A skin care bacteriostatic composition, a skin care bacteriostatic liquid, applications and preparation methods

Abstract

The present application relates to the technical field of skin bacteriostasis, and particularly relates to a skin care bacteriostasis composition, application of the skin care bacteriostasis composition, and a skin care bacteriostasis liquid prepared from the skin care bacteriostasis composition and a preparation method of the skin care bacteriostasis liquid. The skin care bacteriostasis composition comprises the following components in parts by weight: 0.1-1 parts of grain extract, 0.1-0.5 parts of forsythia suspensa extract, 0.1-0.5 parts of honeysuckle extract, 0.05-0.5 parts of phenoxyethanol, and 0.01-0.05 parts of benzalkonium chloride. The skin care bacteriostasis composition has no irritation to the skin, can gently clean the skin, kill bacteria and inhibit bacteria, and has the skin care effects of moisturizing and nourishing, and can be used to prepare a cleaning and bacteriostasis product for hand or body skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the skin bacteriostatic technical field, and in particular to a skin care bacteriostatic composition, application thereof and a skin care bacteriostatic liquid prepared from the skin care bacteriostatic composition and a preparation method of the skin care bacteriostatic liquid. BACKGROUND

[0002] Hand sanitizer is a daily hand washing product, which overcomes the problems of easy sliding of soap during use, difficulty in storage after use, high natural consumption, easy contamination of bacteria left by others after use and secondary pollution. Ordinary hand sanitizer only has cleaning function and does not have bacteriostatic effect. Most of the bacteriostatic hand sanitizers on the market currently add a large amount of chemical antibacterial agents, which often cause irritation to the skin. When the skin has small wounds (such as cracks, epidermal abrasions, etc.) or inflammation (such as solar dermatitis caused by sun exposure, etc.), a certain stinging sensation is caused. On the other hand, the cleaning power of hand sanitizer with mild oil-removing ingredients is difficult to meet expectations, so the cleaning ingredients in the hand sanitizer mostly use surfactants such as sodium dodecyl sulfate with strong oil-removing ability, which often damages the skin due to the over-strong oil-removing ability. However, the hand sanitizer with skin care ingredients often has skin care ingredients that are not easy to rinse off and are often left behind, which not only produces a greasy feeling, but also makes the skin dirt stay on the skin with the skin care ingredients, reducing the cleaning effect. SUMMARY

[0003] In view of the problems that the current bacteriostatic hand sanitizer has skin irritation and may damage the skin, the skin care ingredients are easy to remain and reduce the cleaning effect, the present application provides a skin care bacteriostatic composition, a skin care bacteriostatic liquid, an application and a preparation method.

[0004] To achieve the above-mentioned application purposes, the embodiments of the present application adopt the following technical solutions:

[0005] On the one hand, the present application provides a skin care bacteriostatic composition, which comprises the following ingredients in parts by weight: 0.1-1 parts of grain extract, 0.1-0.5 parts of forsythia suspensa extract, 0.1-0.5 parts of honeysuckle extract, 0.05-0.5 parts of phenoxyethanol and 0.01-0.05 parts of benzalkonium chloride.

[0006] In the skin care bacteriostatic composition, benzalkonium chloride is a cationic quaternary ammonium salt surfactant, which has a broad-spectrum killing effect on pathogenic bacteria, fungi, viruses, such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Candida albicans, Aspergillus niger, and SARS virus, MERSr-CoV, influenza virus (type A, type B), parainfluenza virus (PSV-1, 2, 3), avian influenza virus (H7N9), Newcastle disease virus, bursa disease virus, adenovirus, HIV virus, HSV-2, enterovirus, cytomegalovirus, BK virus, poliovirus (PV), coxsackie virus, Ebola virus, etc., and has little irritation to skin, mucosa and wounds, but is ineffective against Pseudomonas aeruginosa. Phenoxyethanol has bacteriostatic effect on both gram-positive and gram-negative bacteria, and has specific bacteriostatic effect on Pseudomonas aeruginosa, but relatively poor inhibitory effect on fungi.

[0007] The forsythia extract has different degrees of inhibitory effect on Staphylococcus aureus, Shigella dysenteriae, Salmonella typhi, Vibrio cholerae, Escherichia coli, Pseudomonas aeruginosa, Bordetella pertussis, Mycobacterium tuberculosis, Streptococcus pneumoniae, hemolytic streptococcus, and influenza virus, and also has antifungal effect. The honeysuckle extract has certain inhibitory and killing effect on various pathogenic bacteria such as Staphylococcus aureus, hemolytic streptococcus, Escherichia coli, dysentery bacillus, Vibrio cholerae, Salmonella typhi, paratyphoid bacillus, pneumococcus, meningococcus, Pseudomonas aeruginosa, Mycobacterium tuberculosis, Shigella dysenteriae, Streptococcus mutans, and can inhibit influenza virus, orphan virus, herpes virus, leptospira.

[0008] The present application has a synergistic effect after the combination of benzalkonium chloride, phenoxyethanol, forsythia extract and honeysuckle extract, and can achieve high bacteriostatic effect at a low dosage.

