ROV-Based High-Throughput In-Situ Fixation Device and Method for Deep-Sea Large Organisms

Abstract

The present invention belongs to the field of large-scale in-situ biological equipment, and specifically relates to a deep-sea large-scale biological high-throughput in-situ fixation device and method based on an ROV. A plurality of high-throughput in-situ fixation barrels are placed in a movable frame body. The high-throughput barrel bottoms of each high-throughput in-situ fixation barrel are detachably connected to the movable frame body, and the high-throughput barrel lids of each high-throughput in-situ fixation barrel are switchably connected to the high-throughput barrel bodies. Each high-throughput in-situ fixation barrel is filled with a fixing solution. An oil cylinder is installed on the installation bottom plate. After the movable frame body is placed on the installation bottom plate, the output end of the oil cylinder abuts against the movable frame body to fix the movable frame body. The present invention cooperates with an ROV manipulator to squeeze and crush large organisms and immerse them in the fixing solution in the high-throughput barrel body, and the sample is recovered by using the sealing function of the high-throughput barrel body during the carrying process. The present invention is less affected by the sampling depth, has strong corrosion resistance, a large storage volume, is convenient to carry, and can quickly and effectively fix large biological samples.

Description

Technical Field

[0001] The present invention belongs to the field of large-scale in-situ biological equipment, and specifically relates to a deep-sea large-scale biological high-throughput in-situ fixation device and method based on ROV. Background Art

[0002] Due to the huge differences between the deep-sea environment and the upper ocean environment, during the sampling process, due to the long time, there will be huge changes in pressure and temperature, and the physiological state of organisms after reaching the shore will change greatly. The existing sampling methods cannot truly reproduce the real state of the deep sea, which is the main bottleneck restricting deep-sea biological research.

[0003] Currently, many domestic marine research institutions have carried out research and development on related fixation equipment; however, most of the research on deep-sea biological fixation technologies carried out focuses on microorganisms or simple storage mechanisms for large organisms. For example, in-situ automatic enrichment and fixation devices for microorganisms, in-situ detection devices for primary productivity of microorganisms in extreme deep-sea environments, etc. There are relatively few fixation devices for large organisms. The "Cutting-type In-situ Fixation Device for Large Organisms such as Deep-sea Mussels Based on ROV" announced on August 11, 2020, with the announcement number CN211235013U, and the "Squeezing-type In-situ Fixation Device for Large Organisms such as Deep-sea Mussels Based on ROV" announced on July 17, 2020, with the announcement number CN211042749U have certain involvements, but they cannot fix high-throughput samples. Summary of the Invention

[0004] Referring to the advantages of traditional deep-sea microorganism in-situ enrichment and fixation and combining the characteristics of in-situ fixation methods for large organisms such as deep-sea mussels, the purpose of the present invention is to provide a deep-sea large-scale biological high-throughput in-situ fixation device and method based on ROV. The present invention utilizes the precise positioning and capture ability and observation ability of ROV (Remotely Operated Vehicle), greatly reducing the difficulty of the implementation method.

[0005] The purpose of the present invention is achieved through the following technical solutions:

[0006] The high-throughput in-situ fixation device of the present invention includes a mounting base plate, a moving frame body, and high-throughput in-situ fixation barrels. A plurality of high-throughput in-situ fixation barrels are placed in the moving frame body. The high-throughput barrel bottom of each high-throughput in-situ fixation barrel is detachably connected to the moving frame body, and the high-throughput barrel cover of each high-throughput in-situ fixation barrel is switchably connected to the high-throughput barrel body. Each high-throughput in-situ fixation barrel is filled with a fixing solution; an oil cylinder is installed on the mounting base plate. After the moving frame body is placed on the mounting base plate, the output end of the oil cylinder abuts against the moving frame body to fix the moving frame body.

[0007] Wherein: a bottom plate frame is fixedly connected to the edge of the mounting bottom plate, the oil cylinder is fixed at the rear end of the mounting bottom plate, and a front baffle is provided on the bottom plate frame at the front end of the mounting bottom plate; a lower edge plate is fixedly connected to the bottom of the moving frame body, the output end of the oil cylinder is connected with a blocking plate for squeezing the moving frame body, the height of the blocking plate is higher than that of the lower edge plate, and when the oil cylinder pushes the moving frame body, the front baffle and the blocking plate are respectively connected with the lower edge plate by locking buckles to fix the moving frame body.

