A bacteriostatic composition, bacteriostatic liquid, applications and methods of preparation

Abstract

The present invention relates to the field of skin surface antibacterial treatment, and more particularly to an antibacterial composition, its application, an antibacterial liquid prepared from the antibacterial composition, and a preparation method. The antibacterial composition comprises the following ingredients in proportions by weight: 0.1-1 part of a Forsythia suspensa extract, 0.1-1 part of a honeysuckle extract, 0.01-0.05 part of benzalkonium chloride, and 0.03-0.05 part of polyaminopropyl biguanide. The antibacterial composition exhibits significant antibacterial effects, is mild and safe, and exhibits minimal irritation, making it suitable for skin antibacterial treatment. The antibacterial composition can be used to prepare an antibacterial liquid for use on skin surfaces.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of bacteriostasis, in particular to a bacteriostatic composition, application thereof and a bacteriostatic liquid prepared from the bacteriostatic composition and a preparation method thereof. BACKGROUND

[0002] Skin is a good barrier against bacterial infection, but also an organ that is extremely susceptible to bacterial infection. A study once detected 51 people's hands in detail, and a total of 4742 kinds of bacteria were found, and there were more than 150 kinds of bacteria on average on each hand. Because the skin surface can contact a large number of bacteria, any skin damage can easily cause infection. The bacteria that infect the skin are mainly staphylococci and streptococci, and in hospitals, clinics, parks, exposed skin or swimming in pools, lakes and oceans, they can also be infected by other rare bacteria. Bacterial skin infection can only have local effects such as small pustules, or can spread and involve a large area of skin within a few hours, and even endanger life, such as staphylococcal skin scalded syndrome. There are some special groups of people whose skin infection risk is very high, for example: diabetic patients, whose blood circulation is poor, especially the skin of the hands and feet; AIDS patients, whose immune systems are suppressed. For such special groups of people, skin damage caused by sunburn, scratching or other irritation is easily infected by bacteria. Therefore, skin surface bacteriostasis is very important for preventing infection.

[0003] Products that can be used for skin surface bacteriostasis include 75% medical alcohol, 84 disinfectant, peracetic acid, lysol, potassium permanganate, etc. Among them, 75% medical alcohol will irritate the skin and absorb epidermal moisture, causing the skin to dry and form small wounds; potassium permanganate is purple, and pigments will adhere to the skin, and its concentrated solution or crystals are corrosive to the skin; 84 disinfectant contains chlorine and has strong corrosive effect, frequent contact with the hand will cause the palm to sweat a lot, the nails to become thin, the hair to fall out, and also have sensitization effect, and the free chlorine released may also cause poisoning; peracetic acid is unstable and has certain risk during storage, and it is also corrosive, which can cause skin damage when contacting the skin; lysol has a certain degree of irritation and corrosion, erosion, and toxicity to the skin. It can be seen that the products for skin surface bacteriostasis in the prior art have different degrees of irritation to the skin and can damage the skin. SUMMARY

[0004] In view of the problem that the current skin bacteriostatic products have irritation and damage to the skin, the present application provides a bacteriostatic composition, a bacteriostatic liquid, an application and a preparation method.

[0005] To achieve the above-mentioned application purposes, the embodiments of the present application adopt the following technical solutions:

[0006] In one aspect, the present application provides a bacteriostatic composition comprising the following ingredients in the following proportions by weight: 0.1-1 parts of forsythia suspensa extract, 0.1-1 parts of honeysuckle extract, 0.01-0.05 parts of benzalkonium chloride, and 0.03-0.05 parts of polyaminopropyl biguanide.

[0007] The benzalkonium chloride and the polyaminopropyl biguanide in the bacteriostatic composition have broad-spectrum and high-efficiency bactericidal capacity. The forsythia suspensa extract has broad-spectrum antibacterial effect and has strong inhibitory effect on Staphylococcus aureus and Shigella dysenteriae, and also has inhibitory effect on Salmonella typhi, Vibrio cholerae, Escherichia coli, Pseudomonas aeruginosa, Bordetella pertussis, Mycobacterium tuberculosis, Streptococcus pneumoniae, Streptococcus hemolyticus, and influenza virus, and has antifungal effect. The honeysuckle extract has certain inhibitory effect on various pathogenic bacteria such as Staphylococcus aureus, Streptococcus hemolyticus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae, Salmonella typhi, Salmonella paratyphi, etc., and has inhibitory and killing effect on Streptococcus pneumoniae, Streptococcus meningitidis, Pseudomonas aeruginosa, Mycobacterium tuberculosis, Shigella dysenteriae, Streptococcus mutans, etc., and can inhibit influenza virus, orphan virus, herpes virus, and Leptospira. The present application finds that, in the research, the forsythia suspensa extract and the honeysuckle extract are compounded with the benzalkonium chloride and the polyaminopropyl biguanide in the above-mentioned dosage range, and the obtained bacteriostatic composition not only has excellent bacteriostatic effect, but also can nourish and repair the skin, promote cell vitality, maintain the normal state of elastic fibers, collagen fibers, and protein polysaccharides, thereby reducing the irritation and damage of the benzalkonium chloride and the polyaminopropyl biguanide to the skin, making the obtained bacteriostatic composition mild and safe, and compared with the benzalkonium chloride and the polyaminopropyl biguanide used alone, the irritation of the bacteriostatic composition is significantly reduced, and the bacteriostatic composition can be used for bacteriostasis on the skin surface of hands, etc.