[0009] The grain extract is rich in nutritional ingredients in grains and has the effects of nourishing and repairing skin. The forsythia extract and the honeysuckle extract can enhance skin elasticity and promote mucosa repair. It is found in the research that the combination of the forsythia extract, the honeysuckle extract and the grain extract can make the composition non-irritating to skin, and will not cause stinging sensation even when there are small wounds or inflammation on the skin.

[0010] As a cationic quaternary ammonium salt surfactant, benzalkonium chloride has the effects of reducing surface tension and improving wettability, can improve the easy rinsing property of the composition, and reduce the residue of the composition on the skin surface.

[0011] Therefore, the composition prepared by the above-mentioned proportioning is non-irritating to skin, can clean skin gently, kill and inhibit bacteria, and has the effects of moisturizing and nourishing skin, and can be used for preparing a hand or body skin cleaning and bacteriostatic product.

[0012] Preferably, the skin care bacteriostatic composition comprises the following ingredients in the following proportions by weight: 0.2-0.8 parts of grain extract, 0.1-0.4 parts of forsythia suspensa extract, 0.1-0.4 parts of honeysuckle extract, 0.05-0.3 parts of phenoxyethanol, and 0.01-0.3 parts of benzalkonium chloride.

[0013] Preferably, the skin care bacteriostatic composition comprises the following ingredients in the following proportions by weight: 0.8 parts of grain extract, 0.4 parts of forsythia suspensa extract, 0.4 parts of honeysuckle extract, 0.3 parts of phenoxyethanol, and 0.1 parts of benzalkonium chloride. The skin care bacteriostatic composition obtained from the above ingredients has a higher bacteriostatic effect and better moisturizing and nourishing effect on the skin.

[0014] Optionally, the method for preparing the grain extract comprises the following steps:

[0015] S1, crushing corn and oats, soaking in water for 18-26 hours, draining the water, steaming the soaked corn and oats for 45 minutes to 1 hour, cooling to 37-42°C, adding lactobacillus casei, lactobacillus bulgaricus and saccharomyces cerevisiae, mixing uniformly, and then fermenting at 37-42°C for 20-26 hours, filtering, heating the obtained filtrate to reflux and maintaining reflux for 0.5-1 hour to obtain a fermentation liquor; the mass ratio of the corn to the oats is 1-2:1; the mass of the lactobacillus casei is 0.5-1% of the total mass of the corn and oats, the mass of the lactobacillus bulgaricus is 0.8-1.5% of the total mass of the corn and oats, and the mass of the saccharomyces cerevisiae is 1-1.5% of the total mass of the corn and oats;

[0016] S2, crushing soybeans, adding water equivalent to 6-9 times the mass of the soybeans, microwave extracting at a power of 750-850 W and a temperature of 35-45°C for 10-15 minutes, adding lipase and neutral protease, enzymatically hydrolyzing at 45-55°C for 2-3 hours, heating to 90-95°C for 5-10 minutes to inactivate the enzymes, and filtering to obtain an enzymatic hydrolysate; the mass of the lipase is 1.2-1.5% of the mass of the soybeans, and the mass of the neutral protease is 1.0-1.3% of the mass of the soybeans;

[0017] S3, concentrating the fermentation liquor obtained in step S1 and the enzymatic hydrolysate obtained in step S2 to 1.1-1.2 g / ml respectively to obtain concentrated fermentation liquor and concentrated enzymatic hydrolysate, and mixing the concentrated fermentation liquor and the concentrated enzymatic hydrolysate in a ratio of 1:1-1.5 to obtain the grain extract.

[0018] The grain extract liquid prepared by the preparation method is rich in fermentation products of corn and oat and enzymatic products of soybean, can effectively activate skin cells, accelerate skin cell repair, help to improve the dry and rough state of the skin, and can stimulate the skin to produce ceramide, thereby improving the skin's ability to absorb and retain water, and the composition has more excellent skin care effect.

[0019] Optionally, the preparation method of the forsythia suspensa extract liquid is: ultrasonic extraction of forsythia suspensa with 15-25% v / v ethanol aqueous solution at 25-35 DEG C, first at a frequency of 25-35 kHz for 20-30 min, and then at a frequency of 50-70 kHz for 15-25 min; after extraction, filtration is performed, and the obtained filtrate is concentrated to 1.2-1.3 g / ml to obtain the forsythia suspensa extract liquid; wherein the mass of the 15-25% v / v ethanol aqueous solution is 5-8 times the mass of the forsythia suspensa.

[0020] Optionally, the preparation method of the honeysuckle extract liquid is: ultrasonic extraction of honeysuckle with 45-65% v / v ethanol aqueous solution at 45-60 DEG C, first at a frequency of 20-35 kHz for 20-30 min, and then at a frequency of 55-70 kHz for 15-20 min; after extraction, filtration is performed, and the obtained filtrate is concentrated to 1.2-1.3 g / ml to obtain the honeysuckle extract liquid; wherein the mass of the 45-65% v / v ethanol aqueous solution is 7-10 times the mass of the honeysuckle.

[0021] The forsythia suspensa extract liquid and the honeysuckle extract liquid obtained by the above preparation method both have better bacteriostatic effect, and have the effects of reducing the irritation of the skin care bacteriostatic composition and improving the moisturizing and nourishing effect of the skin care bacteriostatic composition.

