A biological cell reactor

Abstract

This invention relates to a container for biochemical reactions, and more particularly to a biological cell reactor, comprising a reactor, a rotating shaft (1), two sliding shafts (9), and a stirring plate (5); the stirring plate has a rotating hole (11) with internal threads in the middle, and sliding holes (10) on both sides that match the sliding shafts (9), the internal threads matching the external threads, and the diameter of the sliding holes (10) being slightly larger than the diameter of the sliding shafts. The beneficial effects of this invention are that, through the up-and-down movement of the stirring plate, a better stirring effect is achieved at a slow speed, allowing the materials to mix more quickly and avoiding damage to the cells; since the rotating shaft rotates at a relatively slow speed, only the sliding sleeve is needed to achieve the rotation of the rotating shaft.

Description

Technical Field

[0001] This invention relates to a container for biochemical reactions, and more particularly to a biological cell reaction vessel. Background Technology

[0002] Biochemical reactions, especially cell culture, require aseptic conditions and a high degree of environmental cleanliness. Furthermore, cell reactions necessitate thorough mixing of the reaction solution; the culture medium must have a certain degree of fluidity, but not be too vigorous to prevent cell damage.

[0003] Currently, reaction vessels used for cell growth generally employ anchor-type stirrers to ensure thorough mixing of cells and nutrients. However, this type of reactor has several drawbacks: firstly, the rapid stirring speed can easily damage cells; secondly, the stirring effect is poor, as the liquid only moves circumferentially and cannot exchange the culture medium vertically; and thirdly, the area above the stirrer shaft requires lubricating oil and sealing packing, which can easily contaminate the cells and hinder cell growth. Summary of the Invention

[0004] To overcome the above-mentioned shortcomings, the present invention provides a biological cell reactor to overcome the deficiencies of the current technology, improve the stirring effect, prevent cell contamination, and promote cell growth.

[0005] A biological cell reactor, comprising a reactor, characterized in that: it further comprises,

[0006] The rotating shaft is located in the middle of the reactor. Its top end extends out of the reactor and is connected to a motor. The upper end is fixed to the reactor by an upper sliding sleeve, and the lower end is fixed in a groove at the bottom of the reactor by a lower sliding sleeve. The diameters of the upper and lower sliding sleeves are equal to the diameter of the rotating shaft, and external threads are provided on the outer circumference.

[0007] Two sliding shafts are fixed inside the reactor and located on both sides of the rotating shaft; a forward switch is provided at the bottom to make the motor run in the forward direction, and a reverse switch is provided at the top to make the motor rotate in the reverse direction.

[0008] The mixing disc has a rotating hole with internal threads in the middle and sliding holes on both sides that match the sliding shaft. The internal threads match the external threads, and the diameter of the sliding holes is slightly larger than the diameter of the sliding shaft.

[0009] In the aforementioned biological cell reactor, preferably, to prevent turbulence and ensure the smooth up-and-down movement of the stirring plate, four flow holes with the same diameter are provided in the center of the stirring plate and are evenly distributed around the circumference.

[0010] In the aforementioned biological cell reactor, preferably, the diameter of the stirring plate is 0.3-0.5 times the true diameter of the reactor.

[0011] In the aforementioned biological cell reactor, preferably, the sliding shaft, rotating shaft, and stirring plate are made of stainless steel or engineering plastics.

[0012] In the aforementioned biological cell reactor, preferably, the motor is a continuously variable speed motor.

[0013] In this invention, the rotating shaft rotates via an upper and lower sliding sleeve. As the rotating shaft rotates, the stirring disc moves up and down via a threaded connection. When the stirring disc is at the lower end of the rotating shaft and contacts the forward switch of the sliding sleeve, the rotating shaft rotates in the forward direction, driving the stirring disc to move along the rotating shaft. When the upper reverse switch is activated, the motor rotates in the reverse direction, and the stirring disc moves downward. When the lower forward switch is activated, the disc moves upward, and the cycle repeats continuously.

[0014] Because the stirring disc is a horizontal disc, when the stirring disc moves upward, it can drive the reaction liquid above the stirring disc upward, drive the reaction liquid around the stirring disc downward, and drive the reaction liquid at the bottom of the reactor upward, completing the exchange of the reaction liquid. When the stirring disc moves downward, the reaction liquid moves in the opposite direction. Each cycle of the stirring disc allows the reaction liquid to move up and down once, ensuring good mixing of the reaction liquid. The stirring disc of this invention achieves excellent results even when operating at slow speed.

[0015] Four flow holes are located in the middle of the stirring plate, allowing some of the reaction liquid to pass through, ensuring smooth up-and-down movement of the stirring plate. This prevents turbulence in the reaction liquid and keeps the stirring plate as horizontal as possible.

