Low-cost air pollen online observation device and observation method with high precision

Abstract

The application discloses a low-cost air pollen online observation device with high precision and an observation method, which comprises a shell, wherein an aerosol particle collecting device for collecting aerosol particles containing pollen in air is arranged on the shell; a rotating platform assembly for bearing and conveying the aerosol particles is arranged below the aerosol particle collecting device in the shell; a wrapping assembly for wrapping the aerosol particles and an aggregation assembly for aggregating the wrapped aerosol particles are arranged on the rotating platform assembly; an imaging acquisition assembly for observing the wrapped and aggregated aerosol particle samples is arranged above one side of the rotating platform and can adjust the observation angle; and the aerosol particle collecting device, the rotating platform assembly, the wrapping assembly and the imaging acquisition assembly are connected with a logic controller. The application can greatly reduce the loss of manpower and material resources, improve the observation efficiency and observation accuracy, and further lay a foundation for the construction of a pollen observation network.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of online observation of airborne pollen, and in particular to a low-cost airborne pollen online observation device with high precision and an observation method. BACKGROUND

[0002] Plants bring us many benefits, but they also produce allergenic pollen, which has a real impact on human health. This phenomenon is further exacerbated by climate warming and increased carbon emissions, not only causing respiratory diseases, but also causing digestive and reproductive system problems.

[0003] The proportion of people affected by airborne pollen in the world is relatively high, among which more than 24% of Europeans, more than 20% of Americans and 10-20% of Japanese are affected by allergenic pollen, and the proportion of children is as high as 40%. The proportion of people affected in China is about 5-10%. With the continuous advancement of urbanization in China, the concentration of population will continuously increase the proportion of people affected in China. The incidence of pollen allergy in various parts of the world is increasing year by year, and the incidence in China is 0.5%-1%, and in high-incidence areas it can reach 5%. With the influence of global warming, rising temperatures and carbon dioxide concentrations will encourage plants to release more pollen, leading to an increase in pollen concentration in the air and an extension of the duration of the plant flowering period. In addition, the increase in carbon dioxide concentration will also increase the allergenic peptides on the pollen. As we all know, allergenic peptides can trigger the human immune system and increase the risk of human allergy. Therefore, with global warming, pollen allergy diseases will face unprecedented complex challenges.

[0004] Developing an automatic pollen observation device to conduct fine observation of pollen can provide fine prediction data support for pollen concentration, and at the same time guide sensitive groups to arrange medication reasonably and reduce pollen allergy symptoms, and contribute to the development of the "Healthy China" strategy. In addition, garden plants play an important role in purifying air and water, maintaining water and soil, improving urban microclimate, and reducing urban noise, but they are also one of the important factors affecting the types and concentrations of urban air pollen. The observation results of pollen can provide suggestions for reasonable urban garden planning and promote the construction of China's urban ecological environment.

[0005] To study the impact of pollen on humans, the first problem to be solved is pollen monitoring. Globally, pollen identification and counting is still mainly done by hand. However, due to the high technical requirements of this work, and the difficulty of updating data in real time, it has become a bottleneck for subsequent research. Developing an automatic airborne pollen observation system has great scientific value.

[0006] At present, the gravity sedimentation method is generally used for daily pollen observation in China, which needs to consume a large amount of manpower and material resources and is not suitable for large-scale network construction. On the other hand, the temporal and spatial resolution cannot meet the current air pollen service demand, which seriously hinders the research and data application of air pollen in China. In Europe, Japan and the United States, the air humidity is higher than that in China due to the influence of marine climate, and the pollen types and variation rules are significantly different from those in China. When using foreign automatic pollen observation equipment, the differences in climate of different regions need to be avoided first. Secondly, the equipment is expensive, the network monitoring cost is difficult to bear, the stability of the equipment is unknown, the maintenance cost is difficult to estimate, and there is a risk of "neck block". Therefore, it is necessary to develop an online automatic air pollen observation equipment. The successful development of the equipment not only fills the blank of automatic pollen observation field with independent intellectual property rights in China, but also reduces the work intensity of pollen observation personnel, greatly reduces the loss of manpower and material resources, improves the observation efficiency and observation accuracy, and further lays a foundation for the construction of pollen observation network. SUMMARY

[0007] The technical problem to be solved by the present application is to provide a low-cost air pollen online observation equipment with high precision, which can not only reduce the work intensity of pollen observation personnel, greatly reduce the loss of manpower and material resources, but also improve the observation efficiency and observation accuracy, and further lay a foundation for the construction of pollen observation network.

[0008] To solve the above technical problems, the technical solutions adopted by the present application are as follows.

[0009] The low-cost air pollen online observation equipment with high precision comprises a shell, wherein an aerosol particle collecting device for collecting aerosol particles containing pollen in the air is arranged on the shell; a rotating platform assembly for carrying and conveying the aerosol particles collected by the aerosol particle collecting device is arranged below the aerosol particle collecting device in the shell; a wrapping assembly for wrapping the aerosol particles and an aggregation assembly for aggregating the wrapped aerosol particles are arranged on the rotating platform assembly; an imaging acquisition assembly for observing the sample of the wrapped and aggregated aerosol particles is arranged above one side of the rotating platform; and a logic controller for automatically controlling the online observation of air pollen is connected to the aerosol particle collecting device, the rotating platform assembly, the wrapping assembly and the imaging acquisition assembly.

