A sophisticated device for rapid polymerase chain reaction

Abstract

The present invention discloses a precision device for rapid polymerase chain reaction, including a heating unit, a sample carrier and a test tube. The heating unit includes a plurality of temperature conducting modules and a plurality of temperature variable stickers corresponding to each other; the temperature of the temperature variable stickers is different; a plurality of slots are provided on the temperature conducting module, and the plurality of slots are connected at the center of the projection of the bottom end of the temperature conducting module to form a straight line; the test tube includes a plurality of sample tubes, and the plurality of sample tubes can be inserted into the plurality of slots provided on any temperature conducting module corresponding to each other. The present invention sets different temperature points required for PCR reaction on the temperature conducting module. During the reaction process, the sample tube is directly inserted into the slot according to different steps of the reaction, so that the DNA polymerase in the sample tube quickly reaches the required temperature. Since the temperature conducting module is always at the required temperature point, it is not necessary to heat again during the process of changing the temperature of the sample tube, thereby avoiding the occurrence of temperature surge phenomenon and ensuring the accuracy of the experimental results.

Description

Technical Field

[0001] The invention relates to the technical field of detection instruments, and in particular to a precision device for rapidly performing polymerase chain reaction. Background Art

[0002] PCR (polymerase chain reaction) was invented by Kary Mullis, who won the Nobel Prize in Chemistry seven years later in October 1993. The technique uses a human intervention method to amplify specific DNA fragments by repeating the same process repeatedly using a special enzyme, DNA (deoxyribo nucleic acid) polymerase. DNA polymerase exists naturally in organisms and replicates itself before cell division. When DNA begins to replicate, helicase separates the double-stranded DNA into two single strands. DNA polymerase binds to the two DNA strands to generate complementary strands. In Mullis's original PCR reaction, DNA polymerase was used in vitro. Double-stranded DNA was heated to 96°C, causing the double strands to separate into two single strands. However, at this temperature, DNA polymerase is destroyed, so new polymerase must be added after each heating step of the cycle. Mullis's original PCR reaction was extremely inefficient, required a lot of time and DNA polymerase, and required human supervision throughout the PCR reaction. Later, the DNA polymerase produced by the thermophilic bacterium Thermus aquaticus improved this inefficient PCR reaction. Since thermophilic bacteria live in geysers with temperatures reaching 50°C to 80°C (122 to 176°F), its DNA polymerase is heat-resistant. When used in PCR reactions, high temperatures do not destroy this DNA polymerase, so there is no need to constantly add new polymerase, making the PCR reaction simple and machine-operated. The birth of the PCR instrument solved the cumbersome amplification process, and the machine achieved effective amplification entirely through temperature changes.

[0003] PCR amplifier, also known as PCR gene amplifier, PCR nucleic acid amplifier, polymerase chain reaction nucleic acid amplifier, is an instrument that uses PCR technology to amplify specific DNA. PCR amplifier is usually composed of a thermal cover component, a thermal cycle component, a transmission component, a control component, and a power supply component.

[0004] Figure 1The figure is a schematic diagram of a PCR instrument on the market, which mainly includes a sample pool 10, a sample pool cover 20, a control panel 30, a display 40, an indicator light 50, a power switch 60 and a body 70. At present, all PCR instruments on the market use semiconductor heating and cooling to perform amplification reactions. When this method is used, the temperature change time is long during the heating and cooling process. Generally, one cycle reaction needs to go through 20-25 seconds during the heating and cooling process. Conventional PCR requires 40 cycles of reaction, and the total time takes 800s-1000s. The entire reaction time is long, which has a great impact on the PCR product. In order to improve the accuracy and rate of temperature change, it is necessary to select a high-cost semiconductor patch and a temperature conduction module with rapid temperature transfer (the most expensive one is a gold-plated module at present), which increases the equipment cost. In addition, since the temperature surge phenomenon occurs during the semiconductor heating and cooling process, the amplification conditions are changed, which has a great impact on the accurate amplification of PCR. Summary of the invention

[0005] The purpose of the present invention is to provide a precise device for rapidly performing polymerase chain reaction to solve the above-mentioned problem, prevent the temperature surge phenomenon during the PCR reaction process, and reduce the PCR reaction time to improve the experimental efficiency.