[0022] In a second aspect, the embodiments of the present application also provide the use of the above-mentioned skin care bacteriostatic composition in the preparation of a skin care bacteriostatic product.

[0023] The above-mentioned skin care bacteriostatic composition has excellent bacteriostatic effect, and has the effects of mild cleaning, moisturizing and nourishing the skin, and can be prepared into a skin care bacteriostatic product for daily use, such as a hand sanitizer, a shower gel, etc.

[0024] In a third aspect, the embodiments of the present application also provide a skin care bacteriostatic liquid, which comprises the above-mentioned skin care bacteriostatic composition. The skin care bacteriostatic liquid can be filled into a foaming bottle for use as a foaming hand sanitizer.

[0025] The skin care bacteriostatic liquid comprises the following ingredients in parts by weight:

[0026]

[0027]

[0028] The skin care bacteriostatic liquid uses alkyl glycoside surfactant as the main cleaning component, which is mild, non-toxic, non-irritating and easily biodegradable. The skin care bacteriostatic liquid also contains synergistic components such as grain extract, forsythia extract, honeysuckle extract, phenoxyethanol and benzalkonium chloride. Even with a small amount of bacteriostatic components, the bacteriostatic rate of escherichia coli, staphylococcus aureus and candida albicans can reach 99.9%. Glycerin is used as a moisturizing component to further prevent skin dryness after cleaning. The skin care bacteriostatic liquid prepared from the above components is easy to rinse and not easy to leave residues, and does not produce a slippery feeling when rinsing.

[0029] Preferably, the skin care bacteriostatic liquid comprises the following ingredients in the following proportions by weight:

[0030]

[0031] Further preferably, the skin care bacteriostatic liquid comprises the following ingredients in the following proportions by weight:

[0032]

[0033] In a fourth aspect, the present application also provides a preparation method of the above-mentioned skin care bacteriostatic liquid, comprising the following steps:

[0034] The grain extract, forsythia extract, honeysuckle extract, phenoxyethanol, benzalkonium chloride and 25-35% of the formula amount of deionized water are pre-mixed to obtain a pre-mixture;

[0035] Under stirring, the remaining formula amount of deionized water is added with the following ingredients: cocamidopropyl betaine, lauryl glucoside, decyl glucoside, glycerin and PEG-7 glyceryl cocoate. After stirring until complete dissolution and uniform mixing, vacuum degassing is performed;

[0036] The pre-mixture is added to the obtained material, and stirring is performed until uniform, followed by filtration to obtain the skin care bacteriostatic liquid.

[0037] Preferably, the remaining formula amount of deionized water is added with cocamidopropyl betaine, lauryl glucoside, decyl glucoside, glycerin and PEG-7 glyceryl cocoate at a stirring speed of 45-55 r / min. This ensures mixing efficiency while avoiding excessive bubbles and increasing degassing time. Then, the temperature is increased to 50-60°C to accelerate dissolution, and stirring is performed for another 10-25 min to achieve complete dissolution and uniform mixing. When the temperature of the material is lowered to 40-45°C, the pre-mixture is added. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solutions and advantages of the present application clearer and more apparent, the present application will be further described in detail below in combination with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0039] The experimental methods used in the following examples are conventional methods in the art, unless otherwise specified.

[0040] The forsythia suspensa and honeysuckle used in the following examples all meet the relevant provisions of the Chinese Pharmacopoeia (2015 edition) Part I Text under each medicinal material.

[0041] The raw materials, reagents, etc. used in the following examples, unless otherwise specified, are obtained through commercial channels.

[0042] Examples 1-11

[0043] Preparation of the skin care bacteriostatic composition of the present application: each example includes the following ingredients in the weight ratio as shown in Table 1:

[0044] Table 1 Skin care bacteriostatic composition of Examples 1-7

[0045]

[0046] In Example 1, the preparation method of the forsythia suspensa extract is: ultrasonic extraction with 20% v / v ethanol aqueous solution at 30°C: first extract for 25 min at 30 kHz, then extract for 20 min at a frequency of 60 kHz; after the extraction is completed, filter, and reduce the pressure to concentrate the obtained filtrate to 1.25 g / ml, and then obtain; wherein the mass of 20% v / v ethanol aqueous solution is 6 times the mass of forsythia suspensa;

[0047] The preparation method of the honeysuckle extract is: ultrasonic extraction with 55% v / v ethanol aqueous solution at 50°C: first extract for 25 min at 30 kHz, then extract for 18 min at a frequency of 60 kHz; after the extraction is completed, filter, and reduce the pressure to concentrate the obtained filtrate to 1.25 g / ml, and then obtain; wherein the mass of 55% v / v ethanol aqueous solution is 8 times the mass of the honeysuckle;

