A device for measuring leech morphology and a measuring method thereof

Abstract

The present invention provides a device for measuring the morphology of leeches and a measuring method thereof. The device comprises a movable support frame, a camera slot, a camera, a fill light, a miniature glass coordinate paper, a miniature glass slot and a computer; the movable support frame comprises a bottom plate, a vertical support and a horizontal support, the vertical support is vertically arranged and fixedly connected to the bottom plate, the horizontal support is movably connected to the vertical support and the horizontal support is arranged parallel to the bottom plate; the camera slot is arranged on the horizontal support, the camera is connected to the computer, the fill light is arranged on the bottom plate, the miniature glass coordinate paper is arranged on the bottom plate through the miniature glass slot; the miniature glass coordinate paper comprises a base glass paper, a bevel glass paper and a top glass paper. The measuring device is used to measure the morphology of leeches, so that the micromorphological characteristics of leeches can be simply, easily and comprehensively observed, the morphological structures of different types of leeches can be distinguished, and a morphological basis can be provided for the classification and systematic development research of leeches.

Description

Technical Field

[0001] The present invention relates to the technical field of biological measurement equipment, and in particular to a device for measuring the morphology of leeches and a measuring method thereof. Background Art

[0002] Leeches are the common name for leeches, belonging to the class Hirudo of the phylum Annelida. They are widely distributed in streams, rivers, lakes, mountains, fields, roadsides, oceans and other aquatic and terrestrial environments on Earth. They mainly feed on the blood or body fluids of humans, livestock, poultry and wild animals. Some species also lead a predatory or saprophytic life. They have very important ecological significance in natural or artificial ecosystems. With the advancement of science and technology, the role of leeches in human social life has become increasingly important. In particular, medical leeches, which are widely used in the field of medicine, have provided a good example for the development and utilization of leeches. Hirudin, secreted by the salivary glands in the mouth of leeches, has many functions such as anticoagulation, and has a very broad application prospect in the clinical treatment of cardiovascular diseases and surgical cosmetic surgery. Therefore, in recent years, the research and protection of leech diversity at home and abroad has become a new research hotspot in the field of life sciences.

[0003] Species identification is the basis for the study and protection of leech diversity, and the collection of morphological data is an essential preliminary work for leech species identification. However, leeches are soft, elastic, and have variable morphologies, making it difficult to measure them in vivo. Specimens soaked in alcohol or formalin have large measurement differences due to different storage times and concentrations of the preservation solution, which is not conducive to comparative studies of shape and size between species.

[0004] Leeches alternately attach themselves to the front and rear suckers, stretching and retracting their bodies, crawling in a worm-like or inchworm-like manner, and their body length usually varies within a few centimeters. Some of their body characteristics, such as body color and stripes, can be directly observed with the naked eye, and some characteristics, such as dorsal sensory organs, papillae, ear-shaped lobes, male and female genital pores, the number and shape of jaws, and the number of body rings, can be directly observed or dissected on fixed specimens (corpses) with the help of a stereoscope. However, how to uniformly and standardizedly describe and record the morphological data of living leeches in a qualitative and quantitative manner and photographic manner has always been a difficult problem that needs to be solved in leech research.

[0005] The industry is in urgent need of a new technology for a device for measuring the morphology of leeches and a method for measuring the same. Summary of the invention

[0006] The purpose of the present invention is to provide a device for measuring the morphology of leeches and a measuring method thereof, so as to facilitate comprehensive and systematic observation of the microscopic morphological characteristics of leeches, so as to realize the unified and standardized qualitative and quantitative description and photographic record of the morphological data of living leeches, and provide a morphological basis for the classification and systematic development research of leeches.

[0007] To achieve the above object, the present invention provides a device for measuring the morphology of leeches, comprising a movable support frame, a camera card slot, a camera, a fill light, a miniature glass coordinate paper, a miniature glass card slot and a computer;

[0008] The movable support frame includes a bottom plate, a vertical support and a horizontal support, wherein the vertical support is vertically arranged and fixedly connected to the bottom plate, and the horizontal support is movably connected to the vertical support and is arranged parallel to the bottom plate;

[0009] The camera slot is arranged on the horizontal bracket and is used to fix the camera;

[0010] The camera is connected to the computer via a data cable, and is used to control the camera to shoot through the computer, which is convenient for observation;

[0011] The fill light is arranged on the bottom plate and is used to enhance the light during the camera shooting process;

[0012] The micro-glass coordinate paper is arranged on the bottom plate through the micro-glass card slot;

[0013] The micro-glass coordinate paper comprises a base glass paper, a bevel glass paper and a top glass paper;

[0014] The base cellophane is a rectangular parallelepiped structure with no cover on the top, used for placing leeches;

[0015] The inclined glass paper is placed on two opposite sides of the base glass paper and has an inclined angle with the bottom surface of the base glass paper;

[0016] The top cellophane is placed on top of the base cellophane in an uncovered position to cover the leeches.