[0006] The present invention provides a precision device for rapid polymerase chain reaction, comprising a heating unit, a sample carrier and a test tube; the heating unit comprises a plurality of temperature conduction modules and a plurality of temperature variable stickers, the temperature conduction modules and the temperature variable stickers correspond one to one; the temperatures of the temperature variable stickers are different; the bottom end of the temperature conduction module is fixedly connected to the top end of the temperature variable sticker; the bottom end of the temperature variable sticker is fixedly connected to the top end of the sample carrier; a plurality of slots are provided on the temperature conduction module, the plurality of slots are connected at the center of the projection of the bottom end of the temperature conduction module to form a straight line; the test tube comprises a plurality of sample tubes, the plurality of sample tubes can be inserted one by one into the plurality of slots provided on any of the temperature conduction modules.

[0007] In one embodiment, the thermal conduction module includes a heat conducting part and a heat insulating part, wherein the heat insulating part is coated on the periphery of the heat conducting part; the bottom end of the heat insulating part is fixedly connected to the top end of the temperature variable sticker, and the heat conducting part is provided with a plurality of the slots.

[0008] In one embodiment, the test tube further comprises a connecting sheet, and the open ends of the plurality of sample tubes are fixedly clamped on the connecting sheet.

[0009] In one embodiment, the precision equipment for rapid polymerase chain reaction also includes a first rotating device and a lifting device; multiple sample tubes are arranged on the lifting device; the center of the bottom end of the sample carrier is fixedly connected to the first rotating device, and the first rotating device can drive the sample carrier to rotate; a circular accommodating area with the center as the center is provided on the sample carrier, and the circular accommodating area includes multiple fan-shaped areas; any adjacent temperature conduction modules are distributed at equal angles on one of the fan-shaped areas; the extension lines of the straight lines formed by the temperature conduction modules intersect at the center; after the lifting device drives multiple sample tubes to move upward to disengage from the slots, the first rotating device drives the sample carrier to rotate so that another adjacent temperature conduction module is located below the multiple sample tubes; the lifting device drives multiple sample tubes to move downward to be inserted into the multiple slots of another temperature conduction module.

[0010] In one embodiment, the precision equipment for rapid polymerase chain reaction includes a plurality of the heating units, and the lifting device includes a plurality of clamping tables; the plurality of the heating units are distributed at equal angles on a plurality of the fan-shaped areas; and the plurality of the clamping tables correspond one-to-one to the plurality of the heating units.

[0011] In one embodiment, the precision equipment for rapid polymerase chain reaction includes six heating units, the lifting device includes six clamping tables; the circular accommodating area includes six fan-shaped areas; the six heating units are distributed at equal angles on the six fan-shaped areas; the six clamping tables correspond one to one with the six heating units.

[0012] In one embodiment, the precision equipment for rapid polymerase chain reaction also includes a second rotating device and a lifting device; the lifting device is connected to the second rotating device; a plurality of the sample tubes are arranged on the lifting device; a circular accommodating area with the center as the center is provided on the sample carrier, and the circular accommodating area includes a plurality of fan-shaped areas; any adjacent temperature conduction modules are distributed at equal angles on one of the fan-shaped areas; the extension lines of the straight lines formed by the temperature conduction modules intersect at the center; after the lifting device drives the plurality of the sample tubes to move upward to disengage from the slots, the second rotating device drives the lifting device to rotate so that the plurality of the sample tubes are located above another adjacent temperature conduction module; the lifting device drives the plurality of the sample tubes to move downward to be inserted into the plurality of slots of another temperature conduction module.

[0013] In one embodiment, the precision equipment for rapid polymerase chain reaction also includes a moving device and a lifting device; a plurality of the sample tubes are arranged on the lifting device; the temperature conduction modules are evenly spaced on the sample carrier; the straight lines formed by the temperature conduction modules are parallel to each other; the bottom end of the sample carrier is connected to the moving device; after the lifting device drives the plurality of the sample tubes to move upward to disengage from the slots, the moving device drives the sample carrier to move so that the plurality of the sample tubes are located below another adjacent temperature conduction module; the lifting device drives the plurality of the sample tubes to move downward to be inserted into the plurality of slots of another temperature conduction module; or, the lifting device is connected to the moving device; after the lifting device drives the plurality of the sample tubes to move upward to disengage from the slots, the moving device drives the lifting device to move so that the plurality of the sample tubes are located above another adjacent temperature conduction module; the lifting device drives the plurality of the sample tubes to move downward to be inserted into the plurality of slots of another temperature conduction module.

[0014] In one embodiment, the lifting device includes a lifting block and a clamping platform, the clamping platform includes a cover plate and two clamping parts, the two clamping parts are respectively fixed on two opposite sides of the cover plate, and a clamping platform is formed between the clamping parts and the cover plate; one of the clamping parts is fixedly connected to the lifting block; when the clamping platform clamps the connecting sheet, the cover plate is pressed on the open end of the sample tube.

