A wound repair gel, a wound autologous blood repair gel and a method of preparation

Abstract

A kind of wound repair gel, wound autologous blood repair gel and preparation method.The preparation material of the repair gel includes: the composite amino acid, glucose, polyvinyl alcohol, insulin are added according to weight ratio;Polyvinylpyrrolidone, gel additive are added according to volume ratio.The technical scheme provided in the application can effectively promote the rapid repair of wound cells, improve the speed of wound healing, and improve the effect of wound repair.

Description

Technical Field

[0001] This invention relates to a wound repair gel, belonging to the field of wound repair technology; this invention also relates to an autologous blood wound repair gel; this invention also relates to a method for preparing a wound repair gel; this invention also relates to a method for preparing an autologous blood wound repair gel. Background Technology

[0002] Chronic, non-healing wounds are caused by various factors leading to the destruction of skin integrity and normal tissue, as well as the loss of skin function. In recent years, with the aging of the global population and the increasing prevalence of diabetes, vascular diseases, and other chronic diseases in the elderly, the incidence of non-healing wounds has been rising, becoming a significant chronic disease seriously affecting people's health. Statistics show that approximately 1% of patients worldwide suffer from non-healing wounds, and their medical expenses account for up to 5% of total medical costs. Because chronic, non-healing wounds are slow to heal, they severely impact the recovery from the primary disease and the patient's quality of life, placing a heavy burden of care and finances on families. A small number of chronic, non-healing wounds can ulcerate and become cancerous. If infection spreads from a chronic, non-healing wound, it can lead to complications such as sepsis, worsening the primary disease and even endangering the patient's life. The pathogenesis of chronic, non-healing wounds is complex and not yet fully understood. Clinically, there are many treatment methods for chronic, non-healing wounds, with local treatment being one of the main approaches, but the clinical efficacy is not always satisfactory. Treatment for chronic, non-healing wounds mainly involves anti-infection measures, improving nutrition, and promoting wound healing.

[0003] Currently, in addition to traditional treatments, there are many clinical treatment methods and approaches for chronic wounds. These include negative pressure wound therapy, moist burn ointment, platelet-rich plasma (PRP), and newer treatments such as growth factors, cytokine inhibitors and stimulants, skin substitutes, gene and stem cell therapy, extracellular matrix and angiogenesis stimulants, and matrix metalloproteinase inhibitors. However, these new treatments are not yet widely available, are costly, and have a low cost-effectiveness ratio.

[0004] Therefore, how to provide a wound repair gel that can effectively promote the rapid repair of wound cells, improve the speed of wound healing, and enhance the effect of wound repair is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] To address the shortcomings of the existing technology, the present invention aims to effectively promote the rapid repair of wound cells, increase the speed of wound healing, and improve the wound repair effect. The present invention provides a wound repair gel, the preparation materials of which include: compound amino acids, glucose, polyvinyl alcohol, and insulin added in a weight ratio; and polyvinylpyrrolidone and gelling additives added in a volume ratio.

[0006] According to a first embodiment of the present invention, a wound repair gel is provided:

[0007] A wound repair gel, the materials for which the repair gel is prepared include: compound amino acids, glucose, polyvinyl alcohol, and insulin added in weight ratio; and polyvinylpyrrolidone and gel additives added in volume ratio.

[0008] Furthermore, as a more preferred embodiment of the present invention, the materials for preparing the repair gel further include hydrogen peroxide added in a volume ratio.

[0009] Furthermore, as a more preferred embodiment of the present invention, the materials for preparing the repair gel further include: sodium benzoate and gentamicin added in proportion by weight.

[0010] Furthermore, as a more preferred embodiment of the present invention, the gel additive is prepared by dissolving 10-20g of carbomer and 10-20g of triethanolamine in purified water.

[0011] Furthermore, as a more preferred embodiment of the present invention, the materials for preparing the repair gel further include: sodium chloride, calcium chloride, and potassium chloride added in a weight ratio.

[0012] Furthermore, as a more preferred embodiment of the present invention, the materials for preparing the repair gel further include: dextran, glycerol, and propylene glycol added in weight ratio.

