An automatic sample adding device

Abstract

The present invention discloses a fully automatic sample adding device, comprising: a reagent needle mechanism, a sample adding needle mechanism, a supply device for providing extraction strips, PCR tubes and reagents, a gantry mechanism and a control device; the reagent needle mechanism and the sample adding needle mechanism are both connected to the gantry mechanism, and the gantry mechanism can move horizontally along the supply device; the reagent needle mechanism, the sample adding needle mechanism and the gantry mechanism are all connected to the control device. This device can automatically complete the extraction and addition operations of sample liquid and reagents, effectively reducing the labor intensity of operators and being beneficial to improving operation efficiency and operation accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular diagnosis, and more specifically, to a fully automatic sample adding device. Background Art

[0002] When performing molecular diagnosis, it is necessary to extract nucleic acids and then perform quantitative analysis through an instrument. In an automatic extraction and amplification molecular diagnosis device, the instrument needs to stably extract nucleic acids. In the prior art, generally, after manually using a pipette to pick up a TIP head, the extraction and transfer operations of reagents and sample solutions are carried out. However, this method results in a large manual operation intensity and cannot achieve automatic nucleic acid detection operations.

[0003] In summary, how to automatically extract various reagents and sample solutions required for nucleic acid detection is an urgent problem to be solved by those skilled in the art at present. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a fully automatic sample adding device, which can automatically complete the extraction and addition operations of sample solutions and reagents, effectively reduce the labor intensity of operators, and is beneficial to improving operation efficiency and operation accuracy.

[0005] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0006] A fully automatic sample adding device includes: a reagent needle mechanism for extracting corresponding reagents and adding the reagents to an extraction strip or a PCR tube, a sample adding needle mechanism for adding a sample solution to the extraction strip and mixing the sample solution, a supply device for providing the extraction strip, the PCR tube, and the reagents, a gantry mechanism, and a control device;

[0007] Both the reagent needle mechanism and the sample adding needle mechanism are connected to the gantry mechanism, and the gantry mechanism can move horizontally along the supply device;

[0008] Both the reagent needle mechanism, the sample adding needle mechanism, and the gantry mechanism are connected to the control device.

[0009] Preferably, the gantry mechanism includes a gantry and a transverse movement device for driving the gantry to move horizontally left and right. The reagent needle mechanism and the sample adding needle mechanism are respectively arranged on both sides of the gantry;

[0010] The extraction strip, the PCR tube, and a reagent bin for placing reagents are all arranged on the top of the supply device. The supply device is provided with a guiding device for restricting the movement track of the gantry. The gantry is connected to the guiding device, and the transverse movement device is connected to the control device.

[0011] Preferably, the guiding device includes a linear slide rail and a slider slidably disposed on the linear guide rail. The gantry is disposed on the slider, and the slider is connected to the transverse movement device;

[0012] Or the guiding device includes a guide sleeve and a guide post embedded in the guide sleeve. The guide post is connected to the transverse movement device, and the gantry is disposed on the guide post.

[0013] Preferably, the reagent needle mechanism includes a reagent needle, a longitudinal movement device for driving the reagent needle to move back and forth, and a reagent lifting device for driving the reagent needle to move up and down. The longitudinal movement device and the reagent lifting device are both connected to the reagent needle, and the longitudinal movement device and the reagent lifting device are both connected to the control device.

[0014] Preferably, a TIP and a PCR cover are provided at the top of the supply device, and the reagent needle can pierce the TIP and the PCR cover.

[0015] Preferably, the position of the sample addition needle mechanism is correspondingly set with the position of the extraction strip. When the sample addition needle mechanism performs an up or down operation, liquid can be extracted or injected into the extraction strip.

[0016] Preferably, the sample addition needle mechanism includes a sample addition needle, a sample addition pump for driving the sample addition needle to extract and inject liquid, a first lifting device for driving the plunger of the sample addition pump to move up and down, and a second lifting device for driving the sample addition needle to move up and down. The sample addition pump and the sample addition needle are in communication;

[0017] The first lifting device is connected to the plunger, the second lifting device is connected to the sample addition needle, and the first lifting device and the second lifting device are both connected to the control device.

[0018] Preferably, the sample addition needle mechanism includes a plurality of the sample addition needles and the sample addition pump. The sample addition pump and the sample addition needles are in one-to-one communication. The number of the sample addition needles is the same as the number of the extraction strips, and the positions of the sample addition needles and the extraction strips are in one-to-one correspondence.

