A slide pretreatment system

Abstract

The present invention discloses a slide pretreatment system, including a system body, the system body including a bottom plate, a shell component covered on the top of the bottom plate, a power supply component arranged between the shell component and the bottom plate, a main control drive component, a solenoid valve drive component and a constant temperature bath component, the main control drive component including a liquid addition peristaltic pump and a liquid discharge peristaltic pump fixed on the bottom plate, the solenoid valve drive component including a reagent input channel switching solenoid valve, a waste liquid emptying channel switching solenoid valve and a reagent distribution channel switching solenoid valve, the constant temperature bath component including a fixed frame fixed to the top of the bottom plate at opposite ends, a sample table connected between the two fixed frames by a rotating shaft, and a plurality of groups of sample constant temperature parts fixed on the sample table, and a rocker mechanism for driving the sample table to rotate is provided between the bottom end of the sample table and the bottom plate. The present invention has a reasonable structural design, is convenient and quick to use, can effectively reduce the error of the test result, and is conducive to market promotion and application.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a slide pretreatment system. Background Art

[0002] In situ hybridization refers to the process of using a specifically labeled known sequence nucleic acid as a probe to hybridize with nucleic acids in cells or tissue sections, thereby accurately and quantitatively locating a specific nucleic acid sequence. The specific principle is: based on the principle of complementary pairing of nucleic acid bases, a hapten-labeled DNA or RNA probe is complementary paired with a denatured single-stranded nucleic acid sequence, and the hapten is detected by an antibody with a fluorescent group, or the probe is directly labeled with a fluorescent group and combined with the target sequence, and finally a fluorescent microscope is used to directly observe the distribution of the target sequence in the cell nucleus, chromosome or section tissue. At present, this technology has been widely used in many fields such as animal and plant genome structure research, chromosome fine structure variation analysis, virus infection analysis, human prenatal diagnosis, tumor genetics and genome evolution research.

[0003] However, before the in situ hybridization instruments currently used in the market are used, technicians need to complete a large number of preliminary processing steps, which not only wastes a lot of time and increases the workload of operators, but also causes large errors in the final test results due to the addition of a large number of human factors, thus failing to meet people's requirements. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a slide pretreatment system which has a reasonable structural design, is convenient and quick to use, and can effectively reduce the error of test results.

[0005] The present invention adopts the following technical solution:

[0006] A slide pretreatment system includes a system body, the system body includes a bottom plate, a shell component covered on the top of the bottom plate, a power supply component arranged between the shell component and the bottom plate, a main control drive component, an electromagnetic valve drive component and a constant temperature bath component, the power supply component is fixed on one side of the top of the bottom plate, the main control drive component includes a liquid addition peristaltic pump and a liquid discharge peristaltic pump fixed on the side of the bottom plate opposite to the power supply component, the electromagnetic valve drive component includes a reagent input channel switching electromagnetic valve, a waste liquid emptying channel switching electromagnetic valve and a thermostatic bath component fixed on the power supply component The reagent distribution channel switching solenoid valve is connected, the output end of the reagent input channel switching solenoid valve is connected to the input end of the liquid adding peristaltic pump, the output end of the liquid adding peristaltic pump is connected to the input end of the reagent distribution channel switching solenoid valve, the input end of the waste liquid emptying channel switching solenoid valve is connected to the output end of the liquid discharge peristaltic pump, the constant temperature bath assembly includes fixed frames fixed at opposite ends of the top of the bottom plate, a sample table rotatably connected between the two fixed frames by a rotating shaft, and several groups of sample constant temperature parts fixed on the sample table, and a rocker mechanism for driving the sample table to rotate is provided between the bottom end of the sample table and the bottom plate.

[0007] As a preferred technical solution of the present invention, the top end of the shell component is provided with an opening, the opening is covered with a glass cover, one side of the glass cover is hinged to the corresponding position of the shell component, and the other end of the glass cover is a movable end.

[0008] As a preferred technical solution of the present invention, a fixing plate is provided at one end of the top of the bottom plate, and a USB interface and a VGR video interface are fixedly provided on the fixing plate.

[0009] As a preferred technical solution of the present invention, the main control drive component also includes a main control drive circuit board and a power socket fixed to one side of the power supply component and electrically connected to the power supply component respectively, and the liquid adding peristaltic pump and the liquid discharging peristaltic pump are respectively connected to the main control drive circuit board through wires.

