A large-volume double-swing plastic bag cell bioreactor

Abstract

The present invention relates to the technical field of cell bioreactors, and particularly to a large-volume double-swing plastic bag cell bioreactor. The present invention includes: a swing box assembly, a support frame assembly, an elastic swing rod assembly, a swing basket assembly, a multi-functional edge-wrapped heat preservation tank, and a cell culture bag; the swing box assembly is installed on the support frame assembly; the swing box assembly is connected to the upper part of the swing basket assembly through the elastic swing rod assembly; both ends of the load-bearing shaft of the support frame assembly are connected with two swing straight handles, and the two swing straight handles are connected through a swing shaft at the bottom of the swing basket assembly; the multi-functional edge-wrapped heat preservation tank is arranged in the swing basket assembly, and the cell culture bag is arranged in the multi-functional edge-wrapped heat preservation tank.

Description

Technical Field

[0001] The present invention relates to the technical field of cell bioreactors, and particularly to a large-volume double-swing plastic bag cell bioreactor. Background Art

[0002] At present, the global biopharmaceutical industry has developed rapidly. Moreover, the outbreak and spread of the COVID-19 pandemic in 2020 caused a large number of people to be infected with the novel coronavirus disease, and a considerable number of patients died. This major epidemic has promoted the rapid development of the biopharmaceutical industry in the opposite direction. For the research and development and capacity expansion of COVID-19 vaccines, diagnostic reagents, antibody drugs, and cell therapy fields, a large amount of capital, manpower, and technology have been invested in the industrialization process of biopharmaceutical research and development and capacity expansion globally. Therefore, disposable cell bioreactors for large-scale and high-density cultivation of animal cells, which are the most important bioprocess equipment in the biopharmaceutical industry, have become the key link and bottleneck problem for capacity expansion. Disposable cell bioreactors largely determine the production capacity, product variety, and product cost of biopharmaceutical enterprises. When the working volume of existing swing-type cell bioreactors is relatively large, for example, above 50 liters, an impact force on the two end points of the left and right swings will be generated, hitting the ground, generating vibration and noise. When the volume further increases, for example, above 500 liters, the situation will be even more serious, and the workshop floor will vibrate accordingly, making the entire environment unable to work. Moreover, at a relatively large volume, it is not possible to reduce the swing frequency of the bioreactor in order to reduce the impact force and vibration, because doing so will reduce the flow rate of the culture medium in the culture bag, reduce the dissolved oxygen level, reduce the fluidity, reduce the mixing level, and increase harmful gases without overflowing. When accumulated too much, it will seriously affect the growth and amplification of cells. Therefore, swing-type cell bioreactors with a volume above 50 liters have been discontinued. Due to the above reasons, there is a need for existing swing-type cell bioreactors to eliminate defects such as impact force, vibration, and noise in order to carry out large-volume and large-scale cell culture.

[0003] The information disclosed in this background art section is only intended to deepen the understanding of the overall background art of the present invention, and should not be regarded as an admission or any form of suggestion that this information constitutes prior art known to those skilled in the art. Summary of the Invention

[0004] The purpose of the present invention is to provide a large-volume double-swing plastic bag cell bioreactor to solve the technical problems existing in existing swing-type cell bioreactors.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions:

[0006] The present invention provides a large-volume double-swing plastic bag cell bioreactor, which includes: a swing box assembly, a support frame assembly, an elastic swing rod assembly, a swing basket assembly, a multi-functional edge-wrapped heat preservation tank, and a cell culture bag;

[0007] The swing box assembly is installed on the support frame assembly; the swing box assembly is connected to the upper part of the swing basket assembly through the elastic swing rod assembly; both ends of the load-bearing shaft of the support frame assembly are connected with two swing straight handles, and the two swing straight handles are connected through the swing shaft at the bottom of the swing basket assembly; the multi-functional edge-wrapped heat preservation tank is arranged in the swing basket assembly, and the cell culture bag is arranged in the multi-functional edge-wrapped heat preservation tank.

[0008] Preferably, the swing box assembly includes: a swing box cover, a swing box body, a short swing rod shaft, a thrust joint bearing combined spherical mechanism, a linear guide rail, a ball screw, a stepping motor, a box body bottom plate, and a flange;

[0009] The swing box cover is connected to the swing box body through the flange; the stepping motor is arranged on the box body bottom plate of the swing box body; the stepping motor is connected to the screw of the ball screw, the nut of the ball screw is connected to the thrust joint bearing combined spherical mechanism, the thrust joint bearing combined spherical mechanism is slidably installed on the linear guide rail, the linear guide rail is installed on the box body bottom plate, and the short swing rod shaft is inserted through the thrust joint bearing combined spherical mechanism; the bottom plate of the swing box body is provided with a hollow part along the direction of the linear guide rail.