[0010] Preferably, the imaging acquisition assembly comprises an imaging acquisition device and an adjusting device for adjusting the observation angle of the imaging acquisition device.

[0011] The adjusting device includes an adjusting device stepper motor and an X / Y / Z axis precision displacement platform, the adjusting device stepper motor is arranged on the side of the rotating platform assembly and the rotating shaft is arranged upward, and the controlled end of the adjusting device stepper motor is connected with the output end of the logic controller; the X / Y / Z axis precision displacement platform is arranged above the side of the rotating platform assembly through a transmission component connected with the rotating shaft of the adjusting device stepper motor, and a lens barrel clamp located above the rotating platform is arranged on the X / Y / Z axis precision displacement platform;

[0012] The imaging acquisition assembly includes a microscope, an LED light source and an image sensor, the microscope includes an objective lens and a lens barrel connected with the objective lens through an objective lens clamp, the lens barrel is longitudinally clamped on the lens barrel clamp, and the objective lens is arranged toward the rotating platform assembly; the LED light source is arranged on the bottom shell plate of the shell and faces the objective lens, the controlled end of the LED light source is connected with the output end of the logic controller, and an LED lens for condensing light is buckled on the LED light source; the image sensor is arranged on the top of the lens barrel through an image sensor support arranged on the upper part of the lens barrel, and the output end of the image sensor is connected with the input end of the logic controller; the LED lens, the objective lens and the image sensor are on the same vertical line.

[0013] Preferably, the aerosol particle collection device is a cyclone cutter longitudinally penetrating the upper shell plate of the shell, the cyclone cutter includes a cyclone cutter air inlet part fixedly connected with the upper shell plate of the shell and exposed outside the shell and a cyclone cutter gas quality separation part located inside the shell and combinedly connected with the cyclone cutter air inlet part; three main air inlets and three airflow exciting air inlets are arranged around the top of the cyclone cutter air inlet part at an interval of 120°, the main air inlets and the airflow exciting air inlets are alternately arranged in a circumferential direction; an air outlet is arranged at the top of the cyclone cutter air inlet part, the air outlet is connected with an air suction pump 5 through a hose, and the controlled end of the air suction pump 5 is connected with the output end of the logic controller; the cyclone cutter gas quality separation part is a conical barrel type with a dust falling port arranged at the bottom.

[0014] Preferably, the rotating platform assembly includes a rotating platform stepper motor fixed on the lower shell plate of the shell, the rotating shaft of the rotating platform stepper motor is vertically upward and horizontally supported by a support with a glass plate located below the dust falling port, and the controlled end of the rotating platform stepper motor is connected with the output end of the logic controller.

[0015] Preferably, a screw base is arranged on the bottom shell plate of the shell, a screw is vertically upward arranged on the screw base, and a main support located above the glass plate is penetrated on the screw;

[0016] The wrapping assembly comprises a medium processing support for accommodating wrapping medium and a medium rubber blade for scraping the wrapping medium into a thin film attached on the glass plate; the bottom of the medium processing support is provided with an accumulation frame, and the bottom of the accumulation frame is attached to the upper surface of the glass plate; the top of the medium processing support is fixed on the main support, and the top of the medium processing support is provided with a first entering hole communicating with the accumulation frame, and the first entering hole is connected with the wrapping medium through a peristaltic pump, and the controlled end of the peristaltic pump is connected with a logic controller; the medium rubber blade is fixed on one side end of the medium processing support and attached to the upper surface of the glass plate.

[0017] The accumulation assembly comprises a blade and a magnet; the blade is arranged on the upper surface of the glass plate through a blade support fixed on the lower surface of the main support and is obliquely cut with the glass plate; and the magnet is arranged below the glass plate through a magnet support fixed on the screw rod and is located in the same vertical plane as the blade.

[0018] Preferably, the wrapping medium is glycerol.

[0019] Preferably, the main support is provided with a cleaning assembly for cleaning the observed aerosol particle sample, and the cleaning assembly comprises a cleaning rubber blade and a leakage receiving box; the cleaning rubber blade is fixed on the main support through a cleaning support and is attached to the upper surface of the glass plate; and the leakage receiving box is arranged below the edge of the glass plate corresponding to the cleaning rubber blade.

[0020] Preferably, the dust falling port of the cyclone cutter, the objective lens of the microscope, the wrapping assembly, the accumulation assembly and the cleaning assembly are located on the same circular cylindrical surface perpendicular to the glass plate.

[0021] The low-cost air-borne pollen online observation method with high precision, specifically comprising the following steps:

[0022] S1: Preparation: glycerol is dropped to the A area of the glass plate through the medium processing support, the glass plate is rotated clockwise by the rotary platform stepping motor, and the glycerol is scraped into a thin layer on the A area of the glass plate by the medium rubber blade; and the glass plate continues to rotate until the A area rotates below the dust falling port of the cyclone cutter;

[0023] S2: Sampling: air is sucked from the six air inlets around the top of the cyclone cutter, pollen-containing particles in the air fall to the A area of the glass plate covered with a glycerol thin layer from the bottom dust falling port as a sample, and move to the blade along with the continuous rotation of the glass plate driven by the rotary platform stepping motor.

