A fully automatic sample injection mechanism for flow cytometer detection

Abstract

The present invention provides a fully automatic sample introduction mechanism for flow cytometer detection, and relates to the technical field of sample introduction mechanism. The fully automatic sample introduction mechanism for flow cytometer detection comprises a detection box, a conveying mechanism is fixedly arranged inside the detection box, a plurality of storage seats are fixedly installed on the conveying mechanism, sample test tubes are movably embedded on the storage seats, a detection mechanism is fixedly connected inside the detection box, and a pushing mechanism is arranged on one side of the detection mechanism. The fully automatic sample introduction mechanism for flow cytometer detection drives the storage seats and the sample test tubes in the storage seats into the interior of the detection box through the conveying mechanism, forming a semi-automatic transportation and feeding work for the sample test tubes. During the operation of the conveying mechanism, a semi-automatic linkage detection work for the sample test tubes is formed through the linkage relationship of the top plate, the push plate, the movable tooth plate, the gear shaft, the fixed tooth plate, the detector and the detection rod, with a high degree of automation, which is convenient for sampling and detection.

Description

Technical Field

[0001] The invention relates to the technical field of sample injection mechanisms, in particular to a full-automatic sample injection mechanism for flow cytometer detection. Background Art

[0002] Flow cytometry is a device for automatic analysis and sorting of cells. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquids, and can sort out designated cell subpopulations according to a pre-selected parameter range.

[0003] The existing fully automatic sample introduction mechanism of flow cytometer has a low degree of automation, a complex structure, a high cost, and inaccurate positioning, which can easily cause data errors during sampling and testing.

[0004] Therefore, it is extremely necessary to design a fully automatic sample introduction mechanism for flow cytometer detection to solve the above problems. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention provides a fully automatic sample introduction mechanism for flow cytometer detection, which solves the problems of low degree of automation, complex structure of the sample introduction mechanism, high cost and data errors during sampling and detection proposed in the above background technology through the linkage relationship between a top plate, a push plate, a movable tooth plate, a gear shaft, a fixed tooth plate, a detector and a detection rod.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a fully automatic sample introduction mechanism for flow cytometer detection, comprising a detection box, a conveying mechanism is fixedly arranged inside the detection box, a plurality of storage seats are fixedly installed on the conveying mechanism, sample test tubes are movably embedded on the storage seats, and push plates are fixedly connected to the positions of the conveying mechanism near the storage seats;

[0009] A detection mechanism is fixedly connected to the inside of the detection box, and the detection mechanism includes a detector. A detection rod is fixedly installed at the bottom of the detector. The detection rod and the storage seat are located on the same vertical plane. A gear shaft is arranged on the top of the detector. A fixed tooth plate is fixedly connected to one side of the detector, and a movable tooth plate is slidably connected to the other side of the detector. Both the movable tooth plate and the fixed tooth plate are meshed with the gear shaft. A pushing mechanism is arranged on one side of the detection mechanism.

[0010] Preferably, both sides of the detection box are provided with openings, and both ends of the conveying mechanism extend to both sides of the detection box through the two openings.

[0011] Preferably, a positioning rod is fixedly connected to one side of the detection rod, and a scraping ring is fixedly connected to one end of the positioning rod close to the detection rod, and the scraping ring is movably sleeved on the bottom of the detection rod and is arranged in close contact with the outer wall of the detection rod.

[0012] Preferably, connecting plates are provided on both sides of the gear shaft, the tops of the two connecting plates extend to the top inner wall of the detection box and are fixedly connected to the detection box, and the gear shaft is rotatably connected to the two connecting plates.

[0013] Preferably, the pushing mechanism comprises a top plate, and mutually matching inclined surfaces are respectively provided on one side of the bottom of the top plate and one side of the top of the push plate, and the top plate and the push plate are located on the same vertical plane.

[0014] Preferably, the bottom of the movable tooth plate is provided with an abutment portion bent toward one side of the top plate, and the abutment portion and the top plate are located on the same vertical plane.

[0015] Preferably, a movable plate is fixedly connected to one side of the bottom of the top plate, one side of the movable plate is slidably connected to the inner wall of the detection box, a fixed plate is arranged above the movable plate and the fixed plate is fixedly connected to the inner wall of the detection box, a spring is arranged between the fixed plate and the movable plate, and both ends of the spring are respectively fixedly connected to the fixed plate and the movable plate.

