A circulating tumor cell separation and capture device and method
By designing a cyclic tumor cell separation capture device using micro -flow -controlled chips and air pressure -driven devices, the problem of low CTC separation capture efficiency in existing technologies has been solved. Early detection and personalized therapy of tumor disease.
Patent Information
- Application Number
- CN202011004342.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2020-09-22
- Publication Date
- 2025-01-28
- Estimated Expiration
- 2040-09-22
AI Technical Summary
The prior art has problems of specificity and inefficiency in the isolation and capture of circulating tumor cells (CTCs), especially because tumor cells from different tissue origins unevenly express EpCAM, resulting in limitations of the isolation method.
Design a cyclic tumor cell separation capture device, use micro -flow control chip and air pressure drive device, and collect tubes, waste liquid collection tubes and enrichment liquid collection pipes through pipes to achieve the blood samples in the micro -current control chip Separation and capture. This device forms a pressure difference between different pipes through different air pressure drivers. Driving samples and solutions flow in micro -current control chips to achieve the separation and enrichment of CTC.
The efficient separation and capture of CTC have the advantages of simple structural, automatic operation, low cost, and short time, which can provide high -active CTC cell suspension for multiple analysis methods, improve the early detection and personalization of tumor diseases The ability to treat.
Smart Images
Figure CN114250140B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a circulating tumor cell (CTC) separation and capture device, which drives a blood sample into a microfluidic chip through air pressure control, so that the sample can separate and capture circulating tumor cells (CTC) in the microfluidic chip. Background Art
[0002] At present, the continuous increase in tumor incidence and mortality has become one of the serious problems in today's society. At present, the clinical discovery and diagnosis of most tumors still rely on imaging examinations, traditional tumor markers and tissue biopsies. Traditional invasive tissue biopsies require taking a piece of tissue or cell sample from the patient's body, which often brings certain risks and pain to the patient. More importantly, tissue biopsies are not suitable for repeated extraction and are prone to complications, and have the risk of spreading cancer cells. Related studies have confirmed that in the early stages of cancer, tumors will release circulating tumor cells (CTCs) into the blood. By detecting changes in these markers, tumors can be detected at an early stage. Therefore, the detection and analysis of circulating tumor cells in patients' blood samples is the key to improving the early detection rate of tumor diseases and personalized treatment.
[0003] Since the number of CTCs in patient blood samples is very small, there are only 1-10 CTCs per milliliter of whole blood in patients with tumor metastasis, making CTC detection very challenging. Currently, the CellSearch system is the only CTC detection technology approved by the FDA for clinical use to predict metastatic breast cancer, prostate cancer, and colorectal cancer. This technology separates and captures CTCs by coating the cell surface adhesion molecule EpCAM on magnetic beads. However, since 30% of tumor cells from different tissues do not express EpCAM, and EpCAM is lost due to the possible EMT of tumor cells, the specificity and efficiency of this method are greatly reduced, which greatly limits the downstream CTC detection analysis. Summary of the invention
[0004] The technical problem to be solved by the present invention is to provide a circulating tumor cell (CTC) separation and capture device with a simple structure, stability and reliability.
[0005] The technical solution provided by the present invention is as follows:
[0006] A circulating tumor cell separation and capture device, the separation and capture device comprises a microfluidic chip, the microfluidic chip is sealed and connected with a sample tube, a first waste liquid collection tube, a solution tube, a second waste liquid collection tube and an enriched liquid collection tube; the sample tube is used to provide a sample to be separated to the microfluidic chip; the first waste liquid collection tube is used to collect the main waste liquid after separation by the microfluidic chip; the solution tube is used to provide a secondary treatment solution to the primary enriched liquid sample after the main waste liquid is removed, and the solution is used to dilute the primary enriched liquid sample after the main waste liquid is removed and provide a secondary separation dynamic force; the second waste liquid collection tube is used to collect secondary waste liquid after separation by the microfluidic chip; the enriched liquid collection tube is used to collect the target cell enriched liquid obtained after separation by the microfluidic chip; the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are respectively connected with air pressure driving devices, and the air pressure driving devices respectively introduce air pressure into the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube and form a pressure difference between different tubes to drive the sample to enter the microfluidic chip through the tubular object and flow in the microfluidic chip.