[0017] Furthermore, a blocking portion is provided on a side of the beveled glass paper away from the bottom surface of the base glass paper, for preventing the beveled glass paper from sliding down to the bottom surface of the base glass paper, and the blocking portion is integrally formed with the beveled glass paper.

[0018] Furthermore, a rack is provided on the upper part of the vertical bracket, and a gear meshing with the rack is provided at one end of the horizontal bracket close to the vertical bracket, and the horizontal bracket moves up and down through the mutually meshing gears and racks; a rotating handle connected to the gear axis is provided on the gear, and the horizontal bracket can be moved upward by rotating the rotating handle clockwise, and can be moved downward by rotating the rotating handle counterclockwise.

[0019] Furthermore, the miniature glass card slot includes an iron plate on which the miniature glass coordinate paper can be placed, an iron wire and a column, and the iron plate is movably connected to two columns arranged on the bottom plate through the iron wire.

[0020] Furthermore, the base cellophane, bevel cellophane and top cellophane are all made of transparent glass material that does not stick to water film, and the base cellophane and bevel cellophane have 1mm×1mm square pattern markings, while the top cellophane has no squares; the fill light is a white LED lamp with a color temperature range of 6000-6500K.

[0021] The present invention also provides a method for measuring leech morphology, which uses the above-mentioned device for measuring leech morphology to measure leech morphology, comprising the following steps:

[0022] After placing the leech on the moist base cellophane, cover the top cellophane on the base cellophane; adjust the camera slot position, aim the camera lens at the location of the leech, and then observe the clarity of the interface on the computer display screen, adjust the rotating handle, find the best field of view, and finally adjust the camera lens to focus on the leech and conduct experimental measurements.

[0023] Furthermore, when measuring the body parameters of the leech, when the leech stretches freely and the oral sucker and the tail sucker are attached to the cellophane and do not move, the number of small squares of the base cellophane occupied by the oral sucker and the tail sucker is photographed, and its diameter is calculated by computer software. The width of the middle part of the leech's body at this time is measured as the body width, the width of the leech's head at this time is measured as the head width, and the length of the body at this time is measured as the body length of the leech in its natural state.

[0024] Furthermore, when measuring the back of the leech's body, the clarity of the computer interface was used to manually focus on the leech's head, abdomen, and tail sucker when attached to the cellophane, and the number of eye spots on the head, the distribution pattern, the color of the dorsal stripes, the shape of the dorsal sensilla, the position and shape of the anus, and the shape of the auricular lobe were observed to deduce the area of ​​the leech's back, and calculate the distance and size of the eye spots, the number of dorsal stripes, the number of dorsal sensilla, the density of the dorsal papillae, and the size of the auricular lobe.

[0025] Furthermore, when measuring the body covering of the leech, take out the base cellophane containing the leech, cover the top of the base cellophane with the top cellophane, and then invert the top cellophane and the base cellophane as a whole on the miniature glass card slot, perform secondary focusing through the computer display interface, and then observe the shape of the tail sucker, the shape of the middle jaw of the oral sucker, and the position of the male and female genital openings, calculate the number of radiating ribs on the tail sucker, the number and size of the upper jaws of the oral sucker, the position of the male and female genital openings, and the density of the abdominal papillae, and compare and analyze whether there are differences in the dorsal and abdominal papillae.

[0026] Furthermore, when measuring the side of the leech's body, place the moist base cellophane containing the leech upright in the miniature glass card slot, open the top cellophane, put the inclined cellophane on both sides of the base cellophane, use a brush to gently brush the leech onto the inclined cellophane, cover the top cellophane, and adjust the inclination angle of the iron plate in the miniature glass card slot to find the best position, photograph and observe the side structure of the leech, and calculate the number of renal pores, body rings, and body segments of the leech.