[0016] The present invention provides a fully automatic sample injection mechanism for flow cytometer detection, which has the following beneficial effects:

[0017] 1. The conveying mechanism drives the storage seat and the sample tube in the storage seat into the interior of the detection box, forming a semi-automatic transportation and feeding work for the sample tube. During the operation of the conveying mechanism, through the linkage relationship between the top plate, the push plate, the movable tooth plate, the gear shaft, the fixed tooth plate, the detector and the detection rod, a semi-automatic linkage detection work for the sample tube is formed, which has a high degree of automation and is convenient for sampling and detection;

[0018] 2. By movably sleeved the scraper ring on the detection rod, after the detection is completed, the detection rod is moved up, and the scraper ring is used to scrape the surface of the detection rod, so that the detection sample attached to the detection rod can be scraped off, avoiding the detection sample from the previous detection work remaining on the detection rod, which is beneficial to the next detection work and improves the accuracy of the sample detection data. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2It is a schematic diagram of the internal structure of the detection box of the present invention;

[0021] Figure 3 It is a front cross-sectional structural schematic diagram of the present invention;

[0022] Figure 4 For the present invention Figure 2 Enlarged view of point A in the middle.

[0023] In the figure:

[0024] 1. Test box;

[0025] 2. Conveying mechanism;

[0026] 3. Storage seat;

[0027] 4. Sample test tube;

[0028] 5. Push the board;

[0029] 6. Pushing mechanism; 61. Fixed plate; 62. Movable plate; 63. Spring; 64. Top plate;

[0030] 7. Detector; 71. Detection rod; 72. Movable tooth plate; 73. Abutment portion; 74. Fixed tooth plate; 75. Gear shaft; 76. Connecting plate;

[0031] 8. Positioning rod; 81. Scraper ring. DETAILED DESCRIPTION

[0032] In the description of the present invention, unless otherwise clearly specified and limited, the terms "set", "install", "connect", "connect", and "fix" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] The standard parts used in the present invention can all be purchased from the market, and the special-shaped parts can all be customized according to the description and the drawings.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0035] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0036] The embodiment of the present invention provides a fully automatic sample introduction mechanism for flow cytometer detection, such as Figure 1-4 As shown, it comprises a detection box 1, a conveying mechanism 2 is fixedly arranged inside the detection box 1, a plurality of storage seats 3 are fixedly installed on the conveying mechanism 2, a sample test tube 4 is movably embedded on the storage seat 3, and a push plate 5 is fixedly connected to the position of the conveying mechanism 2 near the storage seat 3;

[0037] A detection mechanism is fixedly connected inside the detection box 1, and the detection mechanism includes a detection instrument 7. A detection rod 71 is fixedly installed at the bottom of the detection instrument 7. The detection rod 71 and the storage seat 3 are located on the same vertical plane. A gear shaft 75 is arranged on the top of the detection instrument 7. A fixed tooth plate 74 is fixedly connected to one side of the detection instrument 7, and a movable tooth plate 72 is slidably connected to the other side of the detection instrument 7. Both the movable tooth plate 72 and the fixed tooth plate 74 are meshed with the gear shaft 75. A pushing mechanism 6 is arranged on one side of the detection mechanism.

[0038] Furthermore, both sides of the detection box 1 are provided with openings, and both ends of the conveying mechanism 2 extend to both sides of the detection box 1 through the two openings;

[0039] Since openings are provided on both sides of the detection box 1, the two ends of the conveying mechanism 2 can extend to the outside of the two sides of the detection box 1 through the openings, so that in actual use, the loading work can be carried out on one side of the detection box 1, and the unloading work can be carried out on the other side of the detection box 1;

[0040] Since a plurality of storage seats 3 are fixedly connected to the conveying mechanism 2, and the plurality of storage seats 3 are evenly distributed at equal intervals, the storage seats 3 are provided for placing sample test tubes 4. In actual operation, the sample test tubes 4 can be manually inserted into the storage seats 3 on one side of the detection box 1, and the sample test tubes 4 after the test are taken out on the other side of the detection box 1, or all the sample test tubes 4 can be fixedly installed on the storage seats 3 respectively, and the test samples are placed in the sample test tubes 4 on one side of the detection box 1, and the sample test tubes 4 after the test are taken out from the sample test tubes 4 on the other side of the detection box 1, and the sample test tubes 4 are cleaned. According to actual use requirements, the use method can be reasonably selected, and the storage seats 3 and the sample test tubes 4 in the storage seats 3 can be driven into the interior of the detection box 1 by the conveying mechanism 2, so as to form a semi-automatic transportation and feeding operation for the sample test tubes 4.

