A centrifugal microfluidic chip array valve structure

Abstract

The present invention discloses a centrifugal microfluidic chip dot matrix valve structure, comprising a first fluid channel, a second fluid channel, and at least one dot matrix sequence group disposed on a chip substrate. The first fluid channel is wider than the second fluid channel, and the first and second fluid channels are connected and communicated via at least one dot matrix sequence group therebetween. The dot matrix sequence group comprises a plurality of evenly arranged dot matrix structures, with dot matrix gaps formed between adjacent dot matrix structures. The present invention combines the functions of fluid quantification under low-speed centrifugation with the functions of releasing fluid by breaking through the obstruction of the dot matrix sequence group under high-speed centrifugation. The array structures are evenly arranged, providing a filtering function. The microchannel design has a microcapillary function, which can enhance the mixing effect of the fluid. The dot matrix gaps and flow channel design can prevent liquid backflow.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and more particularly to a centrifugal microfluidic chip matrix valve structure. Background Art

[0002] A centrifugal microfluidic chip refers to a microfluidic system that uses micro-electromechanical processing technology to miniaturize valves, flow pipes, mixing reactors, heaters, separation devices, detectors and other components involved in the sampling, pretreatment, derivatization, mixing and detection processes of chemical analysis, and integrates them into a CD-shaped chip. It uses centrifugal force as the driving force for liquid flow to realize liquid flow detection and analysis.

[0003] However, the valve functions of existing centrifugal microfluidic chips are single and cannot meet usage requirements.

[0004] Therefore, providing a multifunctional centrifugal microfluidic chip array valve structure is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a centrifugal microfluidic chip array valve structure, which combines multiple functions such as quantification under low-speed centrifugation, release under high-speed centrifugation, filtration and mixing.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A centrifugal microfluidic chip dot matrix valve structure includes a first fluid channel, a second fluid channel and at least one dot matrix sequence group arranged on a chip substrate, wherein the width of the first fluid channel is greater than the width of the second fluid channel, and the first fluid channel and the second fluid channel are connected to each other through at least one of the dot matrix sequence groups therebetween.

[0008] By adopting the above scheme, the beneficial effects of the present invention are:

[0009] 1) It has the function of both fluid quantification under low-speed centrifugation and breaking through the barrier of the lattice sequence group to release fluid under high-speed centrifugation;

[0010] 2) The microchannel design has micro-capillary function, which can improve the mixing effect of the fluid.

[0011] Furthermore, a resistance groove is provided on the chip substrate, and the resistance groove is located at the bottom of any of the dot matrix sequence groups.

[0012] Furthermore, the lattice sequence group includes a plurality of evenly arranged lattice structures, and lattice gaps are formed between adjacent lattice structures; the first fluid channel and the second fluid channel are connected to each other through the plurality of lattice gaps.

[0013] The beneficial effect of adopting the above-mentioned further technical solution is that multiple lattice structures are evenly arranged and have a filtering function; at the same time, the lattice gap and flow channel design can prevent liquid backflow.

[0014] Furthermore, the dot matrix sequence group is hourglass-shaped, triangular, polygonal, arc-shaped, or fence-shaped; and the dot matrix structure is circular, square, or triangular.

[0015] Furthermore, the resistance groove is circular or square.

[0016] Furthermore, the width of the first fluid channel is 1-2.5 mm; the width of the second fluid channel is 0.1-1 mm; the width of the lattice gap is 0.1-0.3 mm; the radius of the lattice structure is 0.1-0.3 mm or the side length of the lattice structure is 0.1-1 mm; the depth of the first fluid channel and the second fluid channel is 0.1-1 mm.

[0017] Furthermore, the resistance groove is circular with a radius of 0.2-0.5 mm, and a depth of the resistance groove is 0.5-2 mm.

[0018] It can be seen from this that the present invention provides a centrifugal microfluidic chip array valve structure. Compared with the existing technology, the present invention combines multiple functions such as quantification under low-speed centrifugation, release under high-speed centrifugation, filtration and mixing, and can be used in multiple fields such as chemical analysis, material preparation, and biological sample separation and recovery. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0020] Figure 1 The accompanying drawing is a schematic structural diagram of a centrifugal microfluidic chip array valve structure in Example 1 of the present invention;

[0021] Figure 2 The accompanying drawing is a schematic structural diagram of a centrifugal microfluidic chip array valve structure in Example 1 of the present invention when in use;

[0022] Figure 3 The accompanying drawing is a schematic structural diagram of a centrifugal microfluidic chip array valve structure in Example 2 of the present invention;

