Integrated multi-stage sampling, preservation, and mixing device

Abstract

This invention provides an integrated multi-stage sample loading, preservation, and mixing device, comprising: a sampling box cover component (1), a sampling box connector (2), a sampling unit (4), and a sample mixing unit; the sampling unit (4) includes: sampling components (401); the number of sampling components (401) is multiple; the sampling components (401) are evenly arranged at equal intervals in the sampling unit (4); the sample mixing unit is connected to the sampling unit (4); the sampling box cover component (1) is connected to the sampling unit (4) through the sampling box connector (2); the sampling box cover component (1) and the sampling box connector (2) are detachably connected; the sampling unit (4) and the sampling box connector (2) are detachably connected. This invention solves the problem that different serum samples from ELISA plates need to be aspirated one by one, and this invention can greatly reduce the time for sample loading and mixing.

Description

Technical Field

[0001] This invention relates to the field of sample loading technology, specifically to an integrated multi-stage pipette loading, preservation, and mixing device, and more particularly to an integrated multi-stage pipette loading, preservation, and mixing device for molecular biology. Background Technology

[0002] Existing technologies often encounter the problem that different serum samples need to be aspirated one by one for each ELISA plate, which greatly increases the sample addition time and is not conducive to the smooth progress of sample addition. In addition, after serum or reagents are added, the samples often need to be manually aspirated into the reaction container, which can cause secondary contamination and is also inefficient.

[0003] When conducting experiments on samples, it is often necessary to control the amount of each sample added each time. Furthermore, it is often necessary to adjust environmental variables (such as temperature and air pressure). Existing technologies cannot adequately meet these requirements.

[0004] Patent document CN211697839U discloses a porous pneumatic precision sample dispensing box. Its key technical features include: a box body that can be fitted into a matching sample stage; a microfluidic channel inside the box body; a sample dispensing groove, a waste liquid groove, and a sample collection groove resonating with the microfluidic channel on the upper surface of the box body; each waste liquid groove and sample collection groove being connected to an air pump; and multiple sets of sample dispensing grooves. The lower surface of the box body has outlet holes and extraction holes resonating with the microfluidic channel. Each set of outlet holes and extraction holes is connected to the respective sets of sample dispensing grooves, waste liquid grooves, and sample collection grooves on the upper surface of the box body via the microfluidic channel. An outlet groove extending from the lower surface of the box body surrounds each set of outlet holes. An extraction groove extending from the lower surface of the box body, located outside the extraction holes, is provided between the extraction groove and the outlet groove for connection. This box features automatic precision reagent dispensing, waste liquid collection, and sample preparation functions. Through a disposable connection method, it eliminates the advantages of cross-contamination and secondary contamination. There is still room for improvement in the structure and performance of this patent. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the purpose of this invention is to provide an integrated multi-stage sampling, preservation, and mixing device.

[0006] According to the present invention, an integrated sampling, preservation and mixing device for adding samples by a multi-stage gun includes: a sampling box cover component 1, a sampling box connector 2, a sampling unit 4, and a sample mixing unit;

[0007] The sampling unit 4 includes: a sampling component 401;

[0008] The number of sampling components 401 is multiple;

[0009] The sampling components 401 are evenly and equidistantly arranged in the sampling unit 4;

[0010] The sample mixing unit is connected to the sampling unit 4;

[0011] The sampling box cover component 1 is connected to the sampling unit 4 via the sampling box connector 2;

[0012] The sampling box cover component 1 and the sampling box connector 2 are connected in a detachable manner;

[0013] The sampling unit 4 and the sampling box connector 2 are connected in a detachable manner.

[0014] Preferably, the number of sampling components 401 is 96;

[0015] The sampling components 401 are arranged in 8 rows at equal intervals in the sampling unit 4.

[0016] Preferably, the sample mixing unit includes: a first sample mixing unit 5 and a second sample mixing unit 7;

[0017] The first sample mixing unit 5 and the second sample mixing unit 7 are respectively connected to the sampling unit 4.

[0018] Preferably, it further includes: a hybrid unit display component;

[0019] The mixing unit display component is connected to the sample mixing unit;

[0020] The mixing unit display component includes: a first sample mixing unit display component 6 and a second sample mixing unit display component 8;

[0021] The first sample mixing unit display component 6 is connected to the first sample mixing unit 5;

[0022] The second sample mixing unit display component 8 is connected to the second sample mixing unit 7.

[0023] Preferably, the sampling unit 4 further includes: a regulating valve 402 and a container connecting pipeline;

[0024] The sampling component 401 is a sampling container;

[0025] The container connecting pipes are connected to the sampling container and the sample mixing unit, respectively.

