A method for breeding hybrid rice that takes both nutrition and ornamental value into consideration

Abstract

The invention discloses a method for breeding hybrid rice that takes both nutrition and ornamental into consideration, the method comprising: using colored rice A +彩色叶 As the male parent, the two-line hybrid rice sterile line S or restorer line R is the female parent, the hybrid progeny is backcrossed with the female parent, and the BC3F2 generation seeds are harvested; the purple seed coat sterile line S1 is selected for direct seeding in the BC3F2 generation +彩色叶+紫色种皮 Seeds; when mature, select the color leaf restorer line R1 +彩色叶 Seeds; Select the sterile line S2 +彩色叶+紫色种皮 and the restorer line R2 with normal fertility +彩色叶 ; Paired test to obtain hybrid F1; then select the sterile line S5 with good ornamental value +彩色叶+紫色种皮 ; Obtaining hybrid rice with both nutritional and ornamental value +彩色叶+紫色种皮 The present invention combines colored rice with purple hybrid rice through backcrossing and testing and breeding to produce a hybrid rice that takes both nutrition and ornamental into consideration. It is not only a new variety of purple hybrid rice with nutrition, but also suitable for the development of creative agriculture, realizing the combination of agriculture and tourism.

Description

Technical Field

[0001] The invention relates to the technical field of rice breeding, and in particular to a breeding method for hybrid rice that takes both nutrition and ornamental into consideration. Background Art

[0002] Industrial integration is an important way to promote high-quality agricultural development, among which the combination of agriculture and tourism has been recognized by the public. To this end, rice field ideas with colored rice as the starting point have been used in our motherland one after another. Taking into account both nutrition and appreciation is the core element of future Rice Plus, and it is also the key to promoting agricultural development through science and technology, promoting industrial prosperity and helping rural revitalization.

[0003] Colored rice is made from brown rice, which contains seed coat, rice germ and endosperm. Therefore, its content of protein, fat, amino acid, vitamin and mineral trace elements is significantly higher than that of white rice (polished rice) consumed daily. It is also rich in natural pigments and a variety of physiologically active substances, which is very consistent with my country's traditional food culture of "food and medicine". In the current context of deteriorating ecological environment, significantly different lifestyles and significantly faster pace of life, the dietary structure has undergone tremendous changes. Various chronic diseases such as "three highs", atherosclerosis, coronary heart disease and cancer have become more frequent year by year, bringing pain to society and families. Eating well, eating enough and eating healthy has become a fashionable health pursuit. Colored rice has become a healthy choice for many consumers because it has more complete nutrition and contains physiologically active substances that can prevent the occurrence of various chronic diseases.

[0004] To this end, the present invention proposes a method for breeding hybrid rice that takes both nutrition and ornamental properties into consideration, aiming to provide a new variety of colorful purple hybrid rice that is beautiful, delicious and more nutritious, and can be better used in creative agriculture to promote rural revitalization. Summary of the invention

[0005] The present invention provides a method for breeding hybrid rice that takes both nutrition and ornamental into consideration. The breeding method obtains colorful purple rice hybrid rice with strong ornamental value, good taste and greater nutrition, which is suitable for the development of creative agriculture and helps the rural revitalization strategy.

[0006] The specific technical solutions are as follows:

[0007] A method for breeding hybrid rice that takes both nutrition and ornamental value into consideration, comprising the following steps:

[0008] (1) Colored Rice A +彩色叶 As the male parent, the two-line hybrid rice sterile line S or restorer line R of purple rice is the female parent, and hybridization is performed. The obtained progeny is backcrossed with the female parent three times to obtain the BC3F1 generation. After the BC3F1 generation is self-pollinated, the BC3F2 generation seeds are harvested;

[0009] (2) Uniformly sparsely seed the BC3F2 generation in the nursery field, select the plants with colored leaves when the seedlings have 4-5 leaves, and transplant them into the field. When they bloom, select the male sterile plants with pollen abortion, cut the male sterile plants and regenerate them, and select the sterile plants that have changed from fertility to fertility, and select the purple seed coat sterile line S1 +彩色叶+紫色种皮 The seeds of the plant; when mature, select the excellent color leaf restorer line R1 +彩色叶 seeds of plants;

