Waste Liquid Removal Mechanism and Nucleic Acid Extraction Device

Abstract

The present invention relates to a waste liquid removal mechanism and a nucleic acid extraction device. The waste liquid removal mechanism includes a moving mechanism and a liquid suction member. The liquid suction member is detachably connected to the moving mechanism. The moving mechanism is used to drive the liquid suction member to move to a liquid suction position. The liquid suction member includes a liquid suction material. When the moving mechanism drives the liquid suction member to move to the liquid suction position, at least a part of the liquid suction material is located in the waste liquid in the reaction tube. In this way, the moving mechanism drives the liquid suction member to move to the liquid suction position. At this time, the liquid suction member is inserted into the reaction tube, and at least a part of the liquid suction material is located in the waste liquid in the reaction tube, so as to adsorb the waste liquid by using the liquid suction material. Compared with the prior art in which a detachable pipette tip and a pipette pump are used for liquid suction and injection, the waste liquid removal mechanism in the present application uses the liquid suction material to adsorb the waste liquid to achieve the purpose of removing the waste liquid, does not impact the waste liquid, avoids the generation of aerosol, and there is no pollution risk caused by the accumulation of aerosol.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid extraction, and particularly to a waste liquid removing mechanism and a nucleic acid extraction device. Background Art

[0002] The nucleic acid extraction technology has experienced the development of technologies such as phenol extraction method, alkaline lysis method, CTAB (Cetyltrimethylammonium Bromide) extraction method, silica bead method, magnetic bead method, silica gel membrane method, etc. In recent years, with the development of instrument equipment, the automated nucleic acid extractor based on the magnetic bead method has been gradually widely used.

[0003] The automated nucleic acid extraction based on the magnetic bead method includes nucleic acid lysis and purification processes. After nucleic acid lysis and nucleic acid purification, nucleic acid is adsorbed by magnetic beads, and waste liquid is removed by a liquid transfer device. The existing liquid transfer device includes a detachable pipette tip and a liquid transfer pump. Aerosols are easily generated during liquid suction and injection, and the aerosols are easily accumulated at the liquid transfer channel during long-term use, posing a pollution risk. Summary of the Invention

[0004] Based on this, it is necessary to provide a waste liquid removing mechanism and a nucleic acid extraction device that can improve the above-mentioned defects in view of the problems that the nucleic acid extractor in the prior art is prone to generate aerosols during liquid suction and injection, and the aerosols are easily accumulated at the liquid transfer channel during long-term use, posing a pollution risk.

[0005] A waste liquid removing mechanism includes a moving mechanism and a liquid suction member. The liquid suction member is detachably connected to the moving mechanism. The moving mechanism is used to drive the liquid suction member to move to a liquid suction position. The liquid suction member includes a liquid suction material.

[0006] When the moving mechanism drives the liquid suction member to move to the liquid suction position, at least a part of the liquid suction material is located in the waste liquid in the reaction tube.

[0007] In one embodiment, the liquid suction member further includes a liquid suction tube. The liquid suction tube includes a tube body and a connecting portion fixedly connected to the tube body. The tube body has an inner cavity and a liquid inlet hole communicating with the inner cavity. The connecting portion is detachably connected to the moving mechanism. The liquid suction material is filled in the inner cavity and the liquid inlet hole of the liquid suction tube.

[0008] When the moving mechanism drives the liquid suction member to move to the liquid suction position, the liquid inlet hole is located in the waste liquid in the reaction tube. In this way, the inner cavity of the tube body is used to accommodate the liquid suction material and the waste liquid adsorbed by the liquid suction material, avoiding the dripping of the waste liquid adsorbed by the adsorbed material during the transfer process.

[0009] In one embodiment, the connecting portion is located at the top of the tube body, and the liquid inlet hole is located at the bottom of the tube body. In this way, the position of the liquid suction tube in the vertical direction can be adjusted by the moving mechanism to ensure that the liquid inlet hole is always below the liquid level of the waste liquid in the reaction tube during the liquid suction process.

