A device for acclimatizing and cultivating salt-tolerant bacteria in saline wastewater and a method for using the device
By designing a device containing acclimation zone and a culture zone, using multi-zone environment and sustained-release fillers, the problem of insufficient domestication and adaptability of microorganisms in salt-containing wastewater is solved, and efficient sewage treatment and stable operation of the system is achieved.
Patent Information
- Application Number
- CN202111420668.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-11-26
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2041-11-26
AI Technical Summary
During the salt-containing wastewater treatment process, high concentrations of salt substances have an inhibitory effect on microorganisms, resulting in poor biological treatment effects, and fluctuations in water quality affect the activity of bacterial species, making it difficult to maintain the stable operation of the system.
A device containing a domestication area and a culture area is designed. The domestication area includes anaerobic/hypoxia zone, an aerobic zone and an oxygen zone. The culture area includes anaerobic, aerobic and hypoxia culture areas that are not connected to each other. By controlling the water quality environment and using sustained release fillers, rapid domestication and adaptability of microorganisms can be achieved.
By accelerating the domestication progress of bacterial species and improving adaptability, the sewage treatment efficiency is enhanced, the operating costs of the system are reduced, and better adaptability to water quality fluctuations is achieved.
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Figure CN114031182B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of sewage treatment, and specifically relates to a device for acclimatizing and cultivating salt-tolerant bacteria in saline wastewater and a use method thereof. Background Art
[0002] In the production process of chemical, pharmaceutical and fuel, the wastewater produced contains high concentration of organic matter and salt substances. When the physicochemical method is used to treat this kind of wastewater, it is found that the method has large investment and high operating costs, and it is difficult to achieve the expected purification effect. When the biological method is used to treat this kind of wastewater, the effect is not good because the high concentration of salt substances has an inhibitory effect on microorganisms; therefore, the domestication and screening of salt-tolerant bacteria or halophilic bacteria suitable for salt-containing wastewater is the core issue of the biochemical treatment of this kind of sewage.
[0003] However, the successfully domesticated and screened salt-tolerant microorganisms all belong to sensitive bacteria. When the salinity of the influent salinity of saline wastewater fluctuates, it will greatly affect the activity of the bacteria. Therefore, during the operation of saline wastewater, the salinity of the water body needs to be maintained relatively stable. However, due to various reasons, the influent salinity of many projects in actual engineering varies greatly, which makes it difficult for the biochemical system of saline wastewater to operate stably.
[0004] Currently, there are two main solutions for the biological treatment of high-salt wastewater: one is to cultivate activated sludge suitable for specific water quality through long-term domestication of activated sludge in the treatment process, and then carry out biological treatment. However, this sludge cultivation method has a long cultivation cycle and general adaptability to water quality fluctuations; the other is to add exogenous salt-tolerant microorganisms during the treatment process. This method also has the function of maintaining the stable operation of the biochemical stage, but this method requires long-term addition of bacteria for a certain period of time. The exogenously added salt-tolerant microorganisms may not be suitable for the adaptability of the corresponding sewage, the domestication and screening cycle is relatively long, and it also requires a certain amount of adaptation time to the fluctuations in water quality. Summary of the invention
[0005] The first technical problem to be solved by the present invention is:
[0006] A device for acclimating and culturing salt-tolerant bacteria in saline wastewater is provided. A device for acclimating and culturing salt-tolerant bacteria in saline wastewater is separately set outside a sewage treatment pool (sewage plant), that is, a device for acclimating and culturing synchronous strains is separately set to accelerate the acclimation progress of strains. The influent water quality of the device is the same as that of the sewage treatment pool, ensuring the adaptability of the acclimated strains to actual sewage.
[0007] The second technical problem to be solved by the present invention is:
[0008] Provided is a method for using the device for acclimatizing and culturing salt-tolerant bacteria in saline wastewater.
