A multi-environmental factor combined exposure device suitable for sea cucumbers

Abstract

The invention discloses a multi-environmental factor combined exposure device suitable for sea cucumbers, characterized in that: the device comprises a wave-making water tank, two ends of the wave-making water tank are respectively provided with a wave-making mechanism and a lifting drive mechanism, and a heater is also provided in the wave-making water tank, the lifting drive mechanism can drive a sampling tube with an electromagnetic valve to move longitudinally, the top end of the sampling tube is connected to the inlet end of an online water-oil concentration detector through a pipeline with a peristaltic pump, and the outlet end of the online water-oil concentration detector is connected in parallel with a plurality of water distribution tanks through a pipeline with an electromagnetic valve, the combined exposure device also comprises a water bath exposure box, a plurality of sea cucumber exposure boxes are arranged in the water bath exposure box, each water distribution tank is connected to a sea cucumber exposure box through a pipeline with an electromagnetic valve, a heater and a circulation pump are also provided in the water bath exposure box, and an automatic feeding mechanism is also provided above the water bath exposure box.

Description

Technical Field

[0001] The invention relates to the fields of sea cucumber farming and environmental impact assessment, in particular to a multi-environmental factor combined exposure device suitable for sea cucumbers. Background Art

[0002] In recent years, oil spills have occurred frequently in my country's coastal waters, causing a large amount of petroleum hydrocarbon compounds to enter the ocean waters, seriously threatening the health of the marine ecological environment and the survival of marine organisms in my country's coastal waters. At the same time, human society and industrial activities have caused a sharp increase in the emission of greenhouse gases such as CO2, causing global warming to gradually worsen, which in turn has led to an abnormal increase in offshore seawater temperature and a significant decrease in marine dissolved oxygen. Studies have shown that changes in temperature, light and seawater disturbance will affect the behavior of oil spills in seawater and changes in solubility components, and their own changes will also directly affect the physiological behavior of sea cucumbers. Sea cucumber farming areas are mainly distributed in the coastal areas of the Yellow Sea and Bohai Sea and are extremely susceptible to changes in environmental factors. Changes in the above-mentioned multiple environmental factors will have a huge impact on the sea cucumber farming industry, but at present, research on the impact mechanism of the combined stress caused by the changes in the above-mentioned multiple environmental factors on marine organisms is very rare, mainly due to the fact that there is currently no experimental device that can simulate the combined exposure of multiple environmental factors such as oil spill pollution, high temperature, dissolved oxygen changes, light and seawater disturbance.

[0003] Due to the special properties of petroleum hydrocarbons such as volatility, degradation and insolubility, the behavior and solubility components of marine oil spills in different spatial layers of seawater vary differently. How to effectively simulate the physiological effects of oil spills in different spatial layers of seawater on sea cucumbers under real conditions has become a major problem. Moreover, among the three main methods of water exposure, static water exposure does not replenish exposure fluid and cannot conduct long-term exposure experiments; the concentration of semi-static water exposure will fluctuate repeatedly within a certain period of time, affecting the experimental results; only flowing water exposure can ensure the accuracy of the experimental results; during the exposure experiment, the biological rhythm of sea cucumbers also needs to remain stable, and an exposure device that can meet the requirements of automatic feeding and timed illumination in the laboratory is urgently needed. Summary of the invention

[0004] The present invention aims to solve the above-mentioned deficiencies in the prior art and proposes an exposure device that can maintain the biological rhythm of sea cucumbers, use a flowing water exposure method, and simulate the effects of combined exposure of multiple environmental factors such as oil spill pollution, high temperature, dissolved oxygen changes and seawater disturbance on the growth of sea cucumbers at different seawater spatial levels.

[0005] The technical solution of the present invention is: a multi-environmental factor combined exposure device suitable for sea cucumbers, characterized in that: the device comprises a wave-making water tank 1, and the two ends of the wave-making water tank 1 are respectively provided with a wave-making mechanism and a lifting drive mechanism, and a heater 2 is also provided in the wave-making water tank 1, and the lifting drive mechanism can drive the sampling tube 4 with a solenoid valve 3 to move longitudinally, and the top of the sampling tube 4 is connected to the inlet end of the water-oil concentration online detector 6 through a pipeline with a peristaltic pump 5, and the outlet end of the water-oil concentration online detector 6 is connected to the inlet end of the water-oil concentration online detector 6 through a pipeline with a solenoid valve 3. In parallel with multiple water distribution tanks 7, the combined exposure device also includes a water bath exposure box 8, multiple sea cucumber exposure boxes 9 are arranged in the water bath exposure box 8, each water distribution tank 7 is connected to a sea cucumber exposure box 9 through a pipeline with a solenoid valve 3, and a heater 2 and a circulating pump 10 are also arranged in the water bath exposure box 8. An automatic feeding mechanism is also arranged above the water bath exposure box 8. The exposure device also includes a nitrogen cylinder 11 and an oxygen cylinder 12, and the nitrogen cylinder 11 and the oxygen cylinder 12 are respectively connected in parallel with all the water distribution tanks 7 through pipelines with gas flow control valves.