[0010] In one embodiment, the liquid suction tube further includes a liquid inlet portion fixedly connected to the bottom of the tube body. The liquid inlet hole penetrates through the liquid inlet portion, and the outer diameter dimension of the liquid inlet portion is smaller than the outer diameter dimension of the tube body. In this way, driven by the moving mechanism, the liquid inlet portion first enters the reaction tube. Since the outer diameter dimension of the liquid inlet portion is smaller, the risk of collision between the liquid inlet portion and the reaction tube caused by the positioning error of the moving mechanism is reduced.

[0011] In one embodiment, the radial dimension of the liquid inlet portion gradually decreases from the end close to the tube body to the end far from the tube body. In this way, the risk of collision between the liquid inlet portion and the reaction tube caused by the positioning error of the moving mechanism is further reduced.

[0012] In one embodiment, the liquid inlet hole penetrates through the end of the liquid inlet portion close to the tube body and the end far from the tube body.

[0013] In one embodiment, the liquid inlet portion is further provided with a plurality of liquid inlet sub-holes. The plurality of liquid inlet sub-holes are arranged on the circumferential side wall of the liquid inlet portion and are all communicated with the liquid inlet hole. In this way, under the adsorption action of the liquid suction material, a part of the waste liquid in the reaction tube enters the liquid inlet hole from the bottom of the liquid inlet portion, and at the same time, a part of the waste liquid enters the liquid inlet hole from each liquid inlet sub-hole, and then enters the inner cavity of the tube body from the liquid inlet hole, thereby accelerating the adsorption speed of the waste liquid and improving the detection efficiency.

[0014] In one embodiment, the liquid suction material includes one or more of sponge, liquid suction foam material and liquid suction resin.

[0015] In one embodiment, the waste liquid removing mechanism includes a plurality of the liquid suction members, and each liquid suction member is detachably connected to the moving mechanism. In this way, the waste liquid in a plurality of reaction tubes can be transferred simultaneously, further improving the detection efficiency.

[0016] In one embodiment, the waste liquid removing mechanism further includes a liquid level tracker installed on the liquid suction member or the moving mechanism. The liquid level tracker is communicatively connected to the moving mechanism;

[0017] The liquid level tracker is used to detect the liquid level position in the reaction tube, and control the moving mechanism to drive the liquid suction member to move into the reaction tube according to the detected liquid level position in the reaction tube, so that at least part of the liquid suction material is kept below the liquid level in the reaction tube. In this way, by detecting the liquid level position in real time with the liquid level tracker and controlling the moving mechanism to drive the liquid suction member to move into the reaction tube, the speed at which the pressing surface descends is approximately equal to the speed at which the liquid suction member moves into the reaction tube, thereby ensuring that at least part of the liquid suction material is always kept below the liquid level in the reaction tube.

[0018] A nucleic acid extraction device includes the waste liquid removing mechanism described in any of the above embodiments.

[0019] In the actual use process of the above waste liquid removing mechanism and nucleic acid extraction device, the moving mechanism drives the liquid suction member to move to the liquid suction position. At this time, the liquid suction member is inserted into the reaction tube, and at least part of the liquid suction material is located in the waste liquid in the reaction tube, so as to adsorb the waste liquid by using the liquid suction material. After the liquid suction material finishes adsorbing, the moving mechanism drives the liquid suction member to withdraw from the reaction tube, then controls the separation of the moving mechanism and the liquid suction member, and replaces it with a new liquid suction member to prepare for the next waste liquid removal.

[0020] In this way, compared with the prior art in which a detachable gun head and a pipette pump are used for liquid suction and injection, the waste liquid removing mechanism in the present application uses the liquid suction material to adsorb the waste liquid to achieve the purpose of removing the waste liquid, does not impact the waste liquid, avoids the generation of aerosol, and there is no pollution risk caused by the accumulation of aerosol. Description of the Drawings

[0021] Figure 1 It is a cross-sectional view of the liquid suction member of the waste liquid removing mechanism in an embodiment of the present invention. Detailed Embodiments

[0022] In order to make the above objects, features and advantages of the present invention more obvious and understandable, the following detailed description of the specific embodiments of the present invention will be made in conjunction with the drawings. Many specific details are set forth in the following description in order to fully understand the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0023] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0024] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present invention, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically and clearly defined.