[0009] In order to solve the first technical problem, the technical solution adopted by the present invention is:
[0010] A device for acclimatizing and culturing salt-tolerant bacteria in saline wastewater:
[0011] Includes connected acclimatization and cultivation areas;
[0012] The acclimatization zone includes an anaerobic / anoxic zone, an aerobic zone and an anoxic zone which are connected in sequence;
[0013] The culture area includes an anaerobic culture area, an aerobic culture area and an anoxic culture area which are not connected to each other;
[0014] The aerobic zone is provided with a first treatment zone and a second treatment zone connected in parallel;
[0015] The anaerobic / anoxic zone is connected to a water inlet pipe, and the anaerobic / anoxic zone, the aerobic zone and the anoxic zone are connected to a drainage pipe, so that during operation, the bacterial species in each area of the acclimation zone can flow out separately and continuously to the sewage treatment plant connected to the device, specifically to the corresponding anaerobic zone, aerobic zone and anoxic zone in the sewage treatment plant, so as to improve the treatment efficiency of each section of the sewage treatment plant.
[0016] The first treatment area and the second treatment area are respectively provided with a first slow-release filler;
[0017] A second slow-release filler is arranged in the anoxic zone.
[0018] A separate culture area is set up outside the acclimation area to expand the bacterial strains and speed up the acclimation of the bacterial strains in the device. The water quality of the inlet water in the acclimation area and the acclimation area is the same, which ensures the adaptability of the acclimated bacterial strains. Liquid culture medium is added to the acclimation area, and the water in the liquid culture medium is used as the inlet water in the acclimation area. The successfully cultivated bacterial strains flow into the acclimation area, which can speed up the renewal of microorganisms in the acclimation area, so that the most suitable microorganisms quickly become dominant strains, ensuring the sewage treatment efficiency.
[0019] The anoxic zone is arranged after the aerobic zone, so that the denitrifying bacteria can fully utilize the nitrate nitrogen produced during the nitrification process in the aerobic zone.
[0020] Connecting part of the water inlet to the upper part of the anoxic zone can effectively utilize the carbon source in the raw water. Driven by the hydraulic force and bubbles of the bottom and upper water inlets, the cultivation and domestication of anoxic denitrifying bacteria can be achieved without setting up stirring. Under certain conditions, the enrichment and domestication of anaerobic ammonia-oxidizing bacteria can also be achieved.
[0021] According to one embodiment of the present invention, an anaerobic / anoxic zone outlet pipe and a nitrification liquid return pipe are provided in the anaerobic / anoxic zone.
[0022] The nitrification liquid reflux pipe is provided with a valve to control the reflux amount of the nitrification liquid, so that the microenvironment of the water body in the anaerobic / anoxic zone can be controlled as needed to achieve the domestication of the anaerobic microbial flora or the anoxic microbial flora, and realize the dual-purpose function of one zone without the need for an additional reflux pump.
[0023] That is, the anaerobic / anoxic zone can be freely switched to an anaerobic environment or anoxic environment as needed by controlling the opening and closing of the valve of the nitrification liquid return pipe. That is, when the valve is opened for return flow, the anaerobic / anoxic zone is anoxic zone, and when the return flow is closed, the anaerobic / anoxic zone is an anaerobic zone.
[0024] According to one embodiment of the present invention, the anaerobic / anoxic zone is used to domesticate polyphosphate-accumulating bacteria, denitrifying bacteria and other bacteria that grow in an anaerobic / anoxic environment.
[0025] According to one embodiment of the present invention, the aerobic zone is connected to the anoxic zone through an aerobic zone outlet pipe. Under hydraulic drive, the sewage in the aerobic zone carries part of the dissolved oxygen and flows through the aerobic zone outlet pipe to the anoxic zone, thereby creating an anoxic environment in the water body of the anoxic zone. It is not necessary to add an aeration device or a stirring device in the anoxic zone, so that the environment in the anoxic zone can be adapted to the growth of denitrifying bacteria.
[0026] The anoxic zone is provided with a second slow-release filler for enriching the microbial flora, which provides nutrition for the flora in the anoxic acclimation zone and accelerates the acclimation progress.
[0027] The anoxic zone water inlet pipe is used to flow a portion of sewage into the anoxic zone, provide a carbon source for the anoxic zone flora, and fully utilize nutrients in the raw water.
[0028] According to one embodiment of the present invention, an aeration disk is provided in each of the first treatment area and the second treatment area.