[0013] Further, as a more preferred embodiment of the present invention, its preparation method is as follows: 0.6-0.8g of the composite amino acid, 3-5g of glucose, 3-5g of sodium chloride, 0.1-0.3g of calcium chloride, and 0.2-0.4g of potassium chloride are dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone according to the following weight ratio to obtain 100ml of the first nutrient solution; 0.5-2g of polyvinyl alcohol, 0.1-1ml of insulin, 1-4g of dextran, and 8g of glycerol are added according to the following weight ratio. -20g of propylene glycol and 3-10g of propylene glycol are dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the second group of cell proliferation and immune solutions; 30-20ml of 30% hydrogen peroxide solution is dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone according to the volume ratio to obtain 100ml of the third group of oxygenation solutions; 2-10g of sodium benzoate and 0.5-3mg of gentamicin are dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone according to the weight ratio to obtain 100ml of the fourth group of anti-infection solutions.

[0014] Furthermore, in a more preferred embodiment of the present invention, the glucose is D-glucan; and the dextran is β-glucan.

[0015] Furthermore, in a more preferred embodiment of the present invention, the composite amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:9.

[0016] According to a second embodiment of the present invention, a method for preparing a wound repair gel is provided:

[0017] A method for preparing a wound repair gel, the method comprising the following steps:

[0018] S1. Preparation of nutrient system: Dissolve 0.6-0.8g of compound amino acids, 3-5g of D-glucose, 3-5g of sodium chloride, 0.1-0.3g of calcium chloride, and 0.2-0.4g of potassium chloride in 0.1-1.0% polyvinylpyrrolidone aqueous solution to obtain 100ml of the first nutrient solution.

[0019] S2. Preparation of cell proliferation component system: Dissolve 0.5-2g of polyvinyl alcohol, 0.1-1ml of insulin, 1-4g of dextran, 8-20g of glycerol, and 3-10g of propylene glycol in 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the second group of cell proliferation and immune solution.

[0020] S3. Preparation of blood cell oxygenation component system: Dissolve 30-20 ml of 30% hydrogen peroxide solution in 0.1-1.0% polyvinylpyrrolidone aqueous solution, mix well to obtain 100 ml of the third group of oxygenation solution;

[0021] S4. Preparation of anti-infective component system: Dissolve 2-10g of sodium benzoate and 0.5-3mg of gentamicin in 0.1-1.0% aqueous solution of polyvinylpyrrolidone, and obtain 100ml of the fourth anti-infective solution.

[0022] S5. Preparation of gel component system: Disperse 10-20g of carbomer in 800-900g of purified water and let stand overnight until the carbomer is fully dispersed and uniform, to obtain a carbomer solution that has been left to stand overnight; Dissolve 10-20g of triethanolamine in 70-170g of purified water, mix well, and slowly pour into the carbomer solution that has been left to stand overnight, and stir well to obtain the fifth group of gel additives.

[0023] S6. Preparation of wound repair gel: Take the first group of nutrient solution, the second group of cell proliferation and immune solution, the third group of oxygenation solution, the fourth group of anti-infection solution and the fifth group of gel additive in a volume ratio of 1:1:1:1:6 and mix them evenly to obtain the wound repair gel.

[0024] Furthermore, in a more preferred embodiment of the present invention, the glucose is D-glucan; and the dextran is β-glucan.

[0025] Furthermore, in a more preferred embodiment of the present invention, the composite amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:9.

[0026] It should be noted that a method for preparing a wound repair gel further includes the following steps:

[0027] During the preparation of S601, the first group of nutrient solutions, the second group of cell proliferation and immune solutions, the third group of oxygenation solutions, the fourth group of anti-infection solutions, and the fifth group of gel additives are stored at low temperatures to improve the quality of the final wound repair adhesive.

[0028] It should be noted that a method for preparing a wound repair gel further includes the following steps:

[0029] During the mixing of the first group of nutrient solutions, the second group of cell proliferation and immune solutions, the third group of oxygenation solutions, the fourth group of anti-infection solutions, and the fifth group of gel additives in S602, the mixture needs to be carried out at a solution temperature of 20-40℃ to increase the mixing speed and improve production efficiency.

[0030] It should be noted that a method for preparing a wound repair gel further includes the following steps:

[0031] S301 introduces ozone gas into the third group of oxygenated solutions to increase the overall oxidizing power of the third group of oxygenated solutions.

[0032] According to a third embodiment of the present invention, a wound autologous blood repair gel is provided:

[0033] An autologous blood wound repair gel, the materials for preparing the autologous blood wound repair gel include: an equal volume of the wound repair gel and the patient's autologous venous blood.

[0034] According to a fourth embodiment of the present invention, a method for preparing autologous blood repair gel for wounds is provided:

[0035] A method for preparing autologous blood repair gel for wounds, the method comprising: S7, preparing autologous blood repair gel for wounds: mixing an equal volume of venous blood from a wound patient with an equal volume of wound repair gel to obtain autologous blood repair gel for wounds.