[0024] S3: Accumulation: the blade is obliquely cut on the glass plate with an obtuse angle with one side of the sample, and the sample in the A area of the glass plate is accumulated at the blade edge along the tangent line of the blade and the glass plate, and moves below the objective lens of the microscope along with the continuous rotation of the glass plate driven by the rotary platform stepping motor.

[0025] S4: Observation work: the objective lens is directly above the blade edge of the blade, the image sensor observes the sample gathered at the blade edge of the blade through the objective lens, while adjusting the device stepping motor to drive the X / Y / Z axis precision displacement platform to drive the image sensor and the objective lens to reciprocate up and down on the order of μm to focus on the sample, and to identify through the XX method;

[0026] S5: Cleaning work: after the sample is observed, the rotating platform stepping motor drives the continuous rotation of the glass plate, moves to the cleaning rubber blade, is gathered by the cleaning rubber blade, and is scraped out from the side of the glass plate, and the cleaning rubber blade simultaneously cleans the A area of the glass plate;

[0027] S6: Repeat observation: after the cleaning of the glass plate is completed, the rotating platform stepping motor drives the glass plate to move to the medium treatment support again, completes an observation period and starts the next observation period.

[0028] Preferably, the A area: in single observation, the A area can be regarded as a point on the glass plate; in continuous observation, the A area can be regarded as a circular area on the glass plate, the part of the glass plate directly opposite the dust falling port of the cyclone cutter, the medium rubber blade, the part of the blade initially in contact with the sample, the medium treatment support and the cleaning rubber blade are in the area;

[0029] The observation period: in single observation, the one-week movement of the A area on the glass plate can be regarded as an observation period; and in continuous observation, every moment is the beginning and end of an observation period.

[0030] Thanks to the above technical solutions, the technical progress achieved by the application is as follows.

[0031] The main equipment components and parts of the application are selected from common and mature products on the market, which are low in cost, so that the equipment is easy to obtain through manufacturing; the overall structure of the application is relatively simple, and after precise installation and debugging, the post-maintenance amount is small and no excessive daily operation is required, various instrument operations and sample analysis control can be remotely operated through a computer and software, which not only facilitates widespread deployment and use, but also greatly reduces the loss of manpower and resources; the measurement principle and method of the application determine that the equipment itself has high observation precision, so that on the premise of high observation precision, the accumulation of continuous observation results can be focused on, the software model is optimized through AI training in the software aspect, and the observation quality, pollen identification ability and the like are further improved. BRIEF DESCRIPTION OF DRAWINGS

[0032] Figure 1 is a perspective view of the application;

[0033] Figure 2 is a top view of the application;

[0034] Figure 3 is a side view of the present invention;

[0035] Figure 4 is an internal perspective view of the present invention;

[0036] Figure 5 is an internal top view of the present invention;

[0037] Figure 6 is an internal side view of the present invention;

[0038] Figure 7 is a perspective view of the rotating platform assembly of the present invention;

[0039] Figure 8 is a side view of the rotating platform assembly of the present invention;

[0040] Figure 9 is a schematic view of the cyclone cutter of the present invention;

[0041] Figure 10 is a schematic view of the main support of the present invention;

[0042] Figure 11 is an assembly view of the gathering assembly and main support of the present invention;

[0043] Figure 12 is a schematic view of the wrapping assembly of the present invention;

[0044] Figure 13 is a schematic view of the cleaning assembly of the present invention;

[0045] Figure 14 is a schematic view of the adjustment device of the present invention;

[0046] Figure 15 is an assembly view of the microscope and image sensor of the present invention;

[0047] Figure 16 is a perspective view of the LTE light source of the present invention;

[0048] Figure 17 is a schematic view of the electrical control of the present invention;

[0049] Figure 18 is a block diagram of the principles of the present invention.

[0050] Wherein: 1. housing, 2. AC power input plug, 3. DC power output plug, 4. signal output plug, 5. air pump, 6. cyclone cutter, 61. air outlet, 62. airflow exciting air inlet, 63. main air inlet, 64. cyclone cutter air inlet part, 65. cyclone cutter air quality separation part, 66. dust fall port, 7. peristaltic pump, 8. main control board, 9. rotating platform stepper motor controller, 10. adjustment device stepper motor controller, 11. switching power supply, 12. image sensor top cover, 121. image sensor data line port, 13. image sensor support, 14. lens barrel, 15. objective lens clamp, 16. objective lens, 17. LED light source, 18. image sensor, 19. screw rod, 20. screw rod base, 21. blade support, 211. blade support positioning hole, 22. magnet support, 221. magnet positioning hole, 23. blade, 24. medium processing support, 241. first entering hole, 242. medium processing support positioning hole, 25. medium rubber wiper, 26. cleaning support, 261. cleaning support positioning hole, 27. cleaning rubber wiper, 28. main support, 281. second entering hole, 282. positioning hole, 283. medium rubber wiper adjustment hole, 29. glass plate, 291. positioning hole, 30. glass plate support, 31. rotating support, 32. rotating platform stepper motor support, 33. rotating platform stepper motor, 34. X / Y / Z axis precision displacement platform, 35. lens barrel clamp, 36. sliding positioning block, 37. adjustment device stepper motor support, 38. adjustment device stepper motor, 39. first connecting column, 40. second connecting column. DETAILED DESCRIPTION

[0051] The application will be further described in detail below in combination with the drawings and specific embodiments.