[0029] According to one embodiment of the present invention, an anoxic zone water inlet pipe is provided in the anoxic zone.
[0030] According to one embodiment of the present invention, the first sustained-release filler comprises the following raw materials in parts by weight:
[0031] 1-1.5 parts of beeswax, 2-3 parts of biological wax, 1-2 parts of sodium carbonate, 0.2-0.5 parts of potassium humate, and 0.01-0.06 parts of trace element mixture.
[0032] According to one embodiment of the present invention, the second sustained-release filler comprises the following raw materials in parts by weight:
[0033] 2-3 parts of beeswax, 1-1.5 parts of biological wax, 1-2 parts of polyhydroxyalkanoate, 0.2-1 parts of potassium fulvic acid, and 0.01-0.03 parts of trace element mixture.
[0034] The biological wax was purchased from MEC Company, Australia.
[0035] The trace element mixture includes the following raw materials in parts by weight:
[0036] FeCl 3 6H 2 O 1.5g; H 3 BO 3 0.15g portion; CuSO 4 ·5H 2 O 0.03g part; KI 0.03g part; Na 2 MoO 4 ·2H 2 O 0.06g parts; MnCl 2 ·4H 2 O 0.12g parts; ZnSO 4 7H 2 O 0.12g parts; CoCl 2 ·2H 2 O 0.12g portion.
[0037] Different formulations of bio-wax-based sustained-release activators are developed for aerobic microbial flora and anoxic microbial flora. The formulation ingredients are particularly suitable for the domestication of special flora and loading them onto inert fillers. The activator and microorganisms are loaded into the inert fillers before use, which can ensure that salt-tolerant microorganisms can be quickly started and reproduced rapidly in the high-salt environment during the domestication stage.
[0038] According to one embodiment of the present invention, the first sustained-release filler is loaded in the suspension filler.
[0039] According to one embodiment of the present invention, the second sustained-release filler is loaded in a suspension filler.
[0040] According to one embodiment of the present invention, a first connecting channel and a second connecting channel spanning the second processing zone are provided between the first processing zone and the aerobic culture zone.
[0041] According to one embodiment of the present invention, online dissolved oxygen probes, pH probes and online redox potential analyzer probes (ORP probes) are respectively arranged in the anaerobic / anoxic zone, the aerobic zone and the anoxic zone to assist in the regulation of acclimation parameters.
[0042] According to one embodiment of the present invention, an online spectrophotometer is arranged in the culture area to measure the change of absorbance at 600 nm to assist in the culture of bacterial strains.
[0043] In order to solve the second technical problem, the technical solution adopted by the present invention is:
[0044] A method for acclimating and culturing salt-tolerant bacteria using a salt-tolerant bacteria acclimation and culturing device in saline wastewater:
[0045] Bacteria cultivation: part of the sewage in the acclimation area is discharged into the cultivation area, and after bacterial cultivation, the sewage rich in bacteria in the cultivation area is discharged into the acclimation area;
[0046] Bacterial flora acclimation: The sewage rich in bacterial flora is sequentially passed through the anaerobic / anoxic zone, the aerobic zone and the anoxic zone to acclimate the bacterial flora to the sewage.
[0047] According to one embodiment of the present invention, the bacterial flora culture: after adding corresponding types of liquid culture media in each area of the culture area, part of the sewage in the anaerobic / anoxic zone in the acclimation zone is discharged into the anaerobic or anoxic culture area, part of the sewage in the aerobic zone is discharged into the aerobic culture area, and part of the sewage in the anoxic zone is discharged into the anoxic culture area. After bacterial flora culture, the sewage rich in the bacterial flora in the anaerobic culture area is returned to the anaerobic / anoxic zone, the sewage rich in the bacterial flora in the aerobic culture area is returned to the aerobic zone, and the sewage rich in the anoxic culture area is returned to the anoxic zone; thereby ensuring that the water bodies in the anaerobic / anoxic zone, the aerobic zone, and the anoxic zone always maintain rich bacterial flora, and the bacterial flora is adapted to anaerobic / anoxic, or aerobic, or anoxic.