[0036] Furthermore, addressing the shortcomings of existing PRP (Platelet-Rich Plasma) techniques—complex procedures, limited components, high costs, and the tendency for direct application of autologous whole blood to the wound surface to promote bacterial growth—this invention aims to provide an autologous blood gel for the treatment of chronic, difficult-to-heal wounds. The preparation method of this autologous blood gel is simple, its application convenient, and it exhibits highly effective wound healing promotion, broad-spectrum antibacterial properties, and good wound protection. This autologous blood gel comprises a five-component system: a cell nutrition system consisting of up to eighteen amino acids and autologous blood; a cell proliferation and immune regulation system consisting of polyvinyl alcohol, insulin, and beta-glucan; an oxygenation system provided by low-dose hydrogen peroxide and an anti-infection system consisting of benzoic acid and gentamicin; and a gel system consisting of carbomer and triethanolamine. This autologous blood repair gel for wounds is fully functional and designed specifically for the pathophysiological characteristics of wound healing. The components of the autologous blood gel are dissolved separately in polyvinylpyrrolidone (PVP) and then mixed with the carbomer gel components. This reduces the potential antagonism between the components and allows for the slow release of the active ingredients, thus reducing potential side effects.

[0037] Compared with existing technologies, the technical solution provided in this application constructs a basic nutrient and energy system for cells by mixing compound amino acids, glucose, and polyvinylpyrrolidone (PVP), and constructs a cell proliferation and immune system by mixing polyvinyl alcohol (PVA), insulin, and PPVP. Finally, a wound repair gel is obtained by mixing a PPVP solution containing compound amino acids, glucose, PVA, and insulin with a gelling additive. This wound repair gel can provide the cells in the wound with the nutrients needed for repair and stimulate the self-repair of wound cells. The technical solution provided in this application can effectively promote the rapid repair of wound cells, improve the speed of wound healing, and enhance the wound repair effect. Attached Figure Description

[0038] Figure 1 This is a flowchart illustrating the preparation method of the wound repair gel in an embodiment of the present invention;

[0039] Figure 2 This is a flowchart illustrating the method for preparing autologous blood repair gel for wounds in an embodiment of the present invention;

[0040] Figure 3This is a comparative experimental diagram showing the addition of different concentrations of hydrogen peroxide to venous blood in an embodiment of the present invention.

[0041] As shown in the figure, the top three groups are the control groups A, B, and C, which contain different doses of hydrogen peroxide. After oxygenation, they turn into a bright red color similar to arterial blood. D is the patient's venous blood, which is dark red. The blood with high oxygenation provides local oxygen supply to the wound, which is beneficial for wound repair.

[0042] Figure 4 This is a schematic diagram of the wound condition before treatment with autologous blood repair gel in Experimental Example 2 of the present invention;

[0043] Figure 5 This is a schematic diagram of the wound condition after treatment with autologous blood repair gel in Experimental Example 2 of the present invention. Detailed Implementation

[0044] To enable those skilled in the art to better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0045] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly set on the other component; when a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to the other component.

[0046] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0047] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" or "several" means two or more, unless otherwise explicitly specified.

[0048] It should be noted that the structures, proportions, sizes, etc., shown in the accompanying drawings of this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed in the specification, and are not intended to limit the conditions under which this application can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size should still fall within the scope of the technical content disclosed in this application, provided that they do not affect the effects and purposes that this application can produce.

[0049] According to a first embodiment of the present invention, a wound repair gel is provided:

[0050] A wound repair gel, the materials for which the repair gel is prepared include: compound amino acids, glucose, polyvinyl alcohol, and insulin added in weight ratio; and polyvinylpyrrolidone and gel additives added in volume ratio.

[0051] This application provides a technical solution for a wound repair gel. This solution constructs a basic cellular nutrition and energy system by mixing compound amino acids, glucose, and polyvinylpyrrolidone (PVP), and a cell proliferation and immune system by mixing polyvinyl alcohol (PVA), insulin, and PPVP. Finally, the wound repair gel is obtained by mixing a PPVP solution containing compound amino acids, glucose, PVA, and insulin with a gel additive. This wound repair gel provides the necessary nutrients for wound cell repair and stimulates the self-repair of wound cells. The technical solution provided by this application can effectively promote rapid wound cell repair, increase the speed of wound healing, and improve the wound repair effect.

[0052] Specifically, in this embodiment of the invention, the materials used to prepare the repair gel also include hydrogen peroxide added in a volume ratio.