[0041] Furthermore, a positioning rod 8 is fixedly connected to one side of the detection rod 71, and a scraper ring 81 is fixedly connected to one end of the positioning rod 8 close to the detection rod 71, and the scraper ring 81 is movably sleeved on the bottom of the detection rod 71 and is arranged in close contact with the outer wall of the detection rod 71;

[0042] Since a detection rod 71 is arranged at the bottom of the detector 7, the detection rod 71 is arranged to detect the detection sample placed in the sample test tube 4, and then the detector 7 and the detection rod 71 can be controlled to move downward, so that the detection rod 71 is embedded in the interior of the sample test tube 4, and then the detection rod 71 contacts the detection sample in the sample test tube 4, and the detection sample is detected and analyzed by the detector 7;

[0043] After the detection is completed, the detection instrument 7 and the detection rod 71 are controlled to reset. Since the scraper ring 81 is movably connected to the detection rod 71, and the scraper ring 81 is fixedly connected to the detection box 1 through the positioning rod 8, that is, the position of the scraper ring 81 is fixed, and the detection rod 71 moves inside the scraper ring 81. Therefore, after the detection is completed, the detection rod 71 moves up, and the scraper ring 81 scrapes the surface of the detection rod 71, so that the detection sample attached to the detection rod 71 can be scraped off, avoiding the detection sample from the last detection work to remain on the detection rod 71, which is beneficial to the next detection work. In addition, the detection instrument 7 and the detection rod 71 accurately affect the data of the detection sample, thereby improving the accuracy of the detection data of the sample.

[0044] Furthermore, connecting plates 76 are provided on both sides of the gear shaft 75. The tops of the two connecting plates 76 extend to the top inner wall of the detection box 1 and are fixedly connected to the detection box 1. The gear shaft 75 is rotatably connected to the two connecting plates 76.

[0045] A gear shaft 75 is provided at the top of the detector 7, and the gear shaft 75 is fixedly connected to the detection box 1 through two connecting plates 76, and the gear shaft 75 and the connecting plate 76 are rotatably connected, and the gear shaft 75 is respectively meshed with the movable tooth plate 72 and the fixed tooth plate 74, the movable tooth plate 72 is slidably connected to one side of the detector 7, and the fixed tooth plate 74 is fixed to one side of the detector 7. In actual installation, a positioning column can be provided on one side of the fixed tooth plate 74, and the fixed tooth plate 74 slides with the positioning column and is connected to the inner wall of the detection box 1 through the positioning column. When the external environment applies an upward thrust to the movable tooth plate 72, the movable tooth plate 72 moves up and causes the gear shaft 75 to rotate. The rotation of the gear shaft 75 forces the fixed tooth plate 74 to move down. As a result, when the movable tooth plate 72 moves up, the fixed tooth plate 74 drives the detector 7 and the detection rod 71 at the bottom of the detector 7 to move down until the detection rod 71 is embedded in the sample tube 4 on the conveying mechanism 2 opposite to its bottom, and then the detection rod 71 contacts the detection sample in the sample tube 4, and the detection sample is detected and analyzed by the detector 7.

[0046] Further, the pushing mechanism 6 includes a top plate 64, and one side of the bottom of the top plate 64 and one side of the top of the push plate 5 are respectively provided with mutually adapted inclined surfaces, the top plate 64 and the push plate 5 are located on the same vertical plane, and the bottom of the movable tooth plate 72 is provided with an abutment portion 73 bent toward one side of the top plate 64, and the abutment portion 73 and the top plate 64 are located on the same vertical plane, and one side of the bottom of the top plate 64 is fixedly connected to the movable plate 62, and one side of the movable plate 62 is slidably connected to the inner wall of the detection box 1, and a fixed plate 61 is provided above the movable plate 62 and the fixed plate 61 is fixedly connected to the inner wall of the detection box 1, and a spring 63 is provided between the fixed plate 61 and the movable plate 62, and the two ends of the spring 63 are respectively fixedly connected to the fixed plate 61 and the movable plate 62;

[0047] The push plate 5 is fixedly connected to the position near the storage seat 3 on the conveying mechanism 2. Since the storage seat 3, the sample test tube 4 placed in the storage seat 3 and the push plate 5 move with the conveying mechanism 2, when the conveying mechanism 2 is started, the multiple storage seats 3 and the push plate 5 on the conveying mechanism 2 will reciprocate into the interior of the detection box 1. Since the bottom side of the top plate 64 and the top side of the push plate 5 are respectively provided with mutually adapted inclined surfaces;

