A device for enriching human feces-hatched miracidium

Abstract

The invention belongs to the technical field of schistosomiasis prevention and control, and discloses an enrichment device for hatching miracidia from human feces, comprising a liquid-imbibing graduated bottle, the top of which is threadedly connected to a graduated bottle upper cover, the top of which is fixedly connected to a No. 1 hose and a No. 2 hose, the other end of which is fixedly connected to a vacuum pump, the other end of which is fixedly connected to a liquid-imbibing top cover, and the bottom of which is threadedly connected to a bottom cover. The invention assembles a conical flask with a bottom cover and a sealing tube, and then uses a No. 2 hose and a No. 1 hose to respectively connect the liquid-imbibing graduated bottle with the bottom cover, and the liquid-imbibing graduated bottle with a vacuum pump, starts the vacuum pump, extracts the gas inside the graduated bottle upper cover, and makes the inside of the liquid-imbibing graduated bottle present a partial vacuum state, the partial vacuum is transmitted to the inside of the bottom cover through the No. 2 hose, and the conical liquid-imbibing head inside the bottom cover adsorbs the liquid in the conical flask and miracidia together on an enrichment filter membrane.

Description

Technical Field

[0001] The invention belongs to the technical field of schistosomiasis prevention and treatment, and in particular is a device for enriching miracidia hatched from human feces. Background Art

[0002] Schistosomiasis japonicum is a zoonosis that seriously affects the health of infected people and brings about major social and economic impacts. The gold standard for diagnosing schistosomiasis is to find eggs or hatched miracidia in the feces of infected people. The current epidemic situation of schistosomiasis is mainly low infection. It is difficult to find schistosome eggs by microscopic examination through the modified Kato thick smear method (41.7 mg / piece). The hatching method (human feces 30g) can greatly improve the detection rate of schistosomiasis because of the large amount of feces examined. The fecal hatching method mainly uses a conical flask, and the hatched miracidia are active in the neck of the conical flask. The inspection of fecal hatching miracidia is done by the naked eye or a magnifying glass on the neck of the conical flask, which depends on personal experience. Due to the refractive index of the glass conical flask, the turbidity of the hatched fecal liquid, the small number of miracidia or the poor vitality, it is easy to cause missed detection and misdetection, which affects the diagnosis and prevention of schistosomiasis.

[0003] The enrichment device for miracidia hatched from human feces enriches the miracidia hatched in the bottleneck of a triangular flask through an enrichment probe, thereby achieving the purpose of being convenient and fast and improving the detection rate. The method adopted in the present invention has high requirements on the air tightness of the device, so the present invention proposes a new solution to the air tightness. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems. The present invention provides an enrichment device for hatching miracidia from human feces, which has the advantages of preventing missed detection and false detection and strong air tightness.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an enrichment device for hatching miracidium from human feces, comprising a liquid-imbibing graduated bottle, the top of the liquid-imbibing graduated bottle being threadedly connected to a graduated bottle upper cover, the top of the graduated bottle upper cover being fixedly connected to a No. 1 hose and a No. 2 hose, the other end of the No. 1 hose being fixedly connected to a vacuum pump, the other end of the No. 2 hose being fixedly connected to a liquid-imbibing top cover, the liquid-imbibing top cover being threadedly connected to a bottom cover, the interior of the bottom cover being fixedly sleeved with a sealing tube, the interior of the bottom cover being provided with an enrichment filter membrane, a protective net and a conical liquid-imbibing head, the enrichment filter membrane, the protective net and the conical liquid-imbibing head The head is fixedly connected to the bottom cover through the top cover of the pipette and is clamped inside the bottom cover. The protective net is placed above the enrichment filter membrane, and the bottom of the enrichment filter membrane and the top of the protective net are in contact with the conical pipette head and the bottom cover respectively. The protective net plays a role of isolating and supporting the enrichment filter membrane, so that the miracidia adhere to the filter membrane without tearing the enrichment filter membrane, and the filtrate can quickly pass through the filter membrane along the No. 2 hose into the pipette bottle. The outer edge of the conical pipette head is in squeeze contact with the top of the sealing tube, and the inner side of the sealing tube is movably clamped with a conical flask, and the bottom end port of the conical pipette head extends downward to the neck of the conical flask.