[0025] In the present invention, unless otherwise clearly specified and defined, terms such as "mounted", "connected", "coupled", "fixed", etc. shall be construed in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements, unless otherwise clearly defined. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0026] In the present invention, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0027] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or there may be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation.

[0028] Please refer to Figure 1 , an embodiment of the present invention provides a waste liquid removal mechanism for removing the waste liquid 21 in the reaction tube 20 after the magnetic beads 22 in the reaction tube 20 are adsorbed and fixed.

[0029] The waste liquid removal mechanism includes a moving mechanism (not shown in the figure) and a liquid absorbing member 10. The liquid absorbing member 10 is detachably connected to the moving mechanism, and the moving mechanism is used to drive the liquid absorbing member 10 to move to the liquid absorbing position. The liquid absorbing member 10 includes a liquid absorbing material 12 for absorbing the waste liquid 21. When the moving mechanism drives the liquid absorbing member 10 to move to the liquid absorbing position, at least a part of the liquid absorbing material 12 is located in the waste liquid 21 in the reaction tube 20, so that the liquid absorbing material 12 absorbs the waste liquid 21.

[0030] In the actual use process of the above waste liquid removal mechanism, the moving mechanism drives the liquid absorbing member 10 to move to the liquid absorbing position. At this time, the liquid absorbing member 10 is inserted into the reaction tube 20, and at least a part of the liquid absorbing material 12 is located in the waste liquid 21 of the reaction tube 20, so as to adsorb the waste liquid 21 by using the liquid absorbing material 12. After the liquid absorbing material 12 finishes adsorption, the moving mechanism drives the liquid absorbing member 10 to withdraw from the reaction tube 20, then controls the separation of the moving mechanism and the liquid absorbing member 10, and replaces it with a new liquid absorbing member 10 to prepare for the next waste liquid removal.

[0031] In this way, compared with the method of using a detachable pipette tip and a pipette pump for liquid suction and injection in the prior art, the waste liquid removal mechanism in the present application uses the liquid absorbing material 12 to adsorb the waste liquid 21 to achieve the purpose of removing the waste liquid 21, does not generate an impact on the waste liquid 21, avoids the generation of aerosols, and there is no pollution risk caused by the accumulation of aerosols.

[0032] It should be noted that the method of using a detachable pipette tip and a pipette pump for liquid suction and injection in the prior art has relatively high requirements for the position accuracy of instrument calibration, a large consumption of consumables, a high cost, and a complex structure. However, the waste liquid removal mechanism in the present application uses the liquid absorbing material 12 to adsorb the waste liquid 21, has a simple structure, greatly reduces the consumption of consumables, reduces the cost, and only needs to insert and withdraw the liquid absorbing member 10 into and out of the reaction tube 20, with relatively low requirements for position accuracy.

[0033] It should also be noted that in the present application, the waste liquid removal mechanism uses the liquid absorption material 12 to adsorb the waste liquid 21. The absorption speed of the absorption material is relatively fast, thereby greatly reducing the time required to remove the waste liquid 21 and improving the detection efficiency.

[0034] Optionally, the liquid absorption material 12 includes one or more of sponge, liquid absorption foam material, and liquid absorption resin. Of course, in some other embodiments, other materials capable of adsorbing the waste liquid 21 may also be used, which is not limited herein.

[0035] In an embodiment of the present invention, the liquid absorption member 10 further includes a liquid absorption tube 11. The liquid absorption tube 11 includes a tube body 111 and a connection portion 112 fixedly connected to the tube body 111. The tube body 111 has an inner cavity 1111 and a liquid inlet hole 1131 communicating with the inner cavity 1111. The connection portion 112 is detachably connected to the moving mechanism, and the liquid absorption material 12 is filled in the inner cavity 1111 and the liquid inlet hole 1131 of the liquid absorption tube 11.

[0036] When the moving mechanism drives the liquid absorption tube 11 to move to the liquid absorption position, the liquid inlet hole 1131 is located in the waste liquid 21 in the reaction tube 20, so that the waste liquid 21 flows from the liquid inlet hole 1131 into the inner cavity 1111 of the tube body 111 under the action of the adsorption force of the liquid absorption material 12. In this way, the inner cavity 1111 of the tube body 111 is used to accommodate the liquid absorption material 12 and the waste liquid 21 adsorbed by the liquid absorption material 12, avoiding the dripping of the waste liquid 21 adsorbed by the adsorption material during the transfer process.

