Composition containing gamma-aminobutyric acid and its application

Abstract

The present invention discloses a composition containing γ-aminobutyric acid and its application. The composition containing γ-aminobutyric acid of the present invention comprises γ-aminobutyric acid and N-acetyl glucosamine. The composition provided by the present invention can effectively alleviate the immediate discomfort caused by the use of γ-aminobutyric acid, especially the tingling sensation, through the synergistic compounding of γ-aminobutyric acid and N-acetyl glucosamine. The composition has simple ingredients and preparation, and the raw materials are easily available, the cost is low, and it is easy to promote and commercialize.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition containing gamma-aminobutyric acid and application thereof. Background Art

[0002] Gamma-aminobutyric acid (GABA) is a natural amino acid that is widely distributed in animals and plants. It has a relatively small molecular weight and can quickly penetrate the epidermis, relax tense muscles, significantly smooth fine lines, and make the skin smooth and firm. At the same time, GABA can promote the proliferation of epidermal cells and the production of keratinocytes to repair the stratum corneum. Using this characteristic, many anti-aging products have been developed at home and abroad, such as facial masks, eye creams, etc.

[0003] In commercial products, the amount of GABA used ranges from 0.3-5.0%. When the concentration of GABA reaches a certain amount, some consumers will experience discomfort, especially a tingling sensation, and this sensation increases with the increase in GABA concentration.

[0004] Patent application 201510943689.6 discloses a composition containing γ-aminobutyric acid and hyaluronic acid and its application in cosmetics. The composition includes γ-aminobutyric acid and hyaluronic acid or its salt as active ingredients, which can significantly improve and reduce the adverse reactions of γ-aminobutyric acid to the skin, and overcome the irritation to the skin caused by using γ-aminobutyric acid alone. The composition requires high and low molecular weight hyaluronic acid to be compounded with γ-aminobutyric acid in a certain mass ratio to play a role in reducing irritation. The preparation process is complicated and the cost is high. Summary of the invention

[0005] In view of the above problems, the present invention provides a composition containing γ-aminobutyric acid and its application. The inventors creatively discovered that N-acetyl glucosamine (NAG) can effectively reduce the irritation of γ-aminobutyric acid and can be widely used in cosmetics containing γ-aminobutyric acid.

[0006] The present invention can effectively alleviate the immediate discomfort, especially the tingling sensation, caused by the use of the gamma-aminobutyric acid by synergistically compounding N-acetyl glucosamine and gamma-aminobutyric acid.

[0007] The specific technical solutions of the present invention are as follows:

[0008] 1. A composition containing γ-aminobutyric acid, comprising γ-aminobutyric acid and N-acetyl glucosamine.

[0009] 2. The composition according to item 1, wherein the amount of the γ-aminobutyric acid is 0.1-5 parts, preferably 0.3-5 parts, and more preferably 2-4 parts, based on 100 parts by weight of the composition containing γ-aminobutyric acid;

[0010] The N-acetyl glucosamine is 0.1-10 parts, preferably 0.5-5 parts, and more preferably 3-5 parts.

[0011] 3. According to the composition of item 1, the mass ratio of the γ-aminobutyric acid to the N-acetyl glucosamine is 1:0.1-4, preferably 1:1-2.

[0012] 4. A cosmetic comprising the composition according to any one of items 1 to 3.

[0013] 5. The cosmetic according to item 4, wherein the cosmetic further comprises auxiliary materials.

[0014] 6. The cosmetic according to item 4 or 5, wherein the cosmetic is an anti-wrinkle and anti-fine line product, and preferably, the cosmetic is in the form of a cream, lotion, mask or essence.

[0015] 7. The cosmetic according to any one of items 4 to 6, wherein the sum of the mass of γ-aminobutyric acid and N-acetyl glucosamine in the composition accounts for 1-10%, preferably 2-6%, by weight percentage in the cosmetic.

[0016] 8. A method for using cosmetics, comprising applying the cosmetics according to any one of items 4 to 7 to a desired area of ​​a user.

[0017] 9. Use of the composition according to any one of items 1 to 3 in the field of cosmetics.

[0018] 10. The use according to item 9, wherein the cosmetic is an anti-wrinkle and anti-fine line product, and preferably, the cosmetic is in the form of a cream, lotion, mask or essence.