[0048] According to an embodiment of the present invention, the sewage treatment step further includes the following steps: opening the aeration plates in the first treatment area and the second treatment area at intervals so that aeration flushing is performed in the first treatment area and the second treatment area;
[0049] According to an embodiment of the present invention, the sewage treatment step further includes the following steps: increasing the aeration volume of the aeration disks in the first treatment area and the second treatment area at intervals so that aeration flushing is performed in the first treatment area and the second treatment area;
[0050] During the process of starting / increasing the aeration volume, the mature bacterial flora growing on the biofilm in the current area will be flushed and aerated, so that the bacterial flora in the first treatment area and the second treatment area will always have one group in the rapid growth stage and one group in the near-maturity stage.
[0051] According to one embodiment of the present invention, the interval opening time is 7 days.
[0052] According to one embodiment of the present invention, the duration of the flushing aeration is 1 hour.
[0053] According to one embodiment of the present invention, during the aeration process of the aeration disk, the sewage in the aerobic zone flows back into the anaerobic / anoxic zone from the nitrification liquid return pipe through the hydraulic fluctuations generated by aeration.
[0054] During the flushing process, the aerobic zone can simultaneously flush the anoxic zone, causing its biofilm to fall off, thus achieving the same function as the aerobic zone.
[0055] According to one embodiment of the present invention, a water inlet container is provided, and the container volume is more than twice the volume of the device. Sewage from the rear end of the sewage plant's water inlet grid and the front end of the biochemical pool is pumped into the water inlet container and used as the initial acclimation culture medium.
[0056] In the process of using a salt-tolerant bacteria acclimatization and cultivation device in saline wastewater, water quality is tested at the outlet of each area every day and a water quality change curve is drawn at the corresponding residence time. The water quality indicators include chemical oxygen demand (COD), ammonia nitrogen content and total nitrogen content. At the initial stage of startup, the various effluent indicators will be higher than the inlet indicators. When the inlet and outlet water quality indicators are the same, it can be preliminarily confirmed that the microorganisms have begun to grow. When the average of various effluent indicators is lower than 80% of the inlet indicators, the commissioning period begins. The inlet pipe is adjusted to take the water from the rear end of the sewage plant grid and the front end of the biochemical pool, and the outlet pipe is adjusted to the front end of each area corresponding to the sewage treatment process.
[0057] One of the technical solutions described above has at least one of the following advantages or beneficial effects:
[0058] 1. The process adopted in the acclimation zone is anaerobic / anoxic-aerobic-anoxic. By placing the anoxic zone at the rear, the denitrifying bacteria can make full use of the nitrate nitrogen produced during the nitrification process in the aerobic zone.
[0059] 2. The device does not have a sedimentation tank, a sludge return process, or a stirring device. It has a simple structure, low equipment cost and operating electricity costs, is not easily damaged, and is easy to operate.
[0060] 3. The aerobic zone is divided into two parts. Through the two independent aeration and flushing devices in the aerobic zone, on the one hand, there is always one group of bacteria in the aerobic zone in the rapid growth stage and the other group in the near-maturity stage; on the other hand, the hydraulic drive and load impact formed by the aeration and flushing water flow are used to synchronously flush the anoxic zone, so that the biofilm in the anoxic zone falls off and there is no need to set up a separate flushing device in the anoxic zone; on the other hand, the aeration and flushing water flow is used to realize the reflux of part of the nitrification liquid in the aerobic zone to the anaerobic / anoxic zone. The reflux volume can be controlled by the nitrification liquid reflux pipe. The anaerobic / anoxic zone can be freely switched to an anaerobic environment or anoxic environment as needed to realize the domestication of anaerobic microbial flora or anoxic microbial flora, and the dual-use function of one zone is realized without the need for an additional reflux pump.
[0061] 4. Set up a synchronous strain culture area separately outside the acclimation area to speed up the acclimation progress of the strains. The water quality of the acclimation area and the culture area is consistent, which ensures the adaptability of the acclimated strains. The strains successfully cultivated in the culture area continuously flow into the acclimation area, which can speed up the renewal of microorganisms in the acclimation area, so that the most suitable microorganisms quickly become dominant strains, ensuring the acclimation progress.