[0019] Preferably, a drip prevention mechanism is further provided on the gantry mechanism. The drip prevention mechanism includes a liquid collecting box for collecting dripping liquid and a driving member for driving the liquid collecting box to move horizontally left and right;

[0020] When the reagent needle mechanism is in the raised state, the driving member can drive the liquid collecting box to move below the reagent needle mechanism. When the sample addition needle mechanism is in the raised state, the driving member can drive the liquid collecting box to move below the sample addition needle mechanism.

[0021] Preferably, it further includes a waste liquid treatment mechanism for collecting waste liquid. The sampling needle mechanism can extract the waste liquid in the extraction strip and move the waste liquid to the waste liquid treatment mechanism.

[0022] Preferably, the waste liquid treatment mechanism includes a waste liquid box for collecting waste liquid, a float switch for detecting whether the capacity of the waste liquid box reaches a preset capacity, and a liquid extraction tube for evacuating the waste liquid in the waste liquid box. Both the float switch and the liquid extraction tube are connected to the control device.

[0023] When using the fully automatic sampling device provided by the present invention, first, the gantry mechanism can be driven to move horizontally along the supply device, so that the reagent needle mechanism moves to a position flush with the reagent warehouse. Then, the reagent needle mechanism is controlled to extract the corresponding reagent. After that, the reagent needle mechanism is controlled to leave the reagent warehouse. Then, the gantry mechanism is controlled to move horizontally, so that the reagent needle mechanism moves to a position flush with the extraction strip. Then, the reagent needle mechanism is controlled to add the reagent into the extraction strip. Such operations are repeated until all the required reagents are injected into the extraction strip;

[0024] Then, the gantry mechanism is controlled to move horizontally, so that the sampling needle mechanism moves to a position aligned with the extraction strip. After that, the sampling needle mechanism is controlled to perform the pumping and injection operation of the sample liquid on the extraction strip, and the sampling needle mechanism is driven to perform repeated lifting operations to perform repeated pumping and injection operations on the extraction strip, so that the sample liquid and the reagent are fully mixed and uniform. After that, the sampling needle mechanism is controlled to leave the extraction strip. Then, the gantry mechanism is controlled to move horizontally, so that the reagent needle mechanism moves to a position flush with the extraction strip. Then, the reagent needle mechanism is controlled to suck the mixed sample liquid. Then, the reagent needle mechanism is controlled to leave the extraction strip. After that, the gantry mechanism is controlled to move horizontally, so that the reagent needle mechanism moves to a position flush with the PCR tube. Then, the reagent needle mechanism is controlled to inject the sample liquid into the PCR tube. Finally, the extraction and addition operations of the sample liquid and the reagent are completed. This process does not require manual participation and can effectively improve the operation efficiency and accuracy.

[0025] In summary, the fully automatic sampling device provided by the present invention can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reducing the labor intensity of the operator and being beneficial to improving the operation efficiency and operation accuracy. Description of the Drawings

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0027] Figure 1Schematic structural diagram of the full-automatic sample adding device provided by the present invention;

[0028] Figure 2 Schematic structural diagram of the full-automatic sample adding device from another perspective;

[0029] Figure 3 Schematic structural diagram of the full-automatic sample adding device from yet another perspective;

[0030] Figure 4 Schematic structural diagram when the anti-drip mechanism moves below the reagent needle mechanism;

[0031] Figure 5 Schematic structural diagram when the anti-drip mechanism moves below the sample adding needle mechanism;

[0032] Figure 6 Schematic structural diagram of the waste liquid treatment mechanism.

[0033] Figures 1 - 6 In which:

[0034] 1 is the reagent needle mechanism, 11 is the reagent needle, 12 is the longitudinal movement device, 121 is the second linear slide rail, 122 is the second synchronous belt drive structure, 123 is the longitudinal movement motor, 13 is the reagent lifting device, 2 is the sample adding needle mechanism, 21 is the sample adding needle, 22 is the sample adding pump, 23 is the first lifting device, 24 is the second lifting device, 25 is the moving plate, 3 is the supply device, 31 is the first linear slide rail, 32 is the TIP, 4 is the gantry mechanism, 41 is the gantry, 42 is the transverse movement device, 421 is the transverse movement motor, 422 is the first synchronous belt drive structure, 5 is the extraction strip, 6 is the PCR tube, 7 is the reagent bin, 71 is the reagent hole, 8 is the anti-drip mechanism, 81 is the liquid collecting box, 82 is the first connecting rod, 83 is the second connecting rod, 84 is the roller, 85 is the fan, 86 is the exhaust duct, 9 is the waste liquid treatment mechanism, 91 is the waste liquid box, 92 is the float switch, 93 is the liquid suction pipe. Detailed implementation manners

[0035] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0036] The core of the present invention is to provide a full-automatic sample adding device, which can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reducing the labor intensity of the operator and being beneficial to improving the operation efficiency and operation accuracy.