[0019] Effects of the Invention

[0020] 1. The composition provided by the present invention can effectively alleviate the immediate discomfort, especially the tingling sensation, caused by the use of γ-aminobutyric acid by synergistically compounding γ-aminobutyric acid and N-acetyl glucosamine.

[0021] 2. The composition for alleviating γ-aminobutyric acid stimulation provided in the present application has simple ingredients and preparation, readily available raw materials, low cost, and is easy to promote and commercialize. DETAILED DESCRIPTION

[0022] The present invention is described in detail below. Although specific embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0023] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the difference in the functions of the components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" are open-ended terms and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present invention, but the description is based on the general principles of the specification and is not intended to limit the scope of the present invention. The scope of protection of the present invention shall be determined by the attached claims.

[0024] The invention provides a composition containing gamma-aminobutyric acid, which comprises gamma-aminobutyric acid and N-acetyl glucosamine.

[0025] The γ-aminobutyric acid is a natural amino acid widely distributed in animals and plants. It has a relatively small molecular weight and can quickly penetrate the epidermis, relax tense muscles, significantly smooth fine lines, and make the skin smooth and firm. At the same time, γ-aminobutyric acid can promote the proliferation of epidermal cells and the production of keratinocytes to repair the stratum corneum. After human testing, it has the effect of instantly soothing fine lines.

[0026] N-acetyl glucosamine, also known as N-acetyl-D-glucosamine (NAG), is the basic component of many important polysaccharides in biological cells and has many important physiological functions in organisms. NAG is a small molecule amino monosaccharide obtained through biological fermentation. It has excellent transdermal absorption and can improve skin hydration. It is a high-quality multifunctional moisturizer and is widely used in various cosmetic formula systems. A large number of literature and patents show that it has moisturizing and whitening effects, and has been used in market products for many years.

[0027] The present invention can effectively alleviate the immediate discomfort, especially the tingling sensation, caused by the use of the gamma-aminobutyric acid by synergistically compounding the gamma-aminobutyric acid with N-acetyl glucosamine.

[0028] In one embodiment, the composition containing γ-aminobutyric acid is composed of γ-aminobutyric acid, N-acetyl glucosamine and other bases and auxiliary materials, such as water.

[0029] In one embodiment, based on 100 parts by weight of the composition containing γ-aminobutyric acid, the γ-aminobutyric acid is 0.1-5 parts, preferably 0.3-5 parts, and more preferably 2-4 parts;

[0030] The N-acetyl glucosamine is 0.1-10 parts, preferably 0.5-5 parts, and more preferably 3-5 parts.

[0031] For example, based on 100 parts by weight of the composition containing γ-aminobutyric acid, the γ-aminobutyric acid may be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.;

[0032] The N-acetyl glucosamine can be 0.1 parts, 0.5 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0033] In one embodiment, the mass ratio of the γ-aminobutyric acid to the N-acetyl glucosamine (m γ-氨基丁酸 :m N-乙酰壳糖胺 ) is 1:0.1-4, preferably 1:1-2.

[0034] For example, the mass ratio of the γ-aminobutyric acid to the N-acetyl glucosamine (m γ-氨基丁酸 :m N-乙酰壳糖胺 ) can be 1:0.1, 1:1, 1:1.5, 1:2, 1:3, 1:4, etc.

[0035] The present invention provides a cosmetic comprising the above composition.

[0036] By using the above-mentioned composition, it is possible to reduce the discomfort, especially the stinging sensation, of cosmetics containing GABA.

[0037] In one embodiment, the cosmetic further comprises auxiliary materials, which are auxiliary materials known to those skilled in the art, for example, the auxiliary materials may be: oils, emulsifiers, thickeners, preservatives, and the like.

[0038] The present invention does not impose any limitation on cosmetics. For example, the cosmetics may be anti-wrinkle and anti-fine line products, and the dosage forms of the cosmetics may be creams, lotions, essences, facial masks, and the like.

[0039] The present invention does not impose any limitation on the weight percentage of the composition in the cosmetic, which can be adjusted as needed. For example, the sum of the weight of γ-aminobutyric acid and N-acetyl glucosamine in the composition can account for 1-10%, preferably 2-6%, based on the weight percentage in the cosmetic.

[0040] For example, in terms of weight percentage in the cosmetic, the sum of the mass of γ-aminobutyric acid and N-acetyl glucosamine in the composition can account for 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0041] The present invention provides a method for using cosmetics, comprising applying the above-mentioned cosmetics to the desired part of the user.

