Particle detection device

Abstract

The present invention relates to a particle detection device, comprising a light source module, a carrier plate, a mixing module, and a camera module. A sample consumable plate is placed on the carrier plate, and a sample to be detected is injected into the sample consumable plate. The mixing module blows and mixes the sample injected into the sample consumable plate. The light source module provides light, which is focused on the sample consumable plate. The light passes through an objective lens and is received by a camera. The sample is blown and mixed by the mixing module to prevent the particles of the sample from settling, thereby improving the detection accuracy. The camera then takes a photo of the sample consumable plate, realizing automated operation throughout the process. The particle detection device is not only simple to operate, but also can prevent the particles from settling, thereby improving the detection accuracy.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a particle detection device. Background Art

[0002] Many modern techniques in biochemistry and biotechnology are based on the analysis of biological particles, such as cells. Various parameters related to the type and species of the particles, as well as their state, such as viability, are among the parameters and properties routinely studied. Further information regarding the intracellular state is also frequently sought. In this field, luminescence detection methods, such as fluorescence detection, have gained widespread application, primarily due to their inherent specificity and sensitivity.

[0003] Currently, a commonly used automated analyzer is used to analyze particles under flow conditions, typically analyzing a single particle at a time. Some types of analyzers can perform complex analyses of captured biological particles, but these instruments often require considerable operator skill and are difficult to use.

[0004] Another type of particle detection device available on the market uses a 96-well plate to count particles by tapping the particles within the container column. However, particle counts measured using a 96-well plate are less accurate and the analyzed particles are less realistic because laboratories batch particle samples into the 96-well plate and then perform batch testing, causing the particles to settle to the bottom of the container. Summary of the Invention

[0005] Based on this, it is necessary to provide a particle detection device that is easy to operate and can prevent particle sedimentation to improve detection accuracy in order to address the above technical problems.

[0006] A particle detection device, comprising:

[0007] a light source module, comprising at least one light source for illuminating the sample;

[0008] A carrying plate, the carrying plate is used to carry the sample consumable plate, and the carrying plate is movable;

[0009] A mixing module, used to mix the sample injected into the sample consumable plate; and

[0010] The camera module comprises an objective lens, and the carrying plate is located between the light source module and the objective lens.

[0011] In one embodiment, the light source module includes at least two light sources, a first power source, a first switching device and a light source shaping system, the at least two light sources are arranged at intervals on the first switching device, the first power source is used to drive the first switching device to rotate relative to the objective lens, and the light source shaping system is located above the light source.

[0012] In one embodiment, the particle detection device further includes an X-axis module and a Y-axis module, and the X-axis module and the Y-axis module move along the carrier plate in the X-axis or Y-axis direction.

[0013] In one embodiment, the supporting plate may be further disposed on the Y-axis module, and the supporting plate moves relative to the objective lens via the Y-axis module.

[0014] In one embodiment, the X-axis module may be disposed on a supporting frame, and the Y-axis module and the supporting plate may move relative to the objective lens via the X-axis module.

[0015] In one embodiment, the camera module further includes a camera, which is located above the objective lens and is used to take pictures of the sample consumable plate carried on the carrying plate.

[0016] In one embodiment, the photographic module further includes a second power source, a second switching device and at least two filters, wherein the at least two filters are spaced apart on the second switching device, and the second power source is used to drive the second switching device to rotate relative to the objective lens, and the second switching device is located between the objective lens and the camera.

[0017] In one embodiment, the camera module further includes a tube lens, and the tube lens is located between the second switching device and the camera.

[0018] In one embodiment, the particle detection device further includes a mixing module, and the mixing module is used to mix or dye the sample injected into the sample consumable plate.

[0019] In one embodiment, the sample consumable plate includes a substrate, a sampling device and a suction device, the sampling device and the suction device are arranged on the substrate, a flow channel is embedded in the substrate, the suction device is connected to the sampling device through the flow channel, the mixing module is connected to the suction device and sucks the suction device, so that the sample in the sampling device enters the flow channel for staining.

[0020] In one embodiment, the mixing module includes a third power source, an exhaust pump and a first needle, the first needle is connected to the exhaust pump, and the third power source is used to drive the exhaust pump to operate so as to exhaust the suction device of the sample consumable plate through the first needle, thereby allowing the sample in the sampling device of the sample consumable plate to pass through the flow channel for staining and flow into the detection window.

[0021] In one embodiment, the third power source is a first linear stepper motor, the aspirator includes a first cylinder body and a first piston rod, one end of the first piston rod is arranged in the first cylinder body, the other end of the first piston rod is arranged at the output end of the first linear stepper motor, and the first needle is connected to the first cylinder body.

[0022] In one embodiment, the particle detection device further includes a first mounting member and a first driving member, wherein the first mounting member is arranged on the first driving member, and the first needle is arranged on the first mounting member, and the first driving member is used to drive the first mounting member to move so that the first needle is inserted into the vacuum device of the sample consumable plate.

[0023] In one embodiment, the sample consumable plate includes a substrate, a sampling device and a blowing and suction device, the sampling device and the blowing and suction device are arranged on the substrate, the substrate is embedded with a flow channel, the blowing and suction device is connected to the sampling device through the flow channel, the mixing module is connected to the blowing and suction device and blows or draws air to the blowing and suction device, so that the sample in the sampling device flows into the blowing and suction device through the flow channel or the sample in the blowing and suction device flows into the sampling device through the flow channel, so that the sample is mixed.