[0052] A low-cost air pollen online observation device with high precision, combined with Figures 1 to 6As shown, it comprises a shell 1, an aerosol particle collecting device is arranged on the shell 1, and the inside of the shell 1 is provided with a rotating platform assembly, a wrapping assembly, an aggregation assembly, a cleaning assembly and an imaging acquisition assembly, wherein the aerosol particle collecting device is used for collecting aerosol particles containing pollen in the air; the rotating platform assembly is arranged below the aerosol particle collecting device and is used for carrying and conveying the aerosol particles collected by the aerosol particle collecting device; the wrapping assembly is used for wrapping the aerosol particles and is arranged on the rotating platform assembly; the aggregation assembly is used for aggregating the wrapped aerosol particles and is arranged on the rotating platform assembly; the imaging acquisition assembly is used for observing the aerosol particle sample after being wrapped and aggregated, is arranged above one side of the rotating platform and can adjust the observation angle; and the cleaning assembly is used for cleaning the sample after being observed and is arranged on the rotating platform assembly. The aerosol particle collecting device, the rotating platform assembly, the wrapping assembly and the imaging acquisition assembly are connected with a logic controller, and the logic controller is used for automatically controlling the online observation of the airborne pollen.

[0053] The aerosol particle collecting device is a cyclone cutter 6 (other devices capable of collecting aerosol particles can also be used), which is longitudinally arranged through the upper shell plate of the shell 1. As shown in Figure 9 The cyclone cutter 6 comprises a cyclone cutter air inlet part 64 and a cyclone cutter air quality separation part 65, wherein the cyclone cutter air inlet part 64 is fixedly connected with the upper shell plate of the shell 1, and the top of the cyclone cutter air inlet part 64 is exposed outside the shell 1; three main air inlets 63 and three airflow excitation air inlets 62 are arranged around the top of the cyclone cutter air inlet part 64, the three main air inlets 63 are distributed at an angle of 120°, the three airflow excitation air inlets 62 are distributed at an angle of 120°, and the main air inlets 63 and the airflow excitation air inlets 62 are arranged in an alternating circumferential manner; an air outlet 61 is arranged at the top of the cyclone cutter air inlet part 64, and a suction pump 5 is connected to the air outlet 61 through a hose, the suction pump 5 is used to provide power for the cyclone cutter 6 to collect aerosol particles, and the exhaust port of the suction pump 5 is arranged outside or below the shell 1, so as to avoid interfering with the air inlet of the cyclone cutter 6. The cyclone cutter air quality separation part 65 is combined with the cyclone cutter air inlet part 64, the cyclone cutter air quality separation part 65 is located inside the shell 1, the cyclone cutter air quality separation part 65 is in the shape of a tapered barrel, and a dust falling port 66 is arranged at the bottom. In use, the suction pump 5 sucks air at a constant flow rate, and particles (including pollen particles) in the atmosphere enter the cyclone cutter 6 from the air inlets under the action of suction and fall from the dust falling port 66.

[0054] As shown in Figures 7 to 8As shown, the rotating platform assembly comprises a rotating platform stepping motor 33 and a glass plate 29 (which can be replaced by other materials with similar properties), wherein the rotating platform stepping motor 33 is fixed on the lower shell plate of the shell 1 through a rotating platform stepping motor support 32, the rotating shaft of the rotating platform stepping motor 33 is upwardly arranged, a horizontal rotating support 31 is fixed on the rotating shaft of the rotating platform stepping motor 33, a glass plate support 30 is placed on the rotating support 31, the glass plate 29 is placed on the glass plate support 30, a positioning hole 291 is formed on the glass plate 29, and the glass plate 29 is fixedly connected with the glass plate support 30. The glass plate 29 is arranged below the dust falling port 66 and is used for carrying the aerosol particles discharged from the dust falling port 66, and the glass plate 29 is driven to rotate by the rotating platform stepping motor 33.

[0055] The imaging acquisition assembly comprises an imaging acquisition device and an adjusting device, wherein the adjusting device is used for adjusting the observation angle of the imaging acquisition device, such as Figure 14 As shown, the adjusting device comprises an adjusting device stepping motor 38 and an X / Y / Z axis precision displacement platform 34, the adjusting device stepping motor 38 is arranged on the side of the rotating platform assembly through an adjusting device stepping motor support 37 arranged in the shell 1, the rotating shaft of the adjusting device stepping motor 38 is upwardly arranged, a transmission component is arranged on the rotating shaft of the adjusting device stepping motor 38, the transmission component comprises two sliding positioning blocks 36, the two sliding positioning blocks 36 are connected through a second connecting column 40 arranged therebetween; the X / Y / Z axis precision displacement platform 34 is fixed on the sliding positioning block 36 and is located above the side of the rotating platform assembly, and a lens barrel clamp 35 located above the rotating platform is connected to the X / Y / Z axis precision displacement platform 34 through a first connecting column 39.