[0006] The wave-making mechanism includes a wave-making motor 13 fixedly supported on the wave-making water tank 1, and a cam 14 is provided at the output end of the wave-making motor 13. The cam 14 is hinged to one end of a wave-making connecting rod 15, and the other end of the wave-making connecting rod 15 is rotatably connected to a wave-making plate 16, and the wave-making plate 16 is slidably connected to a slide rail 17 provided on the wave-making water tank 1.

[0007] The lifting drive mechanism includes a lifting motor 18 fixedly supported on the wave-making water tank 1, and the output end of the lifting motor 18 is connected to a gear rotatably supported in a bracket 19 through a pulley transmission pair, and the gear is meshed with a rack 20 slidably connected in the bracket 19, and a support platform 21 is connected to the rack 20. The sampling tube 4 is fixedly supported on the support platform 21, and the bracket 19 is arranged in the wave-making water tank 1.

[0008] The automatic bait throwing mechanism includes a support frame 22, and the two ends of the support frame 22 are rotatably supported by an active roller 23 and a passive roller 24 respectively. The active roller 23 and the passive roller 24 jointly support a conveyor belt 25, and a bait throwing platform 26 is provided on the conveyor belt 25. An automatic bait throwing device 27 is provided on the bait throwing platform 26. A bait throwing motor 28 is also provided on the support frame 22, and the output end of the bait throwing motor 28 is connected to the active roller 23 through a pulley transmission pair. A lighting lamp with a timer is also provided at the bottom of the support frame 22.

[0009] The water distribution tank 7 is provided with a pH probe 29 and a dissolved oxygen probe 30 .

[0010] Compared with the prior art, the present invention has the following advantages:

[0011] This type of structural form of a multi-environmental factor combined exposure device suitable for sea cucumbers has a simple structure, ingenious design, and reasonable layout. It is designed with a special structure in view of the current status of traditional sea cucumber exposure research devices. It can maintain the biological rhythm of sea cucumbers, and using the exposure method of flowing water exposure, it can simulate multiple environmental factors such as oil spill pollution, high temperature, dissolved oxygen changes and seawater disturbances at different seawater spatial levels, so as to conduct scientific research on the impact of the above-mentioned multi-parameter combined exposure on the growth of sea cucumbers. The manufacturing process of this exposure device is simple and the manufacturing cost is low. Therefore, it can be said that it has many advantages and is particularly suitable for promotion and application in this field. Its market prospects are very broad. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic structural diagram of an embodiment of the present invention. DETAILED DESCRIPTION

[0013] The specific implementation of the present invention will be described below with reference to the accompanying drawings. Figure 1 As shown: A multi-environmental factor combined exposure device suitable for sea cucumbers, comprising a wave-making water tank 1, wherein both ends of the wave-making water tank 1 are respectively provided with a wave-making mechanism and a lifting drive mechanism, and a heater 2 is also provided in the wave-making water tank 1, wherein the lifting drive mechanism can drive a sampling tube 4 with an electromagnetic valve 3 to move longitudinally, wherein the top end of the sampling tube 4 is connected to the inlet end of an online water-oil concentration detector 6 through a pipeline with a peristaltic pump 5, and the outlet end of the online water-oil concentration detector 6 is connected in parallel with a plurality of water distribution tanks 7 through a pipeline with an electromagnetic valve 3, The combined exposure device also includes a water bath exposure box 8, in which a plurality of sea cucumber exposure boxes 9 are arranged, each water distribution box 7 is connected to a sea cucumber exposure box 9 through a pipeline with a solenoid valve 3, and a heater 2 and a circulation pump 10 are also arranged in the water bath exposure box 8, and an automatic feeding mechanism is also arranged above the water bath exposure box 8, and the exposure device also includes a nitrogen cylinder 11 and an oxygen cylinder 12, and the nitrogen cylinder 11 and the oxygen cylinder 12 are respectively connected in parallel with all the water distribution boxes 7 through pipelines with gas flow control valves.