[0006] As a preferred technical solution of the present invention, the bottom of the graduated bottle upper cover is provided with an upper cover extension edge, the top of the liquid-absorbing graduated bottle is provided with a graduated bottle extension edge, the top surface of the graduated bottle extension edge is provided with an annular groove, a sealing ring is fixedly clamped in the annular groove, and the sealing ring is in contact with the upper cover extension edge.

[0007] As a preferred technical solution of the present invention, the top of the outer side surface of the liquid-absorbing scale bottle is provided with an external thread, the bottom of the inner side surface of the scale bottle upper cover is provided with an internal thread, the external thread of the liquid-absorbing scale bottle is adapted to the internal thread of the scale bottle upper cover, the bottom end of the liquid-absorbing top cover is provided with an external thread, the inner side of the bottom of the bottom cover is provided with an internal thread, the external thread of the liquid-absorbing top cover is adapted to the internal thread of the bottom cover, and the present invention adopts an assembled combined structure, which is convenient for installation and disassembly, and is easy to clean and maintain.

[0008] As a preferred technical solution of the present invention, the bottom cover is provided with a circular through hole, and the sealing tube, enrichment filter membrane, protective net and conical liquid pipette head are all located in the circular through hole, and the outer diameters of the sealing tube, enrichment filter membrane and protective net are adapted to the diameter of the circular through hole.

[0009] As a preferred technical solution of the present invention, the sealing tube is divided into two parts, a top section and a middle and lower section. The inner diameter of the top section of the sealing tube is larger than the inner diameter of the middle and lower section. The connecting part between the inner top section and the middle and lower section of the sealing tube is an arc surface. The inner arc surface of the sealing tube fits with the top end port of the conical flask. During assembly, after the top end of the conical flask is fully fitted with the arc surface inside the sealing tube, the contact area between the inner wall of the sealing tube and the conical flask is increased, thereby improving the air tightness. In addition, since the inner diameter of the top end of the sealing tube is smaller than that of the port of the conical flask, it also plays a limiting role. When the sealing tube and the conical flask are assembled, the conical flask will not pass through the top end of the sealing tube.

[0010] As a preferred technical solution of the present invention, a raised portion with an arc-shaped cross-section is provided at the bottom of the outer extension edge of the upper cover, and the raised portion is in close contact with the sealing ring and presses the outer extension edge of the scale bottle concavely. The raised portion also presents a circular ring shape. When the upper cover of the scale bottle is threadedly connected to the liquid-absorbing scale bottle and tightened downward, the raised portion presses the sealing ring downward and presses the sealing ring concavely. The surface of the raised portion is frosted and rough in texture, so it can fit more closely with the sealing ring and have a better sealing effect.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] 1. The present invention assembles a conical flask with a bottom cover and a sealing tube, and then uses a No. 2 hose and a No. 1 hose to respectively connect a liquid-imbibing scale bottle with the bottom cover, and a liquid-imbibing scale bottle with a vacuum pump, and starts the vacuum pump to extract the gas inside the upper cover of the scale bottle, so that the inside of the liquid-imbibing scale bottle is in a partial vacuum state, and the partial vacuum is transmitted to the inside of the bottom cover through the No. 2 hose. The conical liquid-imbibing head in the bottom cover adsorbs the liquid and miracidia in the conical flask on the enrichment filter membrane, and the enrichment filter membrane is subjected to a microscope inspection, so that a qualitative inspection can be quickly carried out, and the observation is more comprehensive, thereby eliminating the situation of missed detection and false detection.