[0008] The mounting bottom plate is a square flat plate and is placed on the fixed tray of the ROV; the moving frame body is a square frame, and the square size is smaller than that of the mounting bottom plate. A lifting handle is arranged inside the moving frame body to facilitate moving the moving frame body out of the mounting bottom plate.

[0009] Fixing holes are formed at the bottom of the moving frame body corresponding to the positions of each high-throughput in-situ fixing barrel. Internal threads are formed inside the fixing holes. A central hole is formed in the high-throughput barrel bottom of the high-throughput in-situ fixing barrel. The high-throughput in-situ fixing barrel is connected to the moving frame body through fixing and sealing bolts. One end of the fixing and sealing bolt is hermetically connected to the central hole in the high-throughput barrel bottom, and the other end of the fixing and sealing bolt is threadedly connected to the fixing hole.

[0010] Fixing holes are formed at the bottom of the moving frame body corresponding to the positions of each high-throughput in-situ fixing barrel. Internal threads are formed inside the fixing holes. Grooves are formed in the high-throughput barrel bottom of the high-throughput in-situ fixing barrel, and internal threads are formed inside the grooves. The high-throughput in-situ fixing barrel is connected to the moving frame body through double-headed bolts, and both ends of the double-headed bolts are threadedly connected to the fixing holes and the grooves respectively.

[0011] One end of the high-throughput barrel cover is hinged to the high-throughput barrel body, and a twisting handle is provided at the other end. A rotating rod is provided on the twisting handle. The rotating rod passes through the other end of the high-throughput barrel cover and is rotatably connected to the other end of the high-throughput barrel cover. A rotating stop block located below the high-throughput barrel cover is provided at the end of the rotating rod; a locking piece is provided on the high-throughput barrel body, and a locking hook is fixedly connected to the locking piece. When the high-throughput barrel cover is closed, the rotating stop block rotates to the locking hook along with the twisting handle to lock the high-throughput barrel cover.

[0012] A clamp is sleeved on the high-throughput barrel body, and one end of the high-throughput barrel cover is hinged to the clamp; locking pieces are fixedly connected to both ends of the opening of the clamp, and the two locking pieces are locked and fixed through a locking bolt. A locking hook is welded to the upper end of any one of the locking pieces.

[0013] A fixed lock block A is fixedly connected to the upper surface of one end of the high-throughput barrel cover, and a fixed lock block B is fixedly connected to the clamp. The fixed lock block A is connected to the fixed lock block B through a rotating shaft.

[0014] The top of the high-throughput barrel body is provided with a sealing rubber sheet for sealing the fixing liquid, and there is a slit on the sealing rubber sheet.

[0015] The high-throughput in-situ fixation method for deep-sea large organisms based on ROV of the present invention uses the high-throughput in-situ fixation device for deep-sea large organisms based on ROV described in any one of claims 1 to 9, and includes the following steps:

[0016] Step 1, overall disassembly and cleaning work at the shore-based end. Disassemble and disassemble the high-throughput in-situ fixation device for deep-sea large organisms based on ROV as a whole, and then clean it.

[0017] Step 2, assemble after cleaning. In the initial state, the fixing liquid is stored in the high-throughput barrel body. Rotate the twisting handle, drive the rotating block to rotate to the locking hook through the rotating rod, lock and close the high-throughput barrel cover; place the moving frame into the mounting base plate, the ROV starts the hydraulic power, the oil cylinder acts, uses the baffle plate to squeeze the moving frame, and locks the moving frame on the mounting base plate through the lower edge plate of the front baffle lock.

[0018] Step 3, after being brought to the seabed by the ROV system, relax the oil cylinder, the baffle plate disengages from the moving frame, and use the manipulator to hold the lifting handle and take it away from the ROV.

[0019] Step 4, rotate the twisting handle, drive the rotating block to disengage from the locking hook through the rotating rod, and open the high-throughput barrel cover; use the manipulator to grab the large organism. During the grabbing process, the shell or epidermis of the large organism will be damaged, causing the large organism to be damaged.

