A gas internal circulation algae photosynthetic oxygen production test device

Abstract

The present invention discloses a gas internal circulation type algae photosynthetic oxygen production testing device, which relates to the technical field of algae oxygen production monitoring, and includes a culture structure and a control system. The culture structure is used to hold algae, and an aeration structure is provided in the culture structure. One end of the aeration structure is connected to the opening of the culture structure through a first pipeline, and an oxygen detector is provided on the first pipeline, and the oxygen detector is electrically connected to the control system. When the present invention is used, the algae and the nutrient solution are placed in the culture structure, and the generated oxygen flows back to the aeration structure through the first pipeline, and enters the culture structure through the aeration structure to stir the algae and the nutrient solution. The oxygen detector on the first pipeline monitors the oxygen concentration in real time, and the reading of the oxygen detector can be directly read and displayed in real time. It is simple to operate and can be updated in real time, and can accurately reflect the process of algae photosynthetic oxygen production.

Description

Technical Field

[0001] The invention relates to the technical field of algae oxygen production monitoring, in particular to a gas internal circulation type algae photosynthetic oxygen production testing device. Background Art

[0002] With the development of society and the progress of mankind, in terms of the way of increasing oxygen in limited space environments such as national defense, military industry, medical health, etc., humans are increasingly pursuing green and sustainable development. Microalgae, as an efficient biological photosynthetic oxygen production method, is in line with the development trend of mankind. In addition to its extremely high photosynthetic efficiency, microalgae also has many advantages such as strong adaptability, short growth cycle, and continuous cultivation. Therefore, it has wide applicability and economy.

[0003] While solving the problem of how to produce oxygen, how to simply, efficiently, stably and scientifically evaluate the photosynthetic oxygen production efficiency of microalgae is also a problem we must face. However, there are few reports on integrated devices and methods for the photosynthetic oxygen production efficiency of microalgae. At present, most of them only know that oxygen can be produced, but the specific oxygen production rate is unknown. Therefore, we urgently hope that there can be standardized monitoring methods and system devices to perform relevant measurements.

[0004] There are two traditional methods for monitoring photosynthetic oxygen production of microalgae:

[0005] 1. Chlorophyll fluorescence method: Chlorophyll fluorescence measurement reflects the state of microalgae chloroplasts and indirectly reflects the photosynthesis efficiency of plants. Specifically, the plants are treated in the dark to keep the microalgae chloroplasts in the ground state, and then a strong beam of light or pulsed light is irradiated in a very short time (the time can be set, generally about 15 milliseconds). The machine will collect relevant light absorption and other parameters. FluorCams or similar equipment are required;

[0006] 2. Gas chromatography: Collect the generated gas in a closed container using the drainage method or other methods, and then monitor it using equipment such as gas chromatography.

[0007] The above two methods have the following defects:

[0008] 1) Both chlorophyll fluorescence method and gas chromatography method have the problem of relying on expensive detection equipment, complicated operation and inability to read monitoring results in real time;

[0009] 2) The chlorophyll fluorescence method is an indirect method to obtain photosynthesis efficiency. It cannot be converted into the actual amount of oxygen produced. It can only be used for relative speed comparison and has low accuracy.

[0010] 3) The chlorophyll fluorescence method requires sampling of the algae solution first, and dark treatment for 2 hours before monitoring and obtaining results, which cannot reflect real-time results. Summary of the invention

[0011] The purpose of the present invention is to provide a gas internal circulation algae photosynthetic oxygen production testing device to solve the problems existing in the above-mentioned prior art, monitor the oxygen concentration in real time, and the operation is simple, and the process of algae photosynthetic oxygen production can be accurately reflected.

[0012] To achieve the above object, the present invention provides the following solutions:

[0013] The present invention provides a gas internal circulation algae photosynthetic oxygen production testing device, comprising a culture structure and a control system, wherein the culture structure is used to hold algae, an aeration structure is arranged in the culture structure, one end of the aeration structure is connected to an opening of the culture structure through a first pipeline, an oxygen detector is arranged on the first pipeline, and the oxygen detector is electrically connected to the control system.

[0014] Preferably, a lighting device is provided on the outside of the culture structure, the lighting device is electrically connected to the control system, and the lighting device is used to illuminate the algae in the culture structure.

[0015] Preferably, a detection probe group is provided in the culture structure, and the detection probe group is used to monitor pH, dissolved oxygen content and algae liquid concentration, and the detection probe group is electrically connected to the control system.