[0027] The present invention has the following beneficial effects:

[0028] 1. A device for measuring the morphology of leeches provided by the present invention comprises a movable support frame, a camera slot, a camera, a fill light, a miniature glass coordinate paper, a miniature glass slot and a computer; the movable support frame comprises a bottom plate, a vertical support and a horizontal support, the vertical support is vertically arranged and fixedly connected to the bottom plate, the horizontal support is movably connected to the vertical support and the horizontal support is arranged parallel to the bottom plate; the camera slot is arranged on the horizontal support for fixing the camera; the camera is connected to the computer via a data cable for controlling the camera to shoot through the computer, so as to facilitate the observation of the morphology of the leeches; the fill light is arranged on the bottom plate The base plate is used to enhance the light during the camera shooting process; the miniature glass coordinate paper is arranged on the base plate through the miniature glass card slot; the miniature glass coordinate paper is arranged on the base plate through the miniature glass card slot; the miniature glass coordinate paper includes base glass paper, inclined glass paper and top glass paper; the base glass paper is a rectangular parallelepiped structure with no cover on the top, which is used to place leeches; the inclined glass paper is placed on the two opposite side surfaces of the base glass paper and has an inclined angle with the bottom surface of the base glass paper; the top glass paper is placed at the uncovered position on the top of the base glass paper, which is used to cover the leeches. The device uses miniature glass coordinate paper and a computer to intuitively display the subtle structure of the leech's body surface on the computer. The structure is clear and the observation is accurate. No stereoscope is required, which can prevent visual fatigue caused by excessive eye use. It is particularly suitable for laboratory operations. At the same time, the computer software can be used to take photos or directly record the features of the back, ventral, side, and front and back suction cups of leeches, so that the subtle differences in the features of various parts of different leech species can be carefully observed, and accurate measurement data of various postures or behavioral states of the leech in a free-moving state can be obtained.

[0029] 2. The device for measuring the morphology of leeches provided by the present invention has a simple structure. The method for measuring the morphology of leeches using the device is simple, direct, and easy to operate. It can more simply, easily, and comprehensively observe the micromorphological characteristics of leeches, and it is easier to distinguish the morphological structures of different types of leeches and accurately classify leeches.

[0030] In addition to the above-described purposes, features and advantages, the present invention has other purposes, features and advantages. The present invention will be further described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The drawings constituting a part of this application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0032] Figure 1 The present invention is a schematic diagram of the overall structure of a device for measuring the morphology of leeches.

[0033] Figure 2 The present invention is a schematic structural diagram of a miniature glass coordinate paper in a device for measuring the morphology of leeches.

[0034] Among them, 1 is a vertical bracket, 2 is a horizontal bracket, 3 is a bottom plate, 4 is a rotating handle, 5 is a miniature glass card slot, 6 is a fill light, 7 is a camera card slot, 8 is a camera, 9 is a computer, 10 is a column, 11 is a wire, A is a top cellophane, B is a base cellophane, and C is a bevel cellophane. DETAILED DESCRIPTION

[0035] The embodiments of the present invention are described in detail below with reference to the accompanying drawings, but the present invention can be implemented in many different ways as defined and covered by the claims.

[0036] See also Figure 1 to Figure 2 , the present invention provides a device for measuring the morphology of leeches, comprising a movable support frame, a camera card slot 7, a camera 8, a fill light 6, a miniature glass coordinate paper, a miniature glass card slot 5 and a computer 9;

[0037] The movable support frame includes a bottom plate 3, a vertical support 1 and a horizontal support 2, wherein the vertical support 1 is vertically arranged and fixedly connected to the bottom plate 3, the horizontal support 2 is movably connected to the vertical support 1 and the horizontal support 2 is parallel to the bottom plate 3;

[0038] The camera slot 7 is provided on the horizontal bracket 2 and is used to fix the camera 8;

[0039] The camera 8 is connected to the computer 9 via a data cable, and is used to control the camera to shoot through the computer, so as to facilitate the observation of the leech morphology;

[0040] The fill light 6 is arranged on the bottom plate 3 and is used to enhance the light during the shooting process of the camera 8;

[0041] The micro-glass coordinate paper is arranged on the bottom plate 3 through the micro-glass card slot 5;

[0042] The micro-glass coordinate paper comprises a base glass paper B, a bevel glass paper C and a top glass paper A;

[0043] The base cellophane B is a rectangular parallelepiped structure without a cover on the top, and is used to place leeches;

[0044] The inclined glass paper C is placed on two opposite sides of the base glass paper B and has an inclined angle with the bottom surface of the base glass paper B;

[0045] The top cellophane A is placed on the uncovered upper portion of the base cellophane B to cover the leeches.