[0053] It should be noted that, in this embodiment, by adding hydrogen peroxide to the wound repair gel, sufficient oxygen can be provided to the wound, promoting the oxygen required for the wound cell repair process, that is, promoting the effects of polyvinyl alcohol, insulin, and dextran on cell proliferation and repair.

[0054] Specifically, in this embodiment of the invention, the materials used to prepare the repair gel also include sodium benzoate and gentamicin added in proportion by weight.

[0055] By adding sodium benzoate and gentamicin to the wound repair gel, the gel gains anti-infection capabilities, thereby enhancing the wound's resistance to infection and promoting rapid wound healing.

[0056] Specifically, in this embodiment of the invention, the gel additive is prepared by dissolving 10-20g of carbomer and 10-20g of triethanolamine in purified water.

[0057] It should be noted that the gel additive is prepared by mixing carbomer and triethanolamine with purified water, a commonly used method for gel additives. In this embodiment, carbomer is a high molecular weight polymer that promotes the uniform distribution of the first group of nutrient solutions, the second group of cell proliferation and immune solutions, the third group of oxygenation solutions, and the fourth group of anti-infection solutions, thereby achieving a slow release of the medication onto the wound.

[0058] Specifically, in this embodiment of the invention, the materials used to prepare the repair gel also include sodium chloride, calcium chloride, and potassium chloride added in a weight ratio.

[0059] It should be noted that supplementing with sodium chloride, calcium chloride, and potassium chloride can replenish the basic inorganic nutrients needed by human cells from the outside, promoting wound repair.

[0060] Specifically, in this embodiment of the invention, the materials used to prepare the repair gel also include: dextran, glycerol, and propylene glycol added in proportion by weight.

[0061] It should be noted that dextran is a polysaccharide. During blood transfusion, dextran can replace a portion of whole blood as a plasma volume expander, thereby promoting the binding of wound repair gel to the capillaries of the wound and facilitating the entry of the drug components of the wound repair gel into the wound.

[0062] Specifically, in this embodiment of the invention, the preparation method is as follows: 0.6-0.8g of the composite amino acid, 3-5g of glucose, 3-5g of sodium chloride, 0.1-0.3g of calcium chloride, and 0.2-0.6g of potassium chloride are dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the first nutrient solution; 0.5-2g of polyvinyl alcohol, 0.1-1ml of insulin, 1-4g of dextran, and 8-2g of glycerol are added according to the weight ratio. 0g of propylene glycol and 3-10g of the propylene glycol solution were dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the second group of cell proliferation and immune solutions; 3-20ml of 30% hydrogen peroxide solution was dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the third group of oxygenation solutions; 2-10g of sodium benzoate and 0.5-3mg of gentamicin were dissolved in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the fourth group of anti-infection solutions.

[0063] It should be noted that polyvinylpyrrolidone (PVP) has excellent physiological inertness, does not participate in human metabolism, and has excellent biocompatibility, causing no irritation to the skin, mucous membranes, or eyes. Therefore, the wound repair gel provided in this application, using PPVP as an excipient, can effectively promote the binding of the nutritional and repair components within the wound repair gel to the wound surface, thereby promoting rapid wound healing.

[0064] Specifically, in this embodiment of the invention, the glucose is D-glucan and the dextran is β-glucan.

[0065] Specifically, in this embodiment of the invention, the composite amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:9.

[0066] According to a second embodiment of the present invention, a method for preparing a wound repair gel is provided:

[0067] A method for preparing a wound repair gel, the method comprising the following steps:

[0068] S1. Preparation of nutrient system: Dissolve 0.6-0.8g of compound amino acids, 3-5g of D-glucose, 3-5g of sodium chloride, 0.1-0.3g of calcium chloride, and 0.2-0.6g of potassium chloride in 0.1-1.0% polyvinylpyrrolidone aqueous solution to obtain 100ml of the first nutrient solution.

[0069] S2. Preparation of cell proliferation component system: Dissolve 0.5-2g of polyvinyl alcohol, 0.1-1ml of insulin, 1-4g of dextran, 8-20g of glycerol, and 3-10g of propylene glycol in 0.1-1.0% aqueous solution of polyvinylpyrrolidone to obtain 100ml of the second group of cell proliferation and immune solution.

[0070] S3. Preparation of blood cell oxygenation component system: Dissolve 3-20 ml of 30% hydrogen peroxide solution in 0.1-1.0% polyvinylpyrrolidone aqueous solution, mix well to obtain 100 ml of the third group of oxygenation solution;

[0071] S4. Preparation of anti-infective component system: Dissolve 2-10g of sodium benzoate and 0.5-3mg of gentamicin in 0.1-1.0% aqueous solution of polyvinylpyrrolidone, and obtain 100ml of the fourth anti-infective solution.