[0048] The preparation method of the grain extract solution is: ① corn and oat are crushed in a mass ratio of 1.5:1, soaked in water for 22 h, and then the water is drained off. The soaked corn and oat are steamed for 50 min, cooled to 40℃, and then 0.75%, 1.2% and 1.25% of Lactobacillus casei, Lactobacillus bulgaricus and Saccharomyces cerevisiae, respectively, based on the total mass of the corn and oat, are added. After being mixed uniformly, the mixture is fermented at 37-42℃ for 24 h, filtered, and then the obtained filtrate is warmed to reflux and kept at reflux for 45 min to obtain a fermentation liquor; ② soybean is crushed, and 8 times the mass of water is added. The soybean is extracted by microwave at a power of 800 W and a temperature of 40℃ for 12 min. Then 1.35% and 1.15% of lipase and neutral protease, respectively, based on the mass of the soybean, are added. After enzymolysis at 45-55℃ for 2.5 h, the temperature is increased to 93℃ and kept for 8 min to inactivate the enzymes. After filtration, an enzymolysis liquor is obtained; ③ the fermentation liquor obtained in step ① and the enzymolysis liquor obtained in step ② are concentrated to 1.15 g / ml, respectively, to obtain a concentrated fermentation liquor and a concentrated enzymolysis liquor. The concentrated fermentation liquor and the concentrated enzymolysis liquor are mixed in a ratio of 1:1.25 to obtain the grain extract solution.

[0049] In Example 2, the preparation method of the forsythia suspensa extract solution is ultrasonic extraction with 15% v / v ethanol aqueous solution at 25℃: first ultrasonic extraction at 25 kHz for 30 min, and then ultrasonic extraction at a frequency of 50 kHz for 25 min. After the extraction is completed, the obtained filtrate is concentrated to 1.2 g / ml under reduced pressure to obtain the forsythia suspensa extract solution; wherein the mass of the 15% v / v ethanol aqueous solution is 5 times the mass of the forsythia suspensa;

[0050] The preparation method of the honeysuckle extract solution is ultrasonic extraction with 45% v / v ethanol aqueous solution at 45℃: first ultrasonic extraction at 20 kHz for 30 min, and then ultrasonic extraction at a frequency of 55 kHz for 20 min. After the extraction is completed, the obtained filtrate is concentrated to 1.2 g / ml under reduced pressure to obtain the honeysuckle extract solution; wherein the mass of the 45% v / v ethanol aqueous solution is 7 times the mass of the honeysuckle;

[0051] The preparation method of the grain extract solution is: ① corn and oat are crushed in a mass ratio of 1:1, soaked in water for 18 h, and then the water is drained off. The soaked corn and oat are steamed for 45 min, cooled to 37℃, and then 0.5%, 1.5% and 1% of Lactobacillus casei, Lactobacillus bulgaricus and Saccharomyces cerevisiae, respectively, based on the total mass of the corn and oat, are added. After being mixed uniformly, the mixture is fermented at 37-42℃ for 20 h, filtered, and then the obtained filtrate is warmed to reflux and kept at reflux for 30 min to obtain a fermentation liquor; ② soybean is crushed, and 6 times the mass of water is added. The soybean is extracted by microwave at a power of 850 W and a temperature of 35℃ for 15 min. Then 1.5% and 1.3% of lipase and neutral protease, respectively, based on the mass of the soybean, are added. After enzymolysis at 45-55℃ for 2 h, the temperature is increased to 90℃ and kept for 10 min to inactivate the enzymes. After filtration, an enzymolysis liquor is obtained; ③ the fermentation liquor obtained in step ① and the enzymolysis liquor obtained in step ② are concentrated to 1.1 g / ml, respectively, to obtain a concentrated fermentation liquor and a concentrated enzymolysis liquor. The concentrated fermentation liquor and the concentrated enzymolysis liquor are mixed in a ratio of 1:1 to obtain the grain extract solution.

[0052] In Example 3, the preparation method of the forsythia suspense extract solution is ultrasonic extraction with 25% v / v ethanol aqueous solution at 35℃: first ultrasonic extraction at 35 kHz for 20 min, and then ultrasonic extraction at a frequency of 70 kHz for 15 min. After the extraction is completed, the obtained filtrate is concentrated to 1.3 g / ml under reduced pressure to obtain the forsythia suspense extract solution; wherein the mass of the 25% v / v ethanol aqueous solution is 8 times the mass of the forsythia suspense;

[0053] The preparation method of the honeysuckle extract solution is ultrasonic extraction with 65% v / v ethanol aqueous solution at 60℃: first ultrasonic extraction at 35 kHz for 20 min, and then ultrasonic extraction at a frequency of 70 kHz for 15 min. After the extraction is completed, the obtained filtrate is concentrated to 1.3 g / ml under reduced pressure to obtain the honeysuckle extract solution; wherein the mass of the 65% v / v ethanol aqueous solution is 10 times the mass of the honeysuckle;