[0010] As a preferred technical solution of the present invention, the solenoid valve drive assembly also includes a solenoid valve drive circuit board, the top of the base plate is provided with an outer frame covering the outside of the liquid adding peristaltic pump and the liquid discharging peristaltic pump, the solenoid valve drive circuit board is fixed on the outer frame, the reagent input channel switching solenoid valve, the waste liquid emptying channel switching solenoid valve and the reagent distribution channel switching solenoid valve are respectively connected to the solenoid valve drive circuit board signal, and the solenoid valve drive circuit board is also connected to the main control drive circuit board signal.

[0011] As a preferred technical solution of the present invention, the thermostatic bath assembly also includes a plurality of thermostatic bath circuit control boards fixed to the bottom of the sample stage and arranged one-to-one with the sample thermostatic components, and the plurality of thermostatic bath circuit control boards are respectively connected to the main control drive circuit board signal.

[0012] As a preferred technical solution of the present invention, a plurality of thermostatic baths are provided at the top of the sample thermostatic element, and a sealing cover for covering the thermostatic baths is also provided at the top of the sample thermostatic element. A reagent channel assembly corresponding to the thermostatic baths and communicating with the interior of the thermostatic baths is provided on the side wall of the sample thermostatic element, and the reagent channel assembly includes a liquid inlet joint and a liquid outlet joint, the liquid inlet joint is connected to the output end of the reagent distribution channel switching solenoid valve, and the liquid outlet joint is connected to the input end of the liquid discharge peristaltic pump. A temperature sensor for detecting the internal temperature of the thermostatic bath is also provided on the side wall of the sample thermostatic element, a heating film and a heating film PCB board are fixedly provided on the outer side of the bottom wall of the sample thermostatic element, and a pad, a refrigeration fin, a heat sink mounting plate and a heat sink are fixedly provided on the bottom end of the heating film in sequence from top to bottom, and the heating film PCB board and the temperature sensor are respectively connected to the thermostatic bath circuit control board signal.

[0013] As a preferred technical solution of the present invention, the rocker mechanism includes a screw stepper motor, a connecting rod and a "T"-shaped connecting frame, a lower bearing is fixedly provided on the output end of the screw stepper motor, the bottom end of the connecting rod is hinged on the lower bearing, the top end of the connecting frame is fixed to the bottom end of the sample table, an upper bearing is fixedly provided on the bottom end of the connecting frame, and the top end of the connecting rod is hinged on the upper bearing.

[0014] The beneficial effects of the present invention are:

[0015] The invention has reasonable structural design, simple and convenient operation, high degree of automation, and can effectively avoid accidental errors in test results caused by excessive manual operation, thereby improving test accuracy; at the same time, the rocker mechanism can greatly improve the hybridization reaction effect and improve the use effect of the system body. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the internal three-dimensional structure of the system body of the present invention;

[0017] Figure 2 for Figure 1 The main structure diagram of

[0018] Figure 3 for Figure 1 The rear view structure diagram;

[0019] Figure 4 for Figure 1 The side view of the structure;

[0020] Figure 5 for Figure 2 Schematic diagram of the cross section at DD;

[0021] Figure 6 It is a schematic diagram of the structure of the housing assembly of the present invention;

[0022] Figure 7 It is a schematic diagram of the structure between the base plate and the main control drive assembly of the present invention;

[0023] Figure 8 It is a schematic diagram of the structure between the base plate and the solenoid valve drive assembly of the present invention;

[0024] Fig. 9 It is a schematic diagram of the three-dimensional structure of the sample thermostat of the present invention;

[0025] Fig.10 for Fig. 9 The main structure diagram of

[0026] Fig.11 for Fig. 9 A side view structural schematic diagram of;

[0027] Explanation of symbols in the figure:

[0028] Base plate 1, shell assembly 2, power supply assembly 3, liquid addition peristaltic pump 4, liquid discharge peristaltic pump 5, reagent input channel switching solenoid valve 6, waste liquid emptying channel switching solenoid valve 7, reagent distribution channel switching solenoid valve 8, fixing frame 9, sample stage 10, sample constant temperature component 11, glass cover 12, fixing plate 13, USB interface 14, VGR video interface 15, main control drive circuit board 16, power socket 17, solenoid valve drive circuit board 18, outer frame 19, constant temperature bath circuit control board 20, constant temperature bath 21, sealing cover 22, reagent channel assembly 23, temperature sensor 24, heating film 25, heating film PCB board 26, refrigeration plate 27, heat sink mounting plate 28, heat sink 29, screw stepper motor 30, connecting rod 31, connecting frame 32, lower bearing 33, upper bearing 34. DETAILED DESCRIPTION

[0029] The present invention will now be described in further detail with reference to the accompanying drawings.