[0008] Preferably, the bacteriostatic composition comprises the following ingredients in the following proportions by weight: 0.1-0.3 parts of forsythia suspensa extract, 0.1-0.3 parts of honeysuckle extract, 0.02-0.03 parts of benzalkonium chloride, and 0.04-0.05 parts of polyaminopropyl biguanide.

[0009] Preferably, the bacteriostatic composition comprises the following ingredients in the following proportions by weight: 0.1 parts of forsythia suspensa extract, 0.1 parts of honeysuckle extract, 0.024 parts of benzalkonium chloride, and 0.04 parts of polyaminopropyl biguanide. The bacteriostatic composition with the above-mentioned proportions has more excellent bacteriostatic effect and no irritation.

[0010] Optionally, the preparation method of the forsythia suspensa extract is as follows: ultrasonic extraction of forsythia suspensa with 15-25% v / v ethanol aqueous solution at 25-35℃, first at a frequency of 25-35 kHz for 20-30 min, and then at a frequency of 50-70 kHz for 15-25 min; after the extraction, filtration is performed, and the obtained filtrate is concentrated to 1.2-1.3 g / ml to obtain the forsythia suspensa extract; wherein the mass of the 15-25% v / v ethanol aqueous solution is 5-8 times the mass of the forsythia suspensa.

[0011] The forsythia suspensa extract obtained by the above preparation method has good antibacterial effect, and can significantly reduce the irritation of the antibacterial composition when used together with the honeysuckle extract.

[0012] Optionally, the preparation method of the honeysuckle extract is as follows: ultrasonic extraction of honeysuckle with 45-65% v / v ethanol aqueous solution at 45-60℃, first at a frequency of 20-35 kHz for 20-30 min, and then at a frequency of 55-70 kHz for 15-20 min; after the extraction, filtration is performed, and the obtained filtrate is concentrated to 1.2-1.3 g / ml to obtain the honeysuckle extract; wherein the mass of the 45-65% v / v ethanol aqueous solution is 7-10 times the mass of the honeysuckle.

[0013] The honeysuckle extract obtained by the above preparation method has good antibacterial effect and can reduce the irritation of the antibacterial composition, and can further improve the antibacterial effect and reduce the irritation when used together with the forsythia suspensa extract obtained by the above preparation method.

[0014] In a second aspect, the embodiments of the present application also provide use of the above antibacterial composition in the preparation of skin antibacterial products.

[0015] The above antibacterial composition has excellent antibacterial effect, is mild, safe and non-irritating, and can be prepared into various skin antibacterial products, such as antibacterial spray, antibacterial gel, antibacterial wet wipes, etc.

[0016] In a third aspect, the embodiments of the present application also provide an antibacterial liquid comprising the above antibacterial composition. The antibacterial liquid can be directly applied to the surface of the skin, or can be filled into a spray device for use as a spray for the skin.

[0017] The antibacterial liquid comprises the following ingredients in parts by weight:

[0018]

[0019] The humectant is selected from at least one of propylene glycol, glycerol and butylene glycol.

[0020] The preservative is at least one of bis(hydroxymethyl) imidazolinyl urea, methylparaben, propylparaben. In the embodiment of the present application, the preservative preferably comprises bis(hydroxymethyl) imidazolinyl urea, methylparaben and propylparaben, preferably in the amount of 0.08-0.15 parts of bis(hydroxymethyl) imidazolinyl urea, 0.05-0.2 parts of methylparaben and 0.05-0.2 parts of propylparaben.

[0021] The bactericide is chlorphenesin.

[0022] The solubilizer is PEG-40 hydrogenated castor oil.

[0023] The antioxidant is ethylenediaminetetraacetic acid disodium salt (disodium EDTA).

[0024] The above preferred raw materials are all commonly used components of cosmetics in compliance with the regulations, and are safe to use in the above amount range without causing obvious discomfort to the skin.