[0056] As shown in the figure Figures 15 to 16As shown, the imaging acquisition assembly comprises a microscope, an LED light source 17 and an image sensor 19, wherein the microscope comprises an objective lens 16 and a lens barrel 14, the objective lens 16 and the lens barrel 14 are connected through an objective lens clamp 15, the lens barrel 14 is clamped longitudinally on the lens barrel clamp 35, and the objective lens 16 is arranged towards the rotating platform assembly; the LED light source 17 is mounted on an LED heat sink and arranged on the bottom shell plate of the shell 1 through an LED support and arranged towards the objective lens 16, an LED lens is buckled on the LED light source 17, and the LED lens can play a role of light condensation; the image sensor 18 can be a CCD sensor or a CMOS sensor, and is arranged on the top of the lens barrel 14 through an image sensor support 13 arranged on the upper part of the lens barrel 14, an image sensor top cover 12 is buckled on the image sensor 18, the image sensor top cover 12 can play a role of covering and protecting the image sensor 18, the image sensor top cover 12 is provided with an image sensor data line port 121, the shell 1 is provided with a signal output plug 4, the image sensor data line port 121 is connected with the signal output plug 4 through a signal line, so as to realize transmission of collected image information to an external device.

[0057] The LED lens, the objective lens 16 and the image sensor 18 are on the same vertical line, in use, the adjusting device stepper motor 38 is connected with the X / Y / Z axis precision displacement platform 34 through the transmission device and the guide rail on the X / Y / Z axis precision displacement platform 34, so as to convert the rotary motion of the adjusting device stepper motor 38 into the axial movement of the X / Y / Z axis precision displacement platform 34, and then drive the imaging acquisition assembly to move in the X / Y / Z axis direction, and finally complete the image acquisition of the sample on the rotating platform assembly.

[0058] As shown in Figure 10 The bottom shell plate of the shell 1 is provided with four screw rod bases 20, the screw rod bases 20 are arranged in a rectangular shape, the screw rod bases 20 are vertically upwardly provided with screw rods 19, and the screw rods 19 are provided with main supports 28. The main supports 28 are threadedly connected with the screw rods 19 and located above the glass plate 29, and the wrapping assembly, the gathering assembly and the cleaning assembly are arranged on the main supports 28.

[0059] As shown in Figure 12As shown, the wrapping assembly comprises a medium processing support 24 and a medium rubber blade 25, wherein the bottom of the medium processing support 24 is provided with an accumulation frame, the bottom of the accumulation frame is attached to the upper surface of the glass plate 29; the top of the medium processing support 24 is provided with a medium processing support positioning hole 242, the main support 28 is provided with a positioning hole 282, the positioning hole 282 and the medium processing support positioning hole 242 are matched, so as to fix the medium processing support 24; the top of the medium processing support 24 is also provided with a first entering hole 241, the main support 28 is provided with a second entering hole 281 at a position corresponding to the first entering hole 241, the first entering hole 241 and the second entering hole 281 are communicated with the accumulation frame, and the first entering hole 241 is connected with wrapping medium (the wrapping medium can be replaced by glycerol or other similar medium, and the medium can illuminate aerosol particles through the refraction of light in the medium) through the peristaltic pump 7. The medium rubber blade 25 is arranged at one side end of the medium processing support 24 and is fixedly connected with the main support 28 through the medium rubber blade adjusting hole 283 provided on the main support 28, the medium rubber blade 25 is attached to the upper surface of the glass plate 29, and the medium rubber blade 25 is used to scrape the wrapping medium into a film and attach the film to the glass plate 29.

[0060] In use, the liquid suction pipe of the peristaltic pump 7 extracts glycerol at a speed of the order of ml / min, the liquid outlet pipe of the peristaltic pump 7 passes through the first entering hole 241 and the second entering hole 281 and drops into the accumulation frame of the medium processing support 24, and with the accumulation of a certain time, the glycerol is spread on the glass plate 29 of the rotating platform within the range framed by the accumulation frame of the medium processing support 24. Finally, the glass plate 29 of the rotating platform drives the glycerol to be uniformly output from the outlet of the medium processing support 24 to the outside of the accumulation frame of the medium processing support 24 in the rotating process, and the glycerol film is formed by the secondary scraping of the medium rubber blade 25 and is attached to the glass plate 29 of the rotating platform.