[0010] Preferably, the thrust joint bearing combined spherical mechanism includes: a housing, a shaft sleeve, and a thrust joint bearing; thrust joint bearings are respectively arranged at the upper and lower ends of the housing; the shaft sleeve is located between the upper and lower thrust joint bearings; the short swing rod shaft is sequentially inserted through the thrust joint bearing at the upper end, the shaft sleeve, and the thrust joint bearing at the lower end.

[0011] Preferably, the elastic swing rod assembly includes: a swing rod, an upper disc, four tension springs, a swing rod cross shaft, a swing rod cross shaft bearing, a tension spring fixing ring, and a lower disc;

[0012] The upper part of the swing rod passes through the central hole of the upper disc and is connected to the short swing rod shaft of the swing box assembly; the lower part of the swing rod is connected to the swing rod cross shaft; the swing rod cross shaft is installed on the lower disc through the swing rod cross shaft bearing; the upper ends of the four tension springs are respectively connected to the four corners of the upper disc; the lower ends of the four tension springs are respectively connected to the periphery of the lower disc through the tension spring fixing rings; the lower disc is connected to the swing frame of the swing basket assembly.

[0013] Preferably, the support frame assembly includes: a top frame beam, two end brackets, a swing straight handle bearing, a swing straight handle, a load-bearing shaft, a load-bearing shaft bearing, two end inclined support plates, a swing bearing, and a base bottom plate;

[0014] The top frame beam is connected to the base bottom plate through the two end brackets; the two end inclined support plates are respectively arranged on the sides of the two end brackets; both ends of the load-bearing shaft are connected to the two end brackets through the load-bearing shaft bearings; the upper ends of the two swing straight handles are both connected to the load-bearing shaft through the swing straight handle bearings; the lower ends of the two swing straight handles are both connected to the swing shaft through the swing bearings.

[0015] Preferably, rubber pads are arranged at the bottom of the base bottom plate.

[0016] Preferably, the swing basket assembly includes a swing frame and a swing shaft;

[0017] The upper part of the swing frame is connected to the lower part of the elastic swing rod assembly in a disk shape; a swing shaft is arranged at the bottom of the swing frame.

[0018] Preferably, the multi-functional edge-wrapped insulation tank is connected to the swing frame of the swing basket assembly through two end fixing platforms; the inner surface of the multi-functional edge-wrapped insulation tank is set as an arc surface adapted to the cell culture bag; the upper part of the multi-functional edge-wrapped insulation tank has an inwardly shrinking edge-wrapped structure; the multi-functional edge-wrapped insulation tank is fixed in the swing basket.

[0019] Preferably, the multi-functional edge-wrapped insulation tank is heated by a rubber plate or a water jacket.

[0020] Preferably, the cross-section of the cell culture bag is set as an ellipse; the plastic film seam of the cell culture bag is arranged at the position of its upper and lower diagonals.

[0021] Preferably, a plurality of liquid inlets, liquid outlets, air inlets, air outlets, and interfaces for installing dissolved oxygen, pH value, and temperature sensors are arranged at the upper part of the cell culture bag. The cell culture bag is made by welding a five-layer or seven-layer co-extruded plastic film.

[0022] Adopting the above technical solutions, the present invention has the following beneficial effects:

[0023] The large-volume double-swing plastic bag cell bioreactor provided by the present invention. First of all, the total weight of components such as the swinging basket during swinging and the culture medium acts on the load-bearing shaft through the swinging shaft and the swinging straight handle, and then the load acts on the ground through the support frame assembly. Secondly, for the movement of this double-swing mechanism, one is that the elastic swing rod swings left and right around the swinging shaft to drive the swinging basket assembly. The second is that the swinging shaft and the swinging straight handle swing left and right around the load-bearing shaft. Its advantage lies in that starting from the left and right swinging of the elastic swing rod around the swinging shaft, through the left and right swinging of the swinging basket, the culture medium in the cell culture bag also reciprocates in both left and right directions at the same time. Then, it comes to the left and right movement of the center of gravity of the swinging basket and the reverse swinging of the swinging shaft and the swinging straight handle around the load-bearing shaft. Finally, the load-bearing shaft transmits the load to the ground through the support frame assembly. During the entire movement process, according to the law of conservation of energy, only at several places with friction, there is a part where kinetic energy is converted into heat energy and consumed. These several rotating parts are all the rotations of the shaft and the bearing, with good lubrication, extremely small friction coefficient, and extremely little energy consumption. The rest of the energy is changed from kinetic energy into potential energy, then into swinging kinetic energy, and finally into normal pressure, acting on the ground. Basically, there is no impact force, shaking, or vibration generated. This completely solves the problem that when the existing mainstream swinging cell reactors in the world have a relatively large volume (above 50 liters), large impact forces, vibrations, shaking, and noises are generated when swinging to the left and right end points, especially when the volume is relatively large (above 500 liters), the situation is more serious. However, this large-volume double-swing plastic bag cell reactor of the present invention can stably culture cells under the conditions of no impact force, slight shaking and vibration, and slight noise. The working volume can reach 10,000 liters, and there will also be no large impact force and vibration. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0025] Figure 1 It is the front view of the large-volume double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0026] Figure 2 It is the side view of the large-volume double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0027] Figure 3 It is the top view of the large-volume double-swing plastic bag cell bioreactor provided by the embodiment of the present invention;

[0028] Figure 4 It is the front view of the swinging basket assembly provided by the embodiment of the present invention;

[0029] Figure 5 Side view of the swing basket assembly provided by the embodiment of the present invention;

[0030] Figure 6 Top view of the swing basket assembly provided by the embodiment of the present invention;

[0031] Figure 7 Front view of the swing box assembly provided by the embodiment of the present invention;

[0032] Figure 8 Top view of the swing box assembly provided by the embodiment of the present invention;

[0033] Figure 9 Front view of the elastic swing rod assembly provided by the embodiment of the present invention;

[0034] Figure 10 Top view of the elastic swing rod assembly provided by the embodiment of the present invention;

[0035] Figure 11 Front view of the multifunctional edge-wrapped heat preservation tank provided by the embodiment of the present invention;

[0036] Figure 12 Top view of the multifunctional edge-wrapped heat preservation tank provided by the embodiment of the present invention;

[0037] Figure 13 Structural schematic diagram of the thrust joint bearing combined spherical mechanism provided by the embodiment of the present invention;

[0038] Figure 14 Front view of the cell culture bag provided by the embodiment of the present invention;

[0039] Figure 15 Top view of the cell culture bag provided by the embodiment of the present invention;

[0040] Figure 16 Front view of the support frame assembly provided by the embodiment of the present invention;

[0041] Figure 17 Side view of the support frame assembly provided by the embodiment of the present invention;

[0042] Figure 18 Top view of the support frame assembly provided by the embodiment of the present invention;

[0043] Figure 19 Schematic diagram of the state when the large-volume double-swing plastic bag cell bioreactor provided by the embodiment of the present invention swings to the leftmost end;

[0044] Figure 20 Schematic diagram of the state when the large-volume double-swing plastic bag cell bioreactor provided by the embodiment of the present invention swings to the rightmost end. Detailed implementation manners

[0045] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0046] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0047] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0048] The following will describe in detail the specific implementation manners of the present invention with reference to the accompanying drawings. It should be understood that the specific implementation manners described herein are only used to illustrate and explain the present invention and are not used to limit the present invention.

[0049] Combined with Figures 1 to 20 As shown, this embodiment provides a large-volume double-swing plastic bag cell bioreactor, which includes: a swing box assembly 100, a support frame assembly 200, an elastic swing rod assembly 300, a swing basket assembly 400, a multi-functional edge-wrapped heat preservation tank 500, and a cell culture bag 600; the swing box assembly 100 is installed on the support frame assembly 200; the swing box assembly 100 is connected to the upper part of the swing basket assembly 400 through the elastic swing rod assembly 300; both ends of the load-bearing shaft 800 of the support frame assembly 200 are connected with two swing straight handles 210, and the two swing straight handles 210 are connected through the swing shaft 900 at the bottom of the swing basket assembly 400; the multi-functional edge-wrapped heat preservation tank 500 is arranged in the swing basket assembly 400, and the cell culture bag 600 is arranged in the multi-functional edge-wrapped heat preservation tank 500.