[0010] (3) The purple seed sterile line S1 obtained in step (2) +彩色叶+紫色种皮 The seeds of the plants were planted for multiple generations, and the obtained seeds were planted in stages. The sterile line S of the purple rice two-line hybrid rice was used as the control variety to observe the sterile line S1 +彩色叶+紫色种皮 The sterility of S2 was selected to be completely sterile. +彩色叶+紫色种皮 , using the restorer line R as control, and observing the restorer line R1 +彩色叶 , select the restorer line R2 with normal fertility +彩色叶 ;

[0011] (4) The sterile line S2 obtained in step (3) +彩色叶+紫色种皮 and Restoration R2 +彩色叶 , pair-matching, obtain hybrid F1, observe and identify the leaf and seed coat color and heterosis of hybrid F1, select hybrid F1 with strong heterosis +彩色叶+紫色种皮 and the corresponding sterile line S3 +彩色叶+紫色种皮 With the recovery system R3 +彩色叶 ;

[0012] (5) For the sterile line S3 +彩色叶+紫色种皮 Conduct indoor and outdoor fertility identification and select the sterile line S4 that meets the sterile line characteristics +彩色叶+紫色种皮 , planted with local production and promotion varieties, and select the sterile line S5 with good ornamental value +彩色叶+紫色种皮 and with the recovery system R3 +彩色叶 Retest and identify hybrid F1 again +彩色叶+紫色种皮 ;

[0013] (6) Construction of the sterile line S5 for both nutritional and ornamental purposes +彩色叶+紫色种皮 , Restoration R3 +彩色叶 New hybrid rice varieties prepared by the method +彩色叶+紫色种皮 Production system, finally choose hybrid rice with both nutritional and ornamental value +彩色叶+紫色种皮 .

[0014] Furthermore, the colored rice A +彩色叶 Any of a variety of rice with yellow or silvery leaves that is recessive to green leaves.

[0015] Furthermore, the colored rice A +彩色叶 It is Yin Caihe from Zhejiang University.

[0016] ZJU Yincaihe is a special colored rice variety suitable for the development of creative agriculture, which was bred by Zhejiang University. The variety was approved by the Zhejiang Provincial Crop Variety Approval Committee in 2019 (Zhejiang Approval Rice 20190011), and applied for new variety protection by the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs (application number: 2019100128).

[0017] Furthermore, the two-line sterile line S and the restorer line R are a purple rice two-line hybrid rice combination.

[0018] Furthermore, the sterile line S of the purple rice two-line hybrid rice is Zheda Zi 1S; and the restorer line R is Zhefu 01.

[0019] ZJUZI1S is a thermosensitive sterile line of purple rice bred by Zhejiang University. The variety has been approved by the Zhejiang Provincial Crop Variety Approval Committee in 2021 (Zheshen Rice [Sterile Line] 2021008), and has applied for new variety protection from the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs (application number: 20201005465).

[0020] Zhejiang Fu 01 is a restoration line bred by Zhejiang University. It was formulated with Zhejiang University Purple 1S to produce a new purple hybrid rice combination, Zhejiang University Purple Liangyou 1 (Zheshen Rice 2021036). It was approved by the Zhejiang Provincial Crop Variety Approval Committee in 2021 and applied to the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs for new variety protection (application number: 20201006977).

[0021] Furthermore, in step (3), the planting generation number is ≥ 6.

[0022] In step (4), the high-generation sterile line S +彩色叶+紫色种皮 and recovery system R +彩色叶 When testing in pairs, select S +彩色叶 With R +彩色叶 Different colored leaves, select the hybrid F1 with strong advantages +彩色叶+紫色种皮 With colored rice A +彩色叶 The viewing experience is different.

[0023] Furthermore, in step (4), the evaluation indicators for strong hybrid vigor include: seed coat color, yield, rice quality and disease resistance; among which, the seed coat color is purple, the yield is ≥600kg / mu, the rice quality is the rice edible quality reaching the ministerial level three or above standard, and the disease resistance is moderate resistance to rice blast.

[0024] Furthermore, in step (5), the characteristics of the sterile line are based on the standard NY / T 215-2006 issued by the Ministry of Agriculture.