[0048] The top plate 64 and the push plate 5 are located on the same vertical plane, so when the push plate 5 moves to the bottom of the top plate 64, the top plate 64 will be pushed upward during the movement of the push plate 5, that is, the top plate 64 drives the movable plate 62 to move upward and squeezes the spring 63 between the fixed plate 61 and the movable plate 62. When the top plate 64 moves upward, it will touch the abutment 73 and squeeze the abutment 73, so that the abutment 73 and the movable tooth plate 72 are pushed upward. In actual use, the top of the top plate 64 can also be fixedly connected to the bottom of the abutment 73.

[0049] The movable tooth plate 72 moves upward, causing the gear shaft 75 to rotate, and the rotation of the gear shaft 75 will force the fixed tooth plate 74 to move downward, so that when the movable tooth plate 72 moves upward, the fixed tooth plate 74 drives the detector 7 and the detection rod 71 at the bottom of the detector 7 to move downward until the detection rod 71 is embedded in the sample test tube 4 on the conveying mechanism 2 directly facing the bottom thereof. The rotation speed of the conveying mechanism 2, the upward movement speed of the abutment portion 73 and the movable tooth plate 72, and the downward movement speed of the detector 7 and the detection rod 71 are pre-calculated so that when the push plate 5 pushes the top plate 64 to move upward to the maximum height, the detection rod 71 is just completely embedded in the sample test tube 4 that the electrical concept push plate 5 wants to approach, and then the detection rod 71 contacts the detection sample in the sample test tube 4, and the detection sample is detected and analyzed by the detector 7. Under the coordinated use of the conveying mechanism 2 and the pushing mechanism 6, a semi-automatic linkage detection work of the sample test tube 4 is formed;

[0050] When the push plate 5 is disengaged from the top plate 64, that is, the push plate 5 is not in contact with the top plate 64, the reaction force of the spring 63 pushes the movable plate 62 downward, so that the movable plate 62 drives the top plate 64 downward, thereby resetting the top plate 64, and waiting for the next push plate 5 on the conveying mechanism 2 to touch the top plate 64, and thus completing the sampling and testing of the sample tubes 4 in the remaining storage seats 3, with a high degree of automation and convenient sampling.

[0051] The specific implementation mode is as follows: in actual use of the present invention, the sample test tube 4 with the test sample can be manually inserted into the storage seat 3 on one side of the test box 1, and the sample test tube 4 after the test is completed can be taken out on the other side of the test box 1. After the sample test tube 4 with the test sample is placed, the conveying mechanism 2 is started, and the storage seat 3 and the sample test tube 4 in the storage seat 3 are driven into the interior of the test box 1 by the conveying mechanism 2;

[0052] The positions of the conveying mechanism 2 near the storage seat 3 are all fixedly connected with the push plate 5. Since the storage seat 3, the sample test tube 4 placed in the storage seat 3 and the push plate 5 are moved with the conveying mechanism 2, when the conveying mechanism 2 is started, the multiple storage seats 3 and the push plate 5 on the conveying mechanism 2 will reciprocate into the interior of the detection box 1. Since the bottom side of the top plate 64 and the top side of the push plate 5 are respectively provided with mutually adaptable inclined surfaces, and the top plate 64 and the push plate 5 are located on the same vertical plane, when the push plate 5 moves to the bottom of the top plate 64, the top plate 64 will be pushed upward during the movement of the push plate 5, that is, the top plate 64 drives the movable plate 62 to move upward and squeezes the spring 63 between the fixed plate 61 and the movable plate 62. When the top plate 64 moves upward, it will touch the abutment 73 and squeeze the abutment 73, so that the abutment 73 and the movable tooth plate 72 are pushed upward;

[0053] The movable tooth plate 72 moves upward, causing the gear shaft 75 to rotate, and the rotation of the gear shaft 75 will force the fixed tooth plate 74 to move downward, so that when the movable tooth plate 72 moves upward, the fixed tooth plate 74 drives the detector 7 and the detection rod 71 at the bottom of the detector 7 to move downward until the detection rod 71 is embedded in the sample test tube 4 on the conveying mechanism 2 directly facing the bottom thereof. The rotation speed of the conveying mechanism 2, the upward movement speed of the abutment portion 73 and the movable tooth plate 72, and the downward movement speed of the detector 7 and the detection rod 71 are pre-calculated so that when the push plate 5 pushes the top plate 64 to move upward to the maximum height, the detection rod 71 is just completely embedded in the sample test tube 4 that the electrical concept push plate 5 wants to approach, and then the detection rod 71 contacts the detection sample in the sample test tube 4, and the detection sample is detected and analyzed by the detector 7. Under the coordinated use of the conveying mechanism 2 and the pushing mechanism 6, a semi-automatic linkage detection work of the sample test tube 4 is formed;