[0042] For example, the cosmetics can be applied to the user's face, the user's nose, etc., which can be determined according to the needs of the user.

[0043] The present invention provides the use of the above-mentioned composition in the field of cosmetics. Preferably, the cosmetics can be anti-wrinkle and anti-fine line products. More preferably, the dosage form of the cosmetics is cream, lotion, mask or essence.

[0044] The invention can reduce the discomfort, especially the stinging feeling, caused by consumers using cosmetics containing a certain amount of gamma-aminobutyric acid by compounding gamma-aminobutyric acid and N-acetyl glucosamine.

[0045] Example

[0046] The present invention generally and / or specifically describes the materials and test methods used in the test. In the following examples, unless otherwise specified, % represents wt%, i.e., weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be obtained commercially, among which γ-aminobutyric acid and N-acetyl glucosamine were purchased from Bloomage Biotechnology Co., Ltd.

[0047] Example 1

[0048] Put 4 g of γ-aminobutyric acid, 4 g of N-acetyl glucosamine and 92 g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0049] Example 2

[0050] 4 g of γ-aminobutyric acid, 5 g of N-acetyl glucosamine and 91 g of water were placed in a beaker, heated to 65° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0051] Example 3

[0052] Put 3g of γ-aminobutyric acid, 3g of N-acetyl glucosamine and 94g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0053] Example 4

[0054] 3 g of γ-aminobutyric acid, 0.5 g of N-acetyl glucosamine and 96.5 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0055] Example 5

[0056] Put 2g of γ-aminobutyric acid, 3g of N-acetyl glucosamine and 95g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0057] Example 6

[0058] 0.3 g of γ-aminobutyric acid, 0.5 g of N-acetyl glucosamine and 99.2 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0059] Example 7

[0060] 3.5 g of γ-aminobutyric acid, 5 g of N-acetyl glucosamine and 91.5 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0061] Example 8

[0062] 0.1 g of γ-aminobutyric acid, 0.1 N-acetyl glucosamine and 99.8 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0063] Example 9

[0064] Put 5g of γ-aminobutyric acid, 10g of N-acetyl glucosamine and 85g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0065] Example 10

[0066] Put 5g of γ-aminobutyric acid, 8g of N-acetyl glucosamine and 87g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0067] Embodiment 11

[0068] 5 g of γ-aminobutyric acid, 12 g of N-acetyl glucosamine and 83 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0069] Example 12

[0070] Put 3g of γ-aminobutyric acid, 5g of N-acetyl glucosamine and 92g of water into a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0071] Example 13

[0072] 3 g of γ-aminobutyric acid, 10 N-acetyl glucosamine and 87 g of water were placed in a beaker, heated to 60° C. with stirring until completely dissolved, and then cooled to room temperature for later use.

[0073] Comparative Example 1

[0074] Place 4 g of γ-aminobutyric acid and 96 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0075] Comparative Example 2

[0076] Place 3 g of γ-aminobutyric acid and 97 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0077] Comparative Example 3

[0078] Place 2 g of γ-aminobutyric acid and 98 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0079] Comparative Example 4

[0080] Place 0.3 g of γ-aminobutyric acid and 99.7 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0081] Comparative Example 5

[0082] Place 3.5 g of γ-aminobutyric acid and 96.5 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0083] Comparative Example 6

[0084] Place 0.1 g of γ-aminobutyric acid and 99.9 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0085] Comparative Example 7

[0086] Place 5 g of γ-aminobutyric acid and 95 g of water in a beaker, stir and heat to 60°C until completely dissolved, then cool to room temperature for later use.

[0087] Table 1 Dosage table of components used in Examples and Comparative Examples

[0088]

[0089]

[0090] Test example: Single use sensory test

[0091] The test was conducted with reference to the method of “Study on the Evaluation Method for the Suitability of Facial Cosmetics for Sensitive Skin” (Ma Li, Tan Yimei, Cheng Ying, et al., Study on the Evaluation Method for the Suitability of Facial Cosmetics for Sensitive Skin, Chinese Journal of Integrated Traditional and Western Medicine Dermatology and Venereology, Vol. 17, No. 1, 2018).