[0061] As Figure 11As shown, the gathering assembly includes a blade holder 21, a blade 23, a magnet holder 22 and a magnet, wherein the blade holder 21 is fixed on the lower surface of the main holder 28, the blade 23 is fixed on the blade holder 21 through the blade holder positioning hole 211 and is obliquely cut with the glass plate 29; the magnet holder 22 is fixed on the screw rod 19 and is located below the glass plate 29 and away from the glass plate 29 by a distance, the magnet holder 22 is provided with a magnet positioning hole 221, and the magnet is fixed on the magnet positioning hole 221; the blade 23 and the magnet are located in the same vertical plane, and the magnet can strengthen the scraping force of the blade 23 on the glass plate 29. In use, the glass plate 29 of the rotating platform carrying the glycerol sample wrapped with aerosol particles (containing pollen) is rotated to the blade 23, and the blade 23 gathers the glycerol sample at the position where the blade 23 is tangent to the glass plate 29 of the rotating platform; finally, with the continuous rotation of the glass plate 29 of the rotating platform, the gathered glycerol sample is gradually moved into the area below the objective lens 16 of the imaging acquisition assembly, which is less than 1 mm. 2

[0062] The cleaning assembly includes a cleaning rubber blade 27 and a leakage receiving box, as shown in the figure, the cleaning rubber blade 27 is fixed on the cleaning holder 26, the cleaning holder 26 is fixed on the main holder 28 through the cleaning holder positioning hole 261 and ensures that the cleaning rubber blade 27 is in contact with the upper surface of the glass plate 29, and the cleaning rubber blade 27 is wrapped with a cleaning cloth; the leakage receiving box is arranged below the edge of the glass plate 29 corresponding to the cleaning rubber blade 27, and a garbage bag is arranged in the leakage receiving box. In use, with the rotation of the glass plate 29 of the rotating platform, the glycerol sample observed after the glycerol sample wrapped with aerosol particles (containing pollen) is rotated to the cleaning rubber blade 27 and the cleaning cloth, collected and wiped to the edge of the glass plate 29 of the rotating platform, and finally falls into the garbage bag of the leakage receiving box. Figure 13

[0063] The output end of the logic controller is connected with the rotating platform stepping motor controller 9, the adjusting device stepping motor controller 10, the flow controller and the controlled end of the LED light source 17 arranged in the shell 1, wherein the output end of the rotating platform stepping motor controller 9 is connected with the controlled end of the rotating platform stepping motor 33; the output end of the adjusting device stepping motor controller 10 is connected with the controlled end of the adjusting device stepping motor 38; the output end of the flow controller is connected with the controlled end of the air suction pump 5 and the peristaltic pump 7; and the input end of the logic controller is connected with the output end of the image sensor 18, so as to realize the automatic control of the online observation of the air-borne pollen.

[0064] ​​The device further comprises an electrical assembly for providing power to the device. The electrical assembly comprises a switching power supply 11, a main control board 8, an AC power input plug 2 and a DC power output plug 3, wherein the switching power supply 11 is arranged inside the housing 1, the switching power supply 11 is internally provided with a power module; the AC power input plug 2 and the DC power output plug 3 are arranged on the housing; and the main control board 8 is arranged inside the housing 1.

[0065] The electrical control schematic diagram is shown in Figure 17 The AC power input plug 2 is connected to the power input end of the power module in the switching power supply 11, and the power module converts the introduced 220V AC power into DC power with different voltage values; the output end of the power module is connected to the DC power output plug 3, the logic controller and the main control board 8, the DC power output plug 3 leads out the DC power from the device and is connected to the air suction pump 5 and the peristaltic pump 7 respectively. The main control board 8 is connected to the flow controller, the image sensor 18, the LED light source 17, the rotary platform stepping motor controller 9 and the adjustment device stepping motor controller 10, thereby playing a role in power supply and signal path.

[0066] A low-cost air-borne pollen online observation method with high precision is realized based on a low-cost air-borne pollen online observation device with high precision, and the principle is shown in Figure 18 The method comprises the following steps:

[0067] S1: Preparation: glycerol is dropped onto the A area of the glass plate 29 through the medium processing support 24, the rotary platform stepping motor 33 drives the glass plate 29 to rotate clockwise, the medium rubber blade 25 scrapes the glycerol into a thin layer on the A area of the glass plate 29, and the glass plate 29 continues to rotate until the A area rotates to below the dust falling port 66 of the cyclone cutter 6;

[0068] S2: Sampling work: air is sucked from the six air inlets around the top of the cyclone cutter 6, pollen-containing particulate matters in the air fall onto the A area of the glass plate 29 covered with a thin layer of glycerol from the bottom dust falling port 66 as a sample, and move to the blade 23 along with the continuous rotation of the glass plate 29 driven by the rotary platform stepping motor 33.

[0069] S3: Gathering work: the blade 23 obliquely cuts on the glass plate 29, and the angle between the blade 23 and the sample on one side is an obtuse angle, the sample on the A area of the glass plate 29 is gathered at the cutting edge of the blade 23 (at this time the sample is separated from the A area), and moves to below the objective lens 16 of the microscope along the tangent line of the blade 23 and the glass plate 29 along with the continuous rotation of the glass plate 29 driven by the rotary platform stepping motor 33;

[0070] S4: Observation work: The objective lens 16 is directly above the blade edge of the blade 23, the image sensor 18 observes the sample gathered at the blade edge of the blade 23 through the objective lens 16, while adjusting the device stepping motor 38 to drive the X / Y / Z axis precision displacement platform 34 to drive the image sensor 18 and the objective lens 16 to reciprocate in the order of μm to focus on the sample, and to identify through YOLO series, SSD and Faste RCNN and other artificial intelligence-based target detection algorithms;

[0071] S5: Cleaning work: After the sample is observed, the rotating platform stepping motor 33 drives the continuous rotation of the glass plate 29, moves to the cleaning rubber blade 27, is gathered by the cleaning rubber blade 27, and is scraped out from the side of the glass plate 29, and the cleaning rubber blade 27 cleans the A area of the glass plate 29 at the same time;

[0072] S6: Repeat observation: After the cleaning of the glass plate 29, the rotating platform stepping motor 33 drives it to move to the medium processing support 24 again, completes an observation period and starts the next observation period.