[0054] When the push plate 5 is out of contact with the top plate 64, that is, the push plate 5 is not in contact with the top plate 64, the reaction force of the spring 63 pushes the movable plate 62 downward, that is, the top plate 64 is reset, and the top of the top plate 64 is fixedly connected to the abutment portion 73, so that the movable plate 62 drives the top plate 64 to move downward, and the top plate 64 moves downward, which also causes the movable tooth plate 72 to move downward, and the movable tooth plate 72 moves downward to drive the gear shaft 75 to rotate, forcing the fixed tooth plate 74 to drive the detector 7 and the detector 7 low or high detection rod 71 to move upward;

[0055] The scraper ring 81 scrapes the surface of the detection rod 71, so that the detection sample attached to the detection rod 71 can be scraped off. After the top plate 64 is reset, wait for the next push plate 5 on the conveying mechanism 2 to touch the top plate 64, and then complete the sampling and testing of the sample tubes 4 in the remaining storage seats 3. The degree of automation is high and sampling is convenient.

[0056] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A fully automatic sample introduction mechanism for flow cytometer detection, comprising a detection box (1), characterized in that: A conveying mechanism (2) is fixedly arranged inside the detection box (1), a plurality of storage seats (3) are fixedly mounted on the conveying mechanism (2), sample test tubes (4) are movably embedded on the storage seats (3), and push plates (5) are fixedly connected to positions of the conveying mechanism (2) near the storage seats (3); a detection mechanism is fixedly connected to the inside of the detection box (1); The detection mechanism comprises a detector (7), and a pushing mechanism (6) is arranged on one side of the detection mechanism. The pushing mechanism (6) comprises a top plate (64), and mutually matching inclined surfaces are respectively provided on a bottom side of the top plate (64) and a top side of the push plate (5). When the push plate (5) moves, the top plate (64) is also pushed upward, thereby driving the detector (7) to move downward.

2. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 1, characterized in that: A detection rod (71) is fixedly mounted at the bottom of the detector (7); the detection rod (71) and the storage seat (3) are located on the same vertical plane; a gear shaft (75) is provided at the top of the detector (7); and a fixed toothed plate (74) is fixedly connected to one side of the detector (7).

3. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 2, characterized in that: A movable tooth plate (72) is slidably connected to the other side of the detector (7), and both the movable tooth plate (72) and the fixed tooth plate (74) are meshingly connected to the gear shaft (75).

4. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 1, characterized in that: Both sides of the detection box (1) are provided with openings, and both ends of the conveying mechanism (2) extend to both sides of the detection box (1) through the two openings.

5. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 2, characterized in that: A positioning rod (8) is fixedly connected to one side of the detection rod (71), and a scraper ring (81) is fixedly connected to one end of the positioning rod (8) close to the detection rod (71). The scraper ring (81) is movably sleeved on the bottom of the detection rod (71) and is arranged to fit the outer wall of the detection rod (71).

6. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 3, characterized in that: Connecting plates (76) are provided on both sides of the gear shaft (75); the tops of the two connecting plates (76) extend to the top inner wall of the detection box (1) and are fixedly connected to the detection box (1); the gear shaft (75) is rotatably connected to the two connecting plates (76).

7. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 3, characterized in that: The top plate (64) and the push plate (5) are located on the same vertical plane.

8. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 3, characterized in that: The bottom of the movable tooth plate (72) is provided with an abutment portion (73) bent toward one side of the top plate (64); the abutment portion (73) and the top plate (64) are located on the same vertical plane.

9. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 7, characterized in that: A movable plate (62) is fixedly connected to one side of the bottom of the top plate (64), one side of the movable plate (62) is slidably connected to the inner wall of the detection box (1), and a fixed plate (61) is provided above the movable plate (62) and the fixed plate (61) is fixedly connected to the inner wall of the detection box (1).

10. The fully automatic sample introduction mechanism for flow cytometer detection according to claim 9, characterized in that: A spring (63) is provided between the fixed plate (61) and the movable plate (62), and two ends of the spring (63) are respectively fixedly connected to the fixed plate (61) and the movable plate (62).

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