[0015] In one embodiment, the heating unit further includes a plurality of fan modules, and each of the fan modules is fixed one by one at the bottom end of each of the temperature-changing stickers.

[0016] In one embodiment, the temperature of the cover plate is between 95°C and 105°C.

[0017] In one embodiment, eight slots are formed on the thermal conduction module.

[0018] The precision equipment for rapid polymerase chain reaction provided by the present invention comprises a heating unit, a sample carrier and a test tube. The heating unit comprises three temperature conducting modules and three temperature variable stickers, and the three temperature conducting modules correspond to the three temperature variable stickers one by one; the temperatures of the three temperature variable stickers are different; the bottom end of the temperature conducting module is fixedly connected to the top end of the temperature variable sticker; the bottom ends of the three temperature variable stickers are fixedly connected to the top end of the sample carrier; the temperature conducting module is provided with a plurality of slots, and the plurality of slots are connected to form a straight line at the center of the projection of the bottom end of the temperature conducting module; the test tube comprises a plurality of sample tubes, and the plurality of sample tubes can be inserted one by one into the plurality of slots provided on any of the temperature conducting modules. In the present invention, three temperature points required for PCR reaction are set for three temperature conduction modules. During the reaction, according to different steps of the reaction, the sample tube is directly inserted into the slot hole, so that the DNA polymerase in the sample tube quickly reaches the required temperature. Since the temperature conduction module is always at the required temperature point, there is no need to heat the sample tube again during the process of changing the temperature, thereby avoiding the temperature surge phenomenon and ensuring the accuracy of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of an existing PCR instrument;

[0020] Figure 2 A top view of a temperature conducting module in a precision device for rapidly performing polymerase chain reaction provided by an embodiment of the present invention;

[0021] Figure 3 A side view of a temperature conducting module in a precision device for rapidly performing polymerase chain reaction provided by an embodiment of the present invention;

[0022] Figure 4 A first schematic diagram of the working state of the sample loading platform in the precision equipment for rapid polymerase chain reaction provided by an embodiment of the present invention;

[0023] Figure 5 A second schematic diagram of the working state of the sample loading platform in the precision equipment for rapid polymerase chain reaction provided by an embodiment of the present invention;

[0024] Figure 6 A schematic diagram of a lifting device in a precision device for rapidly performing polymerase chain reaction provided by an embodiment of the present invention;

[0025] Figure 7 A schematic diagram of another state of the lifting device in the precision equipment for rapid polymerase chain reaction provided by an embodiment of the present invention;

[0026] Figure 8 A schematic diagram of a clamping table in a precision device for rapidly performing polymerase chain reaction provided by an embodiment of the present invention;

[0027] Fig. 9 A schematic diagram of the operating interface of a precision device for rapidly performing polymerase chain reaction provided in an embodiment of the present invention.

[0028] Description of Reference Numerals

[0029] 10-sample pool; 20-sample pool cover; 30-control panel; 40-display; 50-indicator light; 60-power switch; 70-body; 100-heating unit; 110-temperature conduction module; 111-slot; 112-heat conduction part; 113-insulation part; 120-temperature variable sticker; 130-fan module; 200-sample loading platform; 210-center; 220-circular accommodating area; 221-fan-shaped area; 300-test tube; 310-sample tube; 320-connecting sheet; 400-first rotating device; 500-lifting device; 510-lifting block; 520-clamping table; 521-cover plate; 522-clamping part; 523-card table; 530-lifting motor; 540-screw slide rail; 600-operating interface. DETAILED DESCRIPTION

[0030] Embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be interpreted as limiting the present invention.

[0031] like Figures 2 to 8As shown, the precise device for rapidly performing polymerase chain reaction (also referred to as a rapid PCR instrument) provided by the embodiment of the present invention comprises a heating unit 100 , a sample carrier 200 and a test tube 300 . Among them, the heating unit 100 includes multiple temperature conducting modules 110 and multiple temperature variable stickers 120 (the figure shows three temperature conducting modules 110 and corresponding three temperature variable stickers 120 as an example. This embodiment takes three temperature conducting modules and three temperature variable stickers as an example, but it should be noted that the number of temperature conducting modules and temperature variable stickers can be set by oneself), the three temperature conducting modules 110 and the three temperature variable stickers 120 correspond one to one; the temperatures of the three temperature variable stickers 120 are different; the bottom end of the temperature conducting module 110 is fixedly connected to the top end of the temperature variable sticker 120; the bottom ends of the three temperature variable stickers 120 are fixedly connected to the top end of the sample carrier 200; a plurality of slots 111 are opened on the temperature conducting module 110, and the plurality of slots 111 are connected to form a straight line at the center 210 of the projection of the bottom end of the temperature conducting module 110; the test tube 300 includes a plurality of sample tubes 310, and the plurality of sample tubes 310 can be inserted one by one into the plurality of slots 111 opened on any temperature conducting module 110. In this embodiment, since the temperature of the temperature conducting module 110 is preset in advance, the material requirements for the temperature variable sticker 120 and the temperature conducting module 110 do not need to be too strict. Ordinary temperature variable stickers 120 and temperature conducting modules 110 can be used, which can greatly save costs.