[0037] Please refer to Figures 1 to 6 ,Figure 1 Schematic structural diagram of the full-automatic sample adding device provided by the present invention; Figure 2 Schematic structural diagram of the full-automatic sample adding device from another perspective; Figure 3 Schematic structural diagram of the full-automatic sample adding device from yet another perspective; Figure 4 Schematic structural diagram when the anti-dripping mechanism moves to below the reagent needle mechanism; Figure 5 Schematic structural diagram when the anti-dripping mechanism moves to below the sample adding needle mechanism;

[0038] Figure 6 Schematic structural diagram of the waste liquid treatment mechanism.

[0039] This specific embodiment provides a full-automatic sample adding device, including: a reagent needle mechanism 1 for extracting corresponding reagents and adding the reagents to an extraction strip 5 or a PCR tube 6, a sample adding needle mechanism 2 for performing sample liquid suction and injection operations on the extraction strip 5, a supply device 3 for providing the extraction strip 5, the PCR tube 6, and the reagents, a gantry mechanism 4, and a control device; both the reagent needle mechanism 1 and the sample adding needle mechanism 2 are connected to the gantry mechanism 4, and the gantry mechanism 4 can move horizontally along the supply device 3; the reagent needle mechanism 1, the sample adding needle mechanism 2, and the gantry mechanism 4 are all connected to the control device.

[0040] It should be noted that the reagent needle mechanism 1 is responsible for completing the extraction and addition operations of the reagents, the sample adding needle mechanism 2 is responsible for completing the addition and mixing operations of the samples, and the gantry mechanism 4 is responsible for the transfer and positioning of the reagent needle mechanism 1 and the sample adding needle mechanism 2 to achieve corresponding operations in multiple channels synchronously, thereby greatly improving the operation efficiency of the device.

[0041] In the actual operation process, according to the actual situation and actual needs, the shapes, structures, dimensions, materials, positions, etc. of the reagent needle mechanism 1, the sample adding needle mechanism 2, the supply device 3, the gantry mechanism 4, and the control device can be determined.

[0042] When using the full-automatic sample adding device provided by the present invention, first, the gantry mechanism 4 can be driven to move horizontally along the supply device 3 so that the reagent needle mechanism 1 moves to a position flush with the reagent warehouse 7, then the reagent needle mechanism 1 is controlled to extract the corresponding reagent, after that, the reagent needle mechanism 1 is controlled to leave the reagent warehouse 7, and then the gantry mechanism 4 is controlled to move horizontally so that the reagent needle mechanism 1 moves to a position flush with the extraction strip 5, and then the reagent needle mechanism 1 is controlled to add the reagent into the extraction strip 5, and so on, until all the required reagents are injected into the extraction strip 5;

[0043] Then, control the gantry mechanism 4 to move horizontally so that the sampling needle mechanism 2 moves to the position aligned with the extraction strip 5. After that, control the sampling needle mechanism 2 to perform the pumping and injection operation of the sample liquid on the extraction strip 5, and drive the sampling needle mechanism 2 to perform repeated lifting operations to perform repeated pumping and injection operations on the extraction strip 5, so that the sample liquid and the reagent are fully mixed evenly. Then, control the sampling needle mechanism 2 to leave the extraction strip 5. Then, control the gantry mechanism 4 to move horizontally so that the reagent needle mechanism 1 moves to the position flush with the extraction strip 5. Then, control the reagent needle mechanism 1 to aspirate the mixed sample liquid. Then, control the reagent needle mechanism 1 to leave the extraction strip 5. After that, control the gantry mechanism 4 to move horizontally so that the reagent needle mechanism 1 moves to the position flush with the PCR tube 6. Then, control the reagent needle mechanism 1 to inject the sample liquid into the PCR tube 6. Finally, the extraction and addition operations of the sample liquid and the reagent are completed. This process does not require manual participation and can effectively improve the operation efficiency and accuracy.

[0044] In summary, the fully automatic sampling device provided by the present invention can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reducing the labor intensity of the operator and being beneficial to improving the operation efficiency and operation accuracy.

