Substrate for tissue culture of dracocephalum rupestre hance as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of tissue culture, and particularly relates to a substrate for tissue culture of dracocephalum rupestre hance as well as a preparation method and application thereof. The substrate for tissue culture of dracocephalum rupestre hance is prepared from the following substances with the corresponding concentration: 0.2-0.5 mg / L of BA, 0.1-0.2 mg / L of IBA, 10-20g / L of cane sugar, 5-10g / L of eleusine indica powder, 5-10g / L of agar and a liquid culture medium with 1 / 2MS serving as a solvent. The 1 / 2MS liquid culture medium, the BA and the IBA are used for providing necessary growth factors and promotion factors for the growth of the tissue of the dracocephalum rupestre hance, and the eleusine indica powder and the corn stigma powder are used for improving the growth regulation performance of the BA and the IBA, so that the tissue of the dracocephalum rupestre hance grows fast, and the seedling rate is high.

Description

technical field

[0001] The invention belongs to the technical field of tissue culture, and in particular relates to a substrate for tissue culture of arborescens and its preparation method and application. Background technique

[0002] Maojiancao is a perennial herb, mainly distributed in Liaoning, Inner Mongolia, Hebei, Qinghai and other places. Maojiancao is a commonly used raw material of Chinese herbal medicine. The whole plant can be used as medicine. It can treat diseases such as gastroenteritis and nephritis, and has insecticidal effect. Studies have found that the main compounds in Maojiancao are protocatechuic acid, luteolin, flavonoids and other substances. However, due to the toxicity of Maojiancao, it is necessary to be cautious when using it. At present, most of the researches on Glycyrrhizae focus on the preparation of drugs, so the consumption of samples is relatively large, and the geographical distribution of wild Glycyrrhizae is limited, so artificial pla...

Examples

Embodiment 1

[0028] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.5mg / L, IBA0.2mg / L, sucrose 20g / L, goosegrass powder 5g / L, agar 5g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 200 mesh.

Embodiment 2

[0030] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.2mg / L, IBA0.1mg / L, sucrose 15g / L, goosegrass powder 10g / L, agar 10g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 100 mesh.

Embodiment 3

[0032] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.3mg / L, IBA0.15mg / L, sucrose 10g / L, goosegrass powder 8g / L, agar 8g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is goosegrass root powder dried to a moisture content of 3.8%, with a particle size of 200 mesh.

technical field

[0001] The invention belongs to the technical field of tissue culture, and in particular relates to a substrate for tissue culture of arborescens and its preparation method and application. Background technique

[0002] Maojiancao is a perennial herb, mainly distributed in Liaoning, Inner Mongolia, Hebei, Qinghai and other places. Maojiancao is a commonly used raw material of Chinese herbal medicine. The whole plant can be used as medicine. It can treat diseases such as gastroenteritis and nephritis, and has insecticidal effect. Studies have found that the main compounds in Maojiancao are protocatechuic acid, luteolin, flavonoids and other substances. However, due to the toxicity of Maojiancao, it is necessary to be cautious when using it. At present, most of the researches on Glycyrrhizae focus on the preparation of drugs, so the consumption of samples is relatively large, and the geographical distribution of wild Glycyrrhizae is limited, so artificial pla...

Examples

Embodiment 1

[0028] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.5mg / L, IBA0.2mg / L, sucrose 20g / L, goosegrass powder 5g / L, agar 5g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 200 mesh.

Embodiment 2

[0030] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.2mg / L, IBA0.1mg / L, sucrose 15g / L, goosegrass powder 10g / L, agar 10g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is the whole grass powder of goosegrass dried to a moisture content of 4.3%, with a particle size of 100 mesh.

Embodiment 3

[0032] A kind of substrate for tissue culture of hairy grass, made of the following concentrations of substances: BA 0.3mg / L, IBA0.15mg / L, sucrose 10g / L, goosegrass powder 8g / L, agar 8g / L, solvent is 1 / 2MS liquid medium. Goosegrass powder is goosegrass root powder dried to a moisture content of 3.8%, with a particle size of 200 mesh.

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