[0024] In one embodiment, the mixing module includes a fourth power source, a blower and a second needle, the second needle is connected to the blower, and the second needle is used to be inserted into the blowing and suction device on the sample consumable plate. The fourth power source is used to drive the blower to blow or draw air from the blowing and suction device of the sample consumable plate through the second needle, thereby mixing the sample in the sampling device of the sample consumable plate.

[0025] In one embodiment, the blowing and suction power source is a second linear stepping motor, and the blower includes a second cylinder and a second piston rod, one end of the second piston rod is arranged in the second cylinder, and the other end of the second piston rod is arranged at the output end of the second linear stepping motor; the second needle is connected to the second cylinder.

[0026] In one embodiment, the particle detection device further includes a second mounting member and a second driving member, the second mounting member is arranged on the second driving member, the second needle is arranged on the second mounting member, and the second driving member is used to drive the second mounting member to move so that the second needle is inserted into the blowing and suction device of the sample consumable plate.

[0027] The above particle detection device has at least the following advantages:

[0028] The sample plate is placed on a carrier plate and the sample to be tested is injected into the plate. The mixing module pipettes the sample into the plate to mix it evenly. The light source module provides light, which is focused on the plate and then passes through the objective lens and is received by the camera. Pipetting and mixing the sample by the mixing module prevents particle sedimentation, improving detection accuracy. The camera then takes a picture of the sample plate, fully automating the entire process. This particle detection device is not only simple to operate but also prevents particle sedimentation, improving detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The drawings constituting a part of this application are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0031] Figure 1 is a schematic diagram of a partial assembly of a particle detection device in one embodiment;

[0032] Figure 2 for Figure 1 An exploded view of the particle detection device shown;

[0033] Figure 3 for Figure 1 A front view of the particle detection device shown;

[0034] Figure 4 for Figure 1 A side view of the particle detection device shown;

[0035] Figure 5 for Figure 1 A top view of the particle detection device shown;

[0036] Figure 6 is a top view of a sample consumable plate in one embodiment;

[0037] Figure 7 for Figure 6 A top view of the microfluidic detection unit with the sample consumable plate;

[0038] Figure 8 for Figure 6 Side view of the microfluidic detection unit with the sample consumable plate in the center.

[0039] Description of reference numerals:

[0040] 10. Particle detection device; 100. Light source module; 200. Carrying plate; 400. Camera; 500. Y-axis module; 600. X-axis module; 700. Mixing module; 800. Camera module; 110. Light source; 120. First power source; 130. First switching device; 140. Light source shaping system; 20. Sample consumables board; 510. Y-axis motor; 520. Y-axis lead screw; 530. Y-axis slide rail; 540. Y-axis slider; 610. X-axis motor; 620. X-axis lead screw; 630. X-axis slide rail; 640. X-axis slider; 201. Microfluidic detection unit; 21. Substrate; 22. Sample injection device; 23. Blowing and suction device; 24. First microfluidic device Flow channel; 25, second microchannel; 26, detection window; 28, suction device; 221, injection port; 222, first outlet; 231, first mixing port; 223, second mixing port; 29, connecting channel; 281, filter; 291, moisture-sensitive detection element; 710, third power source; 720, vacuum pump; 282, first sealing plug; 730, first mounting member; 740, first driving member; 101, mixing module; 102, fourth power source; 103, blower and suction pump; 232, second sealing plug; 104, second mounting member; 105, second driving member; 810, objective lens; 820, second power source; 830, second switching device; 840, tube mirror. DETAILED DESCRIPTION

[0041] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0042] See also Figures 1 to 5 In one embodiment, a particle detection device 10 includes a light source module 100, a carrier plate 200, a mixing module 101, and a camera 400 module. The light source module 100 includes at least one light source 110 for irradiating a sample. The carrier plate 200 is used to support a sample consumable plate 20, and the carrier plate 200 is movable. The mixing module 101 is used to mix the sample injected into the sample consumable plate 20. The camera module 800 includes an objective lens 810, and the carrier plate 200 is located between the light source module 100 and the objective lens 810.

[0043] The sample consumable plate 20 is placed on the carrier plate 200, and the sample to be tested is injected into the sample consumable plate 20. The mixing module 101 blows and mixes the sample injected into the sample consumable plate 20. The light source module 100 provides light, which is focused on the sample consumable plate 20. The light passes through the objective lens 810 and is received by the camera 400. The mixing module 101 blows and mixes the sample to prevent the particles from settling, which can improve the detection accuracy. The camera 400 then takes a photo of the sample consumable plate 20, and the entire process is automated. The particle detection device 10 is not only simple to operate, but also can prevent particle sedimentation and improve detection accuracy.

[0044] In one embodiment, the particle detection device 10 further includes an X-axis module 600 and a Y-axis module 500 . The X-axis module 600 and the Y-axis module 500 move along the carrier plate 200 in the X-axis or Y-axis direction.

[0045] Furthermore, the particle detection device 10 also includes a support frame (not shown). The support frame primarily provides support for the light source module 100, the carrier plate 200, the camera module 800, the camera 400, the Y-axis module 500, the X-axis module 600, and the mixing module 700. The support frame can be an integrally formed structure or comprise multiple separate support frame sections, each supporting a respective module.