[0020] Step 5, use the manipulator to pass the large organism through the sealing rubber sheet and place it in the high-throughput barrel body, soak the large organism with the fixing liquid inside the high-throughput barrel body to fix the large organism; press the twisting handle, close the high-throughput barrel cover, and then rotate the twisting handle, drive the rotating block to rotate to the locking hook through the rotating rod, and lock the high-throughput barrel cover.

[0021] Step 6, repeat Step 4 and Step 5 to complete the operations of multiple high-throughput in-situ fixation barrels.

[0022] Step 7, use the manipulator to hold the lifting handle and put it into the ROV. The ROV starts the hydraulic power, the oil cylinder acts, uses the baffle plate to squeeze the moving frame, and locks the moving frame on the mounting base plate through the lower edge plate of the front baffle lock.

[0023] Step 8, use the ROV to bring back the high-throughput in-situ fixation device for deep-sea large organisms based on ROV.

[0024] Step 9, after recovery, relax the oil cylinder, the baffle plate disengages from the moving frame, open the high-throughput bucket cover, and obtain the sample.

[0025] The advantages and positive effects of the present invention are as follows:

[0026] 1. The present invention meets the in-situ fixation requirements of large organisms such as mussels in the deep-sea ecosystem, is less affected by sampling depth, has strong corrosion resistance, and a large storage volume.

[0027] 2. The present invention is based on the ROV system and can make use of the precise positioning, real-time observation and other characteristics of the ROV.

[0028] 3. The structure of the present invention is simple and easy to implement. Description of the Drawings

[0029] Figure 1 is a schematic three-dimensional structure diagram of the high-throughput in-situ fixation device of the present invention;

[0030] Figure 2 is a top view of the structure of the high-throughput in-situ fixation device of the present invention;

[0031] Figure 3 is a front view of the structure of the high-throughput in-situ fixation device of the present invention;

[0032] Figure 4 is a top view of the structure of the mounting base plate in the high-throughput in-situ fixation device of the present invention;

[0033] Figure 5 is a schematic three-dimensional structure diagram of the high-throughput in-situ fixation device of the present invention after removing the mounting base plate;

[0034] Figure 6 is a top view of the structure of the mounting base plate and the moving frame of the high-throughput in-situ fixation device of the present invention;

[0035] Figure 7 is one of the schematic three-dimensional structure diagrams of the high-throughput in-situ fixation barrel in the high-throughput in-situ fixation device of the present invention;

[0036] Figure 8 is the other schematic three-dimensional structure diagram of the high-throughput in-situ fixation barrel in the high-throughput in-situ fixation device of the present invention;

[0037] Figure 9 is a front view of the structure of the high-throughput in-situ fixation barrel in the high-throughput in-situ fixation device of the present invention;

[0038] Figure 10 is a right view of the structure of the high-throughput in-situ fixation barrel in the high-throughput in-situ fixation device of the present invention;

[0039] Wherein: 1 is the installation base plate, 2 is the base plate frame, 3 is the movable frame body, 4 is the high-throughput in-situ fixing barrel, 5 is the lifting handle, 6 is the front baffle, 7 is the oil cylinder, 8 is the blocking plate, 9 is the lower edge plate, 10 is the fixing hole, 11 is the fixing seal bolt, 12 is the twisting handle, 13 is the rotating rod, 14 is the rotating stop block, 15 is the fixing lock block A, 16 is the rotating shaft, 17 is the fixing lock block B, 18 is the clamp, 19 is the locking piece, 20 is the locking bolt, 21 is the locking hook, 22 is the high-throughput barrel cover, 23 is the high-throughput barrel body, 24 is the high-throughput barrel bottom, and 25 is the sealing rubber sheet. Detailed implementation mode

[0040] The present invention will be further described in detail below with reference to the accompanying drawings.

