A cell incubator with a microscopic imaging system

Abstract

The present invention relates to an incubator with a microscopic imaging system, comprising: a box body with a heating function, a box cover, a temperature and humidity adjustment device arranged on one side of the box body and outside the box body, a culture dish support table arranged inside the box body, a microscopic device arranged at the bottom of the culture dish support table, and an illumination device located above the culture dish support table; a side of the box body connected to the temperature and humidity adjustment device is regularly arranged with mesh holes penetrating through its side wall; the mesh holes are located above the culture dish support table; the temperature and humidity adjustment device exchanges gas with the inside of the box body through the mesh holes, and is used to assist in adjusting the temperature and humidity inside the box body. The structure of the present invention is simple, with a high degree of automation, suitable for cell culture operations of closed culture dishes, and effectively improves the efficiency of cell culture operations.

Description

Technical Field

[0001] The present invention relates to a cell incubator, and more particularly to a cell incubator with a microscopic imaging system. Background Art

[0002] Cell culture is a method for studying the behavior of animal cells established in the early 20th century. It refers to taking cells from tissues in vivo, simulating the in vivo growth environment, and enabling the cells to grow and reproduce under aseptic, appropriate temperature, pH, and nutritional conditions, while maintaining their structure and function. Cell preparation refers to the process by which researchers cultivate a high-purity end product of target cells using a relatively mature cell culture protocol, and which can be directly applied to the scientific research and clinical fields after passing functional and quality tests.

[0003] The operations such as feeding and draining during the cell preparation process are crucial. Currently, the above-mentioned feeding and draining operations are usually achieved by manual operation during the cell preparation process. However, this operation often requires repeatedly taking out and opening the cell culture dish and then replacing the liquid inside it, resulting in slow replacement efficiency. Moreover, traditional cell incubators can only provide a constant temperature and humidity environment, and do not have the function of assisting in replacing the liquid in the culture dish. In addition, during the cell culture process, it is also necessary to manually open the incubator to observe the growth state of the cells in the culture dish, which is not only inefficient but also often easily leads to the destruction of the environment inside the incubator, which is not conducive to the full-cycle culture of cells. Summary of the Invention

[0004] The purpose of the present invention is to provide a cell incubator with a microscopic imaging system.

[0005] To achieve the above-mentioned invention purpose, the present invention provides a cell incubator with a microscopic imaging system, including: a box body with a heating function, a box cover, a temperature and humidity regulating device arranged on one side of the box body and outside the box body, a culture dish support platform arranged inside the box body, a microscopic device arranged at the bottom of the culture dish support platform, and an illumination device located above the culture dish support platform;

[0006] On one side of the box body connected to the temperature and humidity regulating device, a series of mesh holes penetrating its side wall are regularly arranged;

[0007] The mesh holes are located above the culture dish support platform;

[0008] The temperature and humidity regulating device exchanges gas with the inside of the box body through the mesh holes, and is used to assist in regulating the temperature and humidity inside the box body.

[0009] According to one aspect of the present invention, one side of the box cover is detachably and rotatably connected to the upper end of the side of the box body where the temperature and humidity regulating device is connected;

[0010] The lighting device is installed on the inner side surface of the box cover.

[0011] According to one aspect of the present invention, an annular sealing and heat insulating strip is provided at the upper end of the box body;

[0012] The cross-sectional shape of the annular sealing and heat insulating strip is semi-circular or semi-elliptical;

[0013] On the side of the annular sealing and heat insulating strip away from the box body, at least one annular groove coaxial with the annular sealing and heat insulating strip is provided;

[0014] On the side edge of the annular sealing and heat insulating strip corresponding to the position where it is rotationally connected to the box body and the box cover, a notch for the pipeline to pass through is provided.

[0015] According to one aspect of the present invention, the box body is a hollow structure surrounded by multi-layer plates;

[0016] The box body includes: a heat conducting layer, a heating layer, a heat insulating layer, and an insulating layer, which are arranged in sequence from inside to outside;

[0017] The heating layer is used to heat the heat conducting layer, and the heat conducting layer is used to transfer the heat generated by the heating layer to the inside of the box body;

[0018] The heating layer is coated on the outside of the heat conducting layer, the heat insulating layer is coated on the outside of the heating layer, and the insulating layer is coated on the outside of the heating layer;

[0019] The position where the mesh holes are provided on the heat conducting layer is covered with a sterilizing and air-permeable film;

[0020] The box cover is a hollow plate-like body, and the side adjacent to the box body is made of a heat insulating material.

[0021] According to one aspect of the present invention, the petri dish support platform includes: a connection seat supported on the bottom of the box body, a lifting seat connected to the connection seat, a driving device, and a detection device for detecting the internal state of the petri dish installed on the lifting seat;

[0022] The driving device is supported on the connection seat;

[0023] One end of the lifting seat is a rotation connection end rotationally connected to the connection seat, and the other end is a lifting connection end connected to the driving device;

[0024] Under the driving action of the driving device, the lifting connection end can move up and down relative to the connection seat.

[0025] According to one aspect of the present invention, the connection seat includes: a connection frame body and a bottom support provided on the lower side of the connection frame body;

[0026] The lifting seat is located inside the connecting frame body and above the bottom support;

[0027] The lifting seat is of a frame structure, and a vessel mounting position is arranged thereon;

[0028] The vessel mounting position includes: a first hollow part and a second hollow part that are communicated with each other;

[0029] The second hollow part has an opening at the end of the rotation connection end of the lifting seat;

[0030] In the width direction of the lifting seat, through light-transmitting channels are arranged on opposite sides of the lifting seat at the vessel mounting position;

[0031] The light-transmitting channels are arranged at positions of the first hollow part close to the lifting connection end of the lifting seat.