[0032] It is known to those skilled in the art that during the PCR reaction, the volume of the sample tube 310 is very small. In this embodiment, the capacity of the sample tube 310 is 0.2 ml. Therefore, when the sample tube 310 is inserted into the slot 111 of the temperature conducting module 110, the temperature of the sample in the sample tube 310 can quickly reach the same temperature as the temperature conducting module 110, and will not affect the temperature set by the temperature conducting module 110. In this embodiment, the temperatures of the three temperature variable stickers 120 are set to 95°C, 55°C and 72°C respectively. This temperature is the temperature that the DNA polymerase needs to pass during the PCR reaction.

[0033] In this embodiment, the temperature of the temperature conducting module 110 is controlled by a temperature variable sticker 120 disposed at the bottom of the temperature conducting module 110, so that the temperature conducting module 110 is maintained at a set temperature. Those skilled in the art may also understand that in an experiment, multiple samples will be used for comparison. Therefore, each temperature conducting module 110 in this embodiment is provided with a plurality of slots 111, so that multiple samples can be amplified at one time, thereby speeding up the process of the experiment. In this embodiment, the number of slots 111 is preferably 8. Of course, those skilled in the art may also increase or decrease the number of slots 111 according to the needs of the experiment. In this embodiment, since there are temperature variable stickers 120 and temperature conducting modules 110 on the sample carrier 200, the material of the sample carrier 200 should be a high temperature resistant material.

[0034] In the embodiment of the present invention, three temperature points required for the PCR reaction are set at 95°C, 55°C and 72°C for the three temperature conducting modules 110. During the reaction, the DNA polymerase first stays in the temperature conducting module 110 at 95°C for 10 seconds, then stays in the temperature conducting module 110 at 55°C for 10 seconds, and finally stays in the temperature conducting module 110 at 72°C for 10 seconds, thus completing one cycle. Since more DNA polymerase is required each time, multiple cycles are required to complete the entire amplification process. For example, in the present embodiment, 30 cycles are required to complete one amplification reaction. It is only necessary to repeat the above cycles, which is convenient and simple to operate. During each temperature change, the DNA polymerase only needs to directly insert the sample tube 310 into the slot 111. The volume of the sample tube 310 is very small relative to the temperature conducting module 110. Therefore, the DNA polymerase in the sample tube 310 can quickly reach the required temperature without changing the temperature of the temperature conducting module 110. Since the temperature conducting module 110 is always at the set temperature point, there is no need to heat the sample tube 310 again during the temperature change, thereby avoiding the temperature surge phenomenon and ensuring the accuracy of the experimental results.

[0035] In one embodiment, the thermal conduction module 110 includes a thermal conduction portion 112 and a thermal insulation portion 113, wherein the thermal insulation portion 113 is coated on the outer periphery of the thermal conduction portion 112; the bottom end of the thermal insulation portion 113 is fixedly connected to the top end of the temperature-changing sticker 120, and the thermal conduction portion 112 is provided with a plurality of slots 111. The thermal insulation portion 113 can effectively maintain the temperature of the thermal conduction portion 112 and prevent the temperature of the thermal conduction portion 112 from decreasing, so as to ensure that the temperature of the slot 111 is at the set temperature each time the sample tube 310 is inserted into the slot 111. At the same time, since the spacing between the thermal conduction modules 110 is small during the experiment, and the temperatures of adjacent thermal conduction modules 110 are different, the thermal insulation portion 113 can also prevent the temperatures of adjacent thermal conduction modules 110 from affecting each other, thereby further ensuring the accuracy of the experimental results.

[0036] Those skilled in the art can understand that the material of the heat conducting part 112 should have good thermal conductivity to ensure that after the sample tube 310 is inserted into the slot 111, the sample in the sample tube 310 can quickly reach the same temperature as the heat conducting part 112. In this embodiment, the material of the heat conducting part 112 is copper, which has excellent thermal conductivity. In this embodiment, the heat insulating part 113 is preferably a rubber pad.