[0046] The light source module 100 is mounted on the support frame. The light source module 100 includes at least one light source 110 for illuminating the sample. The light source module 100 primarily provides light, causing the stained sample to emit fluorescence. Some biological particles do not require dyeing, and the light source module 100 then provides light for the sample to be tested. Specifically, in this embodiment, the light source module 100 includes at least two light sources 110, a first power source 120, a first switching device 130, and a light source shaping system 140. The at least two light sources 110 are spaced apart on the first switching device 130. The first power source 120 is used to drive the first switching device 130 to rotate relative to the objective lens 810. The light source shaping system 140 is located above the light sources 110. The first power source 120 can be a drive motor, and the first switching device 130 is driven by the drive motor to rotate, enabling precise adjustment to a specific light source 110 for ease of operation. Optionally, the first switching device 130 is a first rotating wheel. In other embodiments, the first switching device 130 can also have other shapes or structures.

[0047] Specifically, the number of light sources 110 is at least three, namely a blue light excitation light source 110, a green light excitation light source 110 and a white light source 110. Among them, the blue light excitation light source 110 and the green light excitation light source 110 are LED point light sources 110, and the white light source 110 is a white light source 110 with a full band for bright field and dark field. Of course, in other embodiments, the number of light sources 110 is not limited to three. For example, the number of light sources 110 can also be two, four, five, and so on. The number of light sources 110 can be set according to specific needs. The number of light source shaping systems 140 corresponds to the number of light sources 110, and the light emitted by the light source 110 is shaped and then emitted to the sample to be detected.

[0048] Furthermore, the light source 110 also includes a circuit board, which is fixedly mounted to the side of the first switching device 130 facing the light source shaping system 140 via fasteners. For example, the circuit board is fixedly mounted to the side of the first switching device 130 facing the light source shaping system 140 via screws. Each light source 110 corresponds to one circuit board, and the light source 110 is electrically connected to the circuit board.

[0049] Furthermore, in this embodiment, the light source shaping system 140 is mounted on the first switching device 130 along with the light source 110, and rotates automatically in conjunction with the drive motor, achieving automated operation. Specifically, the light source shaping system 140 is located between the first switching device 130 and the sample to be tested. The light source shaping system 140 includes a collimating lens and a condenser, which are used to focus the light generated by the light source 110 onto the sample to be tested. Through the action of the collimating lens and the condenser, as much light as possible from the light source 110 is collected, achieving bright illumination.

[0050] Furthermore, the light source shaping system 140 also includes a light homogenizer, which is used to homogenize the light from the light source 110. Through the light homogenizer, the light emitted by the light source 110 is evenly irradiated on the sample to be tested, so that the uniformity of the light irradiated on the sample to be tested reaches more than 85%.

[0051] Furthermore, the light source shaping system 140 has a magnification of 12 times. The light source shaping system 140 magnifies the light emitting area of ​​the light source 110 at the sample position by 12 times to meet the imaging area of ​​the objective lens 810. After the magnification is 12 times, the sample position can fill the field of view of the objective lens 810.

[0052] Furthermore, a carrier plate 200 is mounted on the support frame and is used to carry the sample consumable plate 20 . The carrier plate 200 can be moved above the light source module 100 , so that the sample consumable plate 20 placed on the carrier plate 200 can be moved above the light source module 100 .

[0053] In this embodiment, the carrier plate 200 is indirectly mounted on the support frame. Specifically, the carrier plate 200 is mounted on the Y-axis module 500, and the carrier plate 200 moves relative to the objective lens 810 via the Y-axis module 500. The X-axis module 600 is mounted on the support frame, and the Y-axis module 500 and the carrier plate 200 move relative to the objective lens 810 via the X-axis module 600. Therefore, the X-axis module 600 can drive the Y-axis module 500 and the carrier plate 200 to move back and forth horizontally, while the Y-axis module 500 can drive the carrier plate 200 to move back and forth longitudinally.

[0054] Specifically, the Y-axis module 500 includes a Y-axis motor 510, a Y-axis screw rod 520, a Y-axis slide rail 530 and a Y-axis slider 540. The supporting plate 200 is installed on the Y-axis slider 540. The Y-axis slider 540 can slide along the Y-axis slide rail 530. The Y-axis slider 540 is screwed on the Y-axis screw rod 520. The Y-axis motor 510 drives the Y-axis screw rod 520 to rotate, thereby driving the Y-axis slider 540 to move longitudinally along the Y-axis slide rail 530, thereby achieving the purpose of moving the supporting plate 200 in the longitudinal direction.

[0055] The X-axis module 600 includes an X-axis motor 610, an X-axis screw 620, an X-axis slide rail 630, and an X-axis slider 640. The X-axis slider 640 is fixedly mounted on the support frame, and the X-axis slide rail 630 is movable as the X-axis slider 640. The X-axis motor 610 drives the X-axis screw 620 to rotate, and the rotation of the X-axis screw 620 drives the X-axis slide rail 630 to move horizontally relative to the X-axis slider 640, thereby achieving the purpose of moving the carrier plate 200 in the longitudinal direction. Therefore, in this application, the carrier plate 200 can reciprocate in the transverse and longitudinal directions.