[0032] According to one aspect of the present invention, the driving device includes: a driver for outputting a linear displacement, and a fork arm connected to the driver;

[0033] The fork arm includes: a driver connection arm and a lifting seat connection arm;

[0034] The lifting seat connection arm is arranged perpendicular to the driver connection arm;

[0035] A linear channel is arranged on the bottom support;

[0036] One end of the lifting seat connection arm far from the driver connection arm passes through the linear channel and is hinged to the lifting connection end of the lifting seat;

[0037] The lifting seat connection arm is located on the lower side of the bottom support, and one side far from the driver connection arm is hinged to the movable end of the driver;

[0038] A rubber damping structure is arranged at the hinged position of the driver connection arm and the driver for silent rotation of the hinged position;

[0039] A position sensor for detecting the moving position of the driver connection arm and a limit structure for limiting the maximum displacement of the driver connection arm are embedded in the driver connection arm.

[0040] According to one aspect of the present invention, the microscopic device includes: a focusing unit, an optical lens mounted on the focusing unit, and a microscopic imaging unit for receiving an image in the optical lens;

[0041] The focusing unit is mounted on the bottom support of the connecting seat;

[0042] The microscopic imaging unit is located below the bottom support.

[0043] According to one aspect of the present invention, the microscopic imaging unit is installed at the bottom of the box body, and the microscopic imaging unit is located outside the box body;

[0044] The gear drive of the focusing unit is installed at the bottom of the box body, and the gear drive is located outside the box body.

[0045] According to one aspect of the present invention, the microscopic device further includes: a first planar displacement structure;

[0046] The focusing unit is connected below the bottom support through the first planar displacement structure;

[0047] The first planar displacement structure is used to drive the focusing unit to linearly reciprocate within a horizontal plane;

[0048] A second planar displacement structure is provided in the lighting device for driving the lighting device to linearly reciprocate within a horizontal plane;

[0049] The first planar displacement structure and the second planar displacement structure are linked.

[0050] According to one solution of the present invention, the structure of the present invention is simple, the degree of automation is high, it is suitable for cell culture operations in a closed culture dish, and effectively improves the efficiency of cell culture operations.

[0051] According to one solution of the present invention, the incubator of the present invention can realize automatic auxiliary replacement of the internal liquid of the culture dish and real-time visual monitoring of the culture dish, and the corresponding operations can be realized without opening the incubator, which can effectively ensure the stability of the internal environment of the incubator during the whole cell culture cycle and improve the efficiency and safety of cell culture.

[0052] According to one solution of the present invention, the structure of the cell incubator of the present invention is simple, and it can directly control the temperature and humidity inside the box through the mesh holes opened on the box wall by the temperature and humidity adjustment device installed on one side. In addition, by directly installing the temperature and humidity adjustment device on the box wall of the box body, the ventilation path is effectively shortened and the ventilation pipeline is eliminated, realizing precise control of the temperature and humidity inside the box body.

[0053] According to one solution of the present invention, setting an annular sealing strip between the box body and the box cover can effectively eliminate the gap between the upper end surface of the box body and the box cover, which is beneficial to maintaining the stability of the constant temperature and humidity environment inside the box.

[0054] According to one solution of the present invention, by providing a notch on the annular sealing and heat-insulating strip, the installation and passage of the culture dish pipeline can be achieved through the notch. In this way, the periphery of the culture dish pipeline can be sealed by the annular sealing and heat-insulating strip. Especially when the box cover is buckled with the box body, due to the action of the extrusion force on the sealing strip, the end face at the notch position can be in closer contact with the outer side of the pipeline, which is beneficial to ensuring the airtightness around the pipeline. In addition, the notch on the sealing strip can also achieve the positioning effect of the pipeline, and no additional clamping structure is required, effectively simplifying the installation of the pipeline in the box body.

[0055] Through the above settings, by providing a notch on the annular sealing and heat-insulating strip, it can conveniently and simply replace the method of opening an opening on the box body or the box cover, and thus can effectively eliminate the drawback of the poor airtightness of the structure caused by opening an opening on the box body or the box cover. In addition, the method of opening an opening on the sealing ring is simple and convenient and does not require adding an additional sealing structure. Therefore, while ensuring good airtightness, the structure is effectively simplified.

[0056] According to one solution of the present invention, the provided microscopic device and lighting device are beneficial for comprehensively and clearly obtaining the cell growth state in the culture dish, ensuring the comprehensive and accurate grasp of the information throughout the cell growth cycle, so that relevant information can be further provided to the processing system to achieve precise adjustment of substances such as nutrient solution in the culture dish, effectively improving the operation efficiency of cells.

[0057] According to one solution of the present invention, the temperature and humidity adjustment device of the present invention is provided with an air purification unit, which can disinfect and purify the environment inside the box while ensuring that the temperature and humidity inside the box are within the preset conditions, so as to ensure the cleanliness of the entire box environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 is a schematic perspective view of a cell incubator according to an embodiment of the present invention;

[0059] Figure 2 is a schematic internal structure diagram of a cell incubator according to an embodiment of the present invention;

[0060] Figure 3 is a schematic top view of a cell incubator according to an embodiment of the present invention;

[0061] Figure 4 is a schematic bottom view of a cell incubator according to an embodiment of the present invention;

[0062] Figure 5 is a schematic structure diagram of an annular sealing and heat-insulating ring according to an embodiment of the present invention;

[0063] Figure 6 is a schematic diagram showing the layout of a petri dish support platform and a microscopic device according to an embodiment of the present invention;

[0064] Figure 7 is a side view of a petri dish support platform according to an embodiment of the present invention;

[0065] Figure 8 is a structural diagram of a petri dish support platform according to an embodiment of the present invention;

[0066] Figure 9 is a bottom view of a petri dish support platform according to an embodiment of the present invention;

[0067] Figure 10 is a structural diagram of a lifting seat according to an embodiment of the present invention. Specific Embodiments

[0068] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0069] When describing the embodiments of the present invention, the orientation or positional relationships expressed by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" are based on the orientation or positional relationships shown in the relevant drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be construed as limiting the present invention.