[0037] Specifically, in the embodiment, the connection portion 112 is located at the top of the tube body 111, and the liquid inlet hole 1131 is located at the bottom of the tube body 111. The connection portion 112 at the top of the liquid absorption tube 11 is connected to the moving mechanism, and the liquid inlet hole 1131 at the bottom of the liquid absorption tube 11 is used for the waste liquid 21 to enter the inner cavity 1111 of the tube body 111. In this way, the position of the liquid absorption tube 11 in the vertical direction can be adjusted by the moving mechanism to ensure that the liquid inlet hole 1131 is always below the liquid level of the waste liquid 21 in the reaction tube 20 during the liquid absorption process.

[0038] Specifically, in the embodiment, the liquid absorption tube 11 further includes a liquid inlet portion 113 fixedly connected to the bottom of the tube body 111, and the liquid inlet hole 1131 penetrates through the liquid inlet portion 113. The outer diameter of the liquid inlet portion 113 is smaller than the outer diameter of the tube body 111. In this way, under the drive of the moving mechanism, the liquid inlet portion 113 first enters the reaction tube 20. Since the outer diameter of the liquid inlet portion 113 is relatively small, the risk of collision between the liquid inlet portion 113 and the reaction tube 20 caused by the positioning error of the moving mechanism is reduced. Preferably, the radial dimension of the liquid inlet portion 113 gradually decreases from the end close to the tube body 111 to the end far from the tube body 111, further reducing the risk of collision between the liquid inlet portion 113 and the reaction tube 20 caused by the positioning error of the moving mechanism.

[0039] Further, the liquid inlet hole 1131 penetrates through one end of the liquid inlet part 113 close to the tube body 111 and the other end far from the tube body 111. Thus, under the adsorption of the liquid absorption material 12, the waste liquid 21 in the reaction tube 20 enters the liquid inlet hole 1131 from the bottom of the liquid inlet part 113, and then enters the inner cavity 1111 of the tube body 111 from the liquid inlet hole 1131.

[0040] Further, the liquid inlet part 113 is further provided with a plurality of liquid inlet sub-holes (not shown in the figure). The plurality of liquid inlet sub-holes are arranged on the circumferential side wall of the liquid inlet part 113 and are all communicated with the liquid inlet hole 1131. In this way, under the adsorption of the liquid absorption material 12, a part of the waste liquid 21 in the reaction tube 20 enters the liquid inlet hole 1131 from the bottom of the liquid inlet part 113, and at the same time, a part of the waste liquid 21 enters the liquid inlet hole 1131 from each liquid inlet sub-hole, and then enters the inner cavity 1111 of the tube body 111 from the liquid inlet hole 1131, thereby accelerating the adsorption speed of the waste liquid 21 and improving the detection efficiency.

[0041] In the embodiment of the present invention, the waste liquid mechanism includes a plurality of liquid absorption members 10. Each liquid absorption member 10 is detachably connected to the moving mechanism, so as to transfer the waste liquid in a plurality of reaction tubes 20 at the same time, further improving the detection efficiency.

[0042] In the embodiment of the present invention, the moving mechanism is further used to drive the liquid absorption member 10 to move to the separation position. When the moving mechanism drives the liquid absorption member 10 to move to the separation position, the liquid absorption member 10 exits outside the reaction tube 20, and the connecting part 112 is separated from the moving mechanism, thereby realizing the transfer of the liquid absorption member 10 adsorbed with the waste liquid 21.

[0043] Further, the separation position is located in the waste collection area. Thus, when the liquid absorption member 10 moves to the separation position, the liquid absorption member 10 is separated from the moving mechanism and falls into the waste collection area, so as to collect and manage the waste.

[0044] It should be noted that the connecting part 112 and the moving mechanism can be detachably connected by means of clamping, snap connection, tightening, etc., which is not limited herein.