[0045] On the basis of the above embodiment, preferably, the gantry mechanism 4 includes a gantry 41 and a transverse movement device 42 for driving the gantry 41 to move horizontally left and right. The reagent needle mechanism 1 and the sampling needle mechanism 2 are respectively arranged on both sides of the gantry 41. The extraction strip 5, the PCR tube 6, and the reagent storage 7 for placing the reagent are all arranged on the top of the supply device 3. The supply device 3 is provided with a guiding device for restricting the movement track of the gantry 41. The gantry 41 is connected to the guiding device, and the transverse movement device 42 is connected to the control device.

[0046] Preferably, the guiding device includes a linear slide rail and a slider slidably arranged on the linear guide rail. The gantry 41 is arranged on the slider, and the slider is connected to the transverse movement device 42; or the guiding device includes a guide sleeve and a guide post embedded in the guide sleeve. The guide post is connected to the transverse movement device 42, and the gantry 41 is arranged on the guide post.

[0047] It should be noted that since the reagent needle mechanism 1 and the sampling needle mechanism 2 are respectively arranged on the left and right sides of the gantry 41, when the control device drives the transverse movement device 42 to move horizontally, the transverse movement device 42 can drive the gantry 41 to move horizontally, and then drive the reagent needle mechanism 1 and the sampling needle mechanism 2 to move horizontally, so that the reagent needle mechanism 1 and the sampling needle mechanism 2 reach the required positions for corresponding operations.

[0048] For example, a first linear slide 31 can be set on the supply device 3, and the transverse movement device 42 includes a transverse movement motor 421 and a first synchronous belt transmission structure 422. The first synchronous belt transmission structures 422 are respectively set on both sides of the first linear slide 31. When the transverse movement motor 421 rotates, it can drive the synchronous belt of the first synchronous belt transmission structure 422 to rotate. The synchronous belt is provided with a first slider that can slide along the first linear slide 31. The gantry 41 is fixedly connected to the first sliders on both sides. Therefore, when the control device drives the transverse movement motor 421 to operate, it will drive the synchronous belt of the first synchronous belt transmission structure 422 to rotate, so that the first sliding drives the gantry 41 to slide along the first linear slide 31, thereby ensuring that the reagent needle mechanism 1 and the sample adding needle mechanism 2 can reach the required positions for corresponding operations.

[0049] It should also be noted that, in addition to using the traverse motor 421 to drive the synchronous belt, pneumatic or other electric devices can also be used for drive. Furthermore, in addition to using a synchronous belt drive for lateral movement, a rack and pinion or other transmission mechanism can also be used to achieve lateral movement of the gantry 41. Furthermore, in addition to using linear slides for guidance, guide posts, guide sleeves, or other guide mechanisms can also be used. In actual use, the shape, structure, size, and position of the gantry 41, traverse device 42, and guide device can be determined based on actual conditions and actual needs.

[0050] Preferably, the reagent needle mechanism 1 includes a reagent needle 11, a longitudinal movement device 12 for driving the reagent needle 11 to move forward and backward, and a reagent lifting device 13 for driving the reagent needle 11 to move up and down. The longitudinal movement device 12 and the reagent lifting device 13 are both connected to the reagent needle 11, and the longitudinal movement device 12 and the reagent lifting device 13 are both connected to the control device.

[0051] It should be noted that the longitudinal movement device 12 may include a second linear slide 121 arranged along the Y-axis direction, a second synchronous belt transmission structure 122 arranged parallel to the second linear slide 121, and a longitudinal movement motor 123. The longitudinal movement motor 123 is connected to the control device. The synchronous belt of the second synchronous belt transmission structure 122 is provided with a second slider that can slide along the second linear slide 121. The reagent needle 11 is fixedly connected to the second slider. Therefore, when the control device drives the longitudinal movement motor 123 to operate, it will drive the synchronous belt of the second synchronous belt transmission structure 122 to rotate. When the synchronous belt rotates, the second slider will drive the reagent needle 11 to slide along the second linear slide 121, thereby ensuring that the reagent needle 11 can reach the required position for corresponding operation.

[0052] It should also be noted that the reagent lifting device 13 may include a reagent lifting motor, and the reagent lifting motor is connected to the control device. When the control device drives the reagent lifting motor to operate, it can drive the adapter of the reagent needle 11 to pick up the TIP32, thereby realizing the extraction and addition operations of the reagent.