[0016] Preferably, a first valve and a second valve are provided on the first pipeline, the first pipeline on one side of the first valve is connected to one end of the second pipeline, the first pipeline on one side of the second valve is connected to one end of the third pipeline, the other end of the second pipeline and the other end of the third pipeline are both connected to the outside atmosphere, the third valve is provided on the second pipeline, and the fourth valve is provided on the third pipeline, and the first valve, the second valve, the third valve and the fourth valve are all electrically connected to the control system.

[0017] Preferably, a gas buffer tank is provided on the first pipeline, two ends of the gas buffer tank are connected with two ends of the fourth pipeline, the oxygen detector is provided on the fourth pipeline, and a gas dryer is also provided on the fourth pipeline, and the gas dryer is provided upstream of the oxygen detector.

[0018] Preferably, an air pressure sensor is provided on the gas buffer tank, and the air pressure sensor is electrically connected to the control system.

[0019] Preferably, the gas internal circulation algae photosynthetic oxygen production testing device also includes a cooling structure, the cooling structure includes a cooler, the cooler is electrically connected to the control system, a cooling pipe is arranged in the culture structure, one end of the cooler is connected to one end of the cooling pipe through a fifth pipe, and the other end of the cooler is connected to the other end of the cooling pipe through a sixth pipe.

[0020] Preferably, the gas internal circulation type algae photosynthetic oxygen production testing device further comprises a feeding structure, and the feeding structure is connected to the culture structure via a seventh pipeline.

[0021] Preferably, a feed pump is provided on the seventh pipeline, and the feed pump is electrically connected to the control system.

[0022] Preferably, a gas circulation pump is provided on the first pipeline, and the gas circulation pump is electrically connected to the control system.

[0023] Compared with the prior art, the present invention has achieved the following technical effects:

[0024] When the present invention is used, algae and nutrient solution are placed in the culture structure, and the generated oxygen flows back to the aeration structure through the first pipeline, and enters the culture structure through the aeration structure to stir the algae and the nutrient solution. The oxygen detector on the first pipeline monitors the oxygen concentration in real time, and the reading of the oxygen detector can be directly read and displayed in real time. It is easy to operate and can be updated in real time, and can accurately reflect the process of photosynthetic oxygen production of algae. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 It is a schematic diagram of the gas internal circulation type algae photosynthetic oxygen production testing device of the present invention;

[0027] Figure 2 It is a control logic schematic diagram of the present invention;

[0028] Among them: 100-gas internal circulation algae photosynthetic oxygen production test device; 1-signal connecting line; 2-first pipeline; 3-cooling structure; 4-illumination device; 5-cultivation structure; 6-aeration structure; 7-cooling pipe; 8-cooling machine; 9-detection probe group; 10-first valve; 11-third valve; 12-second valve; 13-fourth valve; 14-gas circulation pump; 15-gas buffer tank; 16-air pressure sensor; 17-gas dryer; 18-oxygen detector; 19-control system; 20-relay; 21-feeding pump; 22-feeding structure; 23-second pipeline; 24-third pipeline; 25-fourth pipeline; 26-fifth pipeline; 27-sixth pipeline; 28-seventh pipeline. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The purpose of the present invention is to provide a gas internal circulation algae photosynthetic oxygen production testing device to solve the problems existing in the above-mentioned prior art, monitor the oxygen concentration in real time, and the operation is simple, and the process of algae photosynthetic oxygen production can be accurately reflected.

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] Embodiment 1

[0033] like Figure 1-Figure 2 As shown: This embodiment provides a gas internal circulation algae photosynthetic oxygen production test device 100, including a culture structure 5 and a control system 19. The culture structure 5 is used to hold algae. This embodiment uses microalgae. An aeration structure 6 is provided in the culture structure 5. One end of the aeration structure 6 is connected to the upper end opening of the culture structure 5 through a first pipeline 2. An oxygen detector 18 is provided on the first pipeline 2. The oxygen detector 18 is electrically connected to the control system 19. When this embodiment is used, the algae and the nutrient solution are placed in the culture structure 5, and the generated oxygen flows back to the aeration structure 6 through the first pipeline 2, and enters the culture structure 5 through the aeration structure 6 to stir the algae and the nutrient solution. The oxygen detector 18 on the first pipeline 2 monitors the oxygen concentration in real time, and the reading of the oxygen detector 18 can be directly read and displayed in real time. It is simple to operate and can be updated in real time, and can accurately reflect the process of algae photosynthetic oxygen production.