[0030] like Figures 1 to 11 As shown, a slide pretreatment system includes a system body, the system body includes a base plate 1, a shell component 2 covered on the top of the base plate 1, a power supply component 3 arranged between the shell component 2 and the base plate 1, a main control drive component, a solenoid valve drive component and a constant temperature bath component, the power supply component 3 is fixed on one side of the top of the base plate 1, the main control drive component includes a liquid adding peristaltic pump 4 and a liquid discharging peristaltic pump 5 fixed on the base plate 1 on the side opposite to the power supply component 3, specifically, during preparation, the shell component 2 is provided with reserved openings adapted to the liquid adding peristaltic pump 4 and the liquid discharging peristaltic pump 5 at positions corresponding to the liquid adding peristaltic pump 4 and the liquid discharging peristaltic pump 5, so that the liquid adding peristaltic pump 4 and the liquid discharging peristaltic pump 5 can be mutually clamped with the shell component 2 during assembly;

[0031] The solenoid valve driving assembly includes a reagent input channel switching solenoid valve 6, a waste liquid emptying channel switching solenoid valve 7, and a reagent distribution channel switching solenoid valve 8 fixed to the top of the power supply assembly 3 through a right-angle fixing plate, the output end of the reagent input channel switching solenoid valve 6 is connected to the input end of the liquid adding peristaltic pump 4, the output end of the liquid adding peristaltic pump 4 is connected to the input end of the reagent distribution channel switching solenoid valve 8, and the input end of the waste liquid emptying channel switching solenoid valve 7 is connected to the output end of the liquid discharge peristaltic pump 5. When in use, the external reagent is connected to the input end of the reagent input channel switching solenoid valve 6 through a pipeline, and the output end of the waste liquid emptying channel switching solenoid valve 7 is connected to an external waste liquid collector through a pipeline; more specifically, in this embodiment, during preparation, the housing assembly 2 is provided with a reserved opening for easy connection with external components at the input end position of the reagent input channel switching solenoid valve 6 and the output end position of the waste liquid emptying channel switching solenoid valve 7;

[0032] The thermostatic bath assembly includes a fixing frame 9 fixed to opposite ends of the top of the base plate 1, a sample table 10 rotatably connected between the two fixing frames 9 by a rotating shaft, and a plurality of sample thermostatic components 11 fixed on the sample table 10. Specifically, in the present embodiment, the number of the sample thermostatic components is 3. In a specific application process, technicians in this field can determine the specific number of the sample thermostatic components according to actual needs; for example, two, four, five, etc.; further, a rocker mechanism is provided between the bottom end of the sample table 10 and the base plate 1 for driving the sample table 10 to rotate relative to the fixing frame 9.

[0033] More specifically, the top end of the housing component 2 is provided with an opening (not shown in the figure), and the opening is covered with a glass cover 12, one side of the glass cover 12 is hinged to the corresponding position of the housing component 2, and the other end of the glass cover 12 is a movable end;

[0034] Specifically, a fixing plate 13 is provided at one end of the top of the bottom plate 1, and two USB interfaces 14 and a VGR video interface 15 are fixedly provided on the fixing plate 13; the VGR video interface 15 is used to connect to an external PC terminal;

[0035] More specifically, the main control drive assembly also includes a main control drive circuit board 16 and a power socket 17 fixed to one side of the power supply assembly 3 and electrically connected to the power supply assembly 3 respectively, the liquid addition peristaltic pump 4 and the liquid discharge peristaltic pump 5 are respectively connected to the main control drive circuit board 16 through wires, and the USB interface 14 and the VGR video interface 15 are also respectively connected to the main control drive circuit board 16 through wires;

[0036] Further specifically, the solenoid valve drive assembly also includes a solenoid valve drive circuit board 18, the top of the bottom plate 1 is provided with an outer frame 19 that covers the outside of the liquid addition peristaltic pump 4 and the liquid discharge peristaltic pump 5, the solenoid valve drive circuit board 18 is fixed on the outer frame 19, the input channel switching solenoid valve 6, the waste liquid emptying channel switching solenoid valve 7 and the reagent distribution channel switching solenoid valve 8 are respectively connected to the solenoid valve drive circuit board 18 by signal, and the solenoid valve drive circuit board 18 is also connected to the main control drive circuit board 16 by signal;