[0025] Furthermore, in step (5), the criteria for judging the ornamental value are that the growth period, plant height and lodging resistance are all higher than those of the parent.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] (1) The present invention combines colored rice with approved purple rice hybrid rice and then backcrosses it to produce a hybrid rice that takes both nutrition and ornamental into consideration. This is not only a new variety of purple rice hybrid rice, but also suitable for the development of creative agriculture, thus realizing the combination of agriculture and tourism.

[0028] (2) The present invention uses colored rice A +彩色叶 , as a donor of ornamental traits, and hybridized and backcrossed with sterile lines and restorer lines. Considering the fast breeding cycle and avoiding homogeneity affecting heterosis, the high-generation sterile line S was backcrossed only three times. +彩色叶+紫色种皮 and recovery system R +彩色叶 When testing in pairs, select S +彩色叶 With R +彩色叶 The hybrid F1 is selected for leaves with different colors +彩色叶+紫色种皮 With colored rice A +彩色叶 The ornamental value is different, which not only better preserves the hybrid advantage, but also adds the future nutritional and ornamental hybrid rice +彩色叶+紫色种皮 With colored rice A +彩色叶 The space advantages of matching applications.

[0029] (3) The hybrid rice obtained by the present invention has a significantly higher lysine content in its grains, better nutritional and taste quality, better nutritional and ornamental properties, and is good-looking, delicious and nutritious, which is conducive to promoting industrial prosperity. DETAILED DESCRIPTION

[0030] The present invention will be further described below in conjunction with specific embodiments. The following are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto.

[0031] The breeding method provided in the following examples is a breeding method for hybrid rice that takes both nutritional and ornamental considerations into account based on Zheda Yincaihe and Zheda Ziliangyou 1 (a two-line sterile line Zheda Zi 1S and a restorer line Zhefu 01).

[0032] Among them, Zheda Yincaihe is a special colored rice variety suitable for the development of creative agriculture, which was bred by Zhejiang University in cooperation. This variety has been approved by the Zhejiang Provincial Crop Variety Approval Committee in 2019 (Zheshen Rice 20190011), and has applied for new variety protection from the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs (application number: 2019100128), and can be purchased from the Institute of Nuclear Agricultural Sciences of Zhejiang University.

[0033] Zhejiang University Ziliangyou No. 1 is a new combination of purple rice hybrid rice bred by Zhejiang University using Zhejiang University Zi 1S and Zhejiang Fu 01. It has passed the approval of the Zhejiang Provincial Crop Variety Approval Committee in 2021 (Zheshen Rice 2021036), and applied for new variety protection from the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs (application number: 20201006977). It can be purchased from the Institute of Nuclear Agricultural Sciences of Zhejiang University.

[0034] ZJUZI1S is a thermosensitive sterile line of purple rice bred by Zhejiang University. This variety has been approved by the Zhejiang Provincial Crop Variety Approval Committee in 2021 (Zheshen Rice [sterile line] 2021008), and has applied for new variety protection from the Plant Variety Protection Office of the Ministry of Agriculture and Rural Affairs (application number: 20201005465). It can be purchased from the Institute of Nuclear Agricultural Sciences of Zhejiang University.

[0035] Xiushui 134 is a single-season conventional late japonica rice bred by Jiaxing Academy of Agricultural Sciences and other institutions. It was awarded second grade by the Ministry of Rice Quality. It passed the Zhejiang Provincial Appraisal in 2010. The approval certificate number is Zhejiang Appraisal Rice 2010003. It is the main cultivated variety currently produced and promoted in Zhejiang Province and the control variety for regional trials. It can be purchased from Jiaxing Academy of Agricultural Sciences or seed markets.

[0036] Example 1 Study on Genetic Expression of Silvery-white Leaves and Purple Seed Coats

[0037] The ZJU Yincaihe was hybridized with the ZJU Zi 1S and the restorer Zhefu 01. The results in Table 1 show that the silvery-white leaves and normal green color are recessive, while the purple seed coat is explicit. This means that the restoration line of the sterile rice should be improved simultaneously based on the ZJU Ziliangyou 1 in order to improve the ornamental traits. As for the purple seed coat, the nutritional quality of hybrid rice can be obtained by changing the sterile line.