[0034] Specifically, in this embodiment, the culture structure 5 is composed of a plurality of acrylic culture plates.

[0035] In this embodiment, an illumination device 4 is provided on the outside of the culture structure 5 . The illumination device 4 is an adjustable LED uniform light flat panel lamp. The light source control module of the illumination device 4 is electrically connected to the control system 19 . The illumination device 4 is used to illuminate the algae in the culture structure 5 .

[0036] In this embodiment, the aeration structure 6 is a U-shaped PVC aeration pipe, a plurality of air holes are arranged at the bottom of the U-shaped PVC aeration pipe, the bottom of the U-shaped PVC aeration pipe is located in the algae liquid composed of algae and nutrient solution, one end of the first pipeline 2 is connected to one end of the U-shaped PVC aeration pipe, and the U-shaped PVC aeration pipe stirs the algae liquid by releasing bubbles.

[0037] In this embodiment, a detection probe group 9 is provided in the culture structure 5, and the detection probe group 9 is used to monitor pH, dissolved oxygen content and algae liquid concentration, and the detection probe group 9 is electrically connected to the control system 19. The detection probe group 9 includes a pH probe, a DO probe and a chlorophyll monitoring probe (CHL. probe). The pH probe is used to monitor the pH of the algae liquid, the DO probe is used to monitor the dissolved oxygen content of the algae liquid, and the chlorophyll monitoring probe is used to monitor the concentration of the algae liquid.

[0038] In this embodiment, the first pipeline 2 is provided with a first valve 10 and a second valve 12, the first pipeline 2 on one side of the first valve 10 is connected to one end of the second pipeline 23, the first pipeline 2 on one side of the second valve 12 is connected to one end of the third pipeline 24, the other end of the second pipeline 23 and the other end of the third pipeline 24 are both connected to the outside atmosphere, the second pipeline 23 is provided with a third valve 11, the third pipeline 24 is provided with a fourth valve 13, and the first valve 10, the second valve 12, the third valve 11 and the fourth valve 13 are all electrically connected to the control system 19. In this embodiment, the first valve 10, the second valve 12, the third valve 11 and the fourth valve 13 are all two-position two-way gas solenoid valves. The first valve 10, the second valve 12, the third valve 11 and the fourth valve 13 control the gas flow path to maintain the oxygen partial pressure concentration and gas pressure in the device.

[0039] In this embodiment, a gas buffer tank 15 is provided on the first pipeline 2, and both ends of the gas buffer tank 15 are connected to both ends of the fourth pipeline 25. An oxygen detector 18 is provided on the fourth pipeline 25, and a gas dryer 17 is also provided on the fourth pipeline 25. The gas dryer 17 is provided upstream of the oxygen detector 18. An air suction pump is provided inside the oxygen detector 18, and the gas in the gas buffer tank 15 is dried by the gas dryer 17 and then introduced into the oxygen detector 18. The oxygen detector 18 monitors the oxygen component in the gas, and the control system 19 displays real-time data through software and controls the working status of each structure.

[0040] In this embodiment, a gas pressure sensor 16 is provided on the gas buffer tank 15. The gas pressure sensor 16 is electrically connected to the control system 19. The gas pressure sensor 16 is used to monitor the pressure in the device and further control the pressure range.

[0041] In this embodiment, the gas internal circulation algae photosynthetic oxygen production test device 100 also includes a cooling structure 3, the cooling structure 3 includes a cooler 8, the cooler 8 in this embodiment uses cooling water for cooling, the cooler 8 is electrically connected to the control system 19, a cooling pipe 7 is provided in the culture structure 5, the cooling pipe 7 is a stainless steel pipe, one end of the cooler 8 is connected to one end of the cooling pipe 7 through a fifth pipeline 26, and the other end of the cooler 8 is connected to the other end of the cooling pipe 7 through a sixth pipeline 27. The cooling structure 3 maintains the algae liquid in the culture structure 5 at 25°C.

[0042] In this embodiment, the gas internal circulation algae photosynthetic oxygen production test device 100 further includes a feeding structure 22, which is a feeding bottle, and NaHCO3 is contained in the feeding structure 22. The feeding structure 22 is connected to the culture structure 5 through the seventh pipeline 28. The feeding structure 22 is used to maintain a stable pH of the algae solution and add a carbon source required for photosynthesis.