[0025] In a fourth aspect, the present application further provides a preparation method of the above bacteriostatic solution, comprising the following steps:

[0026] The humectant is added to water and stirred until dissolved;

[0027] The bactericide, benzalkonium chloride and polyaminopropyl biguanide are added and stirred until uniformly dispersed;

[0028] The forsythia extract, honeysuckle extract, essence, solubilizer, antioxidant and preservative are added and mixed until uniform, to obtain the bacteriostatic solution.

[0029] The temperature for adding the humectant is preferably 75-85℃, which can accelerate the dissolution of the humectant and improve the production efficiency.

[0030] The solution temperature for adding the bactericide, benzalkonium chloride and polyaminopropyl biguanide is preferably 48-52℃, which can accelerate the dissolution and at the same time avoid the formation of impurities at high temperature.

[0031] The solution temperature for adding the forsythia extract, honeysuckle extract, essence and preservative is preferably 43-47℃, which can promote the mixing of the forsythia extract and honeysuckle extract with other components. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solutions and advantages of the present application more clear and explicit, the present application is further described in detail below in combination with specific examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0033] In the following examples, the experimental methods used are all conventional methods in the art unless otherwise specified.

[0034] The forsythia suspensa and honeysuckle used in the following examples all meet the relevant provisions of the Chinese Pharmacopoeia (2015 edition) Part I Text under the respective medicinal materials.

[0035] The raw materials, reagents and the like used in the following examples were obtained from commercial channels unless otherwise specified.

[0036] Example 1

[0037] The present example provides a bacteriostatic composition comprising the following ingredients in parts by weight:

[0038] 0.1 part of forsythia suspensa extract, 0.1 part of honeysuckle extract, 0.024 part of benzalkonium chloride, and 0.04 part of polyaminopropyl biguanide.

[0039] The preparation method of the forsythia suspensa extract is as follows: the forsythia suspensa is ultrasonically extracted with 20% v / v ethanol aqueous solution at 20°C, first at a frequency of 30 kHz for 25 min, and then at a frequency of 60 kHz for 20 min, and the mass of the 20% v / v ethanol aqueous solution is 7 times the mass of the forsythia suspensa; after the extraction is completed, the obtained filtrate is concentrated to 1.25 g / ml, and the forsythia suspensa extract is obtained.

[0040] The preparation method of the honeysuckle extract is as follows: the honeysuckle is ultrasonically extracted with 55% v / v ethanol aqueous solution at 50°C, first at a frequency of 25 kHz for 25 min, and then at a frequency of 60 kHz for 18 min, and the mass of the 55% v / v ethanol aqueous solution is 8 times the mass of the honeysuckle; after the extraction is completed, the obtained filtrate is concentrated to 1.25 g / ml, and the honeysuckle extract is obtained.

[0041] Example 2

[0042] The present example provides a bacteriostatic composition comprising the following ingredients in parts by weight:

[0043] 0.1 part of forsythia suspensa extract, 0.1 part of honeysuckle extract, 0.024 part of benzalkonium chloride, and 0.04 part of polyaminopropyl biguanide.

[0044] The preparation method of the forsythia suspensa extract is as follows: the forsythia suspensa is ultrasonically extracted with 15% v / v ethanol aqueous solution at 35°C, first at a frequency of 25 kHz for 30 min, and then at a frequency of 50 kHz for 25 min, and the mass of the 15% v / v ethanol aqueous solution is 5 times the mass of the forsythia suspensa; after the extraction is completed, the obtained filtrate is concentrated to 1.2 g / ml, and the forsythia suspensa extract is obtained.

[0045] The preparation method of the honeysuckle extract is as follows: the honeysuckle is ultrasonically extracted with 45% v / v ethanol aqueous solution at 60℃, first at a frequency of 20 kHz for 30 min, and then at a frequency of 55 kHz for 20 min, the mass of the 45% v / v ethanol aqueous solution is 7 times the mass of the honeysuckle; after the extraction is completed, the obtained filtrate is concentrated to 1.2 g / ml, and the honeysuckle extract is obtained.

[0046] Example 3

[0047] The embodiment of the present application provides a bacteriostatic composition, which comprises the following components in a proportion by weight fraction:

[0048] 0.1 parts of the forsythia suspensa extract, 0.1 parts of the honeysuckle extract, 0.024 parts of benzalkonium chloride and 0.04 parts of polyaminopropyl biguanide.

[0049] The preparation method of the forsythia suspensa extract is as follows: the forsythia suspensa is ultrasonically extracted with 25% v / v ethanol aqueous solution at 25℃, first at a frequency of 35 kHz for 20 min, and then at a frequency of 70 kHz for 15 min, the mass of the 25% v / v ethanol aqueous solution is 8 times the mass of the forsythia suspensa; after the extraction is completed, the obtained filtrate is concentrated to 1.3 g / ml, and the forsythia suspensa extract is obtained.