[0073] Among them, the A area: in single observation, the A area can be regarded as a certain point on the glass plate 29; in continuous observation, the A area can be regarded as a circular area on the glass plate 29, the part of the glass plate 29 directly opposite the dust falling port 66 of the cyclone cutter 6, the medium rubber blade 25, the blade 23 and the initial contact part of the sample, the medium processing support 24 and the cleaning rubber blade 27 are in the area.

[0074] Observation period: in single observation, the one-week movement of the A area on the glass plate 29 can be regarded as an observation period; while in continuous observation, every moment is the beginning and end of an observation period.

Claims

1. Low cost air pollen online observation device with high precision, comprising a housing (1), characterized in that: The shell (1) is provided with an aerosol particle collecting device for collecting aerosol particles containing pollen in the air; the inside of the shell (1) is provided with a rotating platform assembly below the aerosol particle collecting device for carrying and transporting the aerosol particles collected by the aerosol particle collecting device; the rotating platform assembly is provided with a wrapping assembly for wrapping the aerosol particles and an aggregation assembly for aggregating the wrapped aerosol particles, and an imaging acquisition assembly for observing the wrapped and aggregated aerosol particle sample is provided above one side of the rotating platform; the aerosol particle collecting device, the rotating platform assembly, the wrapping assembly and the imaging acquisition assembly are connected with a logic controller for automatically controlling the online observation of airborne pollen; The aerosol particle collecting device is a cyclone cutter (6) longitudinally penetrating the upper shell plate of the shell (1), the cyclone cutter (6) includes a cyclone cutter air inlet part (64) fixedly connected with the upper shell plate of the shell (1) and exposed outside the shell (1) and a cyclone cutter gas quality separation part (65) located inside the shell (1) and combinedly connected with the cyclone cutter air inlet part (64); three main air inlets (63) and three airflow exciting air inlets (62) are arranged around the top of the cyclone cutter air inlet part (64) at an interval of 120°, the main air inlets (63) and the airflow exciting air inlets (62) are arranged in an alternating circumferential arrangement, and the main air inlets (63) and the airflow exciting air inlets (62) are arranged at different heights; an air outlet (61) is arranged at the top of the cyclone cutter air inlet part (64), the air outlet (61) is connected with a suction pump (5) through a hose, and a controlled end of the suction pump (5) is connected with an output end of the logic controller; the cyclone cutter gas quality separation part (65) is a conical barrel type with a dust falling port (66) arranged at the bottom; The rotating platform assembly includes a rotating platform stepping motor (33) fixed on the lower shell plate of the shell (1), a rotating shaft of the rotating platform stepping motor (33) is vertically upwardly arranged and horizontally supported by a support with a glass plate (29) located below the dust falling port (66), and a controlled end of the rotating platform stepping motor (33) is connected with an output end of the logic controller; A screw base (20) is arranged on the bottom shell plate of the shell (1), a screw (19) is vertically upwardly arranged on the screw base (20), and a main support (28) is penetrated through the screw (19) and located above the glass plate (29); The wrapping assembly comprises a medium processing support (24) for accommodating wrapping medium and a medium rubber blade (25) for scraping the wrapping medium to be attached to the glass plate (29) as a film; the bottom of the medium processing support (24) is provided with an accumulation frame, and the bottom of the accumulation frame is attached to the upper surface of the glass plate (29); the top of the medium processing support (24) is fixed on the main support (28), and the top of the medium processing support (24) is provided with a first entering hole (241) communicating with the accumulation frame, and the first entering hole (241) is connected with the wrapping medium through a peristaltic pump (7), and the controlled end of the peristaltic pump (7) is connected with a logic controller; the medium rubber blade (25) is fixed on one side end of the medium processing support (24) and attached to the upper surface of the glass plate (29); The accumulation assembly comprises a blade (23) and a magnet; the blade (23) is arranged on the upper surface of the glass plate (29) through a blade support (21) fixed on the lower surface of the main support (28) and is obliquely cut with the glass plate (29); the magnet is arranged below the glass plate (29) through a magnet support (22) fixed on the screw rod (19) and is located in the same vertical plane as the blade (23); The main support (28) is provided with a cleaning assembly for cleaning the observed aerosol particle sample, and the cleaning assembly comprises a cleaning rubber blade (27) and a leakage receiving box; the cleaning rubber blade (27) is fixed on the main support (28) through a cleaning support (26) and is attached to the upper surface of the glass plate (29); the leakage receiving box is arranged below the edge of the glass plate (29) corresponding to the cleaning rubber blade (27); The dust falling port (66) of the cyclone cutter (6), the objective lens (16) of the microscope, the wrapping assembly, the accumulation assembly and the cleaning assembly are located on the same circular cylindrical surface perpendicular to the glass plate (29).