[0037] In one embodiment, the test tube 300 further includes a connecting sheet 320, and the open ends of the plurality of sample tubes 310 are fixedly clamped on the connecting sheet 320. The plurality of sample tubes 310 are connected by the connecting sheet 320, so that the plurality of sample tubes 310 can be conveniently taken at one time, simplifying the operation.

[0038] In one embodiment, the precision equipment for rapid polymerase chain reaction also includes a first rotating device 400 and a lifting device 500; a plurality of sample tubes 310 are arranged on the lifting device 500; the center 210 at the bottom end of the sample carrier 200 is fixedly connected to the first rotating device 400, and the first rotating device 400 can drive the sample carrier 200 to rotate; a circular accommodating area 220 with the center 210 as the center is provided on the sample carrier 200, and the circular accommodating area 220 can include a plurality of fan-shaped areas 221; any adjacent temperature conducting The modules 110 are distributed at equal angles on one of the fan-shaped areas 221; the extension lines of the three straight lines formed by the three temperature conducting modules 110 intersect at the center 210; after the lifting device 500 drives the multiple sample tubes 310 to move upward to disengage from the slots 111, the first rotating device 400 drives the sample stage 200 to rotate so that another adjacent temperature conducting module 110 is located below the multiple sample tubes 310; the lifting device 500 drives the multiple sample tubes 310 to move downward to be inserted into the multiple slots 111 of another temperature conducting module 110. In this embodiment, the sample stage 200 is set to be circular, and the first rotating device 400 is used to drive the sample stage 200 to rotate, so that the size of the PCR instrument can be reduced, which is convenient for carrying and convenient for cycle testing. The first rotating device 400 is preferably a rotating electrode.

[0039] In one embodiment, the lifting device 500 includes a lifting block 510 and a clamping platform 520. The clamping platform 520 includes a cover plate 521 and two clamping parts 522. The two clamping parts 522 are respectively fixed on opposite sides of the cover plate 521. A clamping platform 523 is formed between the clamping parts 522 and the cover plate 521. The clamping part 522 close to the center 210 is fixedly connected to the lifting block 510 on the side away from the cover plate 521. When the clamping platform 523 clamps the connecting sheet 320, the cover plate 521 covers the open end of the sample tube 310. During the experiment, since the sample tube 310 needs to be frequently taken out and put in the slot 111, it is inevitable that the sample will overflow from the opening of the sample tube 310. To avoid this phenomenon, the embodiment of the present invention is provided with a cover plate 521. During the process of taking the sample tube 310, the cover plate 521 covers the opening of the sample tube 310 to avoid sample overflow, thereby improving the accuracy of the experiment.

[0040] In this embodiment, the lifting device 500 also includes a lifting motor 530 and a screw rail 540. The lifting block 510 is threadedly connected to the screw rail 540. The lifting motor 530 drives the screw rail 540 to rotate so that the lifting block 510 slides on the screw rail 540, thereby achieving the purpose of taking the sample tube 310 out of or putting it into the slot 111. The lifting distance and the lifting speed are adjusted by the screw rail 540. In this embodiment, the distance adjustment of the screw rail 540 can be accurate to 0.1 mm.

[0041] In one embodiment, the temperature of the cover plate 521 is between 95° C. and 105° C. The cover plate 521 is pressed against the opening of the sample tube 310. During the sample heating process, the steam in the sample will inevitably gather on the cover plate 521, thereby affecting the experimental results. By setting the temperature of the cover plate 521 between 95° C. and 105° C., the water vapor condensed on the cover plate 521 can be evaporated, thereby further ensuring the accuracy of the experimental results. In this embodiment, the temperature of the cover plate 521 is preferably 100° C.

[0042] The circular receiving area 220 can be equally divided into a plurality of sector-shaped areas 221, so that the circular area can accommodate a plurality of heating units 100 at one time, which will be described in detail below:

[0043] The precision equipment for rapid polymerase chain reaction includes a plurality of heating units 100, and the lifting device 500 includes a plurality of one-to-one corresponding clamping tables 520 and lifting blocks 510; the plurality of heating units 100 are distributed at equal angles on the plurality of fan-shaped areas 221; the plurality of clamping tables 520 correspond to the plurality of heating units 100 one-to-one, and the plurality of clamping tables 520 are fixedly connected to the lifting blocks 510 at equal angles. In this embodiment, by providing a plurality of heating units 100, amplification reactions of multiple groups of DNA polymerases can be performed at one time to save experimental time.