[0043] In this embodiment, a feed pump 21 is provided on the seventh pipeline 28 , and the feed pump 21 is electrically connected to the control system 19 .

[0044] In this embodiment, a gas circulation pump 14 is provided on the first pipeline 2. The gas circulation pump 14 is electrically connected to the control system 19. The gas circulation pump 14 provides circulation power for the gas to circulate inside the system.

[0045] In this embodiment, the relay 20 controls the on / off status of the first valve 10 , the second valve 12 , the third valve 11 , the fourth valve 13 , the cooling structure 3 , the feed pump 21 and the gas circulation pump 14 , and the relay 20 is a multi-channel relay.

[0046] This embodiment aims at structural improvement, and the control process of the control system 19 is the existing technology.

[0047] When oxygen monitoring is performed using this embodiment, the control system 19 controls the first valve 10 and the second valve 12 to open, the third valve 11 and the fourth valve 13 to close through the relay 20. Under the push of the gas circulation pump 14, the gas in the top space of the culture structure 5 enters the first pipeline 2, and is introduced into the aeration structure 6 after passing through the first valve 10, the second valve 12, the gas circulation pump 14, and the gas buffer tank 15 in sequence, and finally released into the algae liquid through the pores at the bottom of the aeration structure 6, that is, it returns to the top space of the culture structure 5 to form a closed loop. The oxygen detector 18 uses an internal suction pump to dry the gas in the gas buffer tank 15 through the gas dryer 17, and then introduces it into the oxygen detector 18 for monitoring. Then the gas flows back into the gas buffer tank 15 to form a loop, and the oxygen monitoring results are uploaded to the control system 19 through the signal connection line 1. The control system 19 displays the results in real time through the internal program. During the normal operation of the device of this embodiment, the control system 19 makes necessary adjustments to the photosynthetic oxygen production process of the microalgae through the data monitored by the oxygen detector 18 to ensure safety and maximize oxygen production efficiency.

[0048] When the oxygen feedback regulation is performed using this embodiment, in order to prevent oxygen accumulation from causing excessive oxygen partial pressure in the device to inhibit the photosynthesis efficiency of microalgae, when the oxygen concentration monitored by the oxygen detector 18 reaches a set value (which can be set in the software interface of the control system 19 according to actual conditions, for example, oxygen concentration ≤ 50%), the control system 19 controls the first valve 10 and the second valve 12 to close, and the third valve 11 and the fourth valve 13 to open through the relay 20, thereby changing the path of gas circulation, that is, under the promotion of the gas circulation pump 14, the gas in the atmosphere passes through the fourth valve 13, the gas circulation pump 14, and the gas buffer tank 15 in sequence, and then enters the aeration structure 6, that is, enters the top space of the culture structure 5, and finally returns to the atmosphere from the third valve 11, until the value measured by the oxygen detector 18 is 20.9% ± 0.1, the gas replacement is completed, and a new round of monitoring can be carried out.

[0049] When the present embodiment is used for air pressure feedback regulation, in order to prevent excessive air pressure inside the closed device due to oxygen production or other erroneous operations, thereby causing damage such as explosion to various components of the device, when the pressure monitored by the air pressure sensor 16 exceeds the set value of 0.01Mpa, the control system 19 will control each component to execute the above-mentioned gas replacement procedure.

[0050] When the pH feedback regulation is performed using this embodiment, in order to prevent the decrease of the photosynthetic efficiency of the microalgae due to the increase of pH or the insufficient supply of carbon source, when the result monitored by the pH probe exceeds the set range of 7 to 7.5 (which can be set according to the actual situation), the control system 19 controls the working state of the feed pump 21 through the relay 20, replenishes NaHCO3, adjusts the pH, and replenishes the carbon source;

[0051] When the temperature feedback regulation is performed using this embodiment, in order to prevent the photosynthetic efficiency of the microalgae from decreasing due to the temperature rise, the control system 19 controls the working state of the cooling machine 8 through the relay 20 according to the comparison between the actual monitoring value and the set range.