[0053] In the actual operation process, the shape, structure, size, position, etc. of the reagent needle 11, the longitudinal movement device 12, and the reagent lifting device 13 can be determined according to the actual situation and actual needs.

[0054] Preferably, the top of the supply device 3 is provided with a TIP32 and a PCR cover, and the reagent needle 11 can pick up the TIP32 and the PCR cover. Among them, the TIP32 is used to cooperate with the reagent needle 11 to aspirate liquid, and the PCR cover is used to cover and seal the PCR tube 6.

[0055] For example, the transverse movement motor 421 can be used to drive the gantry 41 to move horizontally, so that the reagent needle mechanism 1 is positioned at a position flush with the TIP32. Then, the longitudinal movement motor 123 is controlled to drive the reagent needle mechanism 1 to move back and forth, so that the reagent needle 11 is aligned with the TIP32. After that, the reagent lifting motor is used to control the reagent needle 11 to pick up the TIP32. When the reagent needle 11 successfully picks up the TIP32, the reagent needle 11 is controlled to rise. Then, the transverse movement motor 421 is used to drive the gantry 41 to move horizontally, and the longitudinal movement motor 123 is controlled to drive the reagent needle mechanism 1 to move back and forth, so that the reagent needle mechanism 1 moves to directly above the reagent storage 7. The top of the reagent storage 7 is provided with a reagent hole 71 to facilitate the reagent needle 11 to aspirate the reagent. Then, the reagent lifting motor is used to control the reagent needle 11 to cooperate with the TIP32 to aspirate the reagent. When the reagent needle 11 successfully extracts the reagent, the reagent needle 11 is controlled to rise again. Then, the transverse movement motor 421 is used to drive the gantry 41 to move horizontally, and the longitudinal movement motor 123 is controlled to drive the reagent needle mechanism 1 to move back and forth, so that the reagent needle mechanism 1 moves to directly above the extraction strip 5. Then, the reagent lifting motor is used to control the reagent needle 11 to inject the reagent into the corresponding reaction chamber, and so on until all the required reaction chambers are filled with the corresponding reagent. Similarly, through similar operations, the reagent needle mechanism 1 can be driven to extract the mixed sample liquid and inject the mixed sample liquid into the corresponding PCR tube 6. Moreover, the reagent needle mechanism 1 can also use the PCR cover to seal and cover the PCR tube 6 to facilitate subsequent detection operations on the sealed PCR tube 6 containing the sample liquid.

[0056] In the actual operation process, the shape, structure, size, material, position, etc. of the TIP32, PCR cover, PCR tube 6, and reagent storage 7 on the supply device 3 can be determined according to the actual situation and actual needs.

[0057] On the basis of the above embodiments, preferably, the position of the sampling needle mechanism 2 is correspondingly set with the position of the extraction strip 5. When the sampling needle mechanism 2 performs an ascending or descending operation, liquid extraction or injection operations can be performed on the extraction strip 5.

[0058] Preferably, the sampling needle mechanism 2 includes a sampling needle 21, a sampling pump 22 for driving the sampling needle 21 to extract and inject liquid, a first lifting device 23 for driving the plunger of the sampling pump 22 to move up and down, and a second lifting device 24 for driving the sampling needle 21 to move up and down. The sampling pump 22 and the sampling needle 21 are connected; the first lifting device 23 is connected to the plunger, the second lifting device 24 is connected to the sampling needle 21, and both the first lifting device 23 and the second lifting device 24 are connected to the control device. Therefore, after the control device controls the first lifting device 23 and the second lifting device 24 to descend synchronously, the liquid injection operation of the sample liquid can be realized, and after the control device controls the first lifting device 23 and the second lifting device 24 to ascend synchronously, the liquid extraction operation of the sample liquid can be realized. The repeated extraction and injection process of the sampling needle 21 can effectively mix the sample liquid and various reagents.

[0059] Preferably, the sampling needle mechanism 2 includes a plurality of sampling needles 21 and sampling pumps 22. The sampling pumps 22 and the sampling needles 21 are connected in one-to-one correspondence. The number of sampling needles 21 is the same as the number of extraction strips 5, and the positions of the sampling needles 21 and the extraction strips 5 are in one-to-one correspondence. For example, 8 sampling needles 21 can be arranged side by side, and the 8 sampling needles 21 and 8 extraction strips 5 are in one-to-one correspondence, so that the device structure is more compact, occupies less instrument space, and can realize multi-channel synchronous operation to effectively shorten the sampling time and improve the operation efficiency.

