Electromagnetic composite biomacromolecule separation technology and equipment

Abstract

The present invention discloses an electromagnetic composite biomacromolecule separation technology, which divides the gel in the electrophoresis tank into several segmented areas in parallel along the material separation direction, each segmented area has an independent electric field and magnetic field; the electric field and magnetic field in all segmented areas are controllable; the electric field and magnetic field work together to drive the biomacromolecules to move, and ensure that the movement directions of the biomacromolecules in all segmented areas are parallel. The present invention adds a magnetic field generator in the electrophoresis tank, and uses the characteristics of the magnetic field acting on the movement of biomacromolecules to achieve linear movement during the separation of biomacromolecules without directional deviation. The design is reasonable, the structure is simple, and the actual use effect is good, which creates a precedent for the use of magnetic fields in the separation of biomacromolecules.

Description

Technical Field

[0001] The present invention relates to the field of biomacromolecule separation technology, and in particular to an electromagnetic composite biomacromolecule separation technology and equipment. Background Art

[0002] Electrophoresis tanks are often used in biomedical biomacromolecule separation experiments. The principle is that the terminals of the electrophoresis tank include positive and negative electrodes, and the positive and negative electrodes are connected to the positive and negative output terminals of the DC power supply respectively. The anode electrode inside the electrophoresis tank, various gels with a spatial grid steric structure, the cathode electrode in the electrophoresis tank, the conductive buffer solution between the cathode end of the immersed gel and the cathode electrode of the electrophoresis tank, and the anode end of the immersed gel and the anode electrode of the electrophoresis tank form a DC circuit loop.

[0003] The mixture of biological macromolecules (DNA or RNA or protein) of different molecular weights in the negative ion state after treatment is powered by the DC electric field of the electrophoresis tank, and migrates from the cathode end to the anode end of the electrophoresis tank in the gel with molecular sieve effect. The larger the molecule, the greater the migration resistance in the gel. Under the same electrophoresis starting point and electrophoresis time, the separation of mixed biological macromolecules is achieved. Common electrophoresis tanks on the market include horizontal electrophoresis devices, vertical electrophoresis devices and tubular electrophoresis devices.

[0004] There are many drawbacks in the existing electrophoresis methods and equipment. The positive and negative electrodes in the electrophoresis tank 1 of the existing various electrophoresis methods and equipment are only fixedly distributed at the two ends of the gel 3, and the positive and negative electrodes at the output end of the DC power supply are also fixed. In this way, at the far ends of both sides of the entire gel, the electric field movement direction 4 of the biomacromolecules 2 is in an eight-shaped direction, and the direction has a certain tilt angle, such as Figure 1 The outward-facing movement direction will lead to incomplete separation and may cause leakage and other unexpected situations.

[0005] With the study of biomagnetism, it is found that biological macromolecules themselves also have certain magnetic material properties, which are manifested as biological paramagnetism under certain conditions. Biological paramagnetism plays an important role in biological energy and physiological functions in the biological function activities of proteins and enzymes. From this, it can be known that under the action of a magnetic field, biological macromolecules can achieve sequential movement.

[0006] It can be seen that the combination of the two technologies can be used to separate biomacromolecules. In view of this, the existing electrophoresis technology needs to be further improved. Summary of the invention

[0007] In view of the shortcomings of the prior art, the present invention provides an electromagnetic composite biomacromolecule separation technology and equipment to solve the defects of the above-mentioned technology.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] An electromagnetic composite biomacromolecule separation technology, characterized in that: the gel in the electrophoresis tank is divided into several segmented areas in parallel along the material separation direction, each segmented area has an independent electric field and magnetic field; the electric field and magnetic field in all segmented areas are controllable; the electric field and magnetic field work together to drive the biomacromolecules to move, and ensure that the movement directions of the biomacromolecules in all segmented areas are parallel.

[0010] A further optimized technical solution is characterized in that the electric field is each segmented electric field output by the same DC power supply through a plurality of terminal assemblies, and the terminal assemblies correspond one-to-one to the segmented areas; the magnetic field is a combined magnetic field, including a plurality of magnetic field generating assemblies corresponding one-to-one to the segmented areas; the magnetic field generating assemblies and the terminal assemblies are electrically connected to the same DC power supply.

