corresponding to the sedimentation cup accommodating groove, and the temperature control element includes a resistance regulator; The sedimentation cup accommodating groove and the glass slide card slot are arranged at intervals. When the turntable body rotates intermittently, the glass slide inlet, the glass slide outlet and the glass slide card slot at the cleaning and sample addition inlet are connected to the space between the sedimentation cup accommodating groove.

2. The constant temperature fully automated cell preparation integrated device according to claim 1, characterized in that: The outer wall and bottom of the sedimentation plate seat are provided with a heat-insulating layer. The inner layer of the heat-insulating layer close to the turntable body is provided with heat-insulating cotton, while the outer layer away from the turntable body is provided with a metal cladding.

3. The constant temperature fully automated cell preparation integrated device according to claim 1, characterized in that: The temperature control component controls the temperature of the sedimentation cup in the sedimentation cup accommodating tank to be 20-60°C.

4. The constant temperature fully automated cell preparation integrated device according to claim 1, characterized in that: The slide adding mechanism includes a slide box with an opening and a pushing device. The pushing device pushes the slides stored in the slide box to the slide entrance position through the opening, and then the slides enter the slide card slot of the rotating sedimentation plate from the slide entrance.

5. The constant temperature fully automated cell preparation integrated device according to claim 1, characterized in that: The cleaning and sample loading integrated device further comprises a robotic arm, one end of which is connected to the Z-axis adjustment mechanism, and the other end of which is connected to the fluid of the cleaning liquid component and the sample loading component; The Z-direction adjustment mechanism reciprocates in the vertical direction to adjust the relative height of the robotic arm with respect to the turntable body.

6. The constant temperature fully automated cell preparation integrated device according to claim 5, characterized in that: The robotic arm has an arc-shaped portion.

7. The constant temperature fully automated cell preparation integrated device according to claim 1, characterized in that: The sheet discharging mechanism comprises a sheet discharging fork and a driving mechanism connected to the sheet discharging fork.

8. A method for using the constant temperature fully automated cell preparation integrated device according to any one of claims 1 to 7, characterized in that: The steps include: Step S1: The slide to be loaded enters the rotating sedimentation tray through the slide inlet and rotates intermittently along with the turntable body; Step S2: The cleaning liquid component and the sample loading component in the cleaning and loading integrated device add liquid to two different sedimentation cups respectively along with the intermittent rotation of the turntable body. At this time, the sample loading component adds sample to the glass slide to be loaded through the sedimentation cup, while the cleaning component cleans the sedimentation cup that has been loaded with sample, and the loaded glass slide is removed from the slide outlet.

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