[0050] In this embodiment, preferably, the swing box assembly 100 includes: a swing box cover 101, a swing box body 102, a short swing rod shaft 112, a spherical mechanism 110 of a thrust joint bearing combination, a linear guide rail 104, a ball screw 106, a stepping motor 107, a box body bottom plate 108, and a flange 109; the swing box cover 101 is connected to the swing box body 102 through the flange 109; the stepping motor 107 is arranged on the box body bottom plate 108 of the swing box body 102; the stepping motor 107 is connected to the screw rod of the ball screw 106, the nut of the ball screw 106 is connected to the spherical mechanism 110 of the thrust joint bearing combination, the spherical mechanism 110 of the thrust joint bearing combination is slidably mounted on the linear guide rail 104, the linear guide rail 104 is mounted on the box body bottom plate 108, and the short swing rod shaft 112 is inserted through the spherical mechanism 110 of the thrust joint bearing combination; a hollow part is arranged on the bottom plate of the swing box body 102 along the direction of the linear guide rail 104. In this embodiment, the swing box adopts a spherical mechanism composed of a short swing rod shaft and a thrust joint bearing combination and its outer frame to cooperate to achieve linear reciprocating motion, linear movement up and down in the direction of the short shaft axis, and three-dimensional rotation.

[0051] In this embodiment, preferably, the spherical mechanism 110 of the thrust joint bearing combination is slidably mounted on two linear guide rails 104 through a sliding seat. Since the short swing rod shaft is the end of the rocker and has a long force arm and small force, the swing box has small force when swinging, so the swing box has a wide range of applications; of course, the swing box uses a ball screw mechanism to push and pull the swing rod reciprocally, and a hydraulic or pneumatic mechanism can also be used to replace this function. When selecting, it is mainly determined by factors such as reliability, noise level, and cost. Domestic bearings can be used for the spherical mechanism of the thrust joint bearing combination because its rotation speed is very low and the accuracy requirement is not high; it is better to use imported parts if conditions permit, and the transmission parts and linear guide rails are the main procurement parts.

[0052] In this embodiment, preferably, the spherical mechanism 110 of the thrust joint bearing combination includes: a housing 118, a bushing 116, and a thrust joint bearing 114; thrust joint bearings 114 are respectively arranged at the upper and lower ends of the housing 118; the bushing 116 is located between the upper and lower thrust joint bearings 114; the short swing rod shaft 112 is sequentially inserted through the upper thrust joint bearing 114, the bushing 116, and the lower thrust joint bearing 114.

[0053] In this embodiment, preferably, the elastic pendulum rod assembly 300 includes: a pendulum rod 302, an upper disc 304, four tension springs 306, a pendulum rod cross shaft 308, a pendulum rod cross shaft bearing 309, a tension spring fixing ring 320, and a lower disc 310; the upper part of the pendulum rod 302 passes through the central hole of the upper disc 304 and is connected to the pendulum rod short shaft 112 of the swing box assembly 100; the lower part of the pendulum rod 302 is connected to the pendulum rod cross shaft 308; the pendulum rod cross shaft 308 is installed on the lower disc 310 through the pendulum rod cross shaft bearing 309; the upper ends of the four tension springs 306 are respectively connected to the four corners of the upper disc 304; the lower ends of the four tension springs 306 are respectively connected to the periphery of the lower disc 310 through the tension spring fixing rings 320; the lower disc 310 is connected to the swing frame 402 of the swing basket assembly 400.

[0054] In this embodiment, preferably, the support frame assembly 200 includes: a top frame beam 202, two end brackets 204, a swing straight handle bearing 206, a swing straight handle 210, a load-bearing shaft 800, a load-bearing shaft bearing 806, two end inclined support plates 222, a swing bearing 902, and a base bottom plate 208; the top frame beam 202 is connected to the base bottom plate 208 through the two end brackets 204; the two end inclined support plates 222 are respectively arranged on the sides of the two end brackets 204; the two ends of the load-bearing shaft 800 are connected to the two end brackets 204 through the load-bearing shaft bearings 806; the upper ends of the two swing straight handles 210 are both connected to the load-bearing shaft 800 through the swing straight handle bearings 206; the lower ends of the two swing straight handles 210 are both connected to the swing shaft 900 through the swing bearings 902.

[0055] In this embodiment, preferably, a rubber pad is provided at the bottom of the base bottom plate 208.