[0056] Further, please refer again to Figures 1 to 5The camera module 800 further includes a camera 400, which is located above the objective lens 810. The camera 400 is used to take pictures of the sample consumable plate 20 carried on the carrier plate 200. The camera 400 can also transmit the photographed images to an industrial computer via a data cable for image analysis. Furthermore, the camera module 800 further includes a second power source 820, a second switching device 830, and at least two filters. The at least two filters are spaced apart on the second switching device 830, and the second power source 820 is used to drive the second switching device 830 to rotate relative to the objective lens 810. The second switching device 830 is located between the objective lens 810 and the camera 400. The objective lens 810 is disposed on the support frame, the carrier plate 200 is located between the light source module 100 and the objective lens 810, the at least two filters are spaced apart and disposed on the second switching device 830, and the second power source 820 is used to drive the second switching device 830 to rotate relative to the objective lens 810. The second switching device 830 is located between the objective lens 810 and the camera 400. Optionally, the second switching device 830 is a second rotating wheel. In other embodiments, the second switching device 830 can also have other shapes or structures.

[0057] In this embodiment, the first power source 120 and the second power source 820 are different power sources. That is, the first switching device 130 and the second switching device 830 are driven by different power sources. For example, the power source can be a drive motor, and the first switching device 130 and the second switching device 830 are driven by different drive motors, respectively, which can automatically and accurately adjust to the dedicated light source 110 and filter, facilitating operation. Of course, in other embodiments, the first power source 120 and the second power source 820 can also be the same power source. That is, the first switching device 130 and the second switching device 830 are driven to rotate by the same power source.

[0058] Furthermore, the at least two filters include at least a blue light filter and a green light filter. The blue light filter corresponds to the blue light excitation light source 110, and the green light filter corresponds to the green light excitation light source 110. The white light source 110 does not require a filter, so no filter is provided corresponding to the white light source 110. Of course, in other embodiments, the number of filters can also be specifically set according to the number of light sources 110.

[0059] Furthermore, the second switching device 830 is provided with a mounting hole, into which the optical filter is mounted. That is, the optical filter is embedded in the second switching device 830. The second switching device 830 is also provided with a first fastening ring and a second fastening ring, located on opposite sides of the optical filter, for fastening the optical filter to the mounting hole of the second switching device 830.

[0060] Furthermore, the camera module 800 further includes a tube lens 840, which is located between the second switching device 830 and the camera 400. The tube lens 840 is used to change the magnification of the objective lens 810, so as to make the image captured by the camera 400 clearer and improve the counting accuracy.

[0061] Furthermore, the particle detection device 10 further includes a mixing module 700 , which is used to mix or dye the sample injected into the sample consumable plate 20 .

[0062] It should be noted that this embodiment is described using biological cells as an example, so the particle detection device 10 of this embodiment includes a mixing module 700. Of course, in other embodiments, the particles can also be other biological particles, and the mixing module 700 can be omitted.

[0063] In this embodiment, the sample consumable plate 20 includes a plurality of microfluidic detection units 201. For example, please refer to Figure 6 The sample consumable plate 20 includes 24 microfluidic detection units 201. The 24 microfluidic detection units 201 are distributed in 2 rows with 12 in each row, forming a 24-channel sample consumable plate 20. The 24 microfluidic detection units 201 can be tested sequentially to improve work efficiency. Of course, in other embodiments, the sample consumable plate 20 can also include other numbers of microfluidic detection units 201, such as 2, 3, 10, 20, etc. The number of microfluidic detection units 201 can be set according to actual needs.

[0064] The microfluidic unit included in the sample consumable plate 20 can be arranged in a horizontal arrangement or a vertical arrangement; the corresponding consumable plate can be marked so that the corresponding microfluidic unit can be identified more quickly.

[0065] See also Figure 7 and Figure 8The microfluidic detection unit 201 of the sample consumable plate 20 includes a substrate 21, a sampling device 22 and a suction device 28. The sampling device 22 and the suction device 28 are arranged on the substrate 21. The substrate 21 is embedded with a flow channel. The suction device 28 is connected to the sampling device 22 through the flow channel. The mixing module 700 is connected to the suction device 28 and sucks the suction device 28 so that the sample in the sampling device 22 enters the flow channel for staining. The substrate 21 can be made of polystyrene (PS), polycarbonate (PC) or polymethyl methacrylate (PMMA). The substrates 21 of all the microfluidic detection units 201 on the sample consumable plate 20 are integrally formed.

[0066] Optionally, the flow channel includes a first microchannel 24 and a second microchannel 25. The first microchannel 24 and the second microchannel 25 are not directly connected. Figure 7 、 8 In the embodiment, the second microchannel 25 is located below the first microchannel 24 , and the first microchannel 24 and the second microchannel 25 form a double-layer channel structure.

[0067] In one embodiment, the sampling device 22 is provided on the substrate 21, and the top of the sampling device 22 is provided with a sampling port 221. Specifically, the top of the sampling device 22 is opened to form the sampling port 221, which improves the convenience of sampling. Of course, in other embodiments, the sampling port 221 can also be opened at the top of the sampling device 22, and the size of the sampling port 221 is smaller than the inner diameter of the top of the sampling device 22. The bottom of the sampling device 22 is provided with a first outlet 222. Specifically, the first outlet 222 is opened on the bottom surface of the sampling device 22. Of course, in other embodiments, the first outlet 222 can also be opened on the side of the bottom of the sampling device 22.