[0054] The preparation method of the cereal extract solution is: ① corn and oat are crushed in a mass ratio of 2:1, soaked in water for 26 hours, and then the water is drained off, the soaked corn and oat are steamed for 1 hour, cooled to 42 DEG C, and then 1%, 0.8% and 1.5% of Lactobacillus casei, Lactobacillus bulgaricus and Saccharomyces cerevisiae, respectively, of the total mass of corn and oat are added, mixed uniformly, and then fermented at 37-42 DEG C for 26 hours, filtered, heated to reflux, and kept at reflux for 1 hour to obtain a fermentation liquor; ② soybean is crushed, 9 times of water by mass of the soybean is added, and then microwave extraction is carried out at a power of 750 W and a temperature of 45 DEG C for 10 minutes, 1.2% and 1.0% of lipase and neutral protease, respectively, of the mass of the soybean is added, and then enzymolysis is carried out at 45-55 DEG C for 3 hours, and then the temperature is increased to 95 DEG C for 5 minutes to inactivate the enzyme, and then filtered to obtain an enzymolysis liquor; ③ the fermentation liquor obtained in step 1 and the enzymolysis liquor obtained in step 2 are concentrated to 1.2 g / ml respectively to obtain concentrated fermentation liquor and concentrated enzymolysis liquor, and then the concentrated fermentation liquor and the concentrated enzymolysis liquor are mixed in a ratio of 1:1.5 to obtain the cereal extract solution.

[0055] In examples 4-7, the preparation methods of the cereal extract solution, the forsythia suspensa extract solution and the honeysuckle extract solution are the same as those in example 1.

[0056] Examples 8-16

[0057] The present application provides a skin care bacteriostatic solution and a preparation method thereof, which comprises the following components in the weight ratio as shown in Table 2 in each example:

[0058] Table 2: Skin care bacteriostatic solution in examples 8-16

[0059]

[0060]

[0061] The preparation methods of the cereal extract solution, the forsythia suspensa extract solution and the honeysuckle extract solution are the same as those in example 1.

[0062] The preparation method of the skin care bacteriostatic solution is:

[0063] S1, the cereal extract solution, the forsythia suspensa extract solution, the honeysuckle extract solution, phenoxyethanol, benzalkonium chloride and 30% of the formula amount of deionized water are premixed to obtain a premix;

[0064] S2, under the condition of stirring at 50 r / min, cocamidopropyl betaine, lauryl glucoside, decyl glucoside, glycerol and PEG-7 glyceryl cocoate are sequentially added to the remaining formula amount of deionized water, stirred uniformly, heated to 50-60 DEG C, stirred for another 10-25 min, and then vacuum degassed;

[0065] S3, when the material obtained in S2 is cooled to 40-45℃, the premix obtained in S1 is added thereto, stirred uniformly, filtered with a 200-mesh filter screen, and the skin care and bacterium inhibiting solution is obtained.

[0066] Comparative Example 1

[0067] This comparative example provides a skin care and bacterium inhibiting solution and a preparation method thereof, which has the same component ratio and preparation method as Example 8, wherein the preparation method of the grain extract solution is as follows: corn and oat are crushed in a mass ratio of 1.5:1, soaked in water for 22h, and then the water is drained off, the soaked corn and oat are steamed for 50min, cooled to 40℃, and then Lactobacillus casei, Lactobacillus bulgaricus and Saccharomyces cerevisiae corresponding to 0.75%, 1.2% and 1.25% of the total mass of corn and oat are added, mixed uniformly, and then fermented at 37-42℃ for 24h, filtered, and then the obtained filtrate is warmed to reflux and kept at reflux for 45min to obtain a fermentation liquor; the fermentation liquor is concentrated to 1.15g / ml, and then the skin care and bacterium inhibiting solution is obtained.

[0068] The preparation method of the forsythia suspensa extract solution and the honeysuckle extract solution is the same as that in Example 1.

[0069] Comparative Example 2

[0070] This comparative example provides a skin care and bacterium inhibiting solution and a preparation method thereof, which has the same component ratio and preparation method as Example 8, wherein the preparation method of the grain extract solution is as follows: soybean is crushed, 8 times of water corresponding to the mass of the soybean is added, microwave extraction is carried out at a power of 800W and a temperature of 40℃ for 12min, and then lipase and neutral protease corresponding to 1.35% and 1.15% of the mass of the soybean are added, and then enzymolysis is carried out at 45-55℃ for 2.5h, and then the temperature is increased to 93℃ for 8min to inactivate the enzyme, and then the solution is filtered to obtain an enzymolysis solution; the enzymolysis solution is concentrated to 1.15g / ml, and then the skin care and bacterium inhibiting solution is obtained.

[0071] The preparation method of the forsythia suspensa extract solution and the honeysuckle extract solution is the same as that in Example 1.

[0072] Comparative Example 3

[0073] This comparative example provides a skin care and bacterium inhibiting solution and a preparation method thereof, which has the same component ratio and preparation method as Example 8, wherein the preparation method of the forsythia suspensa extract solution is as follows: forsythia suspensa is heated to reflux with 7 times of 55% v / v ethanol aqueous solution for 2 times, each for 30min; the filtrate is obtained by filtering, and then the filtrate is concentrated to 1.25g / ml under reduced pressure, and then the forsythia suspensa extract solution is obtained.

[0074] The preparation method of the grain extract solution and the honeysuckle extract solution is the same as that in Example 1.