[0072] S5. Preparation of gel component system: Disperse 10-20g of carbomer in 800-900g of purified water and let stand overnight until the carbomer is fully dispersed and uniform, to obtain a carbomer solution that has been left to stand overnight; Dissolve 10-20g of triethanolamine in 70-170g of purified water, mix well, and slowly pour into the carbomer solution that has been left to stand overnight, and stir well to obtain the fifth group of gel additives.

[0073] S6. Preparation of wound repair gel: Take the first group of nutrient solution, the second group of cell proliferation and immune solution, the third group of oxygenation solution, the fourth group of anti-infection solution and the fifth group of gel additive in a volume ratio of 1:1:1:1:6 and mix them evenly to obtain the wound repair gel.

[0074] This application also provides a method for preparing a wound repair gel. The method involves dissolving the first component (compound amino acids, glucose, sodium chloride, calcium chloride, potassium chloride), the second component (polyvinyl alcohol, insulin, dextran, glycerol, propylene glycol), the third component (hydrogen peroxide solution), and the fourth component (sodium benzoate, gentamicin) in equal volumes of polyvinylpyrrolidone (PVP) solution. Then, the resulting first group of nutrient solutions, the second group of cell proliferation and immune solutions, the third group of oxygenation solutions, and the fourth group of anti-infection solutions are mixed with a gel additive to obtain the wound repair gel. The wound repair gel prepared using this method can effectively promote the rapid repair of wound cells, increase the speed of wound healing, and improve the wound repair effect.

[0075] Specifically, in this embodiment of the invention, the glucose is D-glucan and the dextran is β-glucan.

[0076] Specifically, in this embodiment of the invention, the composite amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:9.

[0077] According to a third embodiment of the present invention, a wound autologous blood repair gel is provided:

[0078] An autologous blood wound repair gel, the materials for preparing the autologous blood wound repair gel comprising: an equal volume of the wound repair gel described in the first embodiment and patient autologous venous blood.

[0079] It should be noted that recent studies have shown that autologous whole blood has a good effect on the treatment of chronic wounds. This involves drawing a small amount of blood from the patient's venous blood and applying it evenly to the wound, which can accelerate wound healing, likely due to the presence of various growth factors in the blood. Autologous whole blood is known to contain growth factors, red blood cells, white blood cells, platelets, serum, fibrin, and other substances. Kushnir et al. believe that in the early stages of wound repair, blood clots can rebuild hemostasis and protect the wound through their fibrin scaffold. This fibrin scaffold, as a temporary matrix, can promote angiogenesis and, by carrying related cells and factors, promote healing and reduce inflammation. Domestic scholars Cai Qiuni and Liu Xiuqing et al., in a clinical study, drew venous blood from diabetic patients and applied it directly to the wounds of diabetic foot patients, achieving good therapeutic results. However, applying autologous whole blood directly to the surface of difficult-to-heal wounds deprives blood cells of nutritional support, resulting in a shorter survival time and increasing the risk of bacterial growth and infection.

[0080] According to a fourth embodiment of the present invention, a method for preparing autologous blood repair gel for wounds is provided:

[0081] A method for preparing autologous blood repair gel for wounds, the method comprising: S7, preparing autologous blood repair gel for wounds: mixing an equal volume of venous blood from a wound patient with an equal volume of wound repair gel to obtain autologous blood repair gel for wounds.

[0082] Example 1

[0083] A wound repair gel, the materials for which the repair gel is prepared include: compound amino acids, glucose, polyvinyl alcohol, and insulin added in weight ratio; and polyvinylpyrrolidone and gel additives added in volume ratio.

[0084] Example 2

[0085] Repeat Example 1, except that the materials used to prepare the repair gel also include hydrogen peroxide added in a volume ratio.

[0086] Example 3

[0087] Example 2 was repeated, except that the materials used to prepare the repair gel also included sodium benzoate and gentamicin added in proportion by weight.

[0088] Example 4

[0089] Example 3 was repeated, except that the gel additive was prepared by dissolving 15g of carbomer and 15g of triethanolamine in 970g of purified water.

[0090] Example 5

[0091] Example 4 is repeated, except that the materials used to prepare the repair gel also include sodium chloride, calcium chloride, and potassium chloride added in proportion by weight.

[0092] Example 6

[0093] Example 5 was repeated, except that the materials used to prepare the repair gel also included dextran, glycerol, and propylene glycol added in the indicated weight ratios.