[0041] As Figures 1 to 10 shown, the high-throughput in-situ fixing device of the present invention includes an installation base plate 1, a movable frame body 3 and a high-throughput in-situ fixing barrel 4. A plurality of high-throughput in-situ fixing barrels 4 are placed in the movable frame body 3. The high-throughput barrel bottom 24 of each high-throughput in-situ fixing barrel 4 is detachably connected to the movable frame body 3. The high-throughput barrel cover 22 of each high-throughput in-situ fixing barrel 4 is switchably connected to the high-throughput barrel body 23. Each high-throughput in-situ fixing barrel 4 is filled with a fixing liquid; an oil cylinder 7 is installed on the installation base plate 1. After the movable frame body 3 is placed on the installation base plate 1, the output end of the oil cylinder 7 abuts against the movable frame body 3 to fix the movable frame body 3.

[0042] The installation base plate 1 of this embodiment is a square flat plate, which is placed on the fixed tray of the ROV; the four peripheral edges of the installation base plate 1 are welded with a base plate frame 2. The oil cylinder 7 is fixed at the rear end of the installation base plate 1. A front baffle 6 is provided on the base plate frame 2 at the front end of the installation base plate 1; the oil circuit power system of the oil cylinder 7 is provided by the ROV, and the output end of the oil cylinder 7 is connected with a blocking plate 8 for extruding the movable frame body 3.

[0043] The movable frame body 3 of this embodiment is a square frame, and the square size is smaller than that of the installation base plate 1. The specific size of the movable frame body 3 is based on the principle of convenient operation of the ROV manipulator; the movable frame body 3 is an independent unit, and a lifting handle 5 is provided inside to facilitate the removal of the movable frame body 3 from the installation base plate 1. The bottom of the movable frame body 3 is fixedly connected with a lower edge plate 9, and the lower edge plate 9 is flush with the bottom of the movable frame body 3. The height of the blocking plate 8 is higher than that of the lower edge plate 9. When the oil cylinder 7 pushes the movable frame body 3, the front baffle 6 and the blocking plate 8 are respectively connected and locked with the lower edge plate 9 to fix the movable frame body 3; that is, the front baffle 6 extends towards the movable frame body 3 and overlaps with the lower edge plate 9.

[0044] At the bottom of the moving frame body 3, fixing holes 10 are provided corresponding to the positions of each high-throughput in-situ fixing barrel 4. Internal threads are made inside the fixing holes 10. A central hole is opened on the high-throughput barrel bottom 24 of the high-throughput in-situ fixing barrel 4. The high-throughput in-situ fixing barrel 4 is connected to the moving frame body 3 through a fixing and sealing bolt 11. One end of the fixing and sealing bolt 11 is provided with a sealing ring, which is hermetically connected to the central hole on the high-throughput barrel bottom 24, and the other end of the fixing and sealing bolt 11 is threadedly connected to the fixing hole 10. Or, at the bottom of the moving frame body 3, fixing holes 10 are provided corresponding to the positions of each high-throughput in-situ fixing barrel 4. Internal threads are made inside the fixing holes 10. A groove is opened on the high-throughput barrel bottom 24 of the high-throughput in-situ fixing barrel 4, and internal threads are made inside the groove. The high-throughput in-situ fixing barrel 4 is connected to the moving frame body 3 through a double-headed bolt, and both ends of the double-headed bolt are threadedly connected to the fixing hole 10 and the groove respectively. In this embodiment, the high-throughput barrel bottom 24 of the high-throughput in-situ fixing barrel 4 is connected to the fixing hole 10 at the bottom of the moving frame body 3 through a fixing and sealing bolt 11.

[0045] In this embodiment, the high-throughput in-situ fixing barrel 4 is divided into a high-throughput barrel cover 22, a high-throughput barrel body 23 and a high-throughput barrel bottom 24. One end of the high-throughput barrel cover 22 is hinged to the high-throughput barrel body 23, and a twisting handle 12 is provided at the other end. A rotating rod 13 is provided on the twisting handle 12. The rotating rod 13 passes through the other end of the high-throughput barrel cover 22 and is rotatably connected to the other end of the high-throughput barrel cover 22. A rotating stop block 14 located below the high-throughput barrel cover 22 is provided at the end of the rotating rod 13. The twisting handle 12, the rotating rod 13 and the rotating stop block 14 are integrated; A locking piece 19 is provided on the high-throughput barrel body 23, and a locking hook 21 is fixedly connected to the locking piece 19. When the high-throughput barrel cover 22 is closed, the rotating stop block 14 rotates to the locking hook 21 along with the twisting handle 12 to realize the locking of the high-throughput barrel cover 22.