[0037] Further specifically, the thermostatic bath assembly further includes a plurality of thermostatic bath circuit control boards 20 fixed to the bottom end of the sample stage 10 and arranged one-to-one with the sample thermostatic elements 11. Specifically, in this embodiment, the number of the thermostatic bath circuit control boards 20 is also three; the three thermostatic bath circuit control boards 20 are respectively connected to the main control drive circuit board 16 by signal;

[0038] Further specifically, the top of the sample thermostat 11 is provided with a plurality of thermostats 21. Specifically, in the present embodiment, the number of thermostats 21 in each sample thermostat 11 is two, and the top of the sample thermostat 11 is also provided with a sealing cover 22 for covering the thermostat 21. The side wall of the sample thermostat 11 is provided with a reagent channel assembly 23 which is arranged one-to-one with the thermostat 21 and communicates with the interior of the thermostat 21. The reagent channel assembly 23 includes a liquid inlet joint and a liquid outlet joint. The liquid inlet joint is connected to the reagent distribution channel switching solenoid valve 8. The output end is connected, the liquid outlet joint is connected with the input end of the drainage peristaltic pump 5, and a temperature sensor 24 for detecting the internal temperature of the thermostatic bath 21 is also provided on the side wall of the sample thermostatic element 11. A heating film 25 and a heating film PCB board 26 are fixedly provided on the outer side of the bottom wall of the sample thermostatic element 11. A pad (not shown in the figure), a cooling fin 27, a heat sink mounting plate 28 and a heat sink 29 are fixedly provided at the bottom end of the heating film 25 in sequence from top to bottom. The heating film PCB board 26 and the temperature sensor 24 are respectively connected to the thermostatic bath circuit control board 20 for signal connection;

[0039] More specifically, the rocker mechanism includes a screw stepper motor 30, a connecting rod 31 and a "T"-shaped connecting frame 32, the output end of the screw stepper motor 30 is fixedly provided with a lower bearing 33, the bottom end of the connecting rod 31 is hinged to the lower bearing 33, the top end of the connecting frame 32 is fixed to the bottom end of the sample table 10, the bottom end of the connecting frame 32 is fixedly provided with an upper bearing 34, and the top end of the connecting rod 31 is hinged to the upper bearing 34.

[0040] The use process of the present invention is briefly described as follows:

[0041] When in use, first place the glass slide to be tested on the slide rack, then place the entire slide rack in the constant temperature bath 21 and cover it with the sealing cover 22, control the reagent input channel switching solenoid valve 6 and the reagent distribution channel switching solenoid valve 8 through the solenoid valve driving circuit board 18 to open the corresponding channels, and then start the liquid adding peristaltic pump 4 through the main control driving circuit board 16 to transport the corresponding reagent to the corresponding constant temperature bath 21 with the slide rack; then start the heating film 25 through the constant temperature bath circuit control board 20 to heat the corresponding constant temperature bath 21; finally, start the screw stepper motor 30 as needed to drive the connecting rod 31 to reciprocate up and down, thereby driving the sample stage 10 and the sample constant temperature component on the sample stage 10 to swing back and forth, thereby improving the reaction effect.

[0042] After the reaction is completed, the waste liquid in the corresponding thermostatic bath 21 can be discharged through the waste liquid discharge channel switching solenoid valve 7 and the liquid discharge peristaltic pump 5 .

[0043] Finally, it should be noted that these embodiments are only used to illustrate the present invention and do not limit the scope of the present invention. In addition, for those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. The obvious changes or modifications derived from them are still within the scope of protection of the present invention.