[0026] Preferably, the regulating valve 402 includes: a main regulating valve 411 and a branch regulating valve 412;

[0027] The container connection pipeline includes: a container connection branch pipeline 403 and a container connection main pipeline 404;

[0028] The main regulating valve is located on the main pipeline connecting the container;

[0029] The branch regulating valve is installed on the branch pipe connecting the container.

[0030] Preferably, it further includes: a liquid pump component 11;

[0031] The liquid pump component is connected to the connection point between the container connection main pipeline and the sample mixing unit.

[0032] Preferably, it further includes: a pump and valve integrated control unit 17, a strategy storage and calculation unit 16, and a user input unit 15;

[0033] The pump and valve integrated control component 17 is connected to the liquid pump component 11, the main regulating valve 411, and the branch regulating valve 412 respectively.

[0034] The pump and valve integrated control component 17 is connected to the strategy storage and calculation component 16;

[0035] The strategy storage and calculation unit 16 is connected to the user input unit 15.

[0036] Preferably, it also includes: a sampling box storage cabinet 14;

[0037] The sampling unit 4 also includes: a slide rail 3;

[0038] The slide rail 3 is located at the bottom of the sampling unit 4;

[0039] The sampling box storage cabinet 14 includes: a sampling box storage cabinet compartment, a sampling box storage cabinet control unit 142, and a compressor outdoor unit 143;

[0040] The inner wall of the sampling box storage cabinet is provided with a sliding groove;

[0041] The slide groove cooperates with the slide rail 3;

[0042] The sampling box storage cabinet control unit 142 can control any environmental variable inside the sampling box storage cabinet:

[0043] -Temperature variable;

[0044] -Gas pressure variables;

[0045] The sampling box storage cabinet control unit 142 is connected to the compressor outdoor unit 143.

[0046] Preferably, the sampling box storage cabinet 14 further includes: a sampling box storage cabinet display screen 144 and a sampling box storage cabinet recycling component 141;

[0047] The sampling box storage cabinet recycling component 141 is located at the lower part of the sampling box storage cabinet 14;

[0048] The display screen 144 of the sampling box storage cabinet is located on the outer wall of the sampling box storage cabinet 14.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] 1. The present invention adopts a technical solution of equidistant arrangement of sampling components, which solves the problem that different serum samples need to be aspirated one by one in the ELISA plate, and greatly reduces the sample addition time.

[0051] 2. The present invention has a reasonable structural design and is easy to use, and can overcome the defects of the prior art;

[0052] 3. This invention allows for a very simple and convenient sample aspiration into the reaction vessel, without causing secondary contamination, thus improving experimental efficiency;

[0053] 4. This invention enables precise control over the amount of each sample added each time. It also allows for adjustment of environmental variables (such as temperature and air pressure) in the sampling box storage environment. Attached Figure Description

[0054] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0055] Figure 1 This is a schematic diagram of the integrated multi-gun sample preparation and mixing device of the present invention.

[0056] Figure 2 This is a schematic diagram illustrating the connection principle between the sampling container and the sample mixing unit in an embodiment of the present invention.

[0057] Figure 3 This is a schematic diagram of the integrated pump and valve control connection principle in an embodiment of the present invention.

[0058] Figure 4 This is a schematic diagram of the sampling box storage cabinet in an embodiment of the present invention. Detailed Implementation

[0059] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0060] According to the present invention, an integrated multi-stage sampling and mixing device includes: a sampling box cover component 1, a sampling box connector 2, a sampling unit 4, and a sample mixing unit; the sampling unit 4 includes: sampling components 401; the number of sampling components 401 is multiple; the sampling components 401 are evenly arranged at equal intervals in the sampling unit 4; the sample mixing unit is connected to the sampling unit 4; the sampling box cover component 1 is connected to the sampling unit 4 through the sampling box connector 2; the sampling box cover component 1 and the sampling box connector 2 are detachably connected; the sampling unit 4 and the sampling box connector 2 are detachably connected.

[0061] Specifically, in one embodiment, an integrated multi-channel pipette sample storage and mixing device uses a 12-channel multi-channel pipette sample storage rack for storing serum. Serum can be directly drawn from the storage rack during pipetting for sample addition; the integrated sample cap allows for individual capping and storage of the specimen in each well.

[0062] Preferably, the number of sampling components 401 is 96;

[0063] The sampling components 401 are arranged in 8 rows at equal intervals in the sampling unit 4.