[0046] In this embodiment, a clamp 18 is sleeved on the high-throughput barrel body 23, and one end of the high-throughput barrel cover 22 is hinged to the clamp 18; Locking pieces 19 are fixedly connected to both ends of the opening of the clamp 18, and the two locking pieces 19 are locked and fixed through a locking bolt 20. A locking hook 21 is welded to the upper end of any one of the locking pieces 19. On the upper surface of one end of the high-throughput barrel cover 22 in this embodiment, a fixed lock block A 15 is welded, and a fixed lock block B 17 is welded on the clamp 18. The fixed lock block A 15 is connected to the fixed lock block B 17 through a rotating shaft 16. The height position of the clamp 18 on the high-throughput barrel body 23 depends on the tightness when the high-throughput barrel cover 22 is closed.

[0047] At the top of the high-throughput barrel body 23 in this embodiment, a sealing rubber sheet 25 for sealing the fixing liquid is provided. There is a slit on the sealing rubber sheet 25, which can not only seal the fixing liquid, but also place large organisms into the barrel.

[0048] In this embodiment, both the lifting handle 5 and the twisting handle 12 are "T" shaped.

[0049] The present invention relates to a method for high-throughput in-situ fixation of deep-sea large organisms based on an ROV, and uses a device for high-throughput in-situ fixation of deep-sea large organisms based on an ROV. The fixing solution uses the formula of the fixing solution described in The stabilising solution was prepared in the following proportions (US patent 8178296 B2), and includes the following steps:

[0050] Step 1: Overall disassembly and cleaning work at the shore-based end. Disassemble and remove the entire device for high-throughput in-situ fixation of deep-sea large organisms based on an ROV, and then clean it;

[0051] Step 2: Assemble after cleaning. In the initial state, the fixing solution is stored in the high-throughput barrel 23. Rotate the twisting handle 12, and drive the rotating block 14 to rotate to the locking hook 21 through the rotating rod 13 to lock and close the high-throughput barrel cover 22; Place the moving frame 3 into the installation base plate 1. The ROV starts the hydraulic power, and the oil cylinder 7 acts. Use the blocking plate 8 to squeeze the moving frame 3, and lock the moving frame 3 on the installation base plate 1 through the lower edge plate 9 of the front baffle 6 lock;

[0052] Step 3: After being brought to the seabed by the ROV system, relax the oil cylinder 7, the blocking plate 8 disengages from the moving frame 3, and use the manipulator to hold the lifting handle 5 and take it away from the ROV;

[0053] Step 4: Rotate the twisting handle 12, drive the rotating block 14 to disengage from the locking hook 21 through the rotating rod 13, and open the high-throughput barrel cover 22; Use the manipulator to grab large organisms. During the grabbing process, the outer shell or epidermis of the large organisms will be damaged, causing the large organisms to be damaged;

[0054] Step 5: Use the manipulator to pass the large organisms through the sealing rubber 25 and place them in the high-throughput barrel 23, and soak the large organisms with the fixing solution inside the high-throughput barrel 23 to fix the large organisms; Press the twisting handle 12 to close the high-throughput barrel cover 22, and then rotate the twisting handle 12, drive the rotating block 14 to rotate to the locking hook 21 through the rotating rod 13, and lock the high-throughput barrel cover 22;

[0055] Step 6: Repeat Step 4 and Step 5 to complete the operation of multiple high-throughput in-situ fixation barrels 4;

[0056] Step 7: Use the manipulator to hold the lifting handle 5 and put it into the ROV. The ROV starts the hydraulic power, and the oil cylinder 7 acts. Use the blocking plate 8 to squeeze the moving frame 3, and lock the moving frame 3 on the installation base plate 1 through the lower edge plate 9 of the front baffle 6 lock;

[0057] Step eight: Use the ROV to bring back the in-situ high-throughput fixation device for large deep-sea organisms based on the ROV;

[0058] Step nine: After recovery, loosen the oil cylinder 7, the baffle 8 disengages from the moving frame 3, open the high-throughput barrel cover 22, and obtain the sample.