[0056] In this embodiment, preferably, the swing basket assembly 400 includes a swing frame 402 and a swing shaft 900; the upper part of the swing frame 402 is connected to the lower disc 310 of the elastic pendulum rod assembly 300; a swing shaft 900 is provided at the bottom of the swing frame 402.

[0057] In this embodiment, preferably, the inner surface of the multi-functional edge-wrapped heat preservation tank 500 is set to an arc surface adapted to the cell culture bag; the upper part of the multi-functional edge-wrapped heat preservation tank 500 has an inwardly shrinking edge-wrapped structure; the multi-functional edge-wrapped heat preservation tank 500 is fixed in the swing frame 402 of the swing basket assembly 400. The multi-functional edge-wrapped heat preservation tank 500 is connected to the swing frame 402 of the swing basket assembly 400 through two end fixing platforms 508; the upper part of the multi-functional edge-wrapped heat preservation tank 500 has an inwardly shrinking edge-wrapped structure.

[0058] In this embodiment, preferably, the multi-functional edge-wrapped heat preservation tank 500 is heated by a rubber plate or a water jacket.

[0059] In this embodiment, preferably, the cross-section of the welded seals 606 at both ends of the cell culture bag 600 is set to an ellipse; the plastic film seams 602 of the cell culture bag 600 are set at the upper and lower diagonal positions. The upper part of the cell culture bag 600 is provided with a plurality of liquid inlets, liquid outlets, air inlets, air outlets and interfaces for installing dissolved oxygen, pH value and temperature sensors. The cell culture bag 600 is made by welding five or seven layers of co-extruded plastic film.

[0060] The motion analysis principle of the large-volume double-swing plastic bag cell bioreactor in this example:

[0061] 1. Movement principle of the reactor: The power source is the stepper motor and ball screw in the swing box, which push the spherical mechanism of the thrust joint shaft combination installed on the slide table to make reciprocating linear motion on the linear guide rail. The short shaft in the center of the spherical mechanism drives the elastic swing rod to swing left and right around the swing axis, and drives the swing basket and the multi-functional insulation tank to swing left and right together with the cell culture bag and the culture fluid therein through the swing frame, causing the culture fluid in the culture bag to flow back and forth in both directions, thus completing the main movement of the cultured cells. Next, because the swing basket assembly is mounted on the swing shaft through a bearing, and the swing basket and the swing shaft rotate relative to each other.

[0062] 2. When the swing rod swings the swing basket to the left, the culture fluid in the culture bag in the swing basket flows to the left, and the center of gravity of the swing basket assembly also moves to the left. At this time, the swing shaft swings to the right around the axis of the load-bearing shaft under the action of this force, and a swing angle is generated with the vertical line, that is, the rightward swing angle. As the swing shaft swings to the right, the horizontal height of the swing axis center also increases, that is, the horizontal height h of the entire swing basket assembly also increases (for example, when the swing straight handle is in a vertical position), so that the swing kinetic energy on it is converted into potential energy (the height increases). When the swing rod swings to the left end point, the swing shaft swings to the right to the highest point. At this time, the kinetic energy of the entire swing basket assembly is completely converted into potential energy, and the potential energy reaches the maximum. When the swing rod swings back, that is, to the right, the culture fluid in the culture bag flows to the right, and the center of gravity of the swing basket assembly moves to the right. At the same time, the potential energy also drives the swing shaft to swing back to the left, that is, the potential energy is converted into the swing kinetic energy of the left swing. Then the swing shaft and the swing straight handle swing to the left around the load-bearing shaft. When the swing reaches the highest point on the left, it is also the point with the largest potential energy. At this moment, the potential energy also drives the swing axis to swing back to the right, that is, the potential energy is converted into the kinetic energy of the swing axis to the right. At the same time, the swing rod begins to swing to the left again. Similarly to the above, the swing axis swings to the right end point, and then begins to swing back to the left. At the same time, the potential energy on the swing axis begins to be converted into kinetic energy for swinging to the left. The whole mechanism repeats itself over and over again, and the next left-right swing cycle begins again.