[0070] The present invention will be described in detail below in conjunction with the drawings and specific embodiments. The embodiments cannot be described in detail one by one here, but the embodiments of the present invention are not limited to the following embodiments.

[0071] Combined with Figure 1 、 Figure 2 、 Figure 3 、 Figure 4As shown, according to an embodiment of the present invention, a cell incubator with a microscopic imaging system of the present invention includes: a box body 1 with a heating function, a box cover 2, a temperature and humidity regulating device 3 arranged on one side of the box body 1 and outside the box body 1, a culture dish support platform 4 arranged inside the box body 1, a microscopic device 5 arranged at the bottom of the culture dish support platform 4, and an illumination device 6 located above the culture dish support platform 4. In this embodiment, the box body 1 is integrally in a symmetrical rectangular structure. On one side of the box body 1 connected to the temperature and humidity regulating device 3, mesh holes 1a penetrating its side wall are regularly arranged. In this embodiment, the mesh holes 1a are symmetrically arrayed on both sides of the center line perpendicular to the length direction of the box body. In this embodiment, the mesh holes 1a are located above the culture dish support platform 4. In this embodiment, the temperature and humidity regulating device 3 exchanges gas with the inside of the box body 1 through the mesh holes 1a, and is used to assist in regulating the temperature and humidity inside the box body 1.

[0072] In this embodiment, the box body 1 has a heating function, and it can heat the internal environment to ensure that the internal environment of the box has a suitable cell culture temperature. When the temperature inside the box is relatively high, the heating of the box body 1 can be stopped and / or the temperature inside the box can be regulated by the operation of the temperature and humidity regulating device 3, so that the temperature of the internal environment of the box always remains within a reasonable range.

[0073] In this embodiment, when it is necessary to control the humidity of the internal environment of the box, the temperature and humidity regulating device 3 can be operated alone to humidify or dehumidify the internal environment of the box to keep the humidity of the internal environment of the box constant.

[0074] In this embodiment, the number of the mesh holes 1a opened on the box body 1 and the opening size are matched with the volume of the box body. Through the above-mentioned mesh holes 1a, the temperature and humidity regulating device 3 can circulate the air inside the box body 1, and no obvious air flow will be generated inside the box. Especially when the mesh holes 1a are opened in the upper half of the side wall of the box body 1 (that is, above the culture dish support platform 4), this can more effectively eliminate the air flow inside the box body and is beneficial to realizing stable gas exchange.

[0075] Through the above settings, the structure of the cell incubator of the present invention is simple. The temperature and humidity regulating device 3 installed on one side can directly assist in controlling the temperature and humidity inside the box through the mesh holes opened on the box wall. In addition, by directly installing the temperature and humidity regulating device 3 on the box wall of the box body 1, the ventilation path is effectively shortened and the ventilation pipeline is eliminated, realizing precise control of the temperature and humidity inside the box body 1.

[0076] Combined Figure 1 、 Figure 2 、 Figure 3 、 Figure 4As shown, according to an embodiment of the present invention, one side of the lid 2 is detachably and rotatably connected to the upper end of one side of the box body 1 where the temperature and humidity adjusting device 3 is connected. In this embodiment, the lid 2 is connected to the box body 1 through a detachable pin shaft. When installing structures such as petri dishes into the box body 1, the lid 2 can be detached from the box body 1, and after the installation of the petri dish is completed, the lid 2 is connected to the box body 1 to achieve the sealing of the box body 1. In this embodiment, the lighting device 6 is installed on the inner side surface of the lid 2. Among them, the lighting device 6 is detachably connected to the lid 2.

[0077] Combined with Figure 1 、 Figure 2 、 Figure 3 、 Figure 5 As shown, according to an embodiment of the present invention, an annular sealing and heat-insulating strip 11 is provided at the upper end of the box body 1. In this embodiment, the cross-sectional shape of the annular sealing and heat-insulating strip 11 is semi-circular or semi-elliptical. By setting the upper side of the annular sealing and heat-insulating strip 11 to be arc-shaped, it can achieve line contact with the lid, effectively making close contact with various positions of the lid.

[0078] Through the above settings, setting an annular sealing strip between the box body 1 and the lid 2 can effectively eliminate the gap between the upper end face of the box body and the lid, which is beneficial to ensuring the stable maintenance of the constant temperature and humidity environment inside the box.

[0079] In this embodiment, at least one annular groove 11a coaxial with the annular sealing and heat-insulating strip 11 is provided on the side of the annular sealing and heat-insulating strip 11 away from the box body 1. In this embodiment, the cross-section of the annular groove 11a is rectangular. Furthermore, the side surface of the annular groove 11a is a vertical surface, and thus a sharp corner is formed at the connection position between the upper side of the annular sealing and heat-insulating strip and the curved surface. Furthermore, a multi-sealing effect can be achieved at the position where it contacts the lid 2. In addition, the strength of the sharp corner edge formed by the annular groove on the sealing strip is small, and thus it can produce a slight bend towards the inner side of the groove at the position where it contacts the lid, and thus can have a closer contact with the lid. Moreover, while the edge produces a slight bend, it can have a relative sliding with a small distance from the lid, which can clean the position where it contacts the lid and ensure the sealing performance of the contact position. In addition, a plurality of annular grooves 11a can be arranged at intervals side by side in the width direction of the annular sealing and heat-insulating strip 11, and thus multiple high and low edges can be formed on the surface of the sealing strip to achieve a multi-sealing effect.