[0068] The second microchannel 25 is embedded in the substrate 21. One end of the second microchannel 25 is connected to the sample injection device 22 via the first outlet 222. A dye is embedded in the second microchannel 25. Specifically, the diameter of the second microchannel 25 can range from 10 microns to 1000 microns. The first microchannel 24 can be formed on the substrate 21 using microfluidic technology, thereby being embedded in the substrate 21 to prevent the second microchannel 25 from being exposed and damaged. The dye in the second microchannel 25 is sprayed into the second microchannel 25, and the dye is used to dye the sample.

[0069] The second microchannel 25 is bent to extend the dyeing path, thereby improving the dyeing effect. Figure 7In the embodiment, the second microchannel 25 includes a plurality of straight segments, each of which is bent and connected to form the second microchannel 25. Of course, in other embodiments, the second microchannel 25 can also be an arc segment. The second microchannel 25 is not directly connected to the first microchannel 24. For example, Figure 8 In the embodiment, the second microchannel 25 is located below the first microchannel 24 , and the second microchannel 25 and the first microchannel 24 form a double-layer channel structure.

[0070] Furthermore, a detection window 26 is provided on the substrate 21, and one end of the detection window 26 is connected to the second microchannel 25. The detection window 26 is mainly used for the camera 400 to take pictures, so the detection window 26 is transparent.

[0071] The suction device 28 is provided on the substrate 21, and the other end of the detection window 26 is connected to the suction device 28. For example, the other end of the detection window 26 is connected to the suction device 28 via a connecting channel 29. The diameter of the connecting channel 29 can also range from 10 microns to 1000 microns. A filter 281 is provided in the suction device 28, and a hydrophobic and breathable material is provided in the filter 281. When the liquid in the second microchannel 25 reaches the filter 281, the filter 281 keeps the liquid in the entire second microchannel 25 in a non-flowing state or a slowly flowing state, ensuring that the liquid in the detection window 26 no longer flows or flows slowly, which is convenient for observation.

[0072] Furthermore, a moisture-sensitive detection element 291 is provided in the connecting channel 29 . When the liquid in the second microchannel 25 passes through the moisture-sensitive detection element 291 , the moisture-sensitive detection element 291 changes color, proving that the microchannel detection unit 201 has been used.

[0073] In one embodiment, the mixing module 700 includes a third power source 710, an air extractor 720, and a first needle (not shown). The first needle is connected to the air extractor 720 and inserted into the suction device 28. For example, a first sealing plug 282 is provided at the top of the suction device 28 for inserting the first needle. The first needle is a steel needle. The first sealing plug 282 can be a silicone plunger or a soft sealing rubber to facilitate the insertion of the first needle to drain the suction device 28.

[0074] The third power source 710 is used to drive the vacuum pump 720 to operate, thereby evacuating the suction device 28 on the sample consumable plate 20 through the first needle, thereby allowing the sample in the injection device 22 of the sample consumable plate 20 to be stained through the second microfluidic channel 25 and flow into the detection window 26. The first needle does not come into contact with the liquid in the suction device 28, and the first needle will not be contaminated, and no cleaning is required, thereby reducing costs.

[0075] The sample is added to the injection device 22 through the injection port 221, and the first needle of the mixing module 700 is inserted into the suction device 28. The third power source 710 is used to drive the vacuum pump 720 to extract air from the suction device 28 through the first needle. The sample in the injection device 22 is drawn into the second microchannel 25 from the first outlet 222. Since the second microchannel 25 is embedded with a dye, the sample is dyed in the second microchannel 25 and flows into the detection window 26 under the suction action of the vacuum pump 720, and finally reaches the filter 281. The filter 281 is provided with a hydrophobic and breathable material. When the liquid in the second microchannel 25 reaches the filter 281, the filter 281 keeps the liquid in the entire second microchannel 25 in a non-flowing state or a slow-flowing state, ensuring that the liquid in the detection window 26 no longer flows or flows slowly, so as to facilitate observation and counting of the detection window 26 through a microscope.

[0076] Specifically, the third power source 710 can be a first linear stepper motor. The vacuum pump 720 includes a first cylinder and a first piston rod. One end of the first piston rod is disposed within the first cylinder, and the other end of the first piston rod is disposed at the output end of the first linear stepper motor. The first needle is connected to the first cylinder. For example, when the first linear stepper motor rotates forward, the first piston rod extends out of the first cylinder, and the vacuum pump 720 extracts air from the suction device 28. When the first linear stepper motor rotates backward, the first piston rod retracts into the first cylinder, and the vacuum pump 720 stops extracting air from the suction device 28.

[0077] Furthermore, the particle detection device 10 also includes a first mounting member 730 and a first driving member 740. The first mounting member 730 is disposed on the first driving member 740, and the first needle is disposed on the first mounting member 730. The first driving member 740 is configured to drive the first mounting member 730 to move so that the first needle is inserted into the suction device 28 of the sample consumable plate 20. For example, the first driving member 740 is a drive motor, which drives the first mounting member 730 toward the suction device 28 until the first needle is inserted into the suction device 28. After the detection is completed, the drive motor reverses and drives the first mounting member 730 away from the suction device 28, thereby removing the first needle from the suction device 28.