[0092] The subjects who met the selection criteria (discomfort with GABA) were screened and tested one week later. A total of 35 subjects were selected. The sample was dropped on a filter paper with a diameter of 8 mm and placed on the nasolabial grooves on both sides of the subject. The control sample was used on one side and the embodiment was used on the other side. The subjects evaluated the discomfort degree of itching, tingling and burning pain in the test site at 30s, 2.5min, 5min and 10min according to the four-point method (0 for no feeling, 1 for mild, 2 for moderate, and 3 for severe). The results are shown in Tables 2 to 12, respectively, where the tingling score is as follows:

[0093] Pain score = (0min + 0.5min + 2.5min + 5min + 10min pain score on the control side) - (0min + 0.5min + 2.5min + 5min + 10min pain score on the example side)

[0094] The Wilcoxon signed order test method was used for statistical analysis. The level was set at a = 0.05 to test that the mean was in a beneficial direction, and the test result p < 0.05 indicated that it was effective.

[0095] Results indicate: ***: p<0.001; **: p<0.01; *: p<0.05; nd: p>0.05

[0096] Table 2 Score of stinging sensation at each time point of Example 1 and Comparative Example 1

[0097] 0min 0.5min 2.5min 5min 10min sum Example 1 (left) 0 <![CDATA[0.14 * ]]> <![CDATA[0.23 * ]]> <![CDATA[0.29 ** ]]> <![CDATA[0.31 ** ]]> 0.97 Comparative Example 1 (right side) 0.09 0.34 0.49 0.74 0.71 2.37 Pain score 0.09 0.20 0.26 0.45 0.40 1.40

[0098] Table 3 Score of stinging sensation at each time point of Example 2 and Comparative Example 1

[0099] 0min 0.5min 2.5min 5min 10min sum Example 2 (left) 0 <![CDATA[0.15 * ]]> <![CDATA[0.25 * ]]> <![CDATA[0.30 ** ]]> <![CDATA[0.31 ** ]]> 1.01 Comparative Example 1 (right side) 0.09 0.32 0.47 0.75 0.72 2.35 Pain score 0.09 0.17 0.22 0.45 0.41 1.34

[0100] Table 4 Score of stinging sensation at each time point of Example 3 and Comparative Example 2

[0101] 0min 0.5min 2.5min 5min 10min sum Example 3 (left) 0 0.10 <![CDATA[0.17 * ]]> <![CDATA[0.23 ** ]]> <![CDATA[0.26 ** ]]> 0.76 Comparative Example 2 (right side) 0.04 0.25 0.33 0.64 0.70 1.96 Pain score 0.04 0.15 0.16 0.41 0.44 1.20

[0102] Table 5: Score of stinging sensation at each time point for Example 4 and Comparative Example 2

[0103] 0min 0.5min 2.5min 5min 10min sum Example 4 (left) 0 <![CDATA[0.12 * ]]> 0.20 <![CDATA[0.35 * ]]> 0.38 1.05 Comparative Example 2 (right side) 0.05 0.28 0.34 0.51 0.53 1.71 Pain score 0.05 0.16 0.14 0.16 0.15 0.66

[0104] Table 6 Score of stinging sensation at each time point of Example 5 and Comparative Example 3

[0105] 0min 0.5min 2.5min 5min 10min sum Example 5 (left) 0 0.08 <![CDATA[0.13 * ]]> <![CDATA[0.20 ** ]]> <![CDATA[0.23 * ]]> 0.64 Comparative Example 3 (right side) 0.02 0.18 0.37 0.60 0.62 1.79 Pain score 0.02 0.10 0.24 0.40 0.39 1.15

[0106] Table 7 Score of stinging sensation at each time point of Example 6 and Comparative Example 4

[0107] 0min 0.5min 2.5min 5min 10min sum Example 6 (left) 0 0.02 0.04 0.06 0.08 0.2 Comparative Example 4 (right side) 0 0.04 0.06 0.09 0.10 0.29 Pain score 0 0.02 0.02 0.03 0.02 0.09

[0108] Table 8 Score of stinging sensation at each time point for Example 7 and Comparative Example 5

[0109] 0min 0.5min 2.5min 5min 10min sum Example 7 (left) 0 0.15 <![CDATA[0.28 * ]]> <![CDATA[0.26 ** ]]> <![CDATA[0.29 ** ]]> 0.98 Comparative Example 5 (right side) 0.07 0.30 0.45 0.73 0.72 2.27 Pain score 0.07 0.15 0.17 0.47 0.43 1.29

[0110] Table 9 Score of stinging sensation at each time point for Example 8 and Comparative Example 6