[0052] The gas internal circulation algae photosynthetic oxygen production test device 100 of the present embodiment is a closed monitoring structure; the present embodiment adopts an oxygen partial pressure control mechanism to automatically monitor the oxygen content at the top of the culture structure 5 to prevent excessive oxygen partial pressure from causing a decrease in photosynthesis; gas replacement is achieved by opening and closing the first valve 10, the second valve 12, the third valve 11 and the fourth valve 13 in two ways; air pressure feedback regulation is used to prevent equipment accidents or accidents caused by excessive air pressure in the closed device; pH feedback regulation is used to prevent the decrease in microalgae photosynthetic efficiency due to increased pH or insufficient carbon source supply.

[0053] Embodiment 2

[0054] The difference between this embodiment and the first embodiment is that: the first pipeline 2 is provided with a first valve 10 and a second valve 12, both of which are two-position three-way gas solenoid valves, and both of which include a common port and two non-common ports, and the common ports of the first valve 10 and the second valve 12 are both connected to one end of the first pipeline 2, and one non-common port of the first valve 10 and the second valve 12 is connected to the other end of the first pipeline 2, and the other non-common ports of the first valve 10 and the second valve 12 are both connected to the outside atmosphere. In this embodiment, the third valve 11, the fourth valve 13, the second pipeline 23 and the third pipeline 24 do not need to be provided.

[0055] The present specification uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A gas internal circulation algae photosynthetic oxygen production test device, characterized in that: It comprises a culture structure and a control system, wherein the culture structure is used to hold algae, an aeration structure is arranged in the culture structure, one end of the aeration structure is connected with an opening of the culture structure through a first pipeline, an oxygen detector is arranged on the first pipeline, and the oxygen detector is electrically connected to the control system; A first valve and a second valve are provided on the first pipeline, the first pipeline on one side of the first valve is connected to one end of the second pipeline, the first pipeline on one side of the second valve is connected to one end of the third pipeline, the other end of the second pipeline and the other end of the third pipeline are both connected to the outside atmosphere, the second pipeline is provided with a third valve, the third pipeline is provided with a fourth valve, and the first valve, the second valve, the third valve and the fourth valve are all electrically connected to the control system; Alternatively, a first valve and a second valve are provided on the first pipeline, and both the first valve and the second valve are two-position three-way gas solenoid valves, and both the first valve and the second valve include a common port and two non-common ports, and the common ports of the first valve and the second valve are both connected to one end of the first pipeline, and one non-common port of the first valve and the second valve is both connected to the other end of the first pipeline, and the other non-common ports of the first valve and the second valve are both connected to the outside atmosphere; A detection probe group is provided in the culture structure, and the detection probe group is electrically connected to the control system. The detection probe group includes a pH probe, a DO probe and a chlorophyll monitoring probe. The pH probe is used to monitor the pH of the algae liquid, the DO probe is used to monitor the dissolved oxygen content of the algae liquid, and the chlorophyll monitoring probe is used to monitor the concentration of the algae liquid; The first pipeline is provided with a gas buffer tank, and both ends of the gas buffer tank are connected with both ends of the fourth pipeline. The oxygen detector is provided on the fourth pipeline, and a gas dryer is further provided on the fourth pipeline, and the gas dryer is provided upstream of the oxygen detector. The gas buffer tank is provided with an air pressure sensor, and the air pressure sensor is electrically connected to the control system.

2. The gas internal circulation algae photosynthetic oxygen production testing device according to claim 1, characterized in that: An illumination device is arranged outside the culture structure, the illumination device is electrically connected to the control system, and the illumination device is used to illuminate the algae in the culture structure.

3. The gas internal circulation algae photosynthetic oxygen production testing device according to claim 1, characterized in that: The gas internal circulation algae photosynthetic oxygen production testing device also includes a cooling structure, which includes a cooler, which is electrically connected to the control system. A cooling pipe is arranged in the culture structure, one end of the cooler is connected to one end of the cooling pipe through a fifth pipe, and the other end of the cooler is connected to the other end of the cooling pipe through a sixth pipe.

4. The gas internal circulation type algae photosynthetic oxygen production testing device according to claim 1, characterized in that: The gas internal circulation type algae photosynthetic oxygen production testing device also includes a feeding structure, and the feeding structure is connected to the culture structure through a seventh pipeline.

5. The gas internal circulation type algae photosynthetic oxygen production testing device according to claim 4, characterized in that: The seventh pipeline is provided with a feed pump, and the feed pump is electrically connected to the control system.

6. The gas internal circulation algae photosynthetic oxygen production testing device according to claim 1, characterized in that: The first pipeline is provided with a gas circulation pump, and the gas circulation pump is electrically connected to the control system.

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