[0094] Example 7

[0095] Example 6 was repeated, except that its preparation method was as follows: 657 mg of the composite amino acid, 4.5 g of glucose, 4.5 g of sodium chloride, 0.2 g of calcium chloride, and 0.4 g of potassium chloride were dissolved in a 1% polyvinylpyrrolidone aqueous solution according to the weight ratio to obtain 100 ml of the first group of nutrient solutions; 1 g of polyvinyl alcohol, 50 units of insulin, 2 g of dextran, 10 g of glycerol, and 5 g of propylene glycol were dissolved in a 1% polyvinylpyrrolidone aqueous solution according to the weight ratio to obtain 100 ml of the second group of cell proliferation and immune solutions; 10 ml of 30% hydrogen peroxide solution was dissolved in a 1% polyvinylpyrrolidone aqueous solution according to the volume ratio to obtain 100 ml of the third group of oxygenation solutions; and 5 g of sodium benzoate and 1.5 mg of gentamicin were dissolved in a 1% polyvinylpyrrolidone aqueous solution according to the weight ratio to obtain 100 ml of the fourth group of anti-infection solutions.

[0096] Example 8

[0097] Example 7 is repeated, except that the glucose is D-glucan and the dextran is β-glucan. The complex amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:9.

[0098] Example 9

[0099] A method for preparing a wound repair gel, the method comprising the following steps:

[0100] S1. Preparation of nutrient system: Dissolve 657mg of compound amino acids, 4.5g of glucose, 4.5g of sodium chloride, 0.2g of calcium chloride, and 0.4g of potassium chloride in a 1% polyvinylpyrrolidone aqueous solution to obtain 100ml of the first nutrient solution.

[0101] S2. Preparation of cell proliferation component system: Dissolve 1g of polyvinyl alcohol, 50 units of insulin, 2g of dextran, 10g of glycerol, and 5g of propylene glycol in a 1% polyvinylpyrrolidone aqueous solution to obtain 100ml of the second group of cell proliferation and immune solution.

[0102] S3. Preparation of blood cell oxygenation component system: Dissolve 10 ml of 30% hydrogen peroxide solution in 1% polyvinylpyrrolidone aqueous solution and mix well to obtain 100 ml of the third group of oxygenation solution.

[0103] S4. Preparation of anti-infective component system: Dissolve 5g of sodium benzoate and 1.5mg of gentamicin in 1% polyvinylpyrrolidone aqueous solution and fully dissolve to obtain 100ml of the fourth group of anti-infective solution;

[0104] S5. Preparation of gel component system: Disperse 15g of carbomer in 850g of purified water and let stand overnight until the carbomer is fully dispersed and uniform, to obtain a carbomer solution that has been left to stand overnight; Dissolve 15g of triethanolamine in 120g of purified water, mix well, and slowly pour into the carbomer solution that has been left to stand overnight, and stir well to obtain the fifth group of gel additives.

[0105] S6. Preparation of wound repair gel: Take the first group of nutrient solution, the second group of cell proliferation and immune solution, the third group of oxygenation solution, the fourth group of anti-infection solution and the fifth group of gel additive in a volume ratio of 1:1:1:1:6 and mix them evenly to obtain the wound repair gel.

[0106] Example 10

[0107] Example 9 was repeated, except that the glucose was D-glucan and the dextran was β-glucan. The complex amino acids included: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine was 1:2:4:6:6:7:7:9.

[0108] Example 11

[0109] An autologous blood wound repair gel, the materials for preparing the autologous blood wound repair gel include: an equal volume of the wound repair gel and the patient's autologous venous blood.

[0110] Example 12

[0111] A method for preparing autologous blood repair gel for wounds, the method comprising: S7, preparing autologous blood repair gel for wounds: mixing an equal volume of venous blood from a wound patient with an equal volume of wound repair gel to obtain autologous blood repair gel for wounds.

[0112] Experimental Example 1:

[0113] As attached Figure 3 By mixing venous blood from wound patients with an equal volume of autologous blood repair gel, the venous blood instantly becomes fully oxygenated arterial blood. The autologous blood repair gel then covers the wound area, improving wound hypoxia and promoting healing. In a sample of venous blood with added hydrogen peroxide, three concentrations of hydrogen peroxide were mixed with equal volumes of venous blood:

[0114]

[0115] As shown in the image, venous blood with an added 0.3% hydrogen peroxide solution has a similar appearance to arterial blood. Hydrogen peroxide can help improve the oxygen content of the patient's venous blood in the autologous blood repair gel, enhance blood activity, and promote rapid wound healing.