[0062] 5. The inert filler loaded with microorganisms and bioactivators has formed a biofilm before use, which reduces the biofilm formation time of traditional biofilm by at least one month. During operation, the bioactivator shortens the generation cycle of microbial flora and improves the domestication efficiency.
[0063] 6. This device can keep the biological unit in the optimal treatment state. It is not only suitable for the biochemical treatment of saline wastewater, but also suitable for the biological treatment units of almost all sewage treatment plants. BRIEF DESCRIPTION OF THE DRAWINGS
[0064] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.
[0065] Figure 1 Schematic diagram of the device for acclimatizing and cultivating salt-tolerant bacteria in saline wastewater in this embodiment.
[0066] Figure 2 This is a schematic diagram of aeration and flushing of the salt-tolerant bacteria acclimatization and cultivation device in saline wastewater in this embodiment.
[0067] Figure 3 This is a schematic diagram of the distribution of slow-release fillers in the salt-tolerant bacteria acclimatization and cultivation device in saline wastewater in this embodiment.
[0068] Figure 4 This is a physical diagram of the partial nitrification-denitrification reaction achieved in the anaerobic zone.
[0069] Figure 5 This is a physical picture of the suspended filler filled with the first slow-release filler in the aerobic zone.
[0070] Figure 6 This is a physical picture of the suspended filler with a second slow-release filler filled in the anoxic zone.
[0071] Figure 7 This is a physical picture of the biofilm growing on the solid culture medium in the aerobic zone without aeration and flushing.
[0072] Figure 8 This is a physical picture of the biofilm growing on the solid culture medium after aeration and flushing in the aerobic zone.
[0073] Fig. 9 This is a physical picture of active microorganisms flowing out of the outlet pipe.
[0074] Fig.10 This is a physical picture of the suspended filler in the aerobic zone during the operation of the device.
[0075] Reference numerals:
[0076] 100-acclimation zone, 200-cultivation zone, 110-anaerobic / anoxic zone, 111-water inlet pipe, 112-anaerobic / anoxic zone outlet pipe, 113-nitrification liquid reflux pipe, 120-aerobic zone, 121-first treatment zone, 122-second treatment zone, 123-aeration plate, 124-first slow-release filler, 125-aerobic zone outlet pipe, 130-anoxic zone, 131-second slow-release filler, 132-drain pipe, 133-anoxic zone inlet pipe, 210-anaerobic cultivation zone, 221-first connecting channel, 222-second connecting channel, 220-aerobic cultivation zone, 230-anoxic cultivation zone. DETAILED DESCRIPTION
[0077] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions.
[0078] The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.
[0079] In the description of the present invention, if there is a description of first, second, third, etc., it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the sequence of the indicated technical features.
[0080] In the description of the present invention, it is necessary to understand that descriptions involving orientation, such as up, down, left, right, etc., the orientations or positional relationships indicated are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0081] In the description of the present invention, it should be noted that, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meaning of the terms in the present invention in combination with the specific content of the technical solution.
[0082] In order to explain the technical content, achieved objectives and effects of the present invention in detail, the following is an explanation in conjunction with the implementation modes.
[0083] Example
[0084] This embodiment relates to a device for acclimatizing and culturing salt-tolerant bacteria in saline wastewater. The schematic diagram of the device is shown in FIG. Figure 1 .
[0085] The device comprises an acclimation area 100 and a culture area 200 connected by a pipeline;
[0086] The acclimation zone 100 includes an anaerobic / anoxic zone 110, an aerobic zone 120 and an anoxic zone 130 which are connected in sequence;
[0087] The culture area 200 includes an anaerobic culture area 210, an aerobic culture area 220 and an anoxic culture area 230 which are not connected to each other;
[0088] The aerobic zone 120 is provided with a first treatment zone 121 and a second treatment zone 122 connected in parallel. The aerobic zone 120 is provided with an aeration flushing device. The schematic diagram of the aerobic zone aeration flushing is shown in FIG. Figure 2 ;
[0089] The anaerobic / anoxic zone 110 is connected to a water inlet pipe 111 at its beginning, and the anaerobic / anoxic zone 110, the aerobic zone 120 and the anoxic zone 130 are connected to a drainage pipe 132;
[0090] The first processing area 121 and the second processing area 122 are respectively provided with a first slow-release filler 124;
[0091] The anoxic zone 130 is provided with a second slow-release filler 131. The schematic diagram of the slow-release filler distribution in the device is as follows: Figure 3 .