[0046] In a specific embodiment, a blocking portion is provided on a side of the beveled glass paper away from the bottom surface of the base glass paper to prevent the beveled glass paper from sliding to the bottom surface of the base glass paper, and the blocking portion is integrally formed with the beveled glass paper.

[0047] In a specific embodiment, a rack is provided on the upper part of the vertical bracket 1, and a gear meshing with the rack is provided on one end of the horizontal bracket 2 close to the vertical bracket 1, and the horizontal bracket 2 moves up and down through the meshing gear and the rack. A rotating handle 4 connected to the gear axis is provided on the gear, and the horizontal bracket 2 can be moved upward by rotating the rotating handle 4 clockwise, and can be moved downward by rotating the rotating handle 4 counterclockwise. A locking mechanism commonly used in the prior art is also provided between the vertical bracket and the horizontal bracket, and when the position of the horizontal bracket is determined, it is fixed.

[0048] The horizontal bracket 2 is L-shaped and is divided into a horizontal end and a vertical end. The vertical end is connected to the vertical bracket 1 through gear engagement to move up and down. The horizontal end is equipped with a camera slot 7 to fix the camera.

[0049] The miniature glass slot 5 includes an iron plate on which the miniature glass coordinate paper can be placed, an iron wire and a column, and the iron plate is movably connected to two columns 10 arranged on the bottom plate 3 through an iron wire 11. The iron plate of the miniature glass slot 5 can be rotated within 30° to the left and right relative to the vertical direction, so as to observe the side structure of the leech.

[0050] The fill light 6 is a white LED light, which is arranged on two columns 10 and plays a role in enhancing light during the shooting process.

[0051] One side of the camera slot 7 is provided with a hole for the horizontal bracket 2 to pass through, so as to fix the camera slot 7 on the horizontal bracket 2; the other side is provided with a groove matching the size of the camera, and the groove is slightly larger than the camera lens. The camera body is covered with a cloth strip and then stuck in the groove, which can fix the camera.

[0052] The camera lens is a macro lens, the camera is installed on the camera card slot 7, directly connected to the computer display screen, and the camera is controlled to shoot through the computer desktop. The computer includes components such as a host, a display screen, and a keyboard. The computer is used to magnify and observe the various morphological features of leeches, and shoot and record them, thereby relieving work fatigue caused by long-term observation.

[0053] The micro-glass coordinate paper used in the leech morphology measurement method of the present invention is as follows: Figure 2 As shown:

[0054] The top cellophane A is 15.2cm×15.2cm in size, 1mm thick, and has no grids. It is used to cover the back and sides of the leech to prevent the leech from escaping the cellophane due to excessive movement.

[0055] The base of the glass paper B is 15cm×15cm in size and 2mm thick, with a 1mm×1mm square grid, and a 2cm groove is left around to observe the back and side structures of the leech. At the same time, it keeps the leech moist and prevents the leech from moving too much.

[0056] The inclined glass paper C is 15cm×4cm in size and 1mm thick. It has a 1mm×1mm grid, with 1cm left on one side to make it vertical. During the experimental observation process, it is placed on the edge of the base glass paper groove and the angle of the micro-glass slot is appropriately adjusted to observe the side structure of the leech.

[0057] The present invention also provides a method for measuring leeches using the above device, comprising the following steps:

[0058] Use screws to fix the bottom plate and the vertical bracket, place it on the test bench, take out the horizontal bracket and press Figure 1 As shown, it is installed on the vertical bracket, and the vertical bracket is moved downward by rotating the rotating handle counterclockwise, and the camera card slot is connected to the horizontal end of the horizontal bracket at the same time;

[0059] Install the camera into the groove of the camera card slot, connect the camera to the computer desktop via the USB interface, and open the computer desktop;

[0060] Plug in the fill light and adjust the brightness of the bulb according to the light intensity in the laboratory;