[0075] Comparative Example 4

[0076] This comparative example provides a skin care antibacterial liquid and a preparation method thereof. The composition ratio and preparation method of the skin care antibacterial liquid are the same as those of Example 8, wherein the preparation method of the Forsythia suspensa extract is as follows: ultrasonically extracting the Forsythia suspensa with 7 times 20% v / v ethanol aqueous solution for 45 minutes at an ultrasonic frequency of 45 Hz; filtering, and concentrating the filtrate under reduced pressure to 1.25 g / ml to obtain the Forsythia suspensa extract;

[0077] The preparation methods of the cereal extract and the honeysuckle extract are the same as those in Example 1.

[0078] Comparative Example 5

[0079] This comparative example provides a skin care antibacterial liquid and a preparation method thereof. The composition ratio and preparation method of the skin care antibacterial liquid are the same as those of Example 8, wherein the preparation method of the honeysuckle extract is as follows: extracting the honeysuckle with 7 times 57% v / v ethanol aqueous solution by heating and refluxing twice, each time for 30 minutes; filtering, and concentrating the filtrate under reduced pressure to 1.25g / ml to obtain the product;

[0080] The preparation methods of the forsythia extract and the cereal extract are the same as those in Example 1.

[0081] Comparative Example 6

[0082] This comparative example provides a skin care antibacterial liquid and a preparation method thereof. The composition ratio and preparation method of the skin care antibacterial liquid are the same as those of Example 8, wherein the preparation method of the honeysuckle extract is as follows: extracting the honeysuckle with 8 times 55% v / v ethanol aqueous solution by ultrasonication for 45 minutes at an ultrasonic frequency of 45 Hz; filtering, and concentrating the filtrate under reduced pressure to 1.25 g / ml to obtain the product;

[0083] The preparation methods of the forsythia extract and the cereal extract are the same as those in Example 1.

[0084] Comparative Example 7

[0085] This comparative example provides a skin care antibacterial liquid and a preparation method thereof. The composition ratio of the skin care antibacterial liquid is based on Example 8, except for the cereal extract, forsythia extract and honeysuckle extract. The remaining ingredients remain unchanged, and the preparation method is the same as Example 8.

[0086] Test Example 1

[0087] This test example investigated the antibacterial effect of the skin care antibacterial liquids prepared in Examples 8 to 16 and Comparative Examples 1 to 7.

[0088] The composition ratio of the nutrient agar medium used in the following experiments is: peptone 10g, beef extract 3g, sodium chloride 5g, agar 17g, distilled water 1000mL;

[0089] The ingredients of Sabouraud medium are: 10 g peptone, 20 g agar, 40 g glucose, and 1 L distilled water.

[0090] Test strains: Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231).

[0091] 1. Experimental method

[0092] The 4th generation nutrient agar culture (24 h) of the test bacteria was washed with 5 mL of 0.03 mol / L phosphate buffer (hereinafter referred to as PBS) to remove the bacterial mat, diluted with the above PBS, and prepared into a bacterial suspension with a concentration of 5 x 10 4 cfu / mL.

[0093] The skin care bacteriostatic composition samples prepared in Examples 8-16 and Comparative Examples 1-7 were used as the test sample liquid, and 4 tubes of the test sample liquid (5 mL) and the control sample liquid (having the same composition as the test sample liquid, but not containing the grain extract, forsythia extract, honeysuckle extract, phenoxyethanol and benzalkonium chloride, and sterilized) were taken.

[0094] The test was carried out according to GB 15979-2002 "Hygienic Standard for Disposable Hygienic Products" Appendix C, 100 μL of the above bacterial suspension was added dropwise into each of the test sample liquid and the control sample liquid, mixed uniformly, and the timing was started. After 2 min, 5 min, 10 min and 20 min of action, 0.5 mL of the mixed sample was taken with sterile forceps and placed in a test tube containing 5 mL of PBS, mixed thoroughly, and 0.5 mL was taken and placed on two plates. Nutrient agar medium (bacteria) or Sabouraud agar medium (yeast) at 15 mL, cooled to 40-50°C, was poured, the plates were rotated to mix thoroughly, and after the agar was solidified, the plates were inverted and incubated at 35°C ± 2°C for 48 h (bacteria) or 72 h (yeast). The viable bacterial colonies were counted.

[0095] The test was repeated 3 times, and the bacteriostatic rate was calculated according to the following formula:

[0096] X = (A-B) / A x 100%;

[0097] In the formula, X is the bacteriostatic rate, A is the average number of colonies of the control sample liquid, and B is the average number of colonies of the test sample liquid.

[0098] 2. Experimental results

[0099] The average bacteriostatic rate of each skin care bacteriostatic composition at different action times was recorded, and the results are shown in Tables 3-5.

[0100] Table 3 Bacteriostatic data against Staphylococcus aureus

[0101]

[0102] Table 4 Bacteriostatic data against Escherichia coli

[0103]

[0104] Table 5 shows the antibacterial data of Candida albicans

[0105]

[0106]

[0107] As can be seen from the test results in Tables 3-5, the skin care antibacterial compositions obtained in Examples 8-16 have an antibacterial rate of >99.9% for Staphylococcus aureus, Escherichia coli, and Candida albicans at 5-20 min, reaching the standard value specified in Appendix C of GB 15979-2002 "Hygienic Standard for Disposable Hygienic Products", and have strong antibacterial effect. The antibacterial rates of the skin care antibacterial compositions obtained in Examples 8-16 are all better than those of the skin care antibacterial compositions obtained in Comparative Examples 1-7.