[0060] For example, a transverse movement motor 421 can be used to drive the gantry 41 to move horizontally to position the sampling needle mechanism 2 above the extraction strip 5, and then the first lifting device 23 and the second lifting device 24 are used to drive the sampling needle mechanism 2 to extract the sample liquid in the extraction strip 5. Then, the transverse movement motor 421 is used to drive the gantry 41 to move horizontally to move the sampling needle mechanism 2 to a position above the reaction chamber of the extraction strip 5, and then inject the sample liquid into the corresponding reaction chamber. Repeat the above operations until all the required reaction chambers are injected with the corresponding sample liquid and mixed.

[0061] In the actual application process, the shapes, structures, dimensions, positions, etc. of the sampling needle 21, the sampling pump 22, the first lifting device 23, and the second lifting device 24 can be determined according to the actual situation and actual needs.

[0062] On the basis of the above embodiments, preferably, it further includes a liquid dripping prevention mechanism 8 provided on the gantry mechanism 4. The liquid dripping prevention mechanism 8 includes a liquid collecting box 81 for collecting dripping liquid and a driving member for driving the liquid collecting box 81 to move horizontally left and right; when the reagent needle mechanism 1 is in the rising state, the driving member can drive the liquid collecting box 81 to move below the reagent needle mechanism 1, and when the sample adding needle mechanism 2 is in the rising state, the driving member can drive the liquid collecting box 81 to move below the sample adding needle mechanism 2.

[0063] It should be noted that the sample adding needle mechanism 2 and the reagent needle mechanism 1 work alternately. After a certain mechanism moves upward, it needs to drive the corresponding liquid to move in space. Therefore, it is necessary to set a liquid collecting box 81 below this mechanism to effectively reduce liquid dripping and avoid pollution of the equipment. Each sample adding needle 21 can be fixed on the same moving plate 25. When the sample adding needle 21 performs lifting and lowering operations, the moving plate 25 will move up and down synchronously.

[0064] Preferably, the driving member may include: a sliding plate, a fixing plate, a fixing block fixedly provided on the gantry 41, a first connecting rod 82 hinged to the fixing block, a second connecting rod 83 hinged to the first connecting rod 82, and a sliding block hinged to the second connecting rod 83. The sliding block is provided on the sliding plate. The first connecting rod 82 and the second connecting rod 83 are hinged and fixed through the rotating shaft of the roller 84. The liquid collecting box 81 is fixedly connected to the sliding plate, and the liquid collecting box 81 is arranged parallel to the lower side of the sliding plate. The fixing plate is provided with a slide rail for slidingly cooperating with the sliding plate, as Figure 4 and Figure 5 shown.

[0065] When the moving plate 25 moves downward to the position in contact with the driving member, as the moving plate 25 continues to move downward, the moving plate 25 will exert a leftward force on the roller 84. When the roller 84 is subjected to an external force horizontally to the left and moves to the left, the first connecting rod 82 and the second connecting rod 83 will push the sliding block to the left through the action of the rotating shaft. The sliding block can drive the sliding plate to move to the left together. Since the liquid collecting box 81 is fixed below the sliding plate, therefore, the sliding plate can drive the liquid collecting box 81 to move synchronously to the left. At this time, the left liquid collecting groove of the liquid collecting box 81 is directly below the reagent needle mechanism 1 on the left side of the gantry 41 to prevent reagent dripping from occurring in the reagent needle mechanism 1 during the moving process.

[0066] When the moving plate 25 runs upward, after the moving plate 25 is separated from the driving member, that is, after the external force on the roller 84 is removed, the elastic member can move the sliding plate to the right to drive the liquid holding box 81 to move to the right. At this time, the right liquid holding groove of the liquid holding box 81 is directly below the sampling needle mechanism 2 on the right side of the gantry 41 to prevent the reagent from dripping from the sampling needle mechanism 2 during the moving process. The device uses the elastic member and the linkage mechanism to realize the extension and avoidance operations of the liquid holding box 81, so as to ensure the smooth progress of the extension and retraction operations of the liquid holding box 81 and make the left and right position switching of the liquid holding box 81 stable. In addition, a fan 85 and an exhaust duct 86 can be provided to discharge the reagent aerosol on both sides of the liquid holding box 81 outward to avoid the aerosol from polluting the equipment.

[0067] In the actual application process, the shapes, structures, dimensions, positions, etc. of the liquid holding box 81, the driving member, and the moving plate 25 can be determined according to the actual situation and actual needs.