[0078] In one embodiment, the microfluidic detection unit 201 further includes a blowing and suction device 23. The sample injection device 22 and the blowing and suction device 23 are disposed on the substrate 21. A flow channel is embedded in the substrate 21. The blowing and suction device 23 is connected to the sample injection device 22 via the flow channel. The mixing module 101 is connected to the blowing and suction device 23 and blows or draws air from the blowing and suction device 23, so that the sample in the sample injection device 22 flows into the blowing and suction device 23 through the flow channel, or the sample in the blowing and suction device 23 flows into the sample injection device 22 through the flow channel, so that the sample is mixed.

[0079] Specifically, the blowing and suction device 23 is arranged on the substrate 21, and a first mixing port 231 is provided at the bottom of the blowing and suction device 23. Specifically, the first mixing port 231 is arranged on the side of the bottom of the blowing and suction device 23. Of course, in other embodiments, the first mixing port 231 can also be arranged on the bottom surface of the blowing and suction device 23. The bottom of the sampling device 22 is also provided with a second mixing port 223 spaced apart from the first outlet 222. Similarly, the second mixing port 223 is arranged on the side of the bottom of the sampling device 22, and the first mixing port 231 and the second mixing port 223 are arranged opposite each other. One end of the first microchannel 24 is connected to the blowing and suction device 23 through the first mixing port 231, and the other end is connected to the sampling device 22 through the second mixing port 223. The blowing and suction device 23 and the sampling device 22 can be arranged close to each other or at intervals, as long as the blowing and suction device 23 is connected to the sampling device 22 through the first microchannel 24. The blowing and suction device 23 is located between the sample injection device 22 and the suction device 28, which improves the rationality of the layout and saves space.

[0080] Furthermore, the diameter of the first microchannel 24 can range from 10 micrometers to 1000 micrometers. The first microchannel 24 can be formed on the substrate 21 using microchannel technology. For example, the first microchannel 24 can be embedded inside the substrate 21 to prevent the microchannel from being exposed and damaged.

[0081] Furthermore, the mixing module 101 includes a fourth power source 102, a blower 103 and a second needle (not shown), and the second needle is used to be inserted into the blowing and suction device 23. For example, a second sealing plug 232 for inserting the second needle is provided on the top of the blowing and suction device 23. The second sealing plug 232 can be a silicone plunger or a sealing soft glue, which can facilitate the insertion of the second needle to blow or extract air into the blowing and suction device 23. The second needle is connected to the blower 103, and the second needle is used to be inserted into the blowing and suction device 23 on the sample consumable plate 20. The fourth power source 102 is used to drive the blower 103 to move so as to blow or extract air to the blowing and suction device 23 on the sample consumable plate 20 through the second needle, thereby mixing the sample in the sampling device 22 of the sample consumable plate 20. The second needle will not come into contact with the sample in the blowing and suction device 23, will not cause contamination, does not need to be cleaned, and reduces costs.

[0082] Specifically, the fourth power source 102 is a second linear stepper motor, and the blower / suction device 103 includes a second cylinder and a second piston rod. One end of the second piston rod is disposed within the second cylinder, and the other end of the second piston rod is disposed at the output end of the second linear stepper motor. The second needle is connected to the second cylinder. For example, when the second linear stepper motor rotates forward, thereby driving the second piston rod to extend from the second cylinder, the blower / suction device 103 evacuates air from the blower / suction device 23, and the sample in the sample injection device 22 flows into the blower / suction device 23 through the first microchannel 24. When the second linear stepper motor rotates backward, thereby driving the second piston rod to retract into the second cylinder, the blower / suction device 103 blows air into the blower / suction device 23, and the sample in the blower / suction device 23 flows into the sample injection device 22 through the first microchannel 24. The second linear stepper motor is rotated forward and reverse to drive the second piston rod to extend or retract the second cylinder body, thereby achieving the purpose of vacuuming or blowing the suction device 23, so that the sample injected into the sample tube is blown back and forth to mix, prevent sample precipitation, ensure the cell concentration of the test, and improve the accuracy of the test.

[0083] Furthermore, the particle detection device 10 also includes a second mounting member 104 and a second driving member 105. The second mounting member 104 is mounted on the second driving member 105, and the second needle is mounted on the second mounting member 104. The second driving member 105 is used to drive the second mounting member 104 to move so that the second needle is inserted into the blowing and suction device 23 of the sample consumable plate 20. For example, the second driving member 105 is a drive motor, which drives the second mounting member 104 toward the blowing and suction device 23 until the second needle is inserted into the blowing and suction device 23. After the detection is completed, the drive motor reverses and drives the second mounting member 104 away from the blowing and suction device 23, thereby removing the second needle from the blowing and suction device 23.

[0084] The specific working principle of the particle detection device 10 is as follows:

[0085] The sample consumable plate 20 is placed on the carrier plate 200, and the sample is injected into the sample injection device 22 of the sample consumable plate 20 through the injection port 221. The Y-axis motor 510 then drives the Y-axis screw 520 to rotate, which in turn drives the Y-axis slider 540 to move longitudinally along the Y-axis guide rail 530, allowing the sample consumable plate 20 to be delivered into the inspection chamber. The X-axis motor 610 is activated to move the sample consumable plate 20 horizontally into the field of view of the QR code scanner. After the scan is completed, the X-axis motor 610 moves the sample consumable plate 20 to below the second needle.

[0086] To ensure the correct cell concentration, the sample must be mixed before staining to prevent cell sedimentation. The second driver 105 lowers the second mounting member 104, inserting the second needle into the blowing and suction device 23. The first driver 740 lowers the first mounting member 730, inserting the first needle into the suction device 28.