[0080] In this embodiment, a notch 11b for the pipeline to pass through is provided on the side edge of the annular sealing and heat-insulating strip 11 corresponding to the position where it is rotatably connected to the box body 1 and the lid 2. In this embodiment, the width and shape of the notch match the cross-sectional shape of the pipeline passing through.

[0081] With the above settings, by providing a notch 11b on the annular sealing and heat insulating strip 11, the installation and passage of the petri dish pipeline can be achieved through the notch. In this way, the periphery of the petri dish pipeline can be sealed by the annular sealing and heat insulating strip 11. Especially when the box cover is buckled with the box body, due to the extrusion force acting on the sealing strip, the end face at the position of the notch 11b can be made to contact the outer side of the pipeline more closely, which is beneficial to ensuring the airtightness around the pipeline. In addition, the notch 11b on the sealing strip can also play a role in positioning the pipeline, eliminating the need for an additional clamping structure and effectively simplifying the installation of the pipeline in the box body 1.

[0082] With the above settings, by providing a notch 11b on the annular sealing and heat insulating strip 11, the method of opening an aperture in the box body or the box cover can be conveniently and simply replaced, thereby effectively eliminating the drawback of the poor airtightness of the structure caused by opening an aperture in the box body or the box cover. In addition, the method of opening an aperture in the sealing ring is simple and convenient and does not require the addition of an additional sealing structure, thus effectively simplifying the structure while ensuring good airtightness.

[0083] Combined with Figure 1 , Figure 2 , Figure 3 , Figure 4 As shown in

[0084] In this embodiment, the position where the mesh holes 1a are provided on the heat conducting layer is covered with a sterilizing and air-permeable membrane; through the provided sterilizing and air-permeable membrane, the isolation between the inside of the box body and the external environment can be achieved. When the gas passes through the sterilizing and air-permeable membrane, not only the purification of the gas is realized, but also the air flow generated by gas exchange is inhibited.

[0085] By setting the box body as a multi-layer composite structure, its structure is simple and it can be conveniently embedded in other systems, ensuring the flexibility of use and installation of the present invention. In addition, by setting the box body to have a heating layer, it can conveniently achieve direct heating inside the box body, eliminating the intermediate heat exchange path, effectively reducing the contact with the outside world, and being beneficial to the accurate control of the temperature inside the box body. At the same time, since the temperature and humidity adjustment device 3 is directly connected to the box body, by setting a heat insulation layer and an insulating layer on the box body, the influence of the heat generated during the operation of the temperature and humidity adjustment device 3 on the internal environment of the box body can also be effectively eliminated.

[0086] In this embodiment, the box cover 2 is a hollow plate-like body, and the side adjacent to the box body 1 is made of a heat insulation material.

[0087] Through the above settings, the cooperation with the box body 1 is effectively realized, which is beneficial to ensuring the stability of the internal environment of the box body.

[0088] Combined Figure 6 、 Figure 7 、 Figure 8 、 Figure 9 As shown, according to an embodiment of the present invention, the culture dish support platform 4 includes: a connection seat 41 supported on the bottom of the box body 1, a lifting seat 42 connected to the connection seat 41, a driving device 43, and a detection device 44 for detecting the internal state of the culture dish installed on the lifting seat 42. In this embodiment, the driving device 43 is supported on the connection seat 41. One end of the lifting seat 42 is a rotational connection end rotatably connected to the connection seat 41, and the other end is a lifting connection end connected to the driving device 43. In this embodiment, under the driving action of the driving device 43, the lifting connection end can move up and down relative to the connection seat 41.

[0089] Through the above settings, the lifting seat 42 in the present invention is used to carry the culture dish (for example, an adherent cell culture dish). Furthermore, through the action of the driving device, the lifting connection end of the lifting seat can perform a vertical lifting action with the rotational connection end as the axis, so that the lifting seat 42 generates an inclined movement relative to the connection seat 41, thereby promoting the smooth outflow of the liquid in the culture dish when it needs to be replaced, and avoiding residues in the culture dish.

[0090] Through the above settings, the present invention can automatically realize the lifting operation of the lifting seat without manual participation, which can effectively improve the liquid renewal efficiency in the culture dish.

[0091] Combined Figure 6 、 Figure 7 、 Figure 8 、 Figure 9As shown, according to an embodiment of the present invention, the connecting seat 41 includes: a connecting frame body 411 and a bottom support 412 provided on the lower side of the connecting frame body 411. In this embodiment, the connecting frame body 411 is a continuous rectangular frame body, and its size can be adaptively adjusted according to the size inside the box body. In this embodiment, the bottom support 412 is a plate-like body, and it is fixedly installed on the lower side of the connecting frame body 411. In this embodiment, the bottom support 412 and the connecting frame body 411 can be integrally provided, or can be fixed by means such as bonding and screw connection.

[0092] In this embodiment, a plurality of feet are provided at intervals on the lower side of the connecting frame body 411. The height of the feet can be adjusted as needed. In this embodiment, the connecting frame body 411 is installed on the bottom of the box body 1 through the feet. In this embodiment, the feet can be fixed to the bottom of the box body 1 through a connecting member to realize the fixed installation of the culture dish support table 4.

[0093] Combined Figure 6 , Figure 7 , Figure 8 , Figure 9 As shown, according to an embodiment of the present invention, the lifting seat 42 is located inside the connecting frame body 411 and above the bottom support 412. In this embodiment, rotating shafts 42a for rotatably connecting with the connecting frame body 411 are respectively provided on opposite sides of the rotating connection end of the lifting seat 42. The rotating shafts 42a are connected through bearings installed on the lifting seat 42 and the connecting frame body 411 to realize the flexible rotation of the lifting seat 42 relative to the connecting frame body 411. In this embodiment, in order to realize the connection of the lifting seat 42 inside the connecting frame body 411, the outer shape of the lifting seat 42 is adapted to the inner shape of the connecting frame body 411. If the inside of the connecting frame body 411 is rectangular, the outer shape of the lifting seat 42 should be set to be rectangular to match it.