[0063] 3. In the whole movement process of this double swing mechanism, according to the law of conservation of energy, only a few frictions at the rotation of the shaft and the bearing have kinetic energy converted into heat energy. Because the rotation of the shaft and the bearing is well lubricated and the friction coefficient is extremely small, the energy consumption is extremely small. Similarly, because the movement of the single pendulum mechanism is a back-and-forth conversion of swing kinetic energy and potential energy, the energy consumption is very small, and finally it is converted into positive pressure and acts on the ground. Therefore, the double swing mechanism basically has no impact force, shaking and vibration. This completely solves the problem that the existing mainstream swing-type plastic bag cell reactor in the world generates impact force, vibration, shaking and noise when it swings to the left and right endpoints when the volume is large (more than 50 liters), especially when the volume is large (more than 500 liters), it is more serious, and the result is that such reactors with a volume of more than 50 liters are no longer produced. However, this large-volume double swing plastic bag cell reactor of the present application is estimated to have no problems in terms of impact force, shaking, vibration and noise when manufacturing such a reactor with a volume of 10,000 liters without impact force, slight shaking and vibration and slight noise.

[0064] In summary, the present invention adopts a spherical structure swing box, which adopts a spherical mechanism composed of a swing rod short shaft and a thrust joint bearing and its outer frame to achieve linear reciprocating motion, vertical linear movement in the direction of the short shaft axis and three-dimensional rotation; because the swing rod and the swing frame are long, that is, the force arm is very long, the swing box is subjected to very little force during swinging, so the reaction force received by the swing box and the upper part of the support frame is very small, so the vibration and noise are also very small; the elastic swing rod of the present invention has the characteristics of absorbing, storing and releasing energy, so when the weight of the object being swung is very large and the elastic swing rod is subjected to a large reaction force, it can absorb and release the energy well, so the impact and vibration are very small;

[0065] The present invention adopts the double swing principle: the first swing of the double swing refers to the swing of the swinging basket assembly by the swinging rod around the swinging axis; the weight of the swinging basket assembly acts on the swinging axis, and the swinging basket and the swinging axis rotate relative to each other through the bearing; the second swing of the double swing refers to the swinging of the single pendulum system composed of all loads on the swinging axis through the swinging axis, two swinging straight handles and the load-bearing axis around the load-bearing axis. It is this second single swing system that eliminates the impact force, shaking, vibration and noise generated at the left and right swinging end points in the first swing through the mutual conversion of swinging energy and potential energy, so that the entire double swing reactor finally has only the force of the supporting frame on the ground, that is, the positive pressure. Other things such as shaking, vibration and noise are very small and can almost be ignored. Therefore, the double swing plastic bag cell bioreactor of the present invention can stably culture cells on a large scale without impact force, vibration and noise; in terms of the structure and movement principle of the reactor, when the working volume of the reactor reaches 10,000 liters, it can also work stably without generating impact force, vibration, etc.

[0066] The multifunctional edge-wrapped heat preservation tank of the present invention has an edge-wrapped structure on the upper part, so that when the culture fluid flows to the left and right end points, that is, when the impact force is the largest, the stainless steel edge-wrapped structure bears the impact force, so that the cell culture bag can be protected, and the edge-wrapped structure also increases the heat preservation area of ​​the cell culture bag;