[0044] In one embodiment, the precision equipment for rapid polymerase chain reaction includes six heating units 100, and the lifting device 500 includes six clamping tables 520; the circular accommodating area 220 can be equally divided into six fan-shaped areas 221, and the two sides of the fan-shaped area 221 meet at the center 210 of the circular accommodating area 220, and the angle between the two sides of each fan-shaped area 221 is 60 degrees. The six heating units 100 are distributed at equal angles on the six fan-shaped areas 221; and the straight line formed by any temperature conducting module 110 in each heating unit 100 passes through the center 210; the six clamping tables 520 correspond to the six heating units 100 one by one, and the six clamping tables 520 are fixedly connected to the lifting block 510 at equal angles.

[0045] For example, when eight slots 111 are provided on the heating module, one test tube 300 includes eight sample tubes 310. At this time, the present embodiment can provide 48 sample amplifications at the same time, and can perform amplification reactions on 48 samples at the same time. Figure 4, divided into six groups A, B, C, D, E, and F in a counterclockwise direction, each group can independently set different temperatures to achieve gradient amplification reactions, or set the same temperature to achieve isocratic amplification reactions, each group of temperature conducting modules 110 is provided with 8 slots 111, each group is numbered in a counterclockwise direction, and the three temperature conducting modules 110 are numbered A1-A2-A3, B1-B2-B3, C1-C2-C3, D1-D2-D3, E1-E2-E3, and F1-F2-F3. The following will take 48 sample amplification as an example for explanation:

[0046] First, set the A1, B1, C1, D1, E1, and F1 temperature conduction modules 110 to a constant 95° C., set the A2, B2, C2, D2, E2, and F2 temperature conduction modules 110 to a constant 55° C., and set the A3, B3, C3, D3, E3, and F3 temperature conduction modules 110 to a constant 72° C.

[0047] Next, the lifting block 510 moves downward to deliver the eight rows of sample tubes 310 into the slots 111 of the temperature conducting modules 110 numbered A1, B1, C1, D1, E1, and F1, and stops for 10 seconds; then, the lifting block 510 moves upward until the eight rows of sample tubes 310 leave the slots 111, then, the rotary motor rotates 20° clockwise, at which time the eight rows of sample tubes 310 are facing the slots 111 of the temperature conducting modules 110 numbered A2, B2, C2, D2, E2, and F2, then, the lifting block 510 descends. Until the sample tube 310 enters the slot 111 and remains stationary for 10 seconds, then the lifting block 510 moves upward until the eight rows of sample tubes 310 leave the slot 111, then the rotating motor rotates 20° clockwise, at this time, the slots 111 of the temperature conduction module 110 numbered A3, B3, C3, D3, E3, and F3 are under the eight rows of sample tubes 310, the lifting block 510 descends into the slot 111 and remains stationary for 10 seconds, then the lifting block 510 rises to drive the sample tube 310 to leave the slot 111, thus completing a cycle reaction.

[0048] For isothermal amplification reaction, the lifting block 510 and the rotating disk move interactively in the above manner, rotating 360° to complete 6 cycles. Generally, an amplification reaction requires 30 cycles, and amplification can be completed by completing only 5 360° rotations.

[0049] For gradient temperature amplification reactions, the temperature of each group A, B, C, D, E, and F is different. After completing a cycle, the rotary motor only needs to rotate 40° counterclockwise to return to the initial position. Repeating the cycle 30 times can complete 30 rounds of PCR amplification reactions.

[0050] The PCR instrument provided in this embodiment uses the lifting block 510 and the rotating motor to assist in placing or removing the sample tube 310 from the slot 111, which greatly saves the test time. Since the temperature of the temperature conducting module 110 is pre-set, the sample will not experience a temperature surge during the temperature change process, thereby ensuring the accuracy of the sample results.

[0051] In another embodiment, the precision equipment for rapid polymerase chain reaction further comprises a second rotating device and a lifting device 500; a plurality of sample tubes 310 are arranged on the lifting device 500; a circular accommodating area 220 with the center 210 as the center is provided on the sample carrier 200; the circular accommodating area 220 may include a plurality of fan-shaped areas 221, and any adjacent temperature conducting modules 110 are distributed at equal angles on any fan-shaped area 221; the extension lines of the three straight lines formed by the three temperature conducting modules 110 intersect at the center 210; after the lifting device 500 drives the plurality of sample tubes 310 to move upward to disengage from the slot 111, the second rotating device drives the lifting device 500 to rotate so that the plurality of sample tubes 310 are located above another adjacent temperature conducting module 110; the lifting device 500 drives the plurality of sample tubes 310 to move downward to be inserted into the plurality of slots 111 of another temperature conducting module 110. The difference between this embodiment and the previous embodiment is that the sample carrier 200 is fixed, and the lifting device 500 rotates under the drive of the second rotating device. Of course, the user can make settings according to the actual situation as to how to choose.