[0092] The anaerobic / anoxic zone 110 is provided with an anaerobic / anoxic zone outlet pipe 112 and a nitrification liquid return pipe 113 .
[0093] The nitrification liquid reflux pipe 113 is provided with a valve.
[0094] The above-mentioned anaerobic / anoxic zone 110 is used to domesticate the polyphosphate-accumulating bacteria, denitrifying bacteria and other bacteria that grow in the anaerobic / anoxic environment. The actual picture of the partial nitrification-denitrification reaction achieved in the anaerobic zone is as follows Figure 4 .
[0095] The aerobic zone 120 is connected to the anoxic zone 130 via the aerobic zone outlet pipe 1.2.5.
[0096] A second slow-release filler 131 for enriching microbial flora is disposed in the anoxic zone 130 .
[0097] An aeration plate 123 is disposed in each of the first processing area 121 and the second processing area 122 .
[0098] The anoxic zone 130 is provided with an anoxic zone water inlet pipe 111 .
[0099] The first sustained-release filler 124 includes the following raw materials.
[0100] The second sustained-release filler 131 includes the following raw materials.
[0101] The first slow-release filler 124 is filled in the suspension filler. The actual picture of the suspension filler filled with the first slow-release filler is as shown in FIG. Figure 5 ;
[0102] The second slow-release filler 131 is filled in the suspension filler. The actual picture of the suspension filler filled with the second slow-release filler is as shown in FIG. Figure 6 , then place a solid culture medium near the above-mentioned suspended filler, and place a solid carbon source near the above-mentioned hanging filler.
[0103] The actual picture of the biofilm growing on the solid culture medium without aeration flushing in the aerobic zone is as follows Figure 7 , it is obvious that there is a large amount of biofilm on the solid culture medium.
[0104] The actual picture of the biofilm growing on the solid culture medium after aeration and flushing in the aerobic zone is as follows Figure 8 ,From the figure, we can know that after aeration flushing, the biofilm on the solid culture medium is basically shed off.
[0105] from Fig. 9 It can also be seen from the actual picture of active microorganisms flowing out of the outlet pipe that after the device cultivates and flushes the colonies, the water contains more active microorganisms.
[0106] In addition, the actual picture of the suspended filler in the aerobic zone during the operation of the device can be obtained from Fig.10 Seen in.
[0107] A first connecting channel 221 and a second connecting channel 222 spanning the second processing area 122 are provided between the first processing area 121 and the aerobic culture area 220 .
[0108] The anaerobic / anoxic zone 110, the aerobic zone 120 and the anoxic zone 130 are respectively provided with an online dissolved oxygen probe, a pH probe and an online oxidation-reduction potential analyzer probe (ORP probe) for assisting in the regulation of domestication parameters.
[0109] An online spectrophotometer is arranged in the culture area 200 .
[0110] A water inlet container is set at the front end of the device, and the container volume is more than twice the volume of the device. The sewage from the rear end of the water inlet grid of the sewage plant and the front end of the biochemical pool is pumped into the water inlet container to be used as a source of bacteria and initial acclimation culture medium. The water inlet container is connected to the water inlet pipe 111 of the device.
[0111] This embodiment also relates to a method for using the above-mentioned device for acclimatizing and culturing salt-tolerant bacteria in saline wastewater, comprising the following steps:
[0112] Bacteria cultivation treatment: the sewage from the anaerobic / anoxic zone 110, the aerobic zone 120 and the anoxic zone 130 in the acclimation zone 100 is discharged into the corresponding culturing zone 200, and after bacterial cultivation, the sewage in the culturing zone 200 is discharged into the corresponding area of the acclimation zone 100;
[0113] Bacteria flora domestication: The sewage is sequentially passed through the anaerobic / anoxic zone 110, the aerobic zone 120 and the anoxic zone 130 to domesticate the corresponding bacteria flora.