[0011] A further optimized technical solution, the magnetic field generating component includes an induction coil and a magnetic field conductor arranged in the induction coil; the induction coil includes a metal coil and a current regulator and a switch connected in series with the metal coil; the switch and the current regulator are respectively connected to the two poles of the DC power supply.

[0012] A further optimized technical solution is that the magnetic field generating component includes one induction coil or two induction coils; when the number is one induction coil, the induction coil is installed on the electrophoresis tank corresponding to one end of the segmented area; when the number is two induction coils, the two induction coils are installed on the electrophoresis tanks corresponding to both ends of the segmented area, and the directions of the magnetic fields are consistent.

[0013] According to a further optimized technical solution, all the induction coils are the same, and the number of turns and winding direction are the same.

[0014] The present invention also provides an electromagnetic composite biomacromolecule separation device, which is characterized in that: it includes a shell, an electrophoresis tank is installed in the shell; a control box is installed on the outer surface of the shell, and a power switch and a power relay are installed in the control box; an electric field generator and a magnetic field generator are installed on the shell; a plate is installed in the electrophoresis tank, a gel is installed on the plate, and the plate and the gel are divided into several segmented areas; the electric field generator and the magnetic field generator correspond to all the segmented areas; the power relay and the power switch are connected to an external DC power supply, and the power relay is electrically connected to the electric field generator and the magnetic field generator.

[0015] A further optimized technical solution, the electric field generator includes a plurality of terminal assemblies installed inside the shell and corresponding one-to-one to the segmented areas, and a positive power connector and a negative power connector installed outside the shell; the positive power connector and the negative power connector are connected to the power relay, and the positive power connector and the negative power connector are connected to the terminal assemblies.

[0016] A further optimized technical solution, the magnetic field generator includes a magnetic field relay, a branch wire bank and a plurality of magnetic field generating components, all of the magnetic field generating components correspond one-to-one to all the segmented areas; the power relay is connected to the magnetic field relay, the magnetic field relay is connected to the branch wire bank, and the branch wire bank is connected to the magnetic field generating components.

[0017] A further optimized technical solution, wherein a magnetic field generating component includes an induction coil and a magnetic field conductor arranged in the induction coil; the induction coil includes a metal coil and a current regulator and a switch connected in series with the metal coil; the switch and the current regulator are respectively connected to the branch bank.

[0018] According to a further optimized technical solution, the layers of the composite paperboard are connected together by a hot pressing process.

[0019] Due to the adoption of the above technology, compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. The present invention adds a magnetic field generator in the electrophoresis tank. The electric field generator generates an electric field. Biomacromolecules are essentially charged biomolecules, which migrate under the action of the electric field. The biomacromolecules themselves also have certain magnetism. The magnetic field generated by the magnetic field generator will also cause the biomacromolecules to migrate under the action of the magnetic field. The two cooperate with each other to ensure that the biomacromolecules maintain linear motion during the separation process without directional deviation.

[0021] 2. The present invention has reasonable design and good practical effect, and has pioneered the use of magnetic fields in the separation of biomacromolecules.

[0022] 3. The present invention also designs a set of equipment, which realizes the separation of biomacromolecules by the dual effects of electric field and magnetic field under the same DC power supply, thereby improving the separation accuracy of large molecular weight biomacromolecules. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the movement of electrophoretic separation of biomacromolecules under the existing technology;

[0024] Figure 2 A schematic diagram of the movement of biomacromolecule separation under the combined action of electric field and magnetic field in one embodiment of the present invention;

[0025] Figure 3This is a schematic diagram of the external structure of an electromagnetic composite biomacromolecule separation device in the present invention;

[0026] Figure 4 A schematic diagram of the structure of an induction coil in an embodiment of the present invention;

[0027] Figure 5 Schematic diagram of the internal structure of a magnetic field generator in one embodiment of the present invention. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0029] Example 1

[0030] An electromagnetic composite biomacromolecule separation technology, the simplest technology is to directly add a strong electromagnetic field in the electrophoresis tank of the existing electrophoresis equipment, using a large magnetic field generating component, such as Figure 2 , 3 As shown, the magnetic field generating assembly includes an induction coil 14 and a magnetic field conductor 13 disposed in the induction coil 14. The induction coil includes a metal coil and a current regulator 12 and a switch 15 connected in series with the metal coil. The switch 15 and the current regulator 12 are respectively connected to two poles of an external DC power supply.