[0050] The preparation method of the honeysuckle extract is as follows: the honeysuckle is ultrasonically extracted with 65% v / v ethanol aqueous solution at 45℃, first at a frequency of 35 kHz for 20 min, and then at a frequency of 70 kHz for 15 min, the mass of the 65% v / v ethanol aqueous solution is 10 times the mass of the honeysuckle; after the extraction is completed, the obtained filtrate is concentrated to 1.3 g / ml, and the honeysuckle extract is obtained.

[0051] Example 4

[0052] The embodiment of the present application provides a bacteriostatic composition, which comprises the following components in a proportion by weight fraction:

[0053] 0.1 parts of the forsythia suspensa extract, 0.1 parts of the honeysuckle extract, 0.02 parts of benzalkonium chloride and 0.04 parts of polyaminopropyl biguanide.

[0054] The preparation method of the forsythia suspensa extract and the honeysuckle extract is the same as that in the embodiment 1.

[0055] Example 5

[0056] The embodiment of the present application provides a bacteriostatic composition, which comprises the following components in a proportion by weight fraction:

[0057] 0.3 parts of the forsythia suspensa extract, 0.3 parts of the honeysuckle extract, 0.02 parts of benzalkonium chloride and 0.04 parts of polyaminopropyl biguanide.

[0058] The preparation method of the forsythia extract and the honeysuckle extract is the same as that in Embodiment 1.

[0059] Embodiment 6

[0060] The bacteriostatic composition provided by the embodiment of the application comprises the following components in the following proportions by weight:

[0061] The forsythia extract is 0.2 parts, the honeysuckle extract is 0.2 parts, the benzalkonium chloride is 0.03 parts, and the polyaminopropyl biguanide is 0.05 parts.

[0062] The preparation method of the forsythia extract and the honeysuckle extract is the same as that in Embodiment 1.

[0063] Embodiment 7

[0064] The bacteriostatic composition provided by the embodiment of the application comprises the following components in the following proportions by weight:

[0065] The forsythia extract is 0.1 parts, the honeysuckle extract is 0.1 parts, the benzalkonium chloride is 0.01 parts, and the polyaminopropyl biguanide is 0.03 parts.

[0066] The preparation method of the forsythia extract and the honeysuckle extract is the same as that in Embodiment 1.

[0067] Embodiment 8

[0068] The bacteriostatic composition provided by the embodiment of the application comprises the following components in the following proportions by weight:

[0069] The forsythia extract is 1 part, the honeysuckle extract is 1 part, the benzalkonium chloride is 0.05 parts, and the polyaminopropyl biguanide is 0.05 parts.

[0070] The preparation method of the forsythia extract and the honeysuckle extract is the same as that in Embodiment 1.

[0071] Embodiment 9

[0072] The bacteriostatic liquid for the skin surface and the preparation method thereof provided by the embodiment of the application comprise the following components in the following proportions by weight:

[0073]

[0074] The preparation method of the forsythia extract and the honeysuckle extract is the same as that in Embodiment 1.

[0075] The preparation method of the bacteriostatic liquid is as follows:

[0076] Add propylene glycol to water, and stir to completely dissolve at 80 ℃;

[0077] Cool to 50 ℃, and add chlorphenesin, benzalkonium chloride, and polyaminopropyl biguanide, and stir to uniformly disperse;

[0078] Cool to 45℃, add forsythia suspense extract and honeysuckle extract, and essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid methyl ester and hydroxybenzyl propyl ester;

[0079] Cool to 40℃, and the bacteriostatic solution is obtained.

[0080] Example 10

[0081] The embodiment of the present application provides a bacteriostatic solution for skin surface and a preparation method thereof, the bacteriostatic solution comprises the following components in a proportion by weight:

[0082]

[0083]

[0084] The preparation method of forsythia suspense extract and honeysuckle extract is the same as that in Example 2.

[0085] The preparation method of the bacteriostatic solution is as follows:

[0086] Add propylene glycol and glycerol into water, and stir to completely dissolve at 75℃;

[0087] Cool to 48℃, and add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, and stir to uniformly disperse;

[0088] Cool to 43℃, and add forsythia suspense extract and honeysuckle extract, and essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid methyl ester and hydroxybenzyl propyl ester;

[0089] Cool to 40℃, and the bacteriostatic solution is obtained.

[0090] Example 11

[0091] The embodiment of the present application provides a bacteriostatic solution for skin surface and a preparation method thereof, the bacteriostatic solution comprises the following components in a proportion by weight:

[0092]

[0093]

[0094] The preparation method of forsythia suspense extract and honeysuckle extract is the same as that in Example 3.