[0023] Figure 4 The accompanying drawing is a structural schematic diagram of a centrifugal microfluidic chip array valve structure in Example 2 of the present invention when in use. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Example 1:

[0026] like Figure 1-2 As shown, the embodiment of the present invention discloses a centrifugal microfluidic chip dot array valve structure, comprising a first fluid channel 1, a second fluid channel 2, and a dot array sequence group 3 provided on the chip substrate, wherein the width of the first fluid channel 1 is greater than the width of the second fluid channel 2, and the first fluid channel 1 and the second fluid channel 2 are connected and communicated through the dot array sequence group 3 therebetween. In this embodiment, the upper portion of the first fluid channel 1 is connected to a quantitative reservoir 4, and the lower portion of the second fluid channel 2 is connected to another liquid storage reservoir 5 or the outside of the chip. The distance between the dot array sequence group 3 and the center of the centrifugal shaft 6 is 35-60 mm. Under centrifugal conditions of a centrifugal speed of 300-600 r / min and a centrifugal time of 5-180 s, fluid quantification can be achieved. Under centrifugal conditions of a centrifugal speed of ≥1500 r / min and a centrifugal time of ≥5 s, the fluid can break through the resistance of the dot array sequence group 3 and transfer into the liquid storage reservoir 5 below. Thus, the present invention has the functions of fluid quantification under low-speed centrifugation and breaking through the obstruction of the dot array sequence group 3 to release fluid under high-speed centrifugation. At the same time, the microchannel design has a microcapillary function, which can improve the mixing effect of the fluid.

[0027] Specifically, the dot matrix sequence group 3 includes a plurality of evenly arranged dot matrix structures 31 , which are protruding structures protruding from the horizontal plane of the chip substrate, and dot matrix gaps 32 are formed between adjacent dot matrix structures 31 ; the first fluid channel 1 and the second fluid channel 2 are connected to each other through the plurality of dot matrix gaps 32 .

[0028] Specifically, the dot array sequence group 3 is hourglass-shaped, triangular, polygonal, arc-shaped, or fence-shaped; the dot array structure 31 is circular, square, or triangular. In this embodiment, the dot array sequence group 3 is hourglass-shaped, and the dot array structure 31 is circular.

[0029] Specifically, the width of the first fluid channel 1 is 1-2.5 mm; the width of the second fluid channel 2 is 0.1-1 mm; the width of the lattice gap 32 is 0.1-0.3 mm; the radius of the lattice structure 31 is 0.1-0.3 mm; and the depth of the first fluid channel 1 and the second fluid channel 2 is 0.1-1 mm. In this embodiment, the width of the first fluid channel 1 is 1-2 mm, and the width of the second fluid channel 2 is 0.1-0.7 mm.

[0030] The dot matrix valve structure of the present invention can be prepared on a glass, ceramic or polymer (polystyrene, cycloolefin copolymer, polycarbonate, polymethacrylate, PE, PDMS, TPE, etc.) chip substrate by laser cutting, scanning, mold hot pressing, mold injection molding, CNC processing or other replication methods, and can be sealed with medical single-sided tape, pressure-sensitive film, seamless tape or transparent tape.

[0031] Example 2:

[0032] like Figure 3-4 As shown, an embodiment of the present invention discloses a centrifugal microfluidic chip dot matrix valve structure, including a first fluid channel 01 and a second fluid channel 02 provided on a chip substrate, and two dot matrix sequence groups provided on the chip substrate, namely a first dot matrix sequence group 03 and a second dot matrix sequence group 04. The width of the first fluid channel 01 is greater than that of the second fluid channel 02, and the first fluid channel 01 and the second fluid channel 02 are connected to each other through the two dot matrix sequence groups therebetween. In this embodiment, the upper portion of the first fluid channel 01 is connected to the quantitative pool 05, and the lower portion of the second fluid channel 02 is connected to another liquid storage pool 06 or the outside of the chip. The distance between the first dot matrix sequence group 03 and the center of the centrifugal axis 07 is 20-60 mm. Fluid quantification can be achieved under centrifugal conditions of a centrifugal rate of 300-800 r / min and a centrifugal time of 5-180 s. Under centrifugal conditions of a centrifugal rate ≥2000 r / min and a centrifugal time ≥5 s, the fluid can break through the resistance of the two dot matrix sequence groups and transfer into the liquid storage pool 06 below. Therefore, the present invention has both the function of fluid quantification under low-speed centrifugation and the function of breaking through the resistance of the two dot matrix sequence groups to release fluid under high-speed centrifugation. The microchannel design has a microcapillary function, which can improve the mixing effect of the fluid.