[0013] 2. The present invention improves the air tightness by making the top of the conical flask fit fully with the arc surface inside the sealing tube, thereby increasing the contact area between the inner wall of the sealing tube and the conical flask. The bottom of the outer edge of the upper cover is provided with a convex portion with an arc-shaped cross-section. The convex portion is in close contact with the sealing ring and presses the outer edge of the graduated bottle into a concave shape. The surface of the convex portion is frosted and rough in texture, so it can fit more closely with the sealing ring and has a better sealing effect. By improving the air tightness, the inside of the device can reach the working conditions more quickly, the enrichment speed is accelerated, and the work efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the present invention;

[0015] Figure 2 It is an exploded schematic diagram of the liquid-imbibing graduated bottle and the graduated bottle upper cover of the present invention;

[0016] Figure 3 It is a cross-sectional view of the liquid-absorbing graduated bottle and the graduated bottle upper cover of the present invention;

[0017] Figure 4 It is a schematic diagram of the internal decomposition of the bottom cover of the present invention;

[0018] Figure 5 It is a cross-sectional view of the internal structure of the bottom cover of the present invention.

[0019] In the figure: 1. Pipette scale bottle; 2. Scale bottle upper cover; 3. Outer edge of upper cover; 4. Outer edge of scale bottle; 5. Sealing ring; 6. No. 1 hose; 7. Vacuum pump; 8. No. 2 hose; 9. Bottom cover; 10. Pipette top cover; 11. Sealing tube; 12. Enrichment filter membrane; 13. Protective net; 14. Erlenmeyer flask; 15. Conical pipette head. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] like Figures 1 to 5 As shown, the present invention provides an enrichment device for hatching miracidium from human feces, comprising a liquid-imbibing graduated bottle, the top of which is threadedly connected to a graduated bottle upper cover, a No. 1 hose and a No. 2 hose fixedly connected to the top of the graduated bottle upper cover, the other end of the No. 1 hose fixedly connected to a vacuum pump, the other end of the No. 2 hose fixedly connected to a liquid-imbibing top cover, the liquid-imbibing top cover is threadedly connected to a bottom cover, a sealing tube is fixedly sleeved inside the bottom cover, an enrichment filter membrane, a protective net and a conical liquid-imbibing head are arranged inside the bottom cover, the enrichment filter membrane, the protective net and the conical liquid-imbibing head are threadedly connected to the liquid-imbibing top cover, It is connected to the bottom cover and fixedly clamped inside the bottom cover, the protective net is placed above the enrichment filter membrane and the bottom of the enrichment filter membrane and the top of the protective net are in contact with the conical pipette head and the bottom cover respectively, the protective net plays a role of isolating and supporting the enrichment filter membrane here, so that the miracidia adhere to the filter membrane without tearing the enrichment filter membrane, and the filtrate can quickly pass through the filter membrane along the No. 2 hose into the pipette bottle, the outer edge of the conical pipette head is in squeeze contact with the top of the sealing tube, the inner side of the sealing tube is movably clamped with a conical flask, and the bottom end port of the conical pipette head extends downward to the neck of the conical flask.

[0022] Among them, the bottom of the graduated bottle upper cover 2 is provided with an upper cover extension edge 3, the top of the liquid-absorbing graduated bottle 1 is provided with a graduated bottle extension edge 4, the top surface of the graduated bottle extension edge 4 is provided with an annular groove, and a sealing ring 5 is fixedly clamped in the annular groove, and the sealing ring 5 is in contact with the upper cover extension edge 3.