[0031] The gel 3 in the electrophoresis tank and the electric field generating device are both existing technologies and will not be described in detail in this embodiment.

[0032] The electric field generated by direct current is a special substance that exists in the space around the electric charge and the changing magnetic field. This substance is different from ordinary physical objects. Although it is not composed of molecular atoms, it is an objectively existing special substance. The magnetic field is a special substance that cannot be seen or touched. The magnetic field is not composed of atoms or molecules, but it exists objectively. The magnetic field has the radiation characteristics of waves and particles. Within a certain period of time, electricity can generate magnetism, and magnetism can also bring electricity. The changing electric field and the changing magnetic field constitute an inseparable unified field, which is the electromagnetic field, and the propagation of the changing electromagnetic field in space forms electromagnetic waves. In the present invention, the magnetic field can affect the electric field, ensure that the electromagnetic field is evenly distributed in the segmented area of ​​the gel, ensure the linear motion of the biomacromolecules, and will not appear outward.

[0033] When in use, the electric field movement direction 4 of the biomacromolecule 2 is along the electric field direction, and synchronously, the magnetic field movement direction 5 of the biomacromolecule 2 also works synchronously, and the electric field direction and the magnetic field direction are the same. The two work together to achieve linear movement of the biomacromolecule along one direction without deviation, thereby ensuring the separation effect and improving the separation accuracy of large molecular weight biomacromolecules.

[0034] This embodiment describes the preliminary use of the combined action of electric and magnetic fields, which is forward-looking but not universal.

[0035] The present invention adds a magnetic field generator in the electrophoresis tank. The electric field generator generates an electric field. Biomacromolecules are essentially charged biomolecules, which migrate under the action of the electric field. The biomacromolecules themselves also have certain magnetism. The magnetic field generated by the magnetic field generator will also cause the biomacromolecules to migrate under the action of the magnetic field. The two cooperate with each other to ensure that the biomacromolecules maintain linear motion during separation and will not have directional deviation.

[0036] The invention has reasonable design and good practical use effect, and has created a precedent for using magnetic field in separation of biological macromolecules.

[0037] Example 2

[0038] like Figure 2 , Figure 4 As shown, an electromagnetic composite biomacromolecule separation technology has been greatly improved in the existing equipment, which is more practical and has better effect. The specific improvement is: the gel in the electrophoresis tank 1 is divided into several segmented areas in parallel along the material separation direction, and each segmented area has an independent electric field and magnetic field. The electric field and magnetic field in all segmented areas are controllable; the electric field and magnetic field work together to drive the biomacromolecules to move, and ensure that the movement direction of the biomacromolecules in all segmented areas is parallel.

[0039] The electric field is each segmented electric field outputted by the same DC power supply through a plurality of terminal assemblies, and the terminal assemblies correspond one to one with the segmented areas. The magnetic field is a combined magnetic field, including a plurality of magnetic field generating assemblies corresponding one to one with the segmented areas; the magnetic field generating assemblies and the terminal assemblies are electrically connected to the same DC power supply. The magnetic field generating assembly includes an induction coil 14 and a magnetic field conductor 13 arranged in the induction coil 14. The induction coil 14 includes a metal coil and a current regulator 12 and a switch 15 connected in series with the metal coil, and the switch 15 and the current regulator 12 are respectively connected to the two poles of the DC power supply. In this embodiment, all the induction coils are the same, and the number of turns and winding direction are the same.

[0040] The gel 3 in the electrophoresis tank and the electric field generating device are both existing technologies, and will not be described in detail in this embodiment. In this embodiment, the gel part is used in sections, which ensures a more precise separation.

[0041] When in use, the electric field movement direction 4 of the biomacromolecule 2 is along the electric field direction, and synchronously, the magnetic field movement direction 5 of the biomacromolecule 2 also works synchronously, and the electric field direction and the magnetic field direction are the same. Under the cooperation of the two, the movement directions of the biomacromolecules in all segmented areas are parallel, and there will be no deviation in the movement direction, thereby ensuring the separation effect and improving the separation accuracy of large molecular weight biomacromolecules.