[0094] Combined Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10As shown, according to an embodiment of the present invention, the lifting seat 42 is a frame structure, which has a vessel mounting position 42b for mounting a culture dish. In this embodiment, the lifting seat 42 is a hollow frame structure, and the vessel mounting position 42b for the culture dish thereon is formed by the hollow structure on the lifting seat 42. Specifically, the vessel mounting position 42b includes: a first hollow portion 42b1 and a second hollow portion 42b2 that communicate with each other. In this embodiment, along the direction from the rotation connection end to the lifting connection end of the lifting seat 42 (i.e., the length direction), the second hollow portion 42b2 and the first hollow portion 42b1 are arranged in sequence, wherein the second hollow portion 42b2 has an opening at the end of the rotation connection end of the lifting seat 42. In this embodiment, the widths of the first hollow portion 42b1 and the second hollow portion 42b2 are different, so that a stepped structure is formed on the vessel mounting position 42b, which is beneficial to the installation and positioning of the culture dish in the first hollow portion 42b1, ensuring the installation stability of the culture dish, while the second hollow portion 42b2 corresponds to structures such as the bottleneck and the bottle cap on the culture dish, realizing the stable installation of the entire culture dish.

[0095] In this embodiment, the second hollow portion 42b2 forms an opening at the end of the rotation connection end, which can further avoid interference with structures such as the bottleneck and the bottle cap on the culture dish, and can adapt to the installation of bottlenecks and bottle caps of different sizes, and during the lifting process of the lifting seat 42, it provides sufficient space to avoid interference between the entire culture dish support 4 and the culture dish.

[0096] Through the above settings, the vessel mounting position 42b is set as a hollow structure, which can effectively reduce the positions where there is interference with the culture dish, and is beneficial to the stable installation of the culture dish. In addition, by setting the vessel mounting position 42b as hollow, the mass of the lifting seat can be greatly reduced, the structure of the lifting seat can be simplified, which is beneficial to ensuring the long-term stable operation of the present invention. In addition, by setting the hollow vessel mounting position 42b, it is also beneficial to install other optical devices on the culture dish support 4 of the present invention for real-time observation of the state in the culture dish, effectively improving the use flexibility of the present invention.

[0097] In this embodiment, an arc surface is provided on the lower side of the end of the rotation connection end of the lifting seat 42, and this arc surface is used to connect the end face and the lower side face of the rotation connection end, so as to form a transition part at the end position.

[0098] Through the above settings, an arc surface is provided on the lower side of the end of the rotation connection end of the lifting seat 42, which is beneficial to ensuring the smooth rotation of the lifting seat 42 and further ensuring the use stability of the present invention.

[0099] Combined with Figure 6 、 Figure 7 、 Figure 8 、Figure 9 As shown, according to an embodiment of the present invention, in the width direction of the lifting seat 42, through light-transmitting channels 421 are provided on opposite sides of the vessel mounting position 42b of the lifting seat 42; in this embodiment, the light-transmitting channels 421 are provided at positions close to the lifting connection end of the first hollow part 42b1 of the lifting seat 42. In this embodiment, the light-transmitting channels 421 are coaxially provided on the frame of the lifting seat 42. Furthermore, when the culture dish is fixed, the light-transmitting channels 421 can be coaxially provided on opposite sides of the culture dish. Thus, by shining light through the light-transmitting channel 421 on one side, it can pass through the culture dish and reach the light-transmitting channel 421 on the other side and be transmitted out, thereby enabling the collection of the internal state of the culture dish (such as the internal environment, cell growth state, contamination situation, turbidity of the nutrient solution, etc.) for subsequent detection and analysis.

[0100] In this embodiment, in the thickness direction of the lifting seat 42, the light-transmitting channels 421 are provided at positions close to the lower side of the lifting seat 42, that is, the distance between the light-transmitting channels 421 and the lower side is less than the distance between the connection channels and the upper side.

[0101] Through the above settings, by providing the light-transmitting channels 421 at positions close to the lifting connection end of the lifting seat 42, it can be used to detect the internal environment at the rear of the culture dish in real time to accurately detect the internal environment of the culture dish.

[0102] Combined Figure 6 、 Figure 7 、 Figure 8 、 Figure 9 、 Figure 10 As shown, according to an embodiment of the present invention, a support structure 422 is provided at the bottom of the lifting seat 42. In this embodiment, the support structure 422 includes: a first support structure 4221 and a second support structure 4222. The first support structure 4221 is provided along the inner edge of the bottom of the first hollow part 42b1; the second support structure 4222 is connected to partial inner edges on opposite sides of the bottom of the second hollow part 42b2. In this embodiment, the first support structure 4221 is a strip-shaped structure, which is provided continuously or discontinuously along the inner edge of the bottom of the first hollow part 42b1. In this embodiment, the second support structure 4222 is a plate-like body, and its opposite ends are respectively fixedly connected to partial inner edges on opposite sides of the bottom of the second hollow part 42b2.

[0103] Through the above settings, by providing the support structure 422 at the bottom edge of the vessel mounting position 42b, it can effectively support the edge and bottleneck part of the culture dish.

[0104] In this embodiment, the second support structure 4222 is provided at an interval from the end opening of the rotation connection end.

[0105] With the above settings, the gap between the second support structure 4222 and the end opening of the rotating connection end can play an avoidance role, avoiding interference with other structures, which is beneficial to ensuring the normal and stable operation of the present invention.

[0106] Combined Figure 6 , Figure 7 , Figure 8 , Figure 9 As shown in FIGS.

[0107] Combined Figure 6 , Figure 7 , Figure 8 , Figure 9 As shown in FIGS.