[0064] Preferably, the sample mixing unit includes: a first sample mixing unit 5 and a second sample mixing unit 7;

[0065] The first sample mixing unit 5 and the second sample mixing unit 7 are respectively connected to the sampling unit 4.

[0066] Preferably, it further includes: a hybrid unit display component;

[0067] The mixing unit display component is connected to the sample mixing unit;

[0068] The mixing unit display component includes: a first sample mixing unit display component 6 and a second sample mixing unit display component 8;

[0069] The first sample mixing unit display component 6 is connected to the first sample mixing unit 5;

[0070] The second sample mixing unit display component 8 is connected to the second sample mixing unit 7.

[0071] Specifically, in one embodiment, information such as weight, capacity, and concentration in the mixing unit is sensed by a sensor.

[0072] Preferably, the sampling unit 4 further includes: a regulating valve 402 and a container connecting pipeline;

[0073] The sampling component 401 is a sampling container;

[0074] The container connecting pipes are connected to the sampling container and the sample mixing unit, respectively.

[0075] Preferably, the regulating valve 402 includes: a main regulating valve 411 and a branch regulating valve 412;

[0076] The container connection pipeline includes: a container connection branch pipeline 403 and a container connection main pipeline 404;

[0077] The main regulating valve is located on the main pipeline connecting the container;

[0078] The branch regulating valve is installed on the branch pipe connecting the container.

[0079] Preferably, it further includes: a liquid pump component 11;

[0080] The liquid pump component is connected to the connection point between the container connection main pipeline and the sample mixing unit.

[0081] Preferably, it further includes: a pump and valve integrated control unit 17, a strategy storage and calculation unit 16, and a user input unit 15;

[0082] The pump and valve integrated control component 17 is connected to the liquid pump component 11, the main regulating valve 411, and the branch regulating valve 412 respectively.

[0083] The pump and valve integrated control component 17 is connected to the strategy storage and calculation component 16;

[0084] The strategy storage and calculation unit 16 is connected to the user input unit 15.

[0085] Specifically, in one embodiment, an integrated multi-channel sample dispensing and mixing device can add a fixed amount of sample liquid each time, such as adding 100 μL of reagent and serum for mixing. This integrated multi-channel sample dispensing and mixing device ensures that the amount of liquid added from each sampling container is fixed. The integrated multi-channel sample dispensing and mixing device can also achieve a fixed amount of liquid added by mixing the liquid in the sample mixing unit into the sample dispensing tank.

[0086] Preferably, it also includes: a sampling box storage cabinet 14;

[0087] The sampling unit 4 also includes: a slide rail 3;

[0088] The slide rail 3 is located at the bottom of the sampling unit 4;

[0089] The sampling box storage cabinet 14 includes: a sampling box storage cabinet compartment, a sampling box storage cabinet control unit 142, and a compressor outdoor unit 143;

[0090] The inner wall of the sampling box storage cabinet is provided with a sliding groove;

[0091] The slide groove cooperates with the slide rail 3;

[0092] The sampling box storage cabinet control unit 142 can control any environmental variable inside the sampling box storage cabinet:

[0093] -Temperature variable;

[0094] -Gas pressure variables;

[0095] The sampling box storage cabinet control unit 142 is connected to the compressor outdoor unit 143.

[0096] Preferably, the sampling box storage cabinet 14 further includes: a sampling box storage cabinet display screen 144 and a sampling box storage cabinet recycling component 141;

[0097] The sampling box storage cabinet recycling component 141 is located at the lower part of the sampling box storage cabinet 14;

[0098] The display screen 144 of the sampling box storage cabinet is located on the outer wall of the sampling box storage cabinet 14.

[0099] Specifically, in one embodiment, the experimenter uses a sampling gun to sample reagents or serum into a sampling unit. The experimenter can set factors such as temperature and air pressure in the environment where the reagents or serum are located, as needed. The experimenter can place the sampling unit containing reagents or serum in a sampling box storage cabinet and view the temperature, air pressure, and other parameters of the environment where the sampling unit is located through a display device on the sampling box storage cabinet. The storage time of the sampling unit can also be timed. The temperature, air pressure, and other parameters of the environment where the sampling unit is located can be adjusted through a control device on the sampling box storage cabinet. The sampling unit is connected to a sample mixing unit. An intelligent control system controls the valves and liquid pumps on the connecting pipeline between the sampling unit and the sample mixing unit, thereby quantitatively extracting reagents or serum from the sampling unit into the sample mixing unit for mixing. In addition, the intelligent control system is connected to a strategy processing and analysis unit, thereby accurately quantitatively extracting reagents or serum from specific sampling components in the sampling unit into the sample mixing unit.