[0116] Experimental Example 2:

[0117] Experimental study on the effect of promoting wound healing. Twenty eligible hospitalized diabetic patients with chronic skin wounds were selected. Patients were randomly divided into a control group and an experimental group (n=10 in each group) using a random number table. The control group received gentle debridement and foam dressing, while the experimental group received autologous blood repair gel. This study was approved by the ethics committee of a tertiary hospital, and all patients signed informed consent forms. The results of diabetic foot wound healing are shown in the table below. Figures 4-5 Experimental results show that autologous blood repair gel can effectively promote the healing of diabetic foot wounds.

[0118]

[0119] Table 1 Comparison of wound healing rates before and after treatment in the two groups of patients (x±s)

[0120] Experimental Example 3:

[0121] Experimental study on the effect of promoting capillary proliferation in wounds. Following Experiment Example 2, capillary counts were performed on two groups of patients treated with autologous blood repair gel on days 0 and 15. Granulation tissue from the center of the wound was collected from both groups on days 0 and 15 of treatment, placed in 10% formaldehyde solution, and stored at 4°C. The tissue was then embedded, sectioned, and observed under a microscope. The five areas with the most capillaries under low magnification were identified, and capillary counts were performed under high magnification (400×). The average value under high magnification was taken as the capillary count for that section. The autologous blood repair gel group showed a significant increase in capillary proliferation in the wound, as shown in Table 2.

[0122]

[0123] Table 2 Comparison of capillary counts between the two groups of patients before and after treatment (x±s)

[0124] Experimental Example 4:

[0125] Antibacterial efficacy experiment of autologous blood wound repair gel. According to Appendix C of the "Hygienic Standard for Disposable Sanitary Products" GB15979-2002, 5 mL of sample was added to three separate test tubes. 100 μL of bacterial suspensions of Staphylococcus aureus, Escherichia coli, and Candida albicans were mixed with the sample in each tube. Timing was started, and after 2 min, 5 min, 10 min, and 20 min, 0.5 mL of the mixture was added to a test tube containing 4.5 mL of PBS and mixed thoroughly. 0.5 mL of each mixture was then placed in two petri dishes, and 15 mL of nutrient agar or Sabouraud broth cooled to 40–45 °C was poured in. The dishes were shaken to mix thoroughly, and after solidification, they were inverted and incubated at 35 °C for 48 hours. Colony counting was then performed. The inhibition rate was calculated compared with the blank control group. The experimental results showed that the autologous blood wound repair gel had a 100% inhibition rate against Staphylococcus aureus, Escherichia coli, and Candida albicans.

[0126] Experimental Example 5:

[0127] In vitro cytotoxicity assay. Following the in vitro cytotoxicity test standard GB / T 16886.5-2017, nine culture dishes containing pre-cultured L-929 monolayer cells were prepared. The original culture medium was discarded, and 0.8 mL of fresh medium was added. Positive controls, negative controls, and the test sample were added to each dish, and the cells were cultured at 37℃ and 5% CO2 for 24 hours. After 24 hours of culture, three dishes from each group were collected for microscopic observation of cell morphology and measurement of the toxic area. Under the experimental conditions, the autologous blood repair gel on the wound showed no toxicity to L-929 cells, with a cell reaction grade of 0.

[0128] Experimental Example 6:

[0129] Skin irritation test. According to GB / T 16886.10-2005, three healthy rabbits were used. The rabbits were shaved 24 hours before the test to avoid skin damage. The prepared test sample was directly applied to one side of the rabbit's back at the test site. A blank control (0.9% sodium chloride injection) was applied to the other side using the same method, and the application was immediately fixed. The dressing was removed 4 hours after fixation, and the application and test sites were marked. Application was repeated for 14 consecutive days. At 1 hour, 24 hours, 48 ​​hours, and 72 hours after dressing removal, the reaction of the test site and its skin tissue, including erythema, edema, and necrosis, was observed. After 72 hours of observation, the test sample and control were applied daily using the same method. The condition of the contact site was recorded 1 hour after dressing removal and before re-exposure. After the last exposure, the condition of each contact site was recorded at 1 hour, 24 hours, 48 ​​hours, and 72 hours after dressing removal. The occurrence of erythema and edema can be scored on a scale of 0, 1, 2, 3, or 4. Under the experimental conditions, the cumulative stimulation index of the autologous blood wound repair gel on rabbits in multiple skin stimulation experiments was 0, indicating a very mild reaction.