[0038] Table 1 Genetic expression analysis of silvery white leaves and purple seed coat

[0039]

[0040] Example 2

[0041] A method for breeding hybrid rice that takes both nutrition and ornamental value into consideration, the specific steps are as follows:

[0042] (1) Colored Rice A +彩色叶 The Yincaihe of Zhejiang University was used as the male parent, and the two-line hybrid rice sterile line S (Zhejiang University Zi1S) was used as the female parent. The progeny obtained were backcrossed with the female parent three times to obtain the BC3F1 generation. After the BC3F1 generation was self-pollinated, the seeds of the BC3F2 generation were harvested, including more than 3,000 seeds of the improved sterile line and more than 1,000 seeds of the improved restorer line.

[0043] (2) Uniformly sparsely seeded BC3F2 in the nursery field, 159 silvery white plants were selected when the seedlings had 4-5 leaves and transplanted into the field. When they were flowering, 47 male sterile plants with pollen abortion were selected and regenerated. The male sterile plants were cut and regenerated, and the sterile plants with fertility converted to fertile were selected. The purple seed coat sterile line S1 was selected. +彩色叶+紫色种皮 Seeds of 36 plants; improved restorer line R, selected restorer line R1 with excellent color leaves when mature +彩色叶 Seeds from 86 plants.

[0044] (3) The purple seed sterile line S1 obtained in step (2) +彩色叶+紫色种皮 The seeds of the plants were planted for multiple generations, and the obtained seeds were planted in stages. The sterile line S of the purple rice two-line hybrid rice was used as the control variety to observe the sterile line S1 +彩色叶+紫色种皮 The sterility of S2 was selected to be completely sterile. +彩色叶+紫色种皮 19, with the restorer line R as the control, the restorer line R1 was observed +彩色叶 , select the excellent restorer line R2 with normal fertility +彩色叶 40;

[0045] (4) The sterile line S2 obtained in step (3) +彩色叶+紫色种皮 and Restoration R2 +彩色叶 , pair-matching, obtain hybrid F1, observe and identify the leaf and seed coat color and hybrid vigor of hybrid F1, select hybrid F1 with strong vigor +彩色叶+紫色种皮 8 and the corresponding sterile line S3 +彩色叶+紫色种皮 8 and Restoration R3 +彩色叶 8;

[0046] (5) For the sterile line S3 +彩色叶+紫色种皮 Conduct indoor and outdoor fertility identification, and select the sterile line S4 that meets the sterile line characteristics according to the standard NY / T 215-2006 issued by the Ministry of Agriculture. +彩色叶+紫色种皮 6, planted with local production and promotion varieties such as Xiushui 134 for creative agriculture, and select S5 for good ornamental value +彩色叶+紫色种皮 3, and with the corresponding recovery system R3 +彩色叶 Retest and identify hybrid F1 again +彩色叶+紫色种皮 ;

[0047] (6) Construction of the sterile line S5 for both nutritional and ornamental purposes +彩色叶+紫色种皮 , Restoration R3 +彩色叶 New hybrid rice varieties prepared by the method +彩色叶+紫色种皮 Production system, finally choose hybrid rice with both nutritional and ornamental value +彩色叶+紫色种皮 1, initially named Yin Zi Liang You No. 1.

[0048] The comparison of seed coat color, blackness, black rice pigment, lysine, and nutritional and taste quality of each variety is shown in Table 2.

[0049] Table 2 Comparison of index traits of hybrid rice based on Zhejiang University Yincaihe and Zhejiang University Ziliangyou No. 1 with both nutritional and ornamental characteristics

[0050]

[0051] The hybrid rice "Yin Zi Liang You No. 1" with both nutritional and ornamental characteristics was bred through identification of variety specificity, consistency and stability. It has striped silver-white leaves, purple seed coat, blackness of 90%, black rice pigment content E 2.33, lysine content of 0.35g / 100g, yield ≥610kg / mu, rice quality reaches the second level issued by the ministry, and is moderately resistant to rice blast. Compared with the parents Zheda Yin Cai He, Zheda Zi 1S, Zhe Fu 01 and the target hybrid rice combination Yin Zi Liang You No. 1, the new hybrid rice variety bred by the present invention, while taking into account the advantages of both parents, presents the ornamental trait of striped silver-white, which is convenient for future matching with Zheda Yin Cai He. It may be related to the striped silver-white trait, and the lysine content of its grain is significantly higher.