[0108] In this embodiment, the lifting seat connecting arm 4322 is located below the bottom support 412, and the side away from the driver connecting arm 4321 is hinged to the movable end of the driver 431. In this embodiment, a first notch is provided on the bottom support 412 below the rotating connection end of the lifting seat 42, and a second notch is provided at a position of the connection frame 411 of the connection seat 41 corresponding to the first notch. The second notch is communicated with the first notch, so that the driving device 43 can be installed at the positions of the first notch and the second notch.

[0109] In this embodiment, the driver 431 is a linear driving device, and its telescopic end can perform telescopic movement along the linear direction. Therefore, by hinging the free end of the driver 431 to the driver connecting arm 4321, the pushing effect on the lifting seat 42 can be realized.

[0110] According to an embodiment of the present invention, the actuator 431 is implemented by an electric cylinder, which can accurately control the displacement of its telescopic end through an electric signal, and can automatically and accurately control the tilt angle of the lifting seat through the control of the electric signal, thereby realizing the micro-flow of the nutrient solution in the culture dish and the flexible adjustment of the horizontal position of the nutrient solution.

[0111] With the above arrangement, the actuator connecting arm 4321 is arranged on the lower side of the bottom support 412, while the lifting seat 42 is located on the upper side of the bottom support 412. During the process of driving the lifting seat 42 to move up and down through the connection between the actuator 431 and the actuator connecting arm 4321, the bottom support 412 can play a role in supporting, limiting and guiding the actuator connecting arm 4321, which is beneficial to the stable operation of the actuator 431. In addition, with the above arrangement, by controlling the telescopic length of the free end of the actuator 431, the tilt angle of the lifting seat 42 can be accurately controlled flexibly, and this can be achieved without other complex structures, realizing the advantages of high operation accuracy, good stability and simple structure.

[0112] In this embodiment, a rubber damping structure is provided at the hinged position between the actuator connecting arm 4321 and the actuator 431 for silent rotation at the hinged position. In this embodiment, a damping structure made of rubber can be provided on both sides of the hinged position or on the hinge shaft to achieve noise reduction at the hinged position, and it also effectively ensures the smooth and stable operation and is beneficial to ensuring the accurate operation position.

[0113] In this embodiment, a position sensor for detecting its moving position is embedded in the actuator connecting arm 4321. By the real-time detection of the position of the actuator connecting arm 4321 by the position sensor, the accurate control of the position of the fork arm 32 can be achieved, and then the accurate control of the tilt angle of the lifting seat can be achieved.

[0114] In this embodiment, a limiting structure for limiting the maximum displacement of the actuator connecting arm 4321 is also provided on the actuator connecting arm 4321. By this limiting structure, the excessive movement of the actuator connecting arm 4321 can be effectively avoided, and then the maximum rising height of the lifting seat 42 can be effectively suppressed, which is beneficial to ensuring the stable operation of the lifting seat and eliminating interference with other structures.

[0115] Combined with Figure 6 、 Figure 7As shown, according to an embodiment of the present invention, a detection device 44 for detecting the internal state of the culture dish installed on the lifting seat 42 is provided on the connection frame 411. In this embodiment, the detection device adopts an opposed detection device. Specifically, the detection device 44 includes: a laser light source 441, an optical fiber 442, and an optical receiver 443. In this embodiment, on opposite sides of the connection frame 411, mounting through-holes penetrating the frame are respectively provided, and thus the laser light source 441 and the optical receiver 443 can be oppositely mounted in the mounting through-holes. The optical fiber 442 is respectively embedded in the light-transmitting channel 421 of the lifting seat 42; when the lifting seat 42 is horizontally located in the connection frame 411, the laser light source 441, the optical fiber 442, and the optical receiver 443 are arranged in alignment, and the light emitted by the laser light source 441 can be transmitted to the optical receiver 443 through the optical fiber 442, and thus the detection of the environment in the culture dish can be realized. When the lifting seat 42 is tilted, since the lifting connection end leaves the connection frame 411, the detection device 44 can output a signal for the lifting seat 42 to rise, and the environment in the culture dish cannot be detected. In this embodiment, the optical receiver 443 can process and analyze the received signal by itself, or can transmit it to other devices for processing and analysis, which can be set according to actual applications.

[0116] Through the above settings, the automatic detection of the interior of the culture dish of the present invention can be realized by using the detection device to detect the culture dish. In addition, the automatic control of the lifting seat can also be realized through the on-off of the detection device, improving the control accuracy of the present invention.

[0117] In addition, according to needs, the optical fiber 442 can also be arranged to abut against the culture dish, which can provide a certain pre-tightening effect on the culture dish, beneficial to the reliable fixation of the culture dish, and this setting method is more beneficial when the lifting seat is in an inclined state. Through the abutting pre-tightening effect on the rear part of the culture dish, the problem that the liquid in the culture dish accumulates at the bottleneck end and causes the culture dish to tip over can be effectively avoided, making the operation of the present invention more reliable. In addition, the optical fiber 442 and the culture dish can also be integrally arranged to eliminate the influence of the contact position on the detection result, and further play a role in fixedly connecting the culture dish.

[0118] As Figure 6 shown, according to an embodiment of the present invention, a first opening penetrating the bottom support 412 is provided on the bottom support 412. In this embodiment, the first opening is located below the first hollow part 42b1. In this embodiment, the size of the first opening can be adjusted according to needs to adapt to the installation of different structures.