[0068] Preferably, it further includes a waste liquid treatment mechanism 9 for collecting waste liquid. The sampling needle mechanism 2 can extract the waste liquid in the extraction strip 5 and move the waste liquid to the waste liquid treatment mechanism 9.

[0069] Preferably, the waste liquid treatment mechanism 9 includes a waste liquid box 91 for collecting waste liquid, a float switch 92 for detecting whether the capacity of the waste liquid box 91 reaches a preset capacity, and a liquid extraction tube 93 for evacuating the waste liquid in the waste liquid box 91. The float switch 92 and the liquid extraction tube 93 are both connected to the control device.

[0070] It should be noted that after the sample liquid in the extraction strip 5 is transferred to the PCR tube 6 for detection, there will still be some liquid remaining in the extraction strip 5. At this time, the sampling needle mechanism 2 can be used to suck the remaining waste liquid and transfer the waste liquid to the waste liquid treatment mechanism 9. When the amount of waste liquid in the waste liquid box 91 reaches the set value, the float switch 92 will be triggered. The float switch 92 will feedback the signal to the control device, and the control device can start the liquid extraction tube 93 to pump out the waste liquid to complete the cleaning work of the remaining waste liquid, effectively improving the stability and safety of the equipment.

[0071] In the actual application process, the shapes, structures, dimensions, positions, etc. of the waste liquid box 91, the float switch 92, and the liquid extraction tube 93 can be determined according to the actual situation and actual needs.

[0072] It should also be noted that the fully automatic sampling device provided by the present invention can realize automatic sampling operations, effectively preventing contamination caused by manual intervention. Moreover, the device is provided with a corresponding anti-dripping mechanism 8 and a waste liquid treatment mechanism 9, effectively improving the use effect, structural stability, and safety of the device, and can be popularized and used.

[0073] It should be noted that for the first lifting device 23 and the second lifting device 24, the first synchronous belt drive structure 422 and the second synchronous belt drive structure 122, the first linear slide rail 31 and the second linear slide rail 121, the first slider and the second slider mentioned in this application document, where the first and the second are only used to distinguish different positions and there is no order of precedence.

[0074] In addition, it should also be noted that the orientation or positional relationship indicated by "up and down", "front and back", "left and right", etc. in this application is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of simplifying the description and understanding, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0075] The various embodiments in this specification are described in a progressive manner. Each embodiment focuses on the differences from other embodiments. The same or similar parts among the various embodiments can be referred to each other. Any combination of all the embodiments provided by the present invention is within the protection scope of this invention and will not be elaborated here.

[0076] The above has introduced the fully automatic sample adding device provided by the present invention in detail. Specific examples are used in this article to elaborate on the principle and implementation manner of the present invention. The description of the above embodiments is only used to help understand the method and its core idea of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. An automatic sample adding device, characterized in that, Including: A reagent needle mechanism (1) for extracting corresponding reagents and adding the reagents to an extraction strip (5) or a PCR tube (6), a sampling needle mechanism (2) for performing a sample liquid pumping and injection operation on the extraction strip (5), a supply device (3) for providing the extraction strip (5), the PCR tube (6), and the reagents, a gantry mechanism (4), and a control device; Both the reagent needle mechanism (1) and the sampling needle mechanism (2) are connected to the gantry mechanism (4), and the gantry mechanism (4) can move horizontally along the supply device (3); The reagent needle mechanism (1), the sampling needle mechanism (2), and the gantry mechanism (4) are all connected to the control device; It further includes a drip prevention mechanism (8) provided on the gantry mechanism (4). The drip prevention mechanism (8) includes a liquid collecting box (81) for collecting dripping liquid and a driving member for driving the liquid collecting box (81) to move horizontally. When the reagent needle mechanism (1) is in the rising state, the driving member drives the liquid collecting box to move below the reagent needle mechanism (1). When the sampling needle mechanism (2) is in the rising state, the driving member drives the liquid collecting box to move below the sampling needle mechanism (2). The drip prevention mechanism (8) further includes a fan (85) and an exhaust duct (86) to exhaust the reagent aerosol on both sides of the liquid collecting box (81) outward; Each sampling needle (21) of the sampling needle mechanism (2) is fixed on the same moving plate (25). The driving member includes a sliding plate, a fixing plate, a fixed block fixedly provided on the gantry mechanism (4), a first connecting rod (82) hinged to the fixed block, a second connecting rod (83) hinged to the first connecting rod (82), a sliding block hinged to the second connecting rod (83), and an elastic member for driving the sliding plate to reset. The sliding block is provided on the sliding plate. The first connecting rod (82) and the second connecting rod (83) are hinged and fixed through the rotating shaft of a roller (84). The liquid collecting box (81) is fixedly connected to the sliding plate, and the liquid collecting box (81) is arranged parallel to the lower side of the sliding plate. The fixing plate is provided with a slide rail for slidingly cooperating with the sliding plate; The sampling needle mechanism (2) includes a plurality of the sampling needles (21) and a sampling pump (22). The sampling pump (22) and the sampling needles (21) are in one-to-one correspondence and communication. The number of the sampling needles (21) is the same as the number of the extraction strips (5), and the positions of the sampling needles (21) and the positions of the extraction strips (5) are in one-to-one correspondence. When the sampling needle mechanism (2) performs a rising or falling operation, a liquid pumping or injection operation can be performed on the extraction strip (5); A first linear slide rail (31) is provided on the supply device (3). The gantry mechanism (4) includes a transverse movement motor (421) and a first synchronous belt drive structure (422). The first synchronous belt drive structures (422) are respectively arranged on both sides of the first linear slide rail (31). When the transverse movement motor (421) rotates, it drives the synchronous belt of the first synchronous belt drive structure (422) to rotate. A first slider that slides along the first linear slide rail (31) is provided on the synchronous belt, and the gantry mechanism (4) is fixedly connected to the first sliders on its both sides.