[0095] The preparation method of the bacteriostatic solution is as follows:

[0096] Add propylene glycol and glycerol into water, and stir to completely dissolve at 75℃;

[0097] Cool to 52℃, and add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, and stir to uniformly disperse;

[0098] Cool to 46℃, add the extract of forsythia and honeysuckle, and essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0099] Cool to 40℃, and the bacteriostatic solution is obtained.

[0100] Example 12

[0101] The bacteriostatic solution for skin surface and the preparation method thereof provided by the embodiment of the application include the following ingredients in the following weight ratio:

[0102]

[0103]

[0104] The preparation method of the extract of forsythia and honeysuckle is the same as that in Example 4.

[0105] The preparation method of the bacteriostatic solution is as follows:

[0106] Add butylene glycol to water, and stir to completely dissolve at 85℃;

[0107] Cool to 48℃, and add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, and stir to uniformly disperse;

[0108] Cool to 44℃, and add the extract of forsythia and honeysuckle, and essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0109] Cool to 40℃, and the bacteriostatic solution is obtained.

[0110] Example 13

[0111] The bacteriostatic solution for skin surface and the preparation method thereof provided by the embodiment of the application include the following ingredients in the following weight ratio:

[0112]

[0113]

[0114] The preparation method of the extract of forsythia and honeysuckle is the same as that in Example 5.

[0115] The preparation method of the bacteriostatic solution is as follows:

[0116] Add butylene glycol to water, and stir to completely dissolve at 85℃;

[0117] Cool down to 51℃, add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, stir and disperse evenly;

[0118] Cool down to 47℃, add extract of forsythia suspensa and honeysuckle, essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0119] Cool down to 40℃, and the bacteriostatic solution is obtained.

[0120] Example 14

[0121] The bacteriostatic solution for skin surface and the preparation method thereof provided by the embodiments of the present application include the following ingredients in the following weight ratio:

[0122]

[0123]

[0124] The preparation method of the extract of forsythia suspensa and honeysuckle is the same as that in Example 6.

[0125] The preparation method of the bacteriostatic solution is as follows:

[0126] Add glycerol to water, and heat to 82℃ to completely dissolve under stirring;

[0127] Cool down to 51℃, add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, stir and disperse evenly;

[0128] Cool down to 43℃, add extract of forsythia suspensa and honeysuckle, essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0129] Cool down to 40℃, and the bacteriostatic solution is obtained.

[0130] Example 15

[0131] The bacteriostatic solution for skin surface and the preparation method thereof provided by the embodiments of the present application include the following ingredients in the following weight ratio:

[0132]

[0133] The preparation method of the extract of forsythia suspensa and honeysuckle is the same as that in Example 7.

[0134] The preparation method of the bacteriostatic solution is as follows:

[0135] Add glycerol to water, and heat to 83℃ to completely dissolve under stirring;

[0136] Cool down to 49℃, add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, stir and disperse evenly;

[0137] Cool down to 44℃, add extract of forsythia suspensa and honeysuckle, essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0138] Cool down to 40℃, and the bacteriostatic solution is obtained.

[0139] Example 16

[0140] The bacteriostatic solution for skin surface and its preparation method are provided by the embodiments of the present application, and the bacteriostatic solution comprises the following components in the following weight ratio:

[0141]

[0142] The preparation method of the extract of forsythia suspensa and the extract of honeysuckle is the same as that in Example 8.

[0143] The preparation method of the bacteriostatic solution is as follows:

[0144] Add butylene glycol to water, and stir to completely dissolve at 78℃;

[0145] Cool down to 49℃, add chlorphenesin, benzalkonium chloride, polyaminopropyl biguanide, stir and disperse evenly;

[0146] Cool down to 46℃, add extract of forsythia suspensa and honeysuckle, essence, PEG-40 hydrogenated castor oil, disodium EDTA, bis (hydroxymethyl) imidazole alkyl urea, hydroxybenzoic acid ester and hydroxybenzyl propyl ester;

[0147] Cool down to 40℃, and the bacteriostatic solution is obtained.

[0148] Comparative Example 1

[0149] The bacteriostatic composition provided by the present comparative example comprises the same components as those in Example 1.

[0150] The preparation method of the extract of forsythia suspensa is as follows: forsythia suspensa is decocted with 10 times of water for 2 times, 30 min each time; the filtrates are combined and concentrated to 1.2-1.3 g / ml, and the extract of forsythia suspensa is obtained.

[0151] The preparation method of the extract of honeysuckle is the same as that in Example 1.

[0152] Comparative Example 2

[0153] The bacteriostatic composition provided by the present comparative example comprises the same components as those in Example 1.