[0114] The above sewage treatment step further includes the following steps: opening the aeration plates 123 in the first treatment area 121 and the second treatment area 122 for 7 hours at intervals;
[0115] During the startup process, the mature bacterial flora growing on the biofilm in the current area will be flushed and aerated, so that the bacterial flora in the first treatment area 121 and the second treatment area 122 will always have one group in the rapid growth stage and one group in the near-maturity stage.
[0116] The above are only embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent transformations made using the contents of the present invention specification, or directly or indirectly applied in related technical fields, are also included in the patent protection scope of the present invention.
Claims
1. A method for acclimatizing and culturing salt-tolerant bacteria in saline wastewater, Features: A device for acclimating and cultivating salt-tolerant bacteria in saline wastewater is used, comprising an acclimation area (100) and a cultivation area (200) connected to each other; The acclimatization zone (100) includes an anaerobic / anoxic zone (110), an aerobic zone (120) and an anoxic zone (130) which are connected in sequence; The culture area (200) includes an anaerobic culture area (210), an aerobic culture area (220) and an anoxic culture area (230) which are not connected to each other; The aerobic zone (120) is provided with a first treatment zone (121) and a second treatment zone (122) connected in parallel; The anaerobic / anoxic zone (110) is connected to a water inlet pipe (111), and the anaerobic / anoxic zone (110), the aerobic zone (120) and the anoxic zone (130) are connected to a drainage pipe (132); The first treatment area (121) and the second treatment area (122) are respectively provided with a first slow-release filler (124); A second slow-release filler (131) is provided in the anoxic zone (130); The anaerobic / anoxic zone (110) is provided with an anaerobic / anoxic zone outlet pipe (112) and a nitrification liquid return pipe (113). The nitrification liquid return pipe (113) is provided with a valve. When the valve is opened for return flow, the anaerobic / anoxic zone (110) becomes an anoxic zone (130). When the return flow is closed, the anaerobic / anoxic zone (110) becomes an anaerobic zone. An aeration plate (123) is provided in each of the first treatment area (121) and the second treatment area (122); The first slow-release filler (124) is filled in the suspended filler; the second slow-release filler (131) is filled in the suspended filler; The method for acclimatizing and culturing salt-tolerant bacteria in saline wastewater comprises the following steps: Bacteria cultivation treatment: the sewage in the acclimation area (100) is discharged into the cultivation area (200), and after bacterial cultivation, the sewage rich in bacteria in the cultivation area (200) is discharged into the acclimation area (100); Bacteria domestication: The sewage rich in bacteria is sequentially passed through the anaerobic / anoxic zone (110), the aerobic zone (120) and the anoxic zone (130) to perform bacteria domestication; The bacterial strain domestication step also includes the following steps: the first treatment area (121) and the second treatment area (122) are aerated and flushed at intervals; during the opening process, the mature bacterial community growing on the biofilm in the current area will be flushed and aerated, so that the bacterial communities in the first treatment area (121) and the second treatment area (122) always have one group in the rapid growth stage and one group in the near-maturity stage.
2. The method for acclimating and culturing salt-tolerant bacteria in saline wastewater according to claim 1, Features: The aerobic zone (120) is connected to the anoxic zone (130) via an aerobic zone outlet pipe (125).
3. The method for acclimating and culturing salt-tolerant bacteria in saline wastewater according to claim 1, Features: An anoxic zone water inlet pipe (133) is provided in the anoxic zone (130).
4. The method for acclimating and culturing salt-tolerant bacteria in saline wastewater according to claim 1, Features: A first connecting channel (221) and a second connecting channel (222) spanning the second processing area (122) are provided between the first processing area (121) and the aerobic culture area (220).
5. The method for acclimatizing and culturing salt-tolerant bacteria in saline wastewater according to claim 1, Features: The following steps are involved: During the flushing aeration process, the sewage in the aerobic zone (120) flows back into the anaerobic / anoxic zone (110) through the hydraulic fluctuations generated by the flushing aeration.
Citation Information
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