[0052] In another embodiment, the precision equipment for rapid polymerase chain reaction further includes a moving device and a lifting device 500; a plurality of sample tubes 310 are arranged on the lifting device 500; three temperature conducting modules 110 are evenly spaced and distributed on the sample stage 200; three straight lines formed by the three temperature conducting modules 110 are parallel to each other; the bottom end of the sample stage 200 is connected to the moving device; after the lifting device 500 drives the plurality of sample tubes 310 to move upward to disengage from the slots 111, the moving device drives the sample stage 200 to move so that the plurality of sample tubes 310 are located below another adjacent temperature conducting module 110; the lifting device 500 drives the plurality of sample tubes 310 to move downward to be inserted into the plurality of slots 111 of another temperature conducting module 110. For some narrow and long spaces, this embodiment provides a solution in which the temperature conducting modules 110 are arranged in parallel.

[0053] In another embodiment, the precision equipment for rapid polymerase chain reaction also includes a moving device and a lifting device 500; a plurality of sample tubes 310 are arranged on the lifting device 500; three temperature conducting modules 110 are evenly spaced and distributed on the sample carrier 200; three straight lines formed by the three temperature conducting modules 110 are parallel to each other; the lifting device 500 is connected to the moving device; after the lifting device 500 drives the plurality of sample tubes 310 to move upward to disengage from the slots 111, the moving device drives the lifting device 500 to move so that the plurality of sample tubes 310 are located above another adjacent temperature conducting module 110; the lifting device 500 drives the plurality of sample tubes 310 to move downward to be inserted into the plurality of slots 111 of another temperature conducting module 110. The difference between this embodiment and the previous embodiment is that in this embodiment, the sample carrier 200 is stationary, and the lifting device 500 can move while lifting and lowering.

[0054] In one embodiment, the heating unit 100 further includes three fan modules 130, which are fixed one by one to the bottom ends of the three temperature-changing stickers 120. The fan modules 130 can be used to adjust the temperature of the temperature-conducting module 110 so that the temperature-conducting module 110 is always at a set temperature.

[0055] In one embodiment, the precision equipment for rapid polymerase chain reaction further includes a smart chip, which is used to control the operation of the temperature conducting module 110, the temperature variable sticker 120, the fan module 130, the lifting device 500, the first rotating device 400, and the second rotating device. Fig. 9 As shown, in this embodiment, an operation interface 600 is also provided, and the experimenter can input the temperature, reaction time and reaction type to be set in the operation interface 600 to complete the PCR reaction, thereby further increasing the intelligence of the PCR instrument.

[0056] The above describes in detail the structure, features and effects of the present invention based on the embodiments shown in the drawings. The above are only preferred embodiments of the present invention, but the present invention is not limited to the scope of implementation shown in the drawings. Any changes made according to the concept of the present invention, or modifications to equivalent embodiments with equivalent changes, which still do not exceed the spirit covered by the description and drawings, should be within the protection scope of the present invention.

Claims

1. A precision device for rapid polymerase chain reaction, characterized in that: It includes a heating unit, a sample carrier and a test tube; The heating unit includes a plurality of temperature conducting modules and a plurality of temperature variable stickers, the temperature conducting modules and the temperature variable stickers correspond to each other one by one, and the temperature of the temperature conducting modules is preset in advance; the temperature of each temperature variable sticker is different; the bottom end of the temperature conducting module is fixedly connected to the top end of the temperature variable sticker; the bottom end of the temperature variable sticker is fixedly connected to the top end of the sample carrier; The temperature conducting module is provided with a plurality of slots, and the plurality of slots are connected at the center of the projection of the bottom end of the temperature conducting module to form a straight line; the test tube includes a plurality of sample tubes, and the plurality of sample tubes can be inserted into any of the plurality of slots provided on the temperature conducting module in a one-to-one correspondence; The test tube also includes a connecting sheet, and the open ends of the plurality of sample tubes are fixedly clamped on the connecting sheet; The precision equipment for rapid polymerase chain reaction also includes a first rotating device and a lifting device; a plurality of the sample tubes are arranged on the lifting device; The center of the bottom end of the sample carrier is fixedly connected to the first rotating device, and the first rotating device can drive the sample carrier to rotate; a circular accommodating area with the center as the center is provided on the sample carrier, and the circular accommodating area includes a plurality of fan-shaped areas; any adjacent temperature conduction modules are distributed at equal angles on one of the fan-shaped areas; the extension lines of the straight lines formed by the temperature conduction modules intersect at the center; After the lifting device drives the plurality of sample tubes to move upward to disengage from the slots, the first rotating device drives the sample carrier to rotate so that another adjacent temperature conducting module is located below the plurality of sample tubes; the lifting device drives the plurality of sample tubes to move downward to be inserted into the plurality of slots of another temperature conducting module; The lifting device includes a lifting block and a clamping platform. The clamping platform includes a cover plate and two clamping parts. The two clamping parts are respectively fixed on two opposite sides of the cover plate, and a clamping platform is formed between the clamping parts and the cover plate. The clamping part close to the center is fixedly connected to the lifting block on the side away from the cover plate. When the clamping platform clamps the connecting sheet, the cover plate is pressed on the open end of the sample tube to prevent the sample from overflowing.