[0087] The second linear stepper motor rotates forward, driving the second piston rod to extend from the second cylinder. The blower-aspirator 103 then pumps air into the blower-aspirator 23 via the second needle, allowing the sample in the sample injection device 22 to flow into the blower-aspirator 23 through the first microchannel 24. The second linear stepper motor then rotates backward, driving the second piston rod to retract into the second cylinder. The blower-aspirator 103 then pumps air into the blower-aspirator 23 via the second needle, allowing the sample in the blower-aspirator 23 to flow into the sample injection device 22 through the first microchannel 24. At least three cycles of pumping and aspirating are performed to ensure uniform sample mixing.

[0088] After the sample is mixed, the staining operation begins, and the mixing module 700 stains the sample injected into the sample consumable plate 20. Specifically, the first linear stepper motor rotates forward, driving the first piston rod to extend out of the first cylinder. At this point, the vacuum pump 720 draws air from the suction device 28 through the first needle. The sample is drawn into the second microchannel 25 through the first outlet 222. As the sample flows through the second microchannel 25, it is stained and ultimately flows into the detection window 26, ultimately reaching the filter 281. Once the liquid in the second microchannel 25 reaches the filter 281, it is forced through the filter 281 to remain stationary or flow slowly throughout the entire second microchannel 25, ensuring that the liquid in the detection window 26 remains stationary or flows slowly, facilitating observation.

[0089] The light source 110 and filter are selected based on the desired imaging. The first switching device 130 positions the desired light source 110 opposite the objective lens 810, and the second switching device 830 positions the desired filter opposite the camera 400 and the objective lens 810. The light source 110 is turned on, and the light source 110 of the light source module 100 provides light. The light emitted by the light source 110 passes through the light source shaping system 140, which focuses the light on the sample to be measured, thereby stimulating fluorescence. The fluorescence passes through the objective lens 810 and is received by the camera 400.

[0090] Therefore, there is no need for manual staining by the user, and there is no need to stain the sample in advance. The sample to be tested can be directly injected into the sample tube of the sample consumable plate 20 and stained by the mixing module 700. After the sample to be tested is directly injected into the sample consumable plate 20, it is stained by the mixing module 700, and then the camera 400 takes a photo of the sample consumable plate 20. The whole process is automated, which can not only improve efficiency but also improve accuracy. The amount of sample injection has no effect on the accuracy of the result. The dye pre-embedded in the second microfluidic channel 25 eliminates the step of manually preparing the dye, ensures the consistency of the staining concentration, and improves accuracy. The sampling amount is precisely controlled by the third power source 710 and the vacuum pump 720 to ensure that the detection process and results are more accurate and not interfered with by manual operation. Moreover, before the staining operation, the sample is mixed by blowing and inhaling to prevent cell sedimentation, ensure the cell concentration of the test, and improve the accuracy of the test.

[0091] Since the first switching device 130 can be rotated to select the corresponding light source 110 according to the required imaging requirements, and the second switching device 830 can be rotated to select the corresponding filter, each fluorescence corresponds to a dedicated light source 110 and filter, which can effectively avoid crosstalk between different light sources 110 and eliminate the need for a dichroic mirror. It is suitable for the mixed use of multiple fluorescent substances, reduces the difficulty of filter production, and reduces costs.

[0092] In other embodiments, the particle detection device 10 may not be provided with a mixing module 700, and the sample consumable plate 20 may not be provided with a suction device 28. The sample consumable plate 20 includes a substrate 21, a sampling device 22 and a blowing and suction device 23. The sampling device 22 and the blowing and suction device 23 are arranged on the substrate 21, and a flow channel is embedded in the substrate 21. The blowing and suction device 23 is connected to the sampling device 22 through the flow channel. The mixing module 101 and the blowing and suction device 23 are used to mix or dye the sample in the particle detection unit. Specifically, the mixing module 101 is connected to the blowing and suction device 23 and blows or extracts air from the blowing and suction device 23, so that the sample in the sampling device 22 flows through the flow channel to the blowing and suction device 23, or the sample in the blowing and suction device 23 flows through the flow channel to the sampling device 22, so that the sample is mixed. Optionally, the flow channel has at least two branches, and the two branches are connected and disconnected with the suction device 28 through valve control. The valve of one of the branches is opened, and the mixing module 101 draws air or blows air, and the sample flows back and forth between the blowing and suction device 23 and the sample injection device 22 through the branch to achieve uniform mixing; after mixing, the branch is closed, and the valve of the other branch is opened, so that the color substance can be buried in the other branch, and the mixed sample is drawn air through the mixing module 101, enters the other branch, is dyed, and flows to the detection window 26 for detection. Alternatively, the flow channel is one, and the mixing module 101 draws air, so that the sample flows between the blowing and suction device 23 and the sample injection device 22 through the flow channel to achieve uniform mixing or dyeing. Optionally, in one embodiment, the flow channel can allow at least a portion of the liquid analyte to flow in the channel, and at least a portion of the flow channel has a bend or obstruction, so that the liquid analyte is used to adjust the flow rate of the liquid analyte at the bend or obstruction to extend the flow path.

[0093] Optionally, the particle detection unit further includes a color substance, which is disposed in the flow channel, or in the sample injection device 22, or in the mixing device, or in the mixing module 101, and is used to mix with the liquid analyte injected into the sample injection device 22. The sample is a liquid analyte.