[0045] Optionally, the moving mechanism can be a robotic arm. Of course, in other embodiments, the moving mechanism can also adopt other structures, as long as it can realize driving the liquid absorption member 10 to transfer, which is not limited herein.

[0046] During the liquid absorption process of the liquid absorption material 12, since the waste liquid 21 in the reaction tube 20 gradually decreases, the liquid level in the reaction tube 20 gradually drops. In order to enable the liquid absorption material 12 to continuously adsorb the waste liquid 21 in the reaction tube 20, it is necessary to ensure that at least part of the liquid absorption material 12 is always below the liquid level.

[0047] In an embodiment of the present invention, in addition to the waste liquid mechanism, a liquid level tracker (not shown in the figure) is further included, which is installed on the liquid suction member 10 or the moving mechanism, and the liquid level tracker is communicatively connected to the moving mechanism. The liquid level tracker is used to detect the position of the liquid level in the reaction tube 20, and control the moving mechanism to drive the liquid suction member 10 to move into the reaction tube 20 according to the detected liquid level position in the reaction tube 20, so that at least part of the liquid suction material 12 is always located below the liquid level in the reaction tube 20, so as to facilitate the liquid suction material 12 to continuously suck the waste liquid 21 in the reaction tube 20.

[0048] It can be understood that by detecting the liquid level position in real time with the liquid level tracker and controlling the moving mechanism to drive the liquid suction member 10 to move into the reaction tube 20, the speed at which the liquid surface drops is approximately equal to the speed at which the liquid suction member 10 moves into the reaction tube 20, so as to ensure that at least part of the liquid suction material 12 is always located below the liquid level in the reaction tube 20.

[0049] In one embodiment, the liquid level tracker has a detection circuit communicatively connected to the moving mechanism and two conductive members electrically connected to the detection circuit. When the two conductive members are located below the liquid level, the two conductive members are conducted through the waste liquid 21. When the liquid level drops and the two conductive members are located above the liquid level, the two conductive members are disconnected from each other. In this way, when the detection circuit detects that the two conductive members are disconnected from each other, it controls the moving mechanism to drive the liquid suction member 10 to move into the reaction tube 20 until the detection circuit detects that the two conductive members are electrically conducted, so as to realize liquid level tracking. It can be understood that when the two conductive members are located below the liquid level, the liquid suction material 12 is also located below the liquid level.

[0050] In another embodiment, the liquid level tracker can be a ranging camera, which is used to detect the liquid level position in the reaction tube 20 in real time, and control the moving mechanism to drive the liquid suction member 10 to move into the reaction tube 20 according to the detected liquid level position, so as to ensure that at least part of the liquid suction material 12 is located below the liquid level in the reaction tube 20 and continuously suck the liquid.

[0051] In yet another embodiment, the liquid level tracker calculates the dropping speed of the liquid level in the reaction tube 20 based on the liquid addition amount in the reaction tube 20, the cross-sectional area of the reaction tube 20, and the liquid suction speed of the liquid suction material 12, so as to control the moving speed of the liquid suction member 10 moving into the reaction tube 20 through the moving mechanism, so that the moving speed of the liquid suction member 10 moving into the reaction tube 20 is approximately equal to the dropping speed of the liquid level in the reaction tube 20. It should be noted that since the liquid suction speed of the liquid suction material 12 is not a fixed value but variable during the liquid suction process, the dropping speed of the liquid level in the reaction tube 20 is also variable, and thus the moving speed of the moving mechanism driving the liquid suction member 10 to move into the reaction tube 20 also needs to change accordingly.

[0052] Based on the above waste liquid removing mechanism, the present invention further provides a nucleic acid extraction device, which includes the waste liquid removing mechanism described in any of the above embodiments.