[0042] The present invention adds a magnetic field generator in the electrophoresis tank. The electric field generator generates an electric field. Biomacromolecules are essentially charged biomolecules, which migrate under the action of the electric field. The biomacromolecules themselves also have certain magnetism. The magnetic field generated by the magnetic field generator will also cause the biomacromolecules to migrate under the action of the magnetic field. The two cooperate with each other to ensure that the biomacromolecules maintain linear motion during separation and will not have directional deviation.

[0043] The invention has reasonable design and good practical use effect, and has created a precedent for using magnetic field in separation of biological macromolecules.

[0044] Example 3

[0045] This embodiment is based on the embodiment 2, and the magnetic field generating components are optimized as needed.

[0046] When other technologies remain unchanged, the magnetic field generating component includes one induction coil or two induction coils.

[0047] When the magnetic force of the magnetic field is small, there is one induction coil, which is installed on the electrophoresis tank corresponding to one end of the segmented area.

[0048] When a large magnetic force is required, two induction coils are provided, and the two induction coils are installed on the electrophoresis tanks corresponding to the two ends of the segmented area, and the magnetic field directions are consistent. All the induction coils are the same, and the number of turns and winding directions are the same.

[0049] When in use, the electric field movement direction 4 of the biomacromolecule 2 is along the electric field direction, and synchronously, the magnetic field movement direction 5 of the biomacromolecule 2 also works synchronously, and the electric field direction and the magnetic field direction are the same. Under the cooperation of the two, the movement directions of the biomacromolecules in all segmented areas are parallel, and there will be no deviation in the movement direction, thereby ensuring the separation effect and improving the separation accuracy of large molecular weight biomacromolecules.

[0050] This embodiment adds a magnetic field generator at one or both ends of the electrophoresis tank, and utilizes the characteristics of the magnetic field on the movement of biomacromolecules to achieve linear motion during the separation of biomacromolecules without directional deviation. It has a reasonable design, a simple structure, and good practical effect, creating a precedent for the use of magnetic fields in the separation of biomacromolecules.

[0051] Example 4

[0052] On the basis of Example 3, an electromagnetic composite biomacromolecule separation device is specially designed to perform the joint operation of electric field and magnetic field to achieve the separation of biomacromolecules.

[0053] The present invention also provides an electromagnetic composite biomacromolecule separation device, comprising a shell 7, in which an electrophoresis tank 1 is installed. A control box 11 is installed on the outer surface of the shell 7, and a power switch and a power relay are installed in the control box 11. An electric field generator and a magnetic field generator 9 are installed on the shell 7. A label 10 is provided on the magnetic field generator 9 for easy marking and convenient viewing by staff. A plate body is installed in the electrophoresis tank 1, and a gel 3 is installed on the plate body, and the plate body and the gel 3 are divided into several segmented areas. The electric field generator and the magnetic field generator correspond to all the segmented areas. The power relay and the power switch are connected to an external DC power supply, and the power relay is electrically connected to the electric field generator and the magnetic field generator.

[0054] The electric field generator includes a plurality of terminal assemblies installed inside the housing and corresponding to the segmented areas one by one, and a positive power connector 8 and a negative power connector installed outside the housing. The positive power connector 8 and the negative power connector are connected to the power relay, and the positive power connector and the negative power connector are connected to the terminal assembly. The terminal assembly is an existing technology, which can generate an electric field, realize electrophoresis, and drive the movement of biomacromolecules.

[0055] The magnetic field generator 16 includes a magnetic field relay 17, a branch line 18 and a plurality of magnetic field generating components, and all the magnetic field generating components correspond to all the segmented areas one by one. The power relay is connected to the magnetic field relay 17, the magnetic field relay 17 is connected to the branch line 18, and the branch line 18 is connected to the magnetic field generating component. The magnetic field generating component includes an induction coil 14 and a magnetic field conductor 13 arranged in the induction coil 14. The induction coil 14 includes a metal coil and a current regulator 12 and a switch 15 connected in series with the metal coil. The switch 15 and the current regulator 12 are respectively connected to the branch line.

[0056] The present invention adds a magnetic field generator in the electrophoresis tank. The electric field generator generates an electric field. Biomacromolecules are essentially charged biomolecules, which migrate under the action of the electric field. The biomacromolecules themselves also have certain magnetism. The magnetic field generated by the magnetic field generator will also cause the biomacromolecules to migrate under the action of the magnetic field. The two cooperate with each other to ensure that the biomacromolecules maintain linear motion during separation and will not have directional deviation.