[0014] The wave-making mechanism includes a wave-making motor 13 fixedly supported on the wave-making water tank 1, and a cam 14 is provided at the output end of the wave-making motor 13. The cam 14 is hinged to one end of a wave-making connecting rod 15, and the other end of the wave-making connecting rod 15 is rotatably connected to a wave-making plate 16, and the wave-making plate 16 is slidably connected to a slide rail 17 provided on the wave-making water tank 1.

[0015] The lifting drive mechanism includes a lifting motor 18 fixedly supported on the wave-making water tank 1, and the output end of the lifting motor 18 is connected to a gear rotatably supported in a bracket 19 through a pulley transmission pair, and the gear is meshed with a rack 20 slidably connected in the bracket 19, and a support platform 21 is connected to the rack 20. The sampling tube 4 is fixedly supported on the support platform 21, and the bracket 19 is arranged in the wave-making water tank 1.

[0016] The automatic bait throwing mechanism includes a support frame 22, and the two ends of the support frame 22 are rotatably supported by an active roller 23 and a passive roller 24 respectively. The active roller 23 and the passive roller 24 jointly support a conveyor belt 25, and a bait throwing platform 26 is provided on the conveyor belt 25. An automatic bait throwing device 27 is provided on the bait throwing platform 26. A bait throwing motor 28 is also provided on the support frame 22, and the output end of the bait throwing motor 28 is connected to the active roller 23 through a pulley transmission pair. A lighting lamp with a timer is also provided at the bottom of the support frame 22.

[0017] The water distribution tank 7 is provided with a pH probe 29 and a dissolved oxygen probe 30 .

[0018] The working process of the multi-environmental factor combined exposure device suitable for sea cucumbers in the embodiment of the present invention is as follows: first, seawater and exposure oil products are added to the wave-making water tank 1, and then a command is issued through the control system of the device to control the wave-making mechanism to work, so as to create waves of a certain frequency in the wave-making water tank 1. At the same time, the heater 2 will also heat the water in the wave-making water tank 1. During this process, the sampling tube 4 of the lifting drive mechanism moves to make it hover at different water depths, and the peristaltic pump 5 is used to provide power to input the collected water samples into the water-oil concentration online detector 6, so as to detect the oil concentration in the water at different depths. After the detection, the solenoid valves 3 on different water distribution tanks 7 are controlled to input the water samples at different depths into different water distribution tanks 7. According to the data detected by the pH probe 29 and the dissolved oxygen probe 30 in each water distribution tank 7, the aeration rate of nitrogen and oxygen in each water distribution tank 7 is adjusted (by adjusting the gas flow control valves on the nitrogen cylinder 11 and the oxygen cylinder 12).

[0019] After the aeration work is completed, the solenoid valve 3 connected to the pipeline between the water distribution tank 7 and the sea cucumber exposure box 9 is opened, and the water sample treated with aeration is input into the sea cucumber exposure box 9. The test sea cucumbers are cultured in each sea cucumber exposure box 9. By observing the specific breeding conditions of the test sea cucumbers in different water sample environments, the oil concentration in the polluted seawater of different water depths can be judged; during the breeding process, the heater 2 located in the water bath exposure box 8 is turned on, and the circulating pump 10 is started at the same time, so that the temperature of the water body in the water bath exposure box 8 can be quickly and evenly adjusted, thereby achieving the purpose of indirectly adjusting the water temperature in all sea cucumber exposure boxes 9;

[0020] During the process of cultivating sea cucumbers, the automatic feeding mechanism located above the multiple sea cucumber exposure boxes 9 works to feed the sea cucumbers cultivated in each sea cucumber exposure box 9;

[0021] The working process of the wave-making mechanism is as follows: the wave-making motor 13 is controlled by the control system to work, the wave-making motor 13 drives the cam 14 to rotate, and then drives the wave-making connecting rod 15 to swing, and the wave-making connecting rod 15 will eventually drive the wave-making plate 16 to reciprocate along the slide rail 17, and finally simulate waves of a certain frequency in the wave-making water tank 1;