[0100] This invention employs a technical solution of equidistantly arranged sampling components, solving the problem of having to aspirate samples one by one from different serum ELISA plates, thus significantly reducing sample addition time. The invention has a reasonable structural design, is easy to use, and overcomes the shortcomings of existing technologies. It allows for very simple and convenient sample aspiration into the reaction container without causing secondary contamination, improving experimental efficiency. Furthermore, it allows for precise control of the amount of each sample added each time. It also allows for adjustment of environmental variables (such as temperature and air pressure) in the sampling box storage environment.

[0101] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0102] Specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. An integrated multi-stage sampling, preservation, and mixing device, characterized in that, include: Sampling box cover component (1), sampling box connector (2), sampling unit (4), sample mixing unit; The sampling unit (4) includes: a sampling component (401); The number of sampling components (401) is multiple; The sampling components (401) are evenly and equidistantly arranged in the sampling unit (4); The sample mixing unit is connected to the sampling unit (4); The sampling box cover component (1) is connected to the sampling unit (4) via the sampling box connector (2); The sampling box cover component (1) and the sampling box connector (2) are connected in a detachable manner; The sampling unit (4) and the sampling box connector (2) are connected in a detachable manner; The sampling unit (4) also includes: a regulating valve (402) and a container connection pipeline; The sampling component (401) is a sampling container; The container connecting pipes are respectively connected to the sampling container and the sample mixing unit; The regulating valve (402) includes: a main regulating valve (411) and a branch regulating valve (412); The container connection pipeline includes: a container connection branch pipeline (403) and a container connection main pipeline (404); The main regulating valve is located on the main pipeline connecting the container; The branch regulating valve is installed on the branch pipe connecting the container; It also includes: a liquid pump component (11); The liquid pump component is connected to the connection point between the container connection main pipeline and the sample mixing unit; Also includes: sampling box storage cabinet (14); The sampling unit (4) further includes: a slide rail (3); The slide rail (3) is located at the bottom of the sampling unit (4); The sampling box storage cabinet (14) includes: a sampling box storage cabinet compartment, a sampling box storage cabinet control unit (142), and a compressor outdoor unit (143); The inner wall of the sampling box storage cabinet is provided with a sliding groove; The groove is matched with the slide rail (3).

2. The integrated multi-stage sampling, preservation, and mixing device according to claim 1, characterized in that, The number of sampling components (401) is 96; The sampling components (401) are arranged in eight rows at equal intervals in the sampling unit (4).

3. The integrated multi-stage sampling, preservation, and mixing device according to claim 1, characterized in that, The sample mixing unit includes: a first sample mixing unit (5) and a second sample mixing unit (7); The first sample mixing unit (5) and the second sample mixing unit (7) are respectively connected to the sampling unit (4).

4. The integrated multi-stage sampling, preservation, and mixing device according to claim 3, characterized in that, It also includes: a hybrid unit display component; The mixing unit display component is connected to the sample mixing unit; The mixing unit display component includes: a first sample mixing unit display component (6) and a second sample mixing unit display component (8); The first sample mixing unit display component (6) is connected to the first sample mixing unit (5); The second sample mixing unit display component (8) is connected to the second sample mixing unit (7).

5. The integrated multi-stage sampling, preservation, and mixing device according to claim 1, characterized in that, Also includes: The pump and valve integrated control unit (17), the strategy storage and calculation unit (16), and the user input unit (15) are included. The pump and valve integrated control component (17) is connected to the liquid pump component (11), the main regulating valve (411), and the branch regulating valve (412) respectively; The pump and valve integrated control unit (17) is connected to the strategy storage and calculation unit (16); The strategy storage and operation unit (16) is connected to the user input unit (15).

6. The integrated multi-stage sampling, preservation, and mixing device according to claim 1, characterized in that, The sampling box storage cabinet control unit (142) can control any environmental variable inside the sampling box storage cabinet: -Temperature variable; -Gas pressure variables; The sampling box storage cabinet control unit (142) is connected to the compressor outdoor unit (143).

7. The integrated multi-stage sampling, preservation, and mixing device according to claim 1, characterized in that, The sampling box storage cabinet (14) also includes: a sampling box storage cabinet display screen (144) and a sampling box storage cabinet recycling component (141); The sampling box storage cabinet recycling component (141) is located at the lower part of the sampling box storage cabinet (14); The display screen (144) of the sampling box storage cabinet is located on the outer wall of the sampling box storage cabinet (14).

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