[0119] Combined Figure 2 、 Figure 4 、 Figure 6 、 Figure 9As shown, according to an embodiment of the present invention, a microscopic device 5 includes: a focusing unit 51, an optical lens mounted on the focusing unit 51, and a microscopic imaging unit 52 for receiving the image in the optical lens. In this embodiment, the focusing unit 51 is mounted on the bottom support 412 of the connection base 41, and the microscopic imaging unit 52 is located below the bottom support 412. In this embodiment, the focusing unit 51 is located on the lower side of the bottom support 412, and it includes: a lens holder 511, a gear set 512 for adjusting the vertical position of the lens holder 511, and a gear drive 513 for driving the gear set 512 to rotate. In this embodiment, the lens holder 511 is at the position of the first opening of the bottom support 412, and it focuses and images the internal state of the transparent culture dish through the mounted optical lens for transmission to the microscopic imaging unit 52.

[0120] Combined with Figure 2 、 Figure 4 、 Figure 6 、 Figure 9 As shown, according to an embodiment of the present invention, the microscopic imaging unit 52 is mounted on the bottom of the box body 1, and the microscopic imaging unit 52 is located outside the box body 1. In this embodiment, a second opening is provided at the bottom of the box body 1 for transmitting the image in the optical lens to the microscopic imaging unit 52. In this embodiment, the position where the microscopic imaging unit 52 is connected to the second opening needs to be sealed.

[0121] In this embodiment, the gear drive 513 of the focusing unit 51 is mounted on the bottom of the box body 1, and the gear drive 513 is located outside the box body 1.

[0122] In this embodiment, the focusing method of the focusing unit 51 through the gear set 512 is not only structurally compact but also has high focusing accuracy, while the microscopic imaging unit 52 adopts a compact microscopic imaging structure, which is small in volume and has little impact on the space required for the installation of the entire cell incubator, ensuring the installation flexibility of the cell incubator of the present invention.

[0123] According to an embodiment of the present invention, the microscopic device 5 further includes: a first planar displacement structure.

[0124] In this embodiment, the focusing unit 51 is connected below the bottom support 412 through the first planar displacement structure; the first planar displacement structure is used to drive the focusing unit 51 to linearly reciprocate in the horizontal plane. In this embodiment, the first planar displacement structure includes: a first X-axis drive and a first Y-axis drive, the first Y-axis drive is supported on the first X-axis drive, and the focusing unit is mounted on the first Y-axis drive, and the reciprocating movement of the focusing unit in the horizontal direction is realized through the combined action of the first X-axis drive and the first Y-axis drive.

[0125] In this embodiment, to ensure the movement range of the focusing unit, the size of the first opening can be set as a relatively large opening area according to this movement range, so as to ensure that the focusing unit can perform focusing detection on various positions in the culture dish.

[0126] In this embodiment, to ensure that the image formed by the focusing unit is stably and effectively input into the microscopic imaging unit 52, the microscopic device 5 further includes: a prism structure for transmitting the image. In this embodiment, the prism structure includes: a first prism supported on the first planar displacement structure, a second prism supported at the second opening position, and an intermediate prism group for transmitting the image in the first prism to the second prism. Through the above-set prism structure, the image formed by the optical lens in the focusing unit 51 can be stably input into the microscopic imaging unit 52.

[0127] In this embodiment, a second planar displacement structure is provided in the lighting device 6 for driving the lighting device 6 to linearly reciprocate within a horizontal plane. In this embodiment, the second planar displacement structure includes: a second X-axis drive and a second Y-axis drive. The second Y-axis drive is supported on the second X-axis drive, and the focusing unit is installed on the second Y-axis drive. Through the combined action of the second X-axis drive and the second Y-axis drive, the reciprocating movement of the focusing unit in the horizontal direction is realized.

[0128] In this embodiment, the first planar displacement structure and the second planar displacement structure are linked.

[0129] Through the above settings, by setting the lighting device and the focusing unit to be linked, clear imaging of various parts of the culture dish is effectively realized, which is beneficial to comprehensively and clearly obtaining the cell growth state in the culture dish, ensuring the comprehensive and accurate grasp of the information of the entire cell growth cycle, and thus further providing relevant information to the processing system to realize the precise adjustment of the nutrient solution, etc. in the culture dish.

[0130] According to an embodiment of the present invention, the temperature and humidity adjustment device 3 includes: an air conditioning unit and an air purification unit. In this embodiment, the air conditioning unit is used to assist in adjusting the temperature and humidity of the environment inside the box body 1, and the air purification unit is used to further disinfect and purify the environment inside the box body to further ensure the cleanliness of the entire box body environment.

[0131] According to an embodiment of the present invention, a carbon dioxide sensor, an oxygen sensor, a humidity sensor, and a temperature sensor are provided in the box body 21 for real-time detection of the internal environment of the box body 21. Furthermore, through the temperature and humidity adjustment device 23 and the heating layer of the box body, the real-time and accurate adjustment of the internal environment of the box body can be realized, ensuring the balance of the temperature and humidity inside the box body and avoiding the occurrence of a gradient difference in the box body.

[0132] The above content is only an example of the specific solution of the present invention. For the equipment and structures not described in detail therein, it should be understood that the existing general equipment and general methods in the art are adopted for implementation.