[0154] The preparation method of the forsythia extract is as follows: forsythia is extracted with 7 times of 55% v / v ethanol aqueous solution by heating and refluxing for 2 times, 30 min each time; the filtrate is filtered and combined, and concentrated to 1.25 g / ml, to obtain the forsythia extract.

[0155] The preparation method of the honeysuckle extract is the same as that in Example 1.

[0156] Comparative Example 3

[0157] This comparative example provides a bacteriostatic composition, which has the same components as those in Example 1.

[0158] The preparation method of the forsythia extract is as follows: forsythia is extracted with 7 times of 20% v / v ethanol aqueous solution by ultrasonic extraction for 45 min, and the ultrasonic frequency is 45 Hz; the filtrate is filtered and concentrated to 1.25 g / ml, to obtain the forsythia extract.

[0159] The preparation method of the honeysuckle extract is the same as that in Example 1.

[0160] Comparative Example 4

[0161] This comparative example provides a bacteriostatic composition, which has the same components as those in Example 1.

[0162] The preparation method of the forsythia extract is the same as that in Example 1.

[0163] The preparation method of the honeysuckle extract is as follows: honeysuckle is extracted with 10 times of water by decocting for 2 times, 30 min each time; the filtrate is filtered and combined, and concentrated to 1.2-1.3 g / ml, to obtain the honeysuckle extract.

[0164] Comparative Example 5

[0165] This comparative example provides a bacteriostatic composition, which has the same components as those in Example 1.

[0166] The preparation method of the forsythia extract is the same as that in Example 1.

[0167] The preparation method of the honeysuckle extract is as follows: honeysuckle is extracted with 7 times of 57% v / v ethanol aqueous solution by heating and refluxing for 2 times, 30 min each time; the filtrate is filtered and combined, and concentrated to 1.25 g / ml, to obtain the honeysuckle extract.

[0168] Comparative Example 6

[0169] This comparative example provides a bacteriostatic composition, which has the same components as those in Example 1.

[0170] The preparation method of the forsythia extract is the same as that in Example 1.

[0171] The preparation method of the honeysuckle extract is as follows: honeysuckle is extracted with 8 times of 55% v / v ethanol aqueous solution by ultrasonic extraction for 45 min, and the ultrasonic frequency is 45 Hz; the filtrate is filtered and concentrated to 1.25 g / ml, to obtain the honeysuckle extract.

[0172] Comparative Example 7

[0173] This comparative example provides a bacteriostatic composition comprising the following ingredients in parts by weight:

[0174] 0.1 part of Huanglian extract, 0.1 part of Honeysuckle extract, 0.024 part of benzalkonium chloride and 0.04 part of polyaminopropyl biguanide.

[0175] The preparation method of the Honeysuckle extract is the same as that of Example 1.

[0176] The preparation method of the Huanglian extract is as follows: Huanglian is ultrasonically extracted with 20% v / v aqueous ethanol solution at 20°C, first at a frequency of 30 kHz for 25 min and then at a frequency of 60 kHz for 20 min; the mass of the 20% v / v aqueous ethanol solution is 7 times the mass of the Huanglian; after the extraction is completed, the resulting filtrate is concentrated to 1.25 g / ml, and the Huanglian extract is obtained.

[0177] Comparative Example 8

[0178] This comparative example provides a bacteriostatic composition comprising the following ingredients in parts by weight:

[0179] 0.1 part of Huanglian extract, 0.1 part of Honeysuckle extract, 0.024 part of benzalkonium chloride and 0.04 part of polyaminopropyl biguanide.

[0180] The preparation method of the Honeysuckle extract is the same as that of Example 1.

[0181] Comparative Example 9

[0182] This comparative example provides a bacteriostatic solution for the surface of the skin and a preparation method thereof, the ingredient ratio and preparation method of the bacteriostatic solution being the same as those of Example 9, and the preparation methods of the Honeysuckle extract and the Huanglian extract being the same as those of Comparative Example 1.

[0183] Comparative Example 10

[0184] This comparative example provides a bacteriostatic solution for the surface of the skin and a preparation method thereof, the ingredient ratio and preparation method of the bacteriostatic solution being the same as those of Example 9, and the preparation methods of the Honeysuckle extract and the Huanglian extract being the same as those of Comparative Example 2.

[0185] Comparative Example 11

[0186] This comparative example provides a bacteriostatic solution for the surface of the skin and a preparation method thereof, the ingredient ratio and preparation method of the bacteriostatic solution being the same as those of Example 9, and the preparation methods of the Honeysuckle extract and the Huanglian extract being the same as those of Comparative Example 3.

[0187] Comparative Example 12

[0188] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 4.