[0033] Specifically, a resistance groove 08 is further provided on the chip substrate. The resistance groove 08 is located at the bottom of the second dot array sequence group 04 to form a resistance valve.

[0034] Specifically, the first dot lattice sequence group 03 and the second dot lattice sequence group 04 have the same structure, both including multiple evenly arranged dot lattice structures 031, with dot lattice gaps 032 formed between adjacent dot lattice structures 031; the first fluid channel 01 and the second fluid channel 02 are connected to each other through the multiple dot lattice gaps 032, and the resistance groove 08 is located at the bottom of the multiple dot lattice structures 031 of the second dot lattice sequence group 04.

[0035] Specifically, the first dot sequence group 03 and the second dot sequence group 04 are both hourglass-shaped, triangular, polygonal, arc-shaped, or fence-shaped; the dot structure 031 is circular, square, or triangular. In this embodiment, the first dot sequence group 03 and the second dot sequence group 04 are both fence-shaped, and the dot structure 031 is square.

[0036] Specifically, the resistance groove is circular or square. In this embodiment, the resistance groove is circular.

[0037] Specifically, the width of the first fluid channel 01 is 1-2.5 mm; the width of the second fluid channel 02 is 0.1-1 mm; the width of the lattice gap 032 is 0.1-0.3 mm; the side length of the lattice structure 031 is 0.1-1 mm; and the depth of the first fluid channel 01 and the second fluid channel 02 is 0.1-1 mm. In this embodiment, the width of the first fluid channel 01 is 1-2.5 mm.

[0038] Specifically, the radius of the resistance groove 08 is 0.2-0.5 mm, and the depth of the resistance groove 08 is 0.5-2 mm.

[0039] The dot matrix valve structure of the present invention can be prepared on a glass, ceramic or polymer (polystyrene, cycloolefin copolymer, polycarbonate, polymethacrylate, PE, PDMS, TPE, etc.) chip substrate by laser cutting, scanning, mold hot pressing, mold injection molding, CNC processing or other replication methods, and can be sealed with medical single-sided tape, pressure-sensitive film, seamless tape or transparent tape.

[0040] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. Reference can be made to the common and similar parts between the various embodiments. For the devices disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the method description.

[0041] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A centrifugal microfluidic chip array valve structure, characterized in that: The chip substrate includes a first fluid channel, a second fluid channel, and at least one dot array sequence group. The width of the first fluid channel is greater than the width of the second fluid channel. The first fluid channel and the second fluid channel are connected to each other through at least one of the dot array sequence groups. The chip substrate also includes a resistance groove, which is located at the bottom of any of the dot array sequence groups. The distance between the dot array sequence group and the center of the centrifugal axis is 35-60 mm. Fluid quantification can be achieved under centrifugal conditions of 300-600 r / min and 5-180 s. Under centrifugal conditions of ≥1500 r / min and ≥5 s, the fluid can break through the resistance of the dot array sequence group and transfer into the liquid storage tank below. Or the distance between the first dot array sequence group and the center of the centrifugal axis is 20-60 mm, and fluid quantification can be achieved under centrifugal conditions of a centrifugal rate of 300-800 r / min and a centrifugal time of 5-180 s. Under centrifugal conditions of a centrifugal rate ≥2000 r / min and a centrifugal time ≥5 s, the fluid can break through the resistance of the two dot array sequence groups and transfer into the liquid storage pool below.

2. A centrifugal microfluidic chip array valve structure according to claim 1, characterized in that: The lattice sequence group includes a plurality of evenly arranged lattice structures, with lattice gaps formed between adjacent lattice structures; the first fluid channel and the second fluid channel are connected to each other through the plurality of lattice gaps.

3. A centrifugal microfluidic chip array valve structure according to claim 2, characterized in that: The dot matrix sequence group is in the shape of an hourglass, a triangle, a polygon, an arc, or a fence; and the dot matrix structure is in the shape of a circle, a square, or a triangle.

4. The centrifugal microfluidic chip array valve structure according to claim 1, characterized in that: The resistance groove is circular or square.

5. The centrifugal microfluidic chip array valve structure according to claim 3, characterized in that: The width of the first fluid channel is 1-2.5 mm; the width of the second fluid channel is 0.1-1 mm; the width of the lattice gap is 0.1-0.3 mm; the radius of the lattice structure is 0.1-0.3 mm or the side length of the lattice structure is 0.1-1 mm; the depth of the first fluid channel and the second fluid channel is 0.1-1 mm.

6. The centrifugal microfluidic chip array valve structure according to claim 4, characterized in that: The resistance groove is circular with a radius of 0.2-0.5 mm, and a depth of 0.5-2 mm.

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