[0067] The two ends of the cell culture bag with an elliptical cross-section of the present invention adopt an elliptical cross-section. First, when the culture bags have the same width, their cross-sectional area and volume are large. Second, when the culture liquid swings left and right to one end, the elliptical cross-section makes the culture bag evenly stressed and will not cause damage caused by stress concentration. In addition, the two welding seams of the culture bag are changed from the left and right ends to the upper and lower oblique symmetry, which also allows the welding seams to avoid the places with the greatest impact force, thereby improving the reliability of the culture bag. Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, not to limit it; although the present invention has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A large-volume double-swing plastic bag cell bioreactor, characterized in that, it includes: a swing box assembly (100), a support frame assembly (200), an elastic swing rod assembly (300), a swing basket assembly (400), a multi-functional edge-wrapped heat preservation tank (500) and a cell culture bag (600); the swing box assembly (100) is installed on the support frame assembly (200); the swing box assembly (100) is connected to the upper part of the swing basket assembly (400) through the elastic swing rod assembly (300); both ends of the load-bearing shaft (800) of the support frame assembly (200) are connected with two swing straight handles (210), and the two swing straight handles (210) are connected through the swing shaft (900) at the bottom of the swing basket assembly (400); the multi-functional edge-wrapped heat preservation tank (500) is arranged in the swing basket assembly (400), and the cell culture bag (600) is arranged in the multi-functional edge-wrapped heat preservation tank (500); the swing box assembly (100) includes: a swing box cover (101), a swing box body (102), a swing rod short shaft (112), a thrust joint bearing combined spherical mechanism (110), a linear guide rail (104), a ball screw (106), a stepping motor (107), a box body bottom plate (108) and a flange plate (109); the swing box cover (101) is connected to the swing box body (102) through the flange plate (109); the stepping motor (107) is arranged on the box body bottom plate (108) of the swing box body (102); the stepping motor (107) is connected to the screw rod of the ball screw (106), the nut of the ball screw (106) is connected to the thrust joint bearing combined spherical mechanism (110), the thrust joint bearing combined spherical mechanism (110) is slidably installed on the linear guide rail (104), the linear guide rail (104) is installed on the box body bottom plate (108), and the thrust joint bearing combined spherical mechanism (110) is penetrated with the swing rod short shaft (112); the bottom plate of the swing box body (102) is provided with a hollowed-out part along the direction of the linear guide rail (104); The support frame assembly (200) includes: a top frame beam (202), two end brackets (204), a swing straight shank bearing (206), a swing straight shank (210), a load-bearing shaft (800), a load-bearing shaft bearing (806), two end inclined support plates (222), a swing bearing (902), and a base bottom plate (208); the top frame beam (202) is connected to the base bottom plate (208) through the two end brackets (204); the two end brackets (204) are respectively provided with two end inclined support plates (222) on their sides; both ends of the load-bearing shaft (800) are connected to the two end brackets (204) through the load-bearing shaft bearings (806); the upper ends of the two swing straight shanks (210) are both connected to the load-bearing shaft (800) through the swing straight shank bearings (206); the lower ends of the two swing straight shanks (210) are both connected to a swing shaft (900) through the swing bearings (902).

2. The large-volume double-swing plastic bag cell bioreactor according to claim 1, wherein, the thrust joint bearing combined spherical mechanism (110) includes: a housing (118), a bushing (116), and a thrust joint bearing (114); the upper and lower ends of the housing (118) are respectively provided with thrust joint bearings (114); the bushing (116) is located between the upper and lower thrust joint bearings (114); the swing rod short shaft (112) is sequentially inserted into the upper thrust joint bearing (114), the bushing (116), and the lower thrust joint bearing (114).

3. The large-volume double-swing plastic bag cell bioreactor according to claim 1, wherein, the elastic swing rod assembly (300) includes: a swing rod (302), an upper disc (304), four tension springs (306), a swing rod horizontal shaft (308), a swing rod horizontal shaft bearing (309), a tension spring fixing ring (320), and a lower disc (310); the upper part of the swing rod (302) passes through the central hole of the upper disc (304) and is connected to the swing rod short shaft (112) of the swing box assembly (100); the lower part of the swing rod (302) is connected to the swing rod horizontal shaft (308); the swing rod horizontal shaft (308) is installed on the lower disc (310) through the swing rod horizontal shaft bearing (309); the upper ends of the four tension springs (306) are respectively connected to the four corners of the upper disc (304); the lower ends of the four tension springs (306) are respectively connected to the periphery of the lower disc (310) through the tension spring fixing rings (320); the lower disc (310) is connected to the swing frame (402) of the swing basket assembly (400).

4. The large-volume double-swing plastic bag cell bioreactor according to claim 3, wherein, a rubber pad is provided at the bottom of the base bottom plate (208).

5. The large-volume double-swing plastic bag cell bioreactor according to claim 4, wherein, the swing basket assembly (400) includes a swing frame (402) and a swing shaft (900); The upper part of the swing frame (402) is connected to the lower disc (310) of the elastic pendulum rod assembly (300); a swing shaft (900) is arranged at the bottom of the swing frame (402).

6. The large-volume double-swing plastic bag cell bioreactor according to claim 5, characterized in that the multi-functional edge-wrapping heat preservation tank (500) is connected to the swing frame (402) of the swing basket assembly (400) through the two end fixing platforms (508); the upper part of the multi-functional edge-wrapping heat preservation tank (500) has an edge-wrapping structure that shrinks inward.

7. The large-volume double-swing plastic bag cell bioreactor according to claim 6, characterized in that the multi-functional edge-wrapping heat preservation tank (500) is heated by a rubber plate or a water jacket.

8. The large-volume double-swing plastic bag cell bioreactor according to claim 1, characterized in that the cross-section of the welded seals (606) at both ends of the cell culture bag (600) is set to be oval; the plastic film seam (602) of the cell culture bag (600) is arranged at the position of its upper and lower diagonals.

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