[0061] Use a brush dipped in water to rinse the inside of the base cellophane to keep it moist, then place the base cellophane on the micro-glass card slot, keeping the micro-glass card slot and the micro-glass card slot in a horizontal state (when measuring the body parameters of the leech, the back of the leech body and the leech body cover, the length of the iron wire can be shortened to keep the iron plate in the micro-glass card slot fixed; when measuring the side of the leech body, increase the length of the iron wire to keep the iron plate in the micro-glass card slot rotated within 30° relative to the vertical direction, so as to observe the side structure of the leech);

[0062] Place the leech inside the moist base cellophane and cover the base cellophane with the top cellophane to prevent the leech from escaping from the base cellophane;

[0063] Manually adjust the position of the camera slot on the horizontal bracket, aim the camera lens at the location of the leech, then observe the clarity of the interface on the computer screen, turn the rotary handle to find the best field of view, and finally adjust the camera lens to focus on the leech and perform experimental measurements.

[0064] Measurement of leech body parameters: When the leech stretches freely and its oral and tail suckers are stuck to the cellophane, take a picture of the number of small squares of the cellophane occupied by the oral and tail suckers at the base, calculate its diameter using computer software, and measure the width of the middle part of the leech's body as the body width, the width of the leech's head as the head width, and the length of the body as the body length of the leech in its natural state.

[0065] Measurement of the back of the leech: Through the clarity of the computer interface, manually focus on the leech's head, abdomen, and tail sucker when attached to the cellophane, observe the number of eye spots on the head, distribution pattern, dorsal stripe color, shape of dorsal sensory organ, position and shape of anus, shape of auricular lobe, calculate the area of ​​the leech's back, calculate the distance and size of the eye spots, number of dorsal stripes, number of dorsal sensory organs, density of dorsal papillae, and size of auricular lobe.

[0066] To measure the body cover of the leech: Take out the base cellophane containing the leech, cover the top of the base cellophane with the top cellophane, and then invert the top cellophane and base cellophane as a whole. Figure 1 On the miniature glass slot shown in the figure, manually turn the rotating handle, perform secondary focusing through the computer display interface, and then observe the shape of the caudal sucker, the shape of the middle jaw of the oral sucker, and the position of the male and female genital openings, calculate the number of radiating ribs on the caudal sucker, the number and size of the upper jaws of the oral sucker, the position of the male and female genital openings, and the density of abdominal papillae, and compare and analyze whether there are differences between the dorsal and abdominal papillae.

[0067] Measuring the side of the leech's body: Place the base cellophane upright in the miniature glass slot, put the beveled cellophane into the edge of the base cellophane groove, use a brush to gently sweep the leech onto the beveled cellophane, and then cover it with the top cellophane. Adjust the inclination of the miniature glass slot appropriately to find the best position, photograph and observe the side structure of the leech, and calculate the number of renal pores, body rings, and body segments of the leech.

[0068] The invention provides a device for measuring the morphology of leeches. The device uses miniature glass coordinate paper and a computer to intuitively display the fine structure of the leech body surface on the computer. The device has a clear structure and accurate observation, does not need a stereoscope, can prevent visual fatigue caused by excessive use of eyes, and is particularly suitable for laboratory operations. At the same time, the features of the back, ventral, side, and front and rear suckers of leeches can be photographed or directly recorded through computer software, so that the fine differences in the features of various parts of different leech species can be carefully observed, and accurate measurement data of various postures or behavior states of the leech body in a free activity state can be obtained.

[0069] The device for measuring the morphology of leeches provided by the present invention has a simple structure. The method for measuring the morphology of leeches using the device is simple, direct, and easy to operate. The micromorphological characteristics of leeches can be observed more simply, easily, and comprehensively, and the morphological structures of different types of leeches can be distinguished more easily, so that leeches can be classified accurately.