[0023] Among them, an external thread is provided on the top of the outer side of the liquid-imbibing scale bottle 1, and an internal thread is provided on the bottom of the inner side of the scale bottle upper cover 2. The external thread of the liquid-imbibing scale bottle 1 is adapted to the internal thread of the scale bottle upper cover 2. The bottom end of the liquid-imbibing top cover 10 is provided with an external thread, and the inner side of the bottom of the bottom cover 9 is provided with an internal thread. The external thread of the liquid-imbibing top cover 10 is adapted to the internal thread of the bottom cover 9. The present invention adopts an assembled combined structure, which is easy to install and disassemble, and is convenient for cleaning and maintenance.

[0024] The bottom cover 9 is provided with a circular through hole, in which the sealing tube 11, the enrichment filter membrane 12, the protection net 13 and the conical liquid pipette head 15 are all located. The outer diameters of the sealing tube 11, the enrichment filter membrane 12 and the protection net 13 match the diameter of the circular through hole.

[0025] Among them, the sealing tube 11 is divided into two parts, a top section and a middle and lower section. The inner diameter of the top section of the sealing tube 11 is larger than the inner diameter of the middle and lower section. The connecting part between the inner top section and the middle and lower section of the sealing tube 11 is an arc surface. The inner arc surface of the sealing tube 11 fits with the top end port of the conical flask 14. During assembly, after the top end of the conical flask 14 is fully fitted with the arc surface inside the sealing tube 11, the contact area between the inner wall of the sealing tube 11 and the conical flask 14 is increased, thereby improving the air tightness. In addition, since the inner diameter of the top end of the sealing tube 11 is smaller than that of the port of the conical flask 14, it also plays a limiting role. When the sealing tube 11 and the conical flask 14 are assembled, the conical flask 14 will not pass through the top end of the sealing tube 11.

[0026] Among them, a convex part with an arc-shaped cross-section is provided at the bottom of the outer extension edge 3 of the upper cover, the convex part is in close contact with the sealing ring 5 and presses the outer extension edge 4 of the scale bottle into a concave shape, and the convex part also presents a circular ring shape. When the scale bottle upper cover 2 is threadedly connected with the liquid-absorbing scale bottle 1 and tightened downward, the convex part presses the sealing ring 5 downward and presses the sealing ring 5 into a concave shape. The surface of the convex part is frosted and rough in texture, so that it can fit more closely with the sealing ring 5 and have a better sealing effect.

[0027] The working principle and use process of the present invention:

[0028] A conical flask is used as a container for feces incubation. The hatched miracidia gather at the neck of the conical flask 14. The conical flask 14 is assembled with the bottom cover 9 and the sealing tube 11. After the assembly is completed, the air tightness of the device is checked. Then, the No. 2 hose 8 and the No. 1 hose 6 are used to connect the liquid-pipetting scale bottle 1 and the bottom cover 9, and the liquid-pipetting scale bottle 1 and the vacuum pump 7 respectively. The vacuum pump 7 is started and the vacuum pump 7 is used to evacuate air to extract the gas inside the scale bottle upper cover 2, so that the interior of the liquid-pipetting scale bottle 1 is in a partial vacuum state. The partial vacuum is transmitted to the interior of the bottom cover 9 through the No. 2 hose 8. The conical liquid-pipetting head 15 can adsorb the liquid in the neck of the conical flask 14 and the miracidia gathered thereon onto the enrichment filter membrane 12. The enrichment filter membrane 12 is examined under a microscope, and a qualitative inspection can be performed quickly.

[0029] During assembly, after the top of the conical flask 14 is fully fitted with the arc surface inside the sealing tube 11, the contact area between the inner wall of the sealing tube 11 and the conical flask 14 is increased, thereby improving the air tightness. A convex portion with an arc-shaped cross-section is provided at the bottom of the outer extension edge 3 of the upper cover. The convex portion is in close contact with the sealing ring 5 and concave the outer extension edge 4 of the scale bottle. The convex portion also presents a circular ring shape. When the scale bottle upper cover 2 is threadedly connected with the liquid-absorbing scale bottle 1 and tightened downward, the convex portion presses the sealing ring 5 downward and concave the sealing ring 5. The surface of the convex portion is frosted and rough in texture, so it can fit more closely with the sealing ring 5 and have a better sealing effect.