[0189] Comparative Example 13

[0190] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 5.

[0191] Comparative Example 14

[0192] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 6.

[0193] Comparative Example 15

[0194] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 7.

[0195] Comparative Example 16

[0196] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 8.

[0197] Comparative Example 17

[0198] The present comparative example provides a bacteriostatic solution for skin surface and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspense extract and the honeysuckle extract are the same as those of Comparative Example 8.

[0199] Test Example 1

[0200] The present test example investigates the bacteriostatic effect of the bacteriostatic compositions prepared in Examples 1-8 and Comparative Examples 1-8 above.

[0201] The component ratio of the nutrient agar medium used in the following experiment is as follows: 10 g of protein peptone, 3 g of beef extract, 5 g of sodium chloride, 17 g of agar, and 1000 mL of distilled water;

[0202] The composition of the Sabouraud medium is: 10 g of proteose peptone, 20 g of agar, 40 g of glucose, 1 L of distilled water.

[0203] Test strains: Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), Candida albicans (ATCC 10231).

[0204] 1. Experimental method

[0205] The 5th generation nutrient agar medium slant culture (24 h) of the test bacteria was washed with 5 mL of 0.03 mol / L phosphate buffer (hereinafter referred to as PBS) to remove the bacterial mat, diluted with the above PBS, and prepared into a bacterial suspension with a concentration of 5 x 104 cfu / ml.

[0206] The bacteriostatic composition samples prepared from Examples 1 to 8 and Comparative Examples 1 to 8 were used as the test sample liquid, and 4 tubes of the test sample liquid (5 mL) and the control sample liquid (having the same composition as the test sample liquid, but not containing forsythia extract, honeysuckle extract, benzalkonium chloride and polyaminopropyl biguanide, and sterilized) were taken.

[0207] 100 μL of the above bacterial suspension was added dropwise into each of the test sample liquid and the control sample liquid, mixed uniformly, and the timing was started. After 2 min, 5 min, 10 min and 20 min of action, 0.5 mL of the mixed sample was taken with sterile forceps and placed in a test tube containing 5 mL of PBS, mixed thoroughly, and 0.5 mL was taken and placed on two plates. Nutrient agar medium (bacteria) or Sabouraud agar medium (yeast) at a cool temperature of 40-50°C, 15 mL, was used for pouring, the plates were rotated to ensure uniformity, and after the agar was solidified, the plates were inverted and incubated at 35°C ± 2°C for 48 h (bacteria) or 72 h (yeast). The viable bacterial colonies were counted.

[0208] The test was repeated 3 times, and the bacteriostatic rate was calculated according to the following formula:

[0209] X = (A-B) / A x 100%;

[0210] In the formula, X is the bacteriostatic rate, A is the average number of colonies of the control sample liquid, and B is the average number of colonies of the test sample liquid.

[0211] 2. Experimental results

[0212] The average bacteriostatic rate of each bacteriostatic composition at different action times was recorded, and the results are shown in Tables 1 to 3.

[0213] Table 1 Bacteriostatic data against Staphylococcus aureus

[0214]

[0215] Table 2 Bacteriostatic data against Escherichia coli

[0216]

[0217]

[0218] Table 3: Bacteriostatic data of Candida albicans

[0219]

[0220] From the results of Tables 1-3, it can be seen that the bacteriostatic compositions obtained in Examples 1-8 have a bacteriostatic rate of >99.9% for S. aureus, E. coli and C. albicans at 20 min, and are superior to the bacteriostatic compositions obtained in the respective comparative examples.

[0221] Test Example 2

[0222] This test example investigates the irritation of the bacteriostatic liquid for the skin surface prepared from Examples 9-16 and Comparative Examples 9-17.

[0223] 1. Experimental method

[0224] Fifty-one New Zealand rabbits with intact skin, female, weighing 2.0-2.5 kg, were fed with ordinary grade rabbit maintenance feed at a temperature of 20-26°C and a relative humidity of 40%-70%.

[0225] According to the "Disinfection Technical Specification" (2002 edition) 2.3.3.3.1, the bacteriostatic liquids prepared from Examples 9-16 and Comparative Examples 9-17 were subjected to a complete skin irritation test (3 rabbits were used for each sample): about 24 hours before the experiment, the fur on both sides of the rabbit's back was removed without damaging the skin, and the fur was removed in an area of about 3 cm x 3 cm on both the left and right sides. 0.5 ml of the test liquid was applied to the skin, then covered with two layers of gauze and one layer of glass paper, and fixed with adhesive tape. The other side of the skin was applied with 0.5 ml of distilled water as a blank control. The application time was 4 hours, and the test material was removed by washing with warm water for 1 min. The local skin reactions were observed at 1 hour, 24 hours and 48 hours after the test material was removed, and the skin irritation reaction score was evaluated according to Table 4. The results are shown in Tables 5 and 6.