2. The precision equipment for rapid polymerase chain reaction according to claim 1, characterized in that: The thermal conduction module comprises a thermal conduction portion and a thermal insulation portion, wherein the thermal insulation portion is coated on the periphery of the thermal conduction portion; The bottom end of the heat insulating part is fixedly connected to the top end of the temperature variable sticker, and the heat conducting part is provided with a plurality of the slots.

3. The precision equipment for rapid polymerase chain reaction according to claim 1, characterized in that: The test tube also includes a connecting sheet, and the open ends of the plurality of sample tubes are fixedly clamped on the connecting sheet.

4. The precision equipment for rapid polymerase chain reaction according to claim 1, characterized in that: The precision equipment for rapid polymerase chain reaction comprises a plurality of the heating units, and the lifting device comprises a plurality of clamping tables; The plurality of heating units are distributed at equal angles on the plurality of fan-shaped areas; and the plurality of clamping tables correspond one to one with the plurality of heating units.

5. The precision equipment for rapid polymerase chain reaction according to claim 4, characterized in that: The precision equipment for rapid polymerase chain reaction comprises six heating units, the lifting device comprises six clamping tables; the circular receiving area comprises six fan-shaped areas; The six heating units are distributed at equal angles on the six sector-shaped areas; the six clamping tables correspond one to one with the six heating units.

6. The precision equipment for rapid polymerase chain reaction according to claim 1, characterized in that: The precision equipment for rapid polymerase chain reaction further comprises a second rotating device and a lifting device; the lifting device is connected to the second rotating device; a plurality of the sample tubes are arranged on the lifting device; The sample carrier is provided with a circular accommodating area with the center as the center, and the circular accommodating area includes a plurality of sector-shaped areas; any adjacent temperature conduction modules are distributed at equal angles on one of the sector-shaped areas; and the extension lines of the straight lines formed by the temperature conduction modules intersect at the center; After the lifting device drives the multiple sample tubes to move upward to disengage from the slots, the second rotating device drives the lifting device to rotate so that the multiple sample tubes are located above another adjacent temperature conduction module; the lifting device drives the multiple sample tubes to move downward to be inserted into the multiple slots of another adjacent temperature conduction module.

7. The precision equipment for rapid polymerase chain reaction according to claim 1, characterized in that: The precision equipment for rapid polymerase chain reaction also includes a moving device and a lifting device; a plurality of the sample tubes are arranged on the lifting device; the temperature conducting modules are evenly spaced and distributed on the sample loading platform; the straight lines formed by the temperature conducting modules are parallel to each other; The bottom end of the sample carrier is connected to the moving device; after the lifting device drives the plurality of sample tubes to move upward to disengage from the slots, the moving device drives the sample carrier to move so that the plurality of sample tubes are located below another adjacent temperature conducting module; the lifting device drives the plurality of sample tubes to move downward to be inserted into the plurality of slots of another temperature conducting module; or, The lifting device is connected to the moving device; after the lifting device drives the multiple sample tubes to move upward to disengage from the slots, the moving device drives the lifting device to move so that the multiple sample tubes are located above another adjacent temperature conduction module; the lifting device drives the multiple sample tubes to move downward to be inserted into the multiple slots of another temperature conduction module.

8. The precise device for rapid polymerase chain reaction according to claim 1, characterized in that: The heating unit also includes a plurality of fan modules, and each of the fan modules is fixed one by one at the bottom end of each of the temperature-changing stickers.

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