Claims

1. A slide pretreatment system, comprising a system body, characterized in that: The system body comprises a base plate (1), a housing component (2) arranged on the top of the base plate (1), a power supply component (3) arranged between the housing component (2) and the base plate (1), a main control drive component, a solenoid valve drive component and a constant temperature bath component, wherein the power supply component (3) is fixed on one side of the top of the base plate (1), the main control drive component comprises a liquid addition peristaltic pump (4) and a liquid discharge peristaltic pump (5) fixed on the side of the base plate (1) opposite to the power supply component (3), and the solenoid valve drive component comprises a reagent input channel switching solenoid valve (6) and a waste liquid discharge channel switching solenoid valve (7) respectively fixed on opposite ends of the top of the base plate (1). 7) and a reagent distribution channel switching electromagnetic valve (8) fixed above the power supply assembly (3), the output end of the reagent input channel switching electromagnetic valve (6) is connected to the input end of the liquid adding peristaltic pump (4), the output end of the liquid adding peristaltic pump (4) is connected to the input end of the reagent distribution channel switching electromagnetic valve (8), the input end of the waste liquid emptying channel switching electromagnetic valve (7) is connected to the output end of the liquid discharge peristaltic pump (5), the constant temperature bath assembly includes a fixing frame (9) fixed to the top opposite ends of the bottom plate (1), a sample table (10) rotatably connected between the two fixing frames (9) by a rotating shaft, and a plurality of groups of fixing frames (10) fixed on the sample table (10) The sample thermostat (11) is provided between the bottom end of the sample stage (10) and the bottom plate (1), and a rocker mechanism for driving the sample stage (10) to rotate is provided. The top end of the housing component (2) is provided with an opening, and the opening is covered with a glass cover (12). One side of the glass cover (12) is hinged to the corresponding position of the housing component (2), and the other end of the glass cover (12) is a movable end. The main control drive component also includes a main control drive circuit board (16) and a power socket (17) fixed to one side of the power supply component (3) and electrically connected to the power supply component (3) respectively. The liquid addition peristaltic pump (4) and the liquid discharge peristaltic pump (5) are respectively connected to the main control driving circuit board (16) through wires, the solenoid valve driving component also includes a solenoid valve driving circuit board (18), the top of the bottom plate (1) is provided with an outer frame (19) which is arranged outside the liquid adding peristaltic pump (4) and the liquid discharging peristaltic pump (5), the solenoid valve driving circuit board (18) is fixed on the outer frame (19), the reagent input channel switching solenoid valve (6), the waste liquid emptying channel switching solenoid valve (7) and the reagent distribution channel switching solenoid valve (8) are respectively connected to the solenoid valve driving circuit board (18) by signal, and the solenoid valve driving circuit board (18) is also connected to the main control driving circuit board (16) by signal.

2. A slide pretreatment system according to claim 1, characterized in that: A fixing plate (13) is provided at one end of the top of the bottom plate (1), and a USB interface (14) and a VGR video interface (15) are fixedly provided on the fixing plate.

3. A slide pretreatment system according to claim 1, characterized in that: The thermostatic bath assembly further comprises a plurality of thermostatic bath circuit control boards (20) fixed to the bottom end of the sample platform (10) and arranged one-to-one corresponding to the sample thermostatic components (11); the plurality of thermostatic bath circuit control boards (20) are respectively connected to the main control drive circuit board (16) by signal.

4. A slide pretreatment system according to claim 3, characterized in that: The top of the sample thermostatic element (11) is provided with a plurality of thermostatic baths (21), and the top of the sample thermostatic element (11) is also provided with a sealing cover (22) for covering the thermostatic baths (21). The side wall of the sample thermostatic element (11) is provided with a reagent channel assembly (23) which is arranged one-to-one with the thermostatic baths (21) and communicates with the interior of the thermostatic baths (21). The reagent channel assembly (23) includes a liquid inlet joint and a liquid outlet joint, wherein the liquid inlet joint is communicated with the output end of the reagent distribution channel switching solenoid valve (8), and the liquid outlet joint is communicated with the liquid discharge peristaltic pump (5). The sample thermostat (11) is connected to an input end thereof, a temperature sensor (24) for detecting the internal temperature of the thermostat (21) is also provided on the side wall of the sample thermostat (11), a heating film (25) and a heating film PCB board (26) are fixedly provided on the outer side of the bottom wall of the sample thermostat (11), a pad, a cooling fin (27), a heat sink mounting board (28) and a heat sink (29) are fixedly provided on the bottom end of the heating film (25) in sequence from top to bottom, and the heating film PCB board (26) and the temperature sensor (24) are respectively connected to the thermostat circuit control board (20) for signal.

5. A slide pretreatment system according to claim 1, characterized in that: The rocker mechanism comprises a screw stepper motor (30), a connecting rod (31) and a T-shaped connecting frame (32); a lower bearing (33) is fixedly provided on the output end of the screw stepper motor (30); the bottom end of the connecting rod (31) is hinged on the lower bearing (33); the top end of the connecting frame (32) is fixed on the bottom end of the sample platform (10); an upper bearing (34) is fixedly provided on the bottom end of the connecting frame (32); and the top end of the connecting rod (31) is hinged on the upper bearing (34).

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