[0108] Test Example 2

[0109] This test example investigates the skin irritation of the skin care antibacterial solutions prepared in Examples 8-16 and Comparative Examples 1-7.

[0110] 1. Experimental method

[0111] 63 New Zealand rabbits with intact skin, female, weighing 2.0-2.5 kg, were fed with ordinary grade rabbit maintenance feed at a temperature of 20-26°C and a relative humidity of 40%-70%.

[0112] According to "Hygienic Safety Evaluation Technical Requirements for Disinfectant Products" (WS 628-2018), the skin care antibacterial solutions prepared in Examples 8-16 and Comparative Examples 1-7 were subjected to a complete skin irritation test (3 rabbits were used for each sample): about 24 hours before the experiment, the fur on both sides of the rabbit's back was removed without damaging the skin, and the fur was removed in an area of about 3 cm x 3 cm on both left and right sides. 0.5 ml of the test solution was applied to the skin, then covered with two layers of gauze and one layer of glass paper, and fixed with adhesive tape. The other side of the skin was applied with 0.5 ml of distilled water as a blank control. The application time was 2 hours, and the test material was removed by washing with warm water for 1 min. The skin local reaction was observed at 1 hour, 24 hours, and 48 hours after the test material was removed, and the skin irritation reaction score was evaluated according to Table 6. The results are shown in Tables 7 and 8.

[0113] Table 6 Skin irritation reaction score standard

[0114]

[0115]

[0116] Table 7 Test results of skin care bacteriostatic solution obtained in Examples 8-16

[0117]

[0118]

[0119] Table 8 Test results of skin care bacteriostatic solution obtained in Comparative Examples 1-7

[0120]

[0121] As shown in the results of Table 7 and Table 8, the skin care bacteriostatic solution obtained in Examples 8-16 has no irritation to the skin of white rabbits, while the skin care bacteriostatic solution obtained in Comparative Examples 1-7 has certain irritation to the skin of white rabbits.

[0122] Test Example 3

[0123] Forty-five volunteers aged 22-45 years old with healthy skin were selected and randomly divided into 15 groups, 3 persons in each group. Before the test, the left and right hands were washed and dried, and the volunteers were stabilized in a room with temperature of 22-26°C and relative humidity of 45-55% for 30 minutes. Each group of volunteers was washed the left hand with the skin care bacteriostatic solution prepared in Examples 8-16 and Comparative Examples 1-7 above for 20 seconds (assisted by others), and the water was wiped off, which was the test side. At the same time, the right hand was washed with clean water, and the water was wiped off, which was the control side.

[0124] The skin hydration rate (MMV) of the back of the hands was tested at different times using a skin moisture tester, and the average of 5 measurements was taken as the result, and the MMV change rate was calculated.

[0125] MMV change rate = (skin hydration rate of test side - skin hydration rate of control side) / skin hydration rate of control side x 100%.

[0126] The results are shown in Table 9.

[0127] Table 9 MMV change rate

[0128] Group 1 min after wiping off moisture (%) 60 min after wiping off moisture (%) Example 8 5.76±0.26a 19.28±0.41a Example 9 5.69±0.29a 18.96±0.37a Example 10 5.61±0.24a 18.89±0.34a Example 11 5.74±0.31a 19.12±0.39a Example 12 5.66±0.25a 18.95±0.41a Example 13 5.37±0.27a 18.63±0.36a Example 14 5.68±0.23a 19.02±0.37a Example 15 5.34±0.31a 18.71±0.41a Example 16 5.71±0.27a 19.14±0.38a Comparative Example 1 5.32±0.26a 13.26 ± 0.42 ab Comparative Example 2 5.25±0.30a 13.22 ± 0.40 ab Comparative Example 3 5.29±0.28a 14.39 ± 0.37 ab Comparative Example 4 5.31±0.26a 14.47 ± 0.35 ab Comparative Example 5 5.24±0.29a 14.26 ± 0.38 ab Comparative Example 6 5.27±0.27a 14.35 ± 0.36 ab Comparative Example 7 1.28±0.16 9.67±0.21b

[0129] Note: aP<0.05 compared with Comparative Example 7; bP<0.05 compared with Example 8.

[0130] As shown in the results of Test Examples 1, 2 and 3, the skin care bacteriostatic solution provided by the examples of the present application has excellent bacteriostatic effect, and compared with the comparative examples, the skin care bacteriostatic solution provided by the examples of the present application can more effectively moisturize and nourish the skin, and has better skin care effect.