[0030] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0031] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for enriching human feces hatched miracidium, comprising a liquid pipetting graduated bottle (1), characterized in that: The top of the liquid-imbibing graduated bottle (1) is threadedly connected to a graduated bottle upper cover (2); the top of the graduated bottle upper cover (2) is fixedly connected to a No. 1 hose (6) and a No. 2 hose (8); the other end of the No. 1 hose (6) is fixedly connected to a vacuum pump (7); the other end of the No. 2 hose (8) is fixedly connected to a liquid-imbibing top cover (10); the liquid-imbibing top cover (10) is threadedly connected to a bottom cover (9); a sealing tube (11) is fixedly sleeved inside the bottom cover (9); an enrichment filter membrane (12), a protective net (13) and a conical liquid-imbibing head (15) are arranged inside the bottom cover (9); the enrichment filter membrane (12), The protective net (13) and the conical liquid pipetting head (15) are fixedly clamped inside the bottom cover (9) by means of a liquid pipetting top cover (10) being threadedly connected to the bottom cover (9); the protective net (13) is placed above the enrichment filter membrane (12), and the bottom of the enrichment filter membrane (12) and the top of the protective net (13) are in contact with the conical liquid pipetting head (15) and the bottom cover (9) respectively; the outer edge of the conical liquid pipetting head (15) is in compression contact with the top of the sealing tube (11); the inner side of the sealing tube (11) is movably clamped with a conical flask (14); and the bottom end port of the conical liquid pipetting head (15) extends downward to the neck of the conical flask (14).

2. The device for enriching human feces-hatched miracidia according to claim 1, characterized in that: The bottom of the graduated bottle upper cover (2) is provided with an upper cover outer edge (3), the top of the liquid-absorbing graduated bottle (1) is provided with a graduated bottle outer edge (4), the top surface of the graduated bottle outer edge (4) is provided with an annular groove, a sealing ring (5) is fixedly clamped in the annular groove, and the sealing ring (5) is in contact with the upper cover outer edge (3).

3. The device for enriching human feces-hatched miracidia according to claim 1, characterized in that: The top of the outer side of the liquid-absorbing scale bottle (1) is provided with an external thread, the bottom of the inner side of the scale bottle upper cover (2) is provided with an internal thread, the external thread of the liquid-absorbing scale bottle (1) is adapted to the internal thread of the scale bottle upper cover (2), the bottom end of the liquid-absorbing top cover (10) is provided with an external thread, the inner side of the bottom of the bottom cover (9) is provided with an internal thread, the external thread of the liquid-absorbing top cover (10) is adapted to the internal thread of the bottom cover (9).

4. The device for enriching human feces-hatched miracidia according to claim 1, characterized in that: The bottom cover (9) is provided with a circular through hole, and the sealing tube (11), the enrichment filter membrane (12), the protective net (13) and the conical liquid pipetting head (15) are all located in the circular through hole, and the outer diameters of the sealing tube (11), the enrichment filter membrane (12) and the protective net (13) are adapted to the diameter of the circular through hole.

5. The device for enriching human feces-hatched miracidia according to claim 1, characterized in that: The sealing tube (11) is divided into two parts, a top section and a middle and lower section. The inner diameter of the top section of the sealing tube (11) is larger than the inner diameter of the middle and lower section. The connecting part between the top section and the middle and lower section of the sealing tube (11) is a curved surface. The inner curved surface of the sealing tube (11) fits the top end of the conical flask (14).

6. The device for enriching human feces hatched miracidia according to claim 2, characterized in that: A raised portion with an arc-shaped cross section is provided at the bottom of the outer extension edge (3) of the upper cover, and the raised portion is in close contact with the sealing ring (5) and presses the outer extension edge (4) of the graduated bottle into a concave shape.

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