[0022] The working process of the lifting drive mechanism is as follows: the lifting motor 18 is controlled by the control system to work, and the lifting motor 18 drives the gear to rotate through the pulley transmission pair, thereby driving the rack 20 meshing with the gear to make longitudinal linear motion in the bracket 19, and finally changing the horizontal height of the sampling tube 4 fixedly supported on the supporting platform 21, so as to achieve the purpose of sampling the sampling tube 4 at different water depths;

[0023] The working process of the automatic feeding mechanism is as follows: the control system controls the feeding motor 28 to work, and the feeding motor 28 drives the active roller 23 to rotate through the pulley transmission pair, thereby driving the conveyor belt 25 to move. The feeding platform 26 can be controlled to stop above a certain sea cucumber exposure box 9 by controlling the number of revolutions of the feeding motor 28. When the feeding motor 28 stops, the control system receives a signal and controls the automatic feeding device 27 to work, and the bait pre-placed in the automatic feeding device 27 is placed in the corresponding sea cucumber exposure box 9, and this is repeated until all the automatic feeding devices 27 complete the feeding operation in all the sea cucumber exposure boxes 9 in turn.

Claims

1. A multi-environmental factor combined exposure device suitable for sea cucumbers, characterized in that: The device comprises a wave-making water tank (1), wherein both ends of the wave-making water tank (1) are respectively provided with a wave-making mechanism and a lifting drive mechanism, and a heater (2) is also provided inside the wave-making water tank (1), wherein the lifting drive mechanism can drive a sampling tube (4) with an electromagnetic valve (3) to move longitudinally, wherein the top end of the sampling tube (4) is connected to the inlet end of an online water-oil concentration detector (6) via a pipeline with a peristaltic pump (5), and the outlet end of the online water-oil concentration detector (6) is connected in parallel with a plurality of water distribution tanks (7) via a pipeline with an electromagnetic valve (3). The combined exposure device further comprises A water bath exposure box (8), wherein a plurality of sea cucumber exposure boxes (9) are arranged in the water bath exposure box (8), each water distribution box (7) is connected to a sea cucumber exposure box (9) via a pipeline with a solenoid valve (3), and a heater (2) and a circulation pump (10) are also arranged in the water bath exposure box (8), and an automatic feeding mechanism is also arranged above the water bath exposure box (8), and the exposure device also includes a nitrogen cylinder (11) and an oxygen cylinder (12), and the nitrogen cylinder (11) and the oxygen cylinder (12) are respectively connected in parallel with all the water distribution boxes (7) via pipelines with gas flow control valves, The automatic bait throwing mechanism comprises a support frame (22), wherein two ends of the support frame (22) respectively support an active roller (23) and a passive roller (24) for rotation, wherein the active roller (23) and the passive roller (24) jointly support a conveyor belt (25), wherein a bait throwing platform (26) is arranged on the conveyor belt (25), wherein an automatic bait throwing device (27) is arranged on the bait throwing platform (26), wherein a bait throwing motor (28) is also arranged on the support frame (22), wherein an output end of the bait throwing motor (28) is connected to the active roller (23) via a belt pulley transmission pair, and an illumination lamp with a timer is also arranged at the bottom of the support frame (22).

2. The multi-environmental factor combined exposure device suitable for sea cucumbers according to claim 1, characterized in that: The wave-making mechanism comprises a wave-making motor (13) fixedly supported on a wave-making water tank (1), a cam (14) being provided at the output end of the wave-making motor (13), the cam (14) being hinged to one end of a wave-making connecting rod (15), the other end of the wave-making connecting rod (15) being rotatably connected to a wave-making plate (16), and the wave-making plate (16) being slidably connected to a slide rail (17) provided on the wave-making water tank (1).

3. The multi-environmental factor combined exposure device suitable for sea cucumbers according to claim 1, characterized in that: The lifting drive mechanism comprises a lifting motor (18) fixedly supported on the wave-generating water tank (1); the output end of the lifting motor (18) is connected to a gear rotatably supported in a bracket (19) via a pulley transmission pair; the gear is meshed with a rack (20) slidably connected in the bracket (19); a support platform (21) is connected to the rack (20); the sampling tube (4) is fixedly supported on the support platform (21), and the bracket (19) is arranged in the wave-generating water tank (1).

4. The multi-environmental factor combined exposure device suitable for sea cucumbers according to claim 1, characterized in that: A pH probe (29) and a dissolved oxygen probe (30) are provided in the water distribution tank (7).

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