[0111] 0min 0.5min 2.5min 5min 10min sum Example 8 (left) 0 0.02 0.01 0.02 0.01 0.06 Comparative Example 6 (right side) 0 0.02 0.01 0.02 0.02 0.07 Pain score 0 0 0 0 0.01 0.01

[0112] Table 10 Score of stinging sensation at each time point for Example 9 and Comparative Example 7

[0113] 0min 0.5min 2.5min 5min 10min sum Example 9 (left) 0.08 <![CDATA[0.20 * ]]> <![CDATA[0.42 * ]]> <![CDATA[0.59 * ]]> 0.65 1.94 Comparative Example 7 (right side) 0.12 0.39 0.60 0.77 0.76 2.64 Pain score 0.04 0.19 0.18 0.18 0.11 0.70

[0114] Table 11 Score of stinging sensation at each time point for Example 10 and Comparative Example 7

[0115] 0min 0.5min 2.5min 5min 10min sum Example 10 (left) 0.09 <![CDATA[0.16 * ]]> <![CDATA[0.37 * ]]> <![CDATA[0.51 * ]]> <![CDATA[0.54 * ]]> 1.67 Comparative Example 7 (right side) 0.11 0.37 0.58 0.74 0.73 2.53 Pain score 0.02 0.21 0.21 0.23 0.19 0.86

[0116] Table 12 Score of stinging sensation at each time point for Example 11 and Comparative Example 7

[0117] 0min 0.5min 2.5min 5min 10min sum Example 11 (left) 0.01 0.15 0.22 <![CDATA[0.35 * ]]> 0.39 1.12 Comparative Example 7 (right side) 0.04 0.29 0.33 0.59 0.52 1.77 Pain score 0.03 0.14 0.11 0.24 0.13 0.65

[0118] Table 13 Score of stinging sensation at each time point for Example 12 and Comparative Example 2

[0119] 0min 0.5min 2.5min 5min 10min sum Example 4 (left) 0 0.13 <![CDATA[0.24 * ]]> <![CDATA[0.28 ** ]]> <![CDATA[0.27 ** ]]> 0.92 Comparative Example 2 (right side) 0.07 0.28 0.41 0.71 0.68 2.15 Pain score 0.07 0.15 0.17 0.43 0.41 1.23

[0120] Table 14 Score of stinging sensation at each time point for Example 13 and Comparative Example 2

[0121] 0min 0.5min 2.5min 5min 10min sum Example 4 (left) 0.03 0.17 0.43 <![CDATA[0.56 * ]]> 0.66 1.85 Comparative Example 2 (right side) 0.09 0.32 0.47 0.75 0.72 2.35 Pain score 0.06 0.15 0.04 0.19 0.06 0.50

[0122] From the above series of tables, it can be seen that as the amount of GABA added increases, the tingling sensation increases; after adding NAG, this tingling sensation can be suppressed. Within a certain compounding ratio, as the amount of NAG added increases, the effect of suppressing the tingling sensation will increase, but NAG will produce burning and itching sensations, so too much NAG addition will have a negative effect on the tingling sensation.

[0123] In summary, the present invention can effectively alleviate the immediate discomfort, especially the tingling sensation, caused by the use of γ-aminobutyric acid by synergistically compounding γ-aminobutyric acid and N-acetyl glucosamine in a certain ratio.

[0124] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. The use of N-acetyl glucosamine in reducing the immediate discomfort of γ-aminobutyric acid; When the composition comprises γ-aminobutyric acid and N-acetyl glucosamine, the γ-aminobutyric acid is 0.3-5 parts by weight based on 100 parts by weight of the composition; The N-acetyl glucosamine is 0.1-10 parts.

2. The use according to claim 1, wherein The mass ratio of the γ-aminobutyric acid to the N-acetyl glucosamine is 1:0.1-4.

3. The use according to claim 1, wherein Based on 100 parts by weight of the composition, the amount of the gamma-aminobutyric acid is 2-4 parts.

4. The use according to claim 1, wherein Based on 100 parts by weight of the composition, the N-acetyl glucosamine is in an amount of 0.5-5 parts.

5. The use according to claim 1, wherein Based on 100 parts by weight of the composition, the N-acetyl glucosamine is 3-5 parts.

6. The use according to claim 1, wherein The mass ratio of the gamma-aminobutyric acid to the N-acetyl glucosamine is 1:1-2.

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