[0052] Lysine is one of the essential amino acids for the human body, which can promote human development, enhance immune function, and improve the function of central nervous system tissue. The lysine content in cereal foods including rice is very low, and it is easily destroyed and lacking during processing, so it is called the first limiting amino acid. Therefore, the "Silver Purple Two Excellent No. 1" bred by the present invention has better nutritional and taste quality, and better nutritional and ornamental properties.

Claims

1. A method for breeding hybrid rice that takes both nutritional and ornamental properties into consideration, characterized in that: The following steps are involved: (1) Colored Rice A +彩色叶 As the male parent, the two-line hybrid rice sterile line S and the restorer line R of purple rice were used as the female parents, and the hybridization was carried out. The obtained progeny were backcrossed with the female parent three times to obtain the BC3F1 generation. After the BC3F1 generation was self-pollinated, the BC3F2 generation seeds were harvested; The colored rice A +彩色叶 For Zhejiang University Yin Caihe; The two-line sterile line S and the restorer line R are a purple rice two-line hybrid rice combination; the sterile line S of the purple rice two-line hybrid rice is Zheda Zi 1S, and the restorer line R is Zhefu 01; (2) Uniformly sparsely seed the BC3F2 generation in the nursery field, select the plants with colored leaves when the seedlings have 4-5 leaves, and transplant them into the field; select the male sterile plants with pollen abortion when they bloom, cut the stumps of the male sterile plants and regenerate them, and select the sterile plants that have changed from fertility to fertility, and select the sterile line plants with purple seed coat S1 +彩色叶+紫色种皮 The seeds of the plant; when mature, select the excellent color leaf restorer line R1 +彩色叶 seeds of plants; (3) The purple seed sterile line S1 obtained in step (2) +彩色叶+紫色种皮 The seeds of the plants were planted for multiple generations, and the obtained seeds were planted in stages. The sterile line S of the purple rice two-line hybrid rice was used as the control variety to observe the sterile line S1 +彩色叶+紫色种皮 The sterility of S2 was selected to be completely sterile. +彩色叶+紫色种皮 , using the restorer line R as control, and observing the restorer line R1 +彩色叶 , select the restorer line R2 with normal fertility +彩色叶 ; (4) The sterile line S2 obtained in step (3) +彩色叶+紫色种皮 and Restoration R2 +彩色叶 , pair-matching, obtain hybrid F1, observe and identify the leaf and seed coat color and heterosis of hybrid F1, select hybrid F1 with strong heterosis +彩色叶+紫色种皮 and the corresponding sterile line S3 +彩色叶+紫色种皮 With the recovery system R3 +彩色叶 ; (5) For the sterile line S3 +彩色叶+紫色种皮 Conduct indoor and outdoor fertility identification and select the sterile line S4 that meets the sterile line characteristics +彩色叶+紫色种皮 , planted with local production and promotion varieties, and select the sterile line S5 with good ornamental value +彩色叶+紫色种皮 and with the recovery system R3 +彩色叶 Retest and identify hybrid F1 again +彩色叶+紫色种皮 ; (6) Construction of the sterile line S5 for both nutritional and ornamental purposes +彩色叶+紫色种皮 , Restoration R3 +彩色叶 New hybrid rice varieties prepared by the method +彩色叶+紫色种皮 Production system, finally choose hybrid rice with both nutritional and ornamental value +彩色叶+紫色种皮 .

2. The method for breeding hybrid rice with both nutritional and ornamental properties as claimed in claim 1, characterized in that: In step (3), the planting generation number is ≥ 6.

3. The method for breeding hybrid rice with both nutritional and ornamental properties as claimed in claim 1, characterized in that: In step (4), the evaluation indicators of strong hybrid vigor include: seed coat color, yield, rice quality and disease resistance; among which, the seed coat color is purple, the yield is ≥600kg / mu, the rice quality is the rice edible quality reaching the ministerial level three or above standard, and the disease resistance is moderate resistance to rice blast.

4. The method for breeding hybrid rice with both nutritional and ornamental properties as claimed in claim 1, characterized in that: In step (5), the criteria for judging whether the ornamental plant is ornamental is that the growth period, plant height and lodging resistance are all higher than those of the parent.

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