2. The full-automatic sampling device according to claim 1, wherein The gantry mechanism (4) includes a gantry (41) and a transverse movement device (42) for driving the gantry (41) to move horizontally. The reagent needle mechanism (1) and the sample addition needle mechanism (2) are respectively arranged on both sides of the gantry (41). The extraction strip (5), the PCR tube (6), and the reagent bin (7) for placing reagents are all arranged on the top of the supply device (3). The supply device (3) is provided with a guiding device for restricting the movement track of the gantry (41). The gantry (41) is connected to the guiding device, and the transverse movement device (42) is connected to the control device.

3. The fully automatic sample adding device according to claim 2, wherein The guiding device includes a linear slide rail and a slider that slides on the linear guide rail. The gantry (41) is arranged on the slider, and the slider is connected to the transverse movement device (42). Or the guiding device includes a guide sleeve and a guide post embedded in the guide sleeve. The guide post is connected to the transverse movement device (42), and the gantry (41) is arranged on the guide post.

4. The fully automatic sampling device according to claim 1, wherein, The reagent needle mechanism (1) includes a reagent needle (11), a longitudinal movement device (12) for driving the reagent needle (11) to move back and forth, and a reagent lifting device (13) for driving the reagent needle (11) to move up and down. The longitudinal movement device (12) and the reagent lifting device (13) are both connected to the reagent needle (11), and the longitudinal movement device (12) and the reagent lifting device (13) are both connected to the control device.

5. The fully automatic sampling device according to claim 4, wherein TIPs (32) and PCR caps are provided on the top of the supply device (3), and the reagent needle (11) can pierce and pick up the TIPs (32) and the PCR caps.

6. The fully automatic sampling device according to any one of claims 1 to 5, characterized in that, The sample addition needle mechanism (2) further includes a first lifting device (23) for driving the plunger of the sample addition pump (22) to move up and down and a second lifting device (24) for driving the sample addition needle (21) to move up and down. The sample addition pump (22) and the sample addition needle (21) are communicated with each other. The first lifting device (23) is connected to the plunger, the second lifting device (24) is connected to the sample addition needle (21), and the first lifting device (23) and the second lifting device (24) are both connected to the control device.

7. The full-automatic sample adding device according to any one of claims 1 to 5, characterized in that, It further includes a waste liquid treatment mechanism (9) for collecting waste liquid. The sample addition needle mechanism (2) can extract the waste liquid in the extraction strip (5) and move the waste liquid to the waste liquid treatment mechanism (9).

8. The fully automatic sample adding device according to claim 7, wherein, The waste liquid treatment mechanism (9) includes a waste liquid box (91) for collecting waste liquid, a float switch (92) for detecting whether the capacity of the waste liquid box (91) reaches a preset capacity, and a liquid extraction pipe (93) for evacuating the waste liquid in the waste liquid box (91). The float switch (92) and the liquid extraction pipe (93) are both connected to the control device.

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