[0059] Through cooperation with the ROV manipulator, the present invention crushes large organisms by extrusion and immerses them in the fixing solution in the high-throughput barrel body 23, and recovers the sample by means of the sealing effect of the high-throughput barrel body 23 during the carrying process. The present invention is less affected by the sampling depth, has strong corrosion resistance, a large storage volume, is convenient to carry, works flexibly and stably, and can quickly and effectively fix large biological samples.

Claims

1. An in-situ high-throughput fixation device for large deep-sea organisms based on ROV, characterized in that: It includes an installation base plate (1), a movable frame (3) and high-throughput in-situ fixing barrels (4). Multiple high-throughput in-situ fixing barrels (4) are placed inside the movable frame (3). The high-throughput barrel bottoms (24) of each high-throughput in-situ fixing barrel (4) are detachably connected to the movable frame (3). The high-throughput barrel lids (22) of each high-throughput in-situ fixing barrel (4) are switchably connected to the high-throughput barrel bodies (23). Each high-throughput in-situ fixing barrel (4) is filled with a fixing liquid. An oil cylinder (7) is installed on the installation base plate (1). After the movable frame (3) is placed on the installation base plate (1), the output end of the oil cylinder (7) abuts against the movable frame (3) to fix the movable frame (3). A base plate frame (2) is fixedly connected to the edge of the installation base plate (1). The oil cylinder (7) is fixed at the rear end of the installation base plate (1). A front baffle (6) is provided on the base plate frame (2) at the front end of the installation base plate (1). A lower edge plate (9) is fixedly connected to the bottom of the movable frame (3). The output end of the oil cylinder (7) is connected to a blocking plate (8) for squeezing the movable frame (3). The height of the blocking plate (8) is higher than that of the lower edge plate (9). When the oil cylinder (7) pushes the movable frame (3), the front baffle (6) and the blocking plate (8) are respectively connected to the lower edge plate (9) by a locking buckle to fix the movable frame (3). The installation base plate (1) is a square flat plate and is placed on the fixed tray of the ROV. The movable frame (3) is a square frame, and the square size is smaller than that of the installation base plate (1). A lifting handle (5) is provided inside the movable frame (3) to facilitate the removal of the movable frame (3) from the installation base plate (1).

2. The in-situ high-throughput fixation device for deep-sea large organisms based on ROV according to claim 1, wherein: Fixing holes (10) are formed at the bottom of the movable frame (3) corresponding to the positions where each high-throughput in-situ fixing barrel (4) is connected. Internal threads are made inside the fixing holes (10). A central hole is formed on the high-throughput barrel bottom (24) of the high-throughput in-situ fixing barrel (4). The high-throughput in-situ fixing barrel (4) is connected to the movable frame (3) through a fixing and sealing bolt (11). One end of the fixing and sealing bolt (11) is hermetically connected to the central hole on the high-throughput barrel bottom (24), and the other end of the fixing and sealing bolt (11) is threadedly connected to the fixing hole (10).

3. The in-situ high-throughput fixation device for deep-sea large organisms based on ROV according to claim 1, wherein: Fixing holes (10) are formed at the bottom of the movable frame (3) corresponding to the positions where each high-throughput in-situ fixing barrel (4) is connected. Internal threads are made inside the fixing holes (10). A groove is formed on the high-throughput barrel bottom (24) of the high-throughput in-situ fixing barrel (4), and internal threads are made inside the groove. The high-throughput in-situ fixing barrel (4) is connected to the movable frame (3) through a double-headed bolt. The two ends of the double-headed bolt are respectively threadedly connected to the fixing hole (10) and the groove.

4. The high-throughput in-situ fixation device for large deep-sea organisms based on ROV according to claim 1, wherein: One end of the high-throughput bucket lid (22) is hinged to the high-throughput bucket body (23), and the other end is provided with a twisting handle (12). A rotating rod (13) is provided on the twisting handle (12). The rotating rod (13) passes through the other end of the high-throughput bucket lid (22) and is rotatably connected to the other end of the high-throughput bucket lid (22). A rotating stop block (14) located below the high-throughput bucket lid (22) is provided at the end of the rotating rod (13). A locking piece (19) is provided on the high-throughput bucket body (23), and a locking hook (21) is fixedly connected to the locking piece (19). When the high-throughput bucket lid (22) is closed, the rotating stop block (14) rotates to the locking hook (21) with the twisting handle (12) to lock the high-throughput bucket lid (22).