[0070] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A device for measuring leech morphology, characterized in that: It includes a movable support frame, a camera card slot, a camera, a fill light, a micro-glass coordinate paper, a micro-glass card slot and a computer; The movable support frame includes a bottom plate, a vertical support and a horizontal support, wherein the vertical support is vertically arranged and fixedly connected to the bottom plate, and the horizontal support is movably connected to the vertical support and is arranged parallel to the bottom plate; The camera slot is arranged on the horizontal bracket and is used to fix the camera; The camera is connected to the computer via a data cable, and is used to control the camera to shoot through the computer, so as to facilitate observation of the leech morphology; The fill light is arranged on the bottom plate and is used to enhance the light during the camera shooting process; The micro-glass coordinate paper is arranged on the bottom plate through the micro-glass card slot; The micro-glass coordinate paper comprises a base glass paper, a bevel glass paper and a top glass paper; The base cellophane is a rectangular parallelepiped structure with no cover on the top, used for placing leeches; The inclined glass paper is placed on two opposite sides of the base glass paper and has an inclined angle with the bottom surface of the base glass paper; The top cellophane is placed on the uncovered upper part of the base cellophane to cover the leech; A blocking portion is provided on a side of the bevel glass paper away from the bottom surface of the base glass paper, for preventing the bevel glass paper from sliding down to the bottom surface of the base glass paper, and the blocking portion is integrally formed with the bevel glass paper; A rack is provided on the upper part of the vertical bracket, and a gear meshing with the rack is provided at one end of the horizontal bracket close to the vertical bracket. The horizontal bracket moves up and down through the meshing gears and racks; a rotating handle connected to the gear axis is provided on the gear, and the horizontal bracket can be moved upward by rotating the rotating handle clockwise, and can be moved downward by rotating the rotating handle counterclockwise.

2. A device for measuring leech morphology according to claim 1, characterized in that: The miniature glass card slot comprises an iron plate on which the miniature glass coordinate paper can be placed, an iron wire and a column, and the iron plate is movably connected to two columns arranged on the bottom plate through the iron wire.

3. A device for measuring leech morphology according to claim 1, characterized in that: The base cellophane, bevel cellophane and top cellophane are all made of transparent glass material that does not stick to water film, and the base cellophane and bevel cellophane have 1mm×1mm square pattern markings, while the top cellophane has no squares; the fill light is a white LED lamp with a color temperature range of 6000-6500K.

4. A method for measuring leech morphology, characterized in that: The leech morphology measurement is performed using a device for measuring leech morphology as claimed in any one of claims 1 to 3, comprising the following steps: After placing the leech on the moist base cellophane, cover the top cellophane on the base cellophane; adjust the camera slot position, aim the camera lens at the location of the leech, and then observe the clarity of the interface on the computer display screen, adjust the rotating handle, find the best field of view, and finally adjust the camera lens to focus on the leech and conduct experimental measurements.

5. A method for measuring leech morphology according to claim 4, characterized in that: When measuring the body parameters of the leech, when the leech stretches freely and its oral sucker and tail sucker are attached to the cellophane and do not move, take a picture of the number of small squares of the base cellophane occupied by the oral sucker and tail sucker at this time, calculate its diameter through computer software, and measure the width of the middle part of the leech's body as the body width, the width of the leech's head as the head width, and the length of the body at this time as the body length of the leech in its natural state.

6. A method for measuring leech morphology according to claim 4, characterized in that: When measuring the back of the leech's body, use the clarity of the computer interface to manually focus on the leech's head, abdomen, and tail sucker attached to the cellophane, observe the number of eye spots on the head, distribution pattern, dorsal stripe color, shape of dorsal sensory organ, position and shape of anus, shape of auricular lobe, deduce the area of ​​the leech's back, calculate the distance and size of the eye spots, number of dorsal stripes, number of dorsal sensory organs, density of dorsal papillae, and size of auricular lobe.

7. A method for measuring leech morphology according to claim 4, characterized in that: When measuring the abdomen of the leech, take out the base cellophane containing the leech, cover the top of the base cellophane with the top cellophane, and then invert the top cellophane and the base cellophane as a whole on the miniature glass card slot, and then perform a second focusing through the computer display interface, and then observe the shape of the tail sucker, the shape of the middle jaw of the oral sucker, and the position of the male and female genital openings, calculate the number of radial ribs on the tail sucker, the number and size of the upper jaws of the oral sucker, the position of the male and female genital openings, and the density of abdominal papillae, and compare and analyze whether there are differences between the dorsal and abdominal papillae.

8. A method for measuring leech morphology according to claim 4, characterized in that: When measuring the side of the leech's body, place the moist base cellophane containing the leech upright in the miniature glass slot, open the top cellophane, put the inclined cellophane on both sides of the base cellophane, use a brush to gently sweep the leech onto the inclined cellophane, cover the top cellophane, and adjust the inclination angle of the iron plate in the miniature glass slot to find the best position, photograph and observe the side structure of the leech, and calculate the number of renal pores, body rings, and body segments of the leech.

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