[0053] Specifically, the nucleic acid extraction device further includes a deep well plate, a vibration mechanism, and a magnetic attraction mechanism. The deep well plate is used to load reaction tubes 20, and a solution and magnetic beads 22 are added into the reaction tubes 20. The deep well plate is assembled on the vibration mechanism and is used to vibrate the deep well plate so as to mix the solution in the reaction tubes 20 and react with the magnetic beads 22. The magnetic attraction mechanism is used to adsorb and fix the magnetic beads 22 in the reaction tubes 20 after the reaction is completed. After the magnetic beads 22 in the reaction tubes 20 are adsorbed and fixed, the moving mechanism drives the liquid suction member 10 to insert into the reaction tubes 20 (i.e., the liquid suction position), so as to adsorb the solution (i.e., waste liquid 21) in the reaction tubes 20 by using the liquid suction material 12. After the adsorption is completed, the moving mechanism drives the liquid suction member 10 to withdraw from the reaction tubes 20 and transfer to the separation position, and then controls the separation of the moving mechanism from the liquid suction member 10, so that the liquid suction member 10 drops into the waste collection area.

[0054] It should be noted that the specific structures of the deep well plate, the vibration mechanism, and the magnetic attraction mechanism can adopt relatively mature existing technologies and will not be elaborated here.

[0055] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0056] The above embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be understood as a limitation on the scope of the invention patent. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent should be subject to the appended claims.

Claims

1. A waste liquid removing mechanism, characterized in that, it includes a moving mechanism and a liquid absorbing member (10). The liquid absorbing member (10) is detachably connected to the moving mechanism. The moving mechanism is used to drive the liquid absorbing member (10) to move. The liquid absorbing member (10) includes a liquid absorbing material (12); The liquid absorbing member (10) further includes a liquid absorbing tube (11). The liquid absorbing tube (11) includes a tube body (111) and a connecting portion (112) fixedly connected to the tube body (111). The tube body (111) has an inner cavity (1111) and a liquid inlet hole (1131) communicating with the inner cavity (1111). The connecting portion (112) is detachably connected to the moving mechanism. The liquid absorbing material (12) is filled in the inner cavity (1111) of the liquid absorbing tube (11) and the liquid inlet hole (1131); When the moving mechanism drives the liquid absorbing member (10) to move to the liquid absorbing position, the liquid inlet hole (1131) is located in the waste liquid (21) in the reaction tube (20); The liquid absorbing tube (11) further includes a liquid inlet portion (113) fixedly connected to the bottom of the tube body (111). The liquid inlet hole (1131) penetrates through the liquid inlet portion (113). The outer diameter dimension of the liquid inlet portion (113) is smaller than the outer diameter dimension of the tube body (111). The liquid inlet hole (1131) penetrates through one end of the liquid inlet portion (113) close to the tube body (111) and one end far from the tube body (111).

2. The waste liquid removing mechanism according to claim 1, characterized in that, the connecting portion (112) is located at the top of the tube body (111), and the liquid inlet hole (1131) is located at the bottom of the tube body (111).

3. The waste liquid removing mechanism according to claim 1, characterized in that, the radial dimension of the liquid inlet portion (113) gradually decreases from the end close to the tube body (111) to the end far from the tube body (111).

4. The waste liquid removing mechanism according to claim 1, characterized in that, the liquid inlet portion (113) is further provided with a plurality of liquid inlet sub-holes. The plurality of liquid inlet sub-holes are arranged on the circumferential side wall of the liquid inlet portion (113) and are all communicated with the liquid inlet hole (1131).

5. The waste liquid removing mechanism according to any one of claims 1 to 4, characterized in that, the liquid absorbing material (12) includes one or more of sponge, liquid absorbing foam material and liquid absorbing resin.

6. The waste liquid removing mechanism according to any one of claims 1 to 4, characterized in that, the waste liquid removing mechanism includes a plurality of the liquid absorbing members (10), and each liquid absorbing member (10) is detachably connected to the moving mechanism.

7. The waste liquid removing mechanism according to any one of claims 1 to 4, characterized in that, the waste liquid removing mechanism further includes a liquid level tracker installed on the liquid absorbing member (10) or the moving mechanism. The liquid level tracker is communicatively connected to the moving mechanism; The liquid level tracker is used to detect the liquid level position in the reaction tube (20), and control the moving mechanism to drive the liquid absorbing member (10) to move into the reaction tube (20) according to the detected liquid level position in the reaction tube (20), so that at least part of the liquid absorbing material (12) is kept below the liquid level in the reaction tube (20).

8. A nucleic acid extraction device, characterized in that it includes a waste liquid removing mechanism as described in any one of claims 1 to 7.

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