5. The in-situ high-throughput fixation device for deep-sea large organisms based on ROV according to claim 4, characterized in that: A clamp (18) is sleeved on the high-throughput bucket body (23), and one end of the high-throughput bucket lid (22) is hinged to the clamp (18). Locking pieces (19) are fixedly connected to both ends of the opening of the clamp (18), and the two locking pieces (19) are locked and fixed by a locking bolt (20). A locking hook (21) is welded to the upper end of any one of the locking pieces (19).

6. The in-situ high-throughput fixation device for deep-sea large organisms based on ROV according to claim 5, characterized in that: A fixed lock block A (15) is fixedly connected to the upper surface of one end of the high-throughput bucket lid (22), and a fixed lock block B (17) is fixedly connected to the clamp (18). The fixed lock block A (15) is connected to the fixed lock block B (17) through a rotating shaft (16).

7. The in-situ high-throughput fixation device for deep-sea large organisms based on ROV according to claim 6, characterized in that: A sealing rubber sheet (25) for sealing the fixing liquid is provided at the top of the high-throughput bucket body (23), and a slit is provided on the sealing rubber sheet (25).

8. A high-throughput in-situ fixation method for deep-sea large organisms based on ROV, characterized in that: Using the ROV-based deep-sea large organism high-throughput in-situ fixing device according to claim 7, the following steps are included Step 1, overall disassembly and cleaning work at the shore-based end. The ROV-based deep-sea large organism high-throughput in-situ fixing device is disassembled and then cleaned. Step 2, assembly after cleaning. In the initial state, the fixing liquid is stored in the high-throughput bucket body (23). Rotate the twisting handle (12), drive the rotating stop block (14) to rotate to the locking hook (21) through the rotating rod (13), lock and close the high-throughput bucket lid (22). Place the moving frame body (3) into the mounting base plate (1). The ROV starts the hydraulic power, the oil cylinder (7) acts, and the moving frame body (3) is squeezed by the blocking plate (8), and the moving frame body (3) is locked on the mounting base plate (1) through the lower edge plate (9) locked by the front baffle (6). Step 3, after being brought to the seabed by the ROV system, relax the oil cylinder (7), the blocking plate (8) disengages from the moving frame body (3), and use the manipulator to hold the lifting handle (5) and take it away from the ROV. Step 4, rotate the twisting handle (12), drive the rotating stop block (14) to disengage from the locking hook (21) through the rotating rod (13), and open the high-throughput bucket lid (22). Use the manipulator to grab a large organism. During the grabbing process, the shell or epidermis of the large organism will be damaged, causing the large organism to be damaged. Step Five: Use a manipulator to place a large organism through the sealing rubber sheet (25) into the high-throughput barrel body (23), and soak the large organism with the fixing liquid inside the high-throughput barrel body (23) to fix the large organism; press the twisting handle (12) to close the high-throughput barrel cover (22), and then rotate the twisting handle (12). Drive the rotating stopper (14) to rotate to the locking hook (21) through the rotating rod (13) to lock the high-throughput barrel cover (22); Step Six: Repeat Step Four and Step Five to complete the operations of multiple high-throughput in-situ fixation barrels (4); Step Seven: Use a manipulator to hold the lifting handle (5) and take in the ROV. The ROV starts the hydraulic power, and the oil cylinder (7) acts. Use the blocking plate (8) to squeeze the moving frame body (3), and lock the moving frame body (3) on the installation base plate (1) through the front baffle (6) locking the lower edge plate (9); Step Eight: Use the ROV to bring back the deep-sea large organism high-throughput in-situ fixation device based on the ROV; Step Nine: After recovery, relax the oil cylinder (7), the blocking plate (8) disengages from the moving frame body (3), open the high-throughput barrel cover (22), and obtain the sample.

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