[0057] The invention has reasonable design and good practical use effect, and has created a precedent for using magnetic field in separation of biological macromolecules.

[0058] The present invention also designs a set of equipment, which realizes the separation of biomacromolecules by the dual effects of electric field and magnetic field under the same DC power supply, thereby improving the separation accuracy of large molecular weight biomacromolecules.

[0059] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An electromagnetic composite biomacromolecule separation method, characterized in that: The gel in the electrophoresis tank is divided into a plurality of segmented areas, each of which is distributed along the substance separation direction, the plurality of segmented areas are parallel to each other, and each segmented area has an independent electric field and magnetic field; The electric and magnetic fields in all segmented areas are controllable; The electric field and the magnetic field work together to drive the biomacromolecules to move, and ensure that the movement directions of the biomacromolecules in all segmented areas are parallel; The electric field movement direction of the biomacromolecule is along the electric field direction, and the magnetic field movement direction of the biomacromolecule also works synchronously, and the electric field direction and the magnetic field direction are the same. The cooperation of the two enables the biomacromolecule to move linearly in one direction; The electric field is each segmented electric field output by the same DC power supply through a plurality of terminal assemblies, and the terminal assemblies correspond one-to-one to the segmented areas; the magnetic field is a combined magnetic field, including a plurality of magnetic field generating assemblies corresponding one-to-one to the segmented areas; the magnetic field generating assemblies and the terminal assemblies are electrically connected to the same DC power supply.

2. The electromagnetic composite biomacromolecule separation method according to claim 1, characterized in that: The magnetic field generating component comprises an induction coil and a magnetic field conductor arranged in the induction coil; the induction coil comprises a metal coil and a current regulator and a switch connected in series with the metal coil; the switch and the current regulator are respectively connected to the two poles of a DC power supply.

3. The electromagnetic composite biomacromolecule separation method according to claim 1, characterized in that: The magnetic field generating component comprises one induction coil or two induction coils; When the number is one induction coil, the induction coil is installed on the electrophoresis tank corresponding to one end of the segmented area; When there are two induction coils, the two induction coils are installed on the electrophoresis tanks corresponding to the two ends of the segmented area, and the directions of the magnetic fields are consistent.

4. The electromagnetic composite biomacromolecule separation method according to claim 1, characterized in that: All the induction coils are identical, with the same number of turns and winding direction.

5. An electromagnetic composite biomacromolecule separation device for implementing the electromagnetic composite biomacromolecule separation method according to any one of claims 1 to 4, characterized in that: It comprises a housing, in which an electrophoresis tank is installed; A control box is installed on the outer surface of the shell, and a power switch and a power relay are installed in the control box; an electric field generator and a magnetic field generator are installed on the shell; A plate is installed in the electrophoresis tank, a gel is installed on the plate, and the plate and the gel are divided into several segmented areas; the electric field generator and the magnetic field generator correspond to all the segmented areas; The power relay and the power switch are connected to an external DC power source, and the power relay is electrically connected to the electric field generator and the magnetic field generator.

6. The electromagnetic composite biomacromolecule separation device according to claim 5, characterized in that: The electric field generator includes a plurality of terminal assemblies installed inside the shell and corresponding to the segmented areas one by one, and a positive power connector and a negative power connector installed outside the shell; the positive power connector and the negative power connector are connected to the power relay, and the positive power connector and the negative power connector are connected to the terminal assemblies.

7. The electromagnetic composite biomacromolecule separation device according to claim 6, characterized in that: The magnetic field generator includes a magnetic field relay, a branching line and a plurality of magnetic field generating components, and all the magnetic field generating components correspond to all the segmented areas one by one; the power relay is connected to the magnetic field relay, the magnetic field relay is connected to the branching line, and the branching line is connected to the magnetic field generating components.

8. The electromagnetic composite biomacromolecule separation device according to claim 7, characterized in that: The magnetic field generating component comprises an induction coil and a magnetic field conductor arranged in the induction coil; the induction coil comprises a metal coil and a current regulator and a switch connected in series with the metal coil; the switch and the current regulator are respectively connected to the tapping wire bank.

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