[0094] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0095] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

[0096] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0097] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0098] In the present invention, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediate medium; internal communication between two components, or interaction between two components, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0099] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0100] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

Claims

1. A particle detection device, characterized in that: The particle detection device comprises: a light source module, comprising at least one light source for illuminating a sample; A carrying plate, the carrying plate is used to carry the sample consumable plate, and the carrying plate is movable; A mixing module, used to mix the sample injected into the sample consumable plate; and A camera module includes an objective lens, wherein the carrier plate is located between the light source module and the objective lens; The sample consumable plate includes a base plate, a sample injection device and a blowing and suction device, wherein the sample injection device and the blowing and suction device are arranged on the base plate, and a flow channel is embedded in the base plate; Wherein, the flow channel has at least two branches, and the two branches are connected and disconnected with the blowing and suction device through valve control; when the valve of one branch is opened, the mixing module blows or draws air to the blowing and suction device, so that the sample in the injection device flows between the blowing and suction device and the injection device through the branch to mix the sample; when the valve of the other branch embedded with dye is opened, the mixing module draws air to the blowing and suction device, so that the mixed sample enters the other branch to be dyed and flows to the detection window for detection; or, The particle detection device also includes a mixing module, and the sample consumable plate also includes a suction device; the flow channel includes a first microchannel and a second microchannel; a dye is embedded in the second microchannel; the blowing and suction device is connected to the sampling device through the first microchannel, and the mixing module is connected to the blowing and suction device and blows or draws air to the blowing and suction device, so that the sample in the sampling device flows through the first microchannel into the blowing and suction device or the sample in the blowing and suction device flows through the first microchannel into the sampling device, so that the sample is mixed; the suction device is connected to the sampling device through the second microchannel, and the mixing module is connected to the suction device and sucks the suction device, so that the sample in the sampling device enters the second microchannel for dyeing and flows into the detection window.

2. The particle detection device according to claim 1, characterized in that: The light source module includes at least two light sources, a first power source, a first switching device and a light source shaping system. The at least two light sources are arranged at intervals on the first switching device. The first power source is used to drive the first switching device to rotate relative to the objective lens. The light source shaping system is located above the light source.

3. The particle detection device according to claim 1, characterized in that: It also includes an X-axis module and a Y-axis module, and the X-axis module and the Y-axis module move along the carrier plate in the X-axis or Y-axis direction.

4. The particle detection device according to claim 3, characterized in that: The carrying plate may also be disposed on the Y-axis module, and the carrying plate moves relative to the objective lens via the Y-axis module.

5. The particle detection device according to claim 3, characterized in that: The X-axis module may be arranged on a supporting frame, and the Y-axis module and the carrying plate move relative to the objective lens via the X-axis module.

6. The particle detection device according to claim 1, characterized in that: The camera module further includes a camera, which is located above the objective lens and is used to take pictures of the sample consumable plate carried on the carrying plate.

7. The particle detection device according to claim 6, characterized in that: The photographic module also includes a second power source, a second switching device and at least two filters. The at least two filters are arranged on the second switching device at intervals. The second power source is used to drive the second switching device to rotate relative to the objective lens. The second switching device is located between the objective lens and the camera.

8. The particle detection device according to claim 7, characterized in that: The photographic module further includes a tube lens, and the tube lens is located between the second switching device and the camera.

9. The particle detection device according to claim 1, characterized in that: The mixing module includes a third power source, an exhaust pump and a first needle. The first needle is connected to the exhaust pump. The third power source is used to drive the exhaust pump to extract air from the suction device of the sample consumable plate through the first needle, thereby allowing the sample in the sampling device of the sample consumable plate to pass through the flow channel for staining and flow into the detection window.

10. The particle detection device according to claim 9, characterized in that: The third power source is a first linear stepper motor, and the vacuum pump includes a first cylinder body and a first piston rod. One end of the first piston rod is arranged in the first cylinder body, and the other end of the first piston rod is arranged at the output end of the first linear stepper motor. The first needle is connected to the first cylinder body.

11. The particle detection device according to claim 10, characterized in that: It also includes a first mounting member and a first driving member, the first mounting member is arranged on the first driving member, the first needle is arranged on the first mounting member, and the first driving member is used to drive the first mounting member to move so that the first needle is inserted into the vacuum device of the sample consumable plate.

12. The particle detection device according to any one of claims 1 to 11, characterized in that: The mixing module includes a fourth power source, a blower and a second needle. The second needle is connected to the blower. The second needle is used to be inserted into the blowing and suction device on the sample consumable plate. The fourth power source is used to drive the blower to blow or draw air from the blowing and suction device of the sample consumable plate through the second needle, thereby mixing the sample in the sampling device of the sample consumable plate.

13. The particle detection device according to claim 12, characterized in that: The blowing and suction power source is a second linear stepping motor. The blower includes a second cylinder and a second piston rod. One end of the second piston rod is arranged in the second cylinder, and the other end of the second piston rod is arranged at the output end of the second linear stepping motor; the second needle is connected to the second cylinder.

14. The particle detection device according to claim 12, characterized in that: It also includes a second mounting member and a second driving member, the second mounting member is arranged on the second driving member, the second needle is arranged on the second mounting member, and the second driving member is used to drive the second mounting member to move so that the second needle is inserted into the blowing and suction device of the sample consumable plate.

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