2. The low-cost, high-precision, airborne pollen online observation device according to claim 1, characterized in that: The imaging acquisition assembly comprises an imaging acquisition device and an adjusting device for adjusting the observation angle of the imaging acquisition device; The adjusting device comprises an adjusting device stepping motor (38) and an X / Y / Z axis precision displacement platform (34); the adjusting device stepping motor (38) is arranged on the side of the rotating platform assembly with the rotating shaft upward, and the controlled end of the adjusting device stepping motor (38) is connected with the output end of the logic controller; the X / Y / Z axis precision displacement platform (34) is arranged above the side of the rotating platform assembly through a transmission part connected with the rotating shaft of the adjusting device stepping motor (38), and the X / Y / Z axis precision displacement platform (34) is provided with a lens barrel clamp (35) located above the rotating platform; The imaging acquisition device comprises a microscope, an LED light source (17) and an image sensor (18), the microscope comprises an objective lens (16) and a lens barrel (14) connected with the objective lens (16) through an objective lens clamp (15), the lens barrel (14) is clamped longitudinally on a lens barrel clamp (35), and the objective lens (16) is arranged towards the rotating platform assembly; the LED light source (17) is arranged on a bottom shell plate of the shell (1) and towards the objective lens (16), a controlled end of the LED light source (17) is connected with an output end of the logic controller, and an LED lens for light collection is buckled on the LED light source (17); the image sensor (18) is arranged on the top of the lens barrel (14) through an image sensor support (13) arranged on the upper portion of the lens barrel (14), and an output end of the image sensor (18) is connected with an input end of the logic controller; the LED lens, the objective lens (16) and the image sensor (18) are on the same vertical line.

3. The low-cost, high-precision, airborne pollen online observation device according to claim 1, characterized in that: The wrapping medium is glycerol.

4. A low-cost method for online observation of airborne pollen with high precision, characterized in that: The observation method is realized by using the low-cost air pollen online observation equipment with high precision in any one of claims 1 to 3, and specifically comprises the following steps: S1: preparation: glycerol is dropped on the A area of the glass plate (29) through the medium processing support (24), the rotating platform stepping motor (33) drives the glass plate (29) to rotate clockwise, the medium rubber blade (25) scrapes the glycerol into a thin layer on the A area of the glass plate (29), and the glass plate (29) continues to rotate until the A area rotates to below the dust falling port (66) of the cyclone cutter (6); S2: sampling work: air is sucked from six air inlet ports around the top of the cyclone cutter (6), pollen-containing particles in the air fall on the A area of the glass plate (29) covered with a glycerol thin layer through the bottom dust falling port (66) as a sample, and move to the blade (23) along with the continuous rotation of the glass plate (29) driven by the rotating platform stepping motor (33); S3: gathering work: the blade (23) is obliquely cut on the glass plate (29) and forms an obtuse angle with one side of the sample, the sample on the A area of the glass plate (29) is gathered at the blade edge (at this time the sample is separated from the A area) by the blade (23) and moves to below the objective lens (16) of the microscope along the tangent line of the blade (23) and the glass plate (29) along with the continuous rotation of the glass plate (29) driven by the rotating platform stepping motor (33); S4: observation work: the objective lens (16) is directly above the blade edge of the blade (23), the image sensor (18) observes the sample gathered at the blade edge of the blade (23) through the objective lens (16), adjusts the X / Y / Z axis precision displacement platform (34) driven by the device stepping motor (38) to drive the image sensor (18) and the objective lens (16) to make up-down reciprocating motion in the order of μm to focus on the sample, and identifies through the YOLO series, SSD and Faste RCNN artificial intelligence target detection algorithm. S5: Cleaning: the observed sample, with the continuous rotation of the glass plate (29) driven by the rotary platform stepper motor (33), moves to the cleaning rubber blade (27), is gathered by the cleaning rubber blade (27), and is scraped out from the side of the glass plate (29), and the cleaning rubber blade (27) cleans the A area of the glass plate (29) at the same time; S6: Repeat observation: the cleaned glass plate (29) is moved to the medium treatment support (24) again driven by the rotary platform stepper motor (33), a observation cycle is completed and the next observation cycle begins.

5. The low-cost air pollen online observation method with high precision according to claim 4, characterized in that: The A area: in single observation, the A area can be regarded as a point on the glass plate (29); in continuous observation, the A area can be regarded as a circular area on the glass plate (29), and the parts of the glass plate (29) opposite to the dust falling port (66) of the cyclone cutter (6), the medium rubber blade (25), the blade (23) and the sample initial contact part, the medium treatment support (24) and the cleaning rubber blade (27) are in the area; The observation cycle: in single observation, the one-week movement of the A area on the glass plate (29) can be regarded as an observation cycle; and in continuous observation, every moment is the beginning and end of an observation cycle.

Images