[0007] Furthermore, the air pressure driving device includes a first air pressure driving device connected to the sample tube to drive the sample in the sample tube to enter the microfluidic chip, a second air pressure driving device connected to the first waste liquid collection tube, a third air pressure driving device connected to the solution tube to drive the solution in the solution tube to enter the microfluidic chip, and a fourth air pressure driving device connected to the second waste liquid collection tube and the enriched liquid collection tube, wherein the driving pressure provided by the first air pressure driving device is the largest, and the driving pressure provided by the fourth air pressure driving device is the lowest.
[0008] Furthermore, the driving pressures provided by the air pressure drive devices are as follows: the first air pressure drive device is larger than the second air pressure drive device, and the third air pressure drive device is larger than the fourth air pressure drive device; the air pressure source may be a micro air pump built into the device or an external air source; the air pressure precision control device is an electric proportional valve.
[0009] Furthermore, the sample tube is connected to a first air inlet pipe, and the first air pressure driving device is provided with a first air nozzle movably connected to the first air inlet pipe; the first waste liquid collecting tube is connected to a second air inlet pipe, and the second air pressure driving device is provided with a second air nozzle movably connected to the second air inlet pipe; the solution tube is connected to a third air inlet pipe, and the third air pressure driving device is provided with a third air nozzle movably connected to the third air inlet pipe; the second waste liquid collecting tube and the enriched liquid collecting tube are connected to a fourth forked air inlet pipe, and the fourth air pressure driving device is provided with a fourth air nozzle movably connected to the fourth forked air inlet pipe.
[0010] Furthermore, the tumor cell separation and capture device also includes a card board and a card board fixing seat supporting the card board, the microfluidic chip is arranged on the top of the card board, and the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are arranged on the bottom of the card board, and the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are respectively sealed and connected to the microfluidic chip through a tubular object passing through the card board.
[0011] Furthermore, the circulating tumor cell separation and capture device also includes a base plate, on which a slide rail and a stepper motor are provided, the card plate fixing seat is arranged above the slide rail and moves back and forth on the slide rail through a synchronous belt driven by the stepper motor; the slide rail is provided with a guide rail slider, and the guide rail slider is located between the slide rail and the card plate fixing seat.
[0012] Furthermore, the base plate is provided with an air nozzle fixing seat, and the first air nozzle, the second air nozzle, the third air nozzle and the fourth air nozzle are arranged on the air nozzle fixing seat; the side of the clamping plate fixing seat is provided with a first pair of interfaces, a second pair of interfaces, a third pair of interfaces and a fourth pair of interfaces for respectively movably and sealingly docking the first air inlet pipe, the second air inlet pipe, the third air inlet pipe and the fourth air inlet pipe with the first air nozzle, the second air nozzle, the third air nozzle and the fourth air nozzle.
[0013] Furthermore, the tumor cell separation and capture device includes a shell, an opening is provided on the shell at a position corresponding to the card plate fixing seat, and the card plate fixing seat enters and exits through the opening under the drive of a stepper motor; the shell is also provided with a touch display screen for realizing the control of the separation and capture device and the display of the current operating status.
[0014] A method for separating and capturing circulating tumor cells, the method comprising the following steps:
[0015] (1) Place the blood sample in the sample tube and the solution tube with the solution to be used, then install the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube to the card holder;
[0016] (2) According to the program, the air pressure values of the first air pressure driving device, the second air pressure driving device, the third air pressure driving device, and the fourth air pressure driving device are set and started. Driven by the air pressure provided by the first air pressure driving device, the blood in the sample tube flows through the tubular object into the microfluidic chip. Since the air pressure value set by the first air pressure driving device is greater than the air pressure value set by the second air pressure driving device, and is restricted by the internal structure and flow path of the microfluidic chip, the main waste blood is caused to flow out from the outlet of the first waste liquid collection tube and collected in the first waste liquid collection tube. The blood flow process is also a separation process. The primary enriched liquid sample after removing the main waste blood is collected in the microfluidic chip. The fluidic chip continues to flow along the internal path. Under the action of the air pressure provided by the third air pressure driving device, the solution in the solution tube (in one embodiment, a mixed solution containing PBS is used) flows into the microfluidic chip through the tubular object at the same time, further diluting the primary enriched liquid sample after the main waste blood is removed and providing power for secondary separation. Since the air pressure value set by the third air pressure driving device is greater than the air pressure value set by the fourth air pressure driving device, and is limited by the internal structure and flow path of the microfluidic chip, the secondary waste blood formed by the separation flows into the second waste liquid collection tube, and the target liquid rich in circulating tumor cells flows into the enriched liquid collection tube, completing the separation and capture process.
[0017] Compared with the prior art, the advantages of the present invention are one-step separation, short time, low cost, integrated microfluidic chip, automated operation process, simple structure, stable performance and good reliability. After the blood sample is separated by the device, the cell suspension containing CTC is collected in the collection tube, and the cell activity is high. In addition to being used for immunofluorescence and FISH staining, it can also be applied to digital PCR, single cell culture, gene sequencing and other analyses, providing accurate detection services for tumor patients. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a simple schematic diagram of the present invention;
[0019] Figure 2 It is an internal schematic diagram of the present invention;
[0020] Figure 3 An exploded view of the present invention;
[0021] Figure 4 Schematic diagram of the microfluidic chip of the present invention. DETAILED DESCRIPTION
[0022] The present invention will be further explained and illustrated below in conjunction with the accompanying drawings.
[0023] A circulating tumor cell separation and capture device, the separation device comprising a microfluidic chip (1), the microfluidic chip (1) being sealed and connected to a sample tube (21), a first waste liquid collection tube (22), a solution tube (23), a second waste liquid collection tube (24) and an enrichment liquid collection tube (25), the microfluidic chip (1) and the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enrichment liquid collection tube (25) are all assembled in a detachable manner, so as to be conveniently replaced when separating different samples; generally speaking, the microfluidic chip (1) is disposable, and the various tubes used are preferably centrifuge tubes; the sample tube (21) is used to provide the microfluidic chip (1) with a sample to be separated; the first waste liquid collection tube (22) is used to collect the main waste liquid after separation by the microfluidic chip (1); the solution tube (23) is used to remove the main waste liquid; A secondary treatment solution is provided for the primary enriched liquid sample after the waste liquid is removed, and the solution is used to dilute the primary enriched liquid sample after the main waste liquid is removed and provide power for secondary separation; the second waste liquid collection tube (24) is used to collect the secondary waste liquid after separation by the microfluidic chip (1); the enriched liquid collection tube (25) is used to collect the target cell enriched liquid obtained after separation by the microfluidic chip (1); the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25) are respectively connected to an air pressure driving device (3), and the air pressure driving device respectively introduces air pressure into the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25) to form a pressure difference between different tubes to drive the sample to flow in the microfluidic chip (1).
[0024] Preferably, the air pressure driving device (3) comprises a first air pressure driving device (31) connected to the sample tube (21) for driving the sample in the sample tube (21) to enter the microfluidic chip (1), a second air pressure driving device (32) connected to the first waste liquid collection tube (22), a third air pressure driving device (33) connected to the solution tube (23) for driving the solution in the solution tube (23) to enter the microfluidic chip (1), and a fourth air pressure driving device (34) connected to the second waste liquid collection tube (24) and the enriched liquid collection tube (25), wherein the driving pressure provided by the first air pressure driving device (31) is the largest, and the driving pressure provided by the fourth air pressure driving device (34) is the lowest.
[0025] When in use, a blood sample is placed in the sample tube (21) (the blood sample is first subjected to simple treatment, such as adding a buffer solution of the same volume as the blood), a standby solution is placed in the solution tube (23), the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25) are installed, the air pressure values of the first air pressure driving device (31), the second air pressure driving device (32), the third air pressure driving device (33) and the fourth air pressure driving device (34) are set according to a program and started, and the blood in the sample tube (21) flows into the microfluidic chip (1) through the tubular object under the drive of the air pressure provided by the first air pressure driving device (31). Since the air pressure value set by the first air pressure driving device is greater than the air pressure value set by the second air pressure driving device, and is restricted by the internal structure and flow path of the microfluidic chip (1), the main waste blood is driven from the first waste liquid collection tube (22). The liquid flows out from the outlet of the collecting tube (22) and is collected in the first waste liquid collection tube (22). The flow process is also a separation process. The primary enriched liquid sample after the main waste blood is removed continues to flow along the internal path in the microfluidic chip (1). Under the action of the air pressure provided by the third air pressure driving device (33), the solution in the solution tube (23) (in one embodiment, a mixed solution containing PBS is used) flows into the microfluidic chip (1) through the tubular object at the same time, further diluting the primary enriched liquid sample after the main waste blood is removed and providing power for secondary separation. Since the air pressure value set by the third air pressure driving device (33) is greater than the air pressure value set by the fourth air pressure driving device, and is limited by the internal structure and flow path of the microfluidic chip (1), the secondary waste blood formed by separation flows into the second waste liquid collection tube (24), and the target liquid rich in circulating tumor cells flows into the enriched liquid collection tube (25), completing the separation and capture process.
[0026] In one embodiment, the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24), and the enriched liquid collection tube (25) are connected to the microfluidic chip (1) via a capillary seal, such as Figure 3 shown.
[0027] Furthermore, the driving pressures set for the air pressure drive devices (31 / 32 / 33 / 34) are as follows: the driving pressure of the first air pressure drive device (31) is greater than that of the second air pressure drive device (32), and the driving pressure of the third air pressure drive device (33) is greater than that of the fourth air pressure drive device (34); wherein the driving pressure of the first air pressure drive device (33) is the highest, and the driving pressure of the fourth air pressure drive device (34) is the lowest.
[0028] In one embodiment, the air pressure set for the first air pressure driving device (31) is 380 mbar, the air pressure set for the second air pressure driving device (32) is 275 mbar, the air pressure set for the third air pressure driving device (33) is 280 mbar, and the air pressure set for the fourth air pressure driving device (34) is 0-150 mbar.
[0029] Furthermore, the sample tube (21) is connected to a first air inlet pipe (211), and the first air pressure drive device (31) is provided with a first air nozzle (41) movably connected to the first air inlet pipe (211); the first waste liquid collection tube (22) is connected to a second air inlet pipe (221), and the second air pressure drive device (32) is provided with a second air nozzle (42) movably connected to the second air inlet pipe (221); the solution tube (23) is connected to a third air inlet pipe (231), and the third air pressure drive device (33) is provided with a third air nozzle (43) movably connected to the third air inlet pipe (231); the second waste liquid collection tube (24) and the enriched liquid collection tube (25) are connected to a fourth bifurcated air inlet pipe (241), and the fourth air pressure drive device (34) is provided with a fourth air nozzle (44) movably connected to the fourth bifurcated air inlet pipe (241). The air pressure source of each air pressure driving device (3) can be provided by a micro air pump (35), which can be one or more, or an external air source; the air pressure value of each air pressure driving device (31 / 32 / 33 / 34) can be set and adjusted through an electric proportional valve.
[0030] Furthermore, the circulating tumor cell separation and capture device also includes a cardboard (5) and a cardboard fixing seat (6) supporting the cardboard (5); the microfluidic chip (1) is arranged on the top of the cardboard (5); the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25) are arranged on the bottom of the cardboard (5); the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25) are respectively sealed and connected to the microfluidic chip (1) through a tubular object passing through the cardboard (5).
[0031] In one embodiment, the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24), the enriched liquid collection tube (25) and the clamping plate (5) are connected by threaded connection and can be fixed by rotation during use. This connection method makes replacement more convenient. Of course, in addition to this connection method, other connection methods may also be used, such as snap-on or plug-in connection.
[0032] In one embodiment, the microfluidic chip (1) and the card board (5) are bonded together by a special glue, and the card board (5) is provided with the following Figure 4 The openings shown are used for the capillary to pass through, and each opening corresponds exactly to the blood inlet of the sample tube (21), the waste liquid outlet of the first waste liquid collection tube (22), the solution inlet of the solution tube (23), the waste liquid outlet of the second waste liquid collection tube (24) and the blood outlet of the enriched liquid collection tube (25).
[0033] Furthermore, the tumor cell separation and capture device also includes a base plate (7), on which a slide rail (71) and a stepper motor (72) are provided, and the card holder (6) is arranged above the slide rail (71) and moves back and forth on the slide rail (71) driven by the stepper motor (72) via a synchronous belt (72).
[0034] In one embodiment, Figure 3 As shown, the slide rail (71) is a double rail, and a slot-type photoelectric switch (73) is provided next to one of the slide rails (71).
[0035] In one embodiment, a guide rail slider (711) is provided on the slide rail (71), and the card plate fixing seat (6) slides back and forth on the slide rail (71) more smoothly and steadily with the help of the guide rail slider (711).
[0036] Furthermore, a gas nozzle fixing seat (8) is provided on the bottom plate (7), and the first gas nozzle (41), the second gas nozzle (42), the third gas nozzle (43) and the fourth gas nozzle (44) are arranged on the gas nozzle fixing seat (8); and a first pair of interfaces (51), a second pair of interfaces (52), a third pair of interfaces (53) and a fourth pair of interfaces (54) are provided on the side of the clamping plate fixing seat (6) for the first gas inlet pipe, the second gas inlet pipe, the third gas inlet pipe and the fourth gas inlet pipe to be movably sealed and docked with the first gas nozzle (41), the second gas nozzle (42), the third gas nozzle (43) and the fourth gas nozzle (44) respectively.
[0037] Furthermore, the tumor cell separation and capture device comprises a shell (01), an opening (02) is provided on the shell (01) at a position corresponding to the cardboard fixing seat (6), the cardboard fixing seat (6) moves in and out through the opening (02) driven by the stepping motor (72), and a button (03) for controlling the operation of the stepping motor (72) is provided on the shell (01), when the button (03) is pressed, the stepping motor (72) works, the synchronous belt (72) starts to run, and drives the cardboard fixing seat (6) to move forward and move out of the shell (01), so as to load or unload the sample tube (21), the first waste liquid collection tube (22), the solution tube (23), the second waste liquid collection tube (24) and the enriched liquid collection tube (25), a baffle (61) is provided in front of the cardboard fixing seat (6), and the baffle (61) is connected to the cardboard fixing seat (6) so that it can move in and out simultaneously with the cardboard fixing seat (6), and a transparent window (62) is provided on the baffle (61), through which the separation situation inside the tumor cell separation and capture device can be observed at any time; a touch screen (04) is also provided on the housing (01), and the touch screen (04) is connected to each air pressure driving device (31 / 32 / 33 / 34) through a control component, and is used to set and adjust the driving pressure of each air pressure driving device (31 / 32 / 33 / 34) when using the circulating tumor cell separation and capture device, and to control and monitor the normal operation of the entire device.
Claims
1. A circulating tumor cell separation and capture device, characterized in that: The separation and capture device includes a microfluidic chip, and the microfluidic chip is sealed and connected with a sample tube, a first waste liquid collection tube, a solution tube, a second waste liquid collection tube and an enriched liquid collection tube; the sample tube is used to provide the sample to be separated to the microfluidic chip; the first waste liquid collection tube is used to collect the main waste liquid after separation by the microfluidic chip; the solution tube is used to provide a secondary treatment solution to the primary enriched liquid sample after the main waste liquid is removed, and the solution is used to dilute the primary enriched liquid sample after the main waste liquid is removed and provide power for secondary separation; the second waste liquid collection tube is used to collect the secondary waste liquid after separation by the microfluidic chip; the enriched liquid collection tube is used to collect the target cell enriched liquid obtained after separation by the microfluidic chip; the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are separated The first waste liquid collection tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are connected to the air pressure driving device, and the air pressure driving device respectively introduces air pressure into the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube to form a pressure difference between different tubes to drive the sample to enter the microfluidic chip through the tubular object and flow in the microfluidic chip; the air pressure driving device includes a first air pressure driving device connected to the sample tube to drive the sample in the sample tube to enter the microfluidic chip, a second air pressure driving device connected to the first waste liquid collection tube, a third air pressure driving device connected to the solution tube to drive the solution in the solution tube to enter the microfluidic chip, and a fourth air pressure driving device connected to the second waste liquid collection tube and the enriched liquid collection tube, wherein the driving pressure provided by the first air pressure driving device is the largest, and the driving pressure provided by the fourth air pressure driving device is the lowest; The sample tube is connected to a first air inlet pipe, and the first air pressure drive device is provided with a first air nozzle movably connected to the first air inlet pipe; the first waste liquid collection pipe is connected to a second air inlet pipe, and the second air pressure drive device is provided with a second air nozzle movably connected to the second air inlet pipe; the solution tube is connected to a third air inlet pipe, and the third air pressure drive device is provided with a third air nozzle movably connected to the third air inlet pipe; the second waste liquid collection pipe and the enriched liquid collection pipe are connected to a fourth bifurcated air inlet pipe, and the fourth air pressure drive device is provided with a fourth air nozzle movably connected to the fourth bifurcated air inlet pipe; The tumor cell separation and capture device also includes a cardboard and a cardboard fixing seat supporting the cardboard, the microfluidic chip is arranged on the top of the cardboard, the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are arranged on the bottom of the cardboard, and the sample tube, the first waste liquid collection tube, the solution tube, the second waste liquid collection tube and the enriched liquid collection tube are respectively connected to the microfluidic chip through a tubular object passing through the cardboard; The tumor cell separation and capture device also includes a bottom plate, on which a slide rail and a stepper motor are arranged, the cardboard fixing seat is arranged above the slide rail and moves back and forth on the slide rail through a synchronous belt driven by the stepper motor; a guide rail slider is arranged on the slide rail, and the guide rail slider is located between the slide rail and the cardboard fixing seat; A gas nozzle fixing seat is provided on the bottom plate, and the first gas nozzle, the second gas nozzle, the third gas nozzle and the fourth gas nozzle are arranged on the gas nozzle fixing seat; the side of the cardboard fixing seat is provided with a first pair of interfaces, a second pair of interfaces, a third pair of interfaces and a fourth pair of interfaces for the first air inlet pipe, the second air inlet pipe, the third air inlet pipe and the fourth air inlet pipe to be movably and sealedly connected with the first gas nozzle, the second gas nozzle, the third gas nozzle and the fourth gas nozzle respectively.
2. The circulating tumor cell separation and capture device according to claim 1, characterized in that: The driving pressures provided by the air pressure drive devices are as follows: the first air pressure drive device is larger than the second air pressure drive device, and the third air pressure drive device is larger than the fourth air pressure drive device; the air pressure source is a micro air pump built into the device or an external air source; the air pressure precision control device is an electric proportional valve.
3. The circulating tumor cell separation and capture device according to claim 1, characterized in that: The tumor cell separation and capture device comprises a shell, an opening is arranged on the shell at a position corresponding to the card plate fixing seat, and the card plate fixing seat moves in and out through the opening driven by a stepping motor.
4. The circulating tumor cell separation and capture device according to claim 3, characterized in that: The housing is provided with a touch screen for controlling the separation and capture device and displaying the current operating status.
Citation Information
Patent Citations
Circulating tumor cell separating and capturing device
CN212581899U