[0226] Table 4: Skin irritation reaction score criteria

[0227]

[0228] Table 5: Test results of the bacteriostatic liquid obtained in Examples 9-16

[0229]

[0230] Table 6: Test results of the bacteriostatic liquid obtained in Comparative Examples 9-17

[0231]

[0232]

[0233] As can be seen from the results of Table 5 and Table 6, the bacteriostatic solution obtained in Examples 9-16 has no irritation to the skin of white rabbits, and the bacteriostatic solution obtained in Comparative Examples 9-17 has irritation to the skin of white rabbits.

[0234] As can be seen from the experimental results of Test Example 1 and Test Example 2, the bacteriostatic composition provided by the examples has more effective bacteriostatic effect, and the bacteriostatic solution for the skin surface prepared from the bacteriostatic composition has no irritation to the skin.

[0235] The above only describes the preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement or improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An antibacterial composition, characterized in that The invention is composed of the following ingredients in parts by weight: 0.1-0.3 parts of forsythia extract, 0.1-0.3 parts of honeysuckle extract, 0.01-0.05 parts of benzalkonium chloride and 0.03-0.05 parts of polyaminopropyl biguanide; The preparation method of the Forsythia suspensa extract comprises: subjecting the Forsythia suspensa to ultrasonic extraction at 25-35° C. using a 15-25% v / v ethanol aqueous solution: first extracting at a frequency of 25-35 kHz for 20-30 minutes, then extracting at a frequency of 50-70 kHz for 15-25 minutes; filtering after the extraction, and concentrating the resulting filtrate to 1.2-1.3 g / ml to obtain the Forsythia suspensa extract; wherein the mass of the 15-25% v / v ethanol aqueous solution is 5-8 times the mass of the Forsythia suspensa; The preparation method of the honeysuckle extract is as follows: ultrasonically extracting honeysuckle with a 45-65% v / v ethanol aqueous solution at 45-60° C.: first extracting at a frequency of 20-35 kHz for 20-30 minutes, and then extracting at a frequency of 55-70 kHz for 15-20 minutes; after the extraction is completed, filtering and concentrating the obtained filtrate to 1.2-1.3 g / ml to obtain the honeysuckle extract; wherein the mass of the 45-65% v / v ethanol aqueous solution is 7-10 times the mass of the honeysuckle.

2. The antibacterial composition according to claim 1, characterized in that The invention is composed of the following components in parts by weight: 0.1-0.3 parts of forsythia extract, 0.1-0.3 parts of honeysuckle extract, 0.02-0.03 parts of benzalkonium chloride and 0.04-0.05 parts of polyaminopropyl biguanide.

3. The antibacterial composition according to claim 2, characterized in that The invention is composed of the following components in parts by weight: 0.1 part of forsythia extract, 0.1 part of honeysuckle extract, 0.024 part of benzalkonium chloride and 0.04 part of polyaminopropyl biguanide.

4. Use of the antibacterial composition according to any one of claims 1 to 3 in the preparation of an antibacterial product for skin use.

5. An antibacterial solution, characterized in that The invention comprises the antibacterial composition according to claim 1.

6. The antibacterial liquid according to claim 5, characterized in that The composition comprises the following components in parts by weight: 80-95 parts water; 3~6 parts of moisturizer; 0.18~0.55 parts of preservatives; Chlorphenesin 0.03~0.08 parts; Solubilizer 0.02~0.3 parts; 0.1~0.3 parts of antioxidant; 0.05~0.3 parts of flavor; 0.01-0.05 parts of benzalkonium chloride; Polyaminopropyl biguanide 0.03-0.05 parts; 0.1-0.3 parts of Forsythia suspensa extract; 0.1~0.3 parts of honeysuckle extract.

7. The antibacterial solution according to claim 6, characterized in that The moisturizing agent is selected from at least one of propylene glycol, glycerin and butylene glycol; and / or The preservative is selected from at least one of bis(hydroxymethyl)imidazolidinyl urea, methylparaben, and propylparaben; and / or The solubilizing agent is PEG-40 hydrogenated castor oil; and / or The antioxidant is disodium ethylenediaminetetraacetic acid.

8. The method for preparing the antibacterial solution according to claim 6 or 7, characterized in that: The following steps are involved: Add the moisturizing agent to the water and stir until dissolved; Add the chlorphenesin, benzalkonium chloride and polyaminopropyl biguanide, and stir to disperse them evenly; Add the forsythia extract, honeysuckle extract, essence, solubilizer, antioxidant and preservative, mix evenly, and obtain the antibacterial solution.

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