[0016] The beneficial effects of this invention are that, through the up-and-down movement of the stirring disc, a better stirring effect is achieved at a slow speed, allowing the materials to be mixed more quickly and avoiding damage to the cells; since the rotation speed of the rotating shaft is relatively slow, only a sliding sleeve is needed to achieve the rotation of the rotating shaft. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the reaction vessel in Embodiment 1 of the present invention.

[0018] Figure 2 This is a top view of the stirring plate in Embodiment 2 of the present invention.

[0019] In the diagram, 1 is the rotating shaft, 2 is the motor, 3 is the upper sliding sleeve, 4 is the lower sliding sleeve, 5 is the stirring plate, 6 is the stop switch, 7 is the run switch, 8 is the flow hole, 9 is the sliding shaft, 10 is the sliding hole, and 11 is the rotating hole. Detailed Implementation

[0020] A biological cell reactor of the present invention, such as Figure 1 and Figure 2 As shown.

[0021] Inside the reactor, there is a rotating shaft 1 with external threads. A motor 2 is fixed at the top. The upper end of the rotating shaft is fixed to the top of the reactor via an upper sliding sleeve 3. The lower end of the rotating shaft is placed in a groove at the bottom of the reactor. A lower sliding sleeve 4 is installed in the groove. The diameters of the upper and lower sliding sleeves are equal to the diameter of the rotating shaft, so that the rotating shaft can rotate in both directions under the drive of the motor.

[0022] There is a sliding shaft 9 on each side of the rotating shaft. The sliding shafts are parallel to the rotating shaft and are fixed inside the reactor by a bracket. A forward switch 7 is provided at the lower part of the sliding shaft to make the motor run in the forward direction, and a reverse switch 6 is provided at the upper part to make the motor rotate in the reverse direction.

[0023] A stirring disc 5 is mounted on both the rotating shaft and the sliding shaft. The diameter of the stirring disc is 0.4 times the true diameter of the reactor. A rotating hole 11 is located in the center of the stirring disc 5, and the rotating hole 11 has an internal thread that matches the external thread on the rotating shaft. When the rotating shaft rotates, it drives the stirring disc to move up and down. Two sliding holes 10 are located on either side of the rotating hole 11 of the stirring disc 5. The diameter of the sliding holes is slightly larger than the diameter of the sliding shaft, allowing the stirring disc to move up and down on the sliding shaft. The stirring disc also has four flow holes 8, all of the same diameter and evenly distributed circumferentially. When the stirring disc moves up and down, a small amount of solution can flow through the flow holes, preventing turbulence in the reactor and allowing the stirring disc to move smoothly upwards or downwards.

[0024] A forward switch 6 is provided on the upper part of the sliding shaft to make the motor run in the forward direction, and a reverse switch 7 is provided on the lower part of the sliding shaft to make the motor run in the reverse direction.

[0025] The reactor, rotating shaft, stirring plate, and sliding shaft are all made of stainless steel. The motor is a continuously variable speed motor.

[0026] The reactor is also equipped with a feed inlet, a discharge outlet, and an inspection port.

[0027] When the motor is powered on, and the stirring disc is at the lower end of the rotating shaft, contacting the forward switch of the sliding shaft, the rotating shaft rotates in the forward direction, driving the stirring disc to move along the rotating shaft. When it reaches the upper reverse switch, the motor rotates in the reverse direction, and the stirring disc moves downward. When it reaches the lower forward switch, it moves upward, and the cycle repeats continuously.

[0028] When the stirring disc moves upward or downward, it moves the solution inside the reactor up and down, ensuring thorough mixing. The speed of the stirring disc can be controlled by adjusting the motor power.

Claims

1. A biological cell reactor, comprising a reactor, characterized in that: It also includes, The rotating shaft (1) is located in the middle of the reactor. The top of the shaft extends out of the reactor and is connected to a motor (2). The upper end is fixed to the reactor by the upper sliding sleeve (3), and the lower end is fixed in the groove at the bottom of the reactor by the lower sliding sleeve (4). The diameters of the upper and lower sliding sleeves are equal to the diameter of the rotating shaft, and the outer circumference is provided with external threads. Two sliding shafts (9) are fixed inside the reactor and located on both sides of the rotating shaft respectively; a forward switch (7) is provided at the bottom to make the motor run in the forward direction, and a reverse switch (6) is provided at the top to make the motor rotate in the reverse direction. The mixing plate (5) has a rotating hole (11) with internal threads in the middle and sliding holes (10) on both sides that match the sliding shaft (9). The internal threads match the external threads, and the diameter of the sliding hole (10) is slightly larger than the diameter of the sliding shaft. The diameter of the stirring plate is 0.3-0.5 times the diameter of the reactor; The mixing plate has four flow holes (8) in the middle, and the flow holes have the same diameter and are evenly distributed around the circumference.

2. The biological cell reactor according to claim 1, characterized in that: The sliding shaft, rotating shaft, and stirring plate are made of stainless steel or engineering plastics.

3. A biological cell reactor according to claim 1, characterized in that: The motor is a continuously variable transmission (CVT) motor.

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