[0133] The above is only one solution of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A cell incubator with a microscopic imaging system, characterized in that, it includes: a box body (1) with a heating function, a box cover (2), a temperature and humidity adjusting device (3) arranged on one side of the box body (1) and outside the box body (1), a culture dish support platform (4) arranged inside the box body (1), a microscopic device (5) arranged at the bottom of the culture dish support platform (4), and an illumination device (6) located above the culture dish support platform (4); On the side of the box body (1) connected to the temperature and humidity adjusting device (3), mesh holes (1a) penetrating through its side wall are regularly arranged; The mesh holes (1a) are located above the culture dish support platform (4); The temperature and humidity adjusting device (3) exchanges gas with the interior of the box body (1) through the mesh holes (1a) to assist in adjusting the temperature and humidity inside the box body (1); The culture dish support platform (4) includes: a connection seat (41) supported on the bottom of the box body (1), a lifting seat (42) connected to the connection seat (41), a driving device (43), and a detection device (44) for detecting the internal state of the culture dish installed on the lifting seat (42); The driving device (43) is supported on the connection seat (41); One end of the lifting seat (42) is a rotation connection end rotatably connected to the connection seat (41), and the other end is a lifting connection end connected to the driving device (43); Under the driving action of the driving device (43), the lifting connection end can move up and down relative to the connection seat (41); The connection seat (41) includes: a connection frame body (411) and a bottom support (412) arranged on the lower side of the connection frame body (411); The lifting seat (42) is provided with a vessel installation position (42b); In the width direction of the lifting seat (42), through light transmission channels (421) are arranged on opposite sides of the vessel installation position (42b) of the lifting seat (42); The driving device (43) includes: a driver (431) for outputting a linear displacement, and a fork arm (432) connected to the driver (431); The fork arm (432) includes: a driver connection arm (4321) and a lifting seat connection arm (4322); The lifting seat connection arm (4322) is arranged perpendicular to the driver connection arm (4321); A linear channel (4121) is arranged on the bottom support (412); One end of the lifting seat connection arm (4322) far from the driver connection arm (4321) passes through the linear channel (4121) and is hinged to the lifting connection end of the lifting seat (42); The lifting seat connection arm (4322) is located under the bottom support (412), and one side far from the driver connection arm (4321) is hinged to the movable end of the driver (431); A rubber damping structure is arranged at the hinged position of the driver connection arm (4321) and the driver (431) for quiet rotation at the hinged position; The drive connecting arm (4321) is embedded with a position sensor for detecting its moving position and a limit structure for limiting the maximum displacement of the drive connecting arm (4321); The connecting frame body (411) is provided with a detection device (44) for detecting the internal state of the culture dish; wherein, the detection device (44) includes: a laser light source (441), an optical fiber (442), and an optical receiver (443); On opposite sides of the connecting frame body (411), mounting through holes penetrating the frame body are respectively provided to oppositely mount the laser light source (441) and the optical receiver (443) in the mounting through holes; The optical fiber (442) is respectively embedded in the light-transmitting channel (421) of the lifting seat (42); When the lifting seat (42) is located in the connecting frame body (411), the laser light source (441), the optical fiber (442), and the optical receiver (443) are aligned, and the light emitted by the laser light source (441) can be transmitted to the optical receiver (443) through the optical fiber (442) to realize the detection of the environment in the culture dish.

2. The cell incubator according to claim 1, characterized in that, One side of the box cover (2) is detachably and rotatably connected to the upper end of one side of the box body (1) where the temperature and humidity adjustment device (3) is connected; The lighting device (6) is installed on the inner side surface of the box cover (2).

3. The cell incubator according to claim 2, characterized in that, An annular sealing and heat-insulating strip (11) is provided at the upper end of the box body (1); The cross-sectional shape of the annular sealing and heat-insulating strip (11) is semi-circular or semi-elliptical; At least one annular groove (11a) coaxial with the annular sealing and heat-insulating strip (11) is provided on the side of the annular sealing and heat-insulating strip (11) away from the box body (1); A notch (11b) for a pipeline to pass through is provided on the side edge of the annular sealing and heat-insulating strip (11) corresponding to the position where it is rotatably connected to the box body (1) and the box cover (2).

4. The cell incubator according to claim 3, characterized in that, The box body (1) is a hollow structure surrounded by multi-layer plates; The box body (1) includes: a heat-conducting layer, a heating layer, a heat-insulating layer, and an insulating layer arranged in sequence from the inside to the outside; The heating layer is used to heat the heat-conducting layer, and the heat-conducting layer is used to transfer the heat generated by the heating layer to the inside of the box body (1); The heating layer is coated on the outside of the heat-conducting layer, the heat-insulating layer is coated on the outside of the heating layer, and the insulating layer is coated on the outside of the heating layer; A germicidal and air-permeable film is covered at the position where the mesh holes (1a) are provided on the heat-conducting layer; The box cover (2) is a hollow plate-shaped body, and the side adjacent to the box body (1) is made of a heat-insulating material.

5. The cell incubator according to claim 4, characterized in that, The microscope device (5) includes: a focusing unit (51), an optical lens mounted on the focusing unit (51), and a microscopic imaging unit (52) for receiving an image in the optical lens; The focusing unit (51) is mounted on the bottom support (412) of the connection base (41); The microscopic imaging unit (52) is located below the bottom support (412).

6. The incubator for cell culture according to claim 5, characterized in that the microscopic imaging unit (52) is mounted on the bottom of the box body (1), and the microscopic imaging unit (52) is located outside the box body (1); the gear drive (513) of the focusing unit (51) is mounted on the bottom of the box body (1), and the gear drive (513) is located outside the box body (1).

7. The incubator for cell culture according to any one of claims 5 to 6, characterized in that the microscope device (5) further includes: a first planar displacement structure; the focusing unit (51) is connected below the bottom support (412) through the first planar displacement structure; the first planar displacement structure is used to drive the focusing unit (51) to linearly reciprocate in a horizontal plane; a second planar displacement structure is provided in the lighting device (6) for driving the lighting device (6) to linearly reciprocate in a horizontal plane; the first planar displacement structure and the second planar displacement structure are linked.

8. The incubator for cell culture according to claim 1, characterized in that the lifting seat (42) is located inside the connection frame body (411) and above the bottom support (412); the lifting seat (42) is of a frame structure; the vessel mounting position (42b) includes: a first hollow part (42b1) and a second hollow part (42b2) that communicate with each other; the second hollow part (42b2) has an opening at the end of the rotation connection end of the lifting seat (42); the light transmission channel (421) is provided at a position of the first hollow part (42b1) close to the lifting connection end of the lifting seat (42).

Images