[0131] The above merely provides the preferred embodiment of the present application, and is not used to limit the present application, and any modification, equivalent replacement or improvement made in the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A skin care and antibacterial composition, characterized in that: The invention is composed of the following ingredients in parts by weight: 0.1-1 part of cereal extract, 0.1-0.5 part of forsythia extract, 0.1-0.5 part of honeysuckle extract, 0.05-0.5 part of phenoxyethanol and 0.01-0.5 part of benzalkonium chloride; The preparation method of the cereal extract comprises the following steps: S1, corn and oats are crushed, soaked in water for 18-26 hours, and water is drained off. The soaked corn and oats are steamed for 45 min-1 hour, cooled to 37-42 ° C, and Lactobacillus casei, Lactobacillus bulgaricus and Saccharomyces cerevisiae are added. After mixing, the mixture is fermented at 37-42 ° C for 20-26 hours, filtered, and the filtrate is heated to reflux and kept at reflux for 0.5-1 hour to obtain a fermentation liquid; the mass ratio of the corn and the oats is 1-2:1; the mass of the Lactobacillus casei is 0.5-1% of the total mass of the corn and oats, the Lactobacillus bulgaricus is 0.8-1.5% of the total mass of the corn and oats, and the Saccharomyces cerevisiae is 1-1.5% of the total mass of the corn and oats; S2. Crush the soybeans, add water equivalent to 6 to 9 times the mass of the soybeans, and microwave extract for 10 to 15 minutes at a power of 750 to 850 W and a temperature of 35 to 45° C., add lipase and neutral protease, and enzymolyze at 45 to 55° C. for 2 to 3 hours, then raise the temperature to 90 to 95° C. and maintain for 5 to 10 minutes to inactivate the enzymes, and filter to obtain an enzymatic hydrolyzate; the mass of the lipase is 1.2 to 1.5% of the mass of the soybeans, and the mass of the neutral protease is 1.0 to 1.3% of the mass of the soybeans; S3. Concentrate the fermentation broth obtained in step S1 and the enzymatic hydrolysate obtained in step S2 to 1.1-1.2 g / ml respectively to obtain concentrated fermentation broth and concentrated enzymatic hydrolysate, and mix the concentrated fermentation broth and concentrated enzymatic hydrolysate in a ratio of 1:1-1.5 to obtain the grain extract.

2. The skin care and antibacterial composition according to claim 1, characterized in that The invention is composed of the following ingredients in parts by weight: 0.2-0.8 parts of cereal extract, 0.1-0.4 parts of forsythia extract, 0.1-0.4 parts of honeysuckle extract, 0.05-0.3 parts of phenoxyethanol and 0.01-0.3 parts of benzalkonium chloride.

3. The skin care and antibacterial composition according to claim 2, characterized in that The invention is composed of the following ingredients in parts by weight: 0.8 parts of cereal extract, 0.4 parts of forsythia extract, 0.4 parts of honeysuckle extract, 0.3 parts of phenoxyethanol and 0.1 parts of benzalkonium chloride.

4. The skin care and antibacterial composition according to any one of claims 1 to 3, characterized in that The preparation method of the Forsythia suspensa extract comprises: subjecting the Forsythia suspensa to ultrasonic extraction with a 15-25% v / v ethanol aqueous solution at 25-35° C., first extracting at a frequency of 25-35 kHz for 20-30 minutes, and then extracting at a frequency of 50-70 kHz for 15-25 minutes; after the extraction is completed, filtering and concentrating the obtained filtrate to 1.2-1.3 g / ml to obtain the Forsythia suspensa extract; wherein the mass of the 15-25% v / v ethanol aqueous solution is 5-8 times the mass of the Forsythia suspensa; and / or The preparation method of the honeysuckle extract is as follows: ultrasonically extracting honeysuckle with a 45-65% v / v ethanol aqueous solution at 45-60° C., first extracting at a frequency of 20-35 kHz for 20-30 minutes, and then extracting at a frequency of 55-70 kHz for 15-20 minutes; after the extraction is completed, filtering and concentrating the obtained filtrate to 1.2-1.3 g / ml to obtain the honeysuckle extract; wherein the mass of the 45-65% v / v ethanol aqueous solution is 7-10 times the mass of the honeysuckle.

5. Use of the skin care and antibacterial composition according to any one of claims 1 to 4 in the preparation of skin care and antibacterial products.

6. A skin care antibacterial liquid, characterized in that: The invention comprises the skin care and antibacterial composition according to any one of claims 1 to 4.

7. The skin care antibacterial liquid according to claim 6, characterized in that The composition comprises the following components in parts by weight: 30-80 parts of deionized water; 1-20 parts of cocamidopropyl betaine; 1-10 parts of lauryl glucoside; 1-10 parts of decyl glucoside; 1-10 parts of glycerin; PEG-7 Glyceryl Cocoate 0.2-0.9 parts; 0.1~1 part of cereal extract; 0.1-0.5 parts of Forsythia suspensa extract; 0.1-0.5 parts of honeysuckle extract; Phenoxyethanol 0.05~0.5 parts; 0.01~0.5 parts of benzalkonium chloride.

8. The method for preparing the skin-protecting antibacterial liquid according to claim 7, characterized in that: The following steps are involved: Premixing the cereal extract, forsythia extract, honeysuckle extract, phenoxyethanol, benzalkonium chloride, and 25-35% of the formula amount of deionized water to obtain a premix; Under stirring, add the cocamidopropyl betaine, lauryl glucoside, decyl glucoside, glycerin and PEG-7 glyceryl cocoate to the remaining amount of deionized water in sequence, stir until completely dissolved and mix, and then vacuum degas; The premix is ​​added to the obtained material, stirred evenly, and filtered to obtain the skin care antibacterial liquid.

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