[0130] Experimental Example 7:

[0131] 2) Skin irritation test: According to GB / T 16886.10-2005, three healthy rabbits were used. The rabbits were shaved 24 hours before the test to avoid skin damage. The prepared test sample was directly applied to one side of the rabbit's back at the test site. A blank control (0.9% sodium chloride injection) was applied to the other side using the same method and immediately fixed. The patch was removed 4 hours after fixation, and the test site was marked. The application was repeated for 14 consecutive days. After removing the patch, the reaction of the test site and skin tissue, including erythema, edema, and necrosis, was observed at 1 hour, 24 hours, 48 ​​hours, and 72 hours. After 72 hours of observation, the test sample and control were applied daily using the same method. The condition of the contact site was recorded 1 hour after patch removal and before re-exposure. After the last exposure, the condition of each contact site was recorded at 1 hour, 24 hours, 48 ​​hours, and 72 hours after patch removal. The occurrence of erythema and edema can be scored on a scale of 0, 1, 2, 3, or 4. Under the experimental conditions, the cumulative stimulation index of the autologous blood wound repair gel on rabbits in multiple skin stimulation experiments was 0, indicating a very mild reaction.

[0132] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A wound repair gel, characterized in that, The materials used to prepare the repair gel include: compound amino acids, glucose, polyvinyl alcohol, and insulin added in weight ratio; and polyvinylpyrrolidone and gel additives added in volume ratio. The materials used to prepare the repair gel also include: hydrogen peroxide added in volume proportions; sodium benzoate and gentamicin added in weight proportions; The gel additive is prepared by dissolving 10-20g of carbomer and 10-20g of triethanolamine in purified water. The glucose is D-glucose; the complex amino acids include: tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine; the weight ratio of tryptophan, methionine, phenylalanine, threonine, valine, isoleucine, leucine, and lysine is 1:2:4:6:6:7:7:

9.

2. The wound repair gel according to claim 1, characterized in that, The materials used in preparing the repair gel also include: sodium chloride, calcium chloride, and potassium chloride added in a weight ratio; and / or the materials used in preparing the repair gel also include: dextran, glycerol, and propylene glycol added in a weight ratio; wherein the dextran is β-glucan.

3. A method for preparing the wound repair gel as described in claim 2, characterized in that, Includes the following steps: S1. Preparation of the nutrient system: Dissolve 0.6-0.8g of compound amino acids, 3-5g of D-glucose, 3-5g of sodium chloride, 0.1-0.3g of calcium chloride, and 0.2-0.6g of potassium chloride in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone, and fully dissolve to obtain 100ml of the first nutrient solution; S2. Preparation of the cell proliferation component system: Dissolve 0.5-2g of polyvinyl alcohol, 0.1-1ml of insulin, 1-4g of dextran, 8-20g of glycerol, and 3-10g of propylene glycol in a 0.1-1.0% aqueous solution of polyvinylpyrrolidone, and fully dissolve to obtain 100ml of the second cell proliferation and immune solution; S3. Preparation of the blood cell oxygenation component system: Take 30% peroxide... S4. Prepare the anti-infective component system: Dissolve 2-10g of sodium benzoate and 0.5-3mg of gentamicin in 0.1-1.0% aqueous solution of polyvinylpyrrolidone, and mix thoroughly to obtain 100ml of the third group of oxygenation solution; S5. Prepare the gel component system: Disperse 10-20g of carbomer in 800-900g of purified water, let stand overnight, and obtain a carbomer solution that has been left to stand overnight; Dissolve 10-20g of triethanolamine in 70-170g of purified water, mix thoroughly, and slowly pour into the carbomer solution that has been left to stand overnight, and stir thoroughly to obtain the fifth group of gel additives; S6. Preparation of wound repair gel: Take the first group of nutrient solution, the second group of cell proliferation and immune solution, the third group of oxygenation solution, the fourth group of anti-infection solution and the fifth group of gel additive in a volume ratio of 1:1:1:1:6 and mix them evenly to obtain the wound repair gel.

4. A wound autologous blood repair gel based on the wound repair gel according to any one of claims 1-2, characterized in that, The materials used to prepare the autologous blood repair gel include: an equal volume of the wound repair gel described in any one of claims 1-2 and the patient's autologous venous blood.

5. A method for preparing autologous blood wound repair gel based on the wound repair gel preparation method of claim 3, characterized in that, The method for preparing the autologous blood repair gel includes: S7, preparing the autologous blood repair gel for the wound: mixing an equal volume of venous blood from the patient with an equal volume of wound repair gel to obtain the autologous blood repair gel for the wound.

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