Preparation method of triple semi-quantitative ovulation detection reagent

A semi-quantitative detection and reagent technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of single type of reagents, unfavorable storage, and error in results, and achieve the effect of facilitating long-term storage, reducing use errors, and enhancing applicability

CN114252632AActive Publication Date: 2022-03-29金华科生物技术河北有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2022-03-29

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Abstract

The invention discloses a preparation method of a triple semi-quantitative ovulation detection reagent. The preparation method comprises the following steps: step 1, preparing a chloroauric acid aqueous solution; 2, preparing a reducing agent; step 3, preparing a reagent; step 4, centrifugal treatment; step 5, adding a stabilizer; and step 6, packaging and sub-packaging. In the step 3, the dosage of the trisodium citrate aqueous solution of the reagent 1 is changed into 2ml, 3ml or 4ml, and in the step 5, the polymer stabilizer is a natural polymer and is selected from one of protein, cellulose and glucose. Compared with an existing triple semi-quantitative ovulation detection reagent preparation method, the preparation method has the advantages that by adopting a sucrose density gradient centrifugation mode, the yield of the ovulation detection reagent is increased, and the yield of the ovulation detection reagent is increased; colloidal gold particles of the reagent are more uniform in size, and use errors are reduced; the stability of the reagent is improved by adding the high-molecular stable substance, so that the reagent is convenient to store for a long time; according to the invention, the applicability of the kit is enhanced in a manner of preparing three reagents and sub-packaging the reagents.
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Description

technical field

[0001] The invention relates to the technical field of medical detection reagents, in particular to a preparation method of a triple semi-quantitative detection reagent for ovulation. Background technique

[0002] Clinically, ovulation test strips can be used to detect the level of luteinizing hormone in urine during pregnancy preparation. The semi-quantitative test strips mainly use monoclonal immune chromatography technology, which can effectively capture ovulation within 24 hours, which is more accurate than ordinary test strips. Many, specific reagents are needed to make test paper, but the preparation method of the existing triple semi-quantitative detection ovulation reagent, the obtained reagent colloidal gold particle size is not uniform, prone to result errors; the existing triple semi-quantitative detection ovulation reagent The preparation method of the obtained reagent is prone to deposition, which is not conducive to long-term storage; the prepar...

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Embodiment Construction

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0019] see figure 1 , an embodiment provided by the present invention: a preparation method of a triple semi-quantitative ovulation detection reagent, comprising the following steps: Step 1, making an aqueous solution of chloroauric acid; Step 2, making a reducing agent; Step 3, preparing the reagent; Step 4 , centrifugation; step five, adding stabilizers; step six, packaging and subpackaging;